KR102120323B1 - Composition for hepatoprotective or ameliorating hangover containing herbal extract and edible insect enzyme-decomposed with enzyme - Google Patents

Abstract

The present invention relates to a composition containing extracts of composition enzyme-decomposed mealworms, enzyme-decomposed Protaetia brevitarsis seulensis (Kolbe) larvae, enzyme-decomposed crickets, a matured pumpkin extract, a cinnamon extract, a Citrus unshiu Markovich peel extract, an Astragalus membranaceus Bunge extract, a fermented Green flesh black bean extract, a Lepidium meyenii extract, a ginseng flower bud extract, a grape extract, and a Scutellaria baicalensis Georgi extract. According to the present invention, the composition provides excellent hangover relief due to activity increase of alcohol decomposition enzyme and mitigates hepatoxicity caused by alcohol, thereby being easily used as a treatment composition for various kinds of diseases, such as alcoholic hepatitis and alcohol fatty liver related to liver damage and health function food for preventing and mitigating the diseases.

Description

Composition for hepatoprotective or ameliorating hangover containing herbal extract and edible insect enzyme-decomposed with enzyme}

The present invention relates to a composition for relieving hangover or liver protection containing an edible insect enzyme treatment and a herbal extract, more specifically, a brown larva larva enzyme treatment, a white spotted flower larva larva enzyme treatment, a cricket adult enzyme treatment, an old Pumpkin extract, cinnamon extract, dermis extract, astragalus extract, germinated frost extract, maca extract, ginseng torpedo extract, grape extract, relates to a composition for relieving hangover or protecting liver containing golden extract.

Liver disease mainly refers to various liver diseases caused by chronic drinking. It is largely divided into alcoholic fatty liver, alcoholic hepatitis, and alcoholic cirrhosis, depending on the type, but it is rare that only one disease occurs in one person, and each disease varies from person to person. Will appear.

According to statistics from the Health Insurance Review and Assessment Service, treatment costs incurred by domestic alcoholic liver disease have been increasing rapidly every year. In addition, when analyzing the statistics of a domestic life insurance company, the number of deaths from alcoholic liver disease among the causes of skyrocketing has increased seven times compared to 10 years ago.

Alcoholic liver disease caused by continuous alcohol consumption has emerged as a social problem worldwide, including in Korea. In particular, if alcoholic fatty liver and hepatitis persist, liver fibrosis and cirrhosis can no longer be expected to recover, and liver transplantation remains the only treatment.

In Korea, the drinking rate of adult males over the age of 20 is 83.3%, and the drinking rate of women is also rapidly increasing to 54.9%, and it is severely exposed to excessive drinking due to the wrong drinking culture. The damage caused by excessive consumption of alcohol not only causes etiology of various diseases including liver disease, but also negatively affects the emotions and lives of individuals, families, and society, causing great economic and social loss in the country.

Among the various types of side effects caused by excessive drinking, hangover is a symptom of thirst, general boredom, fatigue, memory loss, bloating, indigestion, vomiting, and diarrhea.

Normal alcohol metabolism is absorbed from the stomach or small intestine when alcohol is ingested, 10% of which is excreted in breath, sweat, and urine, and the remaining 90% of alcohol enters blood vessels and is metabolized in the liver (M. Nakanishi ; Saishin Igaku, 31, p2086, 1976). Subsequently, alcohol dehydrogenase (ADH) present in the liver cells oxidizes the alcohol to acetaldehyde, and acetaldehyde is broken down into acetic acid by acetaldehyde dehydrogenase in the liver cells and transferred to the muscles or adipose tissue of the whole body. Finally, it is decomposed into carbon dioxide gas and water. However, in this decomposition metabolic process, heartburn occurs due to excessive drinking, abnormal alcohol metabolism occurs, and as the alcohol concentration in the blood increases, cerebral blood vessel contraction occurs due to unproduced ethyl alcohol or acetaldehyde, resulting in headache and dizziness.

The hangover reliever has various effects such as thirst, systemic boredom, fatigue, memory loss, abdominal distension, indigestion, vomiting, diarrhea, etc., which can result from excessive drinking. Development seems to be needed.

However, since most hangover-relieving products are developed from herbal ingredients, there is a strong sense of odor and odor, so research on this is necessary. Reduction methods such as herbal medicine and odor may include biological methods such as enzyme treatment, chemical methods such as masking with fragrances, and physical methods such as warming.

Examples of physical methods include cold aged cheonnyeonmokran extract (Republic of Korea Patent No. 10-1948661), maturing rate or its extract (Republic of Korea Patent Registration No. 10-1881369), yanggang using warm ripened jujube (Korea Patent Publication No. 10-2015-0075838), a drink agent containing red ginseng and antler extract containing red ginseng and deer antler extracts that have been subjected to an aging step by leaving them for 8 to 14 hours as they are in a boiling water bath and a method of manufacturing the same (Korea Patent Registration No. 10-1090284) ), red ginseng extract composition change by heat treatment (Agrochemical Journal 24(1): 51-58, 1980), color change of red ginseng extract by heat treatment (Korea Ginseng Society 4(2):50-58, 1981). .

In addition, for the hangover cancellation, the Republic of Korea Patent No. 10-0552398 (a beverage composition for hangover cancellation containing pea extract), Republic of Korea Patent No. 10-0989869 (hangover cancellation beverage containing fermented soybean sprouts), Korea Registered Patent No. 10-1601947 (a hangover-relieving beverage composition containing peanut sprout extract and turmeric extract), Republic of Korea Patent Publication No. 10-2002-0081995 (hangover-relieving by blended foods and liver function improving agent and its manufacturing method), Republic of Korea Patent No. 10-2002-0064151 (low-alcohol insoluble extract fraction with hepatotoxicity and hangover-removing activity isolated from huts and polysaccharide substances and compositions containing the same), including rice bran extract (Gurume), alder, and oak grasses , Originated from various plants, such as galaxies and milk thistle extract, have been reported to be effective in hangovers. However, it is true that these products have insufficient objective verification of the efficacy of the final product, which is actually made, and are not highly reliable for consumers who need a product to cure hangovers. Accordingly, it is urgently needed to develop products that can help consumers to have a healthy life by trusting and reducing social losses due to excessive drinking by verifying efficacy of hangover relief.

Under such a background, the present inventors tried to develop a method that can more effectively relieve hangovers. As a result, a mixture of insect enzyme treatments and herbal extracts effectively reduces the level of alcohol concentration in the blood and is effective in relieving hangovers as well as liver. The present invention was completed by confirming that there is a protective effect.

Republic of Korea Registered Patent No. 10-0477957 (Invention name: New pyrrole derivative having liver protective activity separated from wolfberry and composition containing the same, Applicant: Seoul National University Industry-Academic Cooperation Foundation, Registration Date: March 10, 2005) Republic of Korea Registered Patent No. 10-0633851 (Invention name: Composition for prevention and treatment of liver disease or liver disease containing heat-dried acid garlic extract as an active ingredient, Applicant: School Corporation Sangji Academy, Registration Date: October 2006 April 04) Republic of Korea Registered Patent No. 10-1106499 (Invention name: Food composition for hepatoprotective effect including the extract of young tree bran, Applicant: Jangheung-gun, Registration date: January 10, 2012) Republic of Korea Registered Patent No. 10-1852840 (Invention name: Composition for liver protection containing slug enzyme treatment, Applicant: Hanmi Corporation, Registration Date: April 23, 2018) Republic of Korea Registered Patent No. 10-1919839 (Invention name: Composition for liver protection or hangover elimination containing cricket enzyme treatment, Applicant: Hanmi Corporation, Registration Date: November 13, 2018) Republic of Korea Registered Patent No. 10-0453575 (Invention name: Cricket itself or liver extract containing its extract, tonic fatigue recovery, alcohol metabolism promotion, food composition for inhibiting lipid peroxide production, Applicant: Republic of Korea, Registration Date: October 2004 May 11) Republic of Korea Patent Publication No. 10-2002-0081995 (Invention name: Relieving hangover by blended foods and liver function improving agent and its manufacturing method, Applicant: Dear Co., Ltd., Publication date: October 30, 2002) Republic of Korea Patent Publication No. 10-2002-0064151 (Invention name: low-alcohol insoluble extract fraction and polysaccharide substance having hepatotoxicity and hangover-removing activity isolated from hut tree, and composition containing the same, Applicant: Tree of Life, Ltd. , Release date: August 7, 2002) Republic of Korea Registered Patent No. 10-1948661 (Invention name: Cosmetic composition for skin whitening, which contains low-temperature aged Cheonnyeonmolan extract using bamboo charcoal as an active ingredient, Applicant: Koreana Cosmetics Co., Ltd., Registration Date: February 11, 2019 ) Republic of Korea Registered Patent No. 10-1881369 (Invention name: Antioxidant aging rate skin and antioxidant composition comprising aging rate skin or extracts thereof as an active ingredient, Applicant: Chungnam National University Industry-Academic Cooperation Foundation, Registration Date: July 18, 2018) Republic of Korea Patent Publication No. 10-2015-0075838 (Invention name: yanggang using warm aging jujube and its manufacturing method, Applicant: Kwon Oh-sung, Publication date: July 6, 2015) Republic of Korea Registered Patent No. 10-1090284 (Invention name: Drink agent containing red ginseng and antler extract and manufacturing method thereof, Applicant: Jin Kim, Registration date: November 30, 2011)

An object of the present invention is to provide a composition for resolving hangover or protecting the liver containing an edible insect enzyme treatment and a herbal extract, more specifically, a brown larva larva enzyme treatment, a white spotted flower larva larva enzyme treatment, a cricket adult treatment Water, old pumpkin extract, cinnamon extract, dermis extract, astragalus extract, germinated frost extract, maca extract, ginseng torpedo extract, grape extract, to provide a composition for relieving hangover or protecting liver containing golden extract.

The present invention is a brown larva larvae enzymatic treatment, white spotted flower radish larvae enzymatic treatment, cricket adult enzymatic treatment, old pumpkin extract, cinnamon extract, dermis extract, astragalus extract, germinated frost extract, maca extract, ginseng lightning extract, grapes The present invention relates to a composition for relieving hangover or protecting the liver containing an extract and a golden extract.

More preferably, the composition is based on 100 parts by weight of the brown larva larvae enzyme treatment, 20 to 40 parts by weight of white spotted flower larvae larvae enzyme treatment, 20 to 40 parts by weight of cricket adult enzyme treatment, 50 to 80 years old pumpkin extract Parts by weight, cinnamon extract 30-110 parts by weight, dermis extract 70-150 parts by weight, astragalus extract 30-100 parts by weight, germination frost extract 120-150 parts by weight, maca extract 40-90 parts by weight, ginseng lightning extract 100-150 It may contain parts by weight, 30 to 100 parts by weight of grape extract, and 2 to 50 parts by weight of golden extract. At this time, if the mixture of enzyme treatments or extracts of each insect deviates from the mixing ratio, it is not preferable because it can reduce hangover or reduce liver protection.

In addition, the composition includes baekgae fruit extract, turmeric extract, root extract, thistle extract, pogongyoung extract, mugwort extract, apple extract, licorice extract, jujube extract, aspartic acid, liquid fructose, citric anhydride, vitamin C, taurine, calcium lactate, L -Arginine, trisodium citrate, nicotinic acid amide, enzymatic treatment stevia, vitamin B1 hydrochloride, vitamin B2 and DL-alpha-tocopheryl acetate may be further included, and an appropriate amount of purified water may be contained.

More preferably,

Based on 100 parts by weight of the enzyme treatment of brown larvae, 250 to 400 parts by weight of oyster fruit extract, 50 to 100 parts by weight of turmeric extract, 50 to 100 parts by weight of extract of brown root, 50 to 100 parts by weight of thistle extract, 50 to 100 parts of pogongyoung extract Parts by weight, mugwort extract 50-100 parts by weight, apple extract 200-300 parts by weight, licorice extract 5-20 parts by weight, jujube extract 5-20 parts by weight, aspartic acid 50-100 parts by weight, liquid fructose 3000-4000 parts by weight, 100 to 200 parts by weight of citric anhydride, 30 to 70 parts by weight of vitamin C, 20 to 50 parts by weight of taurine, 20 to 40 parts by weight of calcium lactate, 10 to 30 parts by weight of L-arginine, 5 to 20 parts by weight of trisodium citrate, nicotinic acid It is preferable that 5 to 20 parts by weight of amide, 1 to 5 parts by weight of enzyme-treated stevia, 1 to 3 parts by weight of vitamin B1 hydrochloride, 1 to 3 parts by weight of vitamin B2 and 1 to 3 parts by weight of DL-alpha-tocopheryl acetate are included.

The composition may contain an appropriate amount of purified water (water), based on 100 parts by weight of the brown larva larvae enzyme treatment, may contain 20000 to 50000 parts by weight of purified water.

Of the extract raw materials used in the composition of the present invention, germinated bean extract may be selected from soybean sprout cotyledons, soybean germinated pears and soybean germinated roots, and may also be germinated soybean cotyledons and germinated bean pears and whole germinated bean roots. .

The present invention provides a pharmaceutical composition and a health functional food for preventing, improving or treating alcoholic liver disease containing the composition. The alcoholic liver disease may be alcoholic hepatitis, alcoholic fatty liver or alcoholic cirrhosis.

The edible insect enzyme treatment used in the present invention may be a raw material insect treated with an enzyme selected from flavozyme, alkylase, or protease N. It is most preferred.

The edible insect enzyme treatment is added to 200 to 400 parts by weight of purified water based on 100 parts by weight of the raw edible insect, and then heated at 100 to 150°C for 0.5 to 2 hours, cooled to 40 to 60°C, and then for 5 to 10 hours. It may be enzymatically treated. At this time, it is preferable to add 0.1 to 10 parts by weight of enzyme based on 100 parts by weight of the raw material edible insect.

In addition, after the enzymatic treatment, it can be concentrated to a sugar content of 20 to 70 Brix and powdered as needed, and the enzyme can be deactivated by heat treatment at 90 to 100° C. for 20 to 60 minutes before or after concentration. have.

Alternatively, the liquid obtained by concentrating and filtering the enzyme-treated can be dried, and immediately after or after the enzymatic treatment, the oil component separated from the edible insect of each raw material can be separated by layer separation.

The liquid phase immediately after the enzymatic treatment or after concentration can be dried and powdered, and conventional drying methods such as spray drying, hot air drying, and freeze drying can be used.

During the enzyme treatment as described above, if the purified water is less than 200 parts by weight based on 100 parts by weight of the raw material edible insect, the enzyme may be overactivated in the subsequent enzymatic treatment process, and the quality of the proteolytic composition may deteriorate, and the purified water may contain 400 parts by weight. If exceeded, the enzyme activity is weak during subsequent enzymatic treatment, and proteolysis may not be performed well.

The heating conditions serve to simultaneously perform the sterilization process and the conditions for ripening insects. If the heating temperature is less than 100°C, sterilization may not occur well, and when the heating temperature exceeds 150°C, the property of the protein changes. It appears that the quality of the proteinized composition may deteriorate. Likewise, even if the heating condition is less than 0.5 hours, sterilization may not be performed well, and when the heating time exceeds 2 hours, a property of the protein may change.

When the enzyme treatment temperature is less than 40°C, enzymatic activity may hardly appear, and when it exceeds 60°C, the enzyme may be deactivated and may not function.

In addition, in the process of enzyme treatment, when the enzyme is added in an amount of less than 0.1 parts by weight based on 100 parts by weight of the raw material edible insect, the enzyme activity may hardly appear, and the addition of more than 10 parts by weight is in terms of the cost of using the enzyme. It is undesirable and the action of the enzyme is excessive and the properties of the final proteolytic product may be worse.

At this time, the raw material edible insect is used to fast and discharge the feces completely, but after washing with purified water in a raw state, it is recommended to add purified water and heat it.After the heating process is sufficiently performed, the edible insect is cooled and then the edible insect It is preferred to perform a hydrolysis process by crushing and enzymatic treatment.

Each extract used in the present invention can be extracted using water, C1 to C4 alcohol or a mixed solution thereof as a solvent in the raw material sample for preparing the extract, and the C1 to C4 alcohol is methanol, ethanol, propanol, isopropanol, butanol and It can be selected from the group consisting of isobutanol. The solvent is preferably 50 to 70 (v/v)% aqueous solution of water or ethanol.

In addition, water, C1 to C4 alcohol or a mixed solution thereof used in the preparation of the extract may be used in a volume of 1 to 40 times (1 to 40 liters per 1 kg) or 1 to 40 times weight based on the weight of the raw material sample used, preferably 5 to 10 times the volume or 5 to 10 times the weight can be used.

Extraction conditions of the extract may be from 1 minute to 48 hours at 20 ~ 100 ℃. The above process can be repeated 1 to 4 times. More preferably, after extracting for 5 to 8 hours at a temperature of 80 to 95°C (heating process), it is better to collect only the extract liquid phase and then agitate for 8 to 12 hours at 50 to 70°C (aging process). Thereafter, the extract can be concentrated under reduced pressure at 50 to 60°C to prepare the extract in a concentrated state of 20 to 70 brix and powdered as necessary.

In addition, the present invention provides a pharmaceutical composition for the prevention or treatment of alcoholic liver disease containing the composition. The pharmaceutical composition may be formulated and used in the form of oral dosage forms, external preparations, suppositories, and sterile injectable solutions, such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, etc., according to a conventional method. Carriers, excipients and diluents that can be included in the pharmaceutical composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose , Methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. In the case of formulation, it is prepared using diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents and surfactants. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc. These solid preparations include at least one excipient in the pharmaceutical composition of the present invention, for example, starch, calcium carbonate, sucrose or lactose. It is prepared by mixing gelatin, etc. In addition, lubricants such as magnesium stearate and talc are used in addition to simple excipients. Liquid preparations for oral use include suspensions, intravenous solutions, emulsions, syrups, etc. In addition to water and liquid paraffin, which are commonly used diluents, various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, can be included. . Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, and suppositories. Non-aqueous solvents and suspensions may include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate. As a base for suppositories, witepsol, macrogol, tween 61, cacao butter, laurin butter, and glycerogelatin may be used.

The dosage of the pharmaceutical composition of the present invention will vary depending on the age, gender, weight of the subject to be treated, the specific disease or pathology to be treated, the severity of the disease or pathology, the route of administration, and the judgment of the prescriber. Dosage determination based on these factors is within the level of those skilled in the art, and dosages generally range from 0.01 mg/kg/day to approximately 2000 mg/kg/day. A more preferred dosage is 1 mg/kg/day to 500 mg/kg/day. The administration may be administered once a day, or may be divided into several times. The above dosage does not limit the scope of the present invention in any way.

The pharmaceutical composition of the present invention can be administered to various mammals, such as rats, livestock, and humans. All modes of administration can be expected, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intrauterine dura mater or intracranial injection.

In addition, the present invention provides a health functional food for the prevention or improvement of alcoholic liver disease, which contains a composition comprising a food additive that is food-acceptable. The health functional food of the present invention includes tablets, capsules, pills or liquids, etc. As foods to which the composition of the present invention can be added, for example, various drinks, meat, sausage, bread, candy, There are snacks, noodles, ice cream, dairy products, soups, drinks, alcoholic beverages, chewing gum, tea and vitamin complexes.

The present invention is a brown larva larvae enzymatic treatment, white spotted flower radish larvae enzymatic treatment, cricket adult enzymatic treatment, old pumpkin extract, cinnamon extract, dermis extract, astragalus extract, germinated frost extract, maca extract, ginseng lightning extract, grapes It relates to a composition containing an extract and a golden extract, excellent in resolving hangover due to increased activity of alcohol-degrading enzymes, and hepatic toxicity induced by alcohol is alleviated to treat various diseases related to liver damage such as alcoholic hepatitis and alcoholic fatty liver. Or it can be easily used as a health functional food for the prevention and improvement of these diseases.

Hereinafter, a preferred embodiment of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. Rather, it is provided to sufficiently convey the spirit of the present invention to those skilled in the art so that the contents introduced herein are thorough and complete.

Preparation Example 1. Preparation of enzyme treatment products and herbal extracts and preparation of additives

Production Example 1-1. Preparation of enzyme treatment of brown larva larvae, enzyme treatment of white spotted flower larvae, and enzyme treatment of double-headed cricket

In order to prepare a composition for relieving hangover, an enzyme treatment product of a brown larva larvae, an enzyme treatment product of a white spotted flower larvae, and an enzyme treatment product of a paired cricket were prepared.

Eight brown larvae larvae, white spotted larvae larvae raised for 35 days, and double-headed cricket adults of 2.5~3.0cm in size, respectively, were purchased from specialized breeding farms. For brown larvae, fasting for three days, white spotted larvae larvae Silver was fasted for 3 days to discharge the feces first, and then glutinous rice flour was fed for 7 days, then fasted for 2 days, and Ssangbye cricket was fasted for 1 day, and washed three times with running water in a state where the feces were completely discharged.

after. After putting brown larvae, white-spotted flower larvae, and double-headed cricket larvae into the steamer, respectively, and adding 3 times the weight of purified water to add a pressure of 1.5 kgf/cm ² and The mixture was heated for 90 minutes at a pressurized high temperature condition of 121°C, and then pulverized under conditions of bead size 0.4 mm and rotor speed 3,000 rpm using a wet grinder (LSI, NEYZSCH, Srlb Bavaria, Germany) in the state containing water. Grinding products are prepared for brown larvae, white spotted larvae larvae, and double-starred cricket adults. Thereafter, 1 g of Alcalase (Subtilisin Carlsberg, Novozymes) per 100 g of each pulverized product was added to perform hydrolysis at 40° C. for 5 hours. Each hydrolyzed product was filtered and concentrated to 60 brix under a reduced pressure concentrator under conditions of 60° C. to obtain a brown larva larvae enzyme treatment, a white spotted flower larvae enzyme treatment, and a paired cricket enzyme treatment.

Preparation Example 1-2. Preparation of old pumpkin extract, cinnamon extract, dermis extract, astragalus extract, germinated frost extract, maca extract, ginseng torpedo extract, grape extract and golden extract

The herbal extracts for the composition for the hangover relief were prepared by using old pumpkin, cinnamon, dermis, astragalus, germinated frost, ginseng torpedo, grapes and gold as the extraction raw materials. At this time, Seotae used to have 1~2cm buds after soaking in water.

To this end, each of the extracted raw materials was added with an aqueous solution of 75 (v/v)% ethanol or water of 8 times the weight, extracted at 80° C. for 12 hours and filtered to obtain only a liquid phase. After the collected liquid was put into a double jacket with temperature control, and then aged for 10 hours at 70°C, the mixture was concentrated to 60 brix using a reduced pressure concentrator at 60°C to obtain each extract. At this time, the old pumpkin and the dermis were prepared by using 75(v/v)% ethanol aqueous solution as a solvent and the rest as water as a solvent.

In addition, maca extract was prepared by purchasing a maca concentrate of 65 brix from Chile extracted with an aqueous 75(v/v)% ethanol solution.

Preparation Example 1-3. Preparation of additives

As an additional additive for the production of a composition for relieving hangovers, buckthorn fruit extract, turmeric extract, brown root extract, thistle extract, pogongyoung extract, mugwort extract, apple extract, licorice extract, jujube extract, aspartic acid, liquid fructose, citric anhydride, vitamin C, taurine , Calcium lactate, L-arginine, trisodium citrate, nicotinamide, enzymatic treatment stevia, vitamin B1 hydrochloride, vitamin B2 and DL-alpha-tocopheryl acetate were prepared. At this time, each extract was prepared by the method of Preparation Example 1-2, but extracts of buckthorn, turmeric, brown root, thistle, pogongyoung, and wormwood were prepared using 75(v/v)% ethanol aqueous solution as a solvent, apple, licorice , The extract of jujube was prepared using water as a solvent. Enzyme-treated stevia (stevia extract) is a sugar substitute sweetener that is commonly used and was purchased from Daepyeong Co., Ltd. and used.

<Example/Comparative Example. Preparation of hangover-relieving composition>

The following hangover relief composition was prepared by mixing the sample prepared in Preparation Example 1.

Examples 1-3

Each sample was mixed under the conditions of Table 1 below, and water was added to prepare a beverage composition having a total weight of 3 t.

sample Example 1 Example 2 Example 3 Example 4 Enzyme treatment of brown larvae (g) 100 100 100 100 White Spotted Flower Plain Larva Enzyme Treatment (g) 20 30 40 30 Twin-Star Cricket Enzyme Treatment (g) 40 30 20 30 Old Pumpkin Extract (g) 80 80 50 65 Cinnamon extract (g) 110 30 80 70 Dermis extract (g) 100 70 150 110 Astragalus extract (g) 30 100 100 65 Germinated frost extract (g) 150 140 120 135 Maca extract (g) 80 40 90 65 Ginseng torpedo extract (g) 100 150 100 125 Grape extract (g) 70 100 30 65 Golden extract (g) 40 50 40 26

Example 5

The composition of Example 1 was mixed with additives such as buckthorn extract under the following conditions, and water was added to prepare a beverage composition to have a total weight of 3 tons.

Ingredients of Example 1: brown larva larvae enzymatic treatment 100 g, white spotted flower larvae larvae enzymatic treatment 20 g, double-headed cricket adult enzymatic treatment 40 g, old pumpkin extract 80 g, cinnamon extract 110 g, dermis extract 100 g , Hwanggi extract 30 g, germinated frost extract 150 g, maca extract 80 g, ginseng torpedo extract 100 g, grape extract 70 g, golden extract 40 g

Additional Ingredients: 325 g of buckthorn extract, 75 g of turmeric extract, 75 g of extract of thistle root, 75 g of thistle extract, 75 g of pogongyoung extract, 75 g of mugwort extract, 250 g of apple extract, 12 g of licorice extract, 12 g of jujube extract, 12 g of aspartic acid 75 g, liquid fructose 3500 g, citric anhydride 150 g, vitamin C 50 g, taurine 35 g, calcium lactate 30 g, L-arginine 25 g, trisodium citrate 12 g, nicotinic acid amide 12 g, enzyme-treated stevia 3 g, 2 g of vitamin B1 hydrochloride, 2 g of vitamin B2, 2 g of DL-alpha-tocopheryl acetate

Example 6

The composition of Example 4 was mixed with additives such as buckthorn extract under the following conditions, and water was added to prepare a beverage composition to have a total weight of 3 tons.

Ingredients of Example 4: brown larva larvae enzyme treatment 100 g, white spotted flower larvae larvae enzyme treatment 30 g, double-headed cricket adult enzyme treatment 30 g, old pumpkin extract 65 g, cinnamon extract 70 g, dermis extract 110 g , Hwanggi extract 65 g, germinated frost extract 135 g, maca extract 65 g, ginseng torpedo extract 125 g, grape extract 65 g, golden extract 26 g

Additional Ingredients: 325 g of buckthorn extract, 75 g of turmeric extract, 75 g of extract of thistle root, 75 g of thistle extract, 75 g of pogongyoung extract, 75 g of mugwort extract, 250 g of apple extract, 12 g of licorice extract, 12 g of jujube extract, 12 g of aspartic acid 75 g, liquid fructose 3500 g, citric anhydride 150 g, vitamin C 50 g, taurine 35 g, calcium lactate 30 g, L-arginine 25 g, trisodium citrate 12 g, nicotinic acid amide 12 g, enzyme-treated stevia 3 g, 2 g of vitamin B1 hydrochloride, 2 g of vitamin B2, 2 g of DL-alpha-tocopheryl acetate

Comparative Examples 1 to 3

Each sample was mixed under the conditions of Table 2 below, and water was added to prepare a beverage composition having a total weight of 3 t.

sample Comparative Example 1 Comparative Example 2 Comparative Example 3 Enzyme treatment of brown larvae (g) 400 0 100 White Spotted Flower Plain Larva Enzyme Treatment (g) 300 0 150 Twin-Star Cricket Enzyme Treatment (g) 220 0 69 Old Pumpkin Extract (g) 0 70 100 Cinnamon extract (g) 0 110 10 Dermis extract (g) 0 150 30 Astragalus extract (g) 0 100 210 Germinated frost extract (g) 0 120 40 Maca extract (g) 0 90 20 Ginseng torpedo extract (g) 0 150 180 Grape extract (g) 0 70 10 Golden extract (g) 0 60 One

Comparative Example 4

A total of 920 g of the composition of Example 2-1 of Korean Patent Registration No. 10-1919839 was prepared, and water was added to make a total of 3 tons.

Comparative Example 5

Prepare the composition of Example 2-1 of Republic of Korea Patent No. 10-1852840 No. 920g and added water to make a total of 3t.

Comparative Example 6

To the components of Comparative Example 3 was mixed with additives such as buckthorn extract under the following conditions and water was added to prepare a beverage composition to have a total weight of 3 tons.

Components of Comparative Example 3: brown larva larvae enzymatic treatment 100 g, white spotted flower larvae larvae enzymatic treatment 150 g, double-starred cricket adult enzyme treatment 69 g, old pumpkin extract 100 g, cinnamon extract 10 g, dermis extract 30 g , Hwanggi extract 210 g, germinated frost extract 40 g, maca extract 20 g, ginseng torpedo extract 180 g, grape extract 10 g, golden extract 1 g

Additional Ingredients: 325 g of buckthorn extract, 75 g of turmeric extract, 75 g of extract of thistle root, 75 g of thistle extract, 75 g of pogongyoung extract, 75 g of mugwort extract, 250 g of apple extract, 12 g of licorice extract, 12 g of jujube extract, 12 g of aspartic acid 75 g, liquid fructose 3500 g, citric anhydride 150 g, vitamin C 50 g, taurine 35 g, calcium lactate 30 g, L-arginine 25 g, trisodium citrate 12 g, nicotinic acid amide 12 g, enzyme-treated stevia 3 g, 2 g of vitamin B1 hydrochloride, 2 g of vitamin B2, 2 g of DL-alpha-tocopheryl acetate

Experimental Example 1. Sensory test of beverage composition

The sensory evaluation was performed on the beverage compositions of Example 5, Example 6 and Comparative Example 6. The sensory evaluation involved 10 experts and 20 randomly selected non-professionals.

The preference test used in the evaluation method is a 7-point scale method, and I hate it for 1 point. I hate 2 points. I hate 3 points. 4 points is normal. Five points is good. 6 is good. We proceeded by classifying with 7 points very good. As a result, as shown in Table 3, the beverage compositions of Example 5 and Example 6 showed that the color, taste, odor, and taste were all increased compared to the beverage composition of Comparative Example 6.

division color flavor No odor Preference Example 5 6.4 6.0 6.5 5.9 Example 6 5.8 5.9 6.2 6.0 Comparative Example 6 4.3 4.1 3.9 4.2

Experimental Example 2. Confirmation of the activity of alcohol degrading enzyme

The activity of alcohol degrading enzymes, aldehyde dehydrogenase (ALDH) and acetaldehyde (ADH), was measured.

To this end, each composition of Examples 1 to 4 and Comparative Examples 1 to 5 before adding water to prepare a beverage was diluted in dimethyl sulfoxide (DMSO) so that each component was evenly mixed and prepared at a concentration of 100 mg/ml. Each experiment was performed using this.

The activity of ALDH was measured using the Aldehyde dehydrogenase activity colorimetric assay kit (BioVision, catalog #K731-100) according to the BioVision protocol. At this time, the sample was not added as a control, and the ALDH activity of the sample was expressed as a relative activity (%) relative to the control. After measuring three times to obtain the average value, it is shown in Table 4 below.

ADH activity was measured according to the BioVision protocol using an alcohol dehydrogenase activity colorimetric assay kit (BioVision, catalog #K787-100). At this time, the sample was not added as a control, and the ADH activity of the sample was expressed in relative activity (%) relative to the control, and is shown in Table 4 below.

division ALDH activity (%) ADL activity (%) Control 100 100 Example 1 176 350 Example 2 165 363 Example 3 158 312 Example 4 161 314 Comparative Example 1 115 152 Comparative Example 2 107 179 Comparative Example 3 117 162 Comparative Example 4 132 215 Comparative Example 5 122 256

Experimental Example 3. Confirmation of the liver cell protective activity of the mixture (in vitro)

Prepared in Experimental Example 2 The DMSO dilutions of Examples 1 to 4 and Comparative Examples 1 to 5 were treated with ethanol-treated human liver cancer cell line (HepG2) to confirm the protective activity of liver cells damaged by alcohol.

At this time, HepG2 was cultured in the following manner. Specifically, 10% FBS (fetal bovine serum) and 100 IU/mL penicillin-added DMEM (Dulbecco's modified Eagle's medium, Giboc, USA) medium were used as a culture medium and cultured in an incubator at 37°C and 5% CO ₂ conditions. , Passed once every 3-4 days.

For the experiment, HepG2 was incubated in a plate at a concentration of 7x10 ³ /well for 24 hours, and each diluted sample was treated to a final concentration of 100 µg/ml. After 1 hour, 95% ethanol was added so that each sample was 200 mM in the treated medium, and then cultured for 17 hours. Cells were induced by replacing the medium every day under the same conditions for 3 days and treating each sample and ethanol by the method. Then, the cell viability was investigated by measuring absorbance at 485 nm using the MTT assay method. The cell viability was calculated based on 100% of the untreated (control) survival rate.

division Cell viability (%) Control 100.2 ethanol 25.2 Ethanol + Example 1 96.4 Ethanol + Example 2 95.2 Ethanol + Example 3 98.2 Ethanol + Example 4 97.5 Ethanol + Comparative Example 1 48.2 Ethanol + Comparative Example 2 45.2 Ethanol + Comparative Example 3 48.2 Ethanol + Comparative Example 4 67.5 Ethanol + Comparative Example 5 69.2

As a result, as shown in Table 5, it can be confirmed that the survival rate of HepG2 was significantly higher in the diluted sample treatment groups of Examples 1 to 4 compared to the diluted sample treatment groups of Comparative Examples 1 to 5, and most of the damages caused by alcohol treatment It can be seen to recover.

Experimental Example 4. Evaluation of hangover cancellation

The effect of releasing hangover on the beverage compositions of Examples 5, 6 and 6 was evaluated.

At this time, the following two products sold as hangover-relieving beverages were also used.

#1: Rice embryo fermentation extract, taurine, hut fruit extract, ginger, yeast extract, Zara extract, astragalus extract, citric anhydride, DL-apple acid, D-alanine, nicotinic acid amide, trisodium citrate, complex herbal extract, synthetic flavor , Fructose, sugar

#2: Hwangchil tree extract, apple extract, taurine, quince extract, citric acid, vitamin C, golden complex extract, enzymatic treatment stevia, L-asparagine, fructose, fructooligosaccharide, honey

The evaluation method is as follows. The test subjects were adult men and women who drink 1 bottle of soju (360 ml) 2 to 3 times a week, and were randomly evaluated by collecting 10 of them into each treatment group.

The drinking method was to drink 1 point of pork belly as a snack in a glass of soju with an alcohol content of 19% (v/v), drink 1.5 bottles (360 ml) per person for 30 minutes, and fever after 3 hours after drinking. The inhibitory effect on thirst, odor, and headache was evaluated by a 5-point evaluation method.

5: Very good <-> 1: Very bad

As a result of the evaluation, as shown in Table 6, the beverage composition of the present invention was evaluated as having an excellent effect of suppressing fever, thirst, odor, headache, nausea, and vomiting.

division Fever suppression Suppress thirst Suppression Headache suppression Suppressing nausea Suppressing vomiting Example 5 4.3 4.4 4.3 4.6 4.1 4.2 Example 6 4.5 4.2 4.5 4.2 4.2 3.9 Comparative Example 6 3.1 3.1 3.2 3.4 3.3 3.2 #One 2.8 3.2 3.3 2.7 3.5 2.9 #2 3.6 2.9 3.6 2.7 3.2 2.8

Claims (4)

Based on 100 parts by weight of the brown larva larvae enzyme treatment, 20 to 40 parts by weight of the white spotted flower larvae larvae enzyme treatment, 20 to 40 parts by weight of cricket adult enzyme treatment, 50 to 80 parts by weight of the old pumpkin extract, and 30 to cinnamon extract 110 parts by weight, dermis extract 70-150 parts by weight, astragalus extract 30-100 parts by weight, germinated frost extract 120-150 parts by weight, maca extract 40-90 parts by weight, ginseng hoehwa extract 100-150 parts by weight, grape extract 30- Health functional food for hangover relief or liver protection, characterized by containing 100 parts by weight and 2 to 50 parts by weight of golden extract. According to claim 1,
In the above foods, fruit extract, turmeric extract, root extract, thistle extract, pogongyoung extract, mugwort extract, apple extract, licorice extract, jujube extract, aspartic acid, liquid fructose, citric anhydride, vitamin C, taurine, calcium lactate, L-arginine , Trisodium citrate, nicotinic acid amide, enzymatic treatment stevia, vitamin B1 hydrochloride, vitamin B2 and DL-alpha-tocopheryl acetate are added to hangover or health functional food for liver protection. Based on 100 parts by weight of the brown larva larvae enzyme treatment, 20 to 40 parts by weight of the white spotted flower larvae larvae enzyme treatment, 20 to 40 parts by weight of cricket adult enzyme treatment, 50 to 80 parts by weight of the old pumpkin extract, and 30 to cinnamon extract 110 parts by weight, dermis extract 70-150 parts by weight, astragalus extract 30-100 parts by weight, germinated frost extract 120-150 parts by weight, maca extract 40-90 parts by weight, ginseng hoehwa extract 100-150 parts by weight, grape extract 30- Contains a composition containing 100 parts by weight and 2 to 50 parts by weight of golden extract, or
In the above composition, fruit extract, turmeric extract, brown root extract, thistle extract, pogongyoung extract, mugwort extract, apple extract, licorice extract, jujube extract, aspartic acid, liquid fructose, citric anhydride, vitamin C, taurine, calcium lactate, L-arginine , Trisodium citrate, nicotinic acid amide, enzymatic treatment stevia, vitamin B1 hydrochloride, vitamin B2 and DL-alpha-tocopheryl acetate.
Pharmaceutical composition for the prevention or treatment of alcoholic liver disease. A health functional food for the prevention or improvement of alcoholic liver disease, comprising the food of claim 1 or 2.