CN103045345B

CN103045345B - Production method for preparing highly unsaturated active rapeseed oil by biological enzyme method

Info

Publication number: CN103045345B
Application number: CN201210220021.5A
Authority: CN
Inventors: 李春阳; 李跃; 张红城; 王帆
Original assignee: Jiangsu Academy of Agricultural Sciences
Current assignee: Jiangsu Academy of Agricultural Sciences
Priority date: 2012-06-29
Filing date: 2012-06-29
Publication date: 2014-05-07
Anticipated expiration: 2032-06-29
Also published as: CN103045345A

Abstract

The invention develops a method for preparing highly unsaturated active rapeseed oil by a biological enzyme method and belongs to the technical field of food processing. The production method comprises the following processes: peeling rapeseeds, inactivating enzyme in boiling water, grinding, carrying out composite enzymatic hydrolysis and breaking wall (NCB-PE40, Peetinex Ultra SP-L, Viscozyme L), alkaline extraction, protease hydrolysis (2.4L of Alcalase) and centrifugally separating to obtain the highly unsaturated active rapeseed oil. The method for preparing the highly unsaturated active rapeseed oil by the biological enzyme method has the characteristics that firstly, through the biological enzyme method to break the wall, the release of the rapeseed oil is promoted, and the oil output is greater than 95%; secondly, through proper protease enzymatic hydrolysis and the centrifugal separating (at 2000-5000rpm), the rapeseed oil is obtained, and the clean oil rate is higher than 60%; and thirdly, in comparison with the conventional pressing and extracting methods, the method disclosed by the invention is simple and easy to implement, and green and environment-friendly; the product is free from any pollution and solvent residue; the produced rapeseed oil contains more than 85% of unsaturated fatty acid, and is high in nutrition value and strong in activity.

Description

The production method of the high unsaturated active rapeseed oil of a kind of biological enzyme preparation

One, technical field

The production method that the invention provides the high unsaturated active rapeseed oil of a kind of biological enzyme preparation, belongs to food processing technology field.

Two, background technology

Rape is crop in cruciferae, and 4.13 hundred million mu of world's cultivated areas, 5,100 ten thousand tons of output, occupy oil crops second.1.16 hundred million mu of China's rapeseed cultivation areas, 1,100 ten thousand tons of output, account for respectively 24% and 25% of the world, is listed as first of the world.Semen Brassicae campestris is the important oil crops of China, accounts for 50% of domestic oil crops, and rapeseed oil consumption accounts for the more than 35% of domestic edible vegetable oil, occupies critical role at home on edible oil market.

Industry is prepared rapeseed oil and is mainly taked milling process and lixiviation process.Milling process, mainly by mechanical external force, squeezes out grease from oil plant, and product is not containing solvent, but its grouts resid amount is high, and oil yield efficiency is lower, and power consumption is large; Lixiviation process is selected special organic solvent, oil and grease extracting, and its productivity is high, oil yield is high, easily realizes scale production, but solvent for use is mostly inflammable and explosive, and production security is poor, finished product rapeseed oil has certain dissolvent residual, leach crude oil more containing non-oil substances, color and luster is darker, second-rate.Aqueous enzymatic method (claiming again water extraction) is emerging in recent years oil extracting process, utilizes enzymolysis broken wall, and by the effect of water, by centrifugal, edible vegetable oil is separated, and edible vegetable oil quality is high, security good.

The present invention adopts biological enzyme to produce rapeseed oil, by compound wall breaking enzyme, the release rate of oil in vegetable seed is reached more than 95%, then by proteolysis, centrifugal, obtains more than 60% edible vegetable oil, and its unsaturated fatty acid content reaches more than 85%.Present method can significantly reduce production costs, and improves production security, can not produce detrimentally affect to environment, meets the demand of human consumer to nutritive health-care grease, is conducive to the development of China's grease process deeply industry.

Three, summary of the invention

The present invention has developed a kind of method that biological enzyme is prepared high unsaturated active rapeseed oil.Take Semen Brassicae campestris as raw material, through arrangement, removal of impurities, peel, go out enzyme, defibrination, obtain vegetable seed slurry; By biological enzyme (polygalacturonase NCB-PE40, Pectinex Ultra SP-L, Viscozyme L) compound broken wall and protease A lcalase2.4L hydrolysis, promote the release of rapeseed oil again, through centrifugation, obtain high unsaturated active vegetable seed edible vegetable oil.Technical scheme of the present invention and production technique are as follows:

A, vegetable seed arrangement, decortication, the boiling water enzyme 3-20min that goes out;

Vegetable seed defibrination after B, the enzyme that will go out (colloidal mill, JMS-50 Langfang City Guan Tong Machinery Co., Ltd.) 3-20min;

C, composite polygalacturonase NCB-PE40, Pectinex Ultra SP-L, Viscozyme L, enzymolysis broken wall vegetable seed slurry;

D, employing Sumizyme MP Alcalase2.4L, take oil yield as evaluation index, optimize hydrolysis process: enzyme concentration 1-1.5%, hydrolysis temperature 60-65 ℃, enzymolysis time 1-2h, enzymolysis pH9-11;

E, centrifugation, by the screenings after enzymolysis under 2000-5000r/min condition, centrifugal 10-20min.

Four, embodiment

Below in conjunction with specific embodiment, the invention will be further described:

Embodiment 1

Get desquamated rapeseed 100g, boiling water boiling (solid-liquid ratio 1: 3) 5-10min, is cooled to room temperature by well-done vegetable seed, with colloidal mill (JMS-50 Langfang City Guan Tong Machinery Co., Ltd.), solid-liquid ratio 1: 4, defibrination 3-5min.The slurry of milled is added in enzymatic vessel, adjust pH to 4.5, controlled enzymatic hydrolysis temperature 50 C, enzyme addition 1%, polygalacturonase NCB-PE40, Pectinex Ultra SP-L, Viscozyme L proportioning are 1: 1: 1, enzymolysis 3h, regulate pH to 9, be warming up to 60 ℃, keep 1h, add 1% Sumizyme MP Alcalase2.4L, controlling pH is 9, enzymolysis 2h, the centrifugal 15min of 3500r/min, obtains edible vegetable oil.

Embodiment 2

Get desquamated rapeseed 100g, boiling water boiling (solid-liquid ratio 1: 3) 5-10min, is cooled to room temperature by well-done vegetable seed, with colloidal mill (JMS-50 Langfang City Guan Tong Machinery Co., Ltd.), solid-liquid ratio 1: 4, defibrination 3-5min.The slurry of milled is added in enzymatic vessel, adjust pH to 5,55 ℃ of controlled enzymatic hydrolysis temperature, enzyme addition 2%, polygalacturonase NCB-PE40, Pectinex Ultra SP-L, Viscozyme L proportioning are 1: 1: 2, enzymolysis 3h, regulate pH to 10, be warming up to 60 ℃, keep 1h, add 2% Sumizyme MP Alcalase2.4L, controlling pH is 10, enzymolysis 2h, the centrifugal 15min of 3500r/min, obtains edible vegetable oil.

Claims

1. biological enzyme is prepared a method for high unsaturated active rapeseed oil, it is characterized in that adopting following steps:

A, vegetable seed decortication: Semen Brassicae campestris cleaning, removal of impurities, broken, decortication;

B, the enzyme that goes out, defibrination: desquamated rapeseed is through boiling water go out enzyme 3-20min and then defibrination 3-20min;

C, enzymatic shell-broken: by adding compound wall breaking enzyme enzymolysis vegetable seed cell walls, promote grease to discharge;

D, appropriate protease hydrolyzed: adopt Alcalase2.4L to carry out appropriate enzymolysis, promote rapeseed oil to separate;

E, vegetable seed edible vegetable oil separate: by centrifugal the vegetable seed slurry after enzymolysis, obtain high unsaturated vegetable seed edible vegetable oil;

Wherein:

During described defibrination, solid-liquid ratio used is 1: 2-1: 10;

Described compound wall breaking enzyme and compound proportion thereof are: polygalacturonase NCB-PE40:Pectinex Ultra SP-L:Viscozyme L=0.5-1.5: 0.5-1.5: 0.2-2, prozyme addition is 0.1-10%, 50 ℃ of hydrolysis temperatures, enzymolysis time is 2-6h, enzymolysis pH4-5;

The addition of described protease A lcalase2.4L is 0.1-10%, hydrolysis temperature 60-65 ℃, enzymolysis time 1-4h, enzymolysis pH9-11; Vegetable seed slurry after described enzymolysis, centrifugal under 2000-5000r/min, obtain rapeseed oil, its unsaturated fatty acid content reaches more than 85%.