CN103550275A - Method for extracting saussurea involucrate essences - Google Patents
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Abstract
The invention discloses a method for extracting saussurea involucrate essences. The method comprises the following steps: (1) preprocessing saussurea involucrate and purifying water; (2) extracting the preprocessed saussurea involucrate and the purified water; (3) coarsely filtering cooled saussurea involucrate soup and precisely separating; (4) filtering precisely filtered liquor by a microporous membrane with the aperture being 0.25-0.45 microns; (5) concentrating refined purifying liquid by a reverse osmosis membrane at normal temperature; (6) carrying out reduced pressure concentration on the ginseng fruit soup concentrated by the reverse osmosis membrane; and (7) finally obtaining the saussurea involucrate essences. Compared with the prior art, the method can be used for extracting the saussurea involucrate essences, greatly increases the nutrition absorption rate, and has the advantages of high efficiency, environmental friendliness, safety, capability of ensuring activity of effective ingredients and the like; the saussurea involucrate essences keep the flavor and the nutritional ingredients of the saussurea involucrate, are convenient to carry, and can be used for bringing the effects of the precious saussurea involucrate into full play.
Description
Technical field
The present invention relates to field of health care products, exactly refer to a kind of extracting method of Herba Saussureae Involueratae elite material.
Background technology
Herba Saussureae Involueratae is the herbaceous plant of Compositae phoenix hair Chrysanthemum Herba Saussureae Involueratae subgenus.It is grown between the rock flowing hillside of high mountains and near rubble snow line of 4800-5800 meters of height above sea level.The plant of this subgenus has more than 20 to plant, and the overwhelming majority originates in Chinese Tibetan Plateau and its adjacent regions.Therefore Herba Saussureae Involueratae is the rare famous and precious medicinal plants of a kind of high mountain, protects Herba Saussureae Involueratae germ plasm resource, in science, pharmaceutically or all significant from viewing and admiring.
Herba Saussureae Involueratae is grown in below high mountain snow line conventionally.Climate variability, cold and hot impermanence, sleet replaces, the highest monthly average temperature 3--5 ℃, minimum monthly average temperature-19~21 ℃, approximately 800 millimeters of annual precipitations, frost-free period only had about 50 days.Soil be take alpine meadow soil as main, and the content of organic matter is 8.5--11%, nitrogen content 4.5--10%.Because environmental condition is severe, general plant is difficult to growth, only has cold-resistant, the low temperature resistant Carex Carexspp of minority, and high grass belongs to Kobresia spp. and the association with it of various high mountain herbaceos perennials.Herba Saussureae Involueratae is under this high mountain rigor condition, and poor growth, at least just can yield positive results after 4--5.But because trophophase is short, it can germinate rapidly in the short period of time, growth, Flowering and fruiting.July at florescence, really August phase.4000 meters of 2400 meters of height above sea level upper limits of growing environment height above sea level lower limit.
Herba Saussureae Involueratae has the biological characteristics that adapts to Alpine environment, the extremely close shape of its leaf is as white long draft hair, as if silk tops, MIAOMAO interweaves, the gas having formed in countless " cell " chambers is difficult to exchange with the external world, daytime, the heat that it absorbs than soil around and air was large under the direct irradiation of sunlight; And MIAOMAO layer can make body exempt from the injury of intense radiation.In addition, intensive in the head inflorescence on stem top, often mend the close blade by long draft hair in two sides and seal, just as having put on white sweater, under cold Alpine environment, propagate and connect generation guaranteeing.The characteristic of this adaptation Alpine environment of Herba Saussureae Involueratae is that it forms for a long time under the cold and arid condition of high mountain.Due to the thin intracellular accumulation of Herba Saussureae Involueratae the materials such as a large amount of soluble sugars, protein and lipid, can make the apparent freezing point of cellular plasm liquid reduce, when temperature drops to paraplasm when below freezing, intraprotoplasmic moisture is just penetrated in intercellular substance and plasmolytic space and freezes.And protoplast dwindles gradually, can not suffer damage.When the same day, cyclostrophic was warm, ice cube melts, and moisture is absorbed by protoplast again, and cell returns to again the acrophyta that normality is peculiar---and Herba Saussureae Involueratae is exactly by this cold-resistant characteristic, survives in high and cold mountain.
Herba Saussureae Involueratae also has distribution except producing Xinjiang, Qinghai, Sichuan, the Yunnan of Tibet Wai, China.Various places are among the people by Herba Saussureae Involueratae all herbal medicine, cure mainly snow the region between the heart and the diaphragm, toothache, rheumatic arthritis, sexual impotence, menoxenia, redly collapse, the disease such as leucorrhea.India is among the people also treats many chronics with Herba Saussureae Involueratae.As diseases such as gastric ulcer, hemorrhoid, bronchitis, heart disease, epistaxis and venom.On Tibetan medicine, Herba Saussureae Involueratae is as the existing long history of medicine.All on the books on tetra-doctor's allusion quotation > > of the precious > > of Tibetanmedicine document < < month king medicine and < <.
Herba Saussureae Involueratae has physiologically active effective ingredient.Wherein umbelliferone have significantly antibiotic, blood pressure lowering is calm, spasmolysis; Scopoletin has and dispels the wind, antiinflammatory, pain relieving, eliminates the phlegm and antitumor action, and treating clinically dyspnea with rapid respiration chronic bronchitis effective percentage is that 96.6% apigenin has smooth muscle spasmolysis and anti-ulcer effect; Antelope base benzophenone is had to obvious choleretic effect.Full of interest is especially colchicine contained in Herba Saussureae Involueratae, this composition is a Typical Representative of cell mitogen, and the growth of energy anticancer is clinical in to treat cancer, with breast carcinoma, there is certain curative effect especially, skin carcinoma, leukemia and what king's evil etc. are also had to certain effect.To the special effect of gout acute attack, in 12-24 hours, reduce inflammation and pain relieving rapidly, life-time service can reduce attack times.
There is in addition estrogen-like effects activity, energy prolong rats rutting period and metoestrus, and shorten dioestrus and proestrus.But the toxicity of colchicine is larger, can cause nausea, loss of appetite, abdominal distention, severe patient there will be the side effect such as enteroparalysis and constipation, extremities aching pain.The larger colchicine of toxicity owing to containing good effect in Herba Saussureae Involueratae, is steeping in wine with Herba Saussureae Involueratae while curing mainly rheumatic arthritis and gynaecopathia and can't take more so among the people.
At present, the eating method of most Herba Saussureae Involueratae, for cooking, is steep in wine or fricassee on the market, but above directly eating method can only absorb hundred parts of Herba Saussureae Involueratae nutrition 20 to 40, central hundred parts 60 to 80 will therefore waste.
Summary of the invention
For above-mentioned defect, the technical problem that the present invention solves is to provide a kind of extracting method of Herba Saussureae Involueratae elite material, extract the elite of Herba Saussureae Involueratae, greatly promote alimentation rate, there is high efficiency, environmental protection, safety and guarantee the advantages such as effective ingredient activity, both the taste and the nutrient that had kept Herba Saussureae Involueratae, be easy to carry again, famous and precious Herba Saussureae Involueratae efficacy effect can be brought into play completely.
In order to solve above technical problem, the extracting method of Herba Saussureae Involueratae elite material provided by the invention, comprises the steps:
(1) pretreatment of Herba Saussureae Involueratae, removes the impurity on Herba Saussureae Involueratae surface with staff, lower than the temperature of 60 ℃, with pulverizer, Herba Saussureae Involueratae is being pulverized, and fineness requirement is at 8 orders or following, and particle diameter is the smaller the better; To extracting, with former water, process, use the pure water of removing ion;
(2) Herba Saussureae Involueratae pretreatment being obtained and pure water are in the Herba Saussureae Involueratae of 1:8-1:15 weight portion and water ratio, water temperature extracts at 20 ℃-50 ℃, and extraction is carried out 2-4 time, each 10-60 minute, the Herba Saussureae Involueratae soup filtering out after extraction is several times merged, cooling at ambient temperature;
(3) will after the coarse filtration of cooled Herba Saussureae Involueratae soup, carry out precision separation, by aperture, at the accurate membrane filtration of 3-10um, obtain the secondary filter liquid of Herba Saussureae Involueratae soup;
(4) by secondary filter liquid, with aperture, be 0.25-0.45um micro-pore-film filtration again, obtain the refining refined solution of Herba Saussureae Involueratae soup;
(5) refining refined solution is concentrated at normal temperatures to the concentrated Herba Saussureae Involueratae soup that to obtain solid content be 8-15% with reverse osmosis membrane;
(6) by the concentrated Herba Saussureae Involueratae soup of process reverse osmosis membrane, then carry out concentrating under reduced pressure, adopt the low temperature of 40 ℃-50 ℃, it is 50-70% that Herba Saussureae Involueratae soup is concentrated to solid content;
(7) concentrated solution is carried out to microwave vacuum cold drying, vacuum lyophilization or spray-dired mode, finally obtain elite extract.
Preferably, in step (1), at the temperature of 20-50 ℃, with pulverizer, Herba Saussureae Involueratae is pulverized.
Preferably, described elite extract is made electuary, tablet or capsule.
Preferably, residue microwave vacuum cold drying, vacuum lyophilization or spraying being obtained after dry is made highland barley biscuit.
Compared with prior art, the extracting method of Herba Saussureae Involueratae elite material provided by the invention, the elite of using low-temperature extraction isolation technics to extract Herba Saussureae Involueratae can bring up to hundred parts 70%, low-temperature extraction isolation technics is by the low-temperature extraction technology in conjunction with shearing and vibration force effect, combined type microwave-assisted extraction technique and supercritical carbon dioxide extraction isolation technics three technology are integrated and are formed, this technology can combine adjustment according to the characteristic of raw material and product demand, there is high efficiency, environmental protection, safety with guarantee the advantages such as effective ingredient activity, the scope of application is quite extensive, operative temperature is lower than 60 ℃, can guarantee the activity of effective ingredient in natural goods, do not use the solvent of harmful human body, in economic mode, carry out extract and separate, can fully retain its effective ingredient and fragrance, equipment is easy to amplify, emphasizing the modern life healthy and health preserving, coordinate low-temperature extraction isolation technics that the efficacy exertion of Herba Saussureae Involueratae is arrived maximum, greatly promote alimentation rate, there is high efficiency, environmental protection, safety with guarantee the advantages such as effective ingredient activity, both the taste and the nutrient that had kept Herba Saussureae Involueratae, be easy to carry again, famous and precious Herba Saussureae Involueratae efficacy effect can be brought into play completely.
Accompanying drawing explanation
Fig. 1 is the flow chart of the extracting method of Herba Saussureae Involueratae elite material in the present invention.
The specific embodiment
For those skilled in the art can understand technical scheme provided by the present invention better, below in conjunction with specific embodiment, set forth.
Refer to Fig. 1, this figure is the structural representation of the extracting method of Herba Saussureae Involueratae elite material in the present invention.
Embodiment 1
The extracting method of the Herba Saussureae Involueratae elite material that the embodiment of the present invention provides, comprises the steps:
(1) pretreatment of Herba Saussureae Involueratae, removes the impurity on Herba Saussureae Involueratae surface with staff, the temperature of 50 ℃, with pulverizer, Herba Saussureae Involueratae is pulverized, and fineness requirement is at 8 orders; To extracting, with former water, process, use the pure water of removing ion;
(2) Herba Saussureae Involueratae pretreatment being obtained and pure water are in the Herba Saussureae Involueratae of 1:8 weight portion and water ratio, and water temperature extracts at 20 ℃, and extraction is carried out 3 times, and each 30 minutes, the Herba Saussureae Involueratae soup filtering out after 4 extractions is merged, cooling at ambient temperature;
(3) will after the coarse filtration of cooled Herba Saussureae Involueratae soup, carry out precision separation, by aperture, at the accurate membrane filtration of 8um, obtain the secondary filter liquid of Herba Saussureae Involueratae soup;
(4) by secondary filter liquid, with aperture, be 0.25um micro-pore-film filtration again, obtain the refining refined solution of Herba Saussureae Involueratae soup;
(5) refining refined solution is concentrated at normal temperatures to the concentrated Herba Saussureae Involueratae soup that to obtain solid content be 10% with reverse osmosis membrane;
(6) by the concentrated Herba Saussureae Involueratae soup of process reverse osmosis membrane, then carry out concentrating under reduced pressure, adopt the low temperature of 50 ℃, it is 70% that Herba Saussureae Involueratae soup is concentrated to solid content;
(7) concentrated solution is carried out to microwave vacuum cold drying, vacuum lyophilization or spray-dired mode, finally obtain elite extract, elite extract is made electuary; The residue obtaining after microwave vacuum cold drying, vacuum lyophilization or spraying is dry is made highland barley biscuit.
The 10 grams of Herba Saussureae Involueratae of take are example, and the elite of extracting wherein with low-temperature extraction isolation technics has 72%, only remaining 28% residue.
Embodiment 2
The extracting method of the Herba Saussureae Involueratae elite material that the embodiment of the present invention provides, comprises the steps:
(1) pretreatment of Herba Saussureae Involueratae, removes the impurity on Herba Saussureae Involueratae surface with staff, the temperature of 30 ℃, with pulverizer, Herba Saussureae Involueratae is pulverized, and fineness requirement is at 20 orders; To extracting, with former water, process, use the pure water of removing ion;
(2) Herba Saussureae Involueratae pretreatment being obtained and pure water are in the Herba Saussureae Involueratae of 1:15 weight portion and water ratio, and water temperature extracts at 50 ℃, and extraction is carried out 4 times, and each 60 minutes, the Herba Saussureae Involueratae soup filtering out after 4 extractions is merged, cooling at ambient temperature;
(3) will after the coarse filtration of cooled Herba Saussureae Involueratae soup, carry out precision separation, by aperture, at the accurate membrane filtration of 10um, obtain the secondary filter liquid of Herba Saussureae Involueratae soup;
(4) by secondary filter liquid, with aperture, be 0.45um micro-pore-film filtration again, obtain the refining refined solution of Herba Saussureae Involueratae soup;
(5) refining refined solution is concentrated at normal temperatures to the concentrated Herba Saussureae Involueratae soup that to obtain solid content be 15% with reverse osmosis membrane;
(6) by the concentrated Herba Saussureae Involueratae soup of process reverse osmosis membrane, then carry out concentrating under reduced pressure, adopt the low temperature of 50 ℃, it is 50% that Herba Saussureae Involueratae soup is concentrated to solid content;
(7) concentrated solution is carried out to microwave vacuum cold drying, vacuum lyophilization or spray-dired mode, finally obtain elite extract, elite extract is made capsule; The residue obtaining after microwave vacuum cold drying, vacuum lyophilization or spraying is dry is made highland barley biscuit.
The 10 grams of Herba Saussureae Involueratae of take are example, and the elite of extracting wherein with low-temperature extraction isolation technics has 70%, only remaining 30% residue.
Embodiment 3
The extracting method of the Herba Saussureae Involueratae elite material that the embodiment of the present invention provides, comprises the steps:
(1) pretreatment of Herba Saussureae Involueratae, removes the impurity on Herba Saussureae Involueratae surface with staff, the temperature of 20 ℃, with pulverizer, Herba Saussureae Involueratae is pulverized, and fineness requirement is at 100 orders; To extracting, with former water, process, use the pure water of removing ion;
(2) Herba Saussureae Involueratae pretreatment being obtained and pure water are in the Herba Saussureae Involueratae of 1:12 weight portion and water ratio, and water temperature extracts at 40 ℃, and extraction is carried out 2 times, and each 30 minutes, the Herba Saussureae Involueratae soup filtering out after 2 extractions is merged, cooling at ambient temperature;
(3) will after the coarse filtration of cooled Herba Saussureae Involueratae soup, carry out precision separation, by aperture, at the accurate membrane filtration of 5um, obtain the secondary filter liquid of Herba Saussureae Involueratae soup;
(4) by secondary filter liquid, with aperture, be 0.35um micro-pore-film filtration again, obtain the refining refined solution of Herba Saussureae Involueratae soup;
(5) refining refined solution is concentrated at normal temperatures to the concentrated Herba Saussureae Involueratae soup that to obtain solid content be 10% with reverse osmosis membrane;
(6) by the concentrated Herba Saussureae Involueratae soup of process reverse osmosis membrane, then carry out concentrating under reduced pressure, adopt the low temperature of 45 ℃, it is 60% that Herba Saussureae Involueratae soup is concentrated to solid content;
(7) concentrated solution is carried out to microwave vacuum cold drying, vacuum lyophilization or spray-dired mode, finally obtain elite extract, elite extract is made tablet; The residue obtaining after microwave vacuum cold drying, vacuum lyophilization or spraying is dry is made highland barley biscuit.
The 10 grams of Herba Saussureae Involueratae of take are example, and the elite of extracting wherein with low-temperature extraction isolation technics has 75%, only remaining 25% residue.
Compared with prior art, the extracting method of Herba Saussureae Involueratae elite material provided by the invention, the elite of using low-temperature extraction isolation technics to extract Herba Saussureae Involueratae can bring up to hundred parts 70%, low-temperature extraction isolation technics is by the low-temperature extraction technology in conjunction with shearing and vibration force effect, combined type microwave-assisted extraction technique and supercritical carbon dioxide extraction isolation technics three technology are integrated and are formed, this technology can combine adjustment according to the characteristic of raw material and product demand, there is high efficiency, environmental protection, safety with guarantee the advantages such as effective ingredient activity, the scope of application is quite extensive, operative temperature is lower than 60 ℃, can guarantee the activity of effective ingredient in natural goods, do not use the solvent of harmful human body, in economic mode, carry out extract and separate, can fully retain its effective ingredient and fragrance, equipment is easy to amplify, emphasizing the modern life healthy and health preserving, coordinate low-temperature extraction isolation technics that the efficacy exertion of Herba Saussureae Involueratae is arrived maximum, greatly promote alimentation rate, there is high efficiency, environmental protection, safety with guarantee the advantages such as effective ingredient activity, both the taste and the nutrient that had kept Herba Saussureae Involueratae, be easy to carry again, famous and precious Herba Saussureae Involueratae efficacy effect can be brought into play completely.
Above-mentioned explanation to the disclosed embodiments, makes professional and technical personnel in the field can realize or use the present invention.To the multiple modification of these embodiment, will be apparent for those skilled in the art, General Principle as defined herein can, in the situation that not departing from the spirit or scope of the present invention, realize in other embodiments.Therefore, the present invention will can not be restricted to these embodiment shown in this article, but will meet the widest scope consistent with principle disclosed herein and features of novelty.
Claims (4)
1. an extracting method for Herba Saussureae Involueratae elite material, is characterized in that, comprises the steps:
(1) pretreatment of Herba Saussureae Involueratae, removes the impurity on Herba Saussureae Involueratae surface with staff, lower than the temperature of 60 ℃, with pulverizer, Herba Saussureae Involueratae is being pulverized, and fineness requirement is at 8 orders or following; To extracting, with former water, process, use the pure water of removing ion;
(2) Herba Saussureae Involueratae pretreatment being obtained and pure water are in the Herba Saussureae Involueratae of 1:8-1:15 weight portion and water ratio, water temperature extracts at 20 ℃-50 ℃, and extraction is carried out 2-4 time, each 10-60 minute, the Herba Saussureae Involueratae soup filtering out after extraction is several times merged, cooling at ambient temperature;
(3) will after the coarse filtration of cooled Herba Saussureae Involueratae soup, carry out precision separation, by aperture, at the accurate membrane filtration of 3-10um, obtain the secondary filter liquid of Herba Saussureae Involueratae soup;
(4) by secondary filter liquid, with aperture, be 0.25-0.45um micro-pore-film filtration again, obtain the refining refined solution of Herba Saussureae Involueratae soup;
(5) refining refined solution is concentrated at normal temperatures to the concentrated Herba Saussureae Involueratae soup that to obtain solid content be 8-15% with reverse osmosis membrane;
(6) by the concentrated Herba Saussureae Involueratae soup of process reverse osmosis membrane, then carry out concentrating under reduced pressure, adopt the low temperature of 40 ℃-50 ℃, it is 50-70% that Herba Saussureae Involueratae soup is concentrated to solid content;
(7) concentrated solution is carried out to microwave vacuum cold drying, vacuum lyophilization or spray-dired mode, finally obtain elite extract.
2. the extracting method of Herba Saussureae Involueratae elite material according to claim 1, is characterized in that, in step (1), at the temperature of 20-50 ℃, with pulverizer, Herba Saussureae Involueratae is pulverized.
3. the extracting method of Herba Saussureae Involueratae elite material according to claim 1, is characterized in that, described elite extract is made electuary, tablet or capsule.
4. the extracting method of Herba Saussureae Involueratae elite material according to claim 1, is characterized in that, the residue obtaining after microwave vacuum cold drying, vacuum lyophilization or spraying are dried is made highland barley biscuit.
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Cited By (3)
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CN104397683A (en) * | 2014-10-31 | 2015-03-11 | 广州丹奇日用化工厂有限公司 | Method for extracting Saussurea medusa essence materials |
CN108553498A (en) * | 2018-04-24 | 2018-09-21 | 烟台金蕊女性用品有限公司 | A kind of saussurea involucrata essence extraction process |
CN115028751A (en) * | 2022-06-15 | 2022-09-09 | 中国科学院新疆理化技术研究所 | Preparation method and application of saussurea involucrate polysaccharide |
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CN102106889A (en) * | 2011-02-21 | 2011-06-29 | 贾正平 | Application of snow saussurea and snow saussurea extract in preparation of anti-hypoxia medicaments and method for preparing snow saussurea extract |
CN103191166A (en) * | 2013-03-25 | 2013-07-10 | 西藏藏真堂藏药产业有限公司 | Method for increasing yield of saussurea obvallata, extract of saussurea obvallata and application of extract |
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CN1759710A (en) * | 2005-11-09 | 2006-04-19 | 云南省农业机械研究所 | 'Puer' caffeine and processing method |
CN102106889A (en) * | 2011-02-21 | 2011-06-29 | 贾正平 | Application of snow saussurea and snow saussurea extract in preparation of anti-hypoxia medicaments and method for preparing snow saussurea extract |
CN103191166A (en) * | 2013-03-25 | 2013-07-10 | 西藏藏真堂藏药产业有限公司 | Method for increasing yield of saussurea obvallata, extract of saussurea obvallata and application of extract |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
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CN104397683A (en) * | 2014-10-31 | 2015-03-11 | 广州丹奇日用化工厂有限公司 | Method for extracting Saussurea medusa essence materials |
CN104397683B (en) * | 2014-10-31 | 2017-01-11 | 广州巴宝莉化妆品有限公司 | Method for extracting Saussurea medusa essence materials |
CN108553498A (en) * | 2018-04-24 | 2018-09-21 | 烟台金蕊女性用品有限公司 | A kind of saussurea involucrata essence extraction process |
CN115028751A (en) * | 2022-06-15 | 2022-09-09 | 中国科学院新疆理化技术研究所 | Preparation method and application of saussurea involucrate polysaccharide |
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