CN103652308A - Industrial preparation method of high-protein grape seed dregs - Google Patents
Industrial preparation method of high-protein grape seed dregs Download PDFInfo
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- CN103652308A CN103652308A CN201310663634.0A CN201310663634A CN103652308A CN 103652308 A CN103652308 A CN 103652308A CN 201310663634 A CN201310663634 A CN 201310663634A CN 103652308 A CN103652308 A CN 103652308A
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Abstract
The invention discloses an industrial preparation method of high-protein grape seed dregs, which comprises the following steps: (1) breaking the grape seeds and separating shells and kernels to obtain grape seed kernels; (2) softening and tempering the grape seed kernels with water, rolling, drying and degreasing; (3) performing low-temperature desolventizing on the degreased grape seed kernels, and adding an aqueous solution containing cellulase, hemicellulase and pectinase for an enzymolysis reaction; (4) after the enzymolysis reaction, performing solid-liquid separation on the material; adding a sodium hydroxide solution into the solid for dissolution, and stirring for reaction; after the reaction, performing solid-liquid separation; adjusting the pH value of the separated liquid to 3.8-4.2 to separate out protein dissolved in water; performing solid-liquid separation again to obtain the concentrate of grape seed kernel protein; (5) drying the concentrate of grape seed kernel protein to obtain the high-protein grape seed dregs. The method disclosed by the invention is simple in process and low in production cost; the product has high protein content and rich nutrition, wherein the protein content is greater than 49.5wt%, the ash content is less than 4wt%, the fiber content is less than 5wt%, and the amino acid content is greater than 95wt%.
Description
Technical field
The present invention relates to a kind of deep working method of grape pip, especially a kind of high protein grape pip dregs of rice industrialization preparation method.
Background technology
China's vinegrowing region is wide, and output increases year by year, and wherein 80% grape is for wine brewing, 13% left and right for edible, 7% left and right for fruit juice and other grape goods, so the schlempe of wine industry is the main source of grape discarded object.And the Production of Wine producer of China is also lower to the comprehensive utilization ratio of grape pip, be all much directly to lose, cause the significant wastage of resource.In grape pip, contain abundant unrighted acid, multivitamin and mineral matter and rich in protein amino acid, because process technology is limited at present, protein resource in grape pip can not well be developed, protein content is below 15%, only, as the general assorted dregs of rice or field as fertilizer sources also, caused the significant wastage of resource.
Summary of the invention
The technical problem to be solved in the present invention is to provide the simple high protein grape pip of a kind of technique dregs of rice industrialization preparation method.
For solving the problems of the technologies described above, method step of the present invention is:
(1) grape pip obtains grape pip benevolence through pulverizing, separation of hull from kernel;
(2) after grape pip benevolence uses water softening modified, strip plate, oven dry, then carry out degreasing;
(3) the grape pip benevolence after degreasing, through low temperature desolventizing, adds the mixed aqueous solution of cellulase, hemicellulase and pectase to carry out enzyme digestion reaction;
(4) enzyme digestion reaction finishes rear material Separation of Solid and Liquid, and solids adds sodium hydroxide solution to dissolve, stirring reaction; Reaction finishes rear Separation of Solid and Liquid, and the protein that parting liquid regulates pH to 3.8～4.2 to make to be dissolved in water is separated out; Separation of Solid and Liquid again, obtains the concentrate of grape pip benevolence albumen;
(5) concentrate of grape pip benevolence albumen after drying, can obtain the high protein grape pip dregs of rice.
In step of the present invention (2), adopt non-polar solven to carry out degreasing 45～49 ℃ of extractions.
In step of the present invention (2), 55～60 ℃ of modified 45～55min of water softening for grape pip benevolence.
In step of the present invention (2), softening grape pip benevolence after modified keeps the moisture of 7～9wt% to carry out strip plate; Dry to moisture 3～4wt% and carry out degreasing.
In step of the present invention (2), the temperature of oven dry is 60～79 ℃.
In step of the present invention (3), cellulase, hemicellulase and pectase are respectively 80～180g/L, 60～120g/L and 60～120g/L in the concentration of mixed aqueous solution; The solid-liquid ratio of enzyme digestion reaction is 1:2～3.5.
In step of the present invention (3), enzyme digestion reaction temperature is 30～35 ℃, and the reaction time is 1080～1440min.
In step of the present invention (4), solids adds 7～11mmol/L sodium hydroxide solution to dissolve; Reaction temperature is 40～45 ℃, and the reaction time is 60～80min.
The beneficial effect that adopts technique scheme to produce is: the present invention obtains grape pip the grape pip dregs of rice of high protein through steps such as broken, separated, softening modified, pressed compact, oven dry, degreasing, low temperature desolventizing, enzyme digestion reaction and isoelectric points separated (protein is separated out).Owing to having adopted pretreatment of raw material and rational separated, extraction process, thereby obtain the high-quality grapes seed dregs of rice of high protein, low ash content, low fiber, high amino acid content, greatly promoted on the one hand the using value of the grape pip dregs of rice, also can alleviate to a certain extent domestic to feeding high protein demand simultaneously.Technique of the present invention is simple, and production cost is low, and the protein content obtaining is high, nutritious, and wherein protein content is greater than 49.5wt%, and ash content is less than 4wt%, and fiber is less than 5wt%, and amino acid content is greater than 95wt%.
Accompanying drawing explanation
Below in conjunction with the drawings and specific embodiments, the present invention is further detailed explanation.
Fig. 1 is flowage structure schematic diagram of the present invention.
The specific embodiment
Embodiment 1: the concrete steps of this high protein grape pip dregs of rice industrialization preparation method are as described below.
Shown in Fig. 1, grape pip will Renhe shell be pulverized, is obtained grape pip benevolence after separation through pulverizer, the modified 45min of water softening of 55 ℃ for grape pip benevolence, and the moisture of maintenance 7wt% enters crushing roll strip plate, then at 70 ℃, dry to moisture 3wt%, send into degreasing infuser; Adopt non-polar solven ethyl acetate spraying and extracting stage by stage, 45 ℃ of extraction temperature, time 130min, extraction obtains miscella; Grape pip benevolence after degreasing is through low temperature desolventizing, removal of solvents is fallen, according to solid-liquid ratio, be 1:2(g/ml) add the mixed aqueous solution of cellulase 80g/L, hemicellulase 65g/L and pectase 60g/L to carry out enzyme digestion reaction, reaction temperature is controlled at 30 ℃, and the reaction time is controlled at 1080min; Enzyme digestion reaction finishes rear material and filters, and lower floor's filter residue adds the sodium hydroxide solution of 7mmol/L to dissolve, and temperature is controlled at 40 ℃ and carries out stirring reaction, and the reaction time is controlled at 60min; Reaction finishes rear isolated by filtration, and filtrate enters into blend tank, by the protein that regulates pH to 3.8 to make to be dissolved in water, separates out, and by horizontal centrifuge, carries out separation, obtains the concentrate of grape pip benevolence albumen; Concentrate obtains through low temperature drying the grape pip dregs of rice that protein content is 49.8wt%, ash content 2.8wt%, fiber 4.6wt%, amino acid content 95.8wt%.
Embodiment 2: the concrete steps of this high protein grape pip dregs of rice industrialization preparation method are as described below.
Grape pip obtain grape pip benevolence after pulverizer will Renhe shell be pulverized separation, the modified 50min of water softening of 58 ℃ for grape pip benevolence, and the moisture of maintenance 9wt% enters crushing roll strip plate, then at 79 ℃, dries to moisture 3.5wt%, sends into degreasing infuser; Adopt non-polar solven benzinum spraying and extracting stage by stage, 48 ℃ of extraction temperature, time 130min, obtains miscella; Grape pip benevolence after degreasing is through low temperature desolventizing, removal of solvents is fallen, according to solid-liquid ratio, be 1:3(g/ml) add the mixed aqueous solution of cellulase 90g/L, hemicellulase 60g/L and pectase 90g/L to carry out enzyme digestion reaction, reaction temperature is controlled at 35 ℃, and the reaction time is controlled at 1400min; Enzyme digestion reaction finishes rear material and filters, and lower floor's filter residue adds the sodium hydroxide solution of 8.5mmol/L to dissolve, and temperature is controlled at 45 ℃ and carries out stirring reaction, and the reaction time is controlled at 80min; Reaction finishes rear isolated by filtration, and filtrate enters into blend tank, by the protein that regulates pH to 3.9 to make to be dissolved in water, separates out, and by horizontal centrifuge, carries out separation, obtains the concentrate of grape pip benevolence albumen; Concentrate obtains through low temperature drying the grape pip dregs of rice that protein content is 50.6wt%, ash content 3.7wt%, fiber 4.2wt%, amino acid 97.6wt%.
Embodiment 3: the concrete steps of this high protein grape pip dregs of rice industrialization preparation method are as described below.
Grape pip obtain grape pip benevolence after pulverizer will Renhe shell be pulverized separation, the modified 45min of water softening of 57 ℃ for grape pip benevolence, and the moisture of maintenance 8wt% enters crushing roll strip plate, then dries to moisture 4wt% for 60 ℃, sends into degreasing infuser; Adopt non-polar solven carrene spraying and extracting stage by stage, 48 ℃ of extraction temperature, the time, 160min obtained miscella; Grape pip benevolence after degreasing is through low temperature desolventizing, removal of solvents is fallen, according to solid-liquid ratio, be that 1:3 adds the mixed aqueous solution of cellulase 100g/L, hemicellulase 90g/L and pectase 80g/L to carry out enzyme digestion reaction, reaction temperature is controlled at 32 ℃, and the reaction time is controlled at 1250min; Enzyme digestion reaction finishes rear material and filters, and lower floor's filter residue adds the sodium hydroxide solution of 10mmol/L to dissolve, and temperature is controlled at 42 ℃ and carries out stirring reaction, and the reaction time is controlled at 75min; Reaction finishes rear isolated by filtration, and filtrate enters into blend tank, by the protein that regulates pH to 4.1 to make to be dissolved in water, separates out, and by horizontal centrifuge, carries out separation, obtains the concentrate of grape pip benevolence albumen; Concentrate obtains through low temperature drying the grape pip dregs of rice that protein content is 51wt%, ash content 3.7wt%, fiber 3.5wt%, amino acid content 96.1wt%.
Embodiment 4: the concrete steps of this high protein grape pip dregs of rice industrialization preparation method are as described below.
Grape pip obtain grape pip benevolence after pulverizer will Renhe shell be pulverized separation, the modified 55min of water softening of 56 ℃ for grape pip benevolence, and the moisture of maintenance 7.5wt% enters crushing roll strip plate, then dries to moisture 3wt%, sends into degreasing infuser; Adopt non-polar solven cyclohexane spraying and extracting stage by stage, 46 ℃ of extraction temperature, the time, 140min obtained miscella; Grape pip benevolence after degreasing is through low temperature desolventizing, removal of solvents is fallen, according to solid-liquid ratio, be 1:2.5(g/ml) add the mixed aqueous solution of cellulase 140g/L, hemicellulase 100g/L and pectase 120g/L to carry out enzyme digestion reaction, reaction temperature is controlled at 34 ℃, and the reaction time is controlled at 1440min; Enzyme digestion reaction finishes rear material and filters, and lower floor's filter residue adds the sodium hydroxide solution of 11mmol/L to dissolve, and temperature is controlled at 45 ℃ and carries out stirring reaction, and the reaction time is controlled at 70min; Reaction finishes rear isolated by filtration, and filtrate enters into blend tank, by the protein that regulates pH to 4.0 to make to be dissolved in water, separates out, and by horizontal centrifuge, carries out separation, obtains the concentrate of grape pip benevolence albumen; Concentrate obtains through low temperature drying the grape pip dregs of rice that protein content is 50.4wt%, ash content 3.6wt%, fiber 4.6wt%, amino acid 96.4wt%.
Embodiment 5: the concrete steps of this high protein grape pip dregs of rice industrialization preparation method are as described below.
Grape pip obtains grape pip benevolence after Renhe shell being pulverized to separation through pulverizer, and grape pip benevolence enters crushing roll strip plate with the modified moisture to keeping 8.5wt% of water softening of 60 ℃, then dries to moisture 4wt%, sends into degreasing infuser; Adopt non-polar solven ether to spray stage by stage, 49 ℃ of extraction temperature, obtain miscella; Grape pip benevolence after degreasing is through low temperature desolventizing, removal of solvents is fallen, according to solid-liquid ratio, be that 1:3.5 adds the mixed aqueous solution of cellulase 180g/L, hemicellulase 120g/L and pectase 105g/L to carry out enzyme digestion reaction, reaction temperature is controlled at 35 ℃, and the reaction time is controlled at 1280min; Enzyme digestion reaction finishes rear material and filters, lower floor's filter residue adds the sodium hydroxide solution of 9.5mmol/L to dissolve, temperature is controlled at 45 ℃ and carries out stirring reaction, reaction time is controlled at 65min, reaction finishes rear isolated by filtration, and filtrate enters into blend tank, by the protein that regulates pH to 4.2 to make to be dissolved in water, separates out, by horizontal centrifuge, carry out separation, obtain the concentrate of grape pip benevolence albumen; Concentrate obtains through low temperature drying the grape pip dregs of rice that protein content is 49.8wt%, ash content 3.2wt%, fiber 3.9wt%, amino acid 95.4wt%.
Claims (8)
1. a high protein grape pip dregs of rice industrialization preparation method, is characterized in that, the method step is:
(1) grape pip obtains grape pip benevolence through pulverizing, separation of hull from kernel;
(2) after grape pip benevolence uses water softening modified, strip plate, oven dry, then carry out degreasing;
(3) the grape pip benevolence after degreasing, through low temperature desolventizing, adds the mixed aqueous solution of cellulase, hemicellulase and pectase to carry out enzyme digestion reaction;
(4) enzyme digestion reaction finishes rear material Separation of Solid and Liquid, and solids adds sodium hydroxide solution to dissolve, stirring reaction; Reaction finishes rear Separation of Solid and Liquid, and the protein that parting liquid regulates pH to 3.8～4.2 to make to be dissolved in water is separated out; Separation of Solid and Liquid again, obtains the concentrate of grape pip benevolence albumen;
(5) concentrate of grape pip benevolence albumen after drying, can obtain the high protein grape pip dregs of rice.
2. high protein grape pip dregs of rice industrialization preparation method according to claim 1, is characterized in that: in described step (2), adopt non-polar solven to carry out degreasing 45～49 ℃ of extractions.
3. high protein grape pip dregs of rice industrialization preparation method according to claim 1, is characterized in that: in described step (2), and 55～60 ℃ of modified 45～55min of water softening for grape pip benevolence.
4. high protein grape pip dregs of rice industrialization preparation method according to claim 1, is characterized in that: in described step (2), the grape pip benevolence after softening modified keeps the moisture of 7～9wt% to carry out strip plate; Dry to moisture 3～4wt% and carry out degreasing.
5. according to the high protein grape pip dregs of rice industrialization preparation method described in claim 1-4 any one, it is characterized in that: in described step (2), the temperature of oven dry is 60～79 ℃.
6. high protein grape pip dregs of rice industrialization preparation method according to claim 1, it is characterized in that: in described step (3), cellulase, hemicellulase and pectase are respectively 80～180g/L, 60～120g/L and 60～120g/L in the concentration of mixed aqueous solution; The mass volume ratio of enzyme digestion reaction feed liquid is 1:2～3.5.
7. according to the high protein grape pip dregs of rice industrialization preparation method described in claim 1 or 6, it is characterized in that: in described step (3), enzyme digestion reaction temperature is 30～35 ℃, and the reaction time is 1080～1440min.
8. high protein grape pip dregs of rice industrialization preparation method according to claim 1, is characterized in that: in described step (4), solids adds 7～11mmol/L sodium hydroxide solution to dissolve; Reaction temperature is 40～45 ℃, and the reaction time is 60～80min.
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Cited By (3)
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| CN104187549A (en) * | 2014-09-22 | 2014-12-10 | 王婧婧 | Method preparing tea flavored spice through grade seeds |
| CN109369774A (en) * | 2018-11-23 | 2019-02-22 | 盐城工学院 | The extracting method of protein in a kind of acer truncatum seed dregs of rice |
| CN113755238A (en) * | 2020-06-04 | 2021-12-07 | 陕西为康生物科技股份有限公司 | Processing method of high-protein pepper seed meal |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104187549A (en) * | 2014-09-22 | 2014-12-10 | 王婧婧 | Method preparing tea flavored spice through grade seeds |
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| CN109369774A (en) * | 2018-11-23 | 2019-02-22 | 盐城工学院 | The extracting method of protein in a kind of acer truncatum seed dregs of rice |
| CN113755238A (en) * | 2020-06-04 | 2021-12-07 | 陕西为康生物科技股份有限公司 | Processing method of high-protein pepper seed meal |
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Application publication date: 20140326 |