KR20150072659A - Hepatoprotective composition containing smilax china extract - Google Patents

Abstract

The present inventino relates to hepatoprotective composition containing a smilax extract as an active ingredient. The present invention also relates to food composition for protecting a liver or improving a liver function or composition for curing or preventing a liver complaint, which contain a smilax extract as an active ingredient. The smilax extract does not have problem on safety or a side effect as a plant extract used for a edible ingredient and is confirmed to have effects of preventing a liver damage, improving a liver function, and protecting a liver in an experiment using animal model induced with hepatotoxicity or a cellular experiment. Therefore, the hepatoprotective composition also can be used in various purposes related to a fatigue recovery or hangover relieving.

Description

[0001] HEPATOPROTECTIVE COMPOSITION CONTAINING SMILAX CHINA EXTRACT [0002]

TECHNICAL FIELD The present invention relates to a plant extract having liver-protecting effect, specifically, an antimicrobial composition comprising a green tea extract as an active ingredient.

The liver is the most organs in the human body, the most active organ in the body. The liver is located between the digestive system and the systemic circulatory system and functions to defend the whole body from the exogenous substances introduced from the outside. In addition, the liver is a very important organ responsible for various metabolism, detoxification, degradation, synthesis and secretion.

More specifically, the liver has a function of managing energy metabolism, and all the nutrients absorbed from food are metabolized into a substance capable of producing energy in the liver and supplied or stored in the whole body, and about 2,000 kinds of enzymes, albumin, Factors such as serum protein, bile acids, phospholipids, fat such as cholesterol are synthesized, stored and distributed in the liver. In addition, the liver has a function of excreting various metabolites through the bile duct into the duodenum and an immune function, thus playing an important role in maintaining our life. In addition, hepatocytes are susceptible to damage due to detoxification of drugs, alcohol, and toxic substances in the liver as detoxification and decomposition functions, and thus, drug properties, toxicity, and alcoholic liver diseases are frequently caused. In addition, since the in vitro material enters the liver once, the liver has a higher risk of being exposed to many toxic substances than the other organs in addition to the nutrients.

Liver is an organ with excellent regeneration ability. It is fully recovered if there is some damage, but if the damage continues, part of the liver tissue is completely destroyed and liver function is also lowered. . If such liver damage is chronicized, liver fibrosis, cirrhosis, and liver cancer occur regardless of the cause. Liver fibrosis, liver cirrhosis, and liver cancer are diseases that do not have clear treatments and treatments at present and their mortality rates are also high. Therefore, preventing and treating such liver damage before liver damage is chronicized is crucial to inhibiting progression to liver fibrosis, cirrhosis, or liver cancer.

The liver disease is not asymptomatic at the beginning and the severity of liver damage is severe. For these reasons, the above-mentioned liver disease is the cause of death not only in Korea but also in the world, but there is no effective therapeutic agent and diagnostic method.

The liver disease can be classified according to the cause of the disease, but the liver has recently been increasingly concerned with liver disease.

Fatty liver is a cause of excessive intake of fat, increased accumulation and synthesis of liver, decrease of emission, etc., and normal lipid metabolism is not made, so lipids, especially triglyceride, accumulate excessively, .

Clinically, it is classified as fatty liver when fat is observed in 5% or more of hepatocytes or when fat is more than 5 mg per 100 mg of liver, that is, when fat accounts for more than 5% of total liver weight. In recent years, the number of patients with fatty liver has been rapidly increased due to the increase in living standards, the lack of exercise due to the development of high fat and high calorie diet, and the development of civilization, and the age group is on the whole from the 10th to the 50th or 60s. When the fatty liver is left untreated, it may progress to hepatitis, hepatopathy or liver cancer.

Alcoholic fatty liver disease and nonalcoholic fatty liver disease (NAFLD) caused by obesity, diabetes, hyperlipidemia or drugs can be categorized into fatty liver, have.

The alcoholic fatty liver disease progresses from an initial simple fatty liver to fatty liver, cirrhosis. Meanwhile, it has been known that the non-alcoholic fatty liver disease remains in the simple fatty liver and does not progress to the condition. However, it has recently been found that the nonalcoholic fatty liver disease also progresses from simple fatty liver to fatty liver or liver cirrhosis.

As described above, in recent years, patients with fatty liver have been on the increase due to improvement in nutritional status and increase in adult diseases due to lack of exercise. According to the Korean Hepatology Society, the prevalence of liver disease has declined over the past decade, but the prevalence of fatty liver has increased three-fold over 20 years. Among them, the ratio of non-alcoholic fatty liver exceeds 50% .

Non-alcoholic fatty liver disease is a disease characterized by fatty changes (steatosis) and lobular hepatitis (steatohepatitis), which are hallmarks of alcoholic hepatitis in liver biopsy despite the absence of alcohol- ) And so on. The pathologic findings of the liver include various non-alcoholic fatty liver disease (NAFL), non-alcoholic steatohepatitis (NASH), fibrosis associated with hepatitis, and liver cirrhosis. It is used to mean all inclusive.

The non-alcoholic fatty liver disease is mostly accompanied by complications such as insulin resistance, obesity, diabetes and hyperlipemia. In the presence of such complications, treatment should be performed. The principle of treatment for nonalcoholic fatty liver disease is improvement of lifestyle such as diet and exercise therapy, but it is a reality that it is difficult to perform reliably.

In addition, nonalcoholic steatohepatitis is more likely to progress to cirrhosis or hepatocellular carcinoma, so more active medication is needed.

Polyene phosphatidylcholine (Polyene Phosphatidylcholine) has been used clinically as a therapeutic agent for fatty liver and fibrate-based drug represented by clofibrate, which is a therapeutic agent for hyperlipidemia, has been used clinically as a therapeutic agent for fatty liver. It is believed that the fibrate drug improves lipid metabolism through liver-mediated beta-oxidase enzyme in the liver. However, fibrates are generally known to have side effects such as liver dysfunction.

Therefore, there is a need to develop a therapeutic agent for prevention and treatment of a new non-alcoholic fatty liver derived from a natural substance which is excellent in therapeutic effect but has no problems such as side effects and resistance.

KR 10-0953813 B KR 10-1076185 B KR 10-1139070 B

SUMMARY OF THE INVENTION The present invention has been made in order to solve the problems of the prior art as described above, and it is an object of the present invention to provide a composition for treating or preventing fatty liver, specifically nonalcoholic fatty liver disease, And to provide a functional food composition having a preventive effect.

In order to attain the above object, the present invention provides a composition for protecting the liver, which comprises the Phellodendri shower extract as an active ingredient.

In addition, the present invention provides a composition for treating or preventing liver diseases, which comprises a Cheongmyeong extract as an active ingredient.

In order to achieve the above object, the present invention also provides a functional food composition for improving liver function and hepatic function, which comprises Chengmyeong extract as an active ingredient.

The inventors of the present invention have found that when a composition for protecting liver derived from a natural substance which does not cause a problem of stability such as side effects or resistance is used, it can be used as an edible food and its stability is confirmed by using a Chengmira extract. The present inventors have completed the present invention.

Hereinafter, the present invention will be described in more detail.

The present invention relates to a composition for protecting the liver comprising an extract of Cheongpyeong as an active ingredient.

The liver protecting composition may be a pharmaceutical composition or a food composition. In view of the above, the present invention relates to a composition for treating or preventing liver diseases, which comprises a Cheongmyeong extract as an active ingredient. The present invention also relates to a functional food composition for preventing liver damage, improving liver function, and / or preventing liver damage, which comprises Chengmyeong extract as an active ingredient.

Smilax china is a vine-shrub with lilies. It is a thornbush tree commonly found in southern Hwanghae provinces. Its roots are thick and serpentine. The leaves are round, round and shiny green with marginal edges. The stipule on the base of the leaf is made of a whisker. The flowers of the bluefin sunflower are yellow in the late spring when the male and the female are yellow, and there are 6 horns, 6 stamens, one pistil, and red fruits.

The above fruits are called senses or palms and they are used by children, young seeds are eaten as herbs, and leaves are used to make daikoku rice cake or wrap rice cake. The roots are called soil roots and are used as medicinal materials. The roots of Chengmyeong have diuretic action and anti-inflammatory action and have been used as therapeutic agents for enteritis, dysentery, lymphadenitis and syphilis.

The above-mentioned Cheongmyeong Extract may be prepared by a conventional method for producing a plant extract. For example, the Cheongmyeong Extract may be prepared by extracting an extracting solvent into fruit, flower, leaf, branch, stem, root, Or may be one prepared by fractionating a crude extract prepared by extracting with a solvent by adding a fraction solvent.

The extraction solvent may be at least one selected from the group consisting of water and an organic solvent. The organic solvent may be a polar solvent such as an alcohol having 1 to 5 carbon atoms, a diluted alcohol, ethylacetate or acetone, a nonpolar solvent such as ether, chloroform, benzene, hexane or dichloromethane, or a mixture thereof. The alcohol having 1 to 5 carbon atoms may be methanol, ethanol, propanol, butanol, or isopropanol, but is not limited thereto. The alcohol diluted water may be an alcohol diluted with water at 50 to 99.9% (v / v).

The extraction solvent of the Chengmyeong extract of the present invention may be at least one selected from the group consisting of water, an alcohol having 1 to 5 carbon atoms, an alcohol-diluted water, and a mixture thereof, more preferably water, Alcohol, and a mixture thereof. The extraction process may be performed at, for example, 50 ° C to 150 ° C, or 75 ° C to 120 ° C, or 90 ° C to 110 ° C, but is not limited thereto. The extraction time is not particularly limited, but may be 10 minutes to 12 hours, or 30 minutes to 6 hours, or 2 hours to 4 hours.

The cheongmyeong extract of the present invention may be one prepared by a conventional method for producing a plant extract. Specifically, it may be a heat extraction method including a hot water extraction method, a cold extraction extraction method, a warm extraction extraction method, an ultrasonic extraction method, An extractor or a fractionator may be used.

The extract obtained by the solvent is then subjected to fractionation with any one solvent selected from the group consisting of butanol, hexane, methylene chloride, acetone, ethyl acetate, ethyl ether, chloroform, water and mixtures thereof, preferably butanol Can be further carried out. Two or more kinds of solvents may be used for the fractionation, and each solvent extract may be prepared by sequentially using or mixing the solvents according to the polarity of the solvent

The thus-prepared extract or the fraction obtained by performing the fractionation process may be filtered, concentrated or dried to remove the solvent, and may be subjected to both filtration, concentration and drying. Specifically, the filtration can be performed using a filter paper or a vacuum filter, and the concentration can be reduced by using a vacuum concentrator, for example, a rotary evaporator. The drying can be performed by, for example, freeze drying.

It was confirmed that the present Chengmyeong extract has a remarkably excellent liver protecting effect in a cell test or an animal model of hepatotoxicity induced hepatotoxicity.

Therefore, the composition for protecting the liver comprising the Chengmyeong extract and the Chengmyeong extract as an active ingredient can be used for treatment or prevention of liver disease, protection of liver, improvement of liver function and prevention of liver damage.

The above-mentioned liver disease is a concept including general liver disease, and may be one or more selected from the group consisting of hepatotoxic disease, liver damage, drug liver damage, hepatitis, liver cirrhosis, liver cancer and hepatic coma.

Preferably, the liver disease is any one selected from the group consisting of alcoholic liver disease and liver toxic liver disease. In addition, the liver protection, hepatic function improvement or hepatic injury prevention effect can be applied as a fatigue recovery effect or a hangover resolution effect.

Therefore, the liver protecting composition of the present invention can be applied as a composition for treating or preventing liver disease, or as a functional food composition for protecting the liver, for improving liver function, for preventing liver damage, for preventing fatigue, and / have. The food composition may be, for example, a health functional food composition for liver protection and liver function improvement, a health functional food composition for fatigue recovery, or a health functional food composition for hangover resolution.

The above-mentioned health functional foods are used to control the bio-defense rhythm of the food group or the food composition imparted with added value to function and express the function of the relevant food by physical, biochemical and biotechnological techniques, Means a food which is processed and designed so that the body controlling function of the body is sufficiently expressed to the living body. The health functional food may include food-acceptable food supplementary additives, and may further include suitable carriers, excipients and diluents conventionally used in the production of health functional foods.

The composition for protecting the liver of the present invention may contain 0.001 to 99.9% by weight or 0.01 to 50% by weight or 0.1 to 30% by weight or 0.1 to 15% by weight of the whole of the composition.

The composition for protecting the liver comprising the above-described Cheongmyeong extract as an active ingredient can be directly applied to animals including humans. The animal is a group of organisms corresponding to plants. It mainly consumes organic matter as nutrients, and differentiates digestive organs, excretory or respiratory organs. Preferably, it is a mammal, more preferably a human.

The Cheongmyeong Extract may be used alone in the liver protecting composition, and may further include other pharmacologically acceptable carriers, excipients, diluents or subcomponents. More specifically, when the composition containing the Chengmyeong extract is used as a medicine or used for medicinal or pharmaceutical purposes, the Chengmyeong extract is mixed with a pharmaceutically acceptable carrier or excipient according to a conventional method, or mixed with a diluent It can be used by dilution.

In this case, the content of the Cheongmyeong extract in the composition may be 0.001 wt% to 99.9 wt%, 0.1 wt% to 99 wt%, or 1 wt% to 50 wt%, but is not limited thereto. The content of the compound may be appropriately adjusted in a desired amount.

Examples of the pharmaceutically acceptable carrier, excipient or diluent include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, Cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil, propylhydroxybenzoate, talc, magnesium stearate and mineral oil , Dextrin, calcium carbonate, propylene glycol, liquid paraffin, and physiological saline. However, the present invention is not limited thereto, and any conventional carrier, excipient or diluent may be used. In addition, the pharmaceutical composition may further comprise at least one selected from the group consisting of conventional fillers, extenders, binders, disintegrants, anticoagulants, lubricants, humectants, pH adjusters, nutrients, vitamins, electrolytes, alginic acid and its salts, pectic acid and its salts, , Fragrances, emulsifiers, preservatives, and the like. The components may be added to the Cheongfwai extract as the active ingredient independently or in combination.

In addition, the composition of the present invention can be used as a substance recognized as having a therapeutic effect of liver disease, liver protection effect, liver function improvement, etc., in addition to the above-mentioned effective ingredient, for example, a substance for protecting hepatocytes, a liver disease treatment agent, Lt; / RTI >

When the composition is used as a medicine, it can be administered orally or parenterally. In one example, it can be administered through various routes including oral, transdermal, subcutaneous, intravenous or muscular.

In addition, the formulations of the compositions may vary depending on the method of use and may be formulated using methods well known in the art to provide rapid, sustained or delayed release of the active ingredient after administration to the mammal . In general, solid dosage forms for oral administration include tablets, pills, soft or hard capsules, pills, powders and granules, which may contain one or more Excipients such as starch, calcium carbonate, sucrose or lactose, gelatin, and the like. In addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. Liquid preparations for oral use include suspensions, solutions, emulsions, and syrups. In addition to water and liquid paraffin, which are commonly used simple diluents, various excipients such as wetting agents, Sweeteners, fragrances, preservatives, and the like.

Forms for parenteral administration include creams, looses, ONITMENTS, PLASTERS, LIQUIDS AND SOULTIONS, AEROSOLS, FRUIDEXTRACTS, (ELIXIR), INFUSIONS, SACHET, PATCH or INJECTIONS.

Furthermore, the compositions of the present invention may be formulated using any suitable method known in the art or using methods disclosed in Remington's Pharmaceutical Science (recent edition), Mack Publishing Company, Easton PA .

The dosage of the composition may be determined in consideration of the administration method, the age, sex, the severity of the patient, the condition of the patient, the degree of absorption of the active ingredient in the body, the inactivity and the drug to be used in combination. Kg body weight to 500 mg / kg body weight, 0.1 mg / kg body weight to 400 mg / kg body weight or 1 mg / kg body weight to 300 mg / kg body weight, And may be administered once or several times in divided doses. The dose is not intended to limit the scope of the invention in any way.

The present invention also provides a composition for the treatment or prevention of liver diseases, which comprises the above-described Cheongmyeong extract as an active ingredient or an effective component thereof.

The above-mentioned liver disease is a concept including general liver disease, and may be one or more selected from the group consisting of hepatotoxic disease, liver damage, drug liver damage, hepatitis, liver cirrhosis, liver cancer and hepatic coma. In addition, the liver protection, hepatic function improvement or hepatic injury prevention effect can be applied as a fatigue recovery effect or a hangover resolution effect.

The composition for the treatment or prevention of liver diseases can protect liver and liver cells from liver damage caused by external requirements such as liver protection, for example, toxic damage or internal requirements in the body, and thus is used for prevention and treatment of liver diseases It is possible.

The above composition for treating or preventing liver disease may contain the active ingredient alone, and may further include a pharmaceutically acceptable carrier or excipient depending on the formulation, the method of use, and the intended use. When provided as a mixture, the active ingredient may be contained in an amount of 0.1 to 99.9% by weight based on the whole composition, but is usually contained in an amount of 0.001 to 50% by weight.

Examples of the carrier or the excipient include water, dextrin, calcium carbonate, lactose, propylene glycol, liquid paraffin, physiological saline, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gelatin, calcium phosphate, But are not limited to, calcium silicate, cellulose, methylcellulose, polyvinylpyrrolidone, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. Two or more carriers or excipients may be used.

In addition, when the composition for treating or preventing liver diseases is weakened, it may further contain conventional fillers, extenders, binders, disintegrants, surfactants, anti-coagulants, lubricants, wetting agents, flavors, emulsifiers or preservatives.

In addition, the composition for treating or preventing liver disease of the present invention may further comprise a substance containing a compound or plant extract having a known liver disease prevention or therapeutic activity or liver protecting activity in addition to the effective ingredient, And 5 to 20 parts by weight per 100 parts by weight of the composition.

The formulation of the composition for the treatment or prevention of liver diseases may be in a desired form depending on the method of use and may be selected so as to provide rapid, sustained or delayed release of the active ingredient after administration to the mammal, . Exemplary formulations include, but are not limited to, excipients, granules, lotions, liniments, rimonadense, powders, syrups, ointments, liquids, aerosols, EXTRACTS, elixirs, ointments, Suspensions, premixes, infusions, eye drops, tablets, suppositories, injections, main tablets, capsules, creams, pills, soft or hard gelatin capsules.

The composition for treating or preventing liver diseases according to the present invention may be used orally or parenterally and may be used, for example, through the intradermal, intramuscular, intraperitoneal, intravenous, subcutaneous, intramuscular, epidural, The dosage thereof is preferably determined in consideration of the administration method, the age, sex, and weight of the recipient, the severity of the disease, and the like. For example, the composition for treating or preventing liver diseases according to the present invention may be administered at least once at a dose of 0.1 to 100 mg / kg (body weight) per day based on the active ingredient. However, the above-mentioned dosage is merely an example for illustrative purposes and is not limited to the above range.

The present invention also provides a composition for improving liver function and liver function comprising an effective amount of the above-described Cheongmyeong extract as an active ingredient or an extract of Cheongmyeong as an active ingredient.

The food composition may be a functional food composition or a health functional food. The food composition may be a health functional food composition for liver protection and liver function improvement, a health functional food composition for fatigue recovery, or a health functional food composition for hangover resolution Can be applied.

The term "food composition" as used herein means a natural product or a processed product containing one or more nutrients. For example, the food composition may mean that it can be directly eaten through a certain degree of processing, Which is intended to include food, food additives, health functional foods and beverages.

Foods to which the active ingredient of the present invention can be added include, for example, various foods, beverages, gums, tea, vitamin complexes, and functional foods. In addition, in the present invention, the food may be selected from the group consisting of special nutritional foods (eg, crude oil, spirit, infant food, etc.), meat products, fish products, tofu, jelly, noodles (Such as soy sauce, soybean paste, hot pepper paste, mixed sauce), sauces, confectionery (eg snacks), dairy products (eg fermented milk, cheese), other processed foods, kimchi, pickled foods But are not limited to, fruits, vegetables, beverages, fermented beverages, etc.), natural seasonings (e.g., ramen soup, etc.). The food, beverage or food additive may be prepared by a conventional production method.

In the present invention, the functional food refers to a food group which is imparted with added value to function and express the function of the food by using physical, biochemical, biotechnological techniques and the like, the regulation of the biological defense rhythm of the food composition, Means a food which is processed and designed so that the body control function regarding the body is sufficiently expressed to the living body. The functional food may include a food-acceptable food-aid additive, and may further comprise suitable carriers, excipients and diluents conventionally used in the production of functional foods.

In the present invention, beverage is a generic term for drinking or enjoying a taste, and is intended to include functional beverages. The beverage is not particularly limited as long as it contains the above-described Phellinus lint extract or liver protecting composition as an active ingredient as an essential ingredient at the indicated ratio, and it is also possible to add various flavors or natural carbohydrates, ≪ / RTI > Examples of such natural carbohydrates include monosaccharides such as glucose, fructose, and the like; Disaccharides such as maltose, sucrose, and the like; And polysaccharides, for example, conventional sugars such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. Natural flavors (tau martin, stevia extract (e.g., rebaudioside A, glycyrrhizin, etc.) and synthetic flavors (saccharin, aspartame, etc.) can be advantageously used as flavors other than those described above The ratio of the natural carbohydrate is generally about 1 g to 20 g or 5 g to 12 g per 100 ml of the composition of the present invention In addition, the composition of the present invention can be used for the production of natural fruit juice, fruit juice drink, Can be added.

In addition to the above-mentioned composition, the composition of the present invention can be used as a flavoring agent such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, coloring agents and intermediates (cheese, chocolate etc.), pectic acid and its salts, Salts, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated beverages and the like. These components can be used independently or in combination. The proportion of such an additive is not so critical, but may be selected in the range of 0 to 200,000 parts by weight per 100 parts by weight of the composition of the present invention.

The functional beverage according to the present invention can be used to control the bio-defense rhythm of the beverage group or beverage composition to which the added value is imparted so that the function of the beverage acts on the specific purpose by physical, biochemical or biotechnological techniques, Means a beverage which has been designed so that the body control function related to restoration and the like is sufficiently expressed in the living body.

The functional beverage is not particularly limited to the other components other than the active ingredient of the composition of the present invention as an essential ingredient in the indicated ratio and may contain various flavors or natural carbohydrates as an additional ingredient such as ordinary beverages . Examples of such natural carbohydrates include monosaccharides such as glucose, fructose, and the like; Disaccharides such as maltose, sucrose, and the like; And polysaccharides, for example, conventional sugars such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. Natural flavors (tau martin, stevia extract (e.g., rebaudioside A, glycyrrhizin, etc.) and synthetic flavors (saccharin, aspartame, etc.) can be advantageously used as flavors other than those described above The ratio of the natural carbohydrate may generally be about 1 g to 20 g or 5 g to 12 g per 100 ml of the composition of the present invention.

In the above food composition for improving liver protection and liver function, the content of the active ingredient is preferably 0.0001 to 99% by weight or 0.001 to 50% by weight or 0.01 to 30% by weight or 0.1 To 15% by weight of the beverage composition, and the beverage composition may contain 0.0002 g to 5 g or 0.03 g to 1 g based on 100 ml.

In addition, the intake amount of the food composition for liver protection and hepatic function improvement varies depending on the condition and body weight of the individual to be ingested, the severity of the disease, the drug form, the administration route and the period, but can be appropriately selected by those skilled in the art. For example, the food composition of the present invention may contain 0.0001 g / kg (amount of active ingredient / weight) to 12 g / kg (amount of active ingredient) per day of active ingredient or 0.01 g / kg to 9 g / kg. < / RTI > The method of administration may be administered once a day, divided into several doses, and the dosage and method of administration are not intended to limit the scope of the present invention in any aspect.

The plant-derived extract used as the active ingredient of the present invention, Pungmira extract, has no problems with regard to side effects or safety, and as a result of the experiment using the Pungmira extract, it has excellent effects on the treatment and prevention or improvement of liver protection and liver diseases Respectively.

Therefore, the liver protecting composition of the present invention comprising the Cheongmyeong Extract as an active ingredient can be used for a variety of uses related to liver protection and liver function such as a pharmaceutical composition for treating or preventing liver disease, a food composition for improving liver function or liver protection, And the like. Therefore, the present invention can be widely used in the fields of medical industry and food industry related to liver protection, liver function improvement and treatment or prevention of liver disease.

Hereinafter, the structure and effects of the present invention will be described in more detail with reference to specific examples and comparative examples in order to facilitate understanding of the present invention. However, it should be understood that the following examples are for the purpose of further illustrating the present invention and are not to be construed as limiting the scope of the invention as defined by the appended claims. All technical ideas are to be construed as falling within the scope of the present invention.

< Example  1> Cell culture and preparation of experimental material

Extracts of 2,000 g each of roots, fruits, leaves and stems of Smilax china were subjected to hot water extraction at 100 ° C using 40 L of hot water to prepare hot water extracts.

More specifically, 40 L of distilled water was added to 2,000 g of each of the roots, fruits, leaves and stems washed with water, and then subjected to hot water extraction while heating at 100 占 폚 for 3 hours using an electric potter. After the extraction was performed, the filtrate was filtered through a 400-mesh filter cloth, and the filtrate was concentrated using a rotary evaporator under reduced pressure. After filtration, the same amount of distilled water was again added to the remaining residue, and the same filtration and decompression procedures were repeated two more times. The hot-water extract solution of each part of Cheongpyonglae prepared by the above procedure was freeze-dried in a freeze dryer. Through the lyophilization, a hot water extract of Cheongpyonghae was obtained.

The fractions of the above-mentioned water-extract of Chungmeira were prepared by completely dissolving 40 g of the hot-water extract in 25-fold distilled water, that is, 1 L of distilled water, adding to 1 L of hexane, adding 1 L of hexane, (Water layer) was added to 1 liter of chloroform. After mixing, the chloroform layer was separated from the chloroform-insoluble layer, and the chloroform layer was separated from the chloroform layer. , And 1 L of ethyl acetate was added to the remaining solution (water layer), followed by fractionation to separate an ethyl acetate layer and an aqueous layer as an ethyl acetate insoluble layer to obtain an ethyl acetate layer, thereby obtaining ethyl Acetate fraction, and 1 L of butanol was added to the remaining solution (water layer), followed by mixing To separate the butanol layer and the aqueous layer as the butanol insoluble layer and obtain only the butanol layer to prepare a butanol fraction solution. Each of the obtained fraction fractions was filtered through a filtration apparatus under reduced pressure, concentrated at -20 ° C The solvent was completely removed by lyophilization and used in this experiment.

The extracts and fractions obtained above were stored frozen until used in the experiment.

Manufacturing example : Cheongmaerae  Preparations using extracts

Manufacturing example  One. Sanje  Produce

Cheongmaeae extract 2 mg

Lactose 100 mg

Talc 10 mg

The above ingredients were mixed and filled in an airtight container to prepare powders.

Manufacturing example  2. Preparation of tablets

Cheongmaeae extract 2 mg

Corn starch 100 mg

Lactose 100 mg

Magnesium stearate 2 mg

After mixing the above components, tablets were prepared by tableting according to the usual preparation method of tablets.

Manufacturing example  3. Preparation of capsules

Cheongmaeae extract 2 mg

Crystalline cellulose 3 mg

Lactose 14.8 mg

Magnesium stearate 0.2 mg

The above components were mixed according to a conventional capsule preparation method and filled in gelatin capsules to prepare capsules.

Manufacturing example  4. Liquid  Produce

Cheongmaeae extract 2 mg

10 g per isomer

5 g mannitol

Purified water quantity

Each component was added to purified water in accordance with the usual liquid preparation method and dissolved, and the lemon flavor was added in an appropriate amount. Then, the above components were mixed, and purified water was added thereto. The whole was adjusted to 100 ml with purified water, And sterilized to prepare a liquid preparation.

Manufacturing example  5. Manufacture of health functional foods

Cheongmaeae extract 2 mg

Vitamin mixture quantity

70 [mu] g of vitamin A acetate

Vitamin E 1.0 mg

0.13 mg vitamin B1

0.15 mg of vitamin B2

0.5 mg vitamin B6

0.2 [mu] g vitamin B12

10 mg vitamin C

Biotin 10 μg

Nicotinic acid amide 1.7 mg

50 ㎍ of folic acid

Calcium pantothenate 0.5 mg

Mineral mixture quantity

1.75 mg of ferrous sulfate

0.82 mg of zinc oxide

Magnesium carbonate 25.3 mg

15 mg of potassium phosphate monobasic

Secondary calcium phosphate 55 mg

Potassium citrate 90 mg

100 mg of calcium carbonate

24.8 mg of magnesium chloride

Although the composition ratio of the above-mentioned vitamin and mineral mixture is comparatively mixed with a component suitable for a health functional food as a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above components may be mixed And then granules are prepared and used in the manufacture of health functional foods according to conventional methods.

Claims (3)

A pharmaceutical composition for the treatment or prevention of any one of the liver diseases selected from the group consisting of alcoholic liver diseases and hepatotoxic liver diseases, which contains Cheongmyeong extract as an active ingredient. Wherein the composition is selected from the group consisting of alcoholic liver diseases and hepatotoxic liver diseases, which comprise Cheongmyeong Extract as an active ingredient. 3. The method of claim 2,
Wherein the food composition is a health functional food composition.