CN112574222A - Process for extracting high-purity heme iron from pig blood to prepare blood-benefiting crystal granule intermediate - Google Patents
Process for extracting high-purity heme iron from pig blood to prepare blood-benefiting crystal granule intermediate Download PDFInfo
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- CN112574222A CN112574222A CN202011449969.9A CN202011449969A CN112574222A CN 112574222 A CN112574222 A CN 112574222A CN 202011449969 A CN202011449969 A CN 202011449969A CN 112574222 A CN112574222 A CN 112574222A
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- pig blood
- heme iron
- acetic acid
- blood
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- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 title claims abstract description 54
- 210000004369 blood Anatomy 0.000 title claims abstract description 41
- 239000008280 blood Substances 0.000 title claims abstract description 41
- 229910052742 iron Inorganic materials 0.000 title claims abstract description 27
- 150000003278 haem Chemical class 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title claims abstract description 20
- 239000013078 crystal Substances 0.000 title description 11
- 239000008187 granular material Substances 0.000 title description 6
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 54
- 238000005507 spraying Methods 0.000 claims abstract description 23
- 238000005119 centrifugation Methods 0.000 claims abstract description 17
- 239000000084 colloidal system Substances 0.000 claims abstract description 12
- 238000005406 washing Methods 0.000 claims abstract description 10
- 230000001112 coagulating effect Effects 0.000 claims abstract description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 42
- 229960000583 acetic acid Drugs 0.000 claims description 21
- 239000011780 sodium chloride Substances 0.000 claims description 21
- 210000000601 blood cell Anatomy 0.000 claims description 20
- 238000006243 chemical reaction Methods 0.000 claims description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- 239000012362 glacial acetic acid Substances 0.000 claims description 12
- 239000002904 solvent Substances 0.000 claims description 11
- 102000001554 Hemoglobins Human genes 0.000 claims description 9
- 108010054147 Hemoglobins Proteins 0.000 claims description 9
- 238000001291 vacuum drying Methods 0.000 claims description 9
- 239000000203 mixture Substances 0.000 claims description 7
- 239000006228 supernatant Substances 0.000 claims description 6
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 4
- 239000007787 solid Substances 0.000 claims description 4
- 239000012295 chemical reaction liquid Substances 0.000 claims description 2
- 238000000926 separation method Methods 0.000 claims description 2
- 241000282898 Sus scrofa Species 0.000 description 44
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 24
- 238000004519 manufacturing process Methods 0.000 description 10
- 239000002245 particle Substances 0.000 description 10
- 238000000227 grinding Methods 0.000 description 8
- 238000001816 cooling Methods 0.000 description 7
- 239000000463 material Substances 0.000 description 7
- 239000003918 blood extract Substances 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 238000000746 purification Methods 0.000 description 5
- 238000000605 extraction Methods 0.000 description 4
- 238000001914 filtration Methods 0.000 description 4
- 238000010438 heat treatment Methods 0.000 description 4
- 239000008213 purified water Substances 0.000 description 4
- 239000002994 raw material Substances 0.000 description 4
- 238000007670 refining Methods 0.000 description 4
- 238000012216 screening Methods 0.000 description 4
- 241000282894 Sus scrofa domesticus Species 0.000 description 3
- 238000005345 coagulation Methods 0.000 description 3
- 230000015271 coagulation Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000010298 pulverizing process Methods 0.000 description 3
- 239000001509 sodium citrate Substances 0.000 description 3
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 150000004032 porphyrins Chemical class 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 241001061264 Astragalus Species 0.000 description 1
- VTLYFUHAOXGGBS-UHFFFAOYSA-N Fe3+ Chemical compound [Fe+3] VTLYFUHAOXGGBS-UHFFFAOYSA-N 0.000 description 1
- 208000006083 Hypokinesia Diseases 0.000 description 1
- 206010024642 Listless Diseases 0.000 description 1
- 235000006545 Ziziphus mauritiana Nutrition 0.000 description 1
- 244000126002 Ziziphus vulgaris Species 0.000 description 1
- 235000008529 Ziziphus vulgaris Nutrition 0.000 description 1
- 238000000540 analysis of variance Methods 0.000 description 1
- 235000006533 astragalus Nutrition 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 208000002173 dizziness Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 229910001447 ferric ion Inorganic materials 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 208000017971 listlessness Diseases 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- -1 porphyrin compound Chemical class 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 210000004233 talus Anatomy 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
- C07D487/22—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains four or more hetero rings
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention relates to a method for extracting high-purity heme iron from pig blood, which comprises the following steps: 1) naturally coagulating fresh pig blood; 2) crushing and homogenizing by a multifunctional colloid mill; 3) centrifuging by a centrifuge; 4) preparing by an acetic acid method; 5) and (4) obtaining the high-purity heme iron after high-speed tubular centrifugation and continuous automatic spraying, washing and purifying.
Description
Technical Field
The invention relates to a medicine extraction technology, in particular to a method for extracting high-purity heme iron from pig blood, and belongs to the technical field of medicines.
Background
The heme iron, namely the porphyrin iron, is a porphyrin compound consisting of a porphyrin ring and a bivalent ferric ion, is separated and extracted from pig blood, is easy to be absorbed by a human body, and is a main intermediate for preparing blood benefiting crystal particles. The blood benefiting crystal particle is an innovative Chinese medicinal compound preparation prepared from a pig blood extract, astragalus and Chinese date, and is suitable for enriching blood and tonifying qi. Can be used for treating sallow complexion or pale complexion, dizziness, listlessness, and hypodynamia.
The existing extraction method of heme iron comprises the following steps:
filtering fresh pig blood according to the coagulation condition to remove residual blood, grinding by a colloid mill for 30 seconds, wherein the grinding particle size is 0.3cm, the material feeding temperature is 5 ℃, and the feeding speed is 1.5 t/h. Then 5 times of anhydrous acetic acid is added, 0.25 percent of sodium citrate and sodium chloride are added according to the proportion of 1:1, the mixture is refluxed and extracted for 30 minutes, the raw material feeding time is 15 minutes, the reaction temperature is 95 ℃, and the stirring speed is 30 r/min. Cooling at 20 deg.C for 24 hr, filtering with 100 mesh filter, refining with 15 times of 50% ethanol by tubular centrifugation for 20 times, vacuum drying under reduced pressure until the water content is not higher than 2.0% at 70 deg.C, and pulverizing with 120 mesh filter to obtain sanguis Sus Domestica extract.
The existing extraction method has the following defects:
the crude pig blood extract needs to be refined 20 times by tube-type centrifugal refining, 15 times of 50% ethanol is consumed each time, the production operation is very complicated, the automatic continuous level is low, the time and the labor are consumed, the ethanol consumption is huge, the production cost is high, and the pollution to the environment is large.
The invention optimizes the existing pig blood extraction process through technical innovation to prepare the high-purity heme iron and meets the industrialization requirement of high-quality blood-benefiting crystal particles.
Disclosure of Invention
The invention aims to provide a preparation method for extracting high-purity heme iron from pig blood, and the prepared heme iron has high purity and less impurities through a high-speed tubular centrifugation and continuous automatic spraying combined process technology, is beneficial to improving the quality of blood benefiting crystal particles, can save the using amount of ethanol during production, and reduces environmental pollution and production cost.
The method comprises the following steps:
1) naturally coagulating fresh pig blood;
2) crushing and homogenizing by a multifunctional colloid mill;
3) centrifuging by a centrifuge;
4) preparing by an acetic acid method;
5) and (4) obtaining the high-purity heme iron after high-speed tubular centrifugation and continuous automatic spraying, washing and purifying.
Wherein, in the step 1), fresh pig blood is naturally coagulated, and supernatant is removed to obtain hemoglobin;
in the step 2), homogenizing and crushing by using a multifunctional colloid mill to obtain homogenate, wherein the conditions for crushing and homogenizing are as follows: the time is 10-65 s;
wherein, in the step 3), a centrifuge is used for centrifuging the homogenate in the previous step, the upper layer of pig plasma is removed, and the lower layer of pig blood cells are collected, wherein the centrifugation time is 5-25 min;
wherein, in the step 4), the preparation is carried out by an acetic acid method, glacial acetic acid and sodium chloride are added into a reaction kettle, the reaction kettle is heated to 75-115 ℃ to dissolve the sodium chloride, the porcine blood cells in the previous step are slowly added, the temperature is kept at 75-115 ℃ for 10-60min, and then the mixture is cooled to room temperature, wherein the adding amount of the glacial acetic acid is 2-6 times of the weight of the porcine blood cells, and the adding amount of the sodium chloride is 0.1-0.8% of the weight of the porcine blood cells;
and in the step 5), the reaction liquid prepared by the acetic acid method flows into a high-speed tubular centrifuge through a pipeline, 10-25 times of water is added, the centrifugation is carried out, the normal operation of the high-speed tubular centrifuge is kept, the spraying is carried out through a continuous automatic spraying device, wherein the automatic washing solvent is 20-80% (v/v) ethanol water solution, the using amount of the ethanol water solution is 1-3 times of the volume of fresh pig blood, the temperature is 20-60 ℃, the centrifugation is carried out for 1-4 hours after 20-100min of the heme iron obtained by the primary centrifugal separation is sprayed, and the obtained solid heme iron is dried for 2-12 hours in a vacuum drying oven at the temperature of 40-80 ℃ to obtain the solid heme iron.
The method and technical parameters of the invention are obtained by screening, and the screening process is as follows:
in order to improve the purification effect of the pig blood extract by high-speed tubular centrifugation and continuous automatic spraying washing, the purity of heme iron is taken as an investigation index, process research is carried out aiming at a solvent, the dosage, the temperature and the centrifugation time, and process screening is carried out according to the parameters to determine the optimal pretreatment process.
TABLE 1 4-factor 3 horizontal table affecting purification effect of pig blood extract
TABLE 2 orthogonal Experimental tables for the screening of purification Effect factors
Analysis of variance of factors affecting purification effectiveness
(significant if the critical value of F is greater than 19.000.)
In summary, the following steps: the optimal purification process parameters are as follows: the refined solvent is 50% ethanol, the dosage of the refined solvent is 3 times of the volume of the fresh pig blood, the temperature of the refined solvent is 60 ℃, and the refined solvent is purified by high-speed tubular centrifugation and continuous automatic spraying.
The advantageous effects of the present invention (calculated as 200 parts by weight per batch) are further illustrated by comparative experiments as follows.
| Item | Purity of pig blood extract | The amount of ethanol | Production time per batch | Amount of waste liquid |
| Inventive example 1 | 93％ | 600Vol. | 70h | 1600Vol. |
| Inventive example 2 | 95％ | 600Vol. | 72h | 1600Vol. |
| Prior art 1 | 89％ | 3000Vol. | 140h | 4000Vol. |
| Prior art 2 | 87％ | 3000Vol. | 120h | 4000Vol. |
Wherein, in the prior art 1, the fresh pig blood is filtered to remove the residual blood according to the coagulation condition, and is ground by a colloid mill for 30 seconds, the grinding particle size is 0.3cm, the material feeding temperature is 5 ℃, and the feeding speed is 1.5 t/h. Then 5 times of anhydrous acetic acid is added, 0.25 percent of sodium citrate and sodium chloride are added according to the proportion of 1:1, the mixture is refluxed and extracted for 30 minutes, the raw material feeding time is 15 minutes, the reaction temperature is 95 ℃, and the stirring speed is 30 r/min. Cooling at 20 deg.C for 24 hr, filtering with 100 mesh filter, refining with 15 times of 50% ethanol by tubular centrifugation for 20 times, vacuum drying under reduced pressure until the water content is not higher than 2.0% at 70 deg.C, and pulverizing with 120 mesh filter to obtain sanguis Sus Domestica extract.
In the prior art 2, the fresh pig blood is filtered to remove residual blood according to the coagulation condition, and is ground by a colloid mill for 30 seconds, the grinding particle size is 0.3cm, the material feeding temperature is 5 ℃, and the feeding speed is 1.5 t/h. Then 5 times of anhydrous acetic acid is added, 0.25 percent of sodium citrate and sodium chloride are added according to the proportion of 1:1, the mixture is refluxed and extracted for 30 minutes, the raw material feeding time is 15 minutes, the reaction temperature is 95 ℃, and the stirring speed is 30 r/min. Cooling at 20 deg.C for 24 hr, filtering with 100 mesh filter, refining with 20 times of 50% ethanol by tubular centrifugation for 15 times, vacuum drying under reduced pressure until the water content is not higher than 2.0% at 70 deg.C, and pulverizing with 120 mesh filter to obtain sanguis Sus Domestica extract.
The experimental data show that the technical scheme of the invention is superior to the prior art in technical effect.
The invention provides a process for preparing a blood-benefiting crystal particle intermediate by extracting high-purity heme iron from pig blood. The inventor prepares the hemoglobin iron with high purity and few impurities by a high-speed tubular centrifugation and continuous automatic spraying combined process technology, is beneficial to improving the quality of the blood benefiting crystal particles, and provides a technical basis for the quality improvement and the efficiency increase of subsequent blood benefiting crystal particle products.
Detailed Description
The present invention will be described in further detail with reference to the following examples. This should not be understood as limiting the scope of the above-described subject matter of the present invention to the following examples. All the technologies realized based on the above contents of the present invention belong to the scope of the present invention.
The raw materials and equipment used in the embodiment of the invention are all known products.
Example 1, a process for preparing a blood-benefiting crystal granule intermediate from pig blood by extracting high-purity heme iron from pig blood comprises the steps of naturally coagulating 50kg of fresh pig blood, removing supernatant to obtain hemoglobin, grinding the hemoglobin by using a multifunctional colloid mill for 30 seconds to break and homogenize, adding a certain amount of glacial acetic acid and sodium chloride (the weight ratio of the glacial acetic acid to the pig blood cells is 3, and the sodium chloride is 0.5 percent of the weight of the pig blood cells) into a reaction kettle, heating the reaction kettle to 100 ℃, dissolving the sodium chloride, slowly adding the pig blood cells, preserving the temperature at 100 ℃ for 20min, and cooling the mixture to room temperature. Adding purified water to make the ratio of material to liquid (kg/L) 1:20, introducing into high-speed tubular centrifuge, centrifuging for 30min, starting continuous automatic spraying system (50% ethanol/water as automatic washing solvent, 50 deg.C, and spraying speed of 150L/h), keeping the high-speed tubular centrifuge running, spraying after 1h, and vacuum drying at 60 deg.C for 5 hr.
Example 2, a process for preparing a blood-benefiting crystal granule intermediate from pig blood by extracting high-purity heme iron from pig blood comprises the steps of naturally coagulating 50kg of fresh pig blood, removing supernatant to obtain hemoglobin, grinding the hemoglobin by using a multifunctional colloid mill for 30 seconds to break and homogenize, adding a certain amount of glacial acetic acid and sodium chloride (the weight ratio of the glacial acetic acid to the pig blood cells is 3, and the sodium chloride is 0.5 percent of the weight of the pig blood cells) into a reaction kettle, heating the reaction kettle to 100 ℃, dissolving the sodium chloride, slowly adding the pig blood cells, preserving the temperature at 100 ℃ for 20min, and cooling the mixture to room temperature. Adding purified water to make the ratio of material to liquid (kg/L) 1:20, introducing into high-speed tubular centrifuge, centrifuging for 30min, starting continuous automatic spraying system (50% ethanol/water as automatic washing solvent, 50 deg.C, and spraying speed of 75L/h), keeping high-speed tubular centrifuge running, spraying after 2h, and vacuum drying at 60 deg.C for 5 hr.
Example 3, a process for preparing an intermediate of Yixuejing granule from pig blood by extracting high purity heme iron from pig blood comprises the steps of naturally coagulating 50kg of fresh pig blood, removing supernatant to obtain hemoglobin, grinding with a multifunctional colloid mill for 30s to break and homogenize, adding a certain amount of glacial acetic acid and sodium chloride (the weight ratio of glacial acetic acid to pig blood cell is 3, and the sodium chloride is 0.5% of the weight of pig blood cell) into a reaction kettle, heating to 100 ℃, dissolving the sodium chloride, slowly adding the pig blood cell, keeping the temperature at 100 ℃ for 20min, and cooling to room temperature. Adding purified water to make the ratio of material to liquid (kg/L) 1:20, introducing into high-speed tubular centrifuge, centrifuging for 30min, starting continuous automatic spraying system (50% ethanol/water as automatic washing solvent, 50 deg.C, and 50L/h spraying speed), keeping the high-speed tubular centrifuge running, spraying for 3h, and vacuum drying at 60 deg.C for 5 hr.
Example 4, a process for preparing an intermediate of Yixuejing granule from pig blood by extracting high purity heme iron from pig blood comprises the steps of naturally coagulating 50kg of fresh pig blood, removing supernatant to obtain hemoglobin, grinding with a multifunctional colloid mill for 30s to break and homogenize, adding a certain amount of glacial acetic acid and sodium chloride (the weight ratio of glacial acetic acid to pig blood cell is 3, and the sodium chloride is 0.5% of the weight of pig blood cell) into a reaction kettle, heating to 100 ℃, dissolving the sodium chloride, slowly adding the pig blood cell, keeping the temperature at 100 ℃ for 20min, and cooling to room temperature. Adding purified water to make the ratio of material to liquid (kg/L) 1:20, introducing into high-speed tubular centrifuge, centrifuging for 30min, starting continuous automatic spraying system (50% ethanol/water as automatic washing solvent, 50 deg.C, and 38L/h spraying speed), keeping the high-speed tubular centrifuge running, spraying after 4h, and vacuum drying at 60 deg.C for 5 hr.
Obviously, many modifications, substitutions, and variations are possible in light of the above teachings of the invention, without departing from the basic technical spirit of the invention, as defined by the following claims.
Claims (1)
1. A method for extracting high-purity heme iron from pig blood comprises the following steps:
5) naturally coagulating fresh pig blood;
6) crushing and homogenizing by a multifunctional colloid mill;
7) centrifuging by a centrifuge;
8) preparing by an acetic acid method;
5) and (4) obtaining the high-purity heme iron after high-speed tubular centrifugation and continuous automatic spraying, washing and purifying.
Wherein, in the step 1), fresh pig blood is naturally coagulated, and supernatant is removed to obtain hemoglobin;
in the step 2), homogenizing and crushing by using a multifunctional colloid mill to obtain homogenate, wherein the conditions for crushing and homogenizing are as follows: the time is 10-65 s;
wherein, in the step 3), a centrifuge is used for centrifuging the homogenate in the previous step, the upper layer of pig plasma is removed, and the lower layer of pig blood cells are collected, wherein the centrifugation time is 5-25 min;
wherein, in the step 4), the preparation is carried out by an acetic acid method, glacial acetic acid and sodium chloride are added into a reaction kettle, the reaction kettle is heated to 75-115 ℃ to dissolve the sodium chloride, the porcine blood cells in the previous step are slowly added, the temperature is kept at 75-115 ℃ for 10-60min, and then the mixture is cooled to room temperature, wherein the adding amount of the glacial acetic acid is 2-6 times of the weight of the porcine blood cells, and the adding amount of the sodium chloride is 0.1-0.8% of the weight of the porcine blood cells;
and in the step 5), the reaction liquid prepared by the acetic acid method flows into a high-speed tubular centrifuge through a pipeline, 10-25 times of water is added, the centrifugation is carried out, the normal operation of the high-speed tubular centrifuge is kept, the spraying is carried out through a continuous automatic spraying device, wherein the automatic washing solvent is 20-80% (v/v) ethanol water solution, the using amount of the ethanol water solution is 1-3 times of the volume of fresh pig blood, the temperature is 20-60 ℃, the centrifugation is carried out for 1-4 hours after 20-100min of the heme iron obtained by the primary centrifugal separation is sprayed, and the obtained solid heme iron is dried for 2-12 hours in a vacuum drying oven at the temperature of 40-80 ℃ to obtain the solid heme iron.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998028302A1 (en) * | 1996-12-20 | 1998-07-02 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Method of obtaining haemin from slaughter blood |
| CN102187937A (en) * | 2011-03-22 | 2011-09-21 | 湖北宝迪农业科技有限公司 | Method for producing globulin zymolytes of porcine blood |
| WO2012149689A1 (en) * | 2011-05-05 | 2012-11-08 | Chen Mutao | New process for extracting chlorhematin from anymal blood |
| CN105566334A (en) * | 2015-12-18 | 2016-05-11 | 湖南益阳益威生化试剂有限公司 | Production method for preparing heme by using blood meal |
-
2020
- 2020-12-12 CN CN202011449969.9A patent/CN112574222A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998028302A1 (en) * | 1996-12-20 | 1998-07-02 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Method of obtaining haemin from slaughter blood |
| CN102187937A (en) * | 2011-03-22 | 2011-09-21 | 湖北宝迪农业科技有限公司 | Method for producing globulin zymolytes of porcine blood |
| WO2012149689A1 (en) * | 2011-05-05 | 2012-11-08 | Chen Mutao | New process for extracting chlorhematin from anymal blood |
| CN105566334A (en) * | 2015-12-18 | 2016-05-11 | 湖南益阳益威生化试剂有限公司 | Production method for preparing heme by using blood meal |
Non-Patent Citations (3)
| Title |
|---|
| 袁曦、洪清: "氯化血红素提取工艺及质量标准研究", 《中国药学杂志》 * |
| 钟耀广: "利用冰醋酸提取血红素的研究", 《食品科学》 * |
| 马永征: "血红素提取和精制方法的研究进展", 《化工文摘》 * |
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