CN103045345A

CN103045345A - Production method for preparing highly unsaturated active rapeseed oil by biological enzyme method

Info

Publication number: CN103045345A
Application number: CN2012102200215A
Authority: CN
Inventors: 李春阳; 李跃; 张红城; 王帆
Original assignee: Jiangsu Academy of Agricultural Sciences
Current assignee: Jiangsu Academy of Agricultural Sciences
Priority date: 2012-06-29
Filing date: 2012-06-29
Publication date: 2013-04-17
Anticipated expiration: 2032-06-29
Also published as: CN103045345B

Abstract

The invention develops a method for preparing highly unsaturated active rapeseed oil by a biological enzyme method and belongs to the technical field of food processing. The production method comprises the following processes: peeling rapeseeds, inactivating enzyme in boiling water, grinding, carrying out composite enzymatic hydrolysis and breaking wall (NCB-PE40, Peetinex Ultra SP-L, Viscozyme L), alkaline extraction, protease hydrolysis (2.4L of Alcalase) and centrifugally separating to obtain the highly unsaturated active rapeseed oil. The method for preparing the highly unsaturated active rapeseed oil by the biological enzyme method has the characteristics that firstly, through the biological enzyme method to break the wall, the release of the rapeseed oil is promoted, and the oil output is greater than 95%; secondly, through proper protease enzymatic hydrolysis and the centrifugal separating (at 2000-5000rpm), the rapeseed oil is obtained, and the clean oil rate is higher than 60%; and thirdly, in comparison with the conventional pressing and extracting methods, the method disclosed by the invention is simple and easy to implement, and green and environment-friendly; the product is free from any pollution and solvent residue; the produced rapeseed oil contains more than 85% of unsaturated fatty acid, and is high in nutrition value and strong in activity.

Description

The production method of the high unsaturated active rapeseed oil of a kind of biological enzyme preparation

One, technical field

The invention provides the production method of the high unsaturated active rapeseed oil of a kind of biological enzyme preparation, belong to food processing technology field.

Two, background technology

Rape is crop in cruciferae, and 4.13 hundred million mu of world's cultivated areas, 5,100 ten thousand tons of output occupy the oil crops second.1.16 hundred million mu of China's rapeseed cultivation areas, 1,100 ten thousand tons of output account for respectively 24% and 25% of the world, are listed as first of the world.Semen Brassicae campestris is the important oil crops of China, accounts for 50% of domestic oil crops, and the rapeseed oil consumption accounts for more than 35% of domestic edible vegetable oil, occupies critical role on the edible oil market at home.

Industry preparation rapeseed oil is mainly taked milling process and lixiviation process.Milling process squeezes out grease mainly by mechanical external force from oil plant, product does not contain solvent, but its grouts resid amount is high, and oil yield efficiency is lower, and power consumption is large; Lixiviation process is selected special organic solvent, oil and grease extracting, and its high productivity, oil yield height are easily realized scale production, but solvent for use is mostly inflammable and explosive, and production security is relatively poor, and the finished product rapeseed oil has certain dissolvent residual, it is more that the leaching crude oil contains non-oil substances, and color and luster is darker, and is second-rate.Aqueous enzymatic method (claiming again water extraction) is emerging in recent years oil extracting process, utilizes the enzymolysis broken wall, and by the effect of water, by centrifugal edible vegetable oil is separated, and the edible vegetable oil quality is high, security is good.

The present invention adopts biological enzyme to produce rapeseed oil, by compound wall breaking enzyme the release rate of oil in the vegetable seed is reached more than 95%, by proteolysis, centrifugal, obtains the edible vegetable oil more than 60% again, and its unsaturated fatty acid content reaches more than 85%.Present method can significantly reduce production costs, and improves production security, can not produce detrimentally affect to environment, meets the human consumer to the demand of nutritive health-care grease, is conducive to the development of China's grease process deeply industry.

Three, summary of the invention

The present invention has developed the method that a kind of biological enzyme prepares high unsaturated active rapeseed oil.Take Semen Brassicae campestris as raw material, through arrangement, removal of impurities, peel, go out enzyme, defibrination, obtain the vegetable seed slurry; By biological enzyme (polygalacturonase NCB-PE40, Pectinex Ultra SP-L, Viscozyme L) compound broken wall and protease A lcalase2.4L hydrolysis, promote the release of rapeseed oil again, through centrifugation, obtain high unsaturated active vegetable seed edible vegetable oil.Technical scheme of the present invention and production technique are as follows:

A, vegetable seed arrangement, decortication, the boiling water enzyme 3-20min that goes out;

Vegetable seed defibrination behind B, the enzyme that will go out (colloidal mill, the logical mechanical company limited of JMS-50 Langfang City hat) 3-20min;

C, composite polygalacturonase NCB-PE40, Pectinex Ultra SP-L, Viscozyme L, enzymolysis broken wall vegetable seed slurry;

D, employing Sumizyme MP Alcalase2.4L take oil yield as evaluation index, optimize hydrolysis process: enzyme concentration 1-1.5%, hydrolysis temperature 60-65 ℃, enzymolysis time 1-2h, enzymolysis pH9-11;

E, centrifugation, with the screenings behind the enzymolysis under the 2000-5000r/min condition, centrifugal 10-20min.

Four, embodiment

The invention will be further described below in conjunction with specific embodiment:

Embodiment 1

Get desquamated rapeseed 100g, boiling water boiling (solid-liquid ratio 1: 3) 5-10min is cooled to room temperature with well-done vegetable seed, with colloidal mill (the logical mechanical company limited of JMS-50 Langfang City hat), solid-liquid ratio 1: 4, defibrination 3-5min.The slurry of milled is added in the enzymatic vessel, adjust pH to 4.5, the controlled enzymatic hydrolysis temperature 50 C, enzyme addition 1%, polygalacturonase NCB-PE40, Pectinex Ultra SP-L, Viscozyme L proportioning are 1: 1: 1, enzymolysis 3h, regulate pH to 9, be warming up to 60 ℃, keep 1h, add 1% Sumizyme MP Alcalase2.4L, control pH is 9, enzymolysis 2h, the centrifugal 15min of 3500r/min obtains edible vegetable oil.

Embodiment 2

Get desquamated rapeseed 100g, boiling water boiling (solid-liquid ratio 1: 3) 5-10min is cooled to room temperature with well-done vegetable seed, with colloidal mill (the logical mechanical company limited of JMS-50 Langfang City hat), solid-liquid ratio 1: 4, defibrination 3-5min.The slurry of milled is added in the enzymatic vessel, adjust pH to 5,55 ℃ of controlled enzymatic hydrolysis temperature, enzyme addition 2%, polygalacturonase NCB-PE40, Pectinex Ultra SP-L, Viscozyme L proportioning are 1: 1: 2, enzymolysis 3h, regulate pH to 10, be warming up to 60 ℃, keep 1h, add 2% Sumizyme MP Alcalase2.4L, control pH is 10, enzymolysis 2h, the centrifugal 15min of 3500r/min obtains edible vegetable oil.

Claims

1. a biological enzyme prepares the method for high unsaturated active rapeseed oil, it is characterized in that taking following technique and method:

A, vegetable seed decortication: Semen Brassicae campestris cleaning, removal of impurities, broken, decortication;

B, the enzyme that goes out, defibrination: desquamated rapeseed is through the boiling water enzyme 3-20min that goes out, and then defibrination (colloidal mill JMS-50, the logical mechanical company limited of Langfang City hat) 3-20min;

C, enzymatic shell-broken: compound wall breaking enzyme (polygalacturonase NCB-PE40, Pectinex Ultra SP-L, Viscozyme L), enzymolysis vegetable seed cell walls promotes grease to discharge;

D, appropriate protease hydrolyzed: adopt Alcalase2.4L appropriateness enzymolysis, promote the vegetable seed oil separating;

E, vegetable seed edible vegetable oil separate: the slurry of the vegetable seed behind the enzymolysis is centrifugal, obtain high unsaturated vegetable seed edible vegetable oil.

2. as claimed in claim 1, it is characterized in that: after the vegetable seed decortication, adopt boiling water effective passivation vegetable seed oxydase and sulphur glycosides enzyme, passivation time 3-20min.

3. as claimed in claim 1, it is characterized in that: during the vegetable seed defibrination, solid-liquid ratio is 1: 2-1: 10, and defibrination time 3-20min.

4. as claimed in claim 1, it is characterized in that: vegetable seed wall breaking enzyme compound proportion is: polygalacturonase NCB-PE40: PectinexUltra SP-L: Viscozyme L=0.5-1.5: 0.5-1.5: 0.2-2, the prozyme addition is 0.1-10%, 50 ℃ of hydrolysis temperatures, enzymolysis time 2-6h, enzymolysis pH4-5.

5. as claimed in claim 1, it is characterized in that: the proteolytic enzyme of selecting is Alcalase2.4L, enzyme addition 0.1-10%, hydrolysis temperature 60-65 ℃, enzymolysis time 1-4h, enzymolysis pH9-11.

6. as claimed in claim 1, it is characterized in that: the vegetable seed slurry behind the enzymolysis, centrifugal under 2000-5000 rev/min of condition, obtain vegetable seed edible vegetable oil, its unsaturated fatty acid content reaches more than 85%.