WO1995018626A1 - Medicament tonique a base de basidiomycetes, d'algues et d'herbes officinales chinoises, et son procede de preparation - Google Patents

Medicament tonique a base de basidiomycetes, d'algues et d'herbes officinales chinoises, et son procede de preparation Download PDF

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WO1995018626A1
WO1995018626A1 PCT/CN1994/000070 CN9400070W WO9518626A1 WO 1995018626 A1 WO1995018626 A1 WO 1995018626A1 CN 9400070 W CN9400070 W CN 9400070W WO 9518626 A1 WO9518626 A1 WO 9518626A1
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bran
puree
following weight
mushroom
kelp
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PCT/CN1994/000070
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English (en)
Chinese (zh)
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Lushan Lin
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Lushan Lin
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Priority to AU75300/94A priority Critical patent/AU7530094A/en
Publication of WO1995018626A1 publication Critical patent/WO1995018626A1/fr

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/47—Hydrolases (3) acting on glycosyl compounds (3.2), e.g. cellulases, lactases
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/02—Algae
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/06—Fungi, e.g. yeasts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/465—Hydrolases (3) acting on ester bonds (3.1), e.g. lipases, ribonucleases
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/48—Hydrolases (3) acting on peptide bonds (3.4)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/48—Hydrolases (3) acting on peptide bonds (3.4)
    • A61K38/482—Serine endopeptidases (3.4.21)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/02—Nutrients, e.g. vitamins, minerals

Definitions

  • the invention relates to a biological fermentation product and a preparation method thereof, in particular to a health care medicine obtained by using a fermentation fermentation technology and a preparation method thereof.
  • the purpose of the present invention is to overcome the shortcomings of the prior art, and to provide a biological fermentation product with multiple health-enhancing and therapeutic effects, which is mainly composed of fungi, seaweed and Chinese herbal medicine ⁇
  • Another object of the present invention is to provide a method for preparing the above-mentioned biological fermentation product, so as to avoid the disadvantageous color noise generated by using traditional Chinese medicine processing methods, such as high temperature heating.
  • the present invention relates to a biological fermentation product, which comprises the fruit bodies and seaweeds of Basidiomycetes 32-48 * based on the total weight of the main family, and 52-68 * Chinese herbal medicines based on the original protoplast, bacterial oxalase, and Fermentation products with enzyme-promoting agent, carbon source, and nutritional supplements as supplementary raw materials, of which:
  • the weight ratio of the basidiomycete to the seaweed is 2: 0. 5-1. 5;
  • the basidiomycete is selected from one or more kinds of lentinus, edulis, Poria, Hog, Poria, Ganoderma lucidum, Hericium erinaceus, mushroom, Tremella;
  • the seaweed is selected from one or two of kelp, laver, seaweed, and hairweed;
  • the bacterial oxalase is selected from one or more of ⁇ -amylase, cellulase, pectinase, and fungal protease, and the added rhenium is 0.01-0.0 of the total weight of the raw materials;
  • the enzyme-promoting agent is selected from yeast mash or corn slurry or a mixture thereof;
  • the nitrogen source and carbon source are selected from the group consisting of peptone, bran, fine bran, sweet potato flour, corn flour, bran, rice bran, One or more of mashed potato powder, tulipa powder, bean cake powder, peanut cake powder, rapeseed cake powder, black sesame powder, brown sugar, and white sugar;
  • the nutritional additives are inorganic salts and substances combined with phosphorus, calcium, potassium, magnesium, selenium, and zinc; the weight ratio of the main raw material to the fermentation promoter, nitrogen source, carbon source, and nutritional additive is 100: 0.02 -0.1: 0.18-0.22: 4.6-5.4: 0.14-0.16.
  • the method for preparing a biological fermentation product of the present invention includes the following steps:
  • the main drug source of the drug is taken, which includes 32-48 weight percent of fungi and seaweed, and 52-68 weight percent of Chinese herbal medicine; bacterial oxalase; the enzyme production promoter, nitrogen source, carbon source and Nutritional additives as pharmaceutical auxiliary materials;
  • the choice of various kinds of raw materials and their amounts have the same definition as above.
  • the various bacterial oxalase enzymes are prepared as follows:
  • the 0! -Amylase is derived from corn starch 2, corn slurry 26 *, disodium hydrogen phosphate 37 *, calcium fluoride
  • the fungal protease is derived from corn starch 303 ⁇ 4, corn slurry 5 *, soy flour 53 ⁇ 4, casein gelatin dried distiller's grains 53 ⁇ 4, dipotassium phosphate 2.4%, sodium nitrate 1 ammonium gasification: ⁇ , iron sulphate 0.01 bran 5 *, Fine bran 5 *, culture product of Ganoderma spore pink koji 1 ⁇ 2, wherein the percentage is weight percentage;
  • the cellulase is derived from edible fungus green waste fungus 653 ⁇ 4 ⁇ , bran 10%, bran 10 lucic acid Solid fermentation product of ammonium 2.5%, water 250%, yeast expensive 1 Tween 80.0.05%, crude brown sugar 1 red yeast rice 1 *, the percentage of which is weight percentage;
  • bran 0.5 ammonium sulphate 0.2 potassium dihydrogen phosphate 0.1, calcium carbonate 0.1%, glucomannose 1 * in a triangle flask, 30 e C, 104 rpm, culture 32-35 hours The first-stage fermentation product of the seed was obtained; the waste fungus material 6 bran 0.53 ⁇ 4, glucosamine ammonium gluconate 0.2% potassium dihydrogen phosphate 0.1% were at 30 e C, 104 Turn / min and culture for 60 hours to obtain a secondary seed culture; seed primary and secondary cultures, peptone 0.075%, Tween 80 0.2%, ammonium sulphate 1.4g / I potassium dihydrogen phosphate S.
  • the pectinase is derived from 4% of pectin, 0.4% of peptone, 0.05% of sodium sulphate, 0.01% of iron sulphate, 0.05% of magnesium sulphate, 0.05% of potassium vaporization, 55% of potassium dihydrogen phosphate bran, 5% fine bran, sucrose 2%, ammonium nitrate 0.2%, sodium nitrate 0.2 and connected to the spore suspension wave of ganoderma spores containing 2 10 3 spores, 30-C, culture product obtained by culturing in a conical flask for 7 days, where the percentage is weight percentage .
  • the above preparation method of the present invention enables some ingredients in Chinese herbal medicines to be changed during the preparation process, that is, the molecular structure of the drug components is changed, so that it can most effectively exert the persuasion of the original subjects, so that the prepared medicine has more It is suggested that health care can be effective, and some difficult diseases of Meike are treated, and the preparation steps are simple and easy.
  • the biological fermentation product of the present invention is preferably the following combined fermentation product of the original family, and each component used in the preparation method thereof has the following weight percentages based on the total weight of the raw materials:
  • the basidiomycetes and seaweeds have the following weight percentages: Lentinus edodes 7-83 ⁇ 4, Flammulina velutipes 18-193 ⁇ 4, Poria 1-2%, Ganoderma 2-3 kelp 14-163 ⁇ 4;
  • the Chinese herbal medicine has the following weight percentage components: Boziren 7-8 *, Shezizi 3-4 Cuscuta 3-4 Raspberry 3-4%, Dendrobium 2-3%, Morinda officinalis 1-2, Continued off 4-5 Black medicine 0.5-1%, Calamus 1-1.5%, Alisma 0.5-1%, Chinese yam 1-2 ginseng 1-2%, 'Rehmannia glutinosa 2- 3 mulberry parasitic 0.5-1%, Dogwood 1-1 Schisandra 3-5%, stalactites 1-23 ⁇ 4, Cistanche 2-3% and ox vine 0.7-13 ⁇ 4 ⁇ ;
  • the auxiliary raw materials are components with the following weight percentages: peptone 0.01-0.02%, brown sugar 0.2-0.3%, stone powder: 1, KCI 0.4-0.5%, MgSO 4 0. -0.5%, KH 2 PO 4 0.4-0.5%,
  • the bacterial oxalase ⁇ fiber's enzyme produced by the above method is the bacterial oxalase ⁇ fiber's enzyme produced by the above method.
  • the mixture of basidiomycete puree and / or seaweed puree used for inoculation is: Enoki mushroom puree, mushroom puree, Ganoderma lucidum and kelp puree with a weight ratio of 2: 1.
  • the basidiomycetes and seaweeds have the following weight percentages: Shiitake mushrooms 7-8%, Enoki mushroom 4-6 soil Poria 1-23 ⁇ 4, Ganoderma 2-33 ⁇ 4, kelp 7-8%;
  • the Chinese herbal medicine has the following weight percentage components: Bo Zi Ren 4-5 *, She Chuang Zi 4-4.5 ⁇ Silk seeds 4-4.5%, raspberries 4-4.5 ⁇ fe, Dendrobium 1-1.53 ⁇ 4 ;, Morinda officinalis 1.-1.5%, Eucommia ulmoides 1-1.5 Asparagus 1-1.5%, Zhiyuan (heart removal) 1 : 1.5 1 ⁇ 2: Zhiren 0.2-0.5 continuity 0.5-0.73 ⁇ 4 ;, Wuya 0.5-0.7 alfalfa 0.7-1 Alisma 0.7-1S ;, yam 0.8- ⁇ , ginseng 0.8-1 rehmannia 0.5-0.8 dogwood S.6- 1%, Five Gardenias 2-3 Stalactites (refined) 1-1.23 ⁇ 4 ;, Cistanche 2-3
  • the auxiliary progenitors are components having the following weight percentages: bran 7-8%, fine bran 7-8 sweet potato powder 4-4.2%, yeast 1-1.23 ⁇ 4f, sucrose, calcium carbonate 1-1.2
  • the bacterial oxalase is a cellulase made by the above method.
  • the mixture of basidiomycete puree and / or seaweed puree used for inoculation is: Enoki mushroom puree, mushroom puree, Ganoderma lucidum and kelp puree with a weight ratio of 2: 1.
  • the basidiomycetes and seaweeds have the following weight percentages: Poria cocos, Potentilla 7-83 ⁇ 4, kelp 7-83 ⁇ 4;
  • the Chinese herbal medicine has the following weight percentage components: Polygonum multiflorum (manufactured) 5-6%, Achyranthes bidentata 3-43 ⁇ 4, Cuscuta 3-4, Ligustrum 3-4%, Eucommia ulmoides 4-5, County sign grass 5- 6%, mulberry leaves 2-3%, silver flowers 3-4%, black mulberries 3-4%, medlar 0.3-0., Chrysanthemums 5 -6% Dihuang 3 _43 ⁇ 4, mangosteen 3-43 ⁇ 4, tannin 3 _ 4 %, Alisma 0.3-0.43 ⁇ 4, yam 3-4%;
  • the auxiliary raw tincture is a component having the following weight percentages: bran 7-, fine bran 7-8%, yeast 1-1.2%, sucrose 1-1.2%, calcium carbonate 1-1.2 *;
  • the bacterial oxalase is 0.02 * cellulase and 0.01% pectinase;
  • the mixture of basidiomycete puree and / or seaweed puree used for inoculation is: shiitake mushroom puree, mushroom puree, kelp puree.
  • the basidiomycetes and seaweeds have the following weight percentages: Lentinus 3-, Mushroom 3-4%, Hericium 3- «, Kelp 5-6%;
  • the Chinese herbal medicine has the following weight percentage components: Huangyaozi 10-20 *, Chuandi 4-5 *, Shayuanzi 4-5, Oyster 3-43 ⁇ 4, Curcuma 3-4%, Coix Seed 3-, Chuanxiongzi 4 -5 green peel 3 -4%, ⁇ 1.2-1.5%, spot ⁇ 1.2-1.5 *, talc 3-43 ⁇ 4 ⁇ , unicorn 3-4 amomum 2-33 ⁇ 4 !, chicken gizzard inside 2-3%, codonopsis 2 -3%, astragalus 3-63 ⁇ 4, cooked rehmannia 3-6 *, 'er ug 3-61 ⁇ 2, betel nut slices 3-6%, Sichuan army 1-2 yuan Ming powder 1-2 dried toad 0.5-1 *, acute 0.6 -1, 2 Zhuru 2 ⁇ 3 ⁇ 3 ⁇ 4 and ochre 4-5 *;
  • the auxiliary raw materials are components having the following weight percentages: bran 4-5 fine bran 4-5%, yeast 1.2-1.5%, brown sugar 3-, soybean powder 3-, calcium carbonate 1-1.2%;
  • the bacterial oxalase is 0.023 ⁇ 4 of the above-mentioned cellulase and 0.01% of the above-mentioned pectinase;
  • the mixture of basidiomycete puree and / or seaweed puree used for inoculation is: shiitake mushroom puree, kelp puree, and ganoderma puree.
  • the basidiomycetes and seaweeds have the following weight percentages: 10-15% Tremella, 1.5-4.5 Shiitake 5-;
  • the Chinese herbal medicine has the following weight percentage components: Xuanshen 3-, Ophiopogon 3-, Raw Rehmannia 3-4%, Chiba 1-2 Danshen 1-2S ;, Astragalus 3-4%, Yam 3- , Coix 1-2 *, Safflower 1_2 *, Bupleurum 0.5-5-0. 6 * and sea cucumber 7-8%;
  • the auxiliary raw materials are components having the following weight percentages: bran fine bran 6-8 sweet potato powder 6-8%, yeast 1.2-23 ⁇ 4, sucrose 1.2-2%, calcium carbonate 1-1.2
  • the bacterial oxalase is 0.02 * of the above-mentioned cellulase and 0.01% of the above-mentioned pectinase;
  • the mixture of basidiomycete puree and / or seaweed puree used for inoculation is: shiitake mushroom puree, kelp puree, and ganoderma puree.
  • the basidiomycetes and seaweeds have the following weight percentages: Lentinus 2-3, Poria cocos 1-23 ⁇ 4, Poria cocos 4-5%, Kelp 4-5;..
  • the Chinese herbal medicine has the following weight percentage components: Astragalus 1-2, Codonopsis 1-2 *, Fangfang 1-2, Atractylodes 1-2, Shouwu 2-4 *, Zexie 4-8, Hawthorn 2-4, Yinchen 2-4%, buffalo horns 2-4 *, epimedium 2-4 * and rhubarb
  • the auxiliary raw materials are components having the following weight percentages: bran 10-12%, fine bran 5-63 ⁇ 4, sweet potato flour 5-6%, baker's yeast 1-1.2 brown sugar 1-1.2 *, calcium carbonate 1-1.2 *;
  • the bacterial oxalase is 0.0023 ⁇ 4; the above-mentioned cellulase O. 01 * ’s fungal protease; the mixture of basidiomycete puree and / or seaweed puree used for inoculation is: shiitake puree, Mushroom puree, seaweed puree.
  • the basidiomycetes and seaweeds have the following weight percentages: shiitake mushrooms 15-20%, kelp
  • the Chinese herbal medicine has the following weight percentage components: Polygonum multiflorum 18-223 ⁇ 4 ;, Ligustrum lucidum 9-10 *, Huai yam 9-10%, Mulberry and Hangbaiju 3 ⁇ ;
  • the auxiliary raw material is a component having the following weight percentages: bran 10-12 ⁇ fine bran 5-63 ⁇ 4, yeast 1-1.2 *, brown sugar 1.5-2 ⁇ 5 *, calcium carbonate 1-1.2 *;
  • the bacterial oxalase is the above-mentioned cellulase of 0.02 * and the above-mentioned amylase of 0.03 ⁇ ;
  • the mixture of basidiomycete puree and / or seaweed puree used for inoculation is: Zhizhi puree, ⁇ Needle mushroom puree, kelp puree.
  • the bacterial oxalase used in the present invention is a permanganase, but the pure hydrolyzed enzymes sold in the present invention are not effective because the enzymes are mainly produced by fungi and it is difficult to effectively treat Chinese herbal medicines. break down.
  • the bacterial oxalase in the present invention is produced by co-fermentation of fungi and group fungi.
  • the above-mentioned cellulase is mainly used, or one of the above-mentioned cellulose is selected from the above-mentioned amylase, pectinase, and protease. Enzymes are used together.
  • the basidiomycetes, algae, and Chinese herbal medicines of the present invention are all dry products with impurities removed, and the weight of each raw material is weighed according to the formula. ⁇ , ' ⁇ ',
  • the above fungi and algae are pulverized and ground into powder, respectively, and the Chinese herbal medicine is pulverized into a 16-20 mesh fineness powder.
  • the enzyme promoter, ammonia source, carbon source, and nutritional additive of each auxiliary original product are further mixed, and then mixed and stirred evenly.
  • the present invention is produced by a liquid deep aeration fermentation method.
  • the above-mentioned fully-mixed raw soup is first placed in a bioreactor, the fermentation temperature is 30 "C-5 5 V, and the p H value is adjusted to 4.0-6 with lactic acid. 5, fermentation time 5-8 days, stirring 1 "2 times a day. 24 hours from the start of fermentation, a mixture of 1-2% of the basidiomycete puree and / or seaweed puree is added at intervals of 24 hours.
  • the fermented mixture was placed in an ultracentrifuge, and the temperature of the rotor was controlled at zero. -5 ⁇ (: between, centrifugation time 10 minutes / times, take the clear wave, centrifuge the slag plus lactic acid to extract for 24 hours and ultracentrifuge again to get the clear wave, combine the original supernatant after the second supernatant centrifugation Dregs can be used as feed additives.
  • the two obtained supernatants were adsorbed with a pore size 18 ⁇ -2 A activated carbon, and unsaturated fatty acids were removed and decolorized.
  • the decolorized supernatant water is concentrated by a dehydrator to a gelatinous body with a combined water content of 30-35%.
  • the finished powder product is vacuum packed into bottles at 10 mg / bottle.
  • the fermentation time is 72 hours, the temperature is 37 ⁇ l e C, and the pH value is 6.0-7.0.
  • the culture product contains 30% of the dried amylase.
  • basidiomycetes and seaweed 440 g of Lentinus edodes, 110 g of Flammulina velutipes, 110 g of Poria cocos, 20 g of Ganoderma lucidum, 2 2 O.g of kelp;
  • Chinese herbal medicines Bo Ziren 230 g, Snake Bed 110 g, Cuscuta 110 g., Raspberry 110 g, Dendrobium 75 g, Morinda officinalis 45 g, Eucommia 90 g, Asparagus 9 0 g, Polygala spp.
  • the fermented mixture was centrifuged in an ultra-centrifuge centrifuge at 2 'C for 10 minutes, and the clear water was taken. The centrifuged residue was leached with lactic acid for 24 hours. The centrifuge was centrifuged for 10 minutes and combined twice. The clear solution is adsorbed with activated carbon with a pore size of 18 A to remove the unsaturated medium fat. After the fat is decolorized, it is concentrated by a dehydrator to a colloid with a water content of 35%, and then placed in a temperature-controlled drying box at about 60 C. Dry for 4 hours to make 2 kg of powdered finished product with a total volume of 6%.
  • basidiomycetes and seaweed 70 g of Lentinus edodes, 60 g of Flammulina velutipes, 10 g of Poria cocos, 30 g of Ganoderma lucidum, and 70 g of kelp;
  • bran 70 g of bran, 80 g of fine bran, 40 g of sweet potato powder, 12 g of yeast, sucrose
  • the mixture used to inoculate the basidiomycete puree and / or seaweed puree is: 0.16 g puree of mushrooms, 0.08 g of puree of mushrooms, ganoderma and kelp (1: 1) puree 0. 0 9 g.
  • Example 1 The method of Example 1 was repeated to produce 300 g of a powder finished product with a water content of 6%.
  • basidiomycetes and seaweeds 70 g of Poria cocos, 80 g of Ganoderma lucidum and 70 g of kelp;
  • Chinese herbal medicine Polygonum multiflorum (manufactured) 50 g, Austrodia chinensis 40 g, Cuscuta chinensis 40 g, Ligustrum lucidum 40 g, Eucommia ulmoides 40 g, Agaricus syringa 60 g, Mulberry leaf 20 g, 40 g of silver flower, 40 g of black mulberry, 4 g of wolfberry, 60 g of chrysanthemum, 40 g of rehmannia glutinosa, 30 g of yam meat, 40 g of tannin, 4 g of diarrhea, and 40 g of yam;
  • the mixture of basidiomycete puree and / or seaweed puree used for inoculation is: 0.16 g of mushroom puree, 0-16 g of mushroom puree, and 0 ⁇ 0 9 g of kelp puree.
  • Example 1 The method of Example 1 was repeated to prepare a powder finished product with a water content of 6 9 & 9 4 0 g.
  • This product can calm blood and nourish yin, dissipate blood stasis and clear collaterals, and lower blood lipids.
  • bran 45 g of bran, 45 g of fine bran, 15 g of yeast, 35 g of brown sugar, 3'5 g of soy flour, 10 g of calcium carbonate ;
  • the mixture of basidiomycete puree and / or seaweed puree used for inoculation is: 0.2 g of mushroom puree, kelp puree 0.1 g, ganoderma puree 0,, 1 g ?.
  • Example 1 The method of Example 1 was repeated to produce a finished product of 6% of the total volume of 11.8 g.
  • the mixture of basidiomycete puree and / or seaweed puree used for inoculation is: lenticular puree 0.16 g, kelp puree 0. 8 g, and ganoderma puree 0. 9 g.
  • Example 1 The method of Example 1 was repeated to produce a finished product 715 g with a water content of 6%.
  • Alisma 40 g Hawthorn 40 g, Yin Chen 40 g, Buffalo Horn 30 g, Epimedium 30 g and Rhubarb 6 g;
  • auxiliary raw mash 100 g of bran, 60 g of fine bran, 60 g of sweet potato flour, 12 g of baker's yeast, 12 g of brown sugar, and 12 g of calcium carbonate;
  • the mixture of basidiomycete puree and / or seaweed puree used for inoculation is: 0.15 g of mushroom puree, 0.08 g of mushroom puree, and 0.99 g of ganoderma puree.
  • Example 1 The method of Example 1 was repeated to obtain a finished product of 6 8 5 g with a hydration content of 6 %.
  • Chinese herbal medicine Polygonum multiflorum 220 g, Ligustrum lucidum 100 g, Huai yam 100 g, Mulberry 50 g and Hangbai chrysanthemum 40 g;
  • auxiliary materials 120 g of bran, 60 g of fine bran, 12 g of yeast, 20 g of brown sugar, 12 g of calcium carbonate;
  • Bacterial oxidase was used as reference ⁇ vitamin E enzyme 2 ⁇ 2 g made in Reference Example 3 -B and ⁇ amylase 0.1 g made in Reference Example 1;
  • This product has the functions of nourishing blood, nourishing liver, nourishing sage, and promoting hair growth. It is used for baldness, alopecia areata, alopecia areata, and young white hair.
  • the invention has the following characteristics, and thus can have a wide range of applications:
  • the persuasive health care medicine prepared by the method of the present invention is combined with bacterial protein, seaweed single-cell soluble protein, active ingredients of Chinese herbal medicines, and trace nutrients.
  • Nutrient elements It is composed of small molecule proteins with strong cell activity. And peptide substances, can penetrate the brain barrier and cell membranes, reach diseased areas, and kill disease factors and viruses. Clinically proven, this drug has a significant effect on the treatment of diseases such as aging and aging, and can delay the aging effect.
  • the persuasion, health-preserving medicine of the present invention has the obvious persuasion effect that the curative effect is good and the effect is fast compared with the similar formula and the medicine made by other processing methods.
  • the total amount (weight percentage) of various components measured in the health medicine prepared by the method of the present invention is: protein 10.0463 ⁇ 4 fat 2.843 *
  • Iron 150 Vitamin (mg / 100g)
  • Amino acid germanium Oppm / ipog,. ⁇ .
  • Example 1 20 mice were used for experiments; the females were half prepared, and the highest concentration (28%) of the biological fermentation product of the present invention was 0.4ml / 10gBW. Once every 4 hours, 3 times in a row. Toxicity and death were observed at the end of 7 days.
  • LD 5 was calculated by intragastric administration of the biologically fermented mice of the present invention. More than 33.6g / kg, equivalent to 917 times the recommended clinical dosage. ''
  • Example 5 For all kinds of diseases caused by visceral deficiency and yin-yang tone; It has 62 cases of sexually transmitted diseases and senile diseases. It is clinically applied in Example 2.
  • the products are diagnosed according to the "Specifications for the Diagnosis and Treatment of Traditional Chinese Medical Diseases". The standard indicates that: the total effective rate for heart-yang deficiency (total 21 cases) is 95%; the total effective rate for heart-spleen deficiency (total 20 cases) is 90%; 1 case) with a total effective rate of 90; the total effective rate with respect to life-gate fire decay (a total of 10 cases) was 92%.
  • the preparation process of the present invention is simple and easy, the equipment is simple, the original tin is easy to obtain, the cost is low, and it is convenient for popularization and application.

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  • Medicines Containing Plant Substances (AREA)

Abstract

Médicament tonique à base de basidiomycètes, d'algues, d'herbes officinales chinoises et d'hydrolases, obtenu au moyen d'un procédé de fermentation biologique, et procédé correspondant. Le médicament comporte de 32 à 48 % en poids de basidiomycètes et d'algues, et de 52 à 68 % en poids d'herbes officinales chinoises. Ledit médicament contient des quantités importantes de polypeptides et de protéines à activité moléculaire de faibles dimensions, il est enrichi en matières nutritives, et il permet de protéger efficacement la santé, de lutter contre la dégénérescence sénile et de traiter certaines pathologies difficiles et compliquées.
PCT/CN1994/000070 1994-01-08 1994-09-02 Medicament tonique a base de basidiomycetes, d'algues et d'herbes officinales chinoises, et son procede de preparation WO1995018626A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU75300/94A AU7530094A (en) 1994-01-08 1994-09-02 A tonic drug made from basidiomycetes, algae, chinese herbal medicine and the method thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN94100345.0 1994-01-08
CN94100345A CN1063331C (zh) 1994-01-08 1994-01-08 一种以菌类、海藻类、中草药为主要原料制备的保健药物

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WO1995018626A1 true WO1995018626A1 (fr) 1995-07-13

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PCT/CN1994/000070 WO1995018626A1 (fr) 1994-01-08 1994-09-02 Medicament tonique a base de basidiomycetes, d'algues et d'herbes officinales chinoises, et son procede de preparation

Country Status (3)

Country Link
CN (1) CN1063331C (fr)
AU (1) AU7530094A (fr)
WO (1) WO1995018626A1 (fr)

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KR100820291B1 (ko) 2006-11-10 2008-04-07 제주대학교 산학협력단 발효된 해조류의 상등액을 유효성분으로 포함하는 혈액응고억제용 조성물 및 그 제조방법
CN102321598A (zh) * 2011-09-05 2012-01-18 浙江大学 一种酵母展示型果胶酶协同超声波破碎紫菜细胞的方法
CN105567665A (zh) * 2016-03-25 2016-05-11 深圳市先康达生物科技有限公司 一种高效角蛋白酶生产方法
CN112753916A (zh) * 2020-12-31 2021-05-07 厦门市天德善品生物科技有限公司 女贞子明目饮品及其制备方法
CN112772914A (zh) * 2020-12-31 2021-05-11 厦门市天德善品生物科技有限公司 虫草人参蕈草营养素及其制备方法

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CN101361571B (zh) * 2008-10-10 2012-09-26 李绩 补肾壮阳食用菌滋补汤产品及其生产方法
CN102488253B (zh) * 2011-12-28 2013-01-30 青岛明月海洋科技有限公司 一种活性海藻提取物的制备方法
CN103689353B (zh) * 2013-12-06 2016-03-23 安徽汇阳食品有限公司 一种补血益气保健粉及其制备方法
CN104322917A (zh) * 2014-09-26 2015-02-04 安徽省巴克夏种猪育种有限责任公司 一种含食用菌渣发酵猪饲料及其制备方法
CN111281964A (zh) * 2019-12-04 2020-06-16 红华御康生物科技(江苏)股份有限公司 一种中药发酵活性多肽口服液及其应用
CN112890186A (zh) * 2021-02-03 2021-06-04 杭州世子合德生物科技有限公司 一种酶类养生保健品提取方法

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CN1062089A (zh) * 1990-11-30 1992-06-24 张文明 一种食用真菌制备天然饮料的工艺
CN1077638A (zh) * 1992-04-14 1993-10-27 郝超焰 一种孕妇口服营养药的制法
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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100820291B1 (ko) 2006-11-10 2008-04-07 제주대학교 산학협력단 발효된 해조류의 상등액을 유효성분으로 포함하는 혈액응고억제용 조성물 및 그 제조방법
CN102321598A (zh) * 2011-09-05 2012-01-18 浙江大学 一种酵母展示型果胶酶协同超声波破碎紫菜细胞的方法
CN105567665A (zh) * 2016-03-25 2016-05-11 深圳市先康达生物科技有限公司 一种高效角蛋白酶生产方法
CN112753916A (zh) * 2020-12-31 2021-05-07 厦门市天德善品生物科技有限公司 女贞子明目饮品及其制备方法
CN112772914A (zh) * 2020-12-31 2021-05-11 厦门市天德善品生物科技有限公司 虫草人参蕈草营养素及其制备方法

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CN1104902A (zh) 1995-07-12
CN1063331C (zh) 2001-03-21
AU7530094A (en) 1995-08-01

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