CN103289809B - Method for preparing walnut seed kernel oil by adopting brine enzymatic method - Google Patents
Method for preparing walnut seed kernel oil by adopting brine enzymatic method Download PDFInfo
- Publication number
- CN103289809B CN103289809B CN201310208512.2A CN201310208512A CN103289809B CN 103289809 B CN103289809 B CN 103289809B CN 201310208512 A CN201310208512 A CN 201310208512A CN 103289809 B CN103289809 B CN 103289809B
- Authority
- CN
- China
- Prior art keywords
- oil
- seed kernel
- walnut kernel
- kernel oil
- walnut seed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Fats And Perfumes (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Preparation Of Fruits And Vegetables (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides a method for preparing walnut seed kernel oil by adopting a brine enzymatic method. The method comprises the following steps of: firstly, drying walnut seed kernel at 40-60 DEG C, grinding the dried walnut seed kernel into pulp by a pulping machine, adding sodium chloride, alpha-amylase, cellulose and distilled water into the pulp, and evenly stirring and mixing; carrying out enzymolysis for 2-4h at 50-60 DEG C by stirring to obtain an enzymoiysis product; finally, carrying out centrifugation on the enzymoiysis product to obtain a centrifugal product which is the pure natural walnut seed kernel oil. After being analyzed by a gas chromatography-mass spectrometer (GC-MS), the walnut seed kernel oil prepared by the method contains rich unsaturated fatty acid, is high in oil quality, and has rich nutritive value, thus being high-quality edible oil and having functions of medical treatment and health care; furthermore, the enzymoiysis is carried out by compounding enzyme and the sodium chloride, so that the walnut seed kernel oil is low in dosage of enzyme, low in extraction cost and high in extraction ratio (79-82%) of oil product.
Description
Technical field
The present invention relates to a kind of preparation method of walnut kernel oil, relate in particular to a kind of method that salt aqueous enzymatic method is prepared walnut kernel oil, belong to vegetables oil processing technique field.
Background technology
Walnut (Juglans) belongs to Angiospermae Dicotyledoneae Juglandaceae (Juglanadaceae), cries again English walnut, originates in the area such as W. Asia, West Asia, is one of ancient fruit tree kind that China's cultivation history is long, germ plasm resource is abundant.Walnut is important nut and woody oil tree species, there is very high economic worth: walnut kernel percent is up to 50% left and right, and it is nutritious and delicious, can eat raw, also be the high quality raw material of making cake, be listed as the large dry fruit in the world four with almond (comet assay), cashew nut, ear of maize.Walnut has fine health-care effect simultaneously, and China people claim that it is " long live ", " Longevity ", and it is " strong man's food " or " nutritious nut " person abroad.Often eat that walnut can not only nourish blood vessels, improves a poor appetite, pitch-black beard and hair, and can also cure sexual hypofunction, weak, amnesia etc., have saying of " often eating walnut, recover one's youthful vigour " so among the people.Walnut kernel oleaginousness reaches 60% ~ 70%, and wherein unsaturated fatty acid content is more than 90%, also higher than the oleaginousness of soybean, Semen Brassicae campestris, peanut and sesame, have the title of " woody oleiferous plants king ".In addition, in walnut oil, also contain the compositions such as linolic acid that human body can not be synthetic and linolenic acid, oleic acid, and multiple to the useful VITAMIN of human body and mineral element, as flavonoid, VE and iron etc., therefore, walnut oil is nutritious, and delicious delicate fragrance also, kept nutritive health-care and the pharmacological effect of walnut kernel, therefore walnut oil is a kind of nutritional oil of preciousness.
CN200910011719.4 provides a kind of preparation method of walnut oil, is through squeezing, filtration, winterization, the process such as squeezing and obtaining repeatedly by walnut kernel.Because the consumption of squeezing required drive is large, high to equipment requirement of strength, extraction yield is lower.CN201010579440.9 provides a kind of working method of walnut oil, be by walnut kernel through squeezing, atomization decon, come unstuck, decolouring, deodorization, filtration, obtain walnut oil.Although the method obtains the walnut oil of higher quality, complex technical process, cost is higher.The technique that CN201210561556.9 provides a kind of water substitution to produce walnut oil is by the baking of stoning peach kernel, fragmentation.Mill sauce, convert slurry, calmly the technique such as slurry, oil skimming completes.The subject matter that the method exists is that extraction yield is low, and power consumption is large, when baking, the quality of oil product is caused to very large impact.
Summary of the invention
The object of the invention is for problems of the prior art, provide a kind of salt aqueous enzymatic method to prepare the method for walnut kernel oil.
Salt aqueous enzymatic method of the present invention is prepared the method for walnut kernel oil, be by dry to walnut kernel and 40-60 DEG C, after paste roller mill is worn into slip, in its slurry, add sodium-chlor, α-amylase, cellulase and distilled water, making the solid-liquid ratio of mixed system is 1:4 ~ 1:6g/mL, is uniformly mixed; At 50 DEG C ~ 60 DEG C, stir enzymolysis 2 ~ 4h, obtain enzymolysis product; Finally by enzymolysis product in centrifugal, centrifugal product, obtains pure natural walnut kernel oil.
The add-on of described sodium-chlor is 8% ~ 12% of walnut kernel quality; Vigor >=3700 unit of activity/the g of α-amylase, the addition of α-amylase are 0.6% ~ 1% of walnut kernel quality; The vigor of cellulase is 10000u/g, and its addition is 8% ~ 12% of walnut kernel quality.
Described centrifugal be centrifugal 15 ~ 30min under the rotating speed of the temperature of 15 ~ 25 DEG C, 6000 ~ 8000r/min.
After measured, the extraction rate reached that the inventive method is prepared walnut kernel oil is 79% ~ 82%.
Walnut kernel oil prepared by the present invention utilizes GC-MS to analyze lipid acid and content in walnut kernel oil:
Esterification method: adopt the ester-interchange method in GB/T17376-2008;
GC conditions: GC condition: (30 mm × 0.25, m × 0.25 μ m) for RTX-5MS type elastic quartz capillary tube; Carrier gas He, flow velocity 1.0mL/min; Splitting ratio is 50:1.Transmission line temperature is 250 DEG C; 250 DEG C of injector temperatures.Temperature programming: 160 DEG C of maintenance 3min of initial temperature; Be elevated to 210 DEG C, reservation 1 min with 2 DEG C/min from 160 DEG C; Be elevated to 250 DEG C with 5 DEG C/min from 210 DEG C, keep 1 min, sample size is 1.0 μ L;
Mass spectrum condition: 250 DEG C of ion source temperatures, electron energy 70 eV, sweep limit 50 ~ 650 amu.
Gas-chromatography is shown in Fig. 1.In the walnut kernel oil that GC-MS measures, lipid acid and content are in table 1.The result demonstration of Fig. 1 and table 1, in gained walnut oil of the present invention, unsaturated fatty acids reaches more than 98%, and nutritive ingredient is very abundant.
Fatty acid component and the content of table 1 gained walnut oil of the present invention
In sum, the following beneficial effect of the relative prior art of the present invention:
1, in the Semen Juglandis oil that prepared by the present invention, contain abundant unsaturated fatty acids, oil quality is high, has extremely abundant nutritive value, is the edible oil of high-quality, has medical treatment and health-care effect simultaneously;
2, the present invention adopts the composite enzymolysis that carries out of enzyme and sodium-chlor, and the consumption of enzyme is few, and extraction cost is low, and the extraction yield of oil product is high.
3, to prepare the technique of Semen Juglandis oil simple in the present invention, and power consumption is little, and environment-protecting clean can be produced in industrialization.
Brief description of the drawings
Fig. 1 is gained walnut of the present invention oil composition and mol ratio gas-chromatography.
Embodiment
Below by specific embodiment, the preparation method of walnut kernel of the present invention is described further.
embodiment 1
(1) take the walnut kernel (oleaginousness is 60% ~ 70%) of 20g, after 40 DEG C of left and right are dry, wear into slip through sesame oil paste roller mill;
(2) in slip, add the sodium-chlor of 1.6g, (vigor >=3700 unit of activity/g), 2.0g cellulase (vigor 10000u/g), then adds 100mL adding distil water to 0.16g α-amylase, is uniformly mixed;
(3) mixed system is stirred at 50 DEG C to enzymolysis 2h, obtain enzymolysis product;
(4) by enzymolysis product centrifugal 15min under 15 DEG C of temperature, rotating speed 6000r/min; Collect the centrifugal product in upper strata, be pure natural walnut kernel oil, extraction yield is 78.93%.
embodiment 2
(1) take the walnut kernel (oleaginousness is 60% ~ 70%) of 20g, after 50 DEG C of left and right are dry, wear into slip through sesame oil paste roller mill;
(2) in slip, add the sodium-chlor of 2.0g, (vigor >=3700 unit of activity/g), 2.4g cellulase (vigor 10000u/g), then adds 120mL adding distil water to 0.2g α-amylase, is uniformly mixed;
(3) mixed system is stirred at 55 DEG C to enzymolysis 3h, obtain enzymolysis product;
(4) by enzymolysis product centrifugal 20min under 20 DEG C of temperature, rotating speed 7000r/min; Collect the centrifugal product in upper strata, be pure natural walnut kernel oil, extraction yield is 80.35%.
embodiment 3
(1) take the walnut kernel (oleaginousness is 60% ~ 70%) of 20g, after 60 DEG C of left and right are dry, wear into slip through sesame oil paste roller mill;
(2) in slip, add the sodium-chlor of 2.4g, (vigor >=3700 unit of activity/g), 2.8g cellulase (vigor 10000u/g), then adds 140 mL adding distil waters to 0.24g α-amylase, is uniformly mixed;
(3) mixed system is stirred at 60 DEG C to enzymolysis 4h, obtain enzymolysis product;
(4) by enzymolysis product centrifugal 30min under 25 DEG C of temperature, rotating speed 8000r/min; Collect the centrifugal product in upper strata, be pure natural walnut kernel oil, extraction yield is 79.06%.
Claims (3)
1. salt aqueous enzymatic method is prepared the method for walnut kernel oil, be first by walnut kernel in 40-60 DEG C dry, after paste roller mill is worn into slip, add wherein sodium-chlor, α-amylase, cellulase and distilled water, be uniformly mixed; Then at 50 DEG C～60 DEG C, stir enzymolysis 2～4h, obtain enzymolysis product; Finally that enzymolysis product is centrifugal, obtain pure natural walnut kernel oil; The add-on of described sodium-chlor is 8%～12% of walnut kernel quality; Vigor >=3700 unit of activity/the g of described α-amylase, the addition of α-amylase is 0.6%～1% of walnut kernel quality; The vigor of described cellulase is 10000u/g, and its addition is 8%～12% of walnut kernel quality.
2. salt aqueous enzymatic method is prepared the method for walnut kernel oil as claimed in claim 1, it is characterized in that: the solid-liquid ratio that adds sodium-chlor, α-amylase, cellulase and distilled water mixed system in slip is 1:4～1:6g/mL.
3. salt aqueous enzymatic method is prepared the method for walnut kernel oil as claimed in claim 1, it is characterized in that: described centrifugal be centrifugal 15～30min under the rotating speed of the temperature of 15～25 DEG C, 6000～8000r/min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310208512.2A CN103289809B (en) | 2013-05-30 | 2013-05-30 | Method for preparing walnut seed kernel oil by adopting brine enzymatic method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310208512.2A CN103289809B (en) | 2013-05-30 | 2013-05-30 | Method for preparing walnut seed kernel oil by adopting brine enzymatic method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103289809A CN103289809A (en) | 2013-09-11 |
| CN103289809B true CN103289809B (en) | 2014-07-23 |
Family
ID=49091352
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310208512.2A Active CN103289809B (en) | 2013-05-30 | 2013-05-30 | Method for preparing walnut seed kernel oil by adopting brine enzymatic method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103289809B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104450152B (en) * | 2013-09-23 | 2017-11-21 | 贵州周以晴生物科技有限公司 | A kind of combination process of extracting tea seed oil by using enzymatic hydrolysis |
| CN105565404A (en) * | 2015-12-25 | 2016-05-11 | 浙江农林大学 | Pecan oil aqueous enzymatic extraction method |
| CN108998213A (en) * | 2018-06-15 | 2018-12-14 | 临江市山花绿色食品有限公司 | A kind of preparation method of walnut oil |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1854273A (en) * | 2005-04-26 | 2006-11-01 | 中国科学院沈阳应用生态研究所 | Extraction of hickory nut oil from water enzyme |
| CN101816341A (en) * | 2010-04-27 | 2010-09-01 | 西北师范大学 | Method for extracting walnut oil through microwave-assisted enzymolysis |
| CN102080107A (en) * | 2010-12-13 | 2011-06-01 | 昆明理工大学 | Method for synchronously preparing walnut oil and walnut protein peptide by microwave-assisted protease hydrolysis |
-
2013
- 2013-05-30 CN CN201310208512.2A patent/CN103289809B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1854273A (en) * | 2005-04-26 | 2006-11-01 | 中国科学院沈阳应用生态研究所 | Extraction of hickory nut oil from water enzyme |
| CN101816341A (en) * | 2010-04-27 | 2010-09-01 | 西北师范大学 | Method for extracting walnut oil through microwave-assisted enzymolysis |
| CN102080107A (en) * | 2010-12-13 | 2011-06-01 | 昆明理工大学 | Method for synchronously preparing walnut oil and walnut protein peptide by microwave-assisted protease hydrolysis |
Non-Patent Citations (8)
| Title |
|---|
| 响应面法优化水酶法提取核桃油的工艺条件;徐效圣等;《食品与机械》;20100331;第26卷(第2期);第92-96页 * |
| 徐效圣等.响应面法优化水酶法提取核桃油的工艺条件.《食品与机械》.2010,第26卷(第2期),第92-96页. |
| 易建华等.水酶法提取核桃油工艺研究—酶解和离心参数.《中国油脂》.2007,第32卷(第4期),第20-22页. |
| 易建华等.酶的选择对水酶法提取核桃油的影响.《中国油脂》.2007,第32卷(第2期),第27-29页. |
| 朱振宝等.盐在水酶法提取核桃油中的影响及酶解条件优化.《食品科技》.2012,第37卷(第9期),第175-179页. |
| 水酶法提取核桃油工艺研究—酶解和离心参数;易建华等;《中国油脂》;20070430;第32卷(第4期);第20-22页 * |
| 盐在水酶法提取核桃油中的影响及酶解条件优化;朱振宝等;《食品科技》;20120930;第37卷(第9期);第178页右栏第3段 * |
| 酶的选择对水酶法提取核桃油的影响;易建华等;《中国油脂》;20070228;第32卷(第2期);第28页左栏1.3.3.2 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103289809A (en) | 2013-09-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101455240B (en) | Pumpkin seed oil extraction method using water enzyme method | |
| CN100427576C (en) | Extraction of hickory nut oil from water enzyme | |
| CN101812111A (en) | Comprehensive deep processing method for oil tea fruits | |
| CN105166888A (en) | Ginseng and pilose antler tea seed polypeptide lozenge and preparation method therefor | |
| CN105166939A (en) | Tea seed polypeptide lozenge and preparation method therefor | |
| CN104212629A (en) | Method for extracting linseed oil by cold pressing with salt-water wetting | |
| CN103815061A (en) | Blend edible oil of peony seed oil and sesame oil and preparation method of blend edible oil | |
| CN103421612A (en) | Method for manufacturing oil by using tea oil residues | |
| CN103289809B (en) | Method for preparing walnut seed kernel oil by adopting brine enzymatic method | |
| CN104745290A (en) | High-oil-yield sunflower seed oil extraction production process | |
| CN102504946B (en) | Method for refining camellia seed crude oil beneficial to people with high blood pressure, high blood sugar and high blood fat | |
| CN105132138A (en) | Processing technology for camellia seeds | |
| CN104862068A (en) | Process for preparing soybean oil by pressing method | |
| CN105886108A (en) | Pure camellia oil processing technology | |
| CN104045685A (en) | Method for extracting proteins by using camellia seed cakes | |
| CN103045345B (en) | Production method for preparing highly unsaturated active rapeseed oil by biological enzyme method | |
| CN107307372A (en) | A kind of fibert sauce and preparation method thereof | |
| CN203269905U (en) | Equipment for producing edible peony seed oil without adding preservatives | |
| CN105524708A (en) | Preparation method of hazelnut oil | |
| CN107183733B (en) | Method for preparing capsicum seed oil and capsicum seed dietary fiber | |
| CN105124575A (en) | Stomach strengthening and digestion helping tea seed polypeptide buccal tablet and preparation method thereof | |
| CN105670767A (en) | Method using camellia oleifera seed cake for oil expression | |
| CN105029448A (en) | Chinese yam and medlar kidney nourishing and body strengthening tea seed polypeptide lozenge and preparing method of lozenge | |
| CN104877755A (en) | Processing method for high-quality soybean oil | |
| CN105886103A (en) | Linolenic acid health care edible oil, Chinese prickly ash seed meal and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20180604 Address after: 043700 the new town of Yuanqu County, Shanxi Province, Zuo Jia Wan Village Patentee after: Yuanqu County Shun Tu original flavor agricultural and sideline products development Co., Ltd. Address before: 730070 No. 967 Ning Ning Road, Anning District, Lanzhou, Gansu Patentee before: Northwest Normal University |
|
| TR01 | Transfer of patent right |