CN104663856A - Method for extracting biological fresh keeping agent from honeysuckle flower, and application of biological fresh keeping agent in fresh keeping of hawthorn fruits - Google Patents
Method for extracting biological fresh keeping agent from honeysuckle flower, and application of biological fresh keeping agent in fresh keeping of hawthorn fruits Download PDFInfo
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- CN104663856A CN104663856A CN201310608869.XA CN201310608869A CN104663856A CN 104663856 A CN104663856 A CN 104663856A CN 201310608869 A CN201310608869 A CN 201310608869A CN 104663856 A CN104663856 A CN 104663856A
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- honeysuckle
- fresh keeping
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Abstract
The invention relates to a method for extracting a biological fresh keeping agent from honeysuckle flower. The method comprises the following steps: 1, cleaning honeysuckle flower to remove leaves, branches and other foreign materials; 2, crushing the honeysuckle flower by adopting a crusher to 50-100 meshes; 3, adding the crushed honeysuckle flower to an ethanol solution according to a solid/liquid ratio of 1:3-5, shaking up, transferring the obtained mixture to a microwave extraction instrument, and carrying out microwave extraction at 60-75DEG C under a microwave power 500-700w for 5-10min; and 4, filtering the obtained extract liquid to obtain a filtrate, and storing the filtrate at 2-4DEG C. The invention also provides an application of the extracted biological fresh keeping agent in the fresh keeping of the hawthorn fruits. The biological fresh keeping agent extracted from the honeysuckle flower is safe, nontoxic and nuisanceless in the fresh keeping of the hawthorn fruits, can be used in the fresh keeping of hawthorn fruits to replace chemical bactericides and antiseptics, and avoids the pollution of the chemical bactericides to environment and food safety.
Description
Technical field
The present invention relates to the field of preserving fruit and vegetable utilizing, the bio-preservative extracted from honeysuckle specifically and the application in hawthorn is fresh-keeping thereof.
Background technology
Honeysuckle, has another name called two flowers, and be dry flower or the flower just opened of Caprifoliaceae woodbine honeysuckle, in China, most area all has production, is China's traditional Chinese medicine material, taste is sweet, cold in nature, clearing heat and detoxicating, wind-heat dissipating.Modern chemical analysis and pharmacological evaluation prove, honeysuckle have broad-spectrum antiseptic, antiviral, antitumor, strengthen immunity and antipyretic and anti-inflammatory, cholagogic, protect the liver, lipopenicillinase, antifertility, hemostasis, the pharmacological action such as antiulcer.It is reported, the whole nation has the bud of more than 20 Plants to be used as medicine when honeysuckle, is all Caprifoliaceae woodbine.Owing to having special drug effect, therefore become the study hotspot of Chinese herbal medicine in recent years.Containing cyanidenon in honeysuckle, isochlorogenic acid, the multiple compounds such as chlorogenic acid and flavones, tradition thinks that chlorogenic acid is the principle active component of honeysuckle, but show according to modern medicine study, flavone compound wherein has reduction MCO, make coronary artery, cerebrovascular flow increases, the anti-rhythm of the heart, softening blood vessel, hypoglycemic, the effects such as blood fat, simultaneously it or a kind of natural antioxidant, there is Superoxide anion free radical in removing human body anti-ageing, increase the physiological activity of immunity of organisms, therefore its biologically active becomes the focus of people's research day by day.
Haw flakes are containing multivitamin, crataegolic acid, tartaric acid, citric acid, malic acid etc., and also containing the mineral matter such as flavonoids, lactone, carbohydrate, protein, fat and calcium, phosphorus, iron, contained lipolytic enzyme can promote the digestion of fat food.Promote gastric secretion and increase the functions such as stomach endoenzyme element.The traditional Chinese medical science is thought, hawthorn has the effects such as long-pendingization that disappear is stagnant, convergence stops dysentery, activate blood circulation and disperse blood clots.Cure mainly the diseases such as eating accumulation, chest diaphragm spleen are full, the amenorrhoea of hernia blood stasis.Containing the drug ingedient such as mountain terpene and flavonoids in hawthorn, there is significant hemangiectasis and hypotensive effect, have the function strengthening cardiac muscle, arrhythmia, adjusting blood lipid and cholesterol level.Accommodation: common people are edible all.Children, the elderly, indigestion especially edibility.Cold, indigestion, poor appetite, children's cartilage acalcicosis, Iron deficiency anemia in children person can eat haw flakes more.
Effect of hawthorn and effect: 1, prevention and cure of cardiovascular disease, reduce blood pressure and cholesterol, softening blood vessel and diuresis and sedation; 2, appetite-stimulating indigestion-relieving, offsets carnivorous stagnant effect especially better, all have employed hawthorn in a lot of digestant medicine; 3, hawthorn has the effect activated blood circulation and dispersed blood clots, and contributes to removing local extravasated blood state.
Hawthorn, delicious flavour, rich in nutritive value, but the respiratory intensity of hawthorn is large, and protective tissue is poor, and preservation and freshness is more difficult, and preservation term and shelf life are all shorter, can bring great loss like this for orchard worker and consumer.And the preservation method of current conventional fruit mainly adopts chemical bactericide, and a large amount of uses of chemical bactericide, pathogenic microorganism can be caused to produce drug resistance, and environmental pollution; In addition, because hawthorn cleaning is more difficult, be difficult to thoroughly wash away chemical bactericide, after edible, health hidden danger can be caused.
Summary of the invention
According to above-mentioned weak point, the object of this invention is to provide a kind of with the honeysuckle extraction method of bio-preservative and the application in hawthorn is fresh-keeping thereof.
For achieving the above object, technical scheme of the present invention is: a kind of method extracting bio-preservative from honeysuckle, comprises the steps, 1, honeysuckle is cleaned, and removing leaf, branch and other foreign material; 2, pulverizer is adopted by honeysuckle to be crushed to 50-100 order; 3, be that the ratio of 1:3-5 adds ethanolic solution with solid-liquid ratio by the honeysuckle of pulverizing, shake up, and be transferred to together in Microwave Extraction Apparatus and carry out Microwave Extraction, Extracting temperature is 60-75 DEG C, and the working time is 5-10min, and microwave power is 500-700w; 4, by extracting liquid filtering, filtrate is obtained, and in 2-4 DEG C of storage.
Preferably: described ethanol solution concentration is 70-75%.
Preferably: add rotor in described leaching process and stir.
The present invention also provides a kind of application of bio-preservative in hawthorn is fresh-keeping of extracting according to method described in claim 1, immerses 5min in bio-preservative, dry, then preserve with after PE-PP composite waterproofing membrane after the hawthorn of newly plucking being rejected insect pest and mechanical damage fruit; Duration of storage is made regular check on, in time the abnormal fruit of cleaning.
Preferably: described hawthorn storage condition is relative humidity 85-95%, temperature is 2 ± 0.5 DEG C.
Beneficial effect of the present invention is: the present invention is that raw material extraction bio-preservative carries out fresh-keeping to hawthorn with honeysuckle, safety.Nontoxic, nuisanceless, fresh-keeping for hawthorn of chemical bactericide, anticorrisive agent can be replaced, avoid the problem such as pollution, food security of chemical bactericide to environment.
Detailed description of the invention
Below in conjunction with detailed description of the invention, the present invention will be further described.
Embodiment 1
From honeysuckle, extract a method for bio-preservative, comprise the steps, 1, honeysuckle is cleaned, removing leaf, branch and other foreign material; 2, pulverizer is adopted by honeysuckle to be crushed to 50 orders; 2, be that the ratio of 1:3 adds ethanolic solution with solid-liquid ratio by the honeysuckle of pulverizing, shake up, and be transferred to together in Microwave Extraction Apparatus and carry out Microwave Extraction, Extracting temperature is 60 DEG C, and the working time is 5min, and microwave power is 500w; 3, by extracting liquid filtering, filtrate is obtained, and in 2 DEG C of storages.Wherein, ethanol solution concentration is 70%.Add rotor in leaching process to stir, contribute to the extraction of bio-preservative.
The present invention also provides a kind of application of bio-preservative in hawthorn is fresh-keeping of extracting according to method described in claim 1, immerses 5min in bio-preservative, dry, then preserve with after PE-PP composite waterproofing membrane after the hawthorn of newly plucking being rejected insect pest and mechanical damage fruit; Duration of storage is made regular check on, in time the abnormal fruit of cleaning.Hawthorn storage condition is relative humidity 85%, and temperature is 1.5 DEG C.
Embodiment 2
From honeysuckle, extract a method for bio-preservative, comprise the steps, 1, honeysuckle is cleaned, removing leaf, branch and other foreign material; 2, pulverizer is adopted by honeysuckle to be crushed to 100 orders; 3, be that the ratio of 1:5 adds ethanolic solution with solid-liquid ratio by the honeysuckle of pulverizing, and be transferred to together in Microwave Extraction Apparatus and carry out Microwave Extraction, Extracting temperature is 75 DEG C, and the working time is 10min, and microwave power is 700w; 3, by extracting liquid filtering, filtrate is obtained, and in 4 DEG C of storages.Wherein, ethanol solution concentration is 75%.Add rotor in leaching process to stir, contribute to the extraction of bio-preservative.
The present invention also provides a kind of application of bio-preservative in hawthorn is fresh-keeping of extracting according to method described in claim 1, immerses 5min in bio-preservative, dry, then preserve with after PE-PP composite waterproofing membrane after the hawthorn of newly plucking being rejected insect pest and mechanical damage fruit; Duration of storage is made regular check on, in time the abnormal fruit of cleaning.Hawthorn storage condition is relative humidity 95%, and temperature is 2.5 DEG C.
Embodiment 3
From honeysuckle, extract a method for bio-preservative, comprise the steps, 1, honeysuckle is cleaned, removing leaf, branch and other foreign material; 2, pulverizer is adopted to be crushed to 70 orders honeysuckle; 3, be that the ratio of 1:4 adds ethanolic solution with solid-liquid ratio by the honeysuckle of pulverizing, and be transferred to together in Microwave Extraction Apparatus and carry out Microwave Extraction, Extracting temperature is 70 DEG C, and the working time is 7min, and microwave power is 600w; 4, by extracting liquid filtering, filtrate is obtained, and in 3 DEG C of storages.Wherein, ethanol solution concentration is 73%.Add rotor in leaching process to stir, contribute to the extraction of bio-preservative.
The present invention also provides a kind of application of bio-preservative in hawthorn is fresh-keeping of extracting according to method described in claim 1, immerses 5min in bio-preservative, dry, then preserve with after PE-PP composite waterproofing membrane after the hawthorn of newly plucking being rejected insect pest and mechanical damage fruit; Duration of storage is made regular check on, in time the abnormal fruit of cleaning.Hawthorn storage condition is relative humidity 90%, and temperature is 2 DEG C.
Embodiment 4
From honeysuckle, extract a method for bio-preservative, comprise the steps, 1, honeysuckle is cleaned, removing leaf, branch and other foreign material; 2, pulverizer is adopted by honeysuckle to be crushed to 90 orders; 3, be that the ratio of 1:4 adds ethanolic solution with solid-liquid ratio by the honeysuckle of pulverizing, and be transferred to together in Microwave Extraction Apparatus and carry out Microwave Extraction, Extracting temperature is 72 DEG C, and the working time is 9min, and microwave power is 670w; 4, by extracting liquid filtering, filtrate is obtained, and in 2 DEG C of storages.Wherein, ethanol solution concentration is 74%.Add rotor in leaching process to stir, contribute to the extraction of bio-preservative.
The present invention also provides a kind of application of bio-preservative in hawthorn is fresh-keeping of extracting according to method described in claim 1, immerses 5min in bio-preservative, dry, then preserve with after PE-PP composite waterproofing membrane after the hawthorn of newly plucking being rejected insect pest and mechanical damage fruit; Duration of storage is made regular check on, in time the abnormal fruit of cleaning.Hawthorn storage condition is relative humidity 90%, and temperature is 2 DEG C.
Claims (5)
1. from honeysuckle, extract a method for bio-preservative, it is characterized in that: comprise the steps,
(1) honeysuckle is cleaned, removing leaf, branch and other foreign material;
(2) pulverizer is adopted by honeysuckle to be crushed to 50-100 order;
(3) be that the ratio of 1:3-5 adds ethanolic solution with solid-liquid ratio by the honeysuckle of pulverizing, shake up, and be transferred to together in Microwave Extraction Apparatus and carry out Microwave Extraction, Extracting temperature is 60-75 DEG C, and the working time is 5-10min, and microwave power is 500-700w;
(4) by extracting liquid filtering, filtrate is obtained, and in 2-4 DEG C of storage.
2. according to the method extracting bio-preservative from honeysuckle according to claim 1, it is characterized in that: described ethanol solution concentration is 70-75%.
3. according to the method extracting bio-preservative from honeysuckle according to claim 1, it is characterized in that: add rotor in described leaching process and stir.
4. the application of bio-preservative in hawthorn is fresh-keeping of extracting according to method described in claim 1, it is characterized in that: after the hawthorn of newly plucking being rejected insect pest and mechanical damage fruit, immerse 5min in bio-preservative, dry, then preserve with after PE-PP composite waterproofing membrane; Duration of storage is made regular check on, in time the abnormal fruit of cleaning.
5. according to the application of bio-preservative according to claim 4 in hawthorn is fresh-keeping, it is characterized in that: described hawthorn storage condition is relative humidity 85-95%, temperature is 2 ± 0.5 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310608869.XA CN104663856A (en) | 2013-11-26 | 2013-11-26 | Method for extracting biological fresh keeping agent from honeysuckle flower, and application of biological fresh keeping agent in fresh keeping of hawthorn fruits |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310608869.XA CN104663856A (en) | 2013-11-26 | 2013-11-26 | Method for extracting biological fresh keeping agent from honeysuckle flower, and application of biological fresh keeping agent in fresh keeping of hawthorn fruits |
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| CN104663856A true CN104663856A (en) | 2015-06-03 |
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| CN201310608869.XA Pending CN104663856A (en) | 2013-11-26 | 2013-11-26 | Method for extracting biological fresh keeping agent from honeysuckle flower, and application of biological fresh keeping agent in fresh keeping of hawthorn fruits |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105851218A (en) * | 2016-05-04 | 2016-08-17 | 四川中海生物科技开发股份有限公司 | Biological fresh-keeping agent extracted from idesia polycarpa and preparation and application thereof |
| CN106578016A (en) * | 2016-12-06 | 2017-04-26 | 江南大学 | Method for extracting biological preservative from peony ball and application of biological preservative |
-
2013
- 2013-11-26 CN CN201310608869.XA patent/CN104663856A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105851218A (en) * | 2016-05-04 | 2016-08-17 | 四川中海生物科技开发股份有限公司 | Biological fresh-keeping agent extracted from idesia polycarpa and preparation and application thereof |
| CN105851218B (en) * | 2016-05-04 | 2020-08-11 | 四川中海茂农业开发有限公司 | Biological preservative extracted from idesia polycarpa and preparation and application thereof |
| CN106578016A (en) * | 2016-12-06 | 2017-04-26 | 江南大学 | Method for extracting biological preservative from peony ball and application of biological preservative |
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Application publication date: 20150603 |