CN105348331A - Extraction method of plant polyphenol - Google Patents

Extraction method of plant polyphenol Download PDF

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Publication number
CN105348331A
CN105348331A CN201510747582.4A CN201510747582A CN105348331A CN 105348331 A CN105348331 A CN 105348331A CN 201510747582 A CN201510747582 A CN 201510747582A CN 105348331 A CN105348331 A CN 105348331A
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Prior art keywords
mangrove bark
polyphenol
mangrove
extracting method
plant polyphenol
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CN201510747582.4A
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肖大年
李武
蒙竹风
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Guangxi University
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Guangxi University
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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07GCOMPOUNDS OF UNKNOWN CONSTITUTION
    • C07G99/00Subject matter not provided for in other groups of this subclass

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The invention discloses an extraction method of plant polyphenol in mango peel. The steps include: 1) chopping and scalding; 2) inactivation of polyphenol oxidase; 3) breaking and homogenizing; 4) ethanol extraction; 5) centrifugation; and 6) extract recovery. The method provided by the invention adopts waste mango peel as the raw material to extract polyphenol, mango peel polyphenol is a water soluble substance, and its water solubility increases along with the rise of temperature. Mango peel contains pectin, polysaccharide, protein and other substances, an ethanol solution of certain concentration has certain blocking effect on the hydrogen bond and hydrophobic bond formed by mutual association of polyphenols and the aforementioned substances, also ethanol has high solubility on polyphenol and low solubility on protein and polysaccharide, and pectin is insoluble in ethanol. Compared with the traditional extraction techniques, the extraction method provided by the invention has the advantages of simple operation, low cost, fast extraction speed, high efficiency, high purity, small energy consumption and the like, and also can reduce product pollution and thermosensitive substance decomposition.

Description

The extracting method of plant polyphenol
Technical field
The present invention relates to food processing technology field, be specifically related to the extracting method of plant polyphenol in a kind of mangrove bark.
Background technology
Mangifera Root and stem of Cholla Anacardiaceae perennial plant.Mango has another name called " hoping fruit ", namely gets " wishing fruit ".It is nutritious, and be therefore otherwise known as " king of tropical fruit ".Mangrove bark accounts for about 10% of Mango Fruit weight, is rich in the carbohydrate of needed by human body, protein, fat, also containing abundant vitamin A, B, C.Wherein the content of vitamin A is up to 3.8%.
Also containing necessary mineral elements of human body such as calcium, phosphorus, iron in mangrove bark.In addition, mangrove bark also has very high pharmaceutical use." food draft is recorded " is recorded: " the disease that mango can be controlled ' married woman's passages through which vital energy circulates is obstructed, and it is not all right that husband battalion defends invasion of vessels '." the sweet acid of mangrove bark taste, cool in nature nontoxic, there is clearing heat and promoting fluid, diuresis of quenching one's thirst, the functions such as beneficial stomach preventing or arresting vomiting.
Mangrove bark annual production is comparatively large, but the overwhelming majority but goes out of use, do not utilized.In states such as Philippines, mangrove bark and Semen Mangiferae indicae many as broiler fodder and additive thereof.
Mangrove bark also has many new functions, and certain foreign university medical center one doctor points out; Certain protein contained in mangrove bark or organic acid, can kill verrucosis poison; The experiment of the Ya Sidun University Medical institute professor Louis of Birmingham, GBR, by cyan mangrove bark powder treatment stomach ulcer, curative ratio reaches 96.4%.
The good medicine that mangrove bark is still improved looks simply.West Virginia Univ. of the U.S. one doctor finds that mangrove bark can be treated skin and to be fullyed recover from an illness overworked disease, and has very large restraining effect to Mycophyta.Effective constituent containing Antifungi or bacterium in skin can be used for treating the skin pruritus caused by fungi infestation and vitamin B1 deficiency.Mangrove bark goods can also make skin fair and clear, strengthen elasticity.But China is also less for mangrove bark research, and the domestic investigator of having extracts pectin, pigment and Fodder making from mangrove bark, but there is not been reported for the research of mangrove bark polyphenol aspect.
Because the whole content of mangrove bark polyphenol extract contained in mangrove bark is lower, materiel machining amount is larger, and due to the impact of the materials such as wherein comprised pectin, adopt extracting method of the prior art, extraction efficiency is low, cost is high, and extract moderate purity is low, foreign matter content is higher.
Summary of the invention
For the deficiency that prior art exists, the present invention seeks to the characteristic for mangrove bark, provide a kind of high-level efficiency, low cost, purity high and be easy to the extracting method of plant polyphenol in the mangrove bark promoted.
To achieve these goals, the present invention realizes by the following technical solutions:
An extracting method for plant polyphenol in mangrove bark, concrete steps are:
The first step, chopping blanching: take and shred 100 weight part mangrove barks, and by described mangrove bark blanching 0.5 in boiling water ~ minutes 2 minutes;
Second step, step one obtained mangrove bark 0.2% ~ 1.0% sodium sulfite solution and soak 0.5 ~ 2.0 minute, then add 2% ~ 1.0% vitamin c solution;
3rd step, mangrove bark water step 2 obtained are rinsed well, then add the fragmentation of ultrapure water beating equipment according to volume ratio 1:100 ~ 10:1 ratio;
Add dehydrated alcohol in 4th step, the slurry that obtains to the 3rd step, gained mixed solution is placed in 50 DEG C ~ 70 DEG C, preferably lixiviate 0.5 ~ 1.5 hour in the water bath of 60 DEG C, preferably 1.0 hours, obtains vat liquor;
The vat liquor obtained in 5th step, combining step four, then with the speed of 5000r/ minute, centrifugal 5 minutes are carried out to it, get supernatant liquor after centrifugal;
6th step, by after supernatant liquor drying, obtained mangrove bark polyphenol extract.
6) extract is reclaimed: decompression recycling ethanol, after lyophilize, obtained mangrove bark polyphenol extract.
Wherein, in described chopping blanch step, first mangrove bark is chopped into 1 × 1cm 2square thin skin.
Mangrove bark in described broken homogenizing step and the feed liquid ratio of ultrapure water are 1:5.
Slurry in described alcohol steep step and the volume ratio of dehydrated alcohol are 3:2 ~ 1:4, preferred 2:3.
Another aspect of the present invention, additionally provides a kind of mangrove bark plant polyphenol prepared according to the extracting method of above-mentioned any one.
The invention has the beneficial effects as follows:
The present invention for raw material, can overcome the impact of the materials such as pectin with waste mangrove bark preferably, adopt Non-traditional Techniques efficiently, therefrom extract the polyphenols of high-quality fast.It is water-soluble substances that the present invention takes full advantage of mangrove bark polyphenol, and its water-soluble characteristic increased with the rising of temperature, first uses water blancing.Due to pectin, polysaccharide contained in mangrove bark, the materials such as protein, the hydrogen bond adopting the ethanolic soln of setting concentration mutually to associate to polyphenol and between them to be formed and hydrophobic bond have good blocking effect, and ethanol has higher solvability to polyphenol, lower to the solvability of protein, polysaccharide, pectin is insoluble to ethanol, decreases the impurity in extract, improves extraction rate and effect simultaneously.Compared with traditional extraction technology, have easy and simple to handle, extraction rate is fast, efficiency is high, power consumption less, purity advantages of higher, simultaneously in the course of processing of the present invention, also can reduce the decomposition of pollution to product and heat-sensitive substance.
Accompanying drawing explanation
Fig. 1 is that different ethanol concentration affects schematic diagram to extraction yield;
Fig. 2 is that different extraction temperature affects schematic diagram to extraction yield;
Fig. 3 is that different extraction time affects schematic diagram to extraction yield.
Embodiment
The technique means realized for making the present invention, creation characteristic, reaching object and effect is easy to understand, below in conjunction with specific embodiment, setting forth the present invention further.
Embodiment 1
An extracting method for plant polyphenol in mangrove bark, its concrete steps are:
1) blanching is shredded: take 100 weight part mangrove barks, chopping, blanching 0.5 ~ 2 minute in boiling water;
2) deactivation polyphenoloxidase: adopt S-WAT to coordinate deactivation with vitamins C, first soak 0.5 minute with 0.2% sodium sulfite solution, and then directly add 0.2% vitamin c solution;
3) broken homogeneous: before broken, mangrove bark is first rinsed well with flowing water, adds the fragmentation of ultrapure water hollander according to setting feed liquid ratio immediately;
4) alcohol steep: according to the volume of broken disposed slurry, adds dehydrated alcohol according to setting volume ratio 1:100, gained mixed solution is placed in 40 DEG C of water bath lixiviate 0.5h in slurry;
5) centrifugal: merging filtrate, then in the speed of 5000r/ minute it is carried out process in centrifugal 5 minutes centrifugal after get supernatant liquor;
6) extract is reclaimed: decompression recycling ethanol, after lyophilize, obtained mangrove bark polyphenol extract.
At described chopping blanch step 1) in, first mangrove bark is chopped into 1 × 1cm 2square thin skin.
At described broken homogenizing step 3) in, the feed liquid ratio of described mangrove bark and ultrapure water is 1:5.
In described alcohol steep step 4) in, described slurry and the volume ratio of dehydrated alcohol are 3:2.
Embodiment 2
An extracting method for plant polyphenol in mangrove bark, concrete steps are:
1) blanching is shredded; Take 100g mangrove bark, chopping, blanching 0.5 minute in boiling water;
2) deactivation polyphenoloxidase: S-WAT coordinates with vitamin c, first soaks 1 minute with 0.5% sodium sulfite solution, then directly adds 0.5% vitamin c solution;
3) broken homogeneous: before broken, mangrove bark is first rinsed well with flowing water, adds the fragmentation of ultrapure water hollander according to 1:5 feed liquid ratio immediately;
4) alcohol steep: according to the volume of broken disposed slurry, adds dehydrated alcohol according to setting volume ratio, this mixed solution is placed in 70 DEG C of water bath lixiviate 1.0h in slurry;
5) centrifugal: merging filtrate, then in the speed of 5000r/ minute it is carried out process in centrifugal 5 minutes centrifugal after get supernatant liquor;
6) extract is reclaimed: decompression recycling ethanol, after lyophilize, obtained mangrove bark polyphenol extract.
The affect experimental result of different ethanol concentration on extraction yield is shown in Fig. 1.As shown in Figure 1, when ethanol content is 60%, when namely the volume ratio of slurry and dehydrated alcohol is 2:3, extraction effect is best.This is relevant with the component of mango and the physical properties of polyphenol.Polyphenol is soluble in ethanol, the material compound such as protein, alkaloid, polysaccharide, anthocyanogen of plant polyphenol easily and in plant tissue.The mixed solution of organic solvent and water can interrupt the associative key of polyphenols and the material such as protein, polysaccharide, is conducive to the extraction of polyphenol.But alcohol concn is too high can be made again Semen Mangiferae indicae cause because of dehydration between fiber to tighten, affect oozing out of polyphenol.
Embodiment 3
An extracting method for plant polyphenol in mangrove bark, concrete steps are:
1) blanching is shredded: take 100g mangrove bark, chopping, blanching 2.0 minutes in boiling water;
2) deactivation polyphenoloxidase: S-WAT coordinates with vitamin c, 2.0% S-WAT soaks 2 minutes, then directly adds 1.0% vitamins C;
3) broken homogeneous: before broken, mangrove bark is first rinsed well with flowing water, adds the fragmentation of ultrapure water hollander according to 1:5 feed liquid ratio immediately;
4) alcohol steep: according to the volume of broken disposed slurry, adds dehydrated alcohol according to 1:4 volume ratio in slurry, this mixed solution is placed in certain temperature water bath lixiviate 1.5h;
5) centrifugal: merging filtrate, then in the speed of 5000r/ minute it is carried out process in centrifugal 5 minutes centrifugal after get supernatant liquor;
6) extract is reclaimed: decompression recycling ethanol, lyophilize obtains mangrove bark polyphenol extract.
The affect experimental result of different extraction temperatures on extraction yield is shown in Fig. 2.As shown in Figure 2, from the impact on extraction yield, the rising productive rate along with bath temperature first rises and declines afterwards, reaches best 60 DEG C time.This is because along with the rising of temperature, molecular motion velocities is accelerated, and is conducive to oozing out of polyphenol, but temperature is too high can destroy the molecular structure of polyphenol thus have impact on extraction yield.
Embodiment 4
An extracting method for plant polyphenol in mangrove bark, concrete steps are:
1) blanching is shredded: take 100g mangrove bark, chopping, blanching 0.5 minute in boiling water;
2) deactivation polyphenoloxidase: S-WAT coordinates with vitamin c, adopts 0.5% sodium sulfite solution to soak 1 minute, then directly adds 0.5% vitamin c solution;
3) broken homogeneous: before broken, mangrove bark is first rinsed well with flowing water, adds the fragmentation of ultrapure water hollander according to 1:5 feed liquid ratio immediately;
4) alcohol steep: according to the volume of broken disposed slurry, adds dehydrated alcohol according to 2:3 volume ratio in slurry, this mixed solution is placed in 60 DEG C of water bath lixiviate certain hours;
5) centrifugal: merging filtrate, then in the speed of 5000r/ minute it is carried out process in centrifugal 5 minutes centrifugal after get supernatant liquor;
6) extract is reclaimed: decompression recycling ethanol, lyophilize obtains mangrove bark polyphenol extract.
The affect experimental result of different extraction times on extraction yield is shown in Fig. 3.As shown in Figure 3, extract yield when 1.0h and reach the highest.This is because polyphenol can be made fully to be dissolved in solvent along with the lengthening of return time.But overlong time can destroy the molecular structure of polyphenol because of the impact of temperature.
Embodiment 5
An extracting method for plant polyphenol in mangrove bark, concrete steps are:
1) blanching is shredded; Take 100g part mangrove bark, chopping, blanching 0.5 minute in boiling water;
2) deactivation polyphenoloxidase: S-WAT coordinates with vitamin c, 0.5% S-WAT soaks 1 minute, then directly adds 0.5% vitamins C;
3) broken homogeneous: before broken, mangrove bark is first rinsed well with flowing water, adds the fragmentation of ultrapure water hollander according to 1:5 feed liquid ratio immediately;
4) alcohol steep: according to the volume of broken disposed slurry, adds dehydrated alcohol according to certain volume ratio in slurry, this mixed solution is placed in 60 DEG C of water bath lixiviate 1.0h;
5) centrifugal: merging filtrate, then in the speed of 5000r/ minute it is carried out process in centrifugal 5 minutes centrifugal after get supernatant liquor;
6) extract is reclaimed: decompression recycling ethanol, lyophilize obtains mangrove bark polyphenol extract 7.4g.
Wonderful discovery adopts the yield of polyphenol extract in the embodiment of method of the present invention significantly higher than the yield P<0.05 of polyphenol extract in art methods.
What adopt in each embodiment above is all weight ratio, or mass percent concentration.
More than show and describe ultimate principle of the present invention and principal character and advantage of the present invention.The technician of the industry should understand; the present invention is not restricted to the described embodiments; what describe in above-described embodiment and specification sheets just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.Application claims protection domain is defined by appending claims and equivalent thereof.

Claims (5)

1. the extracting method of plant polyphenol in mangrove bark, it comprises the steps:
The first step, take and shred 100 weight part mangrove barks, and by the blanching 0.5 minute ~ 2.0 minutes in boiling water of described mangrove bark;
Second step, step one obtained mangrove bark 0.2% ~ 1.0% sodium sulfite solution and soak 0.5 ~ 2.0 minute, then add 0.2% ~ 1.0% vitamin c solution;
3rd step, mangrove bark water step 2 obtained are rinsed well, then add the fragmentation of ultrapure water beating equipment according to volume ratio 1:100 ~ 10:1 ratio;
Add dehydrated alcohol in 4th step, the slurry that obtains to the 3rd step, gained mixed solution is placed in 50 DEG C ~ 70 DEG C, preferably lixiviate 0.5 ~ 1.5 hour in the water bath of 60 DEG C, preferably 1.0 hours, obtains vat liquor;
The vat liquor obtained in 5th step, combining step four, then with the speed of 5000r/ minute, centrifugal 5 minutes are carried out to it, get supernatant liquor after centrifugal;
6th step, by after supernatant liquor drying, obtained mangrove bark polyphenol extract.
2. the extracting method of plant polyphenol in mangrove bark according to claim 1, is characterized in that: before chopping blanch step one, first mangrove bark is chopped into 1 × 1cm 2square thin skin.
3. the extracting method of plant polyphenol in mangrove bark according to claim 1, it is characterized in that: in described step 3, the ratio of described mangrove bark and ultrapure water is volume ratio 1:5.
4. the extracting method of plant polyphenol in mangrove bark according to claim 1, it is characterized in that: in described step 4, described slurry and the volume ratio of dehydrated alcohol are 3:2 ~ 1:4, preferred 2:3.
5. mangrove bark plant polyphenol prepared by the extracting method any one of Claims 1-4.
CN201510747582.4A 2015-11-05 2015-11-05 Extraction method of plant polyphenol Pending CN105348331A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105878306A (en) * 2016-04-11 2016-08-24 浙江中医药大学 Preparation method of pomegranate peel polyphenols
CN106344634A (en) * 2016-11-14 2017-01-25 海南医学院 Mango peel extract and preparation method and application thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102516319A (en) * 2011-12-08 2012-06-27 广西大学 Method for extracting phenolic compounds from sugarcane tips
CN104840587A (en) * 2015-04-02 2015-08-19 广西大学 Method for extracting longan seed polyphenol
CN104974194A (en) * 2015-06-29 2015-10-14 广西大学 Technology for extracting polyphenol from grape peel residues

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102516319A (en) * 2011-12-08 2012-06-27 广西大学 Method for extracting phenolic compounds from sugarcane tips
CN104840587A (en) * 2015-04-02 2015-08-19 广西大学 Method for extracting longan seed polyphenol
CN104974194A (en) * 2015-06-29 2015-10-14 广西大学 Technology for extracting polyphenol from grape peel residues

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
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李建强,等: "芒果皮中功效成分应用研究进展", 《食品研究与开发》 *
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105878306A (en) * 2016-04-11 2016-08-24 浙江中医药大学 Preparation method of pomegranate peel polyphenols
CN106344634A (en) * 2016-11-14 2017-01-25 海南医学院 Mango peel extract and preparation method and application thereof
CN106344634B (en) * 2016-11-14 2019-09-24 海南医学院 A kind of mango bark extract and its preparation method and application

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