CN1858228A - Process for preparing low molecular peptide and amino acid by biological enzymolysis of pig blood haematoglobin - Google Patents
Process for preparing low molecular peptide and amino acid by biological enzymolysis of pig blood haematoglobin Download PDFInfo
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- CN1858228A CN1858228A CN 200610034691 CN200610034691A CN1858228A CN 1858228 A CN1858228 A CN 1858228A CN 200610034691 CN200610034691 CN 200610034691 CN 200610034691 A CN200610034691 A CN 200610034691A CN 1858228 A CN1858228 A CN 1858228A
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Abstract
The present invention relates to the process of biologically enzymolyzing pig blood haematoglobin to prepare small molecular weight peptide and amino acid. The process includes adding water to pig blood haematoglobin to regulate protein content to 11.4-16.5 wt%, regulating pH value to 7.2-7.7, maintaining temperature at 50-55 deg.c adding mixture proteinase of pancereatin, Protamex and Flavourzyme or papain in the amount of 330-720 U/g to react for 12-17 hr to reach hydrolysis degree of 24-30, and centrifugally separating to eliminate insoluble part. The present invention adopts cell breaking technology, and has optimized combination of composite proteinase and optimized reaction condition to reach protein recovering rate up to 90-95%. The product has high solubility, high foamability, outstanding wet keeping performance and no bad smell and may be used widely in food production.
Description
Technical field
The present invention relates to the comprehensive utilization field of meat product processing byproduct blood protein resource, be meant that specifically the porcine hemoglobin biological enzymolysis prepares low molecular peptide and amino acid whose method.
Background technology
The animal blood that comes from the meat product processing byproduct contains the protein about 19%, suitable with protein content in the lean meat, wherein 20% is present in the blood plasma, reaching 80% in addition is present in the erythrocyte, and its amino acid Compositional balance, especially Methionin, tryptophane height are a kind of high-quality and cheap protein resource.
Mainly be that the development and use of plasma proteins have obtained certain progress to blood protein in recent years, but to being present in the oxyphorase that accounts for blood total protein content 80% in the erythrocyte, because it is difficult to be digested and assimilated, bioavailability is low and color and luster is easy to brown stain, fishy smell is difficult to dispel, even trace adds the bad change that still can cause Food Quality, still do not have terms of settlement preferably, this has just seriously restricted the development and use of a large amount of blood resources.Though have small part blood to be used for the lower product of production added value at present, as blood bean curd, blood sausage and feeding blood meal, but other most of blood directly discharges discarded, and (its chemical oxygen demand (COD) is up to 500,000mg/L) to have caused the serious wasting of resources and environmental pollution.In view of this, in today that shortage of resources and problem of environmental pollution become increasingly conspicuous, adopt modern food processing technology and biotechnology, carry out the blood protein especially intensive processing of oxyphorase resource and the research and development of series product thereof, significant to the bottleneck problem that solves present blood resource intensive processing.
Begin that foreign scholar Tybor, Autio are just arranged the seventies in eighties of last century, Jana í na, employing organic solvent method, carboxymethyl cellulose (CMC) methods such as Kuppevelt, Hayakawa extract globin from oxyphorase.Though the former protein recovery can reach 81.4%, dissolvent residual is arranged and be not suitable in foodstuffs industry, using; The latter's nutrition and functional performance are all relatively good, can only reach 60% but protein recovery is the highest, and the cost height, as yet not large-scale production.Buckley K., Jeng-Huh Yang and Wismer-Pedersen, people such as J. adopt the peroxide oxidation method to slough the color of oxyphorase respectively, have obtained certain effect, but the residual hydrogen peroxide of these class methods can have influence on the hygienic safety of food.Also there was scholar (Houlier, Synowiecki, Ockerman) to adopt the Alcalase enzyme hydrolysis method to extract globin in the oxyphorase afterwards, protein recovery can reach 70-80%, but enzymolysis product has heavier bitter taste, has influenced the popularization and the use of this method.
Summary of the invention
The objective of the invention is to the bottleneck problem can not mass-producing in foodstuffs industry is produced used at porcine hemoglobin; provide a kind of porcine hemoglobin biological enzymolysis to prepare low molecular peptide and amino acid whose method; make protein recovery bring up to 90-95%; non-secondary pollution of the present invention; almost reach zero release; and product do not have bitter taste, has very high solvability and whipability, has outstanding performance of keeping humidity concurrently.
Porcine hemoglobin biological enzymolysis of the present invention prepares low molecular peptide and amino acid whose method, comprises the steps:
(1) porcine hemoglobin adds water to adjust its protein content is 11.4-16.5% weight, regulator solution pH value is to 7.2-7.7, temperature remains on 50-55 ℃, the mixing protease that adds pancreatin and Protamex, Flavourzyme or papoid, enzyme is 330-720U/g with the protein concentration ratio, reaction times is 12-17h, and degree of hydrolysis is controlled at 24-30%;
(2) hydrolysate that step (1) is obtained is removed undissolved precipitation part by centrifugation.
In the step (1), described proteolytic enzyme is and is extensive use of and safe food grade enzyme in foodstuffs industry, and the concrete ratio of its mixing protease (U/U) is as follows:
Pancreatin: Protamex=1-2: 9;
Pancreatin: Flavourzyme=1-2: 4;
Pancreatin: papoid=1-2: 8.
In the step (2), the hydrolysate that step (1) can be obtained is centrifugal 10-20min under 20 ℃, 3000g-4000g condition, removes undissolved precipitation part.
It is 25-30% that the product of step (2) is concentrated into concentration, and its protein recovery can reach 90-95%, and product does not have bitter taste.Concentrate the back spraying drying, obtain powdery porcine hemoglobin zymolyte.
In step (1), can the pH value of solution value be adjusted to 7.2-7.7 with 2M NaOH.
Step (1) hydrolysis reaction can heat 5-10min and sterilize after finishing under 95-100 ℃ of condition, enzymolysis product is cooled to 25 ℃.
Described porcine hemoglobin can adopt prior art to obtain, the present invention adopts following method to prepare: the qualified live pig of quarantining is taken a blood sample through hollow knife, add 0.33% Trisodium Citrate and mix anti-freezing, pass through 3000-4000g, 5-10 ℃ of centrifugal 10-20min then, obtain the lower sediment erythrocyte, the water that erythrocyte adds 1.0-1.5 times of volume is that the broken born of the same parents of 1000-1500W supersound process 2-3min obtain porcine hemoglobin through 50-60Mpa homogeneous or ultrasonic power again.
The present invention compared with prior art, have following advantage: different proteolytic enzyme and hydrolysising condition all have material impact to the local flavor of the degraded of oxyphorase, product and functional performance and protein recovery, the present invention adopts the preparation of Controlled-enzymatic Hydrolysis technology degraded oxyphorase to meet low molecular peptide and the amino acid that food industry applications requires by proteolytic enzyme best of breed high-pressure homogeneous or that the broken erythrocyte of supersound process goes out in conjunction with screening and optimizing.This method reaction conditions gentleness, non-secondary pollution, protein recovery is significantly increased than other method, reaches as high as 95%, has improved 15-20% than the best level of present bibliographical information.Product does not have bad local flavor, has very high solvability and whipability, has outstanding performance of keeping humidity concurrently.
Embodiment
Embodiment 1
The first step: the qualified live pig of quarantining is taken a blood sample through hollow knife, add 0.33% Trisodium Citrate and mix anti-freezing, it is centrifugal 20 minutes to pass through 3000g, 5 ℃ then, obtain the lower sediment erythrocyte, the water that erythrocyte adds 1.0 times of volumes obtains porcine hemoglobin through the broken born of the same parents of 50Mpa homogeneous again, gets porcine hemoglobin 500g.
Second step: the 500g porcine hemoglobin adds water, and to adjust its protein content be 14.5%, mix the back and the pH value of solution value is adjusted to 7.5 with 2MNaOH, heating and when maintaining the temperature at 55 ℃, add pancreatin and Protamex, ratio is pancreatin: Protamex=1.8: 9 (U/U), [enzyme/albumen] concentration=710U/g, enzymolysis 13h under the temperature constant state, degree of hydrolysis is 25%, and reaction finishes the back and heat 5min under 100 ℃ of conditions, and enzymolysis product is cooled to 25 ℃.
The 3rd step: will cool off posthydrolysis product centrifugal 20min under 20 ℃, 3000g condition, and remove undissolved precipitation part, clear liquid is concentrated, and sample concentration is 25-30%.
The 4th step: the sample spraying drying after concentrating obtains product.Through check, its main component is that molecular weight is low molecular peptide and the amino acid below 10,000, and content is 93.7% with proteinometer, and protein recovery is 94.5%.
Embodiment 2
The first step: the qualified live pig of quarantining is taken a blood sample through hollow knife, add 0.33% Trisodium Citrate and mix anti-freezing, it is centrifugal 10 minutes to pass through 4000g, 10 ℃ then, obtain the lower sediment erythrocyte, the water that erythrocyte adds 1.4 times of volumes obtains porcine hemoglobin through the broken born of the same parents of 60Mpa homogeneous again, gets porcine hemoglobin 500g.
Second step: the 500g porcine hemoglobin adds water, and to adjust its protein content be 11.6%, mix the back and the pH value of solution value is adjusted to 7.2 with 2MNaOH, heating and when maintaining the temperature at 51 ℃, add pancreatin and Protamex, ratio is pancreatin: Protamex=1.1: 9 (U/U), [enzyme/albumen] concentration=670U/g, enzymolysis 16h under the temperature constant state, degree of hydrolysis is 28%, and reaction finishes the back and heat 5min under 100 ℃ of conditions, and enzymolysis product is cooled to 25 ℃.
The 3rd step: will cool off posthydrolysis product centrifugal 10min under 20 ℃, 4000g condition, and remove undissolved precipitation part, clear liquid is concentrated, and sample concentration is 26-30%.
The 4th step: the sample spraying drying after concentrating obtains product.Through check, its main component is that molecular weight is low molecular peptide and the amino acid below 10,000, and content is 92.9% with proteinometer, and protein recovery is 93.7%.
Embodiment 3
The first step: the qualified live pig of quarantining is taken a blood sample through hollow knife, add 0.33% Trisodium Citrate and mix anti-freezing, it is centrifugal 15 minutes to pass through 3500g, 5 ℃ then, obtain the lower sediment erythrocyte, the water that erythrocyte adds 1.2 times of volumes is that the broken born of the same parents of the ultrasonic 3min of 1300W obtain porcine hemoglobin through ultrasonic power again, gets porcine hemoglobin 5kg.
Second step: the 5kg porcine hemoglobin adds water, and to adjust its protein content be 12.5%, with 2M NaOH the pH value of solution value is adjusted to 7.5 then, heating and when maintaining the temperature at 53 ℃, add pancreatin and papoid, ratio is a pancreatin: papoid=1.9: 8 (U/U), [enzyme/albumen] concentration=650U/g, enzymolysis 12h under the temperature constant state, degree of hydrolysis is 24%, and reaction finishes the back and heat 10min under 100 ℃ of conditions, and enzymolysis product is cooled to 25 ℃.
The 3rd step: will cool off posthydrolysis product centrifugal 15min under 20 ℃, 3500g condition, and remove undissolved precipitation part, with supernatant concentration (ultimate density is 26%).
The 4th step: the sample spraying drying after concentrating obtains product.Through check, its main component is that molecular weight is low molecular peptide and the amino acid below 10,000, and content is 93.1% with proteinometer, and protein recovery is 92.9%.
Embodiment 4
The first step: the qualified live pig of quarantining is taken a blood sample through hollow knife, add 0.33% Trisodium Citrate and mix anti-freezing, it is centrifugal 12 minutes to pass through 3800g, 7 ℃ then, obtain the lower sediment erythrocyte, the water that erythrocyte adds 1.5 times of volumes is that the broken born of the same parents of the ultrasonic 3min of 1000W obtain porcine hemoglobin through ultrasonic power again, gets porcine hemoglobin 5kg.
Second step: the 5kg porcine hemoglobin adds water, and to adjust its protein content be 11.5%, with 2MNaOH the pH value of solution value is adjusted to 7.5 then, heating and when maintaining the temperature at 51 ℃, add pancreatin and papoid, ratio is a pancreatin: papoid=1.2: 8 (U/U), [enzyme/albumen] concentration=610U/g, enzymolysis 16h under the temperature constant state, degree of hydrolysis is 29%, and reaction finishes the back and heat 10min under 95 ℃ of conditions, and enzymolysis product is cooled to 25 ℃.
The 3rd step: will cool off posthydrolysis product centrifugal 15min under 20 ℃, 3500g condition, and remove undissolved precipitation part, with supernatant concentration (ultimate density is 29%).
The 4th step: the sample spraying drying after concentrating obtains product.Through check, its main component is that molecular weight is low molecular peptide and the amino acid below 10,000, and content is 91.4% with proteinometer, and protein recovery is 93.0%.
Embodiment 5
The first step: the qualified live pig of quarantining is taken a blood sample through hollow knife, add 0.33% Trisodium Citrate and mix anti-freezing, it is centrifugal 10 minutes to pass through 4000g, 10 ℃ then, obtain the lower sediment erythrocyte, the water that erythrocyte adds 1.5 times of volumes obtains porcine hemoglobin through the broken born of the same parents of 60Mpa homogeneous again, gets porcine hemoglobin 30kg.
Second step: the 30kg porcine hemoglobin adds water, and to adjust its protein content be 11.4%, mix the back and the pH value of solution value is adjusted to 7.6 with 2MNaOH, heating and when maintaining the temperature at 50 ℃, add pancreatin and Flavourzyme, ratio is pancreatin: Flavourzyme=1.8: 4 (U/U), [enzyme/albumen] concentration=380U/g, enzymolysis 13h under the constant temperature, degree of hydrolysis is 25%, and reaction finishes the back and heat 8min under 100 ℃ of conditions, and enzymolysis product is cooled to 25 ℃.
The 3rd step: will cool off centrifugal 10min under 20 ℃ of posthydrolysis products, the 4000g condition, and remove undissolved precipitation part, with supernatant concentration to 27%.
The 4th step: the sample spraying drying after concentrating obtains product.Through check, its main component is that molecular weight is low molecular peptide and the amino acid below 10,000, and content is 94.0% with proteinometer, and protein recovery is 90.6%.
Embodiment 6
The first step: the qualified live pig of quarantining is taken a blood sample through hollow knife, add 0.33% Trisodium Citrate and mix anti-freezing, it is centrifugal 20 minutes to pass through 3000g, 5 ℃ then, obtain the lower sediment erythrocyte, the water that erythrocyte adds 1.0 times of volumes is that the broken born of the same parents of the ultrasonic 2.5min of 1300W obtain porcine hemoglobin through ultrasonic power again, gets porcine hemoglobin 30kg.
Second step: the 30kg porcine hemoglobin adds water, and to adjust its protein content be 16.4%, mix the back and the pH value of solution value is adjusted to 7.2 with 2MNaOH, heating and when maintaining the temperature at 54 ℃, add pancreatin and Flavourzyme, ratio is pancreatin: Flavourzyme=1.0: 4 (U/U), [enzyme/albumen] concentration=330U/g, enzymolysis 17h under the temperature constant state, degree of hydrolysis is 30%, and reaction finishes the back and heat 10min under 100 ℃ of conditions, and enzymolysis product is cooled to 25 ℃.
The 3rd step: will cool off centrifugal 20min under 20 ℃ of posthydrolysis products, the 3000g condition, and remove undissolved precipitation part, with supernatant concentration to 26%.
The 4th step: the sample spraying drying after concentrating obtains product.Through check, its main component is that molecular weight is low molecular peptide and the amino acid below 10,000, and content is 92.6% with proteinometer, and protein recovery is 91.1%.
Claims (6)
1, a kind of porcine hemoglobin biological enzymolysis prepares low molecular peptide and amino acid whose method, it is characterized in that comprising the steps:
(1) porcine hemoglobin adds water to adjust its protein content is 11.4-16.5% weight, regulator solution pH value is to 7.2-7.7, temperature remains on 50-55 ℃, the mixing protease that adds pancreatin and Protamex, Flavourzyme or papoid, enzyme is 330-720U/g with the protein concentration ratio, reaction times is 12-17h, and degree of hydrolysis is controlled at 24-30%;
(2) hydrolysate that step (1) is obtained is removed undissolved precipitation part by centrifugation.
2, method according to claim 1 is characterized in that in the step (1), described mixing protease usage ratio (U/U) is as follows:
Pancreatin: Protamex=1-2: 9;
Pancreatin: Flavourzyme=1-2: 4;
Pancreatin: papoid=1-2: 8.
3, method according to claim 1 and 2 is characterized in that in the step (1), with 2MNaOH regulator solution pH.
4, method according to claim 3, it is characterized in that hydrolysis reaction finishes in the step (1) after, under 95-100 ℃ of condition, heat 5-10min, enzymolysis product is cooled to 25 ℃.
5, method according to claim 4 is characterized in that in the step (2), and the hydrolysate that step (1) is obtained is centrifugal 10-20min under 20 ℃, 3000g-4000g condition, removes undissolved precipitation part.
6, method according to claim 5, it is characterized in that described porcine hemoglobin adopts following method to prepare: the qualified live pig of quarantining is taken a blood sample through hollow knife, add 0.33% Trisodium Citrate and mix anti-freezing, pass through 3000-4000g, 5-10 ℃ of centrifugal 10-20min then, obtain the lower sediment erythrocyte, the water that erythrocyte adds 1.0-1.5 times of volume is that the broken born of the same parents of 1000-1500W supersound process 2-3min obtain porcine hemoglobin through 50-60Mpa homogeneous or ultrasonic power again.
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Cited By (7)
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| CN101250572B (en) * | 2007-10-29 | 2011-06-08 | 中国农业大学 | Method for extracting pig blood antibiotic peptide |
| CN102187937A (en) * | 2011-03-22 | 2011-09-21 | 湖北宝迪农业科技有限公司 | Method for producing globulin zymolytes of porcine blood |
| CN101550178B (en) * | 2008-03-31 | 2012-01-11 | 四川省中医药科学院 | Blood polypeptide and preparation method and application thereof |
| CN103484516A (en) * | 2012-06-12 | 2014-01-01 | 武汉恩彼生物科技有限公司 | Preparation method of high quality blood cell protein peptide by using two-step enzymolysis method |
| CN104431413A (en) * | 2014-10-16 | 2015-03-25 | 浙江索纳克生物科技有限公司 | Method of comprehensively utilizing pig blood |
| CN108753895A (en) * | 2018-07-02 | 2018-11-06 | 华中农业大学 | A kind of small molecule heme peptide and preparation method thereof |
| WO2023074991A1 (en) * | 2021-10-26 | 2023-05-04 | 주식회사 아미노랩 | Method for production of bioactive substance using blood and application thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1029373C (en) * | 1991-04-24 | 1995-07-26 | 顾佩兰 | Technological method of amino-acid extracted by graded hydrolysis of pig blood |
| CN1266103A (en) * | 1999-03-08 | 2000-09-13 | 楼伟民 | Method for making biological protein |
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- 2006-03-28 CN CNB2006100346912A patent/CN100439510C/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101250572B (en) * | 2007-10-29 | 2011-06-08 | 中国农业大学 | Method for extracting pig blood antibiotic peptide |
| CN101550178B (en) * | 2008-03-31 | 2012-01-11 | 四川省中医药科学院 | Blood polypeptide and preparation method and application thereof |
| CN102187937A (en) * | 2011-03-22 | 2011-09-21 | 湖北宝迪农业科技有限公司 | Method for producing globulin zymolytes of porcine blood |
| CN103484516A (en) * | 2012-06-12 | 2014-01-01 | 武汉恩彼生物科技有限公司 | Preparation method of high quality blood cell protein peptide by using two-step enzymolysis method |
| CN103484516B (en) * | 2012-06-12 | 2016-01-13 | 武汉恩彼生物科技有限公司 | A kind of preparation method of two step enzymolysis process production high-quality hyperglobulinemia peptides |
| CN104431413A (en) * | 2014-10-16 | 2015-03-25 | 浙江索纳克生物科技有限公司 | Method of comprehensively utilizing pig blood |
| CN104431413B (en) * | 2014-10-16 | 2017-09-22 | 浙江索纳克生物科技有限公司 | A kind of method for comprehensively utilizing pig blood |
| CN108753895A (en) * | 2018-07-02 | 2018-11-06 | 华中农业大学 | A kind of small molecule heme peptide and preparation method thereof |
| WO2023074991A1 (en) * | 2021-10-26 | 2023-05-04 | 주식회사 아미노랩 | Method for production of bioactive substance using blood and application thereof |
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