CN104663858A - Method for extracting biological fresh-keeping agent from honeysuckle, and applications of biological fresh-keeping agent in grape fresh keeping - Google Patents
Method for extracting biological fresh-keeping agent from honeysuckle, and applications of biological fresh-keeping agent in grape fresh keeping Download PDFInfo
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- CN104663858A CN104663858A CN201310610624.0A CN201310610624A CN104663858A CN 104663858 A CN104663858 A CN 104663858A CN 201310610624 A CN201310610624 A CN 201310610624A CN 104663858 A CN104663858 A CN 104663858A
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Abstract
The present invention relates to a method for extracting a biological fresh-keeping agent from honeysuckle. The method comprises: 1, sorting honeysuckle, and removing leaves, branches and other debris; 2, crushing the honeysuckle by using a crusher; 3, adding the crushed honeysuckle to an ethanol solution according to a material liquid ratio of 1:15-20, uniformly shaking, and transferring to a microwave extraction instrument to carry out microwave extraction, wherein the extraction temperature is 65-75 DEG C, the working time is 5-10 min, and the microwave power is 500-700 w; and 4, filtering the extraction solution to obtain the filtrate, and storing at a temperature of 2-4 DEG C. The present invention further provides applications of the biological fresh-keeping agent extracted according to the method of the present invention in grape fresh keeping. According to the present invention, the honeysuckle is adopted as the raw material to extract the biological fresh-keeping agent to carry out fresh keeping on the grapes, such that advantages of safety, no toxicity and no pollution are provided, the biological fresh-keeping agent can be used for replacing the chemical bactericidal agent and the preservative so as to be used for fresh keeping on the grapes, and the problems of environmental pollution, food safety and the like of the chemical bactericidal agent are avoided.
Description
Technical field
The present invention relates to the field of preserving fruit and vegetable utilizing, the bio-preservative extracted from honeysuckle specifically and the application in grape fresh-keeping thereof.
Background technology
Honeysuckle, has another name called two flowers, and be dry flower or the flower just opened of Caprifoliaceae woodbine honeysuckle, in China, most area all has production, is China's traditional Chinese medicine material, taste is sweet, cold in nature, clearing heat and detoxicating, wind-heat dissipating.Modern chemical analysis and pharmacological evaluation prove, honeysuckle have broad-spectrum antiseptic, antiviral, antitumor, strengthen immunity and antipyretic and anti-inflammatory, cholagogic, protect the liver, lipopenicillinase, antifertility, hemostasis, the pharmacological action such as antiulcer.It is reported, the whole nation has the bud of more than 20 Plants to be used as medicine when honeysuckle, is all Caprifoliaceae woodbine.Owing to having special drug effect, therefore become the study hotspot of Chinese herbal medicine in recent years.Containing cyanidenon in honeysuckle, isochlorogenic acid, the multiple compounds such as chlorogenic acid and flavones, tradition thinks that chlorogenic acid is the principle active component of honeysuckle, but show according to modern medicine study, flavone compound wherein has reduction MCO, make coronary artery, cerebrovascular flow increases, the anti-rhythm of the heart, softening blood vessel, hypoglycemic, the effects such as blood fat, simultaneously it or a kind of natural antioxidant, there is Superoxide anion free radical in removing human body anti-ageing, increase the physiological activity of immunity of organisms, therefore its biologically active becomes the focus of people's research day by day.
Containing mineral calcium, potassium, phosphorus, iron, protein and multivitamin in grape, the amino acid also containing multiple needed by human body, normal food grape is of great advantage to neurasthenia, excessive fatigue.After grape is made raisins, sugar can be relative high with the content of iron, is the excellent tonic product of women, children and weak anaemia person.Relax the muscles and stimulate the blood circulation from the angle grape of the traditional Chinese medical science, Appetizing spleen-tonifying, the effect such as aid digestion, its iron-holder is enriched, so enrich blood.Grape also can be used for dietotherapy: when dizziness, palpitaition, cerebral anemia, drinks appropriate grape wine every day 2-3 time, has certain therapeutic action; Dry grapevine 15 grams can control pernicious vomiting with being decocted in water for oral dose.In addition, modern medicine study shows, grape also has anti-cancer, anticancer effect.Grape also can be used to wine brewing.The huge economic value of grape is mainly wine brewing, and the grape in the whole world 80% is all for wine brewing.
Grape belongs to berries fruit, delicious flavour, rich in nutritive value, but the respiratory intensity of grape is large, and protective tissue is poor, and preservation and freshness is more difficult, and preservation term and shelf life are all shorter, can bring great loss like this for orchard worker and consumer.And the preservation method of berries fruit conventional at present mainly adopts chemical bactericide, and a large amount of uses of chemical bactericide, pathogenic microorganism can be caused to produce drug resistance, and environmental pollution; In addition, because grape cleaning is more difficult, be difficult to thoroughly wash away chemical bactericide, after edible, health hidden danger can be caused.
Summary of the invention
According to above-mentioned weak point, the object of this invention is to provide and a kind ofly extract the method for bio-preservative and the application in grape fresh-keeping thereof with the flower of honeysuckle and/leaf.
For achieving the above object, technical scheme of the present invention is: a kind of method extracting bio-preservative from honeysuckle, comprises the steps, 1, honeysuckle is cleaned, and removing leaf, branch and other foreign material; 2, pulverizer is adopted to pulverize honeysuckle; 3, be that the ratio of 1:15-20 adds ethanolic solution with solid-liquid ratio by the honeysuckle of pulverizing, shake up, and be transferred to together in Microwave Extraction Apparatus and carry out Microwave Extraction, Extracting temperature is 65-75 DEG C, and the working time is 5-10min, and microwave power is 500-700w; 4, by extracting liquid filtering, filtrate is obtained, and in 2-4 DEG C of storage.
Preferably: described ethanol solution concentration is 50-60%.
Preferably: add rotor in described leaching process and stir.
The present invention also provides a kind of application of bio-preservative in grape fresh-keeping of extracting according to method described in claim 1, immerses 5min in bio-preservative, dry, then preserve with after PE-PP composite waterproofing membrane after the grape newly plucked being rejected insect pest and mechanical damage fruit; Duration of storage is made regular check on, in time the abnormal fruit of cleaning.
Preferably: described Grape During Storage condition is relative humidity 85-95%, temperature is 2 ± 0.5 DEG C.
Beneficial effect of the present invention is: the present invention extracts bio-preservative with honeysuckle raw material and carries out fresh-keeping to grape, safety.Nontoxic, nuisanceless, fresh-keeping for grape of chemical bactericide, anticorrisive agent can be replaced, avoid the problem such as pollution, food security of chemical bactericide to environment.
Detailed description of the invention
Below in conjunction with detailed description of the invention, the present invention will be further described.
Embodiment 1
From honeysuckle, extract a method for bio-preservative, comprise the steps, 1, honeysuckle is cleaned, removing leaf, branch and other foreign material; 2, pulverizer is adopted to pulverize honeysuckle; 2, be that the ratio of 1:15 adds ethanolic solution with solid-liquid ratio by the honeysuckle of pulverizing, shake up, and be transferred to together in Microwave Extraction Apparatus and carry out Microwave Extraction, Extracting temperature is 65 DEG C, and the working time is 5min, and microwave power is 500w; 3, by extracting liquid filtering, filtrate is obtained, and in 2 DEG C of storages.Wherein, ethanol solution concentration is 50%.Add rotor in leaching process to stir, contribute to the extraction of bio-preservative.
The present invention also provides a kind of application of bio-preservative in grape fresh-keeping of extracting according to method described in claim 1, immerses 5min in bio-preservative, dry, then preserve with after PE-PP composite waterproofing membrane after the grape newly plucked being rejected insect pest and mechanical damage fruit; Duration of storage is made regular check on, in time the abnormal fruit of cleaning.Grape During Storage condition is relative humidity 90%, and temperature is 1.5 DEG C.
Embodiment 2
From honeysuckle, extract a method for bio-preservative, comprise the steps, 1, honeysuckle is cleaned, removing leaf, branch and other foreign material; 2, pulverizer is adopted to pulverize honeysuckle; 3, be that the ratio of 1:20 adds ethanolic solution with solid-liquid ratio by the honeysuckle of pulverizing, and be transferred to together in Microwave Extraction Apparatus and carry out Microwave Extraction, Extracting temperature is 75 DEG C, and the working time is 10min, and microwave power is 700w; 3, by extracting liquid filtering, filtrate is obtained, and in 4 DEG C of storages.Wherein, ethanol solution concentration is 60%.Add rotor in leaching process to stir, contribute to the extraction of bio-preservative.
The present invention also provides a kind of application of bio-preservative in grape fresh-keeping of extracting according to method described in claim 1, immerses 5min in bio-preservative, dry, then preserve with after PE-PP composite waterproofing membrane after the grape newly plucked being rejected insect pest and mechanical damage fruit; Duration of storage is made regular check on, in time the abnormal fruit of cleaning.Grape During Storage condition is relative humidity 95%, and temperature is 2.5 DEG C.
Embodiment 3
From honeysuckle, extract a method for bio-preservative, comprise the steps, 1, honeysuckle is cleaned, removing leaf, branch and other foreign material; 2, pulverizer is adopted to pulverize honeysuckle; 3, be that the ratio of 1:17 adds ethanolic solution with solid-liquid ratio by the honeysuckle of pulverizing, and be transferred to together in Microwave Extraction Apparatus and carry out Microwave Extraction, Extracting temperature is 70 DEG C, and the working time is 7min, and microwave power is 600w; 4, by extracting liquid filtering, filtrate is obtained, and in 3 DEG C of storages.Wherein, ethanol solution concentration is 55%.Add rotor in leaching process to stir, contribute to the extraction of bio-preservative.
The present invention also provides a kind of application of bio-preservative in grape fresh-keeping of extracting according to method described in claim 1, immerses 4min in bio-preservative, dry, then preserve with after PE-PP composite waterproofing membrane after the grape newly plucked being rejected insect pest and mechanical damage fruit; Duration of storage is made regular check on, in time the abnormal fruit of cleaning.Grape During Storage condition is relative humidity 90%, and temperature is 2 DEG C.
Embodiment 4
From honeysuckle, extract a method for bio-preservative, comprise the steps, 1, honeysuckle is cleaned, removing leaf, branch and other foreign material; 2, pulverizer is adopted to pulverize honeysuckle; 3, be that the ratio of 1:18 adds ethanolic solution with solid-liquid ratio by the honeysuckle of pulverizing, and be transferred to together in Microwave Extraction Apparatus and carry out Microwave Extraction, Extracting temperature is 72 DEG C, and the working time is 9min, and microwave power is 580w; 4, by extracting liquid filtering, filtrate is obtained, and in 2 DEG C of storages.Wherein, ethanol solution concentration is 55%.Add rotor in leaching process to stir, contribute to the extraction of bio-preservative.
The present invention also provides a kind of application of bio-preservative in grape fresh-keeping of extracting according to method described in claim 1, immerses 5min in bio-preservative, dry, then preserve with after PE-PP composite waterproofing membrane after the grape newly plucked being rejected insect pest and mechanical damage fruit; Duration of storage is made regular check on, in time the abnormal fruit of cleaning.Grape During Storage condition is relative humidity 90%, and temperature is 2 DEG C.
Claims (5)
1. from honeysuckle, extract a method for bio-preservative, it is characterized in that: comprise the steps,
(1) honeysuckle is cleaned, removing leaf, branch and other foreign material;
(2) pulverizer is adopted to pulverize honeysuckle;
(3) be that the ratio of 1:15-20 adds ethanolic solution with solid-liquid ratio by the honeysuckle of pulverizing, shake up, and be transferred to together in Microwave Extraction Apparatus and carry out Microwave Extraction, Extracting temperature is 65-75 DEG C, and the working time is 5-10min, and microwave power is 500-700w;
(4) by extracting liquid filtering, filtrate is obtained, and in 2-4 DEG C of storage.
2. according to the method extracting bio-preservative from honeysuckle according to claim 1, it is characterized in that: described ethanol solution concentration is 50-60%.
3. according to the method extracting bio-preservative from honeysuckle according to claim 1, it is characterized in that: add rotor in described leaching process and stir.
4. the application of bio-preservative in grape fresh-keeping of extracting according to method described in claim 1, it is characterized in that: after the grape newly plucked being rejected insect pest and mechanical damage fruit, immerse 5min in bio-preservative, dry, then preserve with after PE-PP composite waterproofing membrane; Duration of storage is made regular check on, in time the abnormal fruit of cleaning.
5. according to the application of bio-preservative according to claim 4 in grape fresh-keeping, it is characterized in that: described Grape During Storage condition is relative humidity 85-95%, temperature is 2 ± 0.5 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310610624.0A CN104663858A (en) | 2013-11-26 | 2013-11-26 | Method for extracting biological fresh-keeping agent from honeysuckle, and applications of biological fresh-keeping agent in grape fresh keeping |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310610624.0A CN104663858A (en) | 2013-11-26 | 2013-11-26 | Method for extracting biological fresh-keeping agent from honeysuckle, and applications of biological fresh-keeping agent in grape fresh keeping |
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| CN104663858A true CN104663858A (en) | 2015-06-03 |
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| CN201310610624.0A Pending CN104663858A (en) | 2013-11-26 | 2013-11-26 | Method for extracting biological fresh-keeping agent from honeysuckle, and applications of biological fresh-keeping agent in grape fresh keeping |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104904840A (en) * | 2015-06-24 | 2015-09-16 | 南京大地冷冻食品有限公司 | Cucumber and honeysuckle flower coating preservation solution |
| CN104996554A (en) * | 2015-06-24 | 2015-10-28 | 南京大地冷冻食品有限公司 | Application of cucumber and honeysuckle coating preservation liquid in freshness preservation of fruits, vegetables and chilled meat |
-
2013
- 2013-11-26 CN CN201310610624.0A patent/CN104663858A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104904840A (en) * | 2015-06-24 | 2015-09-16 | 南京大地冷冻食品有限公司 | Cucumber and honeysuckle flower coating preservation solution |
| CN104996554A (en) * | 2015-06-24 | 2015-10-28 | 南京大地冷冻食品有限公司 | Application of cucumber and honeysuckle coating preservation liquid in freshness preservation of fruits, vegetables and chilled meat |
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Application publication date: 20150603 |