CN108419970A - A kind of chu chrysanthemum Periostracum cicadae fermented beverage and preparation method thereof - Google Patents
A kind of chu chrysanthemum Periostracum cicadae fermented beverage and preparation method thereof Download PDFInfo
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- CN108419970A CN108419970A CN201810085936.7A CN201810085936A CN108419970A CN 108419970 A CN108419970 A CN 108419970A CN 201810085936 A CN201810085936 A CN 201810085936A CN 108419970 A CN108419970 A CN 108419970A
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- periostracum cicadae
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
- A23L2/382—Other non-alcoholic beverages fermented
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
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Abstract
The present invention relates to field of health care food, and in particular to a kind of chu chrysanthemum Periostracum cicadae fermented beverage and preparation method thereof, preparation method includes the following steps：（1）The activation culture of Periostracum cicadae strain is carried out on culture medium；（2）The liquid fermentation and culture of Periostracum cicadae strain；（3）It prepares and contains chu chrysanthemum Periostracum cicadae fluid nutrient medium, fermented and cultured Periostracum cicadae；（4）Other dispensings, mixing are added into the chu chrysanthemum Periostracum cicadae zymotic fluid fermented；（5）Filling, sterilizing, degassing, capping.Preparation method of the invention is easy to operate, the period is short；Chu chrysanthemum Periostracum cicadae fermented beverage obtained has the smell of fragrance, without the distinctive bitter taste of chu chrysanthemum, and it is full of nutrition, have improve the immunity of the human body, strengthening by means of tonics, nourish the liver to improve visual acuity, anti-aging, the health-care efficacies such as antiviral.The diversity of cicada fungus and chu chrysanthemum product is enriched simultaneously.In addition, implementation condition of the present invention is easy to reach, extensive industry popularization can be carried out.
Description
Technical field
The present invention relates to a kind of processing of nutrient health-care beverage, specifically a kind of chu chrysanthemum Periostracum cicadae fermented beverage and its preparation
Method belongs to field of health care food.
Background technology
Periostracum cicadae, also referred to as golden cicada spend (Cordyceps cicadae), and cicada grass, cicada are also referred to as in China different regions
Young pilose antler, worm flower, Hu cicada etc.；Cicada larva is colonized in for cicada Isaria and is formed by entomogenous fungi complex, is China's tradition rare traditional Chinese medicine.
Its is cold in nature, sweet in flavor, nontoxic, can dispelling wind and heat from the body, arresting convulsion antispastic, improving eyesight promoting eruption, strengthening by means of tonics.Early in the Northern and Southern Dynasties in fiveth century of Christian era
Period, that is, on the books, thunder Xiao exist《Lei Gong Pao Zhi Lun》The processing method for describing cicada fungus：" it is all to make, the full person of white flower, receive after in
Southeast corner is outstanding dry under room, after removing soil, boil one to night with pulp-water, dry fine ground with it ", later, Sui, Tang Zhenquan《Pharmacological property
By》, Song Dynasty Su Song《Figure is through book on Chinese herbal medicine》, Ming Dynasty's Li Shizhen《Compendium of Materia Medica》It is on the books.Over over thousands of year, cicada fungus is applied always
In keeping fit and healthy, disease is treated in diseases prevention.Yunnan Province of China, Guangdong, Sichuan, Fujian and Zhejiang, Jiangsu one are accustomed to long-term consumption, have opened
Numerous cicada fungus medicated nourishing recipes are sent out, is suitble to different constitutions, the people of illness and healthy population to the ill or body-building is selected.Cicada fungus
Cordyceps sinensis has edible region and long edible history extensively in China.
Research in recent years shows no matter natural cicada fungus or the cicada fungus manually cultivated are rich in protein, amino acid (including 8
Amino acid kind needed by human), cellulose, aliphatic acid, multivitamin, the nutriments such as trace element and carbohydrate.Artificial training
Foster cicada fungus, there are identical nutritional ingredients with natural cicada fungus, and fragrance is agreeable to the taste, have to natural cicada fungus and substitute well
Property.Cicada fungus simultaneously can generate a variety of physiological activators to play an important role in health care, including polysaccharide, cyclic peptide, core
Glycoside, sterols, shell rhzomorph etc..Kiho and Nagai isolates galactomannans C-3 from cicada fungus
(galactomannan), it is a kind of water-soluble polysaccharide, relative molecular weight 27000, by D-MANNOSE and D- galactolipins with 4:3
Ratio forms, and with the dosage of 20mg/kg to the antitumor action of mouse S 180 sarcoma, as a result inhibiting rate is 47%.Duarte N
Deng detaching to obtain five kinds of cyclic peptide compounds, respectively muscardine ketone (Bassiatin), muscardine ketone first (Bassiatin from cicada fungus
A), beauvericin (Beauvericin), beauvericin first (Beauvericin A), beauvericin second (Beauvericin B).
Muscardine ketone and muscardine ketone first have similar pharmacological action, are a kind of novel platelet aggregation inhibitor, lure ADP
The rabbit platelet cohesion led has apparent inhibiting effect (IC50=1.9 × 10-4mol/L).The pharmacological results show muscardine
Plain constituents have the effects that antitumor, anticonvulsion, anti-arrhythmia, calmness.Cicada fungus Nucleosides include adenosine, cordyceps sinensis
Element, guanosine, uridine, adenine, guanine etc..Adenosine is a kind of purine nucleosides, and content is the various cordyceps sinensis inherent qualities of evaluation
Leading indicator.Adenosine is related to the adjusting of different physiology courses in central nervous system, can inhibit the excitement of axoneuron
Property, can expand coronal and peripheral vessels, increase coronary blood flow, reduce blood pressure, reducing heart rate the effects that, adenosine also has
Have platelet aggregation-against, radioresistance and it is antitumor the effects that.Kuo and Lin isolates ergosterol from CSM treated body
(Ergosterol) and ergosterol peroxide (Ergosterol peroxide).Ergosterol is on Mycophyta cell membrane
Important steroid, content is stablized relatively in cordyceps sinensis class fungi, usually as one of quality control index, has anti-oxidant energy
Power, and be the predecessor of calciferol, there is important pharmacological action.Kuo and Weng report ergosterol peroxide has
It is living to can inhibit plant hemagglutinin (PHA) for the effect for inhibiting tumour growth and anti-inflammatory activity and antiatherosclerosis
Human T-cell's differentiation of change and proliferation.
Takano and Yahagi isolates a kind of proteoglycan that can promote Th1 immune responses from cicada fungus zymotic fluid,
The polysaccharide can increase the concentration of proleulzin and interferon-γ in rat Pai Shi aggregated lymphoid nodal cells, and pass through toxicity
Experiment shows that the proteoglycan does not have toxic side effect to rat.Zhu Rong et al. researchs find that Periostracum cicadae extract can have
Effect reduces the renal fibrosis process of experimental model mouse.Wang Hualin et al. have found cicada fungus worm by proteomics research
Careless water extract can slow down the increment of liver cancer cells MHCC97H cells.Weng SC et al. researchs find Periostracum cicadae extract also
With immunoregulatory effect.
Current wild Periostracum cicadae is mainly Baoshang as food materials primarily as Chinese medicine or as food materials.But by
In wild Periostracum cicadae limited amount, and wild Periostracum cicadae quality is irregular, and heavy metal easily occurs in especially wild cicada fungus
Exceeded phenomenon.Meanwhile acquisition wild-growing ginseng can also cause natural environment certain destruction.In recent years, Periostracum cicadae is manually cultivated
Technology is rapidly developed.But artificial Periostracum cicadae is mainly with the powder or cicada fungus soaking wine after crushing at present
Form is sold, and the form of product is relatively simple, and is not conducive to good health it is known that drinking.
Chu chrysanthemum is national geography famous special product, is the specialty of Chuzhou, mainly originates in Anhui Province's Chuzhou City, sweet in flavor, cold nature,
Because having good medicinal efficacy, it is known as first of China's " four big medicine chrysanthemums ", being commonly called as feverfew, camomile is that the Ministry of Public Health clearly ratifies
Can be with the food of medicine-food two-purpose, and had more than 2000 years plantation history away from the present.Modern study, which has, to be shown in chu chrysanthemum
Containing natural constituents such as flavonoids, essential oil, polysaccharide and polyphenols, there is antibacterial, antipyretic, decompression, anti-aging, disease-resistant
The multiple functions such as poison, immune, the improving eyesight flat liver removing toxic substances of enhancing.But the major product of chu chrysanthemum is still to be as Herb Tea to dry petal
Main, product form is also very poor.And chu chrysanthemum brewed for drinking has certain bitter taste, causes many people not like and drinks.
Invention content
The present invention provides a kind of preparation method of Periostracum cicadae fermented beverage, it is therefore an objective to horn of plenty beverage market and cicada fungus worm
Grass product form and a kind of fermentation Periostracum cicadae composite beverage full of nutrition, with a variety of physiological regulation functions is provided.
The present invention is achieved by the following technical solutions：
A kind of chu chrysanthemum Periostracum cicadae fermented beverage is made of chu chrysanthemum, Periostracum cicadae and other dispensings through liquid fermentation.
Preferably, including the following raw material：Periostracum cicadae spore suspension 1 × 105-6A/mL, 2.5~3.5wt% of chu chrysanthemum,
0.6~1.4wt of edible glue ‰, 3~5wt of sucrose ‰, 0.05~0.15wt of citric acid ‰, 0.02~0.04wt of vitamin C ‰, color
Element 0~0.02wt ‰.
Preferably, the edible glue be gellan gum or be guar gum and xanthans mixture.
Further, the additive amount of the gellan gum is 0.6~0.9wt ‰.
Further, the additive amount of the guar gum and xanthans is 1.2~1.4wt ‰, and the weight ratio of the two
It is 1:1.
Another object of the present invention is to provide the preparation methods of the chu chrysanthemum Periostracum cicadae fermented beverage, including following step
Suddenly：
Step 1. actication of culture
The potato 200g of clean peeling is thinly sliced, a certain amount of boiling boiling half an hour is added, with 6-8 layers of crocus cloth
Potato residue is filtered off, sucrose 20g, agar 20g is added, water consumption supplies 1000mL, and heating was boiled within 5 minutes, waited for agar
It dissolves, is dispensed into test tube rapidly while hot completely, each cuvette cartridge 5-7mL starts to sterilize after stoppering sealing with silica gel plug, high pressure
Pressurize takes the dish out of the pot for 30 minutes when sterilizing pot temperature reaches 121 DEG C -123 DEG C, test tube is put into inclined-plane while hot, culture medium must not contact silicon
Rubber plug, cooling back bevel is at solid-like.By the Periostracum cicadae strain preserved in refrigerator (cicada Isaria CUIC0001, Isaria
Cicadae CUIC0001, the strain on December 14th, 2015 in China typical culture collection center preservation, compile by preservation
Number be CCTCC NO.2015745, preservation address be the Wuhan Wuhan Universitys of China) be forwarded on the slant medium newly prepared,
Then test tube is put into 20-25 DEG C of constant incubator and is cultivated 15-20 days or so, until can be spare after big volume production spore.
Step 2. Cordyceps cicadae strain culture
The potato 200g of clean peeling is thinly sliced, a certain amount of boiling boiling half an hour is added, horse is filtered off with crocus cloth
Bell potato residue is added sucrose 20g, potassium dihydrogen phosphate 0.3g, magnesium sulfate 0.15g, selenate 20mg, water consumption and supplies 1000mL,
5 minutes mixings are boiled in heating, are sub-packed in triangular flask 500mL, per bottled 200-250mL.It is sealed with silica gel plug, is placed in autoclave
Middle sterilizing.Pressurize 20 minutes when temperature reaches 121 DEG C -123 DEG C are put into superclean bench after cooling, together with other inoculation articles for use
Apparatus irradiates ultraviolet light 30 minutes together, prepares inoculation.The effect for adding potassium dihydrogen phosphate, magnesium sulfate is since cicada fungus is in liquid
During culture, pH value can lower, and two kinds of salt of addition can buffer the pH value variation of culture solution first, followed by be given birth to strain
It is long that mineral element is provided.
Seeded process will strictly observe sterile working regulation, thus inoculation article and implement and operating personnel have to pass through it is sterile
It can be operated after change processing.Seeded process is will to produce the good Periostracum cicadae slant tube strain of spore, and injection 10mL goes out
The distilled water of bacterium pours into 50mL triangular flasks and is configured to spore suspension after scraping spore with oese.Each 500mL triangular flasks connect
Kind 5-10mL spore suspensions.When Liquid Culture base unit weight increases, inoculum concentration analogizes increase according to the above ratio.The triangle that will be inoculated with
Bottle is positioned in constant-temperature shaking incubator, is placed in 130-160r/min, the shaken cultivation case culture 36-56 under the conditions of 25 DEG C is small
When.
Step 3. seeding tank fermented and cultured
20L fluid nutrient mediums and edible defoaming agent are packed into 30L airlift fermentors, at 121 DEG C, 0.11MPa conditions
Lower pressurize sterilizes 30 minutes；After sterilizing, when being cooled to 20-25 DEG C, by 1 bottle of above-mentioned cultured 500mL shaking flasks strain in flame
Protection, which is sowed, to be connected in fermentation tank, is 25 ± 1 DEG C in cultivation temperature, ventilatory capacity is cultivated 36-48 hours under conditions of being 1.5vvm.
Step 4. ferment tank culture
It is packed into 2/3 fluid nutrient medium and edible defoaming agent in 300-500L airlift fermentors, prepares the Liquid Culture
The 2.5-3.5% chu chrysanthemum chrysanthemum hot water extraction liquid that base uses, i.e. the chu chrysanthemum chrysanthemum leaching that every liter of 80-95 DEG C of hot water adds 25-35g selected
Bubble 5-10 minutes prepares liquid fermentation medium with it according to the formula in step 2.At 121 DEG C, pressurize under the conditions of 0.11Mpa
Sterilizing 30-45 minutes；After sterilizing, when being cooled to 20-25 DEG C, pass through strain has been cultivated in seed fermentation tank with the culture transferring of sterilizing
Pipeline is transferred in fermentation tank, and cultivation temperature is 25 ± 1 DEG C, ventilatory capacity 1.5vvm, is cultivated, is reached through ventilation in 36-48 hours
It is spare after exponential phase.
Step 5. adds gellan gum powder 0.6-0.9g or Guar into the Periostracum cicadae zymotic fluid fermented according to 1000mL
Bean gum powder and xanthan rubber powder press (1：1) 1.2-1.4g, sucrose 3-5g, citric acid 0.05-0.15g, vitamin C 0.02-0.04g,
Mixing.
Step 6. is filling
The prepared beverage of step 5 is dispensed into Packaging Bottle.
Step 7. sterilizes
Canned beverage is sterilized 15-30 minutes at 120-125 DEG C, pressure 0.11MPa.
Step 8. deaerates
The air in beverage is pumped with vacuum degassing machine, is aseptically carried out.
Step 9. covers
The Packaging Bottle after degassing is sealed with capsuling machine, is aseptically carried out.
The beneficial effects of the present invention are：
Chu chrysanthemum is prepared fermented beverage by the present invention by Periostracum cicadae liquid fermentation, has expanded chu chrysanthemum, Periostracum cicadae product
Form；Peculiar pleasant fragrant is presented in bitter taste when gained fermented beverage is individually made tea without chu chrysanthemum；And full of nutrition, tool
It is improved body immunity, strengthening by means of tonics, nourishes the liver to improve visual acuity, anti-aging, the health-care efficacies such as antiviral.It is also fully utilized by cicada simultaneously
Zymotic fluid during pink bollworm grass liquid fermentation, reduces environmental pollution.Preparation method is easy to operate, the period is short；In addition, this hair
Bright implementation condition is easy to reach, and can carry out extensive industry popularization.
Specific implementation mode
To be best understood from the present invention, with reference to embodiment, the invention will be further described, and following embodiment is only pair
The present invention is illustrated rather than is limited to it.
Embodiment 1 has the preparation of bacterium ball chu chrysanthemum Periostracum cicadae fermented beverage
Step 1. actication of culture
The potato 200g of clean peeling is thinly sliced, a certain amount of boiling boiling half an hour is added, with 6-8 layers of crocus cloth
Potato residue is filtered off, sucrose 20g, agar 20g is added, water consumption supplies 1000mL, and heating was boiled within 5 minutes, waited for agar
It dissolves, is dispensed into test tube rapidly while hot completely, each cuvette cartridge 5-7mL starts to sterilize after stoppering sealing with silica gel plug, high pressure
Pressurize takes the dish out of the pot for 30 minutes when sterilizing pot temperature reaches 121 DEG C, and test tube is put into inclined-plane while hot, and culture medium must not contact silica gel plug,
Cooling back bevel is at solid-like.By on the Periostracum cicadae strain transfer to the slant medium newly prepared that refrigerator preserves, then
Test tube is put into 25 DEG C of constant incubator and is cultivated 15 days or so, until can be spare after big volume production spore.
Step 2. Cordyceps cicadae strain culture
The potato 200g of clean peeling is thinly sliced, a certain amount of boiling boiling half an hour is added, horse is filtered off with crocus cloth
Bell potato residue is added sucrose 20g, potassium dihydrogen phosphate 0.3g, magnesium sulfate 0.15g, selenate 20mg, water consumption and supplies 1000mL,
5 minutes mixings are boiled in heating, are sub-packed in 500mL triangular flasks, per bottled 200-250mL.It is sealed with silica gel plug, is placed in autoclave
Middle sterilizing.Pressurize 20 minutes when temperature reaches 121 DEG C are put into superclean bench after cooling, together with other inoculation article and implements one
It plays irradiation ultraviolet light 30 minutes, prepares inoculation.The effect for adding potassium dihydrogen phosphate, magnesium sulfate is since cicada fungus is in Liquid Culture
In the process, pH value can lower, and two kinds of salt of addition can buffer the pH value variation of culture solution first, followed by be provided to growth
Mineral element.
Seeded process will strictly observe sterile working regulation, thus inoculation article and implement and operating personnel have to pass through it is sterile
It can be operated after change processing.Seeded process is will to produce the good Periostracum cicadae slant tube strain of spore, and injection 10mL goes out
The distilled water of bacterium pours into 50mL triangular flasks and is configured to spore suspension after scraping spore with oese.Each 500mL triangular flasks connect
Kind 5mL spore suspensions.When Liquid Culture base unit weight increases, inoculum concentration analogizes increase according to the above ratio.The triangular flask that will be inoculated with
It is positioned in constant-temperature shaking incubator, is placed in 130r/min, the shaken cultivation case culture under the conditions of 25 DEG C 36 hours.
Step 3. seeding tank fermented and cultured
20L fluid nutrient mediums and edible defoaming agent are packed into 30L airlift fermentors, at 121 DEG C, 0.11MPa conditions
Lower pressurize sterilizes 30 minutes；After sterilizing, when being cooled to 25 DEG C, 1 bottle of above-mentioned cultured 500mL shaking flasks strain is protected in flame
It sows and is connected in fermentation tank, be 25 ± 1 DEG C in cultivation temperature, ventilatory capacity is cultivated 36 hours under conditions of being 1.5vvm.
Step 4. ferment tank culture
2/3 fluid nutrient medium and edible defoaming agent are packed into 300L airlift fermentors, preparing the fluid nutrient medium makes
3% chu chrysanthemum chrysanthemum hot water extraction liquid, i.e. the chu chrysanthemum chrysanthemum that every liter of 80-95 DEG C of hot water adds 30g selected impregnate 10 minutes, use it
Liquid fermentation medium is prepared according to the formula in step 2.At 121 DEG C, pressurize sterilizing 30 minutes under the conditions of 0.11Mpa；Sterilizing
Afterwards, when being cooled to 25 DEG C, strain will have been cultivated in seed fermentation tank by being transferred in fermentation tank with the culture transferring pipeline of sterilizing, has been trained
It is 25 ± 1 DEG C, ventilatory capacity 1.5vvm to support temperature, is cultivated through ventilation in 48 hours, reaches spare after exponential phase.
Step 5. is into the Periostracum cicadae zymotic fluid fermented according to 1000mL addition gellan gum powder 0.7g, sucrose 5g, lemon
Lemon acid 0.1g, vitamin C 0.02g, appropriate natural pigment, mixing.
Step 6. is filling
The prepared beverage of step 5 is dispensed into Packaging Bottle.
Step 7. sterilizes
Canned beverage is sterilized 20 minutes at 121 DEG C, pressure 0.11MPa.
Step 8. deaerates
The air in beverage is pumped with vacuum degassing machine, is aseptically carried out.
Step 9. covers
The Packaging Bottle after degassing is sealed with capsuling machine, is aseptically carried out.
Embodiment 2 has the preparation of bacterium ball chu chrysanthemum Periostracum cicadae fermented beverage
Step 1. actication of culture
The potato 200g of clean peeling is thinly sliced, a certain amount of boiling boiling half an hour is added, with 6-8 layers of crocus cloth
Potato residue is filtered off, sucrose 20g, agar 20g is added, water consumption supplies 1000mL, and heating was boiled within 5 minutes, waited for agar
It dissolves, is dispensed into test tube rapidly while hot completely, each cuvette cartridge 5-7mL starts to sterilize after stoppering sealing with silica gel plug, high pressure
Pressurize takes the dish out of the pot for 30 minutes when sterilizing pot temperature reaches 121 DEG C, and test tube is put into inclined-plane while hot, and culture medium must not contact silica gel plug,
Cooling back bevel is at solid-like.By on the Periostracum cicadae strain transfer to the slant medium newly prepared that refrigerator preserves, then
Test tube is put into 25 DEG C of constant incubator and is cultivated 15 days or so, until can be spare after big volume production spore.
Step 2. Cordyceps cicadae strain culture
The potato 200g of clean peeling is thinly sliced, a certain amount of boiling boiling half an hour is added, horse is filtered off with crocus cloth
Bell potato residue is added sucrose 20g, potassium dihydrogen phosphate 0.3g, magnesium sulfate 0.15g, selenate 20mg, water consumption and supplies 1000mL,
5 minutes mixings are boiled in heating, are sub-packed in 500mL triangular flasks, per bottled 200-250mL.It is sealed with silica gel plug, is placed in autoclave
Middle sterilizing.Pressurize 20 minutes when temperature reaches 121 DEG C are put into superclean bench after cooling, together with other inoculation article and implements one
It plays irradiation ultraviolet light 30 minutes, prepares inoculation.The effect for adding potassium dihydrogen phosphate, magnesium sulfate is since cicada fungus is in Liquid Culture
In the process, pH value can lower, and two kinds of salt of addition can buffer the pH value variation of culture solution first, followed by be provided to growth
Mineral element.
Seeded process will strictly observe sterile working regulation, thus inoculation article and implement and operating personnel have to pass through it is sterile
It can be operated after change processing.Seeded process is will to produce the good Periostracum cicadae slant tube strain of spore, and injection 10mL goes out
The distilled water of bacterium pours into 50mL triangular flasks and is configured to spore suspension after scraping spore with oese.Each 500mL triangular flasks connect
Kind 5mL spore suspensions.When Liquid Culture base unit weight increases, inoculum concentration analogizes increase according to the above ratio.The triangular flask that will be inoculated with
It is positioned in constant-temperature shaking incubator, is placed in 130r/min, the shaken cultivation case culture under the conditions of 25 DEG C 36 hours.
Step 3. seeding tank fermented and cultured
20L fluid nutrient mediums and edible defoaming agent are packed into 30L airlift fermentors, at 121 DEG C, 0.11MPa conditions
Lower pressurize sterilizes 30 minutes；After sterilizing, when being cooled to 25 DEG C, 1 bottle of above-mentioned cultured 500mL shaking flasks strain is protected in flame
It sows and is connected in fermentation tank, be 25 ± 1 DEG C in cultivation temperature, ventilatory capacity is cultivated 36 hours under conditions of being 1.5vvm.
Step 4. ferment tank culture
2/3 fluid nutrient medium and edible defoaming agent are packed into 300L airlift fermentors, preparing the fluid nutrient medium makes
3% chu chrysanthemum chrysanthemum hot water extraction liquid, i.e. the chu chrysanthemum chrysanthemum that every liter of 80-95 DEG C of hot water adds 30g selected impregnate 10 minutes, use it
Liquid fermentation medium is prepared according to the formula in step 2.At 121 DEG C, pressurize sterilizing 30-45 minutes under the conditions of 0.11MPa；It goes out
After bacterium, when being cooled to 25 DEG C, strain will be cultivated in seed fermentation tank by being transferred in fermentation tank with the culture transferring pipeline of sterilizing,
Cultivation temperature is 25 ± 1 DEG C, ventilatory capacity 1.5vvm, is cultivated through ventilation in 48 hours, reaches spare after exponential phase.
Step 5. adds cluster bean rubber powder according to 1000mL into the Periostracum cicadae zymotic fluid fermented and xanthan rubber powder (is pressed
Mass ratio 1：1) 1.3g, sucrose 3g, citric acid 0.08g, vitamin C 0.03g, appropriate natural pigment, mixing.
Step 6. is filling
The prepared beverage of step 5 is dispensed into Packaging Bottle.
Step 7. sterilizes
Canned beverage is sterilized 20 minutes at 121 DEG C, pressure 0.11MPa.
Step 8. deaerates
The air in beverage is pumped with vacuum degassing machine, is aseptically carried out.
Step 9. covers
The Packaging Bottle after degassing is sealed with capsuling machine, is aseptically carried out.
The preparation of 3 sterile ball chu chrysanthemum Periostracum cicadae fermented beverage of embodiment
Step 1. actication of culture
The potato 200g of clean peeling is thinly sliced, a certain amount of boiling boiling half an hour is added, with 6-8 layers of crocus cloth
Potato residue is filtered off, sucrose 20g, agar 20g is added, water consumption supplies 1000mL, and heating was boiled within 5 minutes, waited for agar
It dissolves, is dispensed into test tube rapidly while hot completely, each cuvette cartridge 5-7mL starts to sterilize after stoppering sealing with silica gel plug, high pressure
Pressurize takes the dish out of the pot for 30 minutes when sterilizing pot temperature reaches 121 DEG C, and test tube is put into inclined-plane while hot, and culture medium must not contact silica gel plug,
Cooling back bevel is at solid-like.By on the Periostracum cicadae strain transfer to the slant medium newly prepared that refrigerator preserves, then
Test tube is put into 25 DEG C of constant incubator and is cultivated 15 days or so, until can be spare after big volume production spore.
Step 2. Cordyceps cicadae strain culture
The potato 200g of clean peeling is thinly sliced, a certain amount of boiling boiling half an hour is added, horse is filtered off with crocus cloth
Bell potato residue is added sucrose 20g, potassium dihydrogen phosphate 0.3g, magnesium sulfate 0.15g, selenate 20mg, water consumption and supplies 1000mL,
5 minutes mixings are boiled in heating, are sub-packed in 500mL triangular flasks, per bottled 200-250mL.It is sealed with silica gel plug, is placed in autoclave
Middle sterilizing.Pressurize 20 minutes when temperature reaches 121 DEG C are put into superclean bench after cooling, together with other inoculation article and implements one
It plays irradiation ultraviolet light 30 minutes, prepares inoculation.The effect for adding potassium dihydrogen phosphate, magnesium sulfate is since cicada fungus is in Liquid Culture
In the process, pH value can lower, and two kinds of salt of addition can buffer the pH value variation of culture solution first, followed by be provided to growth
Mineral element.
Seeded process will strictly observe sterile working regulation, thus inoculation article and implement and operating personnel have to pass through it is sterile
It can be operated after change processing.Seeded process is will to produce the good Periostracum cicadae slant tube strain of spore, and injection 10mL goes out
The distilled water of bacterium pours into 50mL triangular flasks and is configured to spore suspension after scraping spore with oese.Each 500mL triangular flasks connect
Kind 5mL spore suspensions.When Liquid Culture base unit weight increases, inoculum concentration analogizes increase according to the above ratio.The triangular flask that will be inoculated with
It is positioned in constant-temperature shaking incubator, is placed in 130r/min, the shaken cultivation case culture under the conditions of 25 DEG C 36 hours.
Step 3. seeding tank fermented and cultured
20L fluid nutrient mediums and edible defoaming agent are packed into 30L airlift fermentors, at 121 DEG C, 0.11MPa conditions
Lower pressurize sterilizes 30 minutes；After sterilizing, when being cooled to 25 DEG C, 1 bottle of above-mentioned cultured 500mL shaking flasks strain is protected in flame
It sows and is connected in fermentation tank, be 25 ± 1 DEG C in cultivation temperature, ventilatory capacity is cultivated 36 hours under conditions of being 1.5vvm.
Step 4. ferment tank culture
2/3 fluid nutrient medium and edible defoaming agent are packed into 300L airlift fermentors, preparing the fluid nutrient medium makes
2.7% chu chrysanthemum chrysanthemum hot water extraction liquid, i.e. the chu chrysanthemum chrysanthemum that every liter of 80-95 DEG C of hot water adds 30g selected impregnate 10 minutes, use
According in step 2 formula prepare liquid fermentation medium.At 121 DEG C, pressurize sterilizing 30-45 minutes under the conditions of 0.11MPa；
After sterilizing, when being cooled to 25 DEG C, strain will have been cultivated in seed fermentation tank by being transferred to fermentation tank with the culture transferring pipeline of sterilizing
In, cultivation temperature is 25 ± 1 DEG C, ventilatory capacity 1.5vvm, is cultivated through ventilation in 48 hours, reaches spare after exponential phase.
Step 5. will ferment Periostracum cicadae karusen and be centrifuged by tube centrifuge 10000r/min, by bacterium ball and mycelia
With separation of fermentative broth, zymotic fluid is obtained.
Step 6. is into the zymotic fluid of centrifugation gained according to 1000mL addition gellan gums 0.7g, sucrose 3g, citric acid
0.08g, vitamin C 0.03g, appropriate natural pigment, mixing.
Step 7. is filling
The prepared beverage of step 6 is dispensed into Packaging Bottle.
Step 8. sterilizes
Canned beverage is sterilized 20 minutes at 121 DEG C, pressure 0.11MPa.
Step 9. deaerates
The air in beverage is pumped with vacuum degassing machine, is aseptically carried out.
Step 10. covers
The Packaging Bottle after degassing is sealed with capsuling machine, is aseptically carried out.
Embodiment 4 has the preparation of bacterium ball chu chrysanthemum Periostracum cicadae fermented beverage
Step 1. actication of culture
The potato 200g of clean peeling is thinly sliced, a certain amount of boiling boiling half an hour is added, with 6-8 layers of crocus cloth
Potato residue is filtered off, sucrose 20g, agar 20g is added, water consumption supplies 1000mL, and heating was boiled within 5 minutes, waited for agar
It dissolves, is dispensed into test tube rapidly while hot completely, each cuvette cartridge 5-7mL starts to sterilize after stoppering sealing with silica gel plug, high pressure
Pressurize takes the dish out of the pot for 30 minutes when sterilizing pot temperature reaches 121 DEG C, and test tube is put into inclined-plane while hot, and culture medium must not contact silica gel plug,
Cooling back bevel is at solid-like.By on the Periostracum cicadae strain transfer to the slant medium newly prepared that refrigerator preserves, then
Test tube is put into 20 DEG C of constant incubator and is cultivated 20 days or so, until can be spare after big volume production spore.
Step 2. Cordyceps cicadae strain culture
The potato 200g of clean peeling is thinly sliced, a certain amount of boiling boiling half an hour is added, horse is filtered off with crocus cloth
Bell potato residue is added sucrose 20g, potassium dihydrogen phosphate 0.3g, magnesium sulfate 0.15g, selenate 20mg, water consumption and supplies 1000mL,
5 minutes mixings are boiled in heating, are sub-packed in 500mL triangular flasks, per bottled 200-250mL.It is sealed with silica gel plug, is placed in autoclave
Middle sterilizing.Pressurize 20 minutes when temperature reaches 122 DEG C are put into superclean bench after cooling, together with other inoculation article and implements one
It plays irradiation ultraviolet light 30 minutes, prepares inoculation.The effect for adding potassium dihydrogen phosphate, magnesium sulfate is since cicada fungus is in Liquid Culture
In the process, pH value can lower, and two kinds of salt of addition can buffer the pH value variation of culture solution first, followed by be provided to growth
Mineral element.
Seeded process will strictly observe sterile working regulation, thus inoculation article and implement and operating personnel have to pass through it is sterile
It can be operated after change processing.Seeded process is will to produce the good Periostracum cicadae slant tube strain of spore, and injection 10mL goes out
The distilled water of bacterium pours into 50mL triangular flasks and is configured to spore suspension after scraping spore with oese.Each 500mL triangular flasks connect
Kind 10mL spore suspensions.When Liquid Culture base unit weight increases, inoculum concentration analogizes increase according to the above ratio.The triangular flask that will be inoculated with
It is positioned in constant-temperature shaking incubator, is placed in 160r/min, the shaken cultivation case culture under the conditions of 25 DEG C 56 hours.
Step 3. seeding tank fermented and cultured
20L fluid nutrient mediums and edible defoaming agent are packed into 30L airlift fermentors, at 121 DEG C, 0.11MPa conditions
Lower pressurize sterilizes 30 minutes；After sterilizing, when being cooled to 20 DEG C, 1 bottle of above-mentioned cultured 500mL shaking flasks strain is protected in flame
It sows and is connected in fermentation tank, be 25 ± 1 DEG C in cultivation temperature, ventilatory capacity is cultivated 48 hours under conditions of being 1.5vvm.
Step 4. ferment tank culture
2/3 fluid nutrient medium and edible defoaming agent are packed into 500L airlift fermentors, preparing the fluid nutrient medium makes
3.5% chu chrysanthemum chrysanthemum hot water extraction liquid, i.e. the chu chrysanthemum chrysanthemum that every liter of 80-95 DEG C of hot water adds 35g selected impregnate 10 minutes, use
According in step 2 formula prepare liquid fermentation medium.At 121 DEG C, pressurize sterilizing 30-45 minutes under the conditions of 0.11MPa；
After sterilizing, when being cooled to 20 DEG C, strain will have been cultivated in seed fermentation tank by being transferred to fermentation tank with the culture transferring pipeline of sterilizing
In, cultivation temperature is 25 ± 1 DEG C, ventilatory capacity 1.5vvm, is cultivated through ventilation in 48 hours, reaches spare after exponential phase.
Step 5. adds cluster bean rubber powder according to 1000mL into the Periostracum cicadae zymotic fluid fermented and xanthan rubber powder (is pressed
Mass ratio 1：1) 1.4g, sucrose 4g, citric acid 0.15g, vitamin C 0.04g, appropriate natural pigment, mixing.
Step 6. is filling
The prepared beverage of step 5 is dispensed into Packaging Bottle.
Step 7. sterilizes
Canned beverage is sterilized 20 minutes at 121 DEG C, pressure 0.11MPa.
Step 8. deaerates
The air in beverage is pumped with vacuum degassing machine, is aseptically carried out.
Step 9. covers
The Packaging Bottle after degassing is sealed with capsuling machine, is aseptically carried out.
The preparation of 5 sterile ball chu chrysanthemum Periostracum cicadae fermented beverage of embodiment
Step 1. actication of culture
The potato 200g of clean peeling is thinly sliced, a certain amount of boiling boiling half an hour is added, with 6-8 layers of crocus cloth
Potato residue is filtered off, sucrose 20g, agar 20g is added, water consumption supplies 1000mL, and heating was boiled within 5 minutes, waited for agar
It dissolves, is dispensed into test tube rapidly while hot completely, each cuvette cartridge 5-7mL starts to sterilize after stoppering sealing with silica gel plug, high pressure
Pressurize takes the dish out of the pot for 30 minutes when sterilizing pot temperature reaches 123 DEG C, and test tube is put into inclined-plane while hot, and culture medium must not contact silica gel plug,
Cooling back bevel is at solid-like.By on the Periostracum cicadae strain transfer to the slant medium newly prepared that refrigerator preserves, then
Test tube is put into 25 DEG C of constant incubator and is cultivated 15 days or so, until can be spare after big volume production spore.
Step 2. Cordyceps cicadae strain culture
The potato 200g of clean peeling is thinly sliced, a certain amount of boiling boiling half an hour is added, horse is filtered off with crocus cloth
Bell potato residue is added sucrose 20g, potassium dihydrogen phosphate 0.3g, magnesium sulfate 0.15g, selenate 20mg, water consumption and supplies 1000mL,
5 minutes mixings are boiled in heating, are sub-packed in 500mL triangular flasks, per bottled 200-250mL.It is sealed with silica gel plug, is placed in autoclave
Middle sterilizing.Pressurize 20 minutes when temperature reaches 123 DEG C are put into superclean bench after cooling, together with other inoculation article and implements one
It plays irradiation ultraviolet light 30 minutes, prepares inoculation.The effect for adding potassium dihydrogen phosphate, magnesium sulfate is since cicada fungus is in Liquid Culture
In the process, pH value can lower, and two kinds of salt of addition can buffer the pH value variation of culture solution first, followed by be provided to growth
Mineral element.
Seeded process will strictly observe sterile working regulation, thus inoculation article and implement and operating personnel have to pass through it is sterile
It can be operated after change processing.Seeded process is will to produce the good Periostracum cicadae slant tube strain of spore, and injection 10mL goes out
The distilled water of bacterium pours into 50mL triangular flasks and is configured to spore suspension after scraping spore with oese.Each 500mL triangular flasks connect
Kind 5mL spore suspensions.When Liquid Culture base unit weight increases, inoculum concentration analogizes increase according to the above ratio.The triangular flask that will be inoculated with
It is positioned in constant-temperature shaking incubator, is placed in 130r/min, the shaken cultivation case culture under the conditions of 25 DEG C 36 hours.
Step 3. seeding tank fermented and cultured
20L fluid nutrient mediums and edible defoaming agent are packed into 30L airlift fermentors, at 121 DEG C, 0.11MPa conditions
Lower pressurize sterilizes 30 minutes；After sterilizing, when being cooled to 25 DEG C, 1 bottle of above-mentioned cultured 500mL shaking flasks strain is protected in flame
It sows and is connected in fermentation tank, be 25 ± 1 DEG C in cultivation temperature, ventilatory capacity is cultivated 36 hours under conditions of being 1.5vvm.
Step 4. ferment tank culture
2/3 fluid nutrient medium and edible defoaming agent are packed into 300L airlift fermentors, preparing the fluid nutrient medium makes
2.5% chu chrysanthemum chrysanthemum hot water extraction liquid, i.e. the chu chrysanthemum chrysanthemum that every liter of 80-95 DEG C of hot water adds 25g selected impregnate 10 minutes, use
According in step 2 formula prepare liquid fermentation medium.At 121 DEG C, pressurize sterilizing 30-45 minutes under the conditions of 0.11MPa；
After sterilizing, when being cooled to 25 DEG C, strain will have been cultivated in seed fermentation tank by being transferred to fermentation tank with the culture transferring pipeline of sterilizing
In, cultivation temperature is 25 ± 1 DEG C, ventilatory capacity 1.5vvm, is cultivated through ventilation in 36 hours, reaches spare after exponential phase.
Step 5. will ferment Periostracum cicadae karusen and be centrifuged by tube centrifuge 10000r/min, by bacterium ball and mycelia
With separation of fermentative broth, zymotic fluid is obtained.
Step 6. is into the zymotic fluid of centrifugation gained according to 1000mL addition gellan gums 0.6g, sucrose 3g, citric acid
0.05g, vitamin C 0.03g, appropriate natural pigment, mixing.
Step 7. is filling
The prepared beverage of step 6 is dispensed into Packaging Bottle.
Step 8. sterilizes
Canned beverage is sterilized 20 minutes at 121 DEG C, pressure 0.11MPa.
Step 9. deaerates
The air in beverage is pumped with vacuum degassing machine, is aseptically carried out.
Step 10. covers
The Packaging Bottle after degassing is sealed with capsuling machine, is aseptically carried out.
The preparation of 6 sterile ball chu chrysanthemum Periostracum cicadae fermented beverage of embodiment
Step 1. actication of culture
The potato 200g of clean peeling is thinly sliced, a certain amount of boiling boiling half an hour is added, with 6-8 layers of crocus cloth
Potato residue is filtered off, sucrose 20g, agar 20g is added, water consumption supplies 1000mL, and heating was boiled within 5 minutes, waited for agar
It dissolves, is dispensed into test tube rapidly while hot completely, each cuvette cartridge 5-7mL starts to sterilize after stoppering sealing with silica gel plug, high pressure
Pressurize takes the dish out of the pot for 30 minutes when sterilizing pot temperature reaches 121 DEG C, and test tube is put into inclined-plane while hot, and culture medium must not contact silica gel plug,
Cooling back bevel is at solid-like.By on the Periostracum cicadae strain transfer to the slant medium newly prepared that refrigerator preserves, then
Test tube is put into 25 DEG C of constant incubator and is cultivated 15 days or so, until can be spare after big volume production spore.
Step 2. Cordyceps cicadae strain culture
The potato 200g of clean peeling is thinly sliced, a certain amount of boiling boiling half an hour is added, horse is filtered off with crocus cloth
Bell potato residue is added sucrose 20g, potassium dihydrogen phosphate 0.3g, magnesium sulfate 0.15g, selenate 20mg, water consumption and supplies 1000mL,
5 minutes mixings are boiled in heating, are sub-packed in 500mL triangular flasks, per bottled 200-250mL.It is sealed with silica gel plug, is placed in autoclave
Middle sterilizing.Pressurize 20 minutes when temperature reaches 121 DEG C are put into superclean bench after cooling, together with other inoculation article and implements one
It plays irradiation ultraviolet light 30 minutes, prepares inoculation.The effect for adding potassium dihydrogen phosphate, magnesium sulfate is since cicada fungus is in Liquid Culture
In the process, pH value can lower, and two kinds of salt of addition can buffer the pH value variation of culture solution first, followed by be provided to growth
Mineral element.
Seeded process will strictly observe sterile working regulation, thus inoculation article and implement and operating personnel have to pass through it is sterile
It can be operated after change processing.Seeded process is will to produce the good Periostracum cicadae slant tube strain of spore, and injection 10mL goes out
The distilled water of bacterium pours into 50mL triangular flasks and is configured to spore suspension after scraping spore with oese.Each 500mL triangular flasks connect
Kind 5mL spore suspensions.When Liquid Culture base unit weight increases, inoculum concentration analogizes increase according to the above ratio.The triangular flask that will be inoculated with
It is positioned in constant-temperature shaking incubator, is placed in 130r/min, the shaken cultivation case culture under the conditions of 25 DEG C 36 hours.
Step 3. seeding tank fermented and cultured
20L fluid nutrient mediums and edible defoaming agent are packed into 30L airlift fermentors, at 121 DEG C, 0.11MPa conditions
Lower pressurize sterilizes 30 minutes；After sterilizing, when being cooled to 25 DEG C, 1 bottle of above-mentioned cultured 500mL shaking flasks strain is protected in flame
It sows and is connected in fermentation tank, be 25 ± 1 DEG C in cultivation temperature, ventilatory capacity is cultivated 36 hours under conditions of being 1.5vvm.
Step 4. ferment tank culture
2/3 fluid nutrient medium and edible defoaming agent are packed into 300L airlift fermentors, preparing the fluid nutrient medium makes
2.5% chu chrysanthemum chrysanthemum hot water extraction liquid, i.e. the chu chrysanthemum chrysanthemum that every liter of 80-95 DEG C of hot water adds 25g selected impregnate 10 minutes, use
According in step 2 formula prepare liquid fermentation medium.At 121 DEG C, pressurize sterilizing 30-45 minutes under the conditions of 0.11MPa；
After sterilizing, when being cooled to 25 DEG C, strain will have been cultivated in seed fermentation tank by being transferred to fermentation tank with the culture transferring pipeline of sterilizing
In, cultivation temperature is 25 ± 1 DEG C, ventilatory capacity 1.5vvm, is cultivated through ventilation in 48 hours, reaches spare after exponential phase.
Step 5. will ferment Periostracum cicadae karusen and be centrifuged by tube centrifuge 10000r/min, by bacterium ball and mycelia
With separation of fermentative broth, zymotic fluid is obtained.
Step 6. is into the zymotic fluid of centrifugation gained according to 1000mL addition gellan gums 0.9g, sucrose 3g, citric acid
0.08g, vitamin C 0.03g, appropriate natural pigment, mixing.
Step 7. is filling
The prepared beverage of step 6 is dispensed into Packaging Bottle.
Step 8. sterilizes
Canned beverage is sterilized 20 minutes at 121 DEG C, pressure 0.11MPa.
Step 9. deaerates
The air in beverage is pumped with vacuum degassing machine, is aseptically carried out.
Step 10. covers
The Packaging Bottle after degassing is sealed with capsuling machine, is aseptically carried out.
Embodiment 7 has the preparation of bacterium ball chu chrysanthemum Periostracum cicadae fermented beverage
Step 1. actication of culture
The potato 200g of clean peeling is thinly sliced, a certain amount of boiling boiling half an hour is added, with 6-8 layers of crocus cloth
Potato residue is filtered off, sucrose 20g, agar 20g is added, water consumption supplies 1000mL, and heating was boiled within 5 minutes, waited for agar
It dissolves, is dispensed into test tube rapidly while hot completely, each cuvette cartridge 5-7mL starts to sterilize after stoppering sealing with silica gel plug, high pressure
Pressurize takes the dish out of the pot for 30 minutes when sterilizing pot temperature reaches 121 DEG C, and test tube is put into inclined-plane while hot, and culture medium must not contact silica gel plug,
Cooling back bevel is at solid-like.By on the Periostracum cicadae strain transfer to the slant medium newly prepared that refrigerator preserves, then
Test tube is put into 25 DEG C of constant incubator and is cultivated 15 days or so, until can be spare after big volume production spore.
Step 2. Cordyceps cicadae strain culture
The potato 200g of clean peeling is thinly sliced, a certain amount of boiling boiling half an hour is added, horse is filtered off with crocus cloth
Bell potato residue is added sucrose 20g, potassium dihydrogen phosphate 0.3g, magnesium sulfate 0.15g, selenate 20mg, water consumption and supplies 1000mL,
5 minutes mixings are boiled in heating, are sub-packed in 500mL triangular flasks, per bottled 200-250mL.It is sealed with silica gel plug, is placed in autoclave
Middle sterilizing.Pressurize 20 minutes when temperature reaches 121 DEG C are put into superclean bench after cooling, together with other inoculation article and implements one
It plays irradiation ultraviolet light 30 minutes, prepares inoculation.The effect for adding potassium dihydrogen phosphate, magnesium sulfate is since cicada fungus is in Liquid Culture
In the process, pH value can lower, and two kinds of salt of addition can buffer the pH value variation of culture solution first, followed by be provided to growth
Mineral element.
Seeded process will strictly observe sterile working regulation, thus inoculation article and implement and operating personnel have to pass through it is sterile
It can be operated after change processing.Seeded process is will to produce the good Periostracum cicadae slant tube strain of spore, and injection 10mL goes out
The distilled water of bacterium pours into 50mL triangular flasks and is configured to spore suspension after scraping spore with oese.Each 500mL triangular flasks connect
Kind 5mL spore suspensions.When Liquid Culture base unit weight increases, inoculum concentration analogizes increase according to the above ratio.The triangular flask that will be inoculated with
It is positioned in constant-temperature shaking incubator, is placed in 130r/min, the shaken cultivation case culture under the conditions of 25 DEG C 36 hours.
Step 3. seeding tank fermented and cultured
20L fluid nutrient mediums and edible defoaming agent are packed into 30L airlift fermentors, at 121 DEG C, 0.11MPa conditions
Lower pressurize sterilizes 30 minutes；After sterilizing, when being cooled to 25 DEG C, 1 bottle of above-mentioned cultured 500mL shaking flasks strain is protected in flame
It sows and is connected in fermentation tank, be 25 ± 1 DEG C in cultivation temperature, ventilatory capacity is cultivated 36 hours under conditions of being 1.5vvm.
Step 4. ferment tank culture
2/3 fluid nutrient medium and edible defoaming agent are packed into 300L airlift fermentors, preparing the fluid nutrient medium makes
3% chu chrysanthemum chrysanthemum hot water extraction liquid, i.e. the chu chrysanthemum chrysanthemum that every liter of 80-95 DEG C of hot water adds 30g selected impregnate 10 minutes, use it
Liquid fermentation medium is prepared according to the formula in step 2.At 121 DEG C, pressurize sterilizing 30-45 minutes under the conditions of 0.11MPa；It goes out
After bacterium, when being cooled to 25 DEG C, strain will be cultivated in seed fermentation tank by being transferred in fermentation tank with the culture transferring pipeline of sterilizing,
Cultivation temperature is 25 ± 1 DEG C, ventilatory capacity 1.5vvm, is cultivated through ventilation in 48 hours, reaches spare after exponential phase.
Step 5. adds cluster bean rubber powder according to 1000mL into the Periostracum cicadae zymotic fluid fermented and xanthan rubber powder (is pressed
Mass ratio 1：1) 1.2g, sucrose 3g, citric acid 0.08g, vitamin C 0.03g, appropriate natural pigment, mixing.
Step 6. is filling
The prepared beverage of step 5 is dispensed into Packaging Bottle.
Step 7. sterilizes
Canned beverage is sterilized 20 minutes at 121 DEG C, pressure 0.11MPa.
Step 8. deaerates
The air in beverage is pumped with vacuum degassing machine, is aseptically carried out.
Step 9. covers
The Packaging Bottle after degassing is sealed with capsuling machine, is aseptically carried out.
The chu chrysanthemum Periostracum cicadae fermented beverage of the preparation of above-described embodiment 1~7 and common chu chrysanthemum tea are subjected to sense organ point respectively
Analysis, analytical standard and result are as shown in the following table 1 and 2：
Table 1. has bacterium ball chu chrysanthemum Periostracum cicadae fermented beverage and common chu chrysanthemum tea organoleptic analysis's table
Note：Different letters are indicated in p<0.01 horizontal upper significant difference.
2. sterile ball chu chrysanthemum Periostracum cicadae fermented beverage of table and common chu chrysanthemum tea organoleptic analysis's table
Note：Different letters are indicated in p<0.01 horizontal upper significant difference.
By the above results as it can be seen that having pole between chu chrysanthemum Periostracum cicadae fermented beverage and common chu chrysanthemum tea prepared by the present invention
Significant difference, chu chrysanthemum leaching liquor significantly improve the flavor and taste of chu chrysanthemum after Periostracum cicadae bacterium is fermented.
Embodiment described above is only that the preferred embodiment of the present invention is described, not to the model of the present invention
It encloses and is defined, under the premise of not departing from design spirit of the present invention, technical side of the those of ordinary skill in the art to the present invention
The various modifications and improvement that case is made should all be fallen into the protection domain of claims of the present invention determination.
Claims (10)
1. a kind of chu chrysanthemum Periostracum cicadae fermented beverage, it is characterised in that：By chu chrysanthemum, Periostracum cicadae and other dispensings through liquid fermentation
It is made.
2. a kind of chu chrysanthemum Periostracum cicadae fermented beverage according to claim 1, which is characterized in that including the following raw material：Cicada
Pink bollworm grass spore suspension 1 × 105-6A/mL, 2.5 ~ 3.5wt% of chu chrysanthemum, 0.6 ~ 1.4 wt ‰ of edible glue, 3 ~ 5 wt ‰ of sucrose,
0.05 ~ 0.15 wt ‰ of citric acid, 0.02 ~ 0.04 wt ‰ of vitamin C, 0 ~ 0.02 wt ‰ of pigment.
3. a kind of chu chrysanthemum Periostracum cicadae fermented beverage according to claim 2, it is characterised in that：The edible glue is that knot is cold
Glue or mixture for guar gum and xanthans.
4. a kind of chu chrysanthemum Periostracum cicadae fermented beverage according to claim 3, it is characterised in that：The addition of the gellan gum
Amount is 0.6 ~ 0.9wt ‰.
5. a kind of chu chrysanthemum Periostracum cicadae fermented beverage according to claim 3, it is characterised in that：The guar gum and Huang
The additive amount of virgin rubber is 1.2 ~ 1.4wt ‰, and the weight ratio of the two is 1:1.
6. a kind of preparation method of claim 1 ~ 5 any one of them chu chrysanthemum Periostracum cicadae fermented beverage, which is characterized in that packet
Include following steps：
（1）The activation culture of Periostracum cicadae strain is carried out on culture medium；
（2）Periostracum cicadae strain after activation is carried out liquid fermentation into culture；
（3）The Periostracum cicadae fermentation medium containing chu chrysanthemum leaching liquor is prepared, the fermentation of chu chrysanthemum Periostracum cicadae beverage is carried out；
（4）Other dispensings, mixing are added into the chu chrysanthemum Periostracum cicadae zymotic fluid fermented；
（5）Filling, sterilizing, degassing, capping.
7. a kind of preparation method of chu chrysanthemum Periostracum cicadae fermented beverage according to claim 6, it is characterised in that：Step
（1）The culture medium is PDA culture medium.
8. a kind of preparation method of chu chrysanthemum Periostracum cicadae fermented beverage according to claim 6, which is characterized in that step
（2）The culture medium prescription of the liquid fermentation and culture is：200g containing potato, sucrose 20g, potassium dihydrogen phosphate in per 1000mL water
0.3g, magnesium sulfate 0.15g and selenate 20mg.
9. a kind of preparation method of chu chrysanthemum Periostracum cicadae fermented beverage according to claim 6, which is characterized in that step
（3）The formula of the Periostracum cicadae fermentation medium containing chu chrysanthemum leaching liquor is：Chu chrysanthemum leaching per 2.5 ~ 3.5wt% of 1000mL
200g containing potato, sucrose 20g, potassium dihydrogen phosphate 0.3g, magnesium sulfate 0.15g and selenate 20mg in extract.
10. a kind of preparation method of chu chrysanthemum Periostracum cicadae fermented beverage according to claim 6, it is characterised in that：It is described
Step（4）Further include centrifugation step before, by bacterium ball and mycelia and separation of fermentative broth, obtains sterile ball zymotic fluid.
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| CN115812950A (en) * | 2022-11-18 | 2023-03-21 | 达州市命之源生物科技有限责任公司 | Cordyceps cicadae enzyme and preparation method thereof |
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