KR101062172B1 - Anti-cancer health food containing mushroom fermented ginseng powder - Google Patents

Abstract

The present invention relates to a health food for cancer containing mushroom fermented ginseng powder, comprising: drying ginseng; Sterilizing the ginseng; Inoculating and fermenting mushroom seedlings on the ginseng to make fermented products; Pulverizing the fermented product. As a result, fermented ginseng is more effective in anticancer action and lowering blood sugar by fermenting conventional ginseng and mushroom spawn, inhibiting cancer cell neogenesis, inducing suicide of cancer cells, and inhibiting metastasis.

Mushroom spawn, ginseng, fermented ginseng, saponin, anticancer activity

Description

Cancer health foods including fermented Ginseng with mushroom}

The present invention relates to a health food for cancer containing mushroom fermented ginseng powder.

Malignant tumor, the worst disease that threatens human health, or cancer, is the most common cause of death in Western society after cardiovascular disease. In modern society, lung cancer due to the aging smoking population and air pollution has increased significantly, and colon cancer, breast cancer, and prostate cancer have been increased due to the consumption of high-fat diet and the increase of drinking water of environmental pollutants. It is steadily increasing.

Therefore, there is an urgent need for the creation of anticancer substances that can contribute to the promotion of human health, the improvement of healthy quality of life and the promotion of human health by enabling early prevention and treatment of cancer. Drugs developed for the treatment of cancer have low specificity for cancer cells due to the nonspecificity acting systemically along the bloodstream following systemic injection or oral administration. Therefore, there are many systemic side effects due to the administration of the drug, and there are disadvantages that many side effects appear compared to surgery or radiation therapy. The cancer treatment period using the existing anticancer drugs is caused by various anticancer drug side effects by discontinuing treatment by administering 3-5 days a week for 6 months to 1 year at intervals of 3-4 weeks.

Existing chemical therapies that directly attack cancer cells to exert their effects are not only very strong side effects but despite the nonspecificity disadvantages are limited clinically. Many inventors have been looking for a natural material having anticancer activity, and among them, many researches on the anticancer activity of mushrooms including ginseng have been reported.

Among the natural products, ginseng is a perennial plant belonging to the genus Panax, which has been used as a traditional drug in Korea and China since ancient times, and the genus Panax is one of the most important genus in Chinese medicine. Ginseng not only has an excellent effect on the prevention and treatment of various diseases, but also gives strength and vitality to those who have no disease at all.

Ginseng is used as a medicine to intensify the yang of soy sauce, heart, lung, kidney, and spleen, and open stomach of stomach, stop ovulation, vomiting, thirst and make blood flow well. It is lighter and longer life. In addition, saponin, the main component of ginseng, is known to be good for cancer, diabetes, male function enhancement, hypertension, arteriosclerosis, dementia, osteoporosis prevention, anti-aging, promoting brain activity, menopausal disorders, skin care for women. In particular, it has been found to have higher anticancer activity, potent hypoglycemic action, and suppress complications than anticancer drugs.

The chemical structure of saponin, the main component of ginseng, is a glycoside consisting of glyco and aglycone. When these saponins are absorbed by the body, they are broken down by the unique microorganisms in the human intestine and absorbed into the body. However, the intestinal microorganisms that break down ginseng saponin vary depending on the human constitution and dietary habits, and their presence and degree of retention. Therefore, the effects of ginseng saponin may be different after taking ginseng.

Chaga ( Inonotus obliquus ) is a parasitic mushroom that grows on birch trees in more than 45 degrees of latitude, such as Siberia, North America, and Northern Europe, and is effective in treating adult diseases such as cancer. It grows on the sap and eats the sap, which usually grows for 15 to 20 years, and is also found in alders, willows, and maples, but these are ineffective. In Russia, it has been handed down as a elixir for treating incurable diseases since the 16th century. In 1951, it began to be studied at the Soviet Academy of Sciences, Komarov Science Institute, and is now recognized as an official medicine for cancer treatment in Russia.

Ganoderma lucidum is also called Bulchocho. It occurs in the roots of hardwoods in summer and also sprouts on the ground. It is a year-round mushroom that has a gloss like lacquer on its lampshade and bag surface. In oriental medicine, it is effective in tonic, Jinhae, and small sarcoma. These mushrooms are covered with hard skin and have a varnish like varnish. They are used as an herbal medicine in Japan and as a Chinese herbaceous land, and are widely used for decorative purposes.

Coriolus versicolor is called cloud cloud, mushroom paper or cloud finger because it is formed as a cloud. Or it is called 'cloud mushroom'. It grows on rotten stems and branches of hardwoods from spring to autumn. Fruiting bodies are engraftment, eccentric, or layered together. It is effective for the prevention and treatment of natural hepatitis, chronic active hepatitis, chronic bronchitis, liver cancer, gastrointestinal cancer, breast cancer and lung cancer.

To date, research on fermented ginseng using ginseng and mushroom spawn has been conducted. In particular, Korean Patent Publication No. 2008-20749 discloses ginseng and its extract fermented with Cordyceps sinensis and its use, Korean Patent Publication No. 2004-108476 In the manufacturing method of functional bio-converted ginseng using edible and medicinal mushroom culture, Korean Patent Publication No. 2006-62083 is known how to convert ginseng saponin composition.

However, as described above, a method for preparing fermented ginseng using the spawn of Sichuan mushroom and Cordyceps sinensis is known, but research and development of fermented ginseng using spawn of chaga, ganoderma lucidum and fingerling mushrooms have similar effects. It is not true.

The present inventors have tested the anticancer activity of fermented ginseng using endless mushroom spawn, chaga, ganoderma lucidum, ganoderma lucidum, fingerling mushrooms, chaga, ganoderma lucidum, fingerling mushrooms Fermenting the spawn together with ginseng confirmed that the anticancer effect of the mushroom fermented ginseng was markedly increased by the biological conversion ability of the mushroom spawn, thus completing the present invention.

Therefore, the problem to be solved by the present invention is fermentation of ginseng and mushroom spawn can easily ingest the active ingredient of ginseng irrespective of the constitution of human, mushroom fermentation can maximize the anti-cancer effect of ginseng and mushroom spawn To provide ginseng powder.

Furthermore, an object of the present invention is to provide a method for producing a health food for cancer containing the mushroom fermented ginseng powder.

Anti-cancer health food according to the present invention for solving the above problems includes fermented ginseng powder fermented with mushroom spawn.

Method for producing a health food for cancer according to the present invention for solving the other problems is the step of drying ginseng; Sterilizing the ginseng; Inoculating the ginseng and mushroom spawn; Fermenting the mushroom spawn and ginseng to make a fermented product; Pulverizing the fermented product.

The mushroom spawn may be at least one selected from the group consisting of chaga ( Inonotus obliquus ), Ganoderma lucidum ( Ganoderma lucidum ), fingerlings ( Coriorus versicolor ) spawn.

The ginseng may be at least one selected from the group consisting of ginseng, white ginseng, direct ginseng, half-goak ginseng, grain ginseng, blood white ginseng, red ginseng, cheonsam, jisam, sheep ginseng, jeolsam, taegeuk ginseng, misam ginseng, sumi ginseng.

The step of drying the ginseng uses a conventional ginseng drying method, in the present invention is dried for 10 to 36 hours at 15 ~ 30 ℃ to dry the moisture content of 60 to 80%.

Sterilizing the ginseng using a conventional ginseng sterilization method, in the present invention uses an autoclave.

Inoculating the ginseng and mushroom spawn is inoculated in an amount of 1 to 30% of the ginseng used weight, preferably inoculated in an amount of 5 to 20%.

Fermenting the mushroom spawn and ginseng to make the fermentation is a solid fermentation at 20 ~ 30 ℃ for 30 to 60 days.

The step of pulverizing the pulverized product is dried by hot air at 40 ~ 60 ℃ after the fermentation, and then pulverized by a conventional method of grinding ginseng to prepare a mushroom fermented ginseng powder.

When the mushroom fermented ginseng extract of the present invention is used as a food, the extract may be added as it is or used with other foods or food ingredients, and may be appropriately used according to a conventional method. The mixed amount of the active ingredient may be appropriately determined according to its purpose of use (prevention, health or therapeutic treatment), and may be used according to the effective dose of the pharmaceutical composition, but for health and hygiene purposes or to control health. In the case of long-term intake of the target may be below the above range, the active ingredient may be used in an amount above the above range because there is no problem in terms of safety.

In addition, the present invention provides a health functional food for preventing or improving cancer comprising the extract of the present invention and a food supplement acceptable food supplement. The health functional food of the present invention includes the form of tablets, capsules, pills or liquids, and the food to which the compound of the present invention can be added, for example, various foods, beverages, gums, teas, vitamin complexes, etc. And health functional foods. Specifically, it provides a health functional food for cancer prevention or improvement comprising an extract containing the extract of the present invention as an active ingredient and a food supplement acceptable food supplement additive.

Hereinafter, the present invention will be described in detail by way of examples.

However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

The anti-cancer health food containing the mushroom fermented ginseng powder according to the present invention increases the absorption power in vivo through metabolic process by fermenting mushroom spawn in ginseng and making it in the form of ginseng saponin which can be absorbed in vivo. Regardless of the presence of enteric microorganisms, anyone can standardize the effects of ginseng saponin.

In particular, through the fermentation of chaga, ganoderma lucidum, fingerling and ginseng, the production of various types of ginsenosides than ginseng or sterile ginseng was confirmed by the action of mushroom strains, and the content of ginseng components was increased. Increases Rg1, which inhibits platelet aggregation, Re, which has anticancer effect, Rb2, which activates macrophages, and Rd, which expands vasodilation, and Ganoderma lucidum fermented ginseng increases Rd, which causes vasodilation, and fingering Fermented ginseng mushrooms have increased anti-cancer and pharmacological effects than conventional ginseng or sterile ginseng by increasing Rc, which promotes protein and lipid synthesis, and analgesic Rc, and Rd, which stimulates the secretion of adrenal stimulus hormone.

Therefore, fermented ginseng increases the main ingredient of ginseng and saponin, the main ingredient of anti-cancer activity of ginseng, so it shows higher effect than the anti-cancer effect of mushrooms and ginseng.

Fermented ginseng prepared according to the manufacturing method of the present invention can be applied to fermented foods or functional foods, etc. to increase shelf life and improve the medical functionality of the fermented foods or functional foods.

Hereinafter, preferred embodiments of the present invention will be described. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms.

Example 1 Ginseng Powder

After washing fresh ginseng with clean water, drying it at 25 ℃ for 24 hours to adjust the water content to 70%, cut the fresh ginseng into 1cm size using a sterilized knife or scissors, and put 200g each in a plastic pack. After sealing with), sterilizer 121 ℃, sterilized for 10 minutes at 1.2kgf / cm ² conditions, the plastic pack was taken out of the sterilizer, allowed to cool, and then hot-air dried at 50 ℃, and then ground to prepare a ginseng powder.

Example 2 Chaga Mushroom Powder

Chaga mushroom powder was prepared in the same manner as in Example 1, using chaga mushroom.

Example 3 Ganoderma lucidum powder

In the same manner as in Example 1, using a ganoderma lucidum mushroom ganoderma lucidum powder was prepared.

Example 4 Cloudy Mushroom Powder

Cloudy mushroom powder was prepared in the same manner as in Example 1 except for using fingering mushrooms.

Example 5 Chaga Mushroom Fermented Ginseng Powder

After cooling the sterile ginseng sterilized in the process in Example 1 10% inoculated chaga mushroom spawn, solid fermentation at 28 ℃ 50 days, harvested and hot-air dried at 50 ℃, and then pulverized to prepare a chaga mushroom fermented ginseng powder.

Example 6 Fermented Ginseng Powder

In the same manner as in Example 5, inoculated Ganoderma lucidum seedling to prepare a Ganoderma lucidum fermented ginseng powder.

Example 7 Fermented Ginseng Powder for Cloudy Mushroom

In the same manner as in Example 5, by inoculating the fingertips of fingering mushrooms to prepare fermented ginseng powder of fingering mushrooms.

Comparative Example 1 Taxol

Taxol was purchased from SIGMA (PO115) as a positive control.

Experimental Example 1 Anticancer Effect Test of Prepared Ginseng, Mushroom, and Fermented Ginseng Powder

Ginseng powder, chaga mushroom powder, ganoderma lucidum powder, fingering mushroom powder, chaga mushroom fermented ginseng powder, ganoderma lucidum fermented ginseng powder, fingerling mushroom fermented ginseng powder prepared in Examples 1-7 and Taxol (Taxol) of Comparative Example 1 Animal experiments were conducted to verify the anticancer effects.

The rats used in the experiment were Japanese black rats (BDF ₁ male) purchased from Central Experimental Animal Co., Ltd., and their weight was 20 ± 2g, the breeding temperature was 22 ± 2 ℃, and the solid feed (Samyang Feed (Co.) and water) The first solvent (EP) was used as the extraction solvent, and the sample was used as a sterile saline solution prepared by Korea Pharmaceutical Co., Ltd. RPMI 1640 medium used for cancer cell culture was used as a medium provided by WelGENE Inc. For Trypsin-EDTA, GIBCO Co., Ltd. was used as a test tube for cell culture, a flask for culture, and a filter for obtaining cancer cells, Falcon Co., Ltd. The devices used for cancer transplantation were sterilized at 120 ° C. for 30 minutes. The volume of the cancer was measured using a digital caliper (Digital Caliper).

The lung cancer (Lewis Lung Carcinoma: LLC) cells used in the experiment were used by the Korea Research Institute of Bioscience and Biotechnology, and the lung cancer (Lewis Lung Carcinoma: LLC) cells were cultured in an incubator at 37 ° C with RPMI 1640 medium containing 10% FBS. Trypsin-EDTA was used to separate from the attachment surface. When cells were filled in a 200 ml culture flask, two animals were transplanted and grown in vivo, and then animal experiments were performed using them.

Anti-cancer animal experiment is about 15 × 15 × 15mm ³ Cancer cells were isolated from other cells using a Falcon filter after surgery on tumors grown to size, and the cancer cells obtained by washing several times were injected into the axilla of experimental animals by concentration of 2 × 10 ⁵ cells / ml. Induced. For 15 days after transplantation, ginseng powder, chaga mushroom powder, ganoderma lucidum powder, fingerling mushroom powder, chaga mushroom fermented ginseng powder, ganoderma lucidum ginseng powder, and fingering fermented ginseng powder were dosed to Japanese black rats at a dose of 100mg / kg, respectively. Orally, 25 mg / kg of Taxol (purchased from Sigma) was intraperitoneally injected once daily for 14 days as a positive control. The administration of fermented ginseng powder was stopped 15 days after cancer transplantation.

Test Example 1 Comparative Evaluation of Anticancer Effects of Prepared Mushroom Fermented Ginseng Powder

Comparative evaluation of the anticancer effect of Examples 1 to 7 and Comparative Example 1 prepared by the present invention was evaluated through an anticancer animal experiment, and after stopping the administration of fermented ginseng powder and Taxol 15 days after cancer transplantation, the digital caliper The results were measured using (Digital Caliper). Calculation of the volume of the cancer and cancer volume inhibition rate is the volume of the cancer (Tumor Volume, TV) = Length (mm) × Width 2/2. Inhibition Ratio of Tumor Volume, IRTV) = (TV of control-TV of test) x 100 / TV of control. After measuring the volume of the cancer, the weight of the cancer was obtained by measuring the mass of the cancer, and the inhibition rate was the inhibition ratio of Tumor Weight (IRTW) = (TW of control-TW of test) x 100 / TW of control. Calculated by the formula.

All data were expressed as mean ± SD. The analysis was carried out by Student's t- test, and p values were significant when 0.05 or less. The experimental results are shown in Table 1 below.

      Table 1. Comparative Evaluation of Anticancer Effects of Prepared Mushroom Fermented Ginseng Powder

division Volume
(mg / kg) Tumor size
(mm ³ ) Cancer volume suppression rate
(%) Tumor weight
(g) Dark weight suppression rate
(%)
Control
0
1885 ± 235
0
1.67 ± 0.3
0
Ginseng powder
100
1551 ± 126
17.7
1.39 ± 0.3
16.4
Chaga Powder
100
1592 ± 125
15.5
1.43 ± 0.2
14.1
Ganoderma lucidum powder
100
1688 ± 101
10.4
1.50 ± 0.1
9.8
Fingering mushroom powder
100
1721 ± 135
8.7
1.53 ± 0.4
8.1 Chaga Mushroom
Fermented Ginseng Powder
100
1251 ± 133
33.6
1.12 ± 121
33.0 Ganoderma lucidum mushroom
Fermented Ginseng Powder
100
1308 ± 98
30.6
1.16 ± 137
29.8 Fingering mushroom
Fermented Ginseng Powder
100
1417 ± 145
24.8
1.27 ± 111
24.1 Taxol
(Positive control)
25
1206 ± 126
34.0
1.06 ± 103
36.3

       Table 1 shows the results of calculating the inhibition rate by stopping the sample administration and measuring the volume of the cancer and the weight of the cancer 15 days after transplantation of lung cancer (LLC) cells into the mouse. The ginseng powder (Example 1) showed a cancer volume inhibition rate of 17.7% and a cancer weight inhibition rate of 16.4%, and the cancer volume inhibition rate of Chaga mushroom powder (Example 2) was 15.5%, and the cancer weight inhibition rate was 14.1%, the cancer volume inhibition rate of Ganoderma lucidum powder (Example 3) was 10.4%, the cancer weight inhibition rate was 9.8%, the cancer volume inhibition rate of the fingering mushroom powder (Example 4) was 8.7%, cancer The weight gain ratio was 8.1%, showing a low anticancer effect. However, Chaga mushroom fermented ginseng powder (Example 5), which fermented mushroom spawn on ginseng, showed the highest anticancer effect of 33.6% of cancer volume inhibition rate and 33.0% of cancer weight inhibition rate. 6) and the fermented ginseng powder of ginseng mushroom (Example 7) showed good anticancer effect with inhibition rate of 30.6% and 24.8%, and cancer weight inhibition rate of 29.8% and 24.1%. Taxol showed anticancer effect of 34.0% for cancer volume inhibition and 36.3% for cancer weight inhibition.

       Therefore, chaga and ginseng fermented ginseng, Ganoderma lucidum and Ginseng fermented ginseng, which have fermented ginseng together with mushroom spawn, have better anticancer effects than conventional ginseng and mushrooms and show anticancer effects similar to Taxol. It has been confirmed that the effect is sufficient as a chemical cancer preventive agent or cancer improvement for ingestion to prevent cancer.

Experimental Example 2 Analysis of Saponin Content of Fermented Ginseng Using HPLC

Saponin content of ginseng powder, chaga mushroom fermented ginseng powder, Ganoderma lucidum fermented ginseng powder, and fingering mushroom fermented ginseng powder according to the present invention were analyzed by HPLC.

HPLC analysis conditions were used as mobile phase, A pump was H ₂ O and B pump was AcCN, and the time difference was used. The oven temperature of the column was 30 ℃ and UV detection was 230nm. Is Discovery C18 (4.6 × 250 mm, 5 μm, Supelco Co.).

As a result, the Chaga mushroom fermented ginseng powder of Example 5 according to the present invention (see Fig. 4) was increased Rg1, Re, Rb2, Rd than the ginseng powder of Example 1 (see FIG. 2), and the ganoderma lucidum of Example 6 The mushroom fermented ginseng powder (see FIG. 5) increased Rd, and the fingering mushroom fermented ginseng powder of Example 7 (see FIG. 6) was found to increase Rc and Rd.

As a result, chaga fermented ginseng powder, Ganoderma lucidum fermented ginseng powder, and fingerlings fermented ginseng powder, which had fermented ginseng together with mushroom spawn, had better saponin content than ginseng powder not fermented with mushroom spawn.

Use Example 1: Preparation of Anticancer Functional Tablets

70 g of lactose, 75 g of potato starch, and 10 g of colloidal silicic acid were mixed into 100 g of the mushroom fermented ginseng powder of Examples 5 to 7. 10% gelatin solution was added to the mixture, pulverized, filtered through 14 meshes, dried, and then 65 g of potato starch, 25 g of talc and 2 g of magnesium stearate were added, and the resulting mixture was mixed to obtain 400 mg of tablets.

<Use Example 2: Preparation of anticancer functional capsule formulation>

60% by weight of mushroom fermented ginseng powder, 5% by weight of jujube powder and 25% by weight of crystalline cellulose were mixed with 70% alcohol, dried at 60 ° C. for 30 hours, then pulverized and filtered through 50 mesh. It was prepared in 500mg / cap unit by filling in a capsule.

Use Example 3: Preparation of Anticancer Functional Drink

0.48-1.28 mg of mushroom fermented ginseng powder of Examples 5-7, 522 mg of honey, chitosanamide 5 mg, nicotinic acid amide 10 mg, riboflavin sodium 3 mg, pyridoxine hydrochloride 2 mg, inositol 30 mg, orthoic acid 50 mg And 200 ml of water in a composition and content to prepare a beverage using conventional methods.

1 is a flow chart sequentially showing the manufacturing process of mushroom fermented ginseng powder according to the present invention.

Figure 2 is a chromatograph analyzed by HPLC the content of saponin standard compared to the saponin content of the fermented ginseng powder according to the present invention.

3 is a chromatograph analyzed by HPLC of the saponin content of the ginseng powder according to the present invention.

Figure 4 is a chromatograph analyzed by HPLC the saponin content of chaga mushroom fermented ginseng powder according to the present invention.

5 is a chromatograph analyzed by HPLC of the saponin content of Ganoderma lucidum fermented ginseng powder according to the present invention.

6 is a chromatograph obtained by analyzing the saponin content of the fingertips ginseng fermented ginseng powder according to the present invention.

Claims (6)

In the production of mushroom fermented ginseng powder, Drying ginseng to 60 to 80% of water content (first step); Sterilizing the ginseng (second step); One or more spawns of Ganoderma lucidum and Coriorus versioclor are inoculated into the ginseng in an amount of 5-20% of the ginseng weight, and solid fermentation at 20-30 ° C for 30-60 days Making step (third step); And Pulverizing the fermented product (fourth process); Method of producing a mushroom fermented ginseng powder comprising a. The method of claim 1, The ginseng is a mushroom fermented ginseng, characterized in that at least one selected from the group comprising ginseng, white ginseng, direct ginseng, half-goak ginseng, Goksam, blood white ginseng, red ginseng, Cheonsam, Jisam, Yangsam, Jeolsam, Taegeuksam, Misam, Sumisam Method of making the powder. delete delete A health food for cancer prevention or improvement comprising the mushroom fermented ginseng powder prepared by the method of claim 1 or 2. The method of claim 5, The food is a cancer prevention or improvement health food, characterized in that the form of tablets, capsules, or beverages.

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