KR100673594B1 - Extracts of soy hypocotyl and/or hull with inhibitory effects on carcinogenesis and composition comprising the same - Google Patents

Abstract

The present invention relates to a soybean embryo and / or soybean bark extract having cancer cell proliferation inhibitory effect and a composition for inhibiting cancer comprising the same as an active ingredient, wherein the solvent extract of soybean can be safely ingested without a separate purification process. In addition, cancer can be effectively suppressed, and it can be usefully used for a composition for inhibiting cancer, processed food, functional food, food additives, etc., and at the same time, economic efficiency can be improved by recycling soybean hulls.

Soybean embryo, soybean hull, liver cancer, stomach cancer, breast cancer, solid cancer, cancer cell proliferation inhibition, food

Description

Soybean embryos and / or soybean husk extracts, and compositions for inhibiting carcinogenesis comprising the same

1 is a graph measuring the efficacy of ethanol extracts of soybean embryos and soybean hulls according to the present invention inhibit the proliferation of liver cancer cells.

Figure 2 is a graph measuring the efficacy of inhibiting the proliferation of liver cancer cells hot water extract of soybean embryos and soybean hulls according to the present invention.

Figure 3 is a graph measuring the efficacy of ethanol extracts of soybean embryos and soybean hulls according to the present invention inhibit the proliferation of breast cancer cells.

Figure 4 is a graph measuring the efficacy of inhibiting the proliferation of breast cancer cells hot water extract of soybean embryos and soybean hulls according to the present invention.

5 is a graph measuring the efficacy of inhibiting the proliferation of gastric cancer cells ethanol extract of soybean embryo and soybean peel according to the present invention.

Figure 6 is a graph measuring the efficacy of inhibiting the proliferation of gastric cancer cells hot water extract of soybean embryos and soybean shell according to the present invention.

7 is a graph measuring the efficacy of ethanol extracts of soybean embryos and soybean hulls according to the present invention inhibit the proliferation of solid cancer cells.

Figure 8 is a graph measuring the efficacy of the hot water extract of soybean embryos and soybean hulls according to the present invention inhibit the proliferation of solid cancer cells.

The present invention relates to a solvent extract of soybean embryos and / or soybean hulls having an effect of inhibiting the proliferation of various cancer cells, and a composition for inhibiting carcinogenesis comprising the same as an active ingredient.

Although surgery, radiotherapy, and chemotherapy have been used for decades, the incidence and mortality of most epithelial cancers, including liver cancer, breast cancer, and gastric cancer, has not decreased substantially [Sporn, MB, Lancet, 347]. : 1377-1381, 1996. This is because, over the past 30 years, cancer has been studied by a therapeutic approach rather than a preventive approach. Thus, as already known, eating ingredients or foods that can effectively inhibit or slow the multi-stage outbreak of cancer that lasts for decades can have more substantial results in lowering cancer incidence and mortality. [Kang Jin-suk et al., Chemical Cancer Prevention, Korea Medicine, 2000; Surh, Mutat. Res., 428: 305-327, 1998; Sporn, M. B., Lancet, 347: 1377-1381, 1996].

Since cancer cells or transformed cells have a much faster and infinitely proliferative characteristic than normal cells, the proliferation inhibitory effect of cancer cells has been recognized to have the effect of preventing and inhibiting cancer [Kang, Jin-seok et al., Chemical cancer prevention, Korea Medicine, 135-136 , 2000; Yamasaki, H. et al, Carcinogenesis 11: 1051-1058, 1999; Kelloff. G. J. et al., Eur. J. Cancer, 35: 1755-1762, 1999; Surh, Y. J., Mutat. Res, 428: 305-327, 1999; Trosko, J. E., Cancer Res. 53, 3475-3485 (1993).

On the other hand, soybeans, one of the traditional foods in our country, play an important part in our diet. Soybean is generally used as a source of high-quality protein and lipids, and has been used for various purposes from fermented foods such as miso and soy sauce, and non-fermented foods such as tofu, bean sprouts, and soy flour. These legumes have recently been in the spotlight as functional foods that can prevent cancer and other adult diseases.

By the way, soybean hulls and embryos that are produced as a by-product of the soybean are almost discarded. According to the US Soybean Association data, about 1.5 million tons of soybeans were imported and used in 2003, resulting in about 45,000 tons of soybean hulls and soybean embryos being discarded by-products.

Cholesterol Inhibitory Effect of Soybean Shell (Kim, Mi-Kin and Moon-Won Song, Young-Song Song, Korean Society of Soybean Research, Vol. 15, No. 1, 23, 1998) Effects and anti-arteriosclerosis effects of soybean embryos (Kang et al., Food and Health Culture, Gwangmungak, 2001) have been reported. However, there have been no reports that the skin and embryo extracts of soybeans used as foods have an excellent effect on cancer cell proliferation than soybeans or halves.

Therefore, there is a need to provide a cancer cell proliferation inhibitor, and a food or drug containing the same, which can be safely ingested without a separate purification process by extracting active ingredients from discarded soybean hulls and embryos.

In order to solve the above problems, the inventors of the present invention, while searching for carcinogenic inhibitors derived from natural foods that ensure human safety, soybean embryos and soybean bark extract exhibits the effect of inhibiting the proliferation of liver cancer, breast cancer, stomach cancer, solid cancer cells The present invention has been completed by discovering that the carcinogenic effect is suppressed.

Accordingly, it is an object of the present invention to provide a solvent extract of a substance selected from the group consisting of soybean embryos, soybean hulls and mixtures thereof which have cancer cell proliferation inhibitory efficacy.

Another object of the present invention is to provide a composition for inhibiting carcinogenesis comprising a solvent extract of a substance selected from the group consisting of soybean embryos, soybean hulls and mixtures thereof having the cancer cell proliferation inhibitory effect as an active ingredient.

In order to achieve the above object, the present invention provides a solvent extract of a substance selected from the group consisting of soybean embryos, soybean hulls, and mixtures thereof, having an inhibitory effect on cancer cell proliferation.

In addition, the present invention provides a composition for inhibiting cancer, comprising a solvent extract of a substance selected from the group consisting of soybean embryos, soybean hulls, and mixtures thereof, having an effect of inhibiting cancer cell proliferation.

Hereinafter, the present invention will be described in detail.

The present invention relates to a solvent extract of a substance selected from the group consisting of soybean embryos, soybean hulls, and mixtures thereof, and a composition for inhibiting cancer cell proliferation comprising the same as an active ingredient.

The solvent extract of a substance selected from the group consisting of soybean embryos, soybean hulls, and mixtures thereof of the present invention exhibits the effect of inhibiting the proliferation of cells such as liver cancer, breast cancer, gastric cancer, or solid cancer, and thus has a carcinogenic effect. It can be safely consumed without purification. In addition, the present invention can provide an economic effect by recycling the discarded soybean hulls, embryos and the like.

The solvent extract, as a water extract or an alcohol extract, may be prepared using conventional extraction methods well known to those skilled in the art. As an example of the preparation method of the solvent extract, a solvent is added to a substance (fraction of soybean) selected from the group consisting of soybean embryos, soybean hulls, and mixtures thereof, and the mixture is extracted under reflux using a stirrer at room temperature. After filtering the extract thus obtained, the solvent is removed and freeze-dried to obtain a solvent extract. As the solvent used to obtain the solvent extract, water, acetone, ethanol, or methanol is preferably used, and in order to apply to food, water or ethanol is more preferred, but is not necessarily limited thereto. In addition, the solvent is preferably 20 to 80% of a solvent such as ethanol, acetone, or methanol, more preferably may be an aqueous solution containing 50% of the solvent.

In addition, the present invention provides a composition for inhibiting cancer, comprising the solvent extract of the soybean embryo and / or soybean shell as an active ingredient, which has cancer cell proliferation inhibitory effect. Cancers to which the compositions of the present invention are applicable include liver cancer, breast cancer, gastric cancer, or solid cancer.

Since the composition of the present invention can be safely ingested without a separate purification process, it can be applied more widely than conventional methods when applied as a food and pharmaceutical composition to ensure stability.

Therefore, the composition of the present invention may be a pharmaceutical composition or a food composition, the food composition in the present invention includes processed foods, functional foods, dietary supplements, food additives, functional beverages and beverage additives and the like.

In the composition of the present invention, water or solvent extracts of soybean embryos and / or soybean hulls are preferably contained in an amount of 0.01 to 50% by weight, more preferably 0.01 to 10% by weight, based on the total composition. At this time, if the content of the solvent extract is less than 0.01% by weight is insufficient carcinogenesis inhibitory effect, if it exceeds 50% by weight it is not economically preferable to increase the amount used.

In addition, the compositions of the present invention may further comprise a pharmaceutically acceptable suitable carrier, excipient or diluent well known to those skilled in the art. Such carriers include saline, buffered saline, water, glycerol and ethanol, and other suitable carriers, excipients and diluents include, but are not limited to, lactose, dextrose, sucrose, sorbitol, mannitol, xyitol, erythritol, maltitol, starch, Acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and Mineral oil and the like. However, the present invention is not limited thereto, and any suitable agent known in the art (Remington's Pharmaceutical Science (Recent Edition), Mack Publishing Company, Easton PA) can all be used.

The composition comprising the extract of soybean embryos and / or soybean hulls having the cancer cell proliferation inhibitory effect as an active ingredient is preferably orally administered, when used as a medicament can be prepared by a method known in the pharmaceutical field, the form As a powder, granules, tablets, capsules, pills, troche, liquid solution, suspension, emulsion, and syrups, or the like can be used.

When using a composition for inhibiting cancer cell proliferation comprising a solvent extract of soybean embryos and / or soybean hulls according to the present invention as food, it is possible to obtain a cancer cell proliferation inhibitory effect by safely ingesting without a separate purification process to remove toxicity. . In addition, it is possible to separate substances that exhibit high cancer cell proliferation inhibitory effect with a simple separation process, thereby lowering the production cost compared to conventional cancer inhibitors, and in particular, by recycling the enormous amount of soybean hulls discarded during soybean processing. Can be improved.

Therefore, when the composition of the present invention is used as a food, a solvent extract selected from the group consisting of soybean embryos, soybean hulls, and mixtures thereof may be prepared as it is, or by mixing with various components used in conventional foods. As the form of the food to be prepared, it can be prepared in all kinds of foods such as solid foods, creamy or jam-like semi-liquid foods, gel foods, beverages, etc., and are commonly used in the form of capsules, granules, tablets, drinks, etc. Any substrate can be used to prepare soft drinks, juices, coffee, tea, liqueurs, milk, whey beverages, lactic acid bacteria beverages, candies, chewing gum, chocolate, gummi, yogurt, ice cream, puddings, water yolks and the like.

As described above, the solvent extract of soybean embryos and / or soybean hulls of the present invention has the effect of inhibiting the progression of cancer by exhibiting the effect of inhibiting the proliferation of various cancer cells.

That is, it can be seen from FIGS. 1 to 8 that the solvent extract of the soybean embryo and / or soybean hull of the present invention inhibits the proliferation of various cancer cells by inhibiting the proliferation of liver cancer, stomach cancer, colon cancer, and solid cancer cells. .

1 through 8, the growth of various cancer cells is inhibited as the concentration of soybean embryos and / or soybean husk extracts that enhance cancer cell proliferation inhibitory effect is gradually increased, so that the proliferation inhibition rate of cancer cells is treated. It can be seen that it depends on the concentration of the embryo and soy bark extract.

Hereinafter, preferred examples are provided to help understanding of the present invention, but the following examples are merely to illustrate the present invention, and the scope of the present invention is not limited to the following examples.

Example 1 Preparation of Soybean Extract Using Ethanol

About 20 times of 50% ethanol (Duksan, Korea) aqueous solution was added to 20 g of each soybean fraction of soybeans, embryos, and halved soybean hulls, and refluxed for 6 hours using a stirrer at room temperature. The extract thus obtained was filtered with filter paper (whatmann 41), followed by evaporation of the solvent at 40 ° C. for 2 hours, followed by freeze drying to obtain a solvent extract of soybean, embryo, halves, and soybean hulls (hereinafter, “soy, Embryo extract, halves, and shell extract ".

Example 2 Preparation of Soybean Extract Using Hot Water

20 g of soybean, embryo and halves of soybean hulls were added about 10-fold purified water at 80 ° C., followed by stirring extraction at 80 ° C. for 2 hours. The extract thus obtained was filtered with filter paper (whatmann 41) to remove the residue, and then freeze-dried to obtain solvent extracts of soybean, embryo, halves and soybean hulls, respectively.

Example 3 Inhibition of Proliferation of Liver Cancer Cells

The effect of inhibiting the proliferation of liver cancer cells was measured using the solvent extracts of soybeans, embryos, halves, and husks obtained in the above examples.

SK-HEP-1 was used as a liver cancer cell line. Culture of the cancer cell line was added to DMEM (Dulbeco's Modified Egles Medium) with 10% FBS, penicillin (penicillin) 100 IU / ㎖, streptomycin (100 ㎍ / ㎖) using a medium added 5% CO ₂ , 37 ° C. incubator (Forma Scientific Co., Marjetta, OH, USA). The medium compositions used for cell culture were all used by GIBCO BRL (Grand Island, NY, USA).

The cultured SK-HEP-1 cancer cell line was divided into 1 × 10 ⁴ cells in a 96 well plate, and the solvent extract extracted in the above example was prepared at a concentration of 1, 10, 25, 50, 100 mg / ml. And incubated for 72 hours.

In order to measure the degree of inhibition of proliferation of cancer cells, an MTT assay was performed according to a known method (Mosmann, J. Immunol. Method , 65: 55-63, 1983). Cultured cells were washed twice with phosphate buffer. 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl tetrazolium in 96 well plates bromide; MTT, Sigma) was added and reacted at 37 ° C. for 4 hours. The supernatant was removed by centrifuging the 96 well plate at 800 g for 25 minutes at room temperature. 0.1 ml of dimethylsulfoxide was added to the blue formazan product and reacted at 80 rpm for 30 minutes on a shaker, followed by a microplate reader (BioRad, Hercules, CA, USA). Absorbance at 570 nm was measured using. Three repeated experiments were performed according to this example, and the degree of inhibition of cell proliferation of the soybean extract was shown in FIG. 1.

From the above results, it was calculated as IC ₅₀ value that inhibits the proliferation of cancer cell lines by 50%. The ethanol extracts of soybean, halved bean, embryo and husk were 36.8, 40.2, 21.1 and 0.9 mg / ml, respectively. In the case of 36.5, 27.3, 8.5 and 7.0 mg / ml, respectively.

By the above results, it can be seen that the soybean embryos and bark extracts are significantly superior in inhibiting liver cancer growth as a result of comparing the four soybean extracts.

Example 4: breast cancer cell proliferation inhibitory effect

In the same manner as in Example 3, four soybean solvent extracts and hot water extracts, respectively, were treated at various concentrations in MCF 7 as breast cancer cells. As shown in Figures 3 and 4, the IC ₅₀ value of the peel of ethanol extract was the highest as 14.1 mg / ml, the IC ₅₀ value of the peel was also the highest as 5.5 mg / ml in hot water extract. From the above results, it can be seen that the soybean hull extract from the extract is significantly superior in inhibiting breast cancer cell proliferation.

Example 5 Gastric Cancer Cell Proliferation Inhibitory Effect

In the same manner as in Example 3, soybean solvent extract and hot water extract, respectively, were treated at various concentrations in SNU-1, which is gastric cancer cells, and the results are shown in FIGS. 5 and 6. As shown in Figures 5 and 6, for the ethanol extracts of soybeans, halves, embryos and husks, the IC ₅₀ values were 34.4, 29.3, 18.5 and 5.5 mg / ml, respectively, and 80.0, 49.0 and 21.6 for hydrothermal extracts, respectively. , 7.3 mg / ml. As the concentration of the soybean extract was gradually increased by the above results, it was observed that the proliferation of gastric cancer cell lines was inhibited, and that the soybean embryo and bark extract had a remarkably excellent effect of inhibiting gastric cancer cell proliferation.

Example 6 Solid Cancer Cell Proliferation Inhibitory Effect

In the same manner as in Example 3, soybean solvent extract and hot water extract, respectively, were treated at various concentrations in solid cancer cells HT1080, respectively, as shown in FIGS. 7 and 8. As shown in FIGS. 7 and 8, the IC ₅₀ values were 23.7, 26.2, 13.0, and 14.1 mg / ml for the ethanol extracts of soybeans, halves, embryos, and husks, respectively, and 36.8, 40.2, and 21.1 for hydrothermal extracts, respectively. , 0.9 mg / ml. As a result, it can be seen that soybean embryos and bark extracts are remarkably excellent in inhibiting solid cancer cell proliferation.

Therefore, it can be seen that the embryo and bark extracts among the four soybean extracts are remarkably excellent in inhibiting the proliferation of liver cancer, breast cancer, gastric cancer, and solid cancer cells.

As discussed above, the soybean embryo and / or soybean peel extract of the present invention has a remarkably excellent proliferation inhibitory effect against liver cancer, breast cancer, gastric cancer, and solid cancer cell lines, and the extract can be used as a food grade, eliminating toxicity. It can be obtained by inhibiting cancer cell proliferation by ingesting safely without a separate purification process. In addition, according to the present invention by separating and formulating a material that exhibits carcinogenic efficacy by a simple separation process, it is possible to lower the production cost compared to the existing carcinogenic inhibitors, in particular by recycling a large amount of soybean shell discarded during processing There is an advantage to improve the economics.

Claims (10)

delete delete delete Cancer inhibiting composition having a solvent extract of a substance selected from the group consisting of soybean embryos, soybean hulls, and mixtures thereof having cancer cell proliferation inhibitory effect as an active ingredient. The composition for inhibiting cancer according to claim 4, wherein the cancer is applied to liver cancer, breast cancer, gastric cancer, or solid cancer. The method of claim 4, wherein the solvent is water; Or 20-80% ethanol, acetone, or methanol aqueous solution. The anticancer composition according to any one of claims 4 to 6, wherein the cancer inhibiting composition is a pharmaceutical composition and further comprises a pharmacologically acceptable carrier. The composition for inhibiting cancer according to any one of claims 4 to 6, wherein the composition for inhibiting cancer is a processed food or a functional food. The composition for inhibiting cancer according to any one of claims 4 to 6, wherein the composition for inhibiting cancer is a food additive. The composition for inhibiting cancer according to any one of claims 4 to 6, wherein the composition for inhibiting cancer is a functional beverage or a beverage additive.

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