KR20100008220A - Composition for preventing and treating colon cancer containing extract of mushroom and herb medicine - Google Patents

Abstract

PURPOSE: A composition containing Inonotus obliqua extract and herb extract is provided to treat and prevent colon cancer without side effects. CONSTITUTION: A composition for preventing or treating colon cancer contains Inonotus obliqua extract as an active ingredient. The composition further contains Glycyrrhiza uralensis extract or red ginseng extract as an active ingredient. The Inonotus obliqua extract is ethanol extract. The composition contains 0.00001-99.9 weight% of mixture extract containing Inonotus obliqua extract, Glycyrrhiza uralensis extract, and red ginseng extract. The red ginseng extract is fermented red ginseng extract.

Description

Composition for preventing and treating colon cancer containing extract of mushroom and herb medicine}

The present invention relates to a composition for preventing or treating colorectal cancer containing mushroom and herbal extract. In particular, the present invention relates to a composition for preventing or treating colon cancer containing chaga extract. More specifically, the present invention relates to a composition for preventing or treating colon cancer, comprising a mixture of chaga extract, licorice extract, and red ginseng extract as an active ingredient.

Chaga is a perennial basidiomycete belonging to the genus Hyenochaetaceae, distributed in the cold and humid northern hemisphere above 45 degrees north latitude, and grows in black to form a black nucleus nucleus that is parasitic on birch. It is engrossed by the virus, eats sap, and grows, usually for 15 to 20 years. It is also found in alder, willow and maple trees, but these are known to have low efficacy. Among 15 years or more, only Grade 1 dried at a thickness of 10cm or more, moisture content of 14% or less and 60 ℃ or less is used for medicinal purposes, and the rest is used for brewing or making tea. Chaga's ingredients include melanin, flavonoids, triterpenes, obliquol, lannosterol, inositol, agaric acid, polyphenols, β-heteroglucans, lignin and alkaloids. The furnace contains Ca, Mg, Fe and Mn, and is used in the civilian to control blood pressure, enhance physical resistance, and to treat many diseases such as diabetes, neuralgia, and nervous breakdown.

Studies on the physiological activity of chaga mushrooms include antimutagenicity (Saitoh et al., Dosa 46 46 (1996)), antiviral (Kahlos K et al., Fitoterapia 57: 4 (1996); and Ichmura T et al., Biosci. Biotechnol. Biochem. 62: 575 (1998), and antioxidant (Cui Y et al., J. Ethnopharmacol. 96 (1-2): 79 (2005); and Babitskai, VG et al. , Prikladmaya Bioxhimiya Mikrobiologiya, 36: 439 (2000)) and antidiabetic (Mizuno T et al., Int. J. Med. Mushroom 1: 301 (1999)).

Licorice is a perennial herb of the Rosacea legumes, used as a sweetener for sweet roots, and has been used traditionally in Chinese medicine as a medicine for lung and inflammatory diseases in Tibet and India (Demizu S et al. Chem Pharm Bull 36: 3474-3479, 1988). However, no clear mechanism of licorice's inflammatory effects has been identified. In addition, licorice extract has been added to enhance this effect or to make the medicinal herbs less bitter (Vaya J et al., Free Radic Biol Med 23: 302-313, 1997).

Licorice (Glycyrrhizae radix) in oriental pharmacology is a generally safe herbal medicine frequently applied in many combination prescriptions (Kamei, J., et al., Eur. J. Pharmacol., 469, pp 159-163, 2003). Root stock is columnar, main root is very long, coarse and large, and the outer shell is reddish brown to dark brown. Root is sweet and is used as sweetener and herbal medicine. It is distributed in northeastern China, Siberia and Mongolia. The roots and roots of licorice contain triterpene system saponins and glycyrrhizin, which are 2-glucuron of glycyrrhizin acid. Acid glycoside, a sweet component of licorice. This glycoside has no blood but glycyrrhetin acid. Among the hydrolyzed components of licorice, uralene acid was extracted and it was proved that it was 18α-glycirrhetin acid (Information et al., Ph.D.

Red ginseng (Ginseng radix) is a dry root of Panax ginseng C. A. MEYER belonging to the Araliaceae. Red ginseng is steamed and dried ginseng, taste is sweet and slightly bitter, flat nature, and enters the nasal and lung (대표적인) and acts as a typical medicine in the main medicine is Daebowon period, delivery date Pyeongcheon, Saengjinji There is a sedation and excitement effect on the central nervous system, and the circulatory system prevents hypertension or atherosclerosis.

The main ingredients are saponin (ginsenosides), panaxynol, beta-elemene and maltol. Aqueous extract of red ginseng is used to treat anemia, diabetes, insomnia, gastritis, blood pressure abnormalities, dyspepsia, overstrain, and fatigue It has been studied to contain a variety of tryterpene glycosides called saponin (ginsenosides) (Baranov AI, J. Ethnopharmacol., 6, 339-53, 1982; Chong SK, et al., Postgrad Med. J., 64, 841-6, 1988).

In addition, red ginseng has been reported to have various pharmacological effects including antihypertensive, cardiotonic, sedative, aphrodisiac, anti-aging and antioxidant activities (Gillis CN, Biochem. Pharmacol). , 54, 1-8, 1997).

As a method of treating colorectal cancer, surgical methods are widely used. In particular, among colon cancer patients of locally advanced or metastatic disease, the prognosis is very disadvantageous due to high incidence and mortality.

On the other hand, as a method for treating colorectal cancer, drug therapies that have less favorable results than surgery have been tried. The anti-tumor compound 5-fluorouracil (5-FU) is the main drug of choice in the treatment of colorectal cancer, and its use has only proven to be marginally effective. 5-FU may temporarily reduce colorectal cancer size, but little evidence indicates that the patient's survival is substantially extended or "healed" (based on a 5-year reduction). Although chemotherapy with 5-FU is used in patients with disease metastasis to the liver, only a temporary improvement is observed in only 25% or less of such cases, and overall survival is not significantly affected [Lamont, J. LaMont, JT and Easelbacher, K., Harrison's Principles of Internal Medicine (10th edition), McGraw-Hill, New York, p. 1764 (1983). Despite the marginal effects of 5-FU, there were no other drugs or combination treatments that apparently appeared to be more effective [Su Baker, P.H. Back, homology; Woolley, P. V., et al., New Eng. J. Med., 312: 1465 (1985).

Given the very poor 5-year survival rate (about 50% or less) in patients undergoing conventional treatment for colorectal cancer, such as surgical resection or chemotherapy using 5-FU, It would then be very useful to find new ways to effectively treat malignant colorectal cancer in humans using drugs or compounds, such as to help confirm the diagnosis or to remove the volume of cancer. Since surgery cannot remove all of the cancerous tissues, drug treatment will also be very useful for patients with advanced disease that have spread or spread to various organs.

One embodiment of the present invention is to provide a composition for effectively preventing and treating colorectal cancer.

One embodiment of the present invention aims to maximize the prevention and treatment effect of colorectal cancer by combining several natural extracts.

One embodiment of the present invention is to provide a preventive and therapeutic agent for colorectal cancer with a significantly low side effects to the human body by using a natural extract.

One embodiment of the present invention is characterized in that it comprises chaga extract as an active ingredient.

One embodiment of the present invention is characterized in that it comprises chaga mushroom extract, licorice extract and red ginseng extract as an active ingredient.

Using the present invention, it is possible to obtain an effect that can effectively prevent or treat colorectal cancer without side effects or toxicity to the human body.

Hereinafter, the present invention will be described in more detail.

The scientific name of chaga is Inonotus obliqua or Fuscoporia obliqua (persoon ex Fires). In Russia it is called Chaga, and in Japan it is called Kabanoanatake. Mycobacterium forms a black surface, with many cracks on both sides, and yellowish brown inside, similar to Phellinus linteus. Mycelium, a hard mass made by densely packed mycelia, is mainly used for medicinal purposes. The diameter of the bacterium is large, about 10-20 cm. The fruiting body has a thickness of about 2 to 8mm, a hole on the front, and several bristle bodies.

Chaga occurs in birch (Betula sp.), Especially birch (Betula platyphylla var. Japonica). It is a semi-reflective parasitic mushroom that is attached to a birch tree like a black rock mass, and the birch tree gradually dies as the mass grows larger.

Chaga was originally used for natural harvesting, but it is preferable to artificially cultivate because the quantity is small and requires a lot of effort to collect. There are various methods of culturing, but among them, sawdust culturing and liquid culturing are preferred. In addition, it is also possible to use a mycelium obtained by growing the mycelium or artificially grown mycelia of chaga mycelia on artificial green trees of birch. Sawdust cultivation is prepared by first preparing the sawdust of birch trees, lime, rice, mixed with water, and then adjusting the moisture, and heat sterilization of the mixture into a heat-resistant container or bag to obtain a sawdust incubator. The sawdust incubator is cooled to a growth temperature of chaga mycelium, preferably 20 ° C. or lower, and then the mycelium is grown. When mycelia grow white in the incubator for several months, it is collected and processed as needed to extract the active ingredient, which is used as an active ingredient.

There are several ways to extract the active ingredient of chaga. Especially, the mycelia of natural chaga or cultured chaga can be treated with PBS solution, butanol, ethyl alcohol, ethyl acetate or acetone, and the active ingredient can be taken out from each insoluble matter. Alternatively, the mycelium component or mycelium extract of natural chaga or cultured chaga may be adsorbed with carbon or charcoal, and the active ingredient may be taken out from each of the nonadsorbed substances. The active ingredient can also be obtained by hot water extraction in which natural chaga or cultured chaga are boiled with water at various pHs (eg, boiled for 60 minutes). These extract active ingredients are recognized to be water-soluble, heat-resistant and acid-resistant stable substances. This extract component may be taken orally as it is, or may be mixed with medicines or foodstuffs and consumed thereof.

In one embodiment of the present invention, the crude licorice extract is water, water of 1 to 4 carbon atoms or anhydrous lower alcohol, mixed solvent of the lower alcohol and water, or acetone, ethyl acetate, chloroform, 1,3-butylene It can be obtained by using an extraction solvent such as glycol, butyl acetate and the like. Preferably, the licorice extract is obtained using a hydrous lower alcohol, most preferably ethanol.

For example, licorice is first dried and then ground and powdered, and then about 1 to 10 times the dry weight, preferably about 2 to 6 times water or lower alcohol, and about 20 to 60 ° C., preferably about 30 to It can be obtained by ultrasonic extraction, cooling and filtration for about 1 to 5 hours, preferably about 1.5 to 3 hours while heating to 70 ℃, and concentrated under reduced pressure using a rotary evaporator. At this time, the filtration process may be repeated two or more times with respect to the residue remaining after filtration.

In one embodiment of the present invention, the crude red ginseng extract is water, water of 1 to 4 carbon atoms or anhydrous lower alcohol, a mixed solvent of the lower alcohol and water, or acetone, ethyl acetate, chloroform, 1,3-butylene It can be obtained by using an extraction solvent such as glycol, butyl acetate and the like. Preferably, the red ginseng extract is obtained using a hydrous lower alcohol, most preferably ethanol.

For example, the red ginseng extract according to an embodiment of the present invention may be prepared as follows: Red ginseng prepared by the conventional red ginseng manufacturing method by steaming ginseng is washed with water and cooled for 1 to 48 hours, preferably 10 hours. The red ginseng chilled for a while is put in a polar solvent such as 1 to 15 times water, ethanol or methanol, preferably 5 to 10 times water, heated at 40 to 100 ° C., preferably 70 to 90 ° C., and then heated. The extract was repeatedly extracted 1 to 10 times, filtered under reduced pressure, and the filtered extract was mixed and concentrated under reduced pressure at 20 to 100 ° C., preferably at 50 to 70 ° C., using a rotary vacuum concentrator to remove water and carbon atoms. Crude extract which is a soluble extract according to the lower alcohol of 4 or a mixed solvent thereof can be obtained.

On the other hand, it will be apparent to those skilled in the art that the extract of the present invention can be obtained not only the above-described extraction solvent but also other licorice extracts having substantially the same effect.

In addition, the extract of the present invention includes not only the extract by the above-mentioned extraction solvent, but also the extract that has undergone a conventional purification process. Activity obtained through various purification methods additionally performed, for example, separation using ultrafiltration membranes having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). The fraction is also included in the licorice extract of the present invention.

Extracts of the present invention may be prepared in powder form by additional processes such as distillation under reduced pressure and freeze drying or spray drying.

In one embodiment of the present invention, the red ginseng extract may be a fermented red ginseng extract. By fermenting red ginseng, the active ingredients of red ginseng are easily absorbed into the human body, and the ingredients present in red ginseng in trace amounts can be strengthened. For fermentation of red ginseng, a method of fermenting red ginseng using intestinal microorganisms or treating enzymes is used. For example, Korean Patent Laid-Open Publication No. 2003-61756 discloses a method for producing a ginseng fermentation broth having excellent functionality and functionality, which is characterized by fermenting ginseng into aspergillus and then starch and protease. In one embodiment of the present invention, fermented red ginseng prepared by the method as described above can be used.

When the composition according to an embodiment of the present invention includes a combination of chaga mushroom extract, licorice extract and red ginseng extract as an active ingredient, the weight ratio of each extract may be 1 to 5: 1 to 10: 0.5 to 3. When belonging to this range can be maximized synergistic effect between each extract.

The composition according to the present invention may contain the chaga mushroom extract, or a combination of chaga mushroom extract, licorice extract and red ginseng extract in an amount of 0.0001 to 99.9% by weight based on the total weight of the composition. 0.1-60.0% by weight is more preferable in view of effective effects and stability and formulation stability.

The composition according to the invention may be a pharmaceutical composition. In the case of pharmaceutical compositions, it may be administered orally, parenterally, rectally, topically, transdermally, intravenously, intramuscularly, intraperitoneally, subcutaneously, etc., with the most preferred route of administration being oral administration.

In addition, the dosage of the active ingredient will vary depending on the age, sex and weight of the subject to be treated, the specific disease or pathology to be treated, the severity of the disease or pathology, the route of administration and the judgment of the prescriber. Dosage determination based on these factors is within the level of skill in the art. Generally dosages range from 0.0001 mg / kg / day to approximately 2000 g / kg / day.

On the other hand, depending on the intended dosage form, the pharmaceutical composition may be in solid, semisolid or liquid dosage form. Examples of dosage forms include, but are not limited to, tablets, capsules, chewing tablets, small bags, granules, powders, liquid solutions, suspensions, dispersions, emulsions, syrups, suppositories, and the like. The active ingredient may be encapsulated in liposomes, microparticles or microcapsules.

On the other hand, in the case of formulating the extract of the active ingredient of the present invention in the form of tablets, capsules, chewing tablets, small bags, granules, powders, liquid solutions, suspensions, dispersions, emulsions, syrups for the purpose of oral administration, Gum arabic, corn starch, binders such as microcrystalline cellulose or gelatin, excipients such as dicalcium phosphate or lactose, disintegrants such as alginic acid, corn starch or potato starch, lubricants such as magnesium stearate, sucrose or saccharin and Such sweeteners and flavoring agents such as peppermint, methyl salicylate or fruit flavors may be included, and when the dosage unit form is a capsule, liquid carriers such as polyethylene glycol or fatty oils may be included in addition to the above components.

In addition, the food composition according to an embodiment of the present invention provides a health functional food composition to which the food supplement is added to the food acceptable food supplement. For example, there are various foods such as chewing gum, caramel products, candy, ice cream and confectionery, beverage products such as soft drinks, mineral water and alcoholic beverages, and health functional foods including vitamins and minerals.

At this time, the amount of the extract in the food may be added to 0.001 to 99.9% by weight of the total food weight, in the beverage may be added in a ratio of 0.001 to 0.1 g, more preferably 0.05 to 0.1 g based on 100 ml.

The beverage containing the extract of the present invention is not particularly limited to other ingredients except for containing the extract as an essential ingredient in the indicated ratio, and may contain various flavors or natural carbohydrates as additional ingredients, such as ordinary drinks. .

In addition to the above, the functional food composition of the present invention includes various nutrients, vitamins, minerals (electrolytes), synthetic flavors such as synthetic and natural flavors, colorants and enhancers (such as cheese and chocolate), pectic acid and salts thereof, alginic acid And salts thereof, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. In addition, the health functional food compositions of the present invention may contain fruit flesh for the production of natural fruit juices and fruit juice drinks and vegetable drinks. These components can be used independently or in combination. The proportion of such additives is not so critical but is generally selected from the range of 0 to about 20 parts by weight per 100 parts by weight of the composition of the present invention.

In one embodiment of the present invention, the composition for preventing or treating colorectal cancer may be administered in parallel with a surgical method for treating colorectal cancer. The composition can be administered before or after surgery. In addition, in one embodiment of the present invention, the composition may be administered in combination with other drugs.

Hereinafter, the present invention will be described in more detail with reference to Examples, but it is obvious that the scope of the present invention is not limited by the following Examples.

Example

Example 1 Preparation of Extract

1-1. Preparation of Chaga Extract

200 g of crushed chaga was put in 1000m of ethanol and stirred for 30 minutes. Thereafter, the solvent was removed to obtain a chaga extract. The extract thus obtained was lyophilized.

1-2. Preparation of Licorice Ethanol Extract

After drying, 15 L of 100% methanol was added to 4 kg of crushed licorice, sonicated for 2 hours while heating to 37 ° C, cooled and filtered, and concentrated under reduced pressure using a rotary evaporator to obtain 750 g of ethanol extract of licorice. .

1-3. Preparation of Red Ginseng Extract

Fermented red ginseng powder (Kim Jung Moon Co., Ltd., Aloe, Korea) was purchased and used.

Experimental Example 1 MTT Analysis

Situation mushroom extract, chaga mushroom extract (Example 1), licorice extract (Example 1), fermented red ginseng powder (Example 1), Ganoderma lucidum extract, white sperm extract with the control, 100ug / ml, 50ug / ml , 25ug / ml, 12.5ug / ml, 6.25ug / ml concentrations were treated in HT-29 Colon cancer cells for 48 hours and then cell viability was confirmed. The results are shown in FIG.

Experimental Example 2 Experiments on the Effect of Licorice Extract on the Formation of Colorectal Cancer Cell Spheres

Chaga mushroom extract prepared in Example 1 was added to 100ml of distilled water at a concentration of 33.33mg / ml and stirred. The solution thus prepared was added to HT-29 Colon cancer cells at concentrations of 25 ug / ml, 50 ug / ml and 100 ug / ml. Thereafter, HT-29 cells were seeded for 3 weeks in a condition where they could not adhere to the bottom, and the spheres were counted. The result is as shown in FIG.

As shown in Figure 2, it can be seen that the number of spheres per HT-29 cells decreases in proportion to the concentration of chaga extract. That is, chaga extract was confirmed to effectively inhibit the spear formation of HT-29 cells.

Formulation example

Hereinafter, the formulation examples of the composition will be described, but the present invention is not intended to be limited thereto but merely to be described in detail.

Formulation example  1. Preparation of Tablets

Chaga extract of Example 1 ...... 100 mg

Corn starch ............... 68 mg

Lactose ......... 90 mg

Microcrystalline Cellulose ............. 40 mg

Magnesium Stearate ..... 2 mg

According to the conventional method of preparing tablets, the above ingredients were added to the indicated contents, mixed uniformly, stirred and granulated. After drying, using a tableting machine, a desired tablet containing 100 mg of chaga extract, which is one active ingredient, was prepared.

Formulation example  2. Preparation of capsule

Chaga extract of Example 1 .. 60 mg

Red ginseng extract of Example 1 ............ 40mg

Corn starch ..... 68 mg

Lactose ......... 90 mg

Microcrystalline Cellulose .............. 40 mg

Magnesium Stearate ......... 2 mg

According to the conventional method for preparing the capsules, the above-mentioned ingredients were added and mixed uniformly, and then the desired capsules were prepared by filling into gelatin capsules of an appropriate size to include 100 mg of extract per capsule.

Formulation example  3. Chewing Definition  Produce

Chaga Extract of Example 1 ....... 30 mg

Licorice extract of Example 1 ... 60 mg

Red Ginseng Extract of Example 1 ...................................... 10mg

Syrup to granule ......... 240 mg

Fragrance ......................................... 2 mg

Corn starch ..... 60 mg

Mannitol ......................................... 100 mg

Magnesium Stearate ............... 20 mg

Purified water ............................

According to the conventional method of preparing chewing tablets, the above ingredients were mixed in an appropriate amount of water, dissolved while heating, cooled and placed in a molding machine to prepare chewing tablets of a desired shape, containing 100 mg of extract per one. .

Formulation example  4. Manufacture of Candy

Chaga extract of Example 1 ....... 100 mg

Syrup to granule ......... 640 mg

Fragrance ......................................... 2 mg

Starch syrup ............... 230 mg

50% tartaric acid ................................. 20 mg

Purified water ...............

The granulated sugar syrup was heated to 110 ° C. while completely dissolving in a small amount of water, and then the remaining water and starch syrup dissolved in the reference extract were added to raise the temperature to 145 ° C. The fire was turned off, the fragrance and tartaric acid were added, mixed, cooled to 75-80 ° C., and then molded with a forming roller to prepare a candy containing extract.

Formulation example  5. Manufacture of drinks

Chaga extract of Example 1 ............ 100 mg

Concentrated Juice ........... 2 g

Sucrose ......................................... 12 g

Sodium Citrate ... 100 mg

Fragrance ............... 70 mg

Water ............................................

According to a conventional method for preparing a beverage, the above ingredients are mixed in an appropriate amount of water to a given content, heated to dissolve, cooled, and filled into a container, which contains 100 mg of chaga extract per bottle of 200 ml of beverage. A beverage was prepared.

Figure 1 shows the results of analysis by MTT ASSAY after processing chaga extract, licorice extract and red ginseng extract to HT-29 cells.

Figure 2 is a graph showing the effect on the spear formation of cells after chaga extract, licorice extract and red ginseng extract to HT-29 cells.

Claims (7)

Colorectal cancer prevention or treatment composition containing chaga extract as an active ingredient. The composition for preventing or treating colorectal cancer according to claim 1, wherein the composition for preventing or treating colorectal cancer further comprises one or more of licorice extract and red ginseng extract as active ingredients. According to claim 2, The chaga mushroom extract, licorice extract and red ginseng extract is a composition for preventing or treating colon cancer, characterized in that it comprises a weight ratio of 1 to 5: 1 to 10: 0.5 to 3. According to claim 1, wherein the chaga extract is colon cancer prevention or treatment composition, characterized in that the ethanol extract. The composition for preventing or treating colorectal cancer according to any one of claims 1 to 4, wherein the composition is used for a drug to assist the surgery before or after surgery for colorectal cancer. The composition for preventing or treating colorectal cancer according to any one of claims 1 to 4, wherein the composition is used in combination with another drug to assist the drug. The composition for preventing or treating colorectal cancer according to claim 2, wherein the red ginseng extract is a fermented red ginseng extract.

Images