CN102187937A - Method for producing globulin zymolytes of porcine blood - Google Patents
Method for producing globulin zymolytes of porcine blood Download PDFInfo
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Abstract
The invention relates to a method for producing globulin zymolytes of porcine blood. The method comprise the following steps of: 1, adding fresh porcine blood into solution of sodium citrate for preventing coagulation; 2, obtaining solution of hematocytes by decomposition; 3, adding water in an amount which is 0.8 to 2.5 times that of the solution of hematocytes; 4, adding animal albumen hydrolase in an amount which is 0.20 to 0.60 percent based on the weight of the solution of hematocytes for enzymolysis, regulating the pH value and stopping enzymolysis; 5, centrifuging the enzymolytic solution by using a three-leg centrifuge and a tubular solid-liquid separator respectively to obtain supernatant; 6, in the presence of active carbon, decoloring the supernatant at the temperature of between 70 and 90 DEG C for 30 to 60 minutes while stirring the solution, and standing the solution for 20 to 40 minutes; 7, centrifuging the decoloring solution by using the three-leg centrifuge and the tubular solid-liquid separator respectively to obtain the supernatant; and 8, spray-drying the supernatant to obtain the finished product. The method has the advantages of easy operation, low cost and suitability for industrialized production.
Description
Technical field:
The present invention relates to a kind of production method of animal derived feed, is to be the production method of raw material production pig hyperglobulinemia zymolyte with pig blood specifically.
Background technology:
Animal slaughtering discarded object---blood is a kind of animal protein feed raw material that has potentiality.It contains multiple nutrient and bioactivator, as protein, multiple biology enzyme.Utilize the feed goods of livestock and poultry blood production to mainly contain two kinds: the blood plasma after 1, blood anticoagulant separates is used to produce SDPP.2, the globulin powder of producing with haemocyte (or blood cell).Major protein in the blood cell---hemoglobin is a kind of mixing tetramer, is to curl the shape arrangement, and the protection of cell membrane is arranged outward, is difficult in animal body by digestibility and utilization.Feature limits such as the globulin powder fishy smell weight of making in addition,, palatability difference its application on animal produces.Therefore, improving the utilization rate of hemoglobin, is the key of proteinic powder of porcine research and utilization.
The space structure of hyperglobulinemia matter molecule is spherical, so claim hyperglobulinemia, the group of molecule and key tightly are wrapped in the inside of diameter of Spherical Volume structure, so just cause the difficulty of hydrolysis, be exactly strong acid, that the highly basic hydrolysis all is difficult to hydrolysis is complete, these reasons have all caused the high and rare situation of this kind product in the market of hyperglobulinemia zymolyte production cost.
In recent years, state, inside and outside to utilize zymolysis technique to improve the research of pig blood utilization rate more and more, the kind of hydrolase is a lot, as single enzymes such as trypsase, papain, neutral proteinase, alkali protease, food flavor enzyme and complex enzyme etc., their hydrolysis effect and financial cost have nothing in common with each other, so how to obtain the required enzyme of pig blood degraded simple, easily row, high efficiency, low expense, day by day become the key of porcine haemoglobin deep process technology.
Summary of the invention:
The objective of the invention is to overcome above-mentioned the deficiencies in the prior art, a kind of method of producing pig hyperglobulinemia zymolyte is provided, this method is carried out enzymolysis with animal proteolytic enzyme to hyperglobulinemia, with active carbon enzymolysis liquid is decoloured, have the advantages that production operation is simple, cost is low, product quality is high.
As above design, technical scheme of the present invention is: the production method of a boar hyperglobulinemia zymolyte is characterized in that: carry out according to the following step:
(1) gathers: get the anti-coagulants anti-freezing that fresh pig blood adds pig blood volume 4.5 ‰-6.0 ‰;
(2) separate: the pig blood after the anti-freezing is separated with channel separator, isolate blood plasma liquid and blood cell liquid;
(3) haemolysis: above-mentioned blood cell liquid is added 0.8-2.5 times of water dilution obtain haemocytolysis liquid, under hypotonic situation, erythrocyte membrane is broken and discharge hemoglobin;
(4) enzymolysis: the animal proteolytic enzyme that adds blood cell liquid weight 2.0 ‰-6.0 ‰ in above-mentioned haemocytolysis liquid carries out enzyme digestion reaction and obtains enzymolysis liquid;
(5) centrifugal: it is centrifugal to use tripod pendulum type batch centrifugal and tube type solid-liquid seperator that above-mentioned enzymolysis liquid is carried out respectively, obtains supernatant, removes residue;
(6) decolouring: above-mentioned supernatant is decoloured, obtain destainer;
(7) centrifugal: it is centrifugal to use tripod pendulum type batch centrifugal, tube type solid-liquid seperator that above-mentioned destainer is carried out respectively, removes active carbon, obtains supernatant;
(8) spray-drying: the centrifugal supernatant that obtains is carried out spray-drying promptly obtain final finished product.
Above-mentioned anti-coagulants employing concentration is 10% sodium citrate solution.
The enzymatic hydrolysis condition of above-mentioned enzyme digestion reaction is: keep 50-60 ℃ of constant temperature, enzymolysis 8-12h, adjust pH to 5.5-6.5.
Active carbon is adopted in above-mentioned supernatant decolouring.
The condition of above-mentioned supernatant decolouring is: under 70-90 ℃ temperature, the use active carbon leaves standstill 20-40min after stirring decolouring 30-60min.
The present invention has following advantage and good effect:
1, the present invention before the enzymolysis by after adding water and fully stirring, make blood cell cell membrane swelling, fragmentation after, hemoglobin discharges fully, greatly reduces the use amount of enzyme in the enzymolysis process, saves production cost.
2, the present invention uses this complex enzyme of animal proteolytic enzyme, and enzymolysis is settled at one go, and technology is simple, and is cheap, thorough enzymolysis, and product yield height, quality are good.
3, the present invention adopts active carbon to carry out supernatant decolouring, good decolorizing effect, simple to operate, production cost is lower.
The specific embodiment:
Embodiment 1:
Gather fresh pig blood, the concentration that adds pig blood weight 4.5 ‰ is 10% sodium citrate solution anti-freezing, separates with channel separator, isolates blood plasma liquid and blood cell liquid, gets blood cell liquid 100kg and adds the dilution of 120kg water.In haemocytolysis liquid, add the animal proteolytic enzyme of 500g, 58 ℃ of constant temperature, enzymolysis 8h, adjust pH to 6.0 enzymolysis reaction.It is centrifugal to use tripod pendulum type batch centrifugal, tube type solid-liquid seperator that enzymolysis liquid is carried out, and obtains supernatant.Supernatant uses active carbon to stir decolouring 30min under 90 ℃ temperature, leave standstill 30min after, reuse tripod pendulum type batch centrifugal, tube type solid-liquid seperator and destainer carried out centrifugal, the liquid that obtains is carried out spray-drying, obtain finished product.
After testing, adopting the method is small-molecular peptides and amino acid with the macro-molecular protein mass degradation in the blood, and amino acid content is up to 85.67%.
Embodiment 2:
Gather fresh pig blood, the concentration that adds pig blood weight 5.5 ‰ is 10% sodium citrate solution anti-freezing, separates with channel separator, isolates blood plasma liquid and blood cell liquid, and blood plasma directly carries out spray-drying, makes SDPP.Get 1 ton of blood cell liquid and add 1.5 tons of water, high-speed stirred makes blood cell haemolysis.The animal proteolytic enzyme that in haemocytolysis liquid, adds 5.5kg, 56 ℃ of constant temperature, enzymolysis 9h, adjust pH to 6.5 enzymolysis reaction.It is centrifugal to use tripod pendulum type batch centrifugal, tube type solid-liquid seperator that enzymolysis liquid is carried out, and obtains supernatant.Supernatant keeps 80 ℃, uses active carbon to stir decolouring 40min, leave standstill 25min after, reuse tripod pendulum type batch centrifugal, tube type solid-liquid seperator and destainer carried out centrifugal, the liquid that obtains is carried out spray-drying, obtain finished product 280kg.
The every index of pig hyperglobulinemia zymolyte that subordinate list: embodiment 2 obtains is as follows:
| Project | Index |
| Thick protein | 85.1％ |
| Ash content | 3.5％ |
| Moisture | 6.3％ |
| Peptide | 64％ |
Claims (5)
1. the production method of a boar hyperglobulinemia zymolyte is characterized in that: carry out according to the following step:
(1) gathers: get the anti-coagulants anti-freezing that fresh pig blood adds pig blood volume 4.5 ‰-6.0 ‰;
(2) separate: the pig blood after the anti-freezing is separated with channel separator, isolate blood plasma liquid and blood cell liquid;
(3) haemolysis: above-mentioned blood cell liquid is added 0.8-2.5 times of water dilution obtain haemocytolysis liquid, under hypotonic situation, erythrocyte membrane is broken and discharge hemoglobin;
(4) enzymolysis: the animal proteolytic enzyme that adds blood cell liquid weight 2.0 ‰-6.0 ‰ in above-mentioned haemocytolysis liquid carries out enzyme digestion reaction and obtains enzymolysis liquid;
(5) centrifugal: it is centrifugal to use tripod pendulum type batch centrifugal and tube type solid-liquid seperator that above-mentioned enzymolysis liquid is carried out respectively, obtains supernatant, removes residue;
(6) decolouring: above-mentioned supernatant is decoloured, obtain destainer;
(7) centrifugal: it is centrifugal to use tripod pendulum type batch centrifugal, tube type solid-liquid seperator that above-mentioned destainer is carried out respectively, removes active carbon, obtains supernatant;
(8) spray-drying: the centrifugal supernatant that obtains is carried out spray-drying promptly obtain final finished product.
2. the production method of a boar hyperglobulinemia zymolyte according to claim 1 is characterized in that: above-mentioned anti-coagulants employing concentration is 10% sodium citrate solution.
3. the production method of a boar hyperglobulinemia zymolyte according to claim 1 is characterized in that: the enzymatic hydrolysis condition of above-mentioned enzyme digestion reaction is: keep 50-60 ℃ of constant temperature, enzymolysis 8-12h, adjust pH to 5.5-6.5.
4. the production method of a boar hyperglobulinemia zymolyte according to claim 1 is characterized in that: active carbon is adopted in above-mentioned supernatant decolouring.
5. the production method of a boar hyperglobulinemia zymolyte according to claim 1 is characterized in that: the condition of above-mentioned supernatant decolouring is: under 70-90 ℃ temperature, the use active carbon leaves standstill 20-40min after stirring decolouring 30-60min.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103250869A (en) * | 2013-05-24 | 2013-08-21 | 东北农业大学 | Enzymolysis corpuscle powder containing polypeptide and free amino acid and preparation method thereof |
| CN103349154A (en) * | 2013-07-28 | 2013-10-16 | 杭州爱因生物科技有限公司 | Blood powder enzymolysis and decolorization method |
| CN104431413A (en) * | 2014-10-16 | 2015-03-25 | 浙江索纳克生物科技有限公司 | Method of comprehensively utilizing pig blood |
| CN105969817A (en) * | 2016-05-16 | 2016-09-28 | 金光 | Process for producing amino acid from blood of slaughtered livestock and poultry |
| CN106262901A (en) * | 2016-08-24 | 2017-01-04 | 安徽哈博药业有限公司 | A kind of Os Bovis seu Bubali protein polypeptide capsule and preparation method thereof |
| CN106343575A (en) * | 2016-08-24 | 2017-01-25 | 安徽哈博药业有限公司 | Fish roe polypeptide capsules capable of moistening lung and removing phlegm and preparation method of fish roe polypeptide capsules |
| CN110904177A (en) * | 2019-11-28 | 2020-03-24 | 湖北瑞邦生物科技有限公司 | Porcine blood cell polypeptide powder and preparation method and application thereof |
| CN112574222A (en) * | 2020-12-12 | 2021-03-30 | 江西济民可信药业有限公司 | Process for extracting high-purity heme iron from pig blood to prepare blood-benefiting crystal granule intermediate |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103250869A (en) * | 2013-05-24 | 2013-08-21 | 东北农业大学 | Enzymolysis corpuscle powder containing polypeptide and free amino acid and preparation method thereof |
| CN103349154A (en) * | 2013-07-28 | 2013-10-16 | 杭州爱因生物科技有限公司 | Blood powder enzymolysis and decolorization method |
| CN104431413A (en) * | 2014-10-16 | 2015-03-25 | 浙江索纳克生物科技有限公司 | Method of comprehensively utilizing pig blood |
| CN105969817A (en) * | 2016-05-16 | 2016-09-28 | 金光 | Process for producing amino acid from blood of slaughtered livestock and poultry |
| CN106262901A (en) * | 2016-08-24 | 2017-01-04 | 安徽哈博药业有限公司 | A kind of Os Bovis seu Bubali protein polypeptide capsule and preparation method thereof |
| CN106343575A (en) * | 2016-08-24 | 2017-01-25 | 安徽哈博药业有限公司 | Fish roe polypeptide capsules capable of moistening lung and removing phlegm and preparation method of fish roe polypeptide capsules |
| CN110904177A (en) * | 2019-11-28 | 2020-03-24 | 湖北瑞邦生物科技有限公司 | Porcine blood cell polypeptide powder and preparation method and application thereof |
| CN110904177B (en) * | 2019-11-28 | 2021-10-22 | 湖北瑞邦生物科技有限公司 | Porcine blood cell polypeptide powder and preparation method and application thereof |
| CN112574222A (en) * | 2020-12-12 | 2021-03-30 | 江西济民可信药业有限公司 | Process for extracting high-purity heme iron from pig blood to prepare blood-benefiting crystal granule intermediate |
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Application publication date: 20110921 |