KR101483592B1 - Decreasing Effect in Blood Ethanol Concentration and Improvement of Hepatic Function Including Preparation of Sprouts Hordeum Vulgare Extracts - Google Patents

Abstract

The present invention relates to a composition for improving hangover and hepatic function, comprising the extract of sprout barley as an active ingredient, wherein the composition for hangover sputum containing the sprout barley extract and the fraction according to the present invention is characterized in that it has no side effects on the human body, Since the protective effect is excellent, a composition containing it as an active ingredient can be usefully used as a health functional food, a health food, a drink and an additive for hangover resolution and soy sauce protection.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a composition for reducing blood alcohol concentration or improving hepatic function,

The present invention relates to a composition for reducing blood alcohol concentration or improving liver function comprising an extract of sprout barley as an active ingredient and more particularly to a composition for reducing blood alcohol concentration or liver And a composition for improving function.

Alcohol has long been manufactured and consumed in human society and has been manufactured and sold in almost all countries except for some religious countries. In addition, modern people are increasingly concerned about drinking, and the social and economic damage caused by alcohol is increasing, which is a serious concern for the public health. Excessive intake of alcohol is associated with various side effects such as thirst, fatigue, amnesia, dyspepsia, vomiting, and vitamin deficiency. The risk of alcoholism increases with the amount and frequency of alcohol consumption.

In particular, alcohol consumption in Korea is the highest in the world. Excessive consumption of alcohol affects almost all organs of the body and is reported to cause many diseases such as liver disease, gastritis, pancreatitis, and metabolic syndrome.

Normal alcohol metabolism is absorbed in the stomach or small intestine when alcohol is ingested, 10% of which is excreted by breathing, sweat and urine, and the remaining 90% of alcohol enters the blood vessels and is metabolized in the liver. Alcohol dehydrogenase (ADH), which is present in hepatocytes, oxidizes alcohol to acetaldehyde, which is a toxic substance, and acetaldehyde is again reduced in hepatocytes by acetaldehyde dehydrogenase (ACE) dehydrogenase, ADLH), and finally it is transferred to muscle or fat tissue and finally decomposed into carbon dioxide and water. However, in the case of excessive alcohol consumption due to excessive alcohol consumption, abnormal metabolism of alcohol is caused by non-metabolized ethyl alcohol and acetaldehyde, resulting in hangover of head, heavy, sick, . Therefore, it is necessary to develop new biomedical materials, functional food materials, and well - being materials to meet the social phenomenon changing at a very fast pace and increasingly diverse consumers' expectations.

This development is based on the contents of the hangover relieving which is the active ingredient of the first extract of sprouts barley which is studied both at home and abroad. The results of research on some hangovers are as follows: Drinking hangovers and drinking drinks are classified into three types, namely, condition using Lotus seed, lot seed, rice embryo (CJ company), high purity chitosan (Chong Kun Dang) And 808 (Grammy), which is made by using extracts. The present invention also relates to a composition for hangover decomposing which contains an extract of green tea and green tea (Japanese Patent Application Laid-Open No. 10-2005-0100890), a hangover resolution composition containing a green leaf extract of Hovenia dulcis (patent 10-2011-0056014) Patent No. 10-2010-0108112), " a method for detoxifying hangover and a method for inhibiting alcoholic gastric ulcer in a purple sweet potato fermented filtrate fraction, etc. " Although it has been reported that sanitizing agents originating from various plants, such as mountain ginseng, milk seedlings, and duckwood extracts, have a function of hangover resolution, only some of the various factors of drinking are effective or have little effect, Is intended to simply utilize the efficacy of a natural tonic or a remedy.

Therefore, it is urgent to develop an extract and an effective ingredient for a new hangover solution and soy sauce protection which are safe for human body and have no side effects and sufficient effect of hangover resolution.

      Sprout barley (young barley leaves) is a young leaf that is harvested about 8-15 days after sowing barley seeds. It has high content of inorganic components such as calcium, magnesium and potassium as well as excellent content of vitamin B1 and vitamin C (Patent Application: 10-2004-0045798, method of producing barley leaf tea, Kim Dong Won et al.), And also saponin, lutonarin and antioxidant which have antioxidant, anti-inflammatory and anti- (Benedet JA et al., J. Agric. Food. Chem., 2007, 55, 5499-5504). In addition, metabolic syndrome such as hypertension, diabetes, and lipid metabolism abnormality (Patent Application: 10-2011-0116385), which is effective for health-functional foods and pharmaceuticals.

    In the present study, the extract of sprout barley showed the effect of lowering the blood alcohol concentration in a single dose test using rodent. The effect of reducing blood alcohol concentration was evaluated as a result of marked reduction of blood cholesterol and triglyceride concentration in blood biochemical markers and increased in vitro cell viability using Raw267.7 and Huh7 cells, This increase in hepatic cell activity is believed to promote ADH and ALDH functions essential for alcohol metabolism (2).

Accordingly, the present invention relates to the field of utilization of health foods, pharmaceutical compositions and beverages, which are effective for eliminating hangover and liver function due to alcohol, and effective ingredients of the extracts of barley extract, which have not been studied in the past, And the use thereof.

Therefore, the inventors of the present invention found that the extract of bud of barley did not show cytotoxicity during the search for the functional material derived from the bud of barley, and showed the effect of decomposing alcohol by promoting the action of ADH and ALDH through the increase of hepatic cell activity, Evaluation of blood biochemical test results also showed that cholesterol and triglyceride decreased. In addition, the results of experiments on the increase of cell viability in vitro using Raw267.7 and Huh7 cells showed that the increase of hepatic cell activity promotes the action of ADH and ALDH essential for alcohol metabolism.

Through the present invention, it was firstly found that the extract of sprout barley and its active ingredient are involved in the improvement of hepatocyte activity (3) alcohol decomposition (4) hangover resolution and liver function improvement, and hangover resolution and liver function Health foods, beverages, natural products, pharmaceuticals, additives, and the like.

Accordingly, it is an object of the present invention to provide a pharmaceutical composition for improving hangover and liver function comprising a bud or extract of barley or an active ingredient.

Another object of the present invention is to provide a method for preparing a pharmaceutical composition for hangover relief and hepatic function improvement, which comprises a germinated barley extract or a germinated barley solvent fraction.

Other objects and advantages of the present invention will become more apparent from the following detailed description of the invention, claims and drawings.

According to one aspect of the present invention, there is provided a pharmaceutical composition for improving hangover resolution and liver function comprising an extract of sprouts barley as an active ingredient.

According to another aspect of the present invention,

i) extracting the dried barley with water, a lower alcohol having 1 to 4 carbon atoms, a fermented alcohol or a mixed solvent thereof by sowing, cultivating, harvesting, drying and pulverizing the barley seeds; And a method for preparing a pharmaceutical composition for improving hangover and improving liver function.

According to still another aspect of the present invention, there is provided a health functional food, a health food, a drink, a natural medicine, and an additive for relieving a hangover and improving liver function, which comprises an extract of sprouts barley as an active ingredient.

The features and advantages of the present invention are summarized as follows.

(i) The pharmaceutical composition comprising the bud of the present invention exhibits an effect of improving hangover and improving liver function by increasing hepatic cell activity.

(ii) The sprout barley extract of the present invention is a safe natural material derived from sprouts barley, and thus is useful as a pharmaceutical composition for relieving hangover and liver function safely in the human body, a health functional food, a health food, a drink, a natural medicine and an additive .

FIG. 1 is a graph showing the increase in bowing performance at all treatment concentrations except for the highest concentration of macrophage (Raw 264.6) according to the concentration and time of treatment with barley extracts.
FIG. 2 is a graph showing the increase in hepatic cancer cell activity at all treatment concentrations except for the highest concentration of hepatocellular carcinoma cell line (Huh-7) according to the concentration and time of treatment with barley extracts.
FIG. 3 is a graph showing the treatment concentration of the barley extract and the active ingredient in the single-alcohol test using rodents.
FIG. 4 is a graph showing the effect of improving the residual alcohol content of blood according to the concentration of bud bud administration after administration according to FIG. 3; FIG.
FIG. 5 is a graph showing the increase of mRNA expression of ADH and ALDH, two enzymes involved in alcohol degradation in liver cells at different concentrations when administered with bud.
FIG. 6 is a histopathological observation for evaluating the protective effect of hepatocyte on the administration concentration of the extract obtained from the administration of the extract of bud.
FIG. 7 is a graph showing the effect of hepatocyte growth inhibition on hyperchromatic hepatocyte, binucleated hepatocyte, swelling of hepatocyte and activation of Kupffer cell, (0, 1, 2, and 3). The results of this study are as follows.
FIG. 8 is a histopathological observation of the kidney cortex area for evaluating the effect on the kidney of each concentration when the bud of barley extract is administered.
FIG. 9 is a histopathological observation of the renal water quality area for evaluating the effect on the kidney of each concentration when administered with bud of the bud.
FIG. 10 shows the effects of the extracts of buds on the growth of kidney by Bowman's capsule hyperplasia and / or degeneration, mesangial cell hyperplasia, (0, 1, 2, and 3) by histopathological observations on the degree of cell proliferation, Juxtaglomerular cell hyperplasia, renal tubule degeneration, and hyaline droplet / cast This is a graph that shows the result.

According to one aspect of the present invention, there is provided a pharmaceutical composition for improving hangover resolution and liver function comprising an extract of sprouts barley as an active ingredient.

According to a preferred embodiment of the present invention, the pharmaceutical composition comprising the germanium barley extract as an active ingredient is effective in removing hangover and improving liver function. The present invention relates to a sprout barley extract as a pharmaceutical composition for hangover resolution and hepatic function improvement, but is not limited thereto. The sprout barley extract may be treated with a suitable solvent to obtain a bud broth solvent fraction.

In the present specification, the term " germanium barley extract " means barley seeds obtained by sowing, harvesting, drying and pulverizing after 8-15 days of cultivation. (B) an anhydrous or hydro-lower alcohol having 1-4 carbons such as methanol, ethanol, propanol, butanol, n-propanol, iso Propanol and n-butanol), (c) a mixed solvent of the lower alcohol and water, (d) acetone, (e) ethyl acetate, (f) chloroform, (g) 1,3-butylene glycol h) hexane, (i) diethyl ether, or (j) butyl acetate.

In the present specification, the term " bud of barley solvent fraction " means an active fraction obtained by fractionation of the bud of barley extract with activity for the purpose of the present invention using the solvent used for the extraction. The solvent used for obtaining the bud leaf solvent fraction may be any extraction solvent conventionally used in the art, and preferably (a) an anhydrous or a lower alcohol having 1 to 4 carbon atoms (e.g., methanol, ethanol, propanol (B) a mixed solvent of the lower alcohol and water, (c) acetone, (d) ethyl acetate, (e) chloroform, (f) 1 , 3-butylene glycol, (g) hexane, (h) diethyl ether, or (i) butyl acetate.

As used herein, the term " extract " has the meaning of being used as a crude extract in the art as described above, but broadly includes the above-mentioned solvent fraction.

The barley extract or fraction of the present invention can be prepared in powder form by an additional process such as vacuum distillation and freeze drying or spray drying.

When the composition of the present invention is manufactured from a pharmaceutical composition, the pharmaceutical composition of the present invention includes a pharmaceutically acceptable carrier. The pharmaceutically acceptable carriers to be contained in the pharmaceutical composition of the present invention are those conventionally used in the present invention and include lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia rubber, calcium phosphate, alginate, gelatin, But are not limited to, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrups, methylcellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. It is not. The pharmaceutical composition of the present invention may further contain a lubricant, a wetting agent, a sweetening agent, a flavoring agent, an emulsifying agent, a suspending agent, a preservative, etc. in addition to the above components. Suitable pharmaceutically acceptable carriers and formulations are described in detail in Remington ' s Pharmaceutical Sciences (19th ed., 1995).

The pharmaceutical composition of the present invention can be administered orally or parenterally, and is preferably applied by parenteral administration, more preferably topical application by application.

The appropriate dosage of the pharmaceutical composition of the present invention may vary depending on factors such as the formulation method, administration method, age, body weight, sex, pathological condition, food, administration time, administration route, excretion rate, . The dosage of the germinated barley extract and fraction, which is a physiologically active substance contained in the pharmaceutical composition of the present invention, is 0.001-100 mg / kg, preferably 0.1-100 mg / kg, more preferably 5-50 mg / kg. < / RTI >

The pharmaceutical composition of the present invention may be formulated into a unit dose form by formulating it using a pharmaceutically acceptable carrier and / or excipient according to a method which can be easily carried out by a person having ordinary skill in the art to which the present invention belongs. Or by intrusion into a multi-dose container. The formulations may be in the form of solutions, suspensions, syrups or emulsions in oils or aqueous media, or in the form of excipients, powders, powders, granules, tablets or capsules, and may additionally contain dispersing or stabilizing agents.

According to a preferred embodiment of the present invention, the shoot barley solvent fraction obtained from the shoot barley extract can be extracted with water, a lower alcohol having 1-4 carbon atoms, a fermented alcohol or a mixed solvent thereof.

According to a preferred embodiment of the present invention, the germanium barley extract or the germanium barley solvent fraction promotes the action of liver cells, thereby promoting the production of a decomposition enzyme required for alcohol metabolism, thereby lowering the blood alcohol concentration and exhibiting a hangover function, (Cholesterol) and triglyceride (Triglyceride) suppresses the activity of improving liver. (5)

According to another aspect of the present invention, there is provided a process for producing a microorganism which comprises seeding, cultivating, harvesting, drying and pulverizing a bud of barley, pulverizing the resulting barley with water, a lower alcohol having 1-4 carbon atoms, a fermented alcohol, ; , The fractions containing the compounds of the present invention exhibit a function of decreasing the alcohol concentration of the blood by decreasing the alcohol concentration and promoting the production of alcohololytic enzymes by increasing the hepatic cell activity and exhibiting the hangover function and inhibiting cholesterol and triglyceride, And a manufacturing method thereof.

According to still another aspect of the present invention, there is provided a health food for improving hangover and improving liver function, comprising as an active ingredient a bud or barley extract fraction of bud or barley.

The composition of the present invention can be added to health functional foods for the purpose of hangover resolution and liver function improvement. When the fragrant barley solvent fraction of the present invention is used as a food additive, the fragrant barley fraction may be used as it is or may be used in combination with other food or food ingredients, and may be appropriately used according to a conventional method.

According to a preferred embodiment of the present invention, the health food can be manufactured in a form selected from the group consisting of powder, granule, tablet, capsule and beverage.

When the composition of the present invention is prepared as a food composition, it contains not only the barley extract or the fraction as an active ingredient but also a component which is ordinarily added at the time of food production. Examples thereof include protein, carbohydrate, fat, And flavoring agents. Examples of the above-mentioned carbohydrates are monosaccharides such as glucose, fructose, and the like; Disaccharides such as maltose, sucrose, oligosaccharides and the like; And polysaccharides such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. Natural flavorings such as tau martin and stevia extract (e.g., rebaudioside A and glycyrrhizin) and synthetic flavorings (saccharine, aspartame, etc.) can be used as flavorings.

 For example, when the food composition of the present invention is prepared as a drink, citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, juice, mulberry extract, jujube extract, licorice extract, Can be included.

The amount of the active ingredient to be mixed can be suitably determined according to the intended use (prevention, health or therapeutic treatment). Generally, in the production of foods or beverages, the shoot barley solvent fraction of the present invention is added in an amount of not more than 15% by weight, preferably not more than 10% by weight based on the raw material. However, in the case of long-term consumption intended for health and hygiene purposes or for health control purposes, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount exceeding the above range .

There is no particular limitation on the kind of the food. Examples of the food to which the above substances can be added include dairy products including meat, sausage, bread, chocolate, candy, snack, confectionery, pizza, ramen, other noodles, gums, ice cream, various soups, drinks, tea, Alcoholic beverages, and vitamin complexes, all of which include health functional foods in a conventional sense.

In addition to the above, the composition of the present invention may further contain various nutrients, vitamins, electrolytes, flavors, colorants, pectic acids and salts thereof, alginic acid and its salts, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, A carbonating agent used in a carbonated beverage, and the like. In addition, the composition of the present invention may contain flesh for the production of natural fruit juices, fruit juice drinks and vegetable drinks. These components may be used independently or in combination. Although the ratio of such additives is not critical, it is generally selected in the range of 0.01 to 0.20 parts by weight per 100 parts by weight of the composition of the present invention.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention, and it is to be understood by those skilled in the art that the present invention is not limited thereto It will be obvious.

One. Example  1: Preparation of barley extract

Rural Development Administration After the seed barley was sown in the functional crop area of the National Institute of Food Science and Technology, the barley was harvested after 8-15 days of harvesting, dried and pulverized. 5kg of the barley powder was extracted with 40L of fermented alcohol as an extraction solvent and stirred at room temperature for 24 hours. After filtering through a No. 2 paper filter, the filtrate was concentrated using a reduced pressure concentrator to obtain a deep dark green mushroom barley extract, which was used as a sample in the following experimental example.

2. Experimental Example  1: cell Bow performance  exam

(1) cells of macrophage cell line Bow performance  exam

After treatment of macrophage cell line (Raw 264.7) and hepatoma cell line (Huh-7) with 15.625, 62.5, 125, 250, 500 and 1000 μg / 5-dimethylthiazol-2-yl) -2,5-diphenyl-tetrazolim bromide). The macrophage (FIG. 1) and liver cancer cells (FIG. 2) were counted in 96 wells at 2 × 10 ² cells / well and cultured in the above concentrations. After 24 and 72 hours, the MTT solution In addition, formazan was dissolved in dimethyl sulfoxide solution, and the degree of dissolution of formazan was measured at optical density (OD value) at 595 nm as relative cell activity (% ).

In FIG. 1, the macrophage cell line Raw264.7 was treated with 15.625, 62.5, 125, 250, 500 and 1000 ㎍ / ㎖ of the extracts of shoot barley and the absorbance was measured at OD595 nm at 24 hours and 72 hours The cell viability was observed in (A) 24 hrs, but the cellulase activity was increased depending on the concentration of the extract of barley extract, compared with the control group in which no treatment was performed except for the treatment group of 1000 ㎍ / ㎖ Respectively. (Cell proliferation) of Raw264.7 cells was observed at 72 hours (B) except for the highest concentration of bud - extract extract.

(2) Bow performance  evaluation

As shown in FIG. 2, the cell active activity of the extract of the barley extract against the hepatoma cell line, Huh7, was evaluated to be dependent on the concentration of the extract of barley extract at 24 hours after the test substance treatment (C) (D) The results of 72 hours showed that the cellulosic performance was generally higher than that of the control group, although the increase was not dependent on the extract of barley extract.

3. Experimental Example  2: in vivo  How to treat sprout barley extract

To evaluate the effect of barley extract on alcohol degradation, SD rats were administered with 40 ml of ethanol, which lost the upright reflex, in SD rats at a dose of 10 ml / kg (body weight). As shown in FIG. 3, after the administration of alcohols, the extracts of barley barley were added at a concentration of 10 mg / kg (T1), 1500 mg / kg (T2), 500 mg / kg (T3) and 250 mg / / kg < / RTI >

4. Experimental Example  3: Improvement of Blood Alcohol Concentration on Extracts of Barley Extract

In the same manner as in Experimental Example 2, alcohols and barley extracts were administered to SD rats and blood was collected from the venous blood at 10, 60, 180, 360 minutes and 24 hours. Serum was separated from the blood and dichromate The residual alcohol concentration in blood was measured by colorimetric determination using absorbance at 580 nm. The results are shown in FIG.

Blood alcohol concentration was highest at 60 min in both control and sprout barley extracts at doses of 3000 mg / kg (T1), 1500 mg / kg (T2), 500 mg / kg (T3), and 250 mg / kg , And then gradually decreased. Blood alcohol was decreased in all test groups at 60 min of alcohol administration compared to the control group, except for the T4 group, which received the lowest dose of 250 mg / kg of bud.

In the measurement time after 60 min, the alcohol concentration was decreased in the barley extract group. It is considered that 500 mg / kg and 1500 mg / kg are the most effective in decreasing blood alcohol concentration in the doses of 3000 mg / kg, 1500 mg / kg, 500 mg / kg and 250 mg / kg of bud.

5. Experimental Example  4: Effect of Extracts of Barley Extract on Increase of Alcohol Degradation

After 24 hours, blood samples were taken to observe changes in blood alcohol concentration. Animals were sacrificed and liver tissues were collected from each animal. For histopathological analysis, liver tissue was treated with 10% formalin And partially frozen at 70 ° C for RT-PCR for analysis of changes in the expression levels of ADH and ALDH in liver cells. The results of the RT-PCR test using this frozen liver sample are shown in FIG. 5 below.

The increase in mRNA expression of ADH and ALDH, two enzymes involved in alcohol degradation in liver cells after administration of shoot barley extract, was observed in the liver of liver treated with 500 mg / Enzyme was highest observed. ADH was increased by about 20% and ALDH by 38% compared with the control group. These results indicate that the mRNA increase of the two enzymes influenced lowering of blood alcohol concentration as shown in Fig.

6. Experimental Example  4: Effect of barley extract on liver function improvement

In the same manner as in Experimental Example 2, alcohol and sprout barley extract were administered to SD rats and blood was collected from the venous blood at 10, 60, 180, 360 minutes and 24 hours, Serum concentrations of asparate transaminase, alanine transaminase (ALT), cholesterol and triglyceride were measured and the results were as follows.

In serum chemistry test results for AST and ALT, the AST increased over time in both the control group and the barley extract group after 360 minutes of alcohol administration. At 24h, the control group showed a decrease in AST However, it was observed that the group treated with barley extract had a high value continuously.

In the case of Cholesterol, it was observed that the control group showed a slightly higher tendency than the barley extract group in the control group and the barley extract group.

In the early stage of blood alcohol concentration (10 min ~ 60 min), the concentration of TG was lower in the group treated with the barley extract than in the control group, and then the concentration of TG was also decreased The treatment group and the control group showed similar levels.

7. Experimental Example  5: Histopathological Experiment with Extract of Sprout Barley - Liver

In the same manner as in Experimental Example 2, the alcohol and the barley extract were administered to SD rats, and the animals were sacrificed at 24 hours, the abdominal cavity was opened, the liver tissues were taken out from each animal, fixed in 10% neutral formalin, After the tissue processing, slides were made through the paraffin embedded. For histopathological observation, each slide was stained with hematoxylin-eosin and observed under an optical microscope (Fig. 6).

As a result of the experiment, the maximum dose of 3000 mg / kg of barley extract did not show liver toxicity in vivo. The inhibitory effects of alcohol extracts on hepatocyte degeneration (hyperchromatic hepatocyte, binuclear hepatocyte, activation of kupffer cell and swelling of hepatocyte) were observed at 1500 mg / kg (T2) and 500 mg / kg (T1)

In order to evaluate the effects of alcohol and shoot barley extract on liver tissues, the effects of hyperchromatic hepatocyte, binucleated hepatocyte, swelling of hepatocyte and Cooper cell activity Activation of Kupffer cell) was graded from 0 to 4, and the scores were expressed as differences between groups (FIG. 7).

As a result of the evaluation of the histopathological findings, the results of the 4th step (0, 1, 2 and 3) showed that the control group had a liver protection value of 1.1, 500 mg / kg (T3) in the administration group showed the lowest value of 0.5 and was found to be most effective for liver protection (Fig. 7).

 8 . Experimental Example  6: Histopathological Experiment with Extract of Barley Extract - Kidney

In the same manner as in Experimental Example 2, alcohols and buds of barley extract were administered to SD rats. After 24 hours, the animals were sacrificed and the abdominal cavity was opened. The kidney tissues were extracted from each animal, fixed in 10% neutral formalin, After the tissue processing, slides were made through the paraffin embedded. For histopathological observation, hematoxylin-eosin staining was performed and observed under an optical microscope (Figs. 8 and 9). To evaluate the effect of alcohol and shoot barley extract on kidney tissue, Bowman's capsule hyperplasia and / or degeneration, mesangial cell hyperplasia, glomerular cell proliferation (proliferation of radionuclide, Juxtaglomerular cell hyperplasia, renal tubule degeneration and vitreous material (hyaline droplet / cast) were graded from 0 to 4 and scored as differences between groups (FIG. 10).

Significant renal tubular degeneration was observed in both the control and bud - barley extract - treated groups, as well as the formation of vitreous material in the tubules. Especially, T1 (3000 mg / kg) and T2 (1500 mg / kg) groups showed higher tendency of renal tubular degeneration and vitreous substance formation compared to the control group. T3 (500 mg / kg) and T4 250mg / kg) group showed lower tendency than the control group. The degree of renal tubular degeneration and the formation of vitreous material showed a similar tendency to the overall evaluation of renal tissue (Fig. 10). However, in the evaluation of Bowman's cell proliferation and denaturation, proliferation of vascular mesangial cells, and proliferation of glomeruli, proliferation of two groups, ie, T1 and T2, in the high-dose group of shoot barley extract, Lt; / RTI >

Claims (8)

A pharmaceutical composition for reducing blood alcohol concentration and improving liver function, comprising as an active ingredient a bud of barberry extract obtained by extracting bud of barley with fermentation alcohol.
[3] The pharmaceutical composition according to claim 1, wherein the germanium barley extract comprises up-regulation of an alcohol dehydrogenase (ADH) or an acetaldehyde dehydrogenase (ALDH) by hepatocyte activity. .
delete [Claim 2] The pharmaceutical composition according to claim 1, wherein the extract has a concentration of 100 to 1500 mg / 10 ml / kg.
(i) seeding, cultivating, harvesting, drying and pulverizing the bud barley; And
(ii) extracting the powdered germanium barley using a fermentation alcohol;
A method for producing a pharmaceutical composition for reducing blood alcohol concentration and improving liver function.
A food composition for eliminating hangover and improving liver function, comprising the extract of Seabrokia barley according to any one of claims 1, 2, and 4 as an active ingredient.
A health food comprising the food composition for the hangover resolution and liver function improvement of claim 6.
The health food according to claim 7, wherein the health food is a form selected from the group consisting of powder, granule, tablet, capsule and beverage.

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