CN114097968A - Preparation method of plant protein polypeptide beverage for improving sleep - Google Patents
Preparation method of plant protein polypeptide beverage for improving sleep Download PDFInfo
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- CN114097968A CN114097968A CN202111334275.5A CN202111334275A CN114097968A CN 114097968 A CN114097968 A CN 114097968A CN 202111334275 A CN202111334275 A CN 202111334275A CN 114097968 A CN114097968 A CN 114097968A
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- protein polypeptide
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Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/06—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Non-Alcoholic Beverages (AREA)
Abstract
The invention provides a preparation method of a vegetable protein polypeptide beverage for improving sleep, which comprises the steps of carrying out hulling, grinding and degreasing on hemp seeds to obtain pure hemp seed protein powder; the defatted protein powder is subjected to grinding, enzymolysis by compound protease, enzyme deactivation, deodorization and filtration to obtain hemp seed protein polypeptide liquid, and then natural flavoring agents and natural stabilizing agents are added according to the weight ratio, and the hemp seed protein polypeptide liquid is prepared by high-pressure homogenization, canning and sterilization. The protein polypeptide liquid is prepared from the hemp seeds directly or from hemp seed meal after oil extraction, so that hemp seed resources are comprehensively utilized, defatted hemp seed protein is used for preparing the protein polypeptide liquid, the influence of oil and fat on thermal instability along with the protein liquid is avoided, the stability of the protein polypeptide liquid is ensured, the pulping temperature is strictly controlled, low-temperature pulping is adopted, the protein activity is fully ensured, the original nutritional value of the hemp seed protein is fully exerted, natural flavoring agents are adopted, the harm of the flavoring agents such as sucralose and the like to intestinal flora is avoided, the addition is more natural and pure, and the protein polypeptide liquid is suitable for various crowds.
Description
Technical Field
The invention relates to the technical field of food processing, in particular to a preparation method of a vegetable protein polypeptide beverage for improving sleep.
Background
With the release of the planting and processing of industrial hemp, the planting of industrial hemp is increased year by year from 2015, and a large amount of industrial hemp byproduct, namely hemp seeds, is generated and is used as a high-value food raw material, so that the industrial hemp byproduct contains rich oil and a large amount of high-purity protein, but the utilization of the hemp seeds only stays on the oil, and the industrial hemp seed is not scaled as supplementary non-mainstream edible oil. The nutritional value of hemp seed protein has not been considered sufficiently for a long time, and even after oil extraction it is discarded as waste, only a small part being used in livestock feed.
With the development of scientific technology and the change of nutritional structures of people, researchers gradually break through the research on the hempseeds, the hempseed protein is rich in various essential amino acids, the essential amino acids cannot be synthesized and can only be absorbed through the outside, the hempseed forms micromolecular polypeptide substances after being subjected to the enzymolysis of complex enzyme, the slow wave sleep and 5-hydroxytryptamer synapsin system action mechanism in brainstem can be effectively increased, the sleep-aiding polypeptide substances are generated, and delta waves are induced to induce sleep.
The vegetable protein beverage is an opaque liquid product prepared from seeds, fruits, nuts, kernels and the like of plants with high protein content as raw materials, and the protein content in the finished product is not lower than 0.5% (m/V). Some vegetable protein drinks also appear from hemp seeds, but the quality is uneven, and the following problems are mainly found in many products and patents:
the hemp seed protein powder is directly blended, and the hemp seeds contain protein and a large amount of grease, so that the products are determined to have thermal instability and easy deterioration. The hemp seed protein powder is directly blended, is easy to generate precipitate after being stored for a long time, influences the taste, and is added with a large amount of artificially synthesized additives in the product formula in order to ensure the stability of the product. The unreasonable grinding process causes high grinding plate temperature, easy contamination of bacteria, uneven grinding, plate-forming, hardening and rough taste of the final product.
The hemp seed protein beverage is added with milk powder, milk essence, sodium cyclamate and the like which are mostly non-natural, the flavor of the hemp seed protein beverage is not the original flavor of the hemp seed protein, calcium, vitamins and even hormones are added for increasing functions, and the preparation process is carried out for multiple times of high-temperature treatment, so that the hemp seed protein beverage is neither scientific nor in accordance with the purpose of the invention. It is not suitable for all people, especially for children with protein deficiency and the elderly with weak metabolic function.
Disclosure of Invention
The invention aims to provide a preparation method of a plant protein polypeptide beverage for improving sleep, aims to solve the problems, strives to retain the activity of hemp seed protein to the maximum extent, ensures pure nature, plays the role of the hemp seed protein in improving sleep, can convert hemp seed meal into functional protein food after oil extraction, is comprehensively utilized, is pure nature, has unique flavor and is suitable for large-scale production.
In order to achieve the above purpose, the invention provides the following technical scheme: a preparation method of a plant protein polypeptide beverage for improving sleep comprises the following steps:
the method comprises the following steps: hulling and peeling hemp seeds to obtain seed kernels, grinding the seed kernels into powder, performing Soxhlet extraction by using a solvent, and recovering the solvent to obtain pure defatted hemp seed protein powder;
step two: fully soaking and wetting the hempseed protein powder in the step one by using purified water, grinding into slurry, adding papain and neutral compound protease for enzymolysis in the process of grinding into slurry, carrying out enzyme deactivation and deodorization treatment on the mixed solution after the enzymolysis is finished, and filtering the mixed solution to obtain a hempseed protein polypeptide solution;
step three: and D, adding the hemp seed protein polypeptide liquid in the step II into a natural polymerized sugar flavoring agent and a natural stabilizer, and homogenizing under high pressure to obtain the hemp protein polypeptide beverage.
Further, in the present invention, the method further comprises a fourth step of: canning the hemp protein polypeptide beverage obtained in the third step according to the production specification, and performing pasteurization to obtain the hemp protein polypeptide beverage.
Further, in the invention, in the first step, specifically, hemp seeds with the water content of 5% -10% are subjected to shelling, peeling and seed sorting by a hemp seed sheller, hemp seed kernels are taken and ground into powder, hemp seed kernel powder is obtained and ground into 20-80 meshes, and the weight ratio of the hemp seed kernel powder to the solvent 1: 10-30(m/V) is subjected to Soxhlet extraction at 35-60 ℃, and the solvent is recovered to obtain pure defatted hemp seed protein powder.
Further, in the invention, in the second step, specifically, the pure lipid-removed hemp seed protein powder is mixed according to the weight ratio of 1: 2, fully soaking and wetting the mixture for 2 to 4 hours by using purified water, pulping the mixture by using a pulping machine, controlling the pulping temperature to be 3 to 10 ℃, and controlling the pulping filter screen to be 80 to 150 meshes, wherein the water amount of the purified water is controlled according to the weight of the hemp seed protein powder 1: 2-4 times of the raw materials are synchronously added, 1-4 per thousand of papain and 1-2 per thousand of neutral compound protease are simultaneously added for enzymolysis for 2-4 hours at the temperature of 25-33 ℃, then the raw materials are rapidly heated to 90-95 ℃ under normal pressure for enzyme deactivation, vacuum deodorization is carried out at the temperature of 50-70 ℃, and the hemp seed protein polypeptide liquid is obtained after 200-mesh filtration and 300-mesh filtration.
Further, in the third step of the invention, 1-5% of natural polymeric sugar flavoring agent and 0.5-1% of natural stabilizer are added into the hemp seed protein polypeptide liquid according to the weight ratio, and the mixture is homogenized under high pressure of 80-120Mpa for 30-60min to prepare the hemp protein polypeptide beverage.
Further, in the invention, the solvent is one or more of No. 6 solvent oil, petroleum ether and n-hexane.
Further, in the present invention, the natural polymeric sugar flavor is one or more of isomaltulose, L-arabinose, trehalose, psicose.
The formula of the plant protein polypeptide beverage based on industrial hemp seeds comprises, by weight, 1-5% of a natural flavoring agent, 0.5-1% of a stabilizer, 1-4% of papain hydrolase, 1-2% of neutral compound protease, and the balance of a mixture of defatted hemp seed protein powder and water, wherein the natural flavoring agent comprises, by weight: the water ratio is 1: 4-6, the defatted hemp seed protein powder is prepared by the preparation method of the vegetable protein polypeptide beverage for improving sleep.
The beneficial effects are that the technical scheme of this application possesses following technological effect:
1. the invention can be directly prepared from hemp seeds, and can also be prepared from hemp seed meal after oil extraction, so that hemp seed resources are comprehensively utilized. The defatted hemp seed protein is used for preparing the protein polypeptide liquid, the stability of the protein polypeptide liquid is guaranteed due to the influence of thermal instability of grease on the protein liquid, the pulping temperature is strictly controlled, the protein activity is fully guaranteed due to low-temperature pulping, the original nutritional value of the hemp seed protein is fully exerted, the natural flavoring agent is adopted, the harm of the flavoring agents such as sucralose and the like to intestinal flora is avoided, the addition is more natural and pure, and the protein polypeptide liquid is suitable for various crowds.
2. The invention has simple operation and low equipment cost, adopts mature and reliable cold soaking mode for degreasing, and avoids using supercritical CO2Huge equipment cost and operation safety risk brought by degreasing. The prepared hemp seed protein polypeptide beverage has pure taste, pure nature and unique flavor, and is suitable for large-scale production.
3. The hemp seed protein polypeptide beverage has obvious effect on improving sleep, enriches sleep products and provides a reliable technology and product support for improving sleep quality.
It should be understood that all combinations of the foregoing concepts and additional concepts described in greater detail below can be considered as part of the inventive subject matter of this disclosure unless such concepts are mutually inconsistent.
The foregoing and other aspects, embodiments and features of the present teachings can be more fully understood from the following description taken in conjunction with the accompanying drawings. Additional aspects of the present invention, such as features and/or advantages of exemplary embodiments, will be apparent from the description which follows, or may be learned by practice of specific embodiments in accordance with the teachings of the present invention.
Drawings
The drawings are not intended to be drawn to scale. In the drawings, each identical or nearly identical component that is illustrated in various figures may be represented by a like numeral. For purposes of clarity, not every component may be labeled in every drawing. Embodiments of various aspects of the present invention will now be described, by way of example, with reference to the accompanying drawings, in which:
FIG. 1 is a graph showing the results of the tests of the examples.
Fig. 2 is a graph showing sensory evaluation results of the respective examples.
FIG. 3 is a schematic representation of the nutrient profile (per 100ml) for each example.
Figure 4 is a graph showing the results of the effect of polypeptide fluid on the incidence of subthreshold dose sleep of sodium pentobarbital (n-20).
Fig. 5 is a graph showing the effect of polypeptide solution on sleep latency of barbital sodium (n ═ 20).
Detailed Description
In order to better understand the technical content of the present invention, specific embodiments are described below with reference to the accompanying drawings.
In this disclosure, aspects of the present invention are described with reference to the accompanying drawings, in which a number of illustrative embodiments are shown. Embodiments of the present disclosure are not necessarily intended to include all aspects of the invention. It should be appreciated that the various concepts and embodiments described above, as well as those described in greater detail below, may be implemented in any of numerous ways, as the disclosed concepts and embodiments are not limited to any one implementation. In addition, some aspects of the present disclosure may be used alone, or in any suitable combination with other aspects of the present disclosure.
Example 1:
a preparation method of a polypeptide beverage for improving sleep vegetable protein comprises the following steps:
hulling hemp seeds with the water content of 5 percent by adopting a special hemp seed huller, hulling and sorting the seeds. Grinding hemp seed kernel to 20 meshes, and mixing with No. 6 solvent oil according to the weight ratio of 1: 10(m/V) performing Soxhlet extraction at 40 ℃, and recovering the solvent to obtain pure defatted hemp seed protein powder; the weight ratio of the defatted protein powder is 1: 2, fully soaking and wetting the mixture for 2 hours by using purified water, pulping the mixture by a pulping machine, strictly controlling the pulping temperature to be 3 ℃ and the pulping filter screen to be 80 meshes, and controlling the water amount of the purified water according to the weight of the protein powder to be 1: adding 2 times of the raw materials synchronously, adding 1 ‰ papain and 1 ‰ neutral compound protease, performing enzymolysis at 25 deg.C for 4 hr, rapidly heating to 90 deg.C under normal pressure to inactivate enzyme, vacuum deodorizing at 50 deg.C, and filtering with 300 mesh sieve to obtain hemp seed protein polypeptide solution;
adding 3% isomaltulose and 1% natural stabilizer into the hemp seed protein polypeptide liquid according to weight ratio, homogenizing under 100Mpa for 30min to obtain hemp protein polypeptide beverage, canning according to production specification, and pasteurizing.
Example 2:
the preparation method of the sleep improvement vegetable protein polypeptide beverage specifically comprises the following steps:
hulling hemp seeds with the water content of 10 percent by adopting a special hemp seed huller, hulling and sorting the seeds. Grinding hemp seeds to 80 meshes, and mixing the powder with n-hexane according to the weight ratio of 1: 30(m/V) is subjected to Soxhlet extraction at 60 ℃, and the solvent is recovered to obtain pure defatted hemp seed protein powder; the weight ratio of the defatted protein powder is 1: 2, fully soaking and wetting the mixture for 4 hours by using purified water, pulping the mixture by a pulping machine, strictly controlling the pulping temperature to be 10 ℃ and the pulping filter screen to be 150 meshes, and controlling the water amount of the purified water according to the weight of the protein powder to be 1: adding 4 times of the raw materials synchronously, adding 1 ‰ papain and 2 ‰ neutral compound protease, performing enzymolysis at 33 deg.C for 4 hr, rapidly heating to 95 deg.C under normal pressure to inactivate enzyme, vacuum deodorizing at 70 deg.C, and filtering with 200 mesh sieve to obtain hemp seed protein polypeptide solution; adding 5% natural trehalose and 1% natural stabilizer into the hemp seed protein polypeptide liquid according to weight ratio, and homogenizing under 120Mpa for 60min to obtain the hemp protein polypeptide beverage. The hemp protein polypeptide beverage is canned according to production specifications and is prepared by pasteurization.
Example 3:
the hemp seeds with the water content of 8 percent are hulled, peeled and sorted by a special hemp seed huller. Grinding the hemp seeds into powder, grinding the powder to 40 meshes, and mixing the powder with petroleum ether according to the weight ratio of 1: soxhlet extraction is carried out at the temperature of 45 ℃ at 20(m/V), and the solvent is recovered to obtain pure defatted hemp seed protein powder; the weight ratio of the defatted protein powder is 1: 2, fully soaking and wetting the mixture for 3 hours by using purified water, pulping the mixture by a pulping machine, strictly controlling the pulping temperature to be 10 ℃ and the pulping filter screen to be 100 meshes, and controlling the water amount of the purified water according to the weight of the protein powder to be 1: adding 3 times of the above raw materials simultaneously, adding 2 ‰ papain and 1.5 ‰ of neutral compound protease, performing enzymolysis at 30 deg.C for 3 hr, rapidly heating to 95 deg.C under normal pressure to inactivate enzyme, vacuum deodorizing at 60 deg.C, and filtering with 250 mesh sieve to obtain hemp seed protein polypeptide solution; adding 3% L-arabinose and 0.8% natural stabilizer into the hemp seed protein polypeptide liquid according to the weight ratio, and homogenizing under 100Mpa for 50min to obtain the hemp protein polypeptide beverage. The hemp protein polypeptide beverage is canned according to production specifications and is prepared by pasteurization.
The test results, sensory evaluation results and nutrient composition tables (per 100ml) of examples 1 to 3 are shown in FIGS. 1 to 3.
The embodiment also provides a formula of the plant protein polypeptide beverage based on industrial hemp seeds, which comprises the following components in percentage by weight, 1-5% of natural flavoring agents, 0.5-1% of stabilizing agents, 1-4% of papain hydrolase, 1-2% of neutral compound protease, and the balance of a mixture of defatted hemp seed protein powder and water, and the defatted hemp seed protein powder: the water ratio is 1: 4-6, the defatted hemp seed protein powder is prepared by the preparation method of the vegetable protein polypeptide beverage for improving sleep.
In addition, the invention performs pharmacodynamic evaluation experiments on the sleep improvement effect of the cannabis sativa seed protein polypeptide beverage, and hereinafter, the polypeptide beverage for improving the sleep vegetable protein is collectively referred to as cannabis sativa seed protein polypeptide liquid, specifically as follows.
Experiment: 80 ICR male mice were divided into 4 groups, blank (normal saline) control group, diazepam group, hemp seed protein polypeptide liquid 1, 2 groups. The cannabis seed protein polypeptide solutions 1 and 2 are obtained in examples 1, 2 and 3, each test group is orally administered with 10ml of normal saline, 10ml of diazepam (2.5mg/kg) and 5ml and 10ml of cannabis seed protein polypeptide solution every day, and the effect of the cannabis seed protein polypeptide solution on the sleep latency of mice is evaluated after 30 days by using a direct sleep experiment, a pentobarbital sodium sleep time prolonging experiment, a pentobarbital sodium subliminal dose hypnosis experiment and a barbital sodium sleep period experiment. As shown in fig. 4-5.
The result is that the cannabis sativa seed protein polypeptide liquid is administered for 30 days after gastric administration, and the weight increase of the mice is not influenced; in each group of mice, within 60min after the mice are given a test object, through experimental observation, the polypeptide liquid group and the control group have no direct sleep phenomenon, and the hemp seed protein polypeptide liquid group has no direct sleep promotion effect; in an experiment for prolonging the sleep time of the pentobarbital sodium, compared with a blank control group, the hemp seed protein polypeptide liquid can remarkably prolong the sleep time of the pentobarbital sodium, and the sleep latency of a 10ml hemp seed protein polypeptide liquid group can be remarkably shortened; in the sodium pentobarbital subliminal dose hypnosis experiment, the sleep rate of a 10ml dose group is stronger than that of a diazepam (2.5mg/kg) group, which shows that the composition has synergistic effect on the hypnosis of mice with the sodium pentobarbital subliminal dose; in a barbiturate sodium sleep latency experiment, the sleep latency of a cannabis sativa seed protein polypeptide liquid group of 5ml and 10ml is extremely obviously shortened, and the sleep latency of the cannabis sativa seed protein polypeptide liquid group of 10ml is extremely shorter than that of a positive control group.
The conclusion is that the hemp seed protein polypeptide liquid has the function of improving sleep. The mechanism of improving sleep is further studied.
The invention has the following beneficial effects:
the invention can be directly prepared from hemp seeds or can be prepared from hemp seed meal after oil extraction, so that hemp seed resources are comprehensively utilized.
The defatted hemp seed protein is used for preparing the protein polypeptide liquid, the stability of the protein polypeptide liquid is guaranteed due to the influence of thermal instability of grease on the protein liquid, the pulping temperature is strictly controlled, the protein activity is fully guaranteed due to low-temperature pulping, the original nutritional value of the hemp seed protein is fully exerted, the natural flavoring agent is adopted, the harm of the flavoring agents such as sucralose and the like to intestinal flora is avoided, the addition is more natural and pure, and the protein polypeptide liquid is suitable for various crowds.
The invention has simple operation and low equipment cost, adopts mature and reliable cold soaking mode for degreasing, and avoids using supercritical CO2Huge equipment cost and operation safety risk brought by degreasing. The prepared hemp seed protein polypeptide beverage has pure taste, pure nature and unique flavor, and is suitable for large-scale production.
The hemp seed protein polypeptide beverage has obvious effect on improving sleep, enriches sleep products and provides a reliable technology and product support for improving sleep quality.
Although the present invention has been described with reference to the preferred embodiments, it is not intended to be limited thereto. Those skilled in the art can make various changes and modifications without departing from the spirit and scope of the invention. Therefore, the protection scope of the present invention should be determined by the appended claims.
Claims (8)
1. A preparation method of a plant protein polypeptide beverage for improving sleep is characterized by comprising the following steps: the method comprises the following steps:
the method comprises the following steps: hulling and peeling hemp seeds to obtain seed kernels, grinding the seed kernels into powder, performing Soxhlet extraction by using a solvent, and recovering the solvent to obtain pure defatted hemp seed protein powder;
step two: fully soaking and wetting the hempseed protein powder in the step one by using purified water, grinding into slurry, adding papain and neutral compound protease for enzymolysis in the process of grinding into slurry, carrying out enzyme deactivation and deodorization treatment on the mixed solution after the enzymolysis is finished, and filtering the mixed solution to obtain a hempseed protein polypeptide solution;
step three: and D, adding the hemp seed protein polypeptide liquid in the step II into a natural polymerized sugar flavoring agent and a natural stabilizer, and homogenizing under high pressure to obtain the hemp protein polypeptide beverage.
2. The method for preparing a plant protein polypeptide beverage for improving sleep according to claim 1, wherein the plant protein polypeptide beverage comprises the following components: the method also comprises the following four steps: canning the hemp protein polypeptide beverage obtained in the third step according to the production specification, and performing pasteurization to obtain the hemp protein polypeptide beverage.
3. The method for preparing a plant protein polypeptide beverage for improving sleep according to claim 1, wherein the plant protein polypeptide beverage comprises the following components: specifically, the hemp seeds with the water content of 5% -10% are subjected to shelling, peeling and seed sorting by a hemp seed sheller, the hemp seeds are ground into powder to obtain hemp seed powder, the powder is ground into 20-80 meshes, and the weight ratio of the hemp seed powder to the solvent 1 is as follows: 10-30(m/V) is subjected to Soxhlet extraction at 35-60 ℃, and the solvent is recovered to obtain pure defatted hemp seed protein powder.
4. The preparation method of the plant protein polypeptide beverage for improving sleep according to claim 3, wherein the plant protein polypeptide beverage comprises the following steps: in the second step, specifically, the hemp seed protein powder without pure fat is mixed according to the weight ratio of 1: 2, fully soaking and wetting the mixture for 2 to 4 hours by using purified water, pulping the mixture by using a pulping machine, controlling the pulping temperature to be 3 to 10 ℃, and controlling the pulping filter screen to be 80 to 150 meshes, wherein the water amount of the purified water is controlled according to the weight of the hemp seed protein powder 1: 2-4 times of the raw materials are synchronously added, 1-4 per thousand of papain and 1-2 per thousand of neutral compound protease are simultaneously added for enzymolysis for 2-4 hours at the temperature of 25-33 ℃, then the raw materials are rapidly heated to 90-95 ℃ under normal pressure for enzyme deactivation, vacuum deodorization is carried out at the temperature of 50-70 ℃, and the hemp seed protein polypeptide liquid is obtained after 200-mesh filtration and 300-mesh filtration.
5. The preparation method of the plant protein polypeptide beverage for improving sleep according to claim 4, wherein the plant protein polypeptide beverage comprises the following steps: in the third step, 1-5% of natural polymerized sugar flavoring agent and 0.5-1% of natural stabilizer are added into the hemp seed protein polypeptide liquid according to the weight ratio, and the mixture is homogenized under high pressure of 80-120Mpa for 30-60min to prepare the hemp protein polypeptide beverage.
6. The preparation method of the plant protein polypeptide beverage for improving sleep according to claim 3, wherein the plant protein polypeptide beverage comprises the following steps: the solvent is one or more of No. 6 solvent oil, petroleum ether and n-hexane.
7. The method for preparing a plant protein polypeptide beverage for improving sleep according to claim 5, wherein the plant protein polypeptide beverage comprises the following components: the natural polymeric sugar flavoring agent is one or more of isomaltulose, L-arabinose, trehalose, and psicose.
8. The formula of the industrial hemp seed-based vegetable protein polypeptide beverage is prepared from the following components in percentage by weight: 1-5% of natural flavoring agent, 0.5-1% of stabilizer, 1-4% of papain hydrolase, 1-2% of neutral compound protease, and the balance of mixture of defatted hemp seed protein powder and water, defatted hemp seed protein powder: the water ratio is 1: 4-6, the defatted hemp seed protein powder is prepared by the preparation method of the plant protein polypeptide beverage for improving sleep as claimed in any one of claims 1-7.
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| CN116172208A (en) * | 2022-08-17 | 2023-05-30 | 广东医科大学 | Psicose composition for promoting sleep and preparation method thereof |
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