KR101935861B1 - Functional food composition for removing hangover and improving liver function and manufacturing method for thereof - Google Patents

Abstract

The present invention relates to a health functional food composition for removing hangover and improving liver functions, containing a complex extract of radish, wood-cultivated ginseng, ginger, garlic, pear, bellflower root, Sinapis alba, Zizyphus jujuba Miller, Amomum villosum Loureiro or Amomum villosum and Ziziphus zizyphus, and to a manufacturing method thereof. Accordingly, provided are the health functional food composition for removing hangover and improving liver functions, which has excellent effects of removing hangover caused by alcohol and protecting liver from alcoholic liver damage and has improved effect of improving liver functions, and the manufacturing method thereof.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention [0001] The present invention relates to a health functional food composition for improving hangover and improving liver function, and a method for producing the same. 2. Description of the Related Art Health Functional Food Composition <

The present invention relates to a health functional food composition for hangover resolution and liver function improvement and a method for producing the same.

The liver is the next big organs in our body, and it is a very important organ with many functions such as energy metabolism of lipids, detoxification of toxic substances such as various drugs and alcohol, excretion of bile, hematopoiesis, blood coagulation and circulating blood volume control .

The blood ethanol absorbed in the body is converted to acetaldehyde by alcohol dehydrogenase (ADH) in hepatocytes and acetaldehyde is converted into acetate by acetaldehyde dehydrogenase (ALDH) And finally decompose into carbon dioxide and water.

GOT (Glutamic oxalacetic transaminase, Aspartate aminotransferase, Aspartate aminotransferase), GPT (Glutamic pyruvate transaminase, ALT, alanine aminotransferase, and alanine aminotransferase), which are used as main indicators of liver function, Enzymes involved in metabolism are damaged by hepatocyte damage and are released into the blood, resulting in an increase in blood levels.

The hangover phenomenon is caused by vomiting, diarrhea, dizziness, thirst, general boredom, fatigue, memory loss, abdominal bloating, dyspepsia, vitamin deficiency, etc. due to ethanol and acetaldehyde accumulated in the body without being decomposed by carbon dioxide and water Is a phenomenon that occurs.

Acetaldehyde dehydrogenase has type Ⅱ, which acts from low concentrations of acetaldehyde, and type Ⅰ, which requires high concentration of acetaldehyde. Compared to other species, Asians have a deficiency or lack of type II acetaldehyde enzyme, so the decomposition of acetaldehyde is slow. Therefore, acetaldehyde accumulated in the body without decomposition often causes various hangover phenomena to be felt more often.

Alcoholic liver diseases include fatty liver caused by the accumulation of triglyceride in liver, alcoholic hepatitis causing inflammatory damage caused by alcohol, liver cirrhosis in which hepatic tissue is transformed into fibrotic tissue due to chronic inflammation liver cirrhosis).

Therefore, there have been various studies on the products to solve hangover due to excessive drinking, and as a result, various health supplement foods are being developed.

To date, there are milk cedar extract, broccoli sprout powder, shiitake mushroom mycelium, mushroom mycelium extract, and bokbunja extract powder from Korea Food and Drug Administration 's "help for liver health" Helping to protect "as the raw material to recognize the efficacy of Hovenia japonica extract, lactic acid bacteria fermented seaweed extract, and so on. In the last decade, foods of various form using the above-mentioned raw materials have been developed, the majority of which are limited to drinks. In addition, these products are believed to have only a slight physiologic improvement effect or a minimal clinical effect in scientifically proving the liver function improvement and the hangover resolution effect.

Accordingly, there is a growing need for the development of a food composition that improves hangover symptoms caused by drinking and improves functionality that helps protect the liver from alcoholic damage.

One object of the present invention is to provide a health functional food composition for improving hangover and improving liver function, which is excellent in the effect of relieving hangover due to drinking and protecting the liver from alcoholic liver damage and improving liver function, and a method for producing the same .

One embodiment of the present invention relates to a health functional food composition for improving hangover and liver function, which comprises a complex extract of radish, goat ginseng, ginger, garlic, pear, platycodon, white raspberry, will be.

Wherein the combined extract comprises 30 wt.% To 70 wt.% Of no extract, 1 wt.% To 20 wt.% Ginseng, 5 wt.% To 35 wt.% Ginger, 5 wt.% To 25 wt.% Garlic, 5 wt. Wherein the function of the mixture comprising 5% to 30% by weight, 0.5% to 5% by weight of white rice, 0.5% to 5% by weight of acidophore, 0.5% to 5% Ethanol extract.

The health functional food composition may further include one or more additives selected from honey, persimmon and oligosaccharides.

The health functional food may be a powder, granule, tablet, capsule, ring, liquid tincture or a processed product in the form of a drink.

In another embodiment of the present invention, the combined extract comprises 30 wt.% To 70 wt.%, 1 wt.% To 20 wt.% Ginseng, 5 wt.% To 35 wt.% Ginger, 5 wt. 0.5 to 5% by weight of cornstarch, 0.5 to 5% by weight of cornstarch, 0.5 to 5% by weight of jujube, and 5 to 30% By weight of water and ethanol at a volume ratio of 1: 0.1 to 1: 10, and then extracting the mixture at 20 to 100 DEG C for 1 to 72 hours to prepare a combined extract. And a method for producing a health functional food composition for improving liver function.

The present invention provides a health functional food composition for improving hangover and hepatic function, which has an effect of eliminating a hangover due to drinking and an effect of protecting the liver from alcoholic liver damage and improving liver function, and a method for producing the same have.

Fig. 1 shows the results of the alcoholysis activity measurement of Experimental Example 1 of the present invention.
Fig. 2 shows the results of measurement of activity of acetaldehyde degrading enzyme of Experimental Example 2 of the present invention.
Fig. 3 shows the result of the short hangover efficacy evaluation of Experimental Example 3 of the present invention.
4 shows the results of the hangover elimination sensory evaluation of Experimental Example 4 of the present invention.
FIG. 5 shows the results of an experiment for verifying hepatocyte protective effect of Experimental Example 5 of the present invention.
FIG. 6 shows the results of evaluation of ethanol reduction effect in blood of Experimental Example 6 of the present invention.
Fig. 7 shows the results of evaluation of blood acetaldehyde reduction effect of Experimental Example 7 of the present invention.
FIG. 8 shows the results of measurement of blood glutamate oxalacetic acid transferase activity of Experimental Example 8 of the present invention.
9 shows the results of measurement of pyruvate transglutaminase activity in blood of Experimental Example 9 of the present invention.

One embodiment of the present invention relates to a health functional food composition for improving hangover and liver function, which comprises a complex extract of radish, goat ginseng, ginger, garlic, pear, platycodon, white raspberry, will be.

Accordingly, the present invention provides a health functional food composition for relieving hangover caused by alcohol and an effective effect of protecting the liver from alcoholic liver damage and improving liver function and for improving hangover and liver function, and a method for producing the same .

The combination extract of radish, goat ginseng, ginger, garlic, pear, platycodon, white sprout, acidophilus, konjin and jujube contained in the above-mentioned complex extract has a remarkably excellent hangover resolution and liver function A health functional food composition having an improving effect can be realized.

The radish is not particularly limited as long as it is a root vegetable of crucifers and Raphanus sativus plants. It has a high moisture content, low calorie content, and a lot of fiber. In addition, an inorganic substance such as calcium, potassium, vitamin, etc., an enzyme such as diastase and peroxycytidine for promoting digestion and absorption can be provided, and it can give an effect of preventing stomach pain and gastric ulcer.

The extract of the mulberry has excellent effect of increasing hangover activity (measured by alcohol dehydrogenase activity assay method) when used as an ethanol extract or a functional ethanol extract of alcohol, and it is an alcohol dehydrogenase enzyme necessary for decomposing alcohol The activity can be increased.

The ginseng is the root of ginseng (Panax ginseng C. A. Meyer, Araliaceae and Araliaceae) planted by seeding or seedling transplantation. Species are not particularly limited. The ginseng (or ginseng) contains 0.10% by weight or more of ginsenoside Rg1 and 0.20% by weight or more of ginsenoside Rb1 relative to the dried product. Thus, the ginseng root (or ginseng) , Fatigue recovery, anti-aging, and the like.

The goat ginseng can be used as an ethanol extract or a functional ethanol extract in a complex extract to further improve the hangover resolution activity due to the stimulation of metabolism.

The ginger is not particularly limited as long as it is a root of Zingiberaceae plant ginger (Zingiber officinale Roscoe). Ginger can be supplied with active ingredients such as Farnesol, Cineole, Zingiberene, Zingiberol, Gingerone and Shogaol to give anti-inflammatory effects. .

The ginger can be used as an ethanol extract or a functional ethanol extract in a complex extract to activate the metabolism and diuretic action (directional structure), to prevent the growth of harmful microorganisms in the stomach, and to protect against alcoholic damage by the active ingredients Can be implemented.

The garlic is not particularly limited as long as it is a lily and a root of garlic (Allium sativum) which is a perennial herbaceous plant. Garlic contains a lot of moisture, calcium, phosphorus, iron, potassium, and amino acid, and can supply antioxidant and anti-inflammatory effect by supplying a high content of effective ingredient such as cystine, histidine and lysine.

When garlic is used as an ethanol extract or a functional ethanol extract in a complex extract, scorodinin, selenium, vitamin A, vitamins B1 and B2, and vitamin C can be supplied in a more excellent ratio, and a combination of allicin and vitamin components It is possible to increase the absorption rate of the active ingredient, and to realize a tanning effect and a protection effect against alcoholic damage.

The embryo is not particularly limited as long as it is a fruit of a pear tree, a deciduous tree belonging to the genus Pomoideae, a pear tree (Pyrus), or a shrub plant, in Rosaceae. Boats can relieve thirst and hangover effects, activate diuretic action, and promote hepatic activity, thereby rapidly detoxifying alcohol in the body.

The bellflower (Platycodon grandiflorum (JACQ) A. DC.) Is not particularly limited as long as it is a root of a perennial plant belonging to the genus Chrysanthemum. The bellflower is supplied with active ingredients such as carbohydrate, calcium, iron protein, lipid, ash, inulin, phytosterol, platycodin, polygalacin and saponin, , Analgesic action, and gastric acid secretion inhibitory effect.

When the bellflower is used as an ethanol extract or a functional ethanolic extract in a complex extract, it is possible to achieve the effect of eliminating the hangover by the active ingredients and the protective effect against the alcoholic damage.

The white scallops were dried with the mustard seeds of the Chinese cabbage (Brassica Cernua Forb. Et Hemsl. Дв. Б. B. juncea Coss. White ㎖ B. alba (L.) Boiss. Black mustard B. nigra Koch) It is not particularly limited.

The above-mentioned product of the present invention can be used as an ethanol extract or a functional ethanol extract in a combination extract to activate metabolism and diuretic action, to prevent the growth of harmful microorganisms in the stomach, and to protect against alcoholic damage caused by antioxidant and antiinflammatory active ingredients Can be implemented.

The acidophilus is not particularly limited as long as it is a seed of Rhamnaceae Sanjoz (Zizyphus jujuba Miller var. Spinosa Hu ex H. F. Chou). Sanjoin can serve as an effective ingredient for Betulic acid, Betulin, Fatty oil, Beta-Sistosterol, Protein, and Organic acid.

The acidophilus, when used as an ethanol extract or a functional ethanol extract in a complex extract, imparts a sedative effect (consciousness), a soothing effect, and a blood pressure lowering effect, Hypertension, and the like.

The construction worker is a fruit or seed of Zingiberaceae plant (Amomum villosum Loureiro var. Xanthioides T. L. Wu et Senjen) or Amomum villosum Loureiro, and the species is not particularly limited.

When the extract is used as an ethanol extract or a functional ethanol extract in a combination extract, the agent promotes metabolism and diuretic action, provides a soothing effect, further enhances effects such as blood pressure lowering, promotes decomposition of the body alcohol, Symptoms such as nervous excitement, headache, and hypertension can be relieved.

The jujube is not particularly limited as long as it is the fruit of the Rhamnaceae jujube (Zizyphus jujuba Miller var. Inermis Rehder) or the jujube jujuba (Zizyphus jujuba Miller var. Hoonensis T. B. Lee). The jujube contains the saponin components (Zizyphussaponin I, II, III), Rutin, Scopoletin, vitamin A, C, thiamine, riboflavin, niacin, and the like.

When the jujube is used as an ethanol extract or a functional ethanol extract in a complex extract, the extraction efficiency of the alkaloid component and the polyphenolic compound is further enhanced to activate the metabolism and the diuretic effect, the soothing effect and the blood pressure lowering effect To promote the decomposition of alcohol in the body, and can relieve symptoms such as nervous excitement, headache and hypertension due to hangover.

Wherein the combined extract comprises 30 wt.% To 70 wt.% Of no extract, 1 wt.% To 20 wt.% Ginseng, 5 wt.% To 35 wt.% Ginger, 5 wt.% To 25 wt.% Garlic, 5 wt. Wherein the function of the mixture comprising 5% to 30% by weight, 0.5% to 5% by weight of white rice, 0.5% to 5% by weight of acidophore, 0.5% to 5% Ethanol extract. Within this content range, it is possible to provide a health functional food composition for hangover relief and liver function improvement, which is excellent in the effect of eliminating hangover due to drinking, protecting liver from alcoholic liver damage, and improving liver function improving effect have.

The above-mentioned hydrous ethanol is a solvent used for the complex extract, and is not particularly limited. For example, the functional ethanol may be a solution in which water and ethanol are mixed at a volume ratio of 1: 0.1 to 1:10. Within this range, it is possible to further improve the effect of improving the hangover and improving the liver function of the health functional food composition containing the compound extract, while improving the active ingredient content of the combined extract.

The health functional food composition may further comprise a food additive together with the complex extract. The kind of the food additive is not particularly limited, but may further include one or more additives such as honey, persimmon vinegar and oligosaccharide. In this case, the taste of the health functional food composition can be enhanced without impairing the flavor of the complex extract, and more advantageous effects can be realized in processing into various food types.

The health functional food composition is not particularly limited, but may be a powder, granule, tablet, capsule, ring, liquid tincture, or processed product in the form of a drink. In this case, the health functional food composition can be advantageously consumed, stored, and distributed, thereby improving the consumer's preference for the product.

The food for which the health functional food composition can be used is not particularly limited and examples thereof include confectionery, sugar, ice cream products, dairy products, meat products, fish products, tofu or glutinous products, edible oils, noodles, , Special nutrition food, health functional food, seasoning food, ice, ginseng products, kimchi pickles, dried bran and other food products. In this case, while satisfying the hangover resolution and hepatic function-improving effects of the health functional food composition, it is possible to satisfactorily satisfy various kinds of foods and effectively supply additional nutritional components.

Another embodiment of the present invention relates to a method for preparing a health functional food composition for improving hangover and liver function as described above.

The method for preparing a health functional food composition for improving hangover and improving liver function comprises the step of preparing a combined extract of radish, goat ginseng, ginger, garlic, pear, bellflower, white raspberry,

The effects of radish, goat ginseng, ginger, garlic, pear, bellflower, white rice, sanjoin, konjin and jujube contained in the above-mentioned complex extract are as described above.

Specifically, it is preferable that 30 wt.% To 70 wt.% Of seedless ginseng, 1 wt.% To 20 wt.% Of ginseng root, 5 wt.% To 35 wt.% Of ginger, 5 wt.% To 25 wt.% Of garlic, 5 wt. Water and 0.5% by weight to 5% by weight, 0.5% to 5% by weight, 0.5% to 5% by weight of acidophilus, 0.5% Ethanol at a volume ratio of 1: 0.1 to 1:10, and extracting the mixture at 20 to 100 DEG C for 1 to 72 hours to prepare a complex extract.

The above-mentioned hydrous ethanol is a solvent used for the complex extract, and is not particularly limited. For example, the functional ethanol may be a solution in which water and ethanol are mixed at a volume ratio of 1: 0.1 to 1:10. Within this range, it is possible to further improve the effect of improving the hangover and improving the liver function of the health functional food composition containing the compound extract, while improving the active ingredient content of the combined extract.

The extraction temperature may be from 20 캜 to 100 캜, more specifically from 60 캜 to 800 캜. Within this range, it is possible to further improve the effect of improving the hangover and improving the liver function of the health functional food composition containing the compound extract, while improving the active ingredient content of the combined extract.

The extraction time may be from 1 hour to 72 hours, more specifically from 2 hours to 24 hours. Within this range, it is possible to further improve the effect of improving the hangover and improving the liver function of the health functional food composition containing the compound extract, while improving the active ingredient content of the combined extract.

The method for preparing a health functional food composition may further comprise adding a food additive to the complex extract to formulate the composition. The kind of the food additive is not particularly limited, but may be, for example, at least one additive of honey, persimmon vinegar and oligosaccharide. In this case, the taste of the health functional food composition can be enhanced without impairing the flavor of the complex extract, and more advantageous effects can be realized in processing into various food types.

The method for preparing the health functional food composition is not particularly limited, but may be a process for processing the health functional food composition into powder, granule, tablet, capsule, ring, liquid tincture or beverage. In this case, the health functional food composition can be advantageously consumed, stored, and distributed, thereby improving the consumer's preference for the product.

The food for which the health functional food composition can be used is not particularly limited and examples thereof include confectionery, sugar, ice cream products, dairy products, meat products, fish products, tofu or glutinous products, edible oils, noodles, , Special nutrition food, health functional food, seasoning food, ice, ginseng products, kimchi pickles, dried bran and other food products. In this case, while satisfying the hangover resolution and hepatic function-improving effects of the health functional food composition, it is possible to satisfactorily satisfy various kinds of foods and effectively supply additional nutritional components.

Example

Hereinafter, the configuration and operation of the present invention will be described in more detail with reference to preferred embodiments of the present invention. It is to be understood, however, that the same is by way of illustration and example only and is not to be construed in a limiting sense.

Example  1: Preparation of complex extract

5% by weight of ginseng, 5% by weight of ginseng, 5% by weight of bellflower, 0.5% by weight of white ginseng, 1% by weight of acidophore, 0.5% Was added 1000 parts by weight of a 50% aqueous ethanol solvent on the basis of 100 parts by weight of the mixture, followed by extraction and filtration at 80 DEG C for about 12 hours to prepare a combined extract.

Comparative Example  1 to 10: Preparation of a single raw material extract

1000 parts by weight of a 50% aqueous ethanol solvent was added to 100 parts by weight of each raw material as shown in Table 1 below, followed by extraction and filtration at 80 ° C for about 12 hours to prepare a single raw material extract of Comparative Examples 1 to 10.

division Raw material Comparative Example 1 radish Comparative Example 2 Goat Comparative Example 3 ginger Comparative Example 4 garlic Comparative Example 5 ship Comparative Example 6 Bellflower Comparative Example 7 A hundred Comparative Example 8 Sanjoin Comparative Example 9 Construction person Comparative Example 10 Jujube

Experimental Example  1. Alcohololytic enzyme ( ADH ) Activity measurement

The activity of ADH, the key enzyme for hangover efficacy, was verified by in vitro experiments.

The activity of ADH was determined by measuring the production rate of NADH produced at the reaction with ethanol, the substrate of the enzyme, at 340 nm. First, 0.14 ml of distilled water, 1.0 M tris-HCl buffer (pH 8.8 ), 0.015 ml of 20 mM NAD ⁺ (Sigma-Aldrich), 0.03 ml of ethanol and 0.01 ml of the sample (final concentration in the reaction solution: 200 μg / ml) and 0.015 ml of ADH (Sigma-Aldrich) and incubated at 30 ° C for 5 minutes. Then, the absorbance was measured at 340 nm for 5 minutes at intervals of 30 seconds. The ADH activity was calculated by the following equation (1). The results are shown in Table 1 and FIG.

[Formula 1]

ADH activity (%) = (absorbance of test group / absorbance of control group) X 100

division ingredient ADH activity (%) Control group Distilled water 100.25 + - 11.52 Example 1 Complex extract 317.26 ± 15.92 Comparative Example 1 Radish extract 267.55 ± 18.12 Comparative Example 2 Goat ginseng extract 227.19 ± 11.76 Comparative Example 3 Ginger extract 182.63 + - 4.38 Comparative Example 4 Garlic extract 122.66 ± 14.81 Comparative Example 5 Pear extract 118.39 + - 19.54 Comparative Example 6 Bellflower extract 143.17 ± 3.27 Comparative Example 7 A white extract 142.02 + - 11.05 Comparative Example 8 Sanjoin extract 166.22 + - 9.15 Comparative Example 9 Extractor 127.41 + - 2.38 Comparative Example 10 Jujube extract 159.88 +/- 16.97

As can be seen from Table 2 and FIG. 1, the activity of Example 1 of the present invention was 317.26 ± 15.92%, which was significantly higher than that of the control, ADH activity.

Experimental Example  2. Acetaldehyde decomposing enzyme ( ALDH ) Active measurement

The activity of ALDH, the key enzyme of hangover efficacy, was verified by in vitro experiments.

The activity of ALDH was measured by measuring the absorbance of NADH in the 340 nm wavelength region using NAD ⁺ as a coenzyme during the acetate formation using acetaldehyde as a substrate.

0.03 ml of 1.0 M Tris-HCl buffer (pH 8.0), 0.01 ml of 20 mM NAD ^+, 0.01 ml of 1.0 M acetaldehyde, 0.01 ml of 3.0 M KCl, 0.01 ml of 0.33 M mercaptoethanol, 0.01 ml of the sample and 0.01 ml of ALDH (Sigma-Aldrich) were dispensed into a 96-well plate and reacted at 30 ° C for 5 minutes. Then, the absorbance was measured at 340 nm for 5 minutes at intervals of 30 seconds. In the control group, no sample was added. The ALDH activity was calculated by the following equation.

[Formula 2]

ALDH activity (%) = (absorbance of experiment / control absorbance) X 100

division ingredient ALDH activity (%) Control group Distilled water 100.62 + - 9.11 Example 1 Complex extract 258.69 ± 21.22 Comparative Example 1 Radish extract 220.26 + - 6.98 Comparative Example 2 Goat ginseng extract 128.95 + - 6.75 Comparative Example 3 Ginger extract 118.02 + - 16.15 Comparative Example 4 Garlic extract 167.33 ± 2.83 Comparative Example 5 Pear extract 169.13 + - 2.81 Comparative Example 6 Bellflower extract 108.84 + 16.35 Comparative Example 7 A white extract 119.47 + 9.27 Comparative Example 8 Sanjoin extract 115.96 ± 1.86 Comparative Example 9 Extractor 148.82 + - 11.16 Comparative Example 10 Jujube extract 142.51 ± 18.72

As can be seen from Table 3 and FIG. 2, the activity of Example 1 of the present invention was 258.69 ± 21.22%, which was significantly higher than that of the control, ALDH activity.

Experimental Example  3. Hangover efficacy simplified clinical trial

In order to measure the hangover resolution efficacy of the composition of the present invention by a simple clinical method, 55 healthy male and female adults aged 20-40 years were tested. The subjects were selected not to drink excessive alcohol within 5 days before the experiment and not to take any other medication. Also, water and other beverages were not consumed from the beginning to the end of the experiment. The subjects were given purified water, Example 1, and Comparative Examples 1 to 10, respectively, 10 minutes after the completion of ingestion of 250 ml of shochu (alcoholic beverage 22 degrees, Chamisil) within 10 minutes, and the alcohol concentration Respectively. The concentration of alcohol in the breath was measured using a portable alcohol breathing apparatus (CASIO, CA2000). In addition, a questionnaire about hanging symptoms was taken 12 hours after alcohol consumption.

division ingredient Alcohol concentration in exhalation (%) Before experiment 0 hours ^* 30 minutes 60 minutes 90 minutes 120 minutes Control group Distilled water 0.00 0.081 0.075 0.071 0.066 0.058 Example 1 Complex extract 0.00 0.082 0.063 0.053 0.042 0.034 Comparative Example 1 Radish extract 0.00 0.081 0.075 0.063 0.051 0.039 Comparative Example 2 Goat ginseng extract 0.00 0.079 0.067 0.061 0.057 0.047 Comparative Example 3 Ginger extract 0.00 0.080 0.072 0.062 0.061 0.049 Comparative Example 4 Garlic extract 0.00 0.084 0.074 0.064 0.059 0.051 Comparative Example 5 Pear extract 0.00 0.076 0.069 0.066 0.061 0.055 Comparative Example 6 Bellflower extract 0.00 0.078 0.072 0.064 0.059 0.052 Comparative Example 7 A white extract 0.00 0.083 0.079 0.072 0.059 0.046 Comparative Example 8 Sanjoin extract 0.00 0.078 0.071 0.065 0.061 0.056 Comparative Example 9 Extractor 0.00 0.073 0.065 0.061 0.056 0.053 Comparative Example 10 Jujube extract 0.00 0.084 0.069 0.062 0.056 0.044

^* 0 hour: Immediately before eating the sample after 10 minutes of alcohol consumption

As can be seen from Table 4 and FIG. 3, Example 1 of the present invention was remarkably superior in reducing the alcohol concentration in the breath group as compared with the control group, And implemented a more improved effect.

Hangover resolution effect (1: None, 2: Slightly available 3: Available, 4: Much available 5: Very good) division headache miscarriage of justice Helplessness thirst Difficulty concentrating Control group Distilled water 2.15 2.91 1.26 1.42 2.11 Example 1 Complex extract 4.28 3.95 4.12 4.66 3.92 Comparative Example 1 Radish extract 3.65 3.81 4.04 4.06 3.28 Comparative Example 2 Goat ginseng extract 3.61 3.55 2.81 2.57 2.91 Comparative Example 3 Ginger extract 2.94 3.57 2.51 1.92 2.34 Comparative Example 4 Garlic extract 2.28 3.20 1.95 2.68 2.61 Comparative Example 5 Pear extract 2.84 2.99 2.61 2.09 2.48 Comparative Example 6 Bellflower extract 2.61 2.97 3.54 2.94 2.48 Comparative Example 7 A white extract 3.19 3.18 3.02 2.48 3.15 Comparative Example 8 Sanjoin extract 3.06 3.28 1.98 1.95 3.66 Comparative Example 9 Extractor 2.82 3.54 3.21 4.28 4.43 Comparative Example 10 Jujube extract 3.57 3.91 4.68 3.11 2.50

As can be seen from Table 5 and FIG. 4, Example 1 of the present invention was remarkably superior in the hangover resolution effect as compared with the control group, and was superior to the single raw material extracts of Comparative Examples 1 to 10 Effect.

Experimental Example  4. Hepatocyte damage protection test in vitro )

The hepatocyte protective effect of the composition of the present invention against oxidative damage by t-BHP (tert-butyl hydroperoxide) was examined in human liver cancer cell line (HepG2).

HepG2 was added to each well of a 96-well plate (200 μl, 2 × 10 ⁴ cells / well) and cultured in a CO ₂ incubator for 24 hours. Each sample was treated and incubated for another 12 hours in a CO ₂ incubator. Twenty microliters of t-BHP (final concentration 1 mM) was added and incubated in a CO ₂ incubator for 5 hours. Cell viability was measured by MTT assay. The MTT (3- (4,5) -dimethylthiazol-2-yl) -2,5-diphenyl-tetrazolium bromide was dissolved in a blue formazan (DMSO) solution at a wavelength of 570 nm by mitochondrial dehydrogenase, an oxidoreductase in the mitochondria of living cells. Absorbing material) is used. Twenty microliters of MTT solution (5 mg / ml in PBS) was added to the 96 well plate and incubated for 2 hours in a CO ₂ incubator. After removing the supernatant, 200 μl of DMSO was added per well and the cell membrane and blue formazan were dissolved for 30 minutes. Absorbance was measured at 570 nm using an ELISA reader and cell viability was measured.

division Cell treatment Cell survival rate % ) Control group Distilled water PBS 100.00 ± 2.65 Negative control group PBS t-BHP 1 mM, 5 hours treatment 28.27 ± 3.25 Example 1 Complex extract 77.45 + - 4.76 Comparative Example 1 Radish extract 59.75 ± 11.64 Comparative Example 2 Goat ginseng extract 50.35 + 9.07 Comparative Example 3 Ginger extract 35.38 ± 10.58 Comparative Example 4 Garlic extract 35.49 + - 6.05 Comparative Example 5 Pear extract 31.85 + 4.69 Comparative Example 6 Bellflower extract 60.18 + - 8.16 Comparative Example 7 A white extract 42.76 + - 2.72 Comparative Example 8 Sanjoin extract 46.07 ± 2.42 Comparative Example 9 Extractor 38.48 + - 4.84 Comparative Example 10 Jujube extract 45.11 ± 7.56

In addition, as shown in Table 6 and FIG. 5, Example 1 of the present invention was remarkably superior in inhibiting the survival of liver cancer cells as compared with the control group, and in addition, the single raw material extracts of Comparative Examples 1 to 10 And implemented a more improved effect.

Experimental Example  5. Ethanol induction Liver damage  Eliminate hangovers in animal models Liver function  Improvement efficacy verification ( in vivo )

Animals Sprague-Dawley male rats (250-300 g) were housed in a constant temperature (22 ± 2 ° C, 55 ± 5% humidity, light / dark cycle, 12 h light and dark cycle) at constant temperature and humidity. Glutamate-pyruvate transaminase (GPT), glutamateoxaloacetate-transaminase (GOT), and a kit for measuring enzyme activity were purchased from Asan Pharmaceutical Co., Ltd. (Korea).

Experimental animals were adapted for one week in a constant temperature and humidity chamber, and then 5 mice were administered to each group. PBS was administered to the control group and 40% ethanol was administered at 5 g / kg body weight in the experimental group. After 30 minutes, each sample was orally administered at 500 mg / kg body weight. In order to measure the concentration of ethanol and acetaldehyde in blood by time, blood was collected from the American artery in anesthetized with ether at 1, 3, and 5 hours after the alcohol administration. The collected blood was left at room temperature for 10 minutes and the serum was separated using a centrifuge. Serum was measured at 24 hours after sacrifice and blood sampling and serum separation were performed in the aorta.

The concentration of ethanol in the blood was measured by reacting 10 μl of serum with 3 ml of NAD-ADH solution at 30 ° C for 10 minutes using a measuring kit (Sigma Chemical Co., USA) and measuring the absorbance at 340 nm wavelength of the generated NADH.

Blood ethanol concentration (mg / ml) Elapsed time 1 hours 3 hours 5 hours Control group PBS 40% ethanol 5 g / kg body weight Oral administration 1.21 0.26 1.02 + - 0.11 0.68 + 0.14 Example 1 Complex extract 0.91 ± 0.19 0.69 ± 0.28 0.31 + 0.18 Comparative Example 1 Radish extract 1.12 + 0.06 0.98 ± 0.06 0.53 + - 0.24 Comparative Example 2 Goat ginseng extract 1.15 ± 0.11 0.93 + - 0.34 0.66 + 0.22 Comparative Example 3 Ginger extract 1.06 + 0.08 0.88 0.28 0.58 ± 0.15 Comparative Example 4 Garlic extract 1.18 ± 0.02 0.98 ± 0.16 0.46 ± 0.06 Comparative Example 5 Pear extract 1.11 ± 0.09 0.81 ± 0.01 0.41 ± 0.06 Comparative Example 6 Bellflower extract 0.95 + 0.37 0.77 + 0.14 0.39 ± 0.08 Comparative Example 7 A white extract 1.07 + - 0.21 0.84 + 0.24 0.44 + 0.02 Comparative Example 8 Sanjoin extract 0.99 ± 0.09 0.83 ± 0.09 0.59 + 0.13 Comparative Example 9 Extractor 1.13 ± 0.18 0.93 + - 0.05 0.55 + 0.17 Comparative Example 10 Jujube extract 1.04 + - 0.14 0.81 + 0.06 0.55 + 0.21

As can be seen from Table 7 and FIG. 6, Example 1 of the present invention was remarkably superior in reducing ethanol concentration in blood as compared with the control group, and was superior to Comparative Example 1 to Comparative Example 10 which is a single raw material extract Effect.

The blood acetaldehyde concentration was determined by measuring the absorbance at 340 nm using an aldehyde assay kit (Megazyme, Ireland).

Blood Acetaldehyde  Concentration (mg / ml) Elapsed time 1 hours 3 hours 5 hours Control group PBS 40% ethanol 5 g / kg body weight Oral administration 0.94 + 0.06 0.71 ± 0.05 0.53 + 0.04 Example 1 Complex extract 0.77 + 0.02 0.59 + 0.06 0.32 ± 0.05 Comparative Example 1 Radish extract 0.79 + 0.11 0.71 ± 0.08 0.44 ± 0.16 Comparative Example 2 Goat ginseng extract 0.78 ± 0.15 0.61 ± 0.15 0.47 ± 0.08 Comparative Example 3 Ginger extract 0.87 ± 0.08 0.66 + 0.11 0.39 + 0.17 Comparative Example 4 Garlic extract 0.81 ± 0.05 0.68 ± 0.16 0.43 + 0.11 Comparative Example 5 Pear extract 0.94 + - 0.05 0.77 ± 0.09 0.49 + 0.11 Comparative Example 6 Bellflower extract 0.88 ± 0.06 0.69 ± 0.05 0.39 + 0.15 Comparative Example 7 A white extract 0.79 ± 0.09 0.72 + 0.06 0.41 + 0.18 Comparative Example 8 Sanjoin extract 0.87 ± 0.02 0.66 + - 0.05 0.48 ± 0.08 Comparative Example 9 Extractor 0.80 + 0.14 0.68 ± 0.08 0.41 + - 0.05 Comparative Example 10 Jujube extract 0.83 0.21 0.63 + - 0.01 0.39 ± 0.06

As can be seen from Table 8 and FIG. 7, Example 1 of the present invention was remarkably superior in reducing the acetaldehyde concentration in blood as compared with the control group, and was superior to Comparative Example 1 to Comparative Example 10 And implemented an improved effect.

Blood GOT and GPT values were obtained from Asan Pharm. In other words, 1 ml of each substrate solution was stabilized at 37 ° C for 5 minutes, 0.2 ml of serum was added, and GOT was reacted for 60 minutes and GPT was reacted for 30 minutes. 1 ml of a colorimetric solution (2, 4-dinitrophenylhydrazine) was added and reacted at room temperature for 20 minutes. The reaction was stopped with 10 ml of 0.4 N NaOH solution and the absorbance was measured using an ELISA reader with distilled water at a wavelength of 505 nm as a control.

GOT in serum (IU / L) sham PBS PBS administration 12.64 ± 3.18 (-) control PBS 40% ethanol 5 g / kg body weight Oral administration 81.92 + - 11.28 Example 1 Complex extract 32.68 + - 6.91 Comparative Example 1 Radish extract 64.92 + 1.54 Comparative Example 2 Goat ginseng extract 69.54 + - 6.84 Comparative Example 3 Ginger extract 57.68 + - 6.99 Comparative Example 4 Garlic extract 66.95 + - 3.94 Comparative Example 5 Pear extract 48.36 + - 3.55 Comparative Example 6 Bellflower extract 58.19 ± 1.98 Comparative Example 7 A white extract 67.62 ± 14.67 Comparative Example 8 Sanjoin extract 49.68 ± 5.92 Comparative Example 9 Extractor 58.47 + - 8.16 Comparative Example 10 Jujube extract 52.94 + - 2.26

GPT in serum (IU / L) sham PBS PBS administration 8.33 ± 5.99 (-) control PBS 40% ethanol 5 g / kg body weight Oral administration 72.94 ± 5.19 Example 1 Complex extract 38.61 + - 3.97 Comparative Example 1 Radish extract 62.99 + - 3.77 Comparative Example 2 Goat ginseng extract 58.67 ± 10.62 Comparative Example 3 Ginger extract 48.62 + - 8.91 Comparative Example 4 Garlic extract 53.38 + - 2.72 Comparative Example 5 Pear extract 44.91 + - 3.31 Comparative Example 6 Bellflower extract 47.82 + - 2.64 Comparative Example 7 A white extract 58.67 ± 5.66 Comparative Example 8 Sanjoin extract 46.82 + - 7.19 Comparative Example 9 Extractor 68.19 + - 2.68 Comparative Example 10 Jujube extract 52.92 + - 4.36

As can be seen from Tables 9 and 10 and FIGS. 8 and 9, Example 1 of the present invention showed remarkably superior inhibitory effect on blood GOP and GPT activity as compared with the control group, To 10 < th >

Claims (5)

It contains complex extract of radish, goat ginseng, ginger, garlic, pear, bellflower, white,
Wherein the combined extract comprises 30 wt.% To 70 wt.% Of no extract, 1 wt.% To 20 wt.% Ginseng, 5 wt.% To 35 wt.% Ginger, 5 wt.% To 25 wt.% Garlic, 5 wt. Wherein the function of the mixture comprising 5% to 30% by weight, 0.5% to 5% by weight of white rice, 0.5% to 5% by weight of acidophore, 0.5% to 5% A health functional food composition for improving hangover and liver function, which is an ethanol extract.
delete The method according to claim 1,
Wherein the health functional food composition further comprises one or more additives selected from the group consisting of honey, persimmon vinegar, and oligosaccharides.
The method according to claim 1,
Wherein said health functional food is powder, granule, tablet, capsule, ring, liquid tincture or beverage in the form of solution of hangover and improvement of liver function.
, 5% to 25% by weight of garlic, 5% by weight to 30% by weight of pear, 5% by weight of platycodon, Water and ethanol were added to a mixture comprising 30 wt.%, 0.5 wt.% To 5 wt.% Of an acid, 0.5 wt.% To 5 wt.% Of an acid acceptor, 0.5 wt.% To 5 wt. 1 to 0.1: 1 to 1: 10 by volume, and then extracting the extract at 20 to 100 DEG C for 1 to 72 hours to prepare a complex extract. The composition for health functional food composition for improving hangover and improving liver function Way.

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