KR20140041187A - Anti-cancer composition containing erythronium japonicum extract - Google Patents
Anti-cancer composition containing erythronium japonicum extract Download PDFInfo
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- KR20140041187A KR20140041187A KR1020120108236A KR20120108236A KR20140041187A KR 20140041187 A KR20140041187 A KR 20140041187A KR 1020120108236 A KR1020120108236 A KR 1020120108236A KR 20120108236 A KR20120108236 A KR 20120108236A KR 20140041187 A KR20140041187 A KR 20140041187A
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- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/308—Foods, ingredients or supplements having a functional effect on health having an effect on cancer prevention
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- Alternative & Traditional Medicine (AREA)
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- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicines Containing Plant Substances (AREA)
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Abstract
Description
The present invention relates to a pharmaceutical composition comprising a plant extract having an anticancer effect as an active ingredient.
Cancer is characterized by uncontrolled cell growth, and these abnormal cell growths form cell masses called tumors that can invade and destroy new surrounding tissues and organs, creating new growth sites. Collectively, a group of diseases that can take away.
These cancers are one of the incurable diseases that humanity has to solve. Despite the enormous amount of capital invested in the development to cure them all over the world, and the advancement of medical technology, the incidence and death of cancer continues to increase. There is a trend. According to the latest statistics of the World Health Organization, more than 10 million new cancer patients are reported worldwide each year.In Korea, cancer is the highest cause of death and more than 100,000 people are diagnosed annually. He is killed by this cancer.
There are many kinds of cancer causing factors, such as internal factors such as genetic factors or immunological factors, and external factors such as chemicals, radiation, or viruses. In particular, in relation to the recent increase in the number of cancer patients, it is known that the cause of cancer in modern society accounts for a greater proportion of the environmental factors than the genetic factors. As it is known to be very high, the increase in the incidence of cancer and the death toll by cancer is expected to continue unless the living environment in the modern society is fundamentally changed. In particular, if cancer is not detected early, it is almost impossible to cure it. As the disease progresses, the pain and economic loss of the patient and family are enormous, and the social cost such as financial depletion of medical insurance is incurred. The importance of prevention and early treatment is increasingly emphasized.
Recently, new cancer therapeutics are being developed through research on the occurrence and metastasis of cancer, the physiology of cancer cells, the diagnosis and treatment of cancer, and the search for substances having anti-cancer effects including food. In this regard, since many side effects are caused by strong cytotoxicity of chemicals used in conventional chemotherapy, researches are being made to develop anticancer agents from natural products, especially plants or plant herbs.
In order to meet the above demands, an object of the present invention is to provide a natural product-derived anticancer composition excellent in cancer cell killing effect.
In order to achieve the above object, the present invention provides an anticancer composition comprising a natural product, that is, a plant-derived active ingredient. More specifically, the present invention provides an anticancer composition comprising an extract of Ezle as an active ingredient.
In addition, the present invention may be a composition for the treatment or prevention of cancer, including the extract of the extract as an active ingredient. In addition, the present invention may be a food composition for cancer improvement or prevention, including the extract of the extract as an active ingredient.
The inventors of the present invention ascertained that the extract was cancer cell killing effect of MTT analysis using HepG2 cells, which are human liver cancer-derived cells, and particularly, butanol fraction of the butanol fraction in the fraction is very excellent, On the basis of this, the present invention has been completed.
Hereinafter, the present invention will be described in more detail.
The present invention relates to an anticancer composition comprising the extract as an active ingredient.
Dog-tooth Violet, Erythronium japonicum ) is a perennial herb belonging to the family Liliaceae, also known as lobster. The algae grow mainly in fertile lands of high lands, grow in valleys, and are distributed in Korea and Japan. The leaf of the algae is egg-shaped or oval with purple pattern on the green background and the edge is flat, and the leaf body is long oval, the petal is lanceolate, 6, curled and purple, 6 stamens and 1 pistil. . In addition, the fruit of the erynggi fruit in July-August is a wide oval or spherical shape with a capsule. Although the algae is a wildflower, it has recently been spotlighted for ornamental use, and it has been known to relieve vomiting and diarrhea because it has an effect of improving gastrointestinal function in folk medicine, and leaves have been edible as herbs.
The Alzheimer extract may be prepared according to a conventional method of preparing a plant extract, for example, an extract solvent is added to the Alzheimer's flower, leaves, branches, roots, fruits or bark or a pulverized product thereof, preferably the Alzheimer's leaf. It may be prepared by fractionation by adding a fractional solvent to the crude extract prepared by preparing or extracted with an extraction solvent.
The extraction solvent may be at least one selected from the group consisting of water and an organic solvent. The organic solvent may be a polar solvent such as alcohol having 1 to 5 carbon atoms, the alcohol dilution water, ethyl acetate or acetone and a nonpolar solvent of ether, chloroform, benzene, hexane or dichloromethane, or a mixed solvent thereof. The alcohol having 1 to 5 carbon atoms may be methanol, ethanol, propanol, butanol, or isopropanol, but is not limited thereto. In addition, the alcohol dilution water may be an alcohol diluted with water at 50 to 99.9% (v / v).
Extraction solvent of the extract of the present invention is preferably at least one selected from the group consisting of water, alcohol having 1 to 5 carbon atoms, alcohol dilution water and mixtures thereof, more preferably water, having 1 to 4 carbon atoms It may be any one selected from the group consisting of alcohols and mixtures thereof, even more preferably may be 75% to 99% aqueous ethanol or 75% to 99% aqueous methanol solution. For example, the extraction process may be performed at 4 ° C. to 120 ° C., or 50 ° C. to 90 ° C., or 60 ° C. to 80 ° C., but is not limited thereto. The extraction time is not particularly limited, but may be 10 minutes to 12 hours.
The extract of the present invention may be prepared according to a conventional method of producing a plant extract, specifically, may be cold extraction method, hot extraction method, heat extraction method, ultrasonic extraction method, etc., a conventional extraction device, ultrasonic grinding extractor or fractionator It is available.
In addition, the extract extracted with the solvent is then any one solvent selected from the group consisting of hexane, methylene chloride, acetone, ethyl acetate, ethyl ether, chloroform, water and mixtures thereof, preferably butanol, chloroform and ethyl acetate The fractionation process may be further carried out with one or more solvents selected from the group consisting of one or more solvents, more preferably butanol selected from the group consisting of butanol and chloroform. Two or more kinds of solvents may be used for the fractionation, and the solvent extracts may be sequentially used or mixed according to the polarity of the solvent. In addition, the fractionation process may be performed at 4 ° C. to 120 ° C., or 50 ° C. to 90 ° C., or 60 ° C. to 80 ° C., but is not limited thereto.
The thus-prepared extract or the fraction obtained by performing the fractionation process may be filtered, concentrated or dried to remove the solvent, and may be subjected to both filtration, concentration and drying. Specifically, the filtration can be performed using a filter paper or a vacuum filter, and the concentration can be reduced by using a vacuum concentrator, for example, a rotary evaporator. The drying can be performed by, for example, freeze drying.
Extract of methanol solution of the leaf of the present invention MTT analysis of cancer cells HepG2, when treated with more than 250 ㎍ / ㎖ has a cancer cell killing effect on about 90% or more of cancer cells before treatment, the 80% methanol aqueous solution Compared with the extract, butanol, chloroform or ethyl acetate fractions have better killing effects, and especially fractions fractionated with butanol have a cancer cell killing effect on more than 90% of cancer cells even when 125 ㎍ / ml is treated. It was confirmed to have.
Alejandro extract, an active ingredient of the anticancer composition, is not only excellent in anticancer activity, but is prepared using natural plants that can be used for food, so side effects are not a problem, and safety is excellent, and thus the anticancer composition is used to treat cancer. Or as a pharmaceutical composition for prevention or as a food composition for improving or preventing cancer.
The anticancer composition of the present invention, in particular, the anticancer composition or the composition for treating or preventing cancer containing the extract as an active ingredient containing the extract as an active ingredient may include the extract alone as an active ingredient, other formulations It may further comprise a pharmaceutically acceptable carrier, excipient or diluent depending on the method of use and purpose of use.
In the present invention, cancer is also called a malignant tumor, grows rapidly while infiltrating surrounding tissues into abnormally grown masses by autonomous overgrowth of body tissues, and spreads or spreads to various parts of the body and threatens life. Means a disease to be caused, the cancer includes carcinoma and sarcoma, for example breast cancer, stomach cancer, liver cancer, lung cancer, colon cancer, cervical cancer, endometrial cancer, cerebrospinal tumor, head and neck cancer, thymoma , Esophageal cancer, pancreatic cancer, kidney cancer, bladder cancer, prostate cancer, testicular cancer, germ cell tumor, ovarian cancer, lymphoma, acute leukemia, chronic leukemia and multiple myeloma.
The composition for treating or preventing cancer can be directly applied to animals including humans. The animal is a group of organisms corresponding to plants. It mainly consumes organic matter as nutrients, and differentiates digestive organs, excretory or respiratory organs. Preferably, it is a mammal, more preferably a human.
The anticancer composition or the extract may be used alone in the composition for treating or preventing cancer, and may further include other pharmacologically acceptable carriers, excipients, diluents or subcomponents. More specifically, when the anti-cancer composition or the composition comprising the ulge extract is used as a medicament, or for medicinal or pharmaceutical use, the anti-cancer composition or the ulge extract is pharmaceutically acceptable according to a conventional method It can be used mixed with a carrier or excipient or diluted with a diluent.
In this case, the content of the anticancer composition or the extract in the aging may be 0.001% by weight to 99.9% by weight, 0.1% by weight to 99% by weight or 1% by weight to 20% by weight, but is not limited thereto. Depending on the mode of use and the method of use, the content of the anticancer composition or the extract may be appropriately adjusted to a pharmaceutically effective amount or a preferred amount.
The term "pharmaceutically acceptable" as used herein means physiologically acceptable and does not normally cause an allergic reaction such as gastrointestinal disorder, dizziness, or the like when administered to an animal, preferably a human. The pharmaceutically effective amount may be appropriately changed depending on the disease and its severity, the age, body weight, health condition, sex, administration route and treatment period of the patient.
Examples of the pharmaceutically acceptable carrier, excipient or diluent include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, Cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil, propylhydroxybenzoate, talc, magnesium stearate and mineral oil , Dextrin, calcium carbonate, propylene glycol, liquid paraffin, and physiological saline. However, the present invention is not limited thereto, and any conventional carrier, excipient or diluent may be used. In addition, the composition for treating or preventing cancer may be a conventional filler, an extender, a binder, a disintegrant, an anticoagulant, a lubricant, a wetting agent, a pH adjuster, a nutrient, a vitamin, an electrolyte, alginic acid and its salt, Colloids, glycerin, fragrances, emulsifiers or preservatives, and the like. The components may be added independently or in combination with the anticancer composition or the erling extract.
In addition, the composition of the present invention may further comprise a substance used as a known anticancer agent in addition to the above-mentioned effective ingredient.
When the composition for treating or preventing cancer is used as a medicament, the administration method can be oral or parenteral, and examples thereof may be administered through various routes including oral, transdermal, subcutaneous, intravenous or muscular.
In addition, the formulations of the compositions for treating or preventing cancer may be varied depending on the method of use and may be formulated so as to provide rapid, sustained or delayed release of the active ingredient after administration to the mammal, ≪ / RTI > In general, solid dosage forms for oral administration include tablets, pills, soft or hard capsules, pills, powders and granules, which may contain one or more Excipients such as starch, calcium carbonate, sucrose or lactose, gelatin, and the like. In addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. Liquid preparations for oral use include suspensions, solutions, emulsions, and syrups. In addition to water and liquid paraffin, which are commonly used simple diluents, various excipients such as wetting agents, Sweeteners, fragrances, preservatives, and the like.
Forms for parenteral administration are CREAM, LOTIONS, ONITMENTS, PLASTERS, LIQUIDS AND SOULTIONS, AEROSOLS, FRUIDEXTRACTS, Elixir (ELIXIR), INFUSIONS, SACHET, PATCH or INJECTIONS.
Furthermore, the compositions of the present invention may be formulated using any suitable method known in the art or using methods disclosed in Remington's Pharmaceutical Science (recent edition), Mack Publishing Company, Easton PA .
The dosage of the composition can be determined in consideration of the administration method, the age, sex, the severity of the patient, the condition of the patient, the degree of absorption of the active ingredient in the body, the inactivation rate and the drug to be used together. Kg body weight to 500 mg / kg body weight, 0.1 mg / kg body weight to 400 mg / kg body weight or 1 mg / kg body weight to 300 mg / kg body weight, And may be administered once or several times in divided doses. The dose is not intended to limit the scope of the invention in any way.
In addition, the present invention provides a food composition for cancer improvement or prevention, comprising the anticancer composition or the extract of the present invention as an active ingredient. The cancer improving or preventing food composition comprising the anti-cancer composition or the extract of the present invention as an active ingredient may further include appropriate carriers, excipients and diluents commonly used in the preparation thereof.
The algae extract is an algae leaf alcohol aqueous solution extract, specifically, the algae leaf alcohol aqueous solution extract or a fraction fractionated with at least one solvent selected from the group consisting of butanol, chloroform and ethyl acetate of the extract, preferably butanol of the extract Fractions.
Examples of the food composition of the present invention include food, food additives, beverages or beverage additives.
As used herein, the term " food " means a natural product or a processed product containing one or more nutrients. Preferably, it means that the food can be directly eaten through a certain degree of processing. It is intended to include food, food additives, health functional foods and beverages.
Examples of foods that may include the anticancer composition or the extract may include various foods, beverages, gums, teas, vitamin complexes, and functional foods. In addition, the food in the present invention includes special nutritional products (e.g., crude oil, infant food, baby food, etc.), processed meat products, fish products, tofu, jelly, noodles (e.g., ramen, noodles, etc.), health supplements, seasoned foods (E.g., soy sauce, miso, red pepper paste, mixed soy sauce), sauces, confectionery (e.g. snacks), dairy products (e.g. fermented milk, cheese, etc.), other processed foods, kimchi, pickles (various kimchi, pickles, etc.), beverages (Eg, fruits, vegetable drinks, soy milk, fermented beverages, ice creams, etc.), natural seasonings, vitamin complexes, alcoholic beverages, alcoholic beverages and other health supplements. The food, beverage or food additive may be prepared by a conventional production method.
In the present invention, the functional food refers to a food group which is imparted with added value to function and express the function of the food by using physical, biochemical, biotechnological techniques and the like, the regulation of the biological defense rhythm of the food composition, Means a food which is processed and designed so that the body control function regarding the body is sufficiently expressed to the living body. The functional food may include a food-acceptable food-aid additive, and may further comprise suitable carriers, excipients and diluents conventionally used in the production of functional foods.
In the present invention, beverage is a generic term for drinking or enjoying a taste, and is intended to include functional beverages. The beverage contains the anticancer composition or the ergot extract as an active ingredient as an essential ingredient in an indicated ratio, and there are no special limitations to the other ingredients, and it contains various flavors or natural carbohydrates as additional ingredients, such as ordinary drinks. can do. Examples of such natural carbohydrates include monosaccharides such as glucose, fructose and other disaccharides such as maltose, sucrose and the like and polysaccharides such as dextrin, cyclodextrin and the like, and Sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (tauumatin, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The ratio of the natural carbohydrate may generally be about 1 g to 20 g, preferably 5 g to 12 g per 100 ml of the food composition of the present invention. It may further contain pulp for the production of beverages, vegetable beverages.
In addition to the above-mentioned composition, the composition of the present invention can be used as a flavoring agent such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, coloring agents and aggravating agents (cheese, chocolate etc.), pectic acid and its salts, Salts, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated beverages and the like. These components can be used independently or in combination. The additive may be from 0 to 20 parts by weight, preferably 0.00001 to 15 parts by weight, based on 100 parts by weight of the anticancer composition or the extract of the present invention, but is not limited thereto.
Functional beverage in the present invention is a biological defense rhythm control, disease prevention and the like having a beverage group or a beverage composition that has added value to the beverage by using physical, biochemical, biotechnological techniques, etc. to function and express the function of the beverage to a specific purpose It means a beverage that is designed and processed to fully express the function of the gymnastics for recovery.
The functional beverage is not particularly limited to other ingredients except for containing the anticancer composition of the present invention or the ergot extract as essential ingredients in the indicated ratios, and contains various flavors or natural carbohydrates as additional ingredients, such as ordinary drinks. can do. Examples of such natural carbohydrates include monosaccharides such as glucose, fructose and other disaccharides such as maltose, sucrose and the like and polysaccharides such as dextrin, cyclodextrin and the like, and xylitol Sugar alcohols such as sorbitol and erythritol. Natural flavors (tau martin, stevia extract (e.g., rebaudioside A, glycyrrhizin, etc.) and synthetic flavors (saccharin, aspartame, etc.) can be advantageously used as flavors other than those described above The natural carbohydrate may be included in an amount of 0 to 20 parts by weight, preferably 1 to 18 parts by weight, more preferably 5 to 12 parts by weight per 100 parts by weight of the composition of the present invention.
In addition, in the cancer improving or preventing food composition comprising the anti-cancer composition or the extract extract as an active ingredient, the amount of the anti-cancer composition or the extract extract may be included as 0.00001% to 50% by weight of the total food weight, The beverage composition may be included in a ratio of 0.001 g to 50 g, preferably 0.01 g to 10 g, based on 100 ml of the total volume of the food, but is not limited thereto.
The extract of the present invention has a cancer cell killing effect as a result of MTT analysis of cancer cells, safety of the plant as a conventionally used as food extract is not only recognized as a safety, but also no side effects, treatment, improvement of various cancers, including liver cancer And can be used in many ways for prevention.
1 is a flow chart showing the manufacturing process of the extract and fractions, according to an embodiment of the present invention.
Figure 2 is a graph showing the results of the anticancer effect of the extract was measured by MTT assay using HepG2 cell line, a liver cancer cell line, according to an embodiment of the present invention, wherein each value in the graph is 80% methanol aqueous solution The addition amount of the extract is shown, and the numerical value on the vertical axis indicates the cell viability as a relative value.
Figure 3 is a graph showing the results of the anticancer effect of the extract and fractions of the extract using the liver cancer cell line HepG2 cell line according to an embodiment of the present invention, MTT assay method, the horizontal axis of the graph is the extraction solvent or fraction Solvents are shown, and each figure in the graph represents the addition amount of the extract and fractions, and the numerical value in the vertical axis of the graph represents the cell viability as a relative value.
Hereinafter, the present invention will be described in detail with reference to Examples. However, the following examples are merely illustrative of the present invention and can be modified in many different forms, which are intended to illustrate the present invention, but the scope of the present invention is not limited by the following examples.
<Example 1> Preparation of the extract
The leaves of Erythronium japonicum , a native plant of Suncheon Jogyesan, collected in 2011, were lyophilized at -20 ° C and powdered. As shown in FIG. 1, the powdered erex extract and fractions were prepared by immersing 300 g of the erase powder sample in 3 L of an 80% methanol aqueous solution, followed by extracting with a warm immersion method, and sequentially extracting an organic solvent. Treatment gave a fraction.
More specifically, 3L of 80% methanol aqueous solution was added to each 300 g of the leaves, flowers, stems, and roots, and each of the leaves, flowers, stems, and roots was completely immersed in the extraction solvent, and then refluxed at 70 ° C. for 24 hours. Extracted during. The extract was prepared by filtration of the extract of each part prepared through the extraction, and the filtrate was concentrated under reduced pressure using a vacuum concentrator, and freeze-dried at -20 ° C to obtain a powder.
Hexane, water (H 2 O) and methanol (Methanol) was added to the mixture of 80% methanol extract prepared by mixing 10: 9.5: 0.5 based on the volume of about 3 L of the mixed solvent After shaking from side to side, the layers were allowed to separate. After the layers were separated by the standing, the hexane fraction was prepared by separating the hexane fractionation layer, which is the upper layer of the separated layer, and the hexane fractionation layer, in the aqueous layer, by hand. The chloroform fraction was prepared by separating the hexane layer and adding the same amount of chloroform (CHCl 3 ) to the remaining solution (water layer) and fractionating to separate only the chloroform layer, and separating the chloroform layer and remaining solution (water layer). Ethyl acetate (EtOAc) was added to the same amount and fractionated to prepare an ethyl acetate fraction by separating only the ethyl acetate layer. The ethyl acetate layer was separated and the same amount of butanol (BuOH) was added to the remaining solution (water layer). The butanol fraction was prepared by adding and fractionating to separate only the butanol layer, and the remaining solution was used as the water fraction.
The solvent fraction for each solvent prepared through the fractionation was concentrated under reduced pressure using a vacuum concentrator, and then freeze-dried at -20 ° C to prepare a fraction. The prepared extracts and fractions are shown in Table 1 below.
The extracts and fractions prepared above were stored at -20 ° C. until they were used for the experiment, and each extract and fraction was dissolved in DMSO for use in the experiment.
<Example 2> Anti-cancer effect measurement-cancer cell death effect
In order to measure the anticancer effect of the Eji extract prepared in Example 1, HepG2 cells were derived from hepatic cancer cells, human liver cancer cells. The HepG2 cells were cultured in medium using DMEM / F12 (Dulbecco's modified Eagle's medium / Nutrient Mixture Ham's F12) and FBS (fetal bovine serum).
The cultured HepG2 cells were aliquoted into 24 well-plates at a density of 50,000 cells / well and incubated for 24 hours. After the incubation, the control or untreated control and various concentrations of the extract or fraction of Example 1 was added and incubated for 6 hours, followed by MTT assay. Specifically, the MTT assay was added to the extract or fraction of Example 1 and incubated for 6 hours in each well of 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium dissolved in PBS buffer. Add 25 μl of bromide (MTT) solution and incubate for another 4 hours, remove supernatant so that the formazan formed on the bottom of the well is not scattered, and dissolve in 100 μl of DMSO at 540 nm with ELIZA microplate reader (molecula Devices, SunnyvaleCA). Absorbance was measured by the method.
As shown in FIG. 2, it was confirmed that cancer cell killing effect was weak in the experimental group treated with the extract of 125 g / ㎖, but significant cancer cell killing effect in the experimental group treated with 250 ㎍ / ㎖. From the above results, it was confirmed that the extract of the aqueous methanol solution of the present invention has significant cancer cell killing effect even at 250 µg / ml. In addition, as shown in Figure 3, in the experimental group treated with 125 ㎍ / ㎖ extract, it was confirmed that the butanol fraction has a remarkably excellent cancer cell killing effect, even in the case of the chloroform or ethyl acetate fraction extract methanol solution or other The anticancer effect was confirmed to be superior to the fraction.
Claims (5)
The oleji extract is an anticancer composition of the oleage leaf is extracted from at least one selected from the group consisting of water, alcohol having 1 to 5 carbon atoms and mixtures thereof with an extraction solvent.
The oleji extract is an anticancer composition in which one or more selected from the group consisting of butanol, chloroform and ethyl acetate in the oleore leaf alcohol aqueous solution extract.
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN104130338A (en) * | 2014-08-06 | 2014-11-05 | 新疆医科大学 | Preparation method of erythronium sibiricum polysaccharide and application of erythronium sibiricum |
CN108096444A (en) * | 2018-02-05 | 2018-06-01 | 邱小兵 | A kind of drug for treating tumour and preparation method thereof |
CN112274588A (en) * | 2019-07-24 | 2021-01-29 | 中国医学科学院药物研究所 | Preparation method and application of extract of plant of genus Convolvulus |
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
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CN104130338A (en) * | 2014-08-06 | 2014-11-05 | 新疆医科大学 | Preparation method of erythronium sibiricum polysaccharide and application of erythronium sibiricum |
CN104130338B (en) * | 2014-08-06 | 2016-11-16 | 新疆医科大学 | The preparation method and applications of Buick gracilis polysaccharide |
CN108096444A (en) * | 2018-02-05 | 2018-06-01 | 邱小兵 | A kind of drug for treating tumour and preparation method thereof |
CN112274588A (en) * | 2019-07-24 | 2021-01-29 | 中国医学科学院药物研究所 | Preparation method and application of extract of plant of genus Convolvulus |
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