CN110584130A - Preparation method of fructus cannabis protein powder containing water-soluble cannabidiol - Google Patents
Preparation method of fructus cannabis protein powder containing water-soluble cannabidiol Download PDFInfo
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- CN110584130A CN110584130A CN201910969899.0A CN201910969899A CN110584130A CN 110584130 A CN110584130 A CN 110584130A CN 201910969899 A CN201910969899 A CN 201910969899A CN 110584130 A CN110584130 A CN 110584130A
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/006—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from vegetable materials
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/14—Vegetable proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
- A23L29/35—Degradation products of starch, e.g. hydrolysates, dextrins; Enzymatically modified starches
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/185—Vegetable proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Polymers & Plastics (AREA)
- Nutrition Science (AREA)
- Biochemistry (AREA)
- Mycology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Botany (AREA)
- Medicines Containing Plant Substances (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
The invention discloses a preparation method of fructus cannabis protein powder containing water-soluble cannabidiol, which is prepared by adding a certain proportion of water-soluble Cannabidiol (CBD) into the fructus cannabis protein powder, and has good health-care effects of improving sleep, resisting aging, relaxing bowel, improving immunity of organisms and the like. The invention improves the water solubility of CBD through special process on the premise of not changing the molecular structure; the protein and carbohydrate are the most abundant substances in the fructus cannabis except the fructus cannabis oil, and are an excellent dietary protein source. The fructus cannabis protein powder is prepared by mixing fructus cannabis with waterSupercritical CO after mechanical pressing of hemp seed2The method has the advantages that the grease in the fructus cannabis is removed by the two-step extraction method, then the degreased cake meal is subjected to superfine grinding to obtain fructus cannabis protein powder, the nutrition is rich, no loss exists, the supercritical CO2 extraction efficiency is high, the grease is completely removed, no solvent residue exists, the method is environment-friendly, healthy and safe, and the high-quality fructus cannabis protein powder can be obtained.
Description
Technical Field
The invention belongs to the technical field of hemp seed processing, and particularly relates to a preparation method of hemp protein powder containing water-soluble cannabidiol.
Background
The hemp, also known as China hemp and castor, is one of the traditional medicinal materials and health food raw materials in China. The national ministry of health 2002 documents list fructus cannabis in a list of articles which are both medicines and foods, so that the development and utilization of fructus cannabis foods are regulated and guaranteed.
The dehulled hemp seed contains 46.5% oil, 34.6% protein, and 11.6% carbohydrate, the balance being vitamins and minerals. Hemp seed protein contains 18 known amino acids, including 10 human essential amino acids (EEAs) in a well-balanced ratio. A prominent aspect of the hemp seed protein is the high content of arginine (123mg/g protein) and histidine (27 mg/g protein), both of which are important for childhood growth, and the sulfur-containing amino acid cystine (16 mg/g protein) required for the synthesis of the necessary enzymes. In addition, the hemp seed protein does not contain tryptophan inhibitory factor, does not affect the absorption of protein, has a total protein content equivalent to that of soybeans, which is higher than that of nuts, other seeds, milk products, meat, fish or poultry, but does not contain some of the oligosaccharides and sensitization factors of soybeans, and does not cause gastric distention, regurgitation and allergic reactions. 65% of the cannabis protein is edestin (edestin), which is present only in the cannabis seeds. Edestin has good effect on promoting digestion, is relatively phosphorus-free, and is a cell DNA skeleton. In addition, 35% of the protein is albumin, and the protein is also high-quality protein, so that the fructus cannabis is a very excellent protein source. The fructus Cannabis protein powder has effects of loosening bowel to relieve constipation, repairing myocardial damage, etc., and can be used as natural food health product additive.
Cannabidiol (CBD) is a non-addictive component in cannabis sativa, is not separated from other animals and plants at present, has high medicinal value, and has the functions of resisting epilepsy, resisting psychosis, resisting depression, relieving pain, relieving nausea caused by cancer chemotherapy, treating asthma and the like. In recent years, scientists also find that Cannabidiol (CBD) is a powerful antioxidant, has the function of blocking the adverse effect of certain drugs on human nerves, and has a series of physiological activity functions of blocking breast cancer metastasis, resisting rheumatoid arthritis, resisting insomnia and the like. However, cannabidiol is a fat-soluble compound and insoluble in water, so that the application field of CBD is limited, and the bioavailability of CBD is reduced by direct use.
The patent CN 105994930A discloses a processing technology of high-purity fructus cannabis protein powder, the fructus cannabis is used as a raw material, grease is removed through mechanical squeezing and subcritical extraction, then sodium chloride saturated solution and cellulase are utilized to degrade, separate and purify protein, an extractant of subcritical extraction grease is butane, solvent residue is easily caused, a large amount of organic solvent is consumed, and the environment is not friendly; patent CN 108497482A discloses a method for preparing industrial hemp seed kernel whole powder, which mainly removes oil by low-temperature cold pressing and then micronizes to prepare hemp seed powder, the invention only adopts low-temperature cold pressing to remove oil, grease is difficult to remove completely, and certain influence is brought to the quality of hemp seed protein powder; the patent CN 105061554B discloses a preparation method of hemp concentrated protein powder rich in cannabidiol, which takes denatured hemp meal as a raw material, and prepares the hemp concentrated protein powder containing CBD by alkali solution primary extraction, colloid mill grinding, jet cooker heat treatment, centrifugation, ultrafiltration and spray drying, on one hand, the cannabidiol is mainly concentrated in flowers and leaves of hemp or industrial hemp, and the cannabidiol content in hemp is extremely low; on the other hand, the CBD contained in the protein powder obtained by the method is not treated and is insoluble in water, so that the application field of the protein powder and the bioavailability of the CBD are limited, and the effect of the protein powder cannot be fully exerted.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a preparation method of fructus cannabis protein powder containing water-soluble cannabidiol, which combines the physiological effects of cannabidiol and fructus cannabis protein powder, prepares the water-soluble cannabidiol by a cyclodextrin inclusion method, improves the water solubility and bioavailability of the water-soluble cannabidiol, and can exert a plurality of physiological effects by adding the obtained water-soluble cannabidiol into the fructus cannabis protein powder in a synergistic manner. The product of the invention has rich nutrition and no loss, and has good health care effects of improving sleep, resisting aging, relaxing bowel, improving immunity of organisms and the like. The method adopts a two-step method of mechanical squeezing and supercritical CO2 extraction to remove the grease, has high efficiency, thorough grease removal, no solvent residue, environmental friendliness, health and safety, and can obtain the high-quality fructus cannabis protein powder.
The purpose of the invention is realized by the following technical scheme:
1. a fructus Cannabis protein powder containing water soluble cannabidiol is prepared from (by weight parts) fructus Cannabis protein powder 85 ~ 95, and water soluble cannabidiol 5 ~ 15.
2. The preparation method of the water-soluble cannabidiol comprises the following steps: firstly, dissolving high-purity CBD crystals by using 2-5 times of ethanol to form a solution; heating and dissolving alpha-cyclodextrin by using 1-5 times of pure water or ethanol-water, adding the solution I after the alpha-cyclodextrin is completely dissolved, and stirring and mixing uniformly; clathrating, namely uniformly mixing the solution formed by the first step and the second step, carrying out cannabidiol clathration by using a high-pressure homogenization method, and after the clathration is finished, carrying out reduced pressure concentration by using a rotary evaporator to remove most of solvent and water; drying, and vacuum drying the material to obtain the cannabidiol clathrate (water-soluble).
3. A preparation method of fructus cannabis protein powder containing water-soluble cannabidiol comprises the following steps:
step 1, shelling: selecting the hemp seeds which are not mildewed, not damaged by worms and full in seeds as raw materials, and putting the raw materials into a huller to carry out hulling treatment.
Step 2, removing impurities: and (4) putting the shelled fructus cannabis into a proportion selector to remove impurities, and ensuring that the fructus cannabis does not contain other impurities.
And step 3, cleaning and drying: repeatedly cleaning the fructus cannabis particles, removing surface dust, and putting into drying equipment for drying treatment to ensure that the water content is reduced to below 10%.
And 4, mechanically squeezing the fructus cannabis at normal temperature, and separating oil to obtain primary cake.
And 5, performing supercritical CO2 extraction after mechanical pressing, and removing redundant grease to obtain protein meal.
And 6, carrying out ultramicro physical crushing on the protein meal, and sieving by using a 200-mesh sieve to obtain the fructus cannabis protein powder.
And 7, uniformly mixing 5 ~ 15 parts of water-soluble cannabidiol and 85 ~ 95 parts of fructus cannabis protein powder, and sieving the mixture with a 200-mesh sieve to obtain the protein powder.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the invention, under the premise of not changing the molecular structure, the water-insoluble cannabidiol is embedded with the CBD with higher purity by a cyclodextrin inclusion method, so that the water solubility of the CBD is changed, and the water solubility and bioavailability of the CBD are improved.
2. The cannabidiol and the fructus cannabis protein are pure plant extract components, and the cannabidiol and the fructus cannabis protein can be used in cooperation to exert a plurality of physiological effects, so that the product has good health-care effects of improving sleep, resisting aging, resisting oxidation, relaxing bowel, improving immunity of organisms and the like, and can be used as a functional food or a health-care product additive for human or pets.
3. The method adopts a two-step method of mechanical squeezing and supercritical CO2 extraction to remove the hemp seed oil, has high efficiency, thorough oil removal, no solvent residue, environmental protection, health and safety, and can obtain high-quality hemp seed protein powder.
Detailed Description
The following examples are merely illustrative of the present invention and do not limit the scope of the present invention in any way. For process parameters or conditions not specifically mentioned, it can be carried out with reference to conventional techniques. It will be apparent to those skilled in the art that equivalent embodiments or modifications without departing from the technical spirit of the present invention are within the scope of the present invention.
Example 1A method for preparing fructus Cannabis protein powder containing water soluble cannabidiol
(1) The preparation of water-soluble cannabidiol comprises the following steps:
accurately weighing 100g of cannabidiol solid powder (with the purity of more than 99%) and 500g of ethanol, and adding the 100g of cannabidiol solid powder and the 500g of ethanol into a 1L round-bottom flask with a single mouth; mechanically stirring for 30min to dissolve cannabidiol solid completely; cooling to room temperature for later use after complete dissolution.
Accurately weighing 300g of alpha-cyclodextrin and 900g of first-grade purified water, adding the alpha-cyclodextrin and the first-grade purified water into a 2L single-mouth round-bottom flask, placing the flask into a water bath kettle at 40 ℃, and mechanically stirring for 30min to fully dissolve the alpha-cyclodextrin in the water to form a transparent solution; and (4) after the solution is completely dissolved, adding the solution in the step (i) into the solution at one time, and continuing stirring for 10 min.
Clathrating, namely putting the mixed solution prepared in the step II into a high-pressure homogenizer, starting a high-pressure pump, and homogenizing for 3 times at the pressure of 35MPa and the temperature of 25 ℃; after the high-pressure homogenization treatment is finished, carrying out reduced pressure concentration by using a rotary evaporator to remove ethanol and water; the vacuum degree is-0.1 MPa, and the temperature is 70 ℃.
Drying, crushing the crude product and solid blocks formed after vacuum concentration into powder, spreading the powder in a watch glass, placing the watch glass in a vacuum drying oven, and performing vacuum drying for 72 hours at the vacuum degree of-0.08 MPa and the temperature of 35 ℃. Thus obtaining the cannabidiol clathrate (water-soluble).
(2) A preparation method of fructus cannabis protein powder containing water-soluble cannabidiol comprises the following steps:
step 1, shelling: selecting the hemp seeds which are not mildewed, not damaged by worms and full in seeds as raw materials, and putting the raw materials into a huller to carry out hulling treatment.
Step 2, removing impurities: and (4) putting the shelled fructus cannabis into a proportion selector to remove impurities, and ensuring that the fructus cannabis does not contain other impurities.
And step 3, cleaning and drying: repeatedly cleaning the fructus cannabis particles, removing surface dust, and putting into drying equipment for drying treatment to ensure that the water content is reduced to below 10%.
And 4, mechanically squeezing the fructus cannabis at normal temperature, and separating oil to obtain primary cake.
Step 5, performing supercritical CO2 extraction after mechanical squeezing, and removing redundant grease to obtain protein meal; the extraction and separation conditions are as follows: the extraction temperature is 40 deg.C, the pressure is 25MPa, and the separation temperature is 45 deg.C, and the pressure is 5 MPa.
And 6, carrying out ultramicro physical crushing on the protein meal, and sieving by using a 200-mesh sieve to obtain the fructus cannabis protein powder.
And 7, uniformly mixing 5 parts of water-soluble cannabidiol and 95 parts of fructus cannabis protein powder, and sieving by a 200-mesh sieve to obtain the fructus cannabis protein powder containing 5% of water-soluble CBD.
Example 2A method for preparing fructus Cannabis protein powder containing water soluble cannabidiol
(1) The preparation of water-soluble cannabidiol was performed as in example 1.
(2) A preparation method of fructus cannabis protein powder containing water-soluble cannabidiol comprises the following steps:
step 1, shelling: selecting the hemp seeds which are not mildewed, not damaged by worms and full in seeds as raw materials, and putting the raw materials into a huller to carry out hulling treatment.
Step 2, removing impurities: and (4) putting the shelled fructus cannabis into a proportion selector to remove impurities, and ensuring that the fructus cannabis does not contain other impurities.
And step 3, cleaning and drying: repeatedly cleaning the fructus cannabis particles, removing surface dust, and putting into drying equipment for drying treatment to ensure that the water content is reduced to below 10%.
And 4, mechanically squeezing the fructus cannabis at normal temperature, and separating oil to obtain primary cake.
Step 5, performing supercritical CO2 extraction after mechanical squeezing, and removing redundant grease to obtain protein meal; the extraction and separation conditions are as follows: the extraction temperature is 45 ℃, the pressure is 20MPa, and the separation temperature is 50 ℃, and the pressure is 6 MPa.
And 6, carrying out ultramicro physical crushing on the protein meal, and sieving by using a 200-mesh sieve to obtain the fructus cannabis protein powder.
And 7, uniformly mixing 10 parts of water-soluble cannabidiol and 90 parts of fructus cannabis protein powder, and sieving by a 200-mesh sieve to obtain the fructus cannabis protein powder containing 10% of water-soluble CBD.
Example 3A method for preparing fructus Cannabis protein powder containing water soluble cannabidiol
(1) The preparation of water-soluble cannabidiol was performed as in example 1.
(2) A preparation method of fructus cannabis protein powder containing water-soluble cannabidiol comprises the following steps:
step 1, shelling: selecting the hemp seeds which are not mildewed, not damaged by worms and full in seeds as raw materials, and putting the raw materials into a huller to carry out hulling treatment.
Step 2, removing impurities: and (4) putting the shelled fructus cannabis into a proportion selector to remove impurities, and ensuring that the fructus cannabis does not contain other impurities.
And step 3, cleaning and drying: repeatedly cleaning the fructus cannabis particles, removing surface dust, and putting into drying equipment for drying treatment to ensure that the water content is reduced to below 10%.
And 4, mechanically squeezing the fructus cannabis at normal temperature, and separating oil to obtain primary cake.
Step 5, performing supercritical CO2 extraction after mechanical squeezing, and removing redundant grease to obtain protein meal; the extraction and separation conditions are as follows: the extraction temperature is 50 ℃, the pressure is 28MPa, the separation temperature is 40 ℃, and the pressure is 7 MPa.
And 6, carrying out ultramicro physical crushing on the protein meal, and sieving by using a 200-mesh sieve to obtain the fructus cannabis protein powder.
And 7, uniformly mixing 15 parts of water-soluble cannabidiol and 80 parts of fructus cannabis protein powder, and sieving by a 200-mesh sieve to obtain the fructus cannabis protein powder containing 15% of water-soluble CBD.
Claims (10)
1. A preparation method of fructus cannabis protein powder containing water-soluble cannabidiol comprises 85 ~ 95 parts of fructus cannabis protein powder and 5 ~ 15 parts of water-soluble cannabidiol by weight.
2. The method for preparing fructus cannabis protein powder containing water-soluble cannabidiol as claimed in claim 1, the method for preparing water-soluble cannabidiol comprises the following steps: firstly, dissolving high-purity CBD crystals by using 2-5 times of ethanol to form a solution; heating and dissolving alpha-cyclodextrin by using 1-5 times of pure water or ethanol-water, adding the solution I after the alpha-cyclodextrin is completely dissolved, and stirring and mixing uniformly; clathrating, namely uniformly mixing the solution formed by the first step and the second step, carrying out cannabidiol clathration by using a high-pressure homogenization method, and after the clathration is finished, carrying out reduced pressure concentration by using a rotary evaporator to remove most of solvent and water; drying, and vacuum drying the material to obtain the cannabidiol clathrate (water-soluble).
3. The method for preparing hemp seed protein powder containing water-soluble cannabidiol according to claims 1 and 2, which comprises the following steps:
step 1, shelling: selecting mildew-free, worm-eaten-free and full-grained hemp seeds as raw materials, and putting the raw materials into a huller to carry out hulling treatment;
step 2, removing impurities: putting the shelled fructus cannabis into a proportion selector for impurity removal to ensure that the fructus cannabis does not contain other impurities;
and step 3, cleaning and drying: repeatedly cleaning the fructus cannabis particles, removing surface dust, and putting into drying equipment for drying treatment to ensure that the water content is reduced to below 10%;
step 4, mechanically squeezing the fructus cannabis at normal temperature, and separating oil to obtain primary cake;
step 5, performing supercritical CO2 extraction after mechanical squeezing, and removing redundant grease to obtain protein meal;
step 6, carrying out ultramicro physical crushing on the protein meal, and sieving by a 200-mesh sieve to obtain fructus cannabis protein powder;
and 7, uniformly mixing the water-soluble cannabidiol and the fructus cannabis protein powder according to the proportion, and sieving by a 200-mesh sieve to obtain the protein powder.
4. The method for preparing fructus cannabis protein powder containing water soluble cannabidiol as claimed in claim 3, wherein the fructus cannabis protein powder in step 6 has a protein content of 55 ~ 60%.
5. The preparation method of fructus cannabis protein powder containing water soluble cannabidiol as claimed in claim 3, wherein the supercritical CO2 extraction conditions of the fructus cannabis oil are extraction temperature of 30 deg.C ~ 50 deg.C, preferably 40 deg.C, pressure of 12 ~ 30MPa, preferably 25MPa, separation temperature of 40 ~ 55 deg.C, preferably 45 deg.C, pressure of 4 ~ 8MPa, preferably 6 MPa.
6. The method of claim 2, wherein the cannabidiol has a purity of 99% or more; the content of cannabidiol in the water-soluble cannabidiol is more than 90%.
7. The method of preparing cannabidiol as claimed in claim 2, wherein the amount of pure water used in step (2) is 1-5 times the mass of α -cyclodextrin, and the dissolution temperature is 30-60 ℃.
8. The method of claim 2, wherein the step (3) of homogenizing under high pressure at 25 ℃ under 30-50MPa for 2-3 times.
9. The method of claim 2, wherein the step (4) of vacuum drying is performed in a vacuum drying oven at a temperature of 30-60 ℃ under a vacuum degree of-0.08 MPa for 60-120 h.
10. The application of the preparation method of the fructus cannabis protein powder containing water-soluble cannabidiol is characterized in that the fructus cannabis protein powder can be used as a functional food or a health-care product additive for human or pets.
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| CN112167619A (en) * | 2020-06-29 | 2021-01-05 | 武汉林宝莱生物科技有限公司 | Protein powder formula of cannabidiol and preparation process thereof |
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| KR20220141114A (en) * | 2021-04-12 | 2022-10-19 | 농업회사법인 제이헴프코리아 주식회사 | Method of producing a powder of Cannabis seeds oil cake |
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| CN112167619A (en) * | 2020-06-29 | 2021-01-05 | 武汉林宝莱生物科技有限公司 | Protein powder formula of cannabidiol and preparation process thereof |
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| KR102593702B1 (en) * | 2021-04-12 | 2023-10-25 | 농업회사법인 제이헴프코리아 주식회사 | Method of producing a powder of Cannabis seeds oil cake |
| CN115707478A (en) * | 2021-08-18 | 2023-02-21 | 昆药集团股份有限公司 | Composition with anti-aging effect and preparation method and application thereof |
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