CN103976372A - Preparation method of ponkan dietary fiber - Google Patents
Preparation method of ponkan dietary fiber Download PDFInfo
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- CN103976372A CN103976372A CN201410242955.8A CN201410242955A CN103976372A CN 103976372 A CN103976372 A CN 103976372A CN 201410242955 A CN201410242955 A CN 201410242955A CN 103976372 A CN103976372 A CN 103976372A
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 24
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- 241001149649 Taxus wallichiana var. chinensis Species 0.000 description 2
- LUKBXSAWLPMMSZ-OWOJBTEDSA-N Trans-resveratrol Chemical compound C1=CC(O)=CC=C1\C=C\C1=CC(O)=CC(O)=C1 LUKBXSAWLPMMSZ-OWOJBTEDSA-N 0.000 description 2
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- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- QUQPHWDTPGMPEX-UTWYECKDSA-N aurantiamarin Natural products COc1ccc(cc1O)[C@H]1CC(=O)c2c(O)cc(O[C@@H]3O[C@H](CO[C@@H]4O[C@@H](C)[C@H](O)[C@@H](O)[C@H]4O)[C@@H](O)[C@H](O)[C@H]3O)cc2O1 QUQPHWDTPGMPEX-UTWYECKDSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
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- 230000033228 biological regulation Effects 0.000 description 1
- 239000012267 brine Substances 0.000 description 1
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- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000001071 citrus reticulata blanco var. mandarin Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
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- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
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- 239000003925 fat Substances 0.000 description 1
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- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
- A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
- A23L33/22—Comminuted fibrous parts of plants, e.g. bagasse or pulp
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
- A23L19/03—Products from fruits or vegetables; Preparation or treatment thereof consisting of whole pieces or fragments without mashing the original pieces
- A23L19/07—Fruit waste products, e.g. from citrus peel or seeds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/30—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation
- A23L5/32—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation using phonon wave energy, e.g. sound or ultrasonic waves
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention belongs to the technical field of fruit and vegetable processing and citrus comprehensive utilization and in particular relates to a preparation method of a ponkan dietary fiber. The preparation method comprises the steps of with ponkan peel pomace as a raw material, carrying out blanching, drying, crushing and ultrasonic-assisted water (microwave assisted water) extraction, carrying out enzymatic hydrolysis, enzyme killing and centrifugation by regulating a pH value and compound enzyme to obtain a filtrate and precipitates, carrying out concentration, alcohol precipitation, alcohol washing, centrifugation and vacuum drying on the filtrate to obtain a water-soluble dietary fiber, and carrying out washing, centrifugation and vacuum drying on the precipitates to obtain a insoluble dietary fiber. With the adoption of the preparation method, with the waste ponkan peel pomace after juicing as the raw material, deep processing of agricultural byproducts raw materials is realized, the resource waste is reduced, and meanwhile, a novel way is provided to comprehensively utilize a ponkan byproduct.
Description
Technical field
The invention belongs to garden stuff processing and oranges and tangerines comprehensive utilization technique field, particularly a kind of preparation method of Pon mandarin orange dietary fiber.
Background technology
Pon mandarin orange is good wide skin Citrus Cultivars, has become at present the topmost fruit variety in the western Hunan, and cultivated area reaches more than 650,000 mu, and output is Yued 500,000 tons.When cultivation industry develops rapidly, the oranges and tangerines deep processing of China is slower development but.To squeeze the juice, be processed as example, after processing, residue skin slag is its Main By product, and its weight accounts for the 30-40% that fruit is heavy, but China's citrus peel residue comprehensive utilization ratio is very low, less than 10%, and the overwhelming majority is simply discarded.Domestic report extracted mandarin oil, pectin, aurantiamarin, food coloring and produced orange peel beverage etc. and utilize approach from orange peel in recent years, but that these utilize approach to have product is single, the shortcoming such as industrialization level is low, and raw material availability is not high, and secondary pollution is serious.Therefore, oranges and tangerines processing byproduct should be avoided environmental pollution to greatest extent as the with different levels reasonable comprehensive utilization of raw material, is the needs that meet the sustainable high-efficient development of human resource.In the Pon mandarin orange of take processing, the skin slag of accessory substance is raw material; adopt advanced practical science and technology; make full use of and preserve the functional health ingredient of Pon orange peel slag; prepare high-quality Pon orange peel dregs diet fibre; not only realized zero in the processing of Pon mandarin orange and discarded, resource has obtained adequately and reasonably utilizing, and has protected ecological environment; to optimizing Citrus Industry structure, the added value tool that increases oranges and tangerines processing industry is of great significance.
Dietary fiber (dietary fiber, DF) refers to not the SNSP digested and assimilated by stomach and small intestine and the general name of lignin one family macromolecule carbohydrate.Although dietary fiber can not provide any nutrition for human body, but human body is had to important physiological function---dietary fiber has angiocardiopathy preventing, obesity, diabetes, hypertension, improve flora function in enteron aisle, reduce gastric and intestinal cancer occur, remove external source harmful substance, anti-oxidant, remove the physiological effects such as free radical, be called as " the 7th class nutrient " after carbohydrate, protein, fat, vitamin, mineral matter and water.
By dietary fiber dissolubility, can be divided into water-soluble fibre and the large class of insoluble fibre two.Stored substance and secretion in the main phalangeal cell wall of water-soluble dietary fiber, as pectin, part hemicellulose and natural plant gum etc.; The part of the main phalangeal cell wall of insoluble diedairy fiber, as cellulose, part hemicellulose, lignin, shitosan and vegetable wax etc.
The method of extracting at present dietary fiber both at home and abroad mainly contains chemical leaching test, enzyme extraction method, chemistry-enzyme in conjunction with extraction method, membrane separation process and fermentation method.The method of dietary fiber being carried out to modification has: biological technique method (enzyme process and fermentation method), chemical treatment method (mainly containing acid system, alkaline process) and physical method (mechanical degradation facture).These methods are all single, independently, lack effective combination, cause recovery rate low, and Product Activity is low, or operation is too complicated, is unfavorable for realizing industrialization.Therefore, be badly in need of a kind of method that can high efficiency extraction dietary fiber of high activity, can extract and the effective combination of modification, obtain meeting the dietary fiber of high activity product of human body needs.
It is that the active ingredient of plant is extracted in the strengthening of using ultrasound ripple that ultrasonic wave extracts, and is a kind of physics shattering process.Ultrasonic wave mainly produces unique mechanical oscillation effect and cavitation effect to material.When ultrasonic vibration, can produce and transmit powerful energy, cause that material particle enters vibrational state with larger speed and acceleration, material structure is changed, impel active ingredient to enter in solvent; In liquid, also can produce cavitation,, under the effect that has sizable bursting stress, in liquid, form the phenomenon of cavitation bubble simultaneously.The patent of publication number CN103122037A discloses a kind of method that ultrasonic wave extracts LBP-X, and it obtains recovery rate through ultrasonic wave extraction, filtration, alcohol precipitation, vacuum drying is 7.03% LBP-X; The patent of publication number CN102430041A discloses the method for extracting total saponins of cochinchinese asparagus root by using ultrasonic waves, studied the impact of ultrasonic wave extraction conditions on asparagus fern total saponin and purity, after immersion, ultrasonic extraction, concentrated, degreasing, the product that obtain recovery rate and be 3.371%, purity is 90.3%; The patent of publication number CN102491880A discloses the technique of extracting resveratrol and pigment from Grape Skin, adopt solvent pairs and ultrasonic extraction integrated, through organic solvent dissolution, ultrasonic wave extraction, macroreticular resin and silica gel column chromatography, carry out after separation and purification, through vacuum drying, obtain resveratrol and Grape Skin anthocyanidin powder, its recovery rate is respectively 1.2% and 22.9%, and purity all reaches more than 95%.
Microwave is the electromagnetic wave of frequency between 300MHz and 300GHz, heating using microwave is to move by heated object internal dipole molecule high-frequency reciprocating, produce " interior frictional heat " and make to be heated temperature of charge rising, must any heat transfer process, just can make material inside and outside portion heat simultaneously, heat up, firing rate is fast and even simultaneously.Because microwave has strong, selective high, the extraction efficiency high of penetration power, thereby be widely used in the fields such as active components of plants extraction, food drying, sterilizing.The patent of publication number CN101475649A discloses the extraction process of coral fungi polysaccharide, and after pretreatment, the coral fungi polysaccharide that carry out Microwave Extraction, concentrated, alcohol precipitation, obtains after centrifugal, dry is 70% to the inhibiting rate of small white mouse S-180, ehrlich carcinoma to raw material; The patent of publication number CN101475650A discloses the extraction process of Hericium erinaceus polysaccharide, adopts Microwave Extraction method to save extraction time, has improved efficiency, and extract has immunological regulation, antitumor, the anti-ageing effect of waiting for a long time.
At present domestic to the report that extracts water-soluble dietary fiber and insoluble diedairy fiber in skin slag simultaneously seldom, especially the patent of Pon mandarin orange dietary fiber is reported not yet to some extent.The patent of publication number CN102885318A discloses a kind of extracting method of Rhizoma Dioscoreae esculentae dietary fiber, take the separated soluble dietary fiber of the compound preparation of AMS, trypsase and carbohydrase and insoluble diedairy fiber, and studied the impact of enzymatic hydrolysis condition on product yield, recovery rate is respectively 25.7% and 55.9%; The patent of publication number CN103284152A discloses a kind of dietary fiber extracting from Chinese yew, extracting method and purposes, adopt high-pressure homogeneous-enzyme combined techniques to extract soluble dietary fiber and insoluble diedairy fiber, obtain the Chinese yew insoluble diedairy fiber (80%-90%) of higher yield, but the content of its soluble dietary fiber lower (10%-13%); The patent of publication number CN102871134A discloses a kind of method of extracting dietary fiber, after brine electrolysis extracts, heating water bath, cooling rear centrifugal, concentrated, alcohol precipitation, obtain dregs of beans solubility, insoluble diedairy fiber after dry, content is respectively 14.8% and 53.7%; The patent of publication number CN103190632A discloses a kind of preparation method of edible sea-buckthorn dietary fiber, after seed residues of seabuckthorn after supercritical carbon dioxide degreasing mixes with water, protease and cellulase complex enzyme hydrolysis, Vacuum Concentration, alcohol precipitation, dry seed residues of seabuckthorn soluble dietary fiber and the insoluble diedairy fiber of obtaining of spraying, soluble dietary fiber yield is higher than 30%, insoluble diedairy fiber yield reaches more than 50%, and insoluble diedairy fiber has higher retention ability and expansive force.
Summary of the invention
The object of the present invention is to provide a kind of preparation method of Pon mandarin orange dietary fiber, obtain two kinds of dietary fiber of high activity products simultaneously.
For prior art deficiency, the invention provides a kind of preparation method of Pon mandarin orange dietary fiber.
A preparation method for Pon mandarin orange dietary fiber, obtains soluble dietary fiber and insoluble diedairy fiber product through the method, and concrete steps are as follows:
(1) pretreatment of raw material: by Pon orange peel slag hot water blanching to be processed; Its effect enzyme (PPO enzymatic activity) that goes out, suppresses enzymatic browning.Pon orange peel slag after blanching is dried, pulverizes sieving for standby;
(2) add aqueous dispersion: the Pon orange peel slag after step (1) is pulverized is added to aqueous dispersion, stir;
(3) Pon orange peel slag lixiviate: carry out lixiviate by ultrasonic wave auxiliary water extraction or using microwave assisted aqueous extraction extraction;
A kind of physics shattering process of ultrasonic wave, can produce and transmit powerful energy during vibration, causes that material particle enters vibrational state with larger speed and acceleration, and material structure is changed, and impels soluble dietary fiber to enter in solvent;
Microwave treatment makes to be heated temperature of charge and raises, must any heat transfer process, just can make material inside and outside portion heat simultaneously, heat up simultaneously, firing rate is fast and even, microwave has strong, selective high, the extraction efficiency high of penetration power simultaneously, can effectively be applied to the extraction of soluble dietary fiber;
(4) complex enzyme carries out enzymolysis: gained leaching liquor in step (3) is regulated to pH with HCl solution, add successively cellulase, pectin lyase, 1,4 beta-glucanase to carry out enzymolysis, obtain enzymolysis liquid.The combination of cellulase and pectin lyase, promote the cell of fruit cortex decompose and come off, cellulose in degradation of cell wall, hemicellulose, make cell wall expansion, conversion, make cellulose substances degraded, destroy plant cell wall, make dissolution of cellular content to discharge interior nutrient, be conducive to promote insoluble diedairy fiber to the conversion of soluble dietary fiber simultaneously.In the specific hydrolysis beta glucan of 1,4 beta-glucanase energy 1,3-or Isosorbide-5-Nitrae-wait glycosidic bond, make it to be degraded to low molecular sugar, destroy the structure of plant cell wall, thereby discharged nutriment.
(5) enzyme that goes out: step (4) the gained enzymolysis liquid enzyme that goes out is processed;
(6) centrifugal treating: step (5) the gained solution after enzyme that goes out is clarified by centrifugal separation process, be precipitated thing and supernatant;
(7) washing is processed: will after step (6) is centrifugal, wash processing by gained sediment, centrifugation obtains insoluble diedairy fiber sediment; Washing repeatedly improves the purity of insoluble diedairy fiber.
(8) concentrated alcohol wash, alcohol precipitation, processing: the centrifugal rear gained supernatant of step (6) is carried out to concentration, obtain concentrate.Gained concentrate carries out alcohol precipitation processing, and centrifugation obtains alcohol precipitation sediment, then carries out alcohol wash processing, and centrifugation obtains soluble dietary fiber sediment;
(9) vacuum drying treatment: step (7) and step (8) gained insoluble diedairy fiber sediment and soluble dietary fiber sediment are placed in to vacuum drying chamber dry, obtain insoluble diedairy fiber and soluble dietary fiber;
(10) preservation processing: insoluble diedairy fiber and soluble dietary fiber through the dry gained of step (9) are ground into after fine powder, pack the interior sealing preservation of aluminum foil sack into or carry out product processing.
The skin slag of Pon orange peel slag in described step (1) for obtaining after the Pon mandarin orange fresh fruit after cleaning is squeezed the juice, its heat iron temperature is 85 ℃～100 ℃, blanching treatment time is 1min～3min.After Pon orange peel slag draining after blanching, put into the dry 24h～48h of 50 ℃～70 ℃ electric heating constant-temperature blowing drying boxes, being dried to water content is 4%-7%, is crushed to 60～80 orders, obtains Pon orange peel ground-slag end.
Pon orange peel slag after pulverizing in described step (2) and the mixing ratio of water are 1g:(3mL～5mL).
The extraction temperature of the ultrasonic auxiliary water lixiviate in described step (3) is 40 ℃～80 ℃, and solid-to-liquid ratio is 1g:(10mL～25mL), extraction time is 20min～60min, and ultrasonic power is 300W～500W, and dutycycle is 1:2, extracts 1～2 time.
Solid-to-liquid ratio 1g:(10mL～the 25mL of the using microwave assisted aqueous extraction lixiviate in described step (3)), extraction time is 0.5min～10min, microwave power 300W～700W, and microwave temperature is 50 ℃～90 ℃, extracts 1～2 time.
The enzyme work of described cellulase is defined as: 1min decomposes the required enzyme amount of generation 1 μ mol reduced sugar from substrate solution, and 1 Ge Meihuo unit, represents with U/mL; The enzyme of described step (4) cellulase is lived as 300U/mL～400U/mL; The enzyme work of described pectin lyase is defined as: decompose pectin per minute, and make 235nm place extinction value increase by 1.0 enzyme amount, be defined as 1 Ge Meihuo unit, with U/mL, represent; In described step (4), the enzyme of pectin lyase is lived as 400U/mL～500U/mL; The enzyme work of described 1,4 beta-glucanase is defined as: the beta glucan solution that 1g solid enzyme powder or 1mL enzyme liquid 1min are 5mg/mL from concentration, the required enzyme amount of degraded release 1 μ mol reduced sugar is defined as 1 Ge Meihuo unit, with U/mL, represents; In described step (4), the enzyme of 1,4 beta-glucanase is lived as 5000U/mL～10000U/mL.
The HCl solution that is 0.5mol/L by concentration in described step (4) regulates pH value to 3.5～5.5, adding cellulase addition is 1.0%～4.0% of leaching liquor quality, be placed in 50 ℃～70 ℃ water bath with thermostatic control shaking tables, rotating speed is 100r/min～120r/min, and enzymolysis time is 6h～10h; The addition of pectin lyase is 0.15%～0.30% of leaching liquor quality, at 30 ℃～60 ℃ temperature, stirs enzymolysis 30min～80min; The addition of 1,4 beta-glucanase is 0.05%～0.20% of leaching liquor quality, under 30 ℃～60 ℃ conditions, stirs enzymolysis 25min～60min.
In described step (5), by step (4) gained enzymolysis liquid, at 95 ℃ of enzyme 2min that go out, be cooled to room temperature.
In described step (6), step (7) and step (8), centrifugal rotational speed is respectively 4000r/min～4200r/min, and centrifugation time is 20min～30min.
In described step (7), by the centrifugal rear gained sediment washing of step (6) 1～3 time, solid-to-liquid ratio is 1g:(5mL～10mL), washing temperature is 50 ℃～80 ℃, centrifugation, obtains insoluble diedairy fiber sediment.
In described step (8), the centrifugal rear gained supernatant of step (6), at 50 ℃～60 ℃, is rotated to evaporation and concentration to 1/7～1/15 of original volume under the vacuum of 0.09MPa～0.1MPa, obtain concentrate.The ethanol that is 55%～95% to the volume fraction of adding 3-7 times of concentrate volume in gained concentrate carries out alcohol precipitation, and the alcohol precipitation time is 4h～12h, and alcohol precipitation temperature is 20 ℃～40 ℃, and centrifugation obtains alcohol precipitation sediment.Sediment carries out alcohol wash processing, and ethanol mass fraction used is 55%～95%, and alcohol wash number of times is 1～3 time, and alcohol wash temperature is 20 ℃～40 ℃, obtains soluble dietary fiber sediment.
In described step (9), vacuum drying temperature is 40 ℃～60 ℃, and vacuum is 0.6MPa～0.9MPa, and be 2h～5h drying time.
Beneficial effect of the present invention is:
(1) take discarded skin slag in the processing of Pon mandarin orange is raw material, reduces the wasting of resources; (2) adopt not only environmental protection but also comparatively simple and easy, feasible method to prepare Pon mandarin orange dietary fiber, less demanding to production operation and equipment; (3) extracting method of ultrasonic wave and enzymolysis coupling is to utilize ultrasonic wave to have cavitation effect, mechanical effect and fuel factor, these effects have increased the movement velocity of medium molecule, improve the penetration capacity of medium, promote active ingredient to dissolve and diffusion, shorten extraction time, improved the recovery rate of water-soluble dietary fiber, change the Nomenclature Composition and Structure of Complexes of dietary fiber, strengthened its adsorption capacity; The extracting method firing rate of microwave and enzymolysis coupling is fast, and the heated time of material is short, also can under lower temperature conditions, complete extracting operation, so can effectively protect the functional component of dietary fiber, has the feature of energy-saving and environmental protection.
Accompanying drawing explanation
Fig. 1 is the process chart that the present invention prepares Pon mandarin orange dietary fiber.
The specific embodiment
The preparation method who the invention provides a kind of Pon mandarin orange dietary fiber, below in conjunction with the drawings and specific embodiments, the present invention will be further described.
Embodiment 1
(1) pretreatment of raw material: the Pon orange peel slag obtaining after the Pon mandarin orange fresh fruit after cleaning is squeezed the juice carries out blanching processing, and heat iron temperature is 95 ℃, and blanching treatment time is 1.5min.Gained Pon orange peel slag after blanching, puts into the dry 36h of 60 ℃ of electric heating constant-temperature blowing drying boxes, and its water content is 5%, is crushed to 80 orders, obtains pretreated Pon orange peel ground-slag end.
(2) add aqueous dispersion: the Pon orange peel slag after step (1) is pulverized is added to aqueous dispersion, and solid-to-liquid ratio is 1g:3mL, stirs, and makes dispersion liquid;
(3) ultrasonic auxiliary water lixiviate: in step (2), dispersion liquid is positioned in ultrasonic leaching device, and extraction temperature is 55 ℃, and solid-to-liquid ratio is 1g～15mL, extraction time is 30min, and ultrasonic power is 500W, and dutycycle is 1:2, extract 2 times, obtain leaching liquor;
(4) complex enzyme carries out enzymolysis: the HCl solution that is 0.5mol/L by concentration to step (3) gained leaching liquor regulates pH value to 5.0, adds successively cellulase, pectin lyase, 1,4 beta-glucanase to carry out enzymolysis, obtains enzymolysis liquid; Cellulase addition is 2.0% of leaching liquor quality, is placed in 60 ℃ of water bath with thermostatic control shaking tables, and rotating speed is 120r/min, and enzymolysis time is 6h; The addition of pectin lyase is 0.20% of leaching liquor quality, at 40 ℃ of temperature, stirs enzymolysis 45min; The addition of 1,4 beta-glucanase is 0.10% of leaching liquor quality, at 35 ℃ of temperature, stirs enzymolysis 30min;
(5) enzyme that goes out: by gained enzymolysis liquid in step (4), at 95 ℃ of enzyme 2min that go out, be cooled to room temperature;
(6) centrifugal treating: the solution that gained in step (5) is gone out after enzyme carries out centrifugal, and rotating speed is 4200r/min, and the time is 25min, is precipitated thing and supernatant;
(7) washing is processed: will after step (6) is centrifugal, wash 3 times by gained sediment, solid-to-liquid ratio is 1g:9mL, and washing temperature is 65 ℃, and centrifugation obtains insoluble diedairy fiber sediment;
(8) concentrated, alcohol precipitation, alcohol wash are processed: will after step (6) is centrifugal, at 60 ℃, rotate evaporation and concentration to 1/12 of original volume under the vacuum of 0.09MPa for gained supernatant, and obtain concentrate.The ethanol that is 95% to the volume fraction of adding 4 times of concentrate volumes in gained concentrate carries out alcohol precipitation, and alcohol precipitation temperature is 25 ℃, and the alcohol precipitation time is 8h, and centrifugation obtains alcohol precipitation sediment.Sediment carries out alcohol wash processing, and concentration of alcohol used is 75%, and alcohol wash number of times is 2 times, and alcohol wash temperature is 25 ℃, and centrifugation obtains water-soluble dietary fiber sediment;
(9) dry processing: gained sediment in step (7) and step (8) is placed in to vacuum drying chamber dry, baking temperature is 50 ℃, and vacuum is 0.9Mpa, and be 3h drying time, obtains insoluble diedairy fiber and soluble dietary fiber;
(10) preserve: insoluble diedairy fiber and soluble dietary fiber through the dry gained of step (9) are ground into after fine powder, pack the interior sealing preservation of aluminum foil sack into or carry out product processing.
The recovery rate that obtains soluble dietary fiber and insoluble diedairy fiber under this condition is respectively 25.94% and 46.93%, and purity is respectively 91.22% and 72.93%.The cholesterol adsorption capacity of soluble dietary fiber is 29.72mg/g (pH=2.0), 33.84mg/g (pH=7.0), and sodium taurocholate adsorption capacity is 498.32mg/g.The retention ability of insoluble diedairy fiber is 9.34g/g, and holding oily power is 7.21g/g, and swellability is 8.92mL/g.
Embodiment 2
(1) pretreatment of raw material: the Pon orange peel slag obtaining after the Pon mandarin orange fresh fruit after cleaning is squeezed the juice carries out blanching processing, and heat iron temperature is 90 ℃, and blanching treatment time is 2min.Gained Pon orange peel slag after blanching, puts into the dry 36h of 60 ℃ of electric heating constant-temperature blowing drying boxes, and its water content is 5%, is crushed to 80 orders, obtains pretreated Pon orange peel ground-slag end.
(2) add aqueous dispersion: the Pon orange peel slag after step (1) is pulverized is added to aqueous dispersion, and solid-to-liquid ratio is 1g:5mL, stirs, and makes dispersion liquid;
(3) ultrasonic auxiliary water lixiviate: in step (2), dispersion liquid is positioned in ultrasonic leaching device, and extraction temperature is 70 ℃, and solid-to-liquid ratio is 1g～25mL, extraction time is 40min, and ultrasonic power is 450W, and dutycycle is 1:2, extract 2 times, obtain leaching liquor;
(4) complex enzyme carries out enzymolysis: the HCl solution that is 0.5mol/L by concentration to step (3) gained leaching liquor regulates pH value to 4.5, adds successively cellulase, pectin lyase, 1,4 beta-glucanase to carry out enzymolysis, obtains enzymolysis liquid; Cellulase addition is 4.0% of leaching liquor quality, is placed in 55 ℃ of water bath with thermostatic control shaking tables, and rotating speed is 120r/min, and enzymolysis time is 5h; The addition of pectin lyase is 0.30% of leaching liquor quality, at 50 ℃ of temperature, stirs enzymolysis 60min; The addition of 1,4 beta-glucanase is 0.15% of leaching liquor quality, at 45 ℃ of temperature, stirs enzymolysis 50min;
(5) enzyme that goes out: by gained enzymolysis liquid in step (4), at 95 ℃ of enzyme 2min that go out, be cooled to room temperature;
(6) centrifugal treating: the solution that gained in step (5) is gone out after enzyme carries out centrifugal, and rotating speed is 4200r/min, and the time is 25min, is precipitated thing and supernatant;
(7) washing is processed: will after step (6) is centrifugal, wash 3 times by gained sediment, solid-to-liquid ratio is 1g:8mL, and washing temperature is 50 ℃, and centrifugation obtains insoluble diedairy fiber sediment;
(8) concentrated, alcohol precipitation, alcohol wash are processed: will after step (6) is centrifugal, at 50 ℃, rotate evaporation and concentration to 1/10 of original volume under the vacuum of 0.09MPa for gained supernatant, and obtain concentrate.The ethanol that is 95% to the volume fraction of adding 4 times of concentrate volumes in gained concentrate carries out alcohol precipitation, and alcohol precipitation temperature is 30 ℃, and the alcohol precipitation time is 10h, and centrifugation obtains alcohol precipitation sediment.Sediment carries out alcohol wash processing, and concentration of alcohol used is 80%, and alcohol wash number of times is 2 times, and alcohol wash temperature is 35 ℃, and centrifugation obtains water-soluble dietary fiber sediment;
(9) dry processing: gained sediment in step (7) and step (8) is placed in to vacuum drying chamber dry, baking temperature is 60 ℃, and vacuum is 0.9Mpa, and be 2.5h drying time, obtains insoluble diedairy fiber and soluble dietary fiber;
(10) preserve: insoluble diedairy fiber and soluble dietary fiber through the dry gained of step (9) are ground into after fine powder, pack the interior sealing preservation of aluminum foil sack into or carry out product processing.
The recovery rate that obtains soluble dietary fiber and insoluble diedairy fiber under this condition is respectively 26.72% and 44.12%, and purity is respectively 92.01% and 74.91%.The cholesterol adsorption capacity of soluble dietary fiber is 30.21mg/g (pH=2.0), 36.32mg/g (pH=7.0), and sodium taurocholate adsorption capacity is 502.12mg/g.The retention ability of insoluble diedairy fiber is 10.19g/g, and holding oily power is 6.98g/g, and swellability is 8.91mL/g.
Embodiment 3
(1) pretreatment of raw material: the Pon orange peel slag obtaining after the Pon mandarin orange fresh fruit after cleaning is squeezed the juice carries out blanching processing, and heat iron temperature is 85 ℃, and blanching treatment time is 3min.Gained Pon orange peel slag after blanching, puts into the dry 36h of 60 ℃ of electric heating constant-temperature blowing drying boxes, and its water content is 5%, is crushed to 80 orders, obtains pretreated Pon orange peel ground-slag end.
(2) add aqueous dispersion: the Pon orange peel slag after step (1) is pulverized is added to aqueous dispersion, and solid-to-liquid ratio is 1g:4mL, stirs, and makes dispersion liquid;
(3) using microwave assisted aqueous extraction lixiviate: in step (2), dispersion liquid is positioned in microwave leaching device, and extraction temperature is 80 ℃, and solid-to-liquid ratio is 1g～15mL, and extraction time is 8min, and microwave power is 650W, extracts 2 times, obtains leaching liquor;
(4) complex enzyme carries out enzymolysis: the HCl solution that is 0.5mol/L by concentration to step (3) gained leaching liquor regulates pH value to 4.0, adds successively cellulase, pectin lyase, 1,4 beta-glucanase to carry out enzymolysis, obtains enzymolysis liquid; Cellulase addition is 3.0% of leaching liquor quality, is placed in 50 ℃ of water bath with thermostatic control shaking tables, and rotating speed is 120r/min, and enzymolysis time is 8h; The addition of pectin lyase is 0.25% of leaching liquor quality, at 45 ℃ of temperature, stirs enzymolysis 50min; The addition of 1,4 beta-glucanase is 0.20% of leaching liquor quality, at 30 ℃ of temperature, stirs enzymolysis 60min;
(5) enzyme that goes out: by gained enzymolysis liquid in step (4), at 95 ℃ of enzyme 2min that go out, be cooled to room temperature;
(6) centrifugal treating: the solution that gained in step (5) is gone out after enzyme carries out centrifugal, and rotating speed is 4200r/min, and the time is 25min, is precipitated thing and supernatant;
(7) washing is processed: will after step (6) is centrifugal, wash 2 times by gained sediment, solid-to-liquid ratio is 1g:6mL, and washing temperature is 70 ℃, and centrifugation obtains insoluble diedairy fiber sediment;
(8) concentrated, alcohol precipitation, alcohol wash are processed: will after step (6) is centrifugal, at 55 ℃, rotate evaporation and concentration to 1/8 of original volume under the vacuum of 0.09MPa for gained supernatant, and obtain concentrate.The ethanol that is 95% to the volume fraction of adding 7 times of concentrate volumes in gained concentrate carries out alcohol precipitation, and alcohol precipitation temperature is 25 ℃, and the alcohol precipitation time is 5h, and centrifugation obtains alcohol precipitation sediment.Sediment carries out alcohol wash processing, and concentration of alcohol used is 85%, and alcohol wash number of times is 3 times, and alcohol wash temperature is 30 ℃, and centrifugation obtains water-soluble dietary fiber sediment;
(9) dry processing: gained sediment in step (7) and step (8) is placed in to vacuum drying chamber dry, baking temperature is 50 ℃, and vacuum is 0.9Mpa, and be 5h drying time, obtains insoluble diedairy fiber and soluble dietary fiber;
(10) preserve: insoluble diedairy fiber and soluble dietary fiber through the dry gained of step (9) are ground into after fine powder, pack the interior sealing preservation of aluminum foil sack into or carry out product processing.
The recovery rate that obtains soluble dietary fiber and insoluble diedairy fiber under this condition is respectively 25.11% and 45.93%, and purity is respectively 90.32% and 73.72%.The cholesterol adsorption capacity of soluble dietary fiber is 28.37mg/g (pH=2.0), 34.23mg/g (pH=7.0), and sodium taurocholate adsorption capacity is 487.53mg/g.The retention ability of insoluble diedairy fiber is 9.32g/g, and holding oily power is 5.93g/g, and swellability is 9.03mL/g.
Embodiment 4
(1) pretreatment of raw material: the Pon orange peel slag obtaining after the Pon mandarin orange fresh fruit after cleaning is squeezed the juice carries out blanching processing, and heat iron temperature is 95 ℃, and blanching treatment time is 1min.Gained Pon orange peel slag after blanching, puts into the dry 36h of 60 ℃ of electric heating constant-temperature blowing drying boxes, and its water content is 5%, is crushed to 80 orders, obtains pretreated Pon orange peel ground-slag end.
(2) add aqueous dispersion: the Pon orange peel slag after step (1) is pulverized is added to aqueous dispersion, and solid-to-liquid ratio is 1g:4.5mL, stirs, and makes dispersion liquid;
(3) using microwave assisted aqueous extraction lixiviate: in step (2), dispersion liquid is positioned in microwave leaching device, and extraction temperature is 70 ℃, and solid-to-liquid ratio is 1g～20mL, and extraction time is 9min, and microwave power is 700W, extracts 2 times, obtains leaching liquor;
(4) complex enzyme carries out enzymolysis: the HCl solution that is 0.5mol/L by concentration to step (3) gained leaching liquor regulates pH value to 6.0, adds successively cellulase, pectin lyase, 1,4 beta-glucanase to carry out enzymolysis, obtains enzymolysis liquid; Cellulase addition is 3.5% of leaching liquor quality, is placed in 60 ℃ of water bath with thermostatic control shaking tables, and rotating speed is 120r/min, and enzymolysis time is 6h; The addition of pectin lyase is 0.15% of leaching liquor quality, at 40 ℃ of temperature, stirs enzymolysis 60min; The addition of 1,4 beta-glucanase is 0.10% of leaching liquor quality, at 50 ℃ of temperature, stirs enzymolysis 30min;
(5) enzyme that goes out: by gained enzymolysis liquid in step (4), at 95 ℃ of enzyme 2min that go out, be cooled to room temperature;
(6) centrifugal treating: the solution that gained in step (5) is gone out after enzyme carries out centrifugal, and rotating speed is 4200r/min, and the time is 25min, is precipitated thing and supernatant;
(7) washing is processed: will after step (6) is centrifugal, wash 3 times by gained sediment, solid-to-liquid ratio is 1g:10mL, and washing temperature is 50 ℃, and centrifugation obtains insoluble diedairy fiber sediment;
(8) concentrated, alcohol precipitation, alcohol wash are processed: will after step (6) is centrifugal, at 60 ℃, rotate evaporation and concentration to 1/13 of original volume under the vacuum of 0.09MPa for gained supernatant, and obtain concentrate.The ethanol that is 90% to the volume fraction of adding 5 times of concentrate volumes in gained concentrate carries out alcohol precipitation, and alcohol precipitation temperature is 40 ℃, and the alcohol precipitation time is 7h, and centrifugation obtains alcohol precipitation sediment.Sediment carries out alcohol wash processing, and concentration of alcohol used is 75%, and alcohol wash number of times is 2 times, and alcohol wash temperature is 35 ℃, and centrifugation obtains water-soluble dietary fiber sediment;
(9) dry processing: gained sediment in step (7) and step (8) is placed in to vacuum drying chamber dry, baking temperature is 60 ℃, and vacuum is 0.9Mpa, and be 4h drying time, obtains insoluble diedairy fiber and soluble dietary fiber;
(10) preserve: insoluble diedairy fiber and soluble dietary fiber through the dry gained of step (9) are ground into after fine powder, pack the interior sealing preservation of aluminum foil sack into or carry out product processing.
The recovery rate that obtains soluble dietary fiber and insoluble diedairy fiber under this condition is respectively 27.32% and 43.35%, and purity is respectively 92.81% and 72.93%.The cholesterol adsorption capacity of soluble dietary fiber is 30.31mg/g (pH=2.0), 36.92mg/g (pH=7.0), and sodium taurocholate adsorption capacity is 498.46mg/g.The retention ability of insoluble diedairy fiber is 11.82g/g, and holding oily power is 6.83g/g, and swellability is 10.52mL/g.
Claims (12)
1. a preparation method for Pon mandarin orange dietary fiber, is characterized in that, through the method, obtains soluble dietary fiber and insoluble diedairy fiber product, and concrete steps are as follows:
(1) pretreatment of raw material: by Pon orange peel slag hot water blanching to be processed, the Pon orange peel slag after blanching is dried, pulverizes sieving for standby;
(2) add aqueous dispersion: the Pon orange peel slag after step (1) is pulverized is added to aqueous dispersion, stir;
(3) Pon orange peel slag lixiviate: carry out lixiviate by ultrasonic wave auxiliary water extraction or using microwave assisted aqueous extraction extraction;
(4) complex enzyme carries out enzymolysis: gained leaching liquor in step (3) is regulated to pH with HCl solution, add successively cellulase, pectin lyase, 1,4 beta-glucanase to carry out enzymolysis, obtain enzymolysis liquid;
(5) enzyme that goes out: step (4) the gained enzymolysis liquid enzyme that goes out is processed;
(6) centrifugal treating: step (5) the gained solution after enzyme that goes out is clarified by centrifugal separation process, be precipitated thing and supernatant;
(7) washing is processed: will after step (6) is centrifugal, wash processing by gained sediment, centrifugation obtains insoluble diedairy fiber sediment;
(8) concentrated, alcohol precipitation, alcohol wash are processed: the centrifugal rear gained supernatant of step (6) is carried out to concentration, obtain concentrate; Gained concentrate carries out alcohol precipitation processing, and centrifugation obtains alcohol precipitation sediment, then carries out alcohol wash processing, and centrifugation obtains soluble dietary fiber sediment;
(9) vacuum drying treatment: step (7) and step (8) gained insoluble diedairy fiber sediment and soluble dietary fiber sediment are placed in to vacuum drying chamber dry, obtain insoluble diedairy fiber and soluble dietary fiber;
(10) preservation processing: insoluble diedairy fiber and soluble dietary fiber through the dry gained of step (9) are ground into after fine powder, pack the interior sealing preservation of aluminum foil sack into or carry out product processing.
2. the preparation method of a kind of Pon mandarin orange dietary fiber according to claim 1, it is characterized in that: the skin slag of the Pon orange peel slag in described step (1) for obtaining after the Pon mandarin orange fresh fruit after cleaning is squeezed the juice, its heat iron temperature is 85 ℃～100 ℃, and blanching treatment time is 1min～3min; After Pon orange peel slag draining after blanching, put into the dry 24h～48h of 50 ℃～70 ℃ electric heating constant-temperature blowing drying boxes, being dried to water content is 4%-7%, is crushed to 60～80 orders, obtains Pon orange peel ground-slag end.
3. the preparation method of a kind of Pon mandarin orange dietary fiber according to claim 1, is characterized in that: the Pon orange peel slag after pulverizing in described step (2) and the mixing ratio of water are 1g:(3mL～5mL).
4. the preparation method of a kind of Pon mandarin orange dietary fiber according to claim 1, it is characterized in that: the extraction temperature of the ultrasonic auxiliary water lixiviate in described step (3) is 40 ℃～80 ℃, solid-to-liquid ratio is 1g:(10mL～25mL), extraction time is 20min～60min, ultrasonic power is 300W～500W, dutycycle is 1:2, extracts 1～2 time.
5. the preparation method of a kind of Pon mandarin orange dietary fiber according to claim 1, it is characterized in that: the solid-to-liquid ratio 1g:(10mL～25mL of the using microwave assisted aqueous extraction lixiviate in described step (3)), extraction time is 0.5min～10min, microwave power 300W～700W, microwave temperature is 50 ℃～90 ℃, extracts 1～2 time.
6. the preparation method of a kind of Pon mandarin orange dietary fiber according to claim 1, is characterized in that, the enzyme work of described cellulase is defined as: 1min decomposes the required enzyme amount of generation 1 μ mol reduced sugar from substrate solution, and 1 Ge Meihuo unit, represents with U/mL; The enzyme of described step (4) cellulase is lived as 300U/mL～400U/mL; The enzyme work of described pectin lyase is defined as: decompose pectin per minute, and make 235nm place extinction value increase by 1.0 enzyme amount, be defined as 1 Ge Meihuo unit, with U/mL, represent; In described step (4), the enzyme of pectin lyase is lived as 400U/mL～500U/mL; The enzyme work of described 1,4 beta-glucanase is defined as: the beta glucan solution that 1g solid enzyme powder or 1mL enzyme liquid 1min are 5mg/mL from concentration, the required enzyme amount of degraded release 1 μ mol reduced sugar is defined as 1 Ge Meihuo unit, with U/mL, represents; In described step (4), the enzyme of 1,4 beta-glucanase is lived as 5000U/mL～10000U/mL.
7. the preparation method of a kind of Pon mandarin orange dietary fiber according to claim 1, it is characterized in that: the HCl solution that is 0.5mol/L by concentration in described step (4) regulates pH value to 3.5～5.5, adding cellulase addition is 1.0%～4.0% of leaching liquor quality, be placed in 50 ℃～70 ℃ water bath with thermostatic control shaking tables, rotating speed is 100r/min～120r/min, and enzymolysis time is 6h～10h; The addition of pectin lyase is 0.15%～0.30% of leaching liquor quality, at 30 ℃～60 ℃ temperature, stirs enzymolysis 30min～80min; The addition of 1,4 beta-glucanase is 0.05%～0.20% of leaching liquor quality, under 30 ℃～60 ℃ conditions, stirs enzymolysis 25min～60min.
8. the preparation method of a kind of Pon mandarin orange dietary fiber according to claim 1, is characterized in that: in described step (5), by step (4) gained enzymolysis liquid, at 95 ℃ of enzyme 2min that go out, be cooled to room temperature.
9. the preparation method of a kind of Pon mandarin orange dietary fiber according to claim 1, it is characterized in that: in described step (6), step (7) and step (8), centrifugal rotational speed is respectively 4000r/min～4200r/min, and centrifugation time is 20min～30min.
10. the preparation method of a kind of Pon mandarin orange dietary fiber according to claim 1, it is characterized in that: in described step (7), by the centrifugal rear gained sediment washing of step (6) 1～3 time, solid-to-liquid ratio is 1g:(5mL～10mL), washing temperature is 50 ℃～80 ℃, centrifugation, obtains insoluble diedairy fiber sediment.
The preparation method of 11. a kind of Pon mandarin orange dietary fibers according to claim 1, it is characterized in that: in described step (8), by the centrifugal rear gained supernatant of step (6) at 50 ℃～60 ℃, under the vacuum of 0.09MPa～0.1MPa, rotate evaporation and concentration to 1/7～1/15 of original volume, obtain concentrate; The ethanol that is 55%～95% to the volume fraction of adding 3-7 times of concentrate volume in gained concentrate carries out alcohol precipitation, and the alcohol precipitation time is 4h～12h, and alcohol precipitation temperature is 20 ℃～40 ℃, and centrifugation obtains alcohol precipitation sediment; Sediment carries out alcohol wash processing, and ethanol mass fraction used is 55%～95%, and alcohol wash number of times is 1～3 time, and alcohol wash temperature is 20 ℃～40 ℃, obtains soluble dietary fiber sediment.
The preparation method of 12. a kind of Pon mandarin orange dietary fibers according to claim 1, is characterized in that: in described step (9), vacuum drying temperature is 40 ℃～60 ℃, and vacuum is 0.6MPa～0.9MPa, and be 2h～5h drying time.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410242955.8A CN103976372B (en) | 2014-06-03 | 2014-06-03 | A kind of preparation method of Pon mandarin orange dietary fiber |
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| CN104286735A (en) * | 2014-09-20 | 2015-01-21 | 马立同 | Citrus reticulata processing technology |
| CN104306501A (en) * | 2014-10-23 | 2015-01-28 | 西安莹朴生物科技股份有限公司 | Microwave assisted extraction method for general flavones of citrus peel |
| CN104621495A (en) * | 2015-02-26 | 2015-05-20 | 吴海军 | Dragon fruit peel active dietary fiber powder and preparation method thereof |
| CN105166942A (en) * | 2015-10-09 | 2015-12-23 | 徐州工程学院 | Method for enzyme-method-assisted microwave-ultrasonic wave synergistic extraction of soluble dietary fiber from soy sauce residues |
| WO2016068738A1 (en) * | 2014-10-27 | 2016-05-06 | Beta-Bio Technology Sp. Z.O.O. | Method of isolation of beta-glucane of wheat |
| CN106213300A (en) * | 2016-07-30 | 2016-12-14 | 山东胜伟园林科技有限公司 | A kind of Salicornia Bigelovii Torr. dietary fiber meal replacement powder and preparation method thereof |
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| CN106146687B (en) * | 2016-09-28 | 2018-10-16 | 中国科学院重庆绿色智能技术研究院 | A kind of method of pectin in extraction citrus peel residue |
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| CN114431486A (en) * | 2022-03-02 | 2022-05-06 | 浙江工商大学 | Method for extracting dietary fiber from citrus peel by ultrasonic-assisted alkaline process |
| CN115336765A (en) * | 2022-08-22 | 2022-11-15 | 广东省农业科学院蚕业与农产品加工研究所 | Soluble dietary fiber, preparation method and probiotic application thereof |
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| CN104286735A (en) * | 2014-09-20 | 2015-01-21 | 马立同 | Citrus reticulata processing technology |
| CN104306501A (en) * | 2014-10-23 | 2015-01-28 | 西安莹朴生物科技股份有限公司 | Microwave assisted extraction method for general flavones of citrus peel |
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| CN104621495A (en) * | 2015-02-26 | 2015-05-20 | 吴海军 | Dragon fruit peel active dietary fiber powder and preparation method thereof |
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| CN108433113A (en) * | 2018-01-22 | 2018-08-24 | 华南理工大学 | A kind of method that ultrasonication prepares citrus fruit fibres |
| CN109645502A (en) * | 2019-01-02 | 2019-04-19 | 旬阳县太极缘生物科技有限公司 | High-quality dietary fiber, the preparation method of honey raisin tree diet fiber composition and product |
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| CN110353279A (en) * | 2019-08-22 | 2019-10-22 | 辽宁大学 | A kind of method of modifying of turnip dietary fiber |
| CN112043762A (en) * | 2020-08-17 | 2020-12-08 | 南昌大学 | Preparation method of modified and combined polyphenol of shaddock peel insoluble dietary fiber |
| CN112043762B (en) * | 2020-08-17 | 2022-02-11 | 南昌大学 | Preparation method of modified and combined polyphenol of shaddock peel insoluble dietary fiber |
| CN112314954A (en) * | 2020-11-17 | 2021-02-05 | 唐山市食品药品综合检验检测中心(唐山市畜牧水产品质量监测中心) | Preparation method of corn dietary fiber |
| CN113331430A (en) * | 2021-05-17 | 2021-09-03 | 仲恺农业工程学院 | Method for preparing soluble dietary fiber from citrus fruit peel residues |
| CN113508911A (en) * | 2021-07-21 | 2021-10-19 | 佛山职业技术学院 | Preparation method of shaddock peel dietary fiber |
| CN114431486A (en) * | 2022-03-02 | 2022-05-06 | 浙江工商大学 | Method for extracting dietary fiber from citrus peel by ultrasonic-assisted alkaline process |
| CN115336765A (en) * | 2022-08-22 | 2022-11-15 | 广东省农业科学院蚕业与农产品加工研究所 | Soluble dietary fiber, preparation method and probiotic application thereof |
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