CN107011457A - A kind of method that extraction prepares SNSP and small molecule nutrient molecule in waste water from sweet potato - Google Patents

A kind of method that extraction prepares SNSP and small molecule nutrient molecule in waste water from sweet potato Download PDF

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CN107011457A
CN107011457A CN201710369519.0A CN201710369519A CN107011457A CN 107011457 A CN107011457 A CN 107011457A CN 201710369519 A CN201710369519 A CN 201710369519A CN 107011457 A CN107011457 A CN 107011457A
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sweet potato
snsp
small molecule
waste water
solution
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CN107011457B (en
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胡晓君
胡顺波
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Linyi University
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    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08BPOLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/006Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07CACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C67/00Preparation of carboxylic acid esters
    • C07C67/48Separation; Purification; Stabilisation; Use of additives
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07DHETEROCYCLIC COMPOUNDS
    • C07D311/00Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
    • C07D311/02Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
    • C07D311/04Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
    • C07D311/22Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4
    • C07D311/26Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3
    • C07D311/28Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3 with aromatic rings attached in position 2 only
    • C07D311/30Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3 with aromatic rings attached in position 2 only not hydrogenated in the hetero ring, e.g. flavones
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07DHETEROCYCLIC COMPOUNDS
    • C07D311/00Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
    • C07D311/02Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
    • C07D311/04Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
    • C07D311/22Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4
    • C07D311/26Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 4 with aromatic rings attached in position 2 or 3
    • C07D311/40Separation, e.g. from natural material; Purification
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07JSTEROIDS
    • C07J1/00Normal steroids containing carbon, hydrogen, halogen or oxygen, not substituted in position 17 beta by a carbon atom, e.g. estrane, androstane
    • C07J1/0003Androstane derivatives
    • C07J1/0011Androstane derivatives substituted in position 17 by a keto group
    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08BPOLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0003General processes for their isolation or fractionation, e.g. purification or extraction from biomass

Abstract

The invention discloses the method that extraction in a kind of waste water from sweet potato prepares SNSP, small molecule nutrient molecule.Specifically, using various ways such as filtering, elution, biological enzymolysis, successfully preparing the SNSP and flavones, dehydroepiandros-sterone, chlorogenic acid small molecule nutritional ingredient product of higher degree.The present invention prepares sweet potato SNSP and small molecule nutriment using extraction in the sweet potato waste water that starch from sweet potato is dumped is produced so that this discarded object of sweet potato waste water obtains Efficient Conversion utilization, reduces environmental pollution, improves economic benefit;The low, efficiency high of accompanying method power consumption of the present invention, recovery rate are high, environment-friendly, industrial production prospect and wide market.

Description

Extraction prepares SNSP and small molecule nutrient molecule in a kind of waste water from sweet potato Method
Technical field
Field is utilized the invention belongs to Sewage treatment, and in particular to one kind extraction from sweet potato waste water prepares SNSP And the method for small molecule nutrient molecule.
Background technology
Sweet potato is one of big main food of China four, is one of the most healthy food that the World Health Organization announces.From red The sweet potato deep processing mode that starch from sweet potato is presently the most common is prepared in potato, the technological process of its traditional mode of production is as follows:Raw material Selection-washing-, which crush-grinds to filter-convert slurry-slash cylinder and sit cylinder-slash is starched-, plays powder-drying.Wherein, it is upper during cylinder is skimmed Layer swill not only causes ambient water quality to pollute, is also the waste to active ingredient in sweet potato directly as sweet potato discharge of wastewater.According to Statistics, the enterprise of only 1000 tons starch from sweet potato of an annual output, the sweet potato waste water dumped every year is just more than 20,000 tons.
Due in sweet potato waste water containing the multiple nutritional components such as starch, albumen, polysaccharide, in the prior art it has been reported that from red Extracted in potato waste water and prepare sweet potato mucus protein, however, according to the literature, also containing abundant carbon hydrate in sweet potato waste water A variety of small molecule nutrient molecules such as thing and flavones, dehydroepiandros-sterone, chlorogenic acid, wherein, sweet potato SNSP is a kind of light brown The pulverulent solids of color, slightly sugar and sweet potato fragrance, the mixing that it is made up of reduced sugar, pentose, uronic acid or glycoprotein is more Sugar, molecular weight is in 30~40kD or so, with higher physiologically active, such as remove free radical, anti-oxidant, hypoglycemic, reducing blood lipid, Improve the effect such as immunity.And the small molecule such as flavones, dehydroepiandros-sterone, chlorogenic acid nutrient molecule also reported with it is anti-oxidant, The multiple beneficials such as anti-inflammation and sterilization, anticancer are acted on.Therefore offer one kind is needed badly to extract SNSP and flavones from sweet potato waste water, go The method of the small molecule nutritional ingredient such as hydrogen meter androsterone, chlorogenic acid, so that problem of environmental pollution can be solved, while can also improve red Sweet potato starch added value of product, but so far, not yet there is pertinent literature report.
The content of the invention
For above-mentioned the deficiencies in the prior art, the present invention provide one kind extraction from sweet potato waste water prepare SNSP and The method of small molecule nutrient molecule.The low, efficiency high of this method power consumption, recovery rate are high, environment-friendly, industrial production prospect and city Field has a extensive future.
Specifically, technical scheme is as follows:
A kind of method that extraction prepares SNSP and small molecule nutrient molecule in waste water from sweet potato, including following step Suddenly:
(1) sweet potato waste water centrifugal is separated, obtains sweet potato supernatant fluid;Starch is precipitated as to return in starch from sweet potato preparation technology It is continuing with;
(2) the sweet potato supernatant fluid obtained in step (1) is used into the membrane filtration that aperture is 0.45nm;Wherein, filter Mainly contain and mainly included in the small molecule such as flavones, dehydroepiandros-sterone, chlorogenic acid nutritional ingredient and inorganic ions, Liquid Residue in liquid Protein, SNSP;
(3) pH is adjusted to be alkalescence filtered solution, loading macroreticular resin is enriched with, blade diameter length ratio 1:3~1:5, applied sample amount is 10BV~50BV, 10~20BV/h of flow velocity, use volumetric concentration to be eluted for 60% ethanol solution, the consumption of ethanol solution is 1BV~3BV, obtains the ethanol eluate containing the small molecule nutritional ingredient such as flavones, dehydroepiandros-sterone, chlorogenic acid;Ethanol is washed De- liquid removes ethanol, suction filtration it is dry the small molecule nutritional ingredient crude product such as flavones, dehydroepiandros-sterone, chlorogenic acid;
(4) Liquid Residue adjusts pH to be alkalescence, and 1.0~4.0% (Tot Prot) compound proteases enzymolysis is added into Liquid Residue Processing;
(5) after enzymolysis processing, into Liquid Residue, (volume ratio is 5 to addition chloroform-n-butanol:1) extracted, so that egg White precipitation, obtains SNSP solution;
(6) suction filtration after decolorization is carried out to SNSP solution and is drying to obtain SNSP crude product.
It is preferred that, centrifugal treating condition is in the step (1):Rotating speed is 1000~3000rpm, and the time is 1~5min;
Filter sizes are 0.45nm in the step (2);Experiment proves that, can be at utmost by small point using above-mentioned aperture Sub- nutritional ingredient and proteinpolysaccharide separation;
It is preferred that, pH is adjusted to 8~9 in the step (3), macroreticular resin for it is nonpolar or in polar macroporous resin, enter One step is preferably X-5 macroreticular resins;
It is preferred that, pH is adjusted to 8~9 in the step (4), the compound protease be Alcalase alkali proteases, The mixture of papain and trypsase, it is further preferred that the Alcalase alkali proteases, papain and The enzyme activity of trypsase is 50000U/g, and the mass ratio of three is 1-3:3:0.5 (is preferably 1:3:0.5);The ferment treatment Actual conditions is:Treatment temperature is 40~50 DEG C, and rotating speed is 100~120rpm, and processing time is 2~4h;
It is preferred that, decolorization concretely comprises the following steps in the step (6):Taken off successively using hydrogen peroxide and ethanol Color so that hydrogen peroxide and ethanol mass fraction in SNSP solution are respectively 10% and 20%.
The flavones that is prepared the invention also discloses said extracted preparation method, dehydroepiandros-sterone, chlorogenic acid small molecule Nutritional ingredient product and sweet potato SNSP product.
The principle of the invention:Because SNSP, flavones, dehydroepiandros-sterone, chlorogenic acid small molecule etc. are sought in sweet potato waste water The content formed point is relatively low, and wherein flavones, dehydroepiandros-sterone, chlorogenic acid equimolecular quantity is smaller (< 1kD), with protein, many Sugared equimolecular quantity significant difference, thus directly from filter membrane by the small molecule such as flavones, dehydroepiandros-sterone, chlorogenic acid nutriment with Polysaccharide, protein are separated, while separation, inspissation are played again to protein and SNSP, so as to save manpower And materials and equipment resources;Meanwhile, although containing flavones, dehydroepiandros-sterone, chlorogenic acid in filtered solution, simultaneously also containing abundant nothing Machine ion, therefore from specific macroreticular resin and by parameter adjustment, so as to successfully three is enriched with, separation and concentration effect Good, while macroreticular resin is reusable, further reduction prepares cost;
Due to also containing more rich protein in Liquid Residue, meanwhile, part SNSP in sweet potato SNSP It is combined together with protein by the way that glucosides is strong, therefore to obtain purer SNSP, need to be by protein and non-starch Polysaccharide is separated, and commonly uses Sevage method (chloroform-n-butanols 5:1) protein in Polysaccharide removing, this method mild condition, but It is extremely inefficient, it is necessary to which protein as much as possible could be removed by repeatedly (having document report to repeat 20~30 times), together When, extract solution also causes polysaccharide sample to be lost repeatedly, so that cause yield to reduce, therefore its industrial application value of the method is relatively low, Applicant has been surprisingly found that, in Sevage methods before digested in advance using specific compound protease, can be effectively by egg in sweet potato White matter is digested, and then only needs to maximize using 1-2 i.e. achievable albumen removal efficiency of Sevage methods, simultaneously because using Sevage methods remove number of times and greatly reduced, and also greatly reduce polysaccharide loss amount, farthest remain sweet potato non-starch many Sugar.
Simultaneously as the substance that show color in sweet potato waste water is complicated, phenol sulfonate and reduced sugar and amino acid are mainly included The brown polymer of the high relative molecular mass generated is reacted, inventor is final to determine to use hydrogen peroxide by constantly groping Oxidative decoloration and ethanol alcohol precipitation decolouring mode, can effectively remove the substance that show color in sweet potato waste water, while to avoid single make The destruction of the SNSP esterification degree caused with high concentration hydrogen peroxide oxidative decoloration, so as to further influence non-shallow lake after purification The indexs such as powder active polysaccharide, color and luster.
Beneficial effects of the present invention:The present invention is non-using extraction preparation sweet potato in the sweet potato waste water that starch from sweet potato is dumped is produced Starch-polysaccharides and small molecule nutriment so that this discarded object of sweet potato waste water obtains Efficient Conversion utilization, reduces environment dirty Dye, improves economic benefit;The low, efficiency high of accompanying method power consumption of the present invention, recovery rate are high, environment-friendly, before industrialized production Scape and wide market.
Embodiment
It is noted that described further below is all exemplary, it is intended to provide further instruction to the application.Unless another Indicate, all technologies used herein and scientific terminology are with usual with the application person of an ordinary skill in the technical field The identical meanings of understanding.
It should be noted that term used herein above is merely to describe embodiment, and be not intended to restricted root According to the illustrative embodiments of the application.As used herein, unless the context clearly indicates otherwise, otherwise singulative Be also intended to include plural form, additionally, it should be understood that, when in this manual use term "comprising" or " comprising " When, it indicates existing characteristics, step, operation, device, component or combinations thereof.
As background technology is introduced, due to containing the carbohydrate enriched and flavones in sweet potato waste water, removing hydrogen meter A variety of small molecule nutrient molecules such as androsterone, chlorogenic acid, and do not have pertinent literature report in the prior art.
In view of this, extracted in a kind of typical embodiment of the invention there is provided one kind from sweet potato waste water prepare it is non- The method of starch-polysaccharides and small molecule nutrient molecule, comprises the following steps:
(1) sweet potato waste water centrifugal is separated, obtains sweet potato supernatant fluid;Starch is precipitated as to return in starch from sweet potato preparation technology It is continuing with;
(2) the sweet potato supernatant fluid obtained in step (1) is used into the membrane filtration that aperture is 0.45nm;Wherein, filter Mainly contain and mainly included in the small molecule such as flavones, dehydroepiandros-sterone, chlorogenic acid nutritional ingredient and inorganic ions, Liquid Residue in liquid Protein, SNSP;
(3) pH is adjusted to be alkalescence filtered solution, loading macroreticular resin is enriched with, blade diameter length ratio 1:3~1:5, applied sample amount is 10BV~50BV, 10~20BV/h of flow velocity, use volumetric concentration to be eluted for 60% ethanol solution, the consumption of ethanol solution is 1BV~3BV, obtains the ethanol eluate containing the small molecule nutritional ingredient such as flavones, dehydroepiandros-sterone, chlorogenic acid;Ethanol is washed De- liquid removes ethanol, suction filtration it is dry the small molecule nutritional ingredient crude product such as flavones, dehydroepiandros-sterone, chlorogenic acid;
(4) Liquid Residue adjusts pH to be alkalescence, and 1.0~4.0% (Tot Prot) compound proteases enzymolysis is added into Liquid Residue Processing;
(5) after enzymolysis processing, into Liquid Residue, (volume ratio is 5 to addition chloroform-n-butanol:1) extracted, so that egg White precipitation, obtains SNSP solution;
(6) suction filtration after decolorization is carried out to SNSP solution and is drying to obtain SNSP crude product.
In the another exemplary embodiment of the present invention, centrifugal treating condition is in step (1):Rotating speed be 1000~ 3000rpm, the time is 1~5min;
Filter sizes are 0.45nm in step (2);Experiment proves that, at utmost small molecule can be sought using above-mentioned aperture Form and point separated with proteinpolysaccharide;
In the another exemplary embodiment of the present invention, pH is adjusted to 8~9 in step (3), macroreticular resin for it is nonpolar or in Polar macroporous resin, more preferably X-5 macroreticular resins;
In the another exemplary embodiment of the present invention, pH is adjusted to 8~9 in step (4), and the compound protease is The mixture of Alcalase alkali proteases, papain and trypsase, it is further preferred that the Alcalase is alkaline The enzyme activity of protease, papain and trypsase is 50000U/g, and the mass ratio of three is 1-3:3:0.5 (is preferably 1:3:0.5);The ferment treatment actual conditions is:Treatment temperature is 40~50 DEG C, and rotating speed is 100~120rpm, and processing time is 2~4h;
In the another exemplary embodiment of the present invention, decolorization concretely comprises the following steps in step (6):Dioxygen is used successively Water and ethanol are decolourized so that hydrogen peroxide and ethanol mass fraction in SNSP solution are respectively 10% and 20%.
Flavones, the dehydrogenation prepared in the another exemplary embodiment of the present invention there is provided said extracted preparation method Epiandrosterone, chlorogenic acid small molecule nutritional ingredient product and sweet potato SNSP product.
The present invention is explained in order to further there is provided following examples and comparative.
Embodiment 1
A kind of method that extraction prepares SNSP and small molecule nutrient molecule in waste water from sweet potato, including following step Suddenly:
(1) sweet potato waste water is separated into (rotating speed is 2000rpm, and the time is 3min) through centrifuge, obtains sweet potato supernatant Body;It is precipitated as being continuing with starch return starch from sweet potato preparation technology;
(2) the sweet potato supernatant fluid obtained in step (1) is used into the membrane filtration that aperture is 0.45nm;Wherein, filter Mainly contain and mainly included in the small molecule such as flavones, dehydroepiandros-sterone, chlorogenic acid nutritional ingredient and inorganic ions, Liquid Residue in liquid Protein, SNSP;
(3) pH is adjusted to be 8 filtered solution, loading X-5 macroreticular resins are enriched with, blade diameter length ratio 1:5, applied sample amount is 20BV, stream Fast 10BV/h, uses volumetric concentration to be eluted for 60% ethanol solution, and the consumption of ethanol solution is 2BV, obtains containing flavones, goes The ethanol eluate of the small molecule nutritional ingredient such as hydrogen meter androsterone, chlorogenic acid;Ethanol eluate is removed into ethanol, suction filtration is yellowly dry The small molecule nutritional ingredient crude product such as ketone, dehydroepiandros-sterone, chlorogenic acid;
(4) Liquid Residue adjusts pH to be 9, and 2.0% (Tot Prot) compound protease enzymolysis processing is added into Liquid Residue;It is described Compound protease is the mixture of Alcalase alkali proteases, papain and trypsase, the Alcalase alkalescence The enzyme activity of protease, papain and trypsase is 50000U/g, and the mass ratio of three is 1:3:0.5;At the enzyme Managing actual conditions is:Treatment temperature is 45 DEG C, and rotating speed is 100rpm, and processing time is 3h;
(5) after enzymolysis processing, into Liquid Residue, (volume ratio is 5 to addition chloroform-n-butanol:1) extraction 2 times is carried out, so that Make albumen precipitation, obtain SNSP solution;
(6) suction filtration after decolorization is carried out to SNSP solution and is drying to obtain SNSP crude product, decolorization Concretely comprise the following steps:Decolourized successively using hydrogen peroxide and ethanol so that hydrogen peroxide and ethanol in SNSP solution Mass fraction is respectively 10% and 20%.
Embodiment 2
A kind of method that extraction prepares SNSP and small molecule nutrient molecule in waste water from sweet potato, including following step Suddenly:
(1) sweet potato waste water is separated into (rotating speed is 1000rpm, and the time is 5min) through centrifuge, obtains sweet potato supernatant Body;It is precipitated as being continuing with starch return starch from sweet potato preparation technology;
(2) the sweet potato supernatant fluid obtained in step (1) is used into the membrane filtration that aperture is 0.45nm;Wherein, filter Mainly contain and mainly included in the small molecule such as flavones, dehydroepiandros-sterone, chlorogenic acid nutritional ingredient and inorganic ions, Liquid Residue in liquid Protein, SNSP;
(3) pH is adjusted to be 8 filtered solution, loading X-5 macroreticular resins are enriched with, blade diameter length ratio 1:3, applied sample amount is 30BV, stream Fast 20BV/h, uses volumetric concentration to be eluted for 60% ethanol solution, and the consumption of ethanol solution is 1BV, obtains containing flavones, goes The ethanol eluate of the small molecule nutritional ingredient such as hydrogen meter androsterone, chlorogenic acid;Ethanol eluate is removed into ethanol, suction filtration is yellowly dry The small molecule nutritional ingredient crude product such as ketone, dehydroepiandros-sterone, chlorogenic acid;
(4) Liquid Residue adjusts pH to be 9, and 3.0% (Tot Prot) compound protease enzymolysis processing is added into Liquid Residue;It is described Compound protease is the mixture of Alcalase alkali proteases, papain and trypsase, the Alcalase alkalescence The enzyme activity of protease, papain and trypsase is 50000U/g, and the mass ratio of three is 2:3:0.5;At the enzyme Managing actual conditions is:Treatment temperature is 40 DEG C, and rotating speed is 100rpm, and processing time is 3h;
(5) after enzymolysis processing, into Liquid Residue, (volume ratio is 5 to addition chloroform-n-butanol:1) extraction 2 times is carried out, so that Make albumen precipitation, obtain SNSP solution;
(6) suction filtration after decolorization is carried out to SNSP solution and is drying to obtain SNSP crude product, decolorization Concretely comprise the following steps:Decolourized successively using hydrogen peroxide and ethanol so that hydrogen peroxide and ethanol in SNSP solution Mass fraction is respectively 10% and 20%.
Embodiment 3
A kind of method that extraction prepares SNSP and small molecule nutrient molecule in waste water from sweet potato, including following step Suddenly:
(1) sweet potato waste water is separated into (rotating speed is 2000rpm, and the time is 5min) through centrifuge, obtains sweet potato supernatant Body;It is precipitated as being continuing with starch return starch from sweet potato preparation technology;
(2) the sweet potato supernatant fluid obtained in step (1) is used into the membrane filtration that aperture is 0.45nm;Wherein, filter Mainly contain and mainly included in the small molecule such as flavones, dehydroepiandros-sterone, chlorogenic acid nutritional ingredient and inorganic ions, Liquid Residue in liquid Protein, SNSP;
(3) pH is adjusted to be 8 filtered solution, loading X-5 macroreticular resins are enriched with, blade diameter length ratio 1:5, applied sample amount is 10BV, stream Fast 20BV/h, uses volumetric concentration to be eluted for 60% ethanol solution, and the consumption of ethanol solution is 3BV, obtains containing flavones, goes The ethanol eluate of the small molecule nutritional ingredient such as hydrogen meter androsterone, chlorogenic acid;Ethanol eluate is removed into ethanol, suction filtration is yellowly dry The small molecule nutritional ingredient crude product such as ketone, dehydroepiandros-sterone, chlorogenic acid;
(4) Liquid Residue adjusts pH to be 8, and 4.0% (Tot Prot) compound protease enzymolysis processing is added into Liquid Residue;It is described Compound protease is the mixture of Alcalase alkali proteases, papain and trypsase, the Alcalase alkalescence The enzyme activity of protease, papain and trypsase is 50000U/g, and the mass ratio of three is 1:3:0.5;At the enzyme Managing actual conditions is:Treatment temperature is 45 DEG C, and rotating speed is 120rpm, and processing time is 1h;
(5) after enzymolysis processing, into Liquid Residue, (volume ratio is 5 to addition chloroform-n-butanol:1) extraction 3 times is carried out, so that Make albumen precipitation, obtain SNSP solution;
(6) suction filtration after decolorization is carried out to SNSP solution and is drying to obtain SNSP crude product, decolorization Concretely comprise the following steps:Decolourized successively using hydrogen peroxide and ethanol so that hydrogen peroxide and ethanol in SNSP solution Mass fraction is respectively 10% and 20%.
Comparative example 1
Method be the same as Example 1, difference is to replace X-5 using macroreticular resin HPD-100.
Comparative example 2
Method be the same as Example 1, difference is to replace X-5 using macroreticular resin D-101.
Comparative example 3
Method be the same as Example 1, difference is to replace X-5 using macroreticular resin HPD-826.
Determine remaining bavin flavones in filtered solution after absorption, dehydroepiandros-sterone, chlorogenic acid small molecule nutritional ingredient it is total Concentration, calculates embodiment 1 and comparative example 1-3 adsorption rate, the results are shown in Table 1.
Flavones, dehydroepiandros-sterone, the total concentration of chlorogenic acid small molecule nutritional ingredient in eluent are determined, embodiment 1 is calculated With comparative example 1-3 desorption efficiency, 1 the results are shown in Table.
Table 1
Meanwhile, calculate tetra- kinds of macroreticular resins of embodiment 1 and comparative example 1-3 to flavones, dehydroepiandros-sterone, chlorogenic acid absorption Amount, the results are shown in Table 2.
Table 2
X-5 models macroporous resin adsorption and desorption effect are much better than other polymeric adsorbents it can be seen from Tables 1 and 2.
Comparative example 4
Method be the same as Example 1, difference is that loading flow velocity is adjusted to 5BV/h.
Comparative example 5
Method be the same as Example 1, difference is that loading flow velocity is adjusted to 20BV/h.
Comparative example 6
Method be the same as Example 1, difference is that loading flow velocity is adjusted to 30BV/h.
Comparative example 7
Method be the same as Example 1, difference is that loading flow velocity is adjusted to 40BV/h.
After completion of the sample, macroreticular resin in embodiment 1, comparative example 4-6 is determined small to flavones, dehydroepiandros-sterone, chlorogenic acid Total adsorption rate of molecular nutrition composition, the results are shown in Table 3;
Table 3
As a result show, when loading flow velocity is relatively low, macroreticular resin is higher to the adsorption rate of flavones, dehydroepiandros-sterone, chlorogenic acid, With the increase of flow velocity, macroreticular resin is reduced to the adsorption rate of flavones, dehydroepiandros-sterone, chlorogenic acid.But loading flow velocity is also unsuitable Relatively low, flow velocity is too low to reduce production efficiency, increase production cost.Therefore, adsorption effect and production efficiency are considered, with reality The loading flow velocity 10BV/h that example 1 used is applied to be advisable.
Comparative example 8
Method be the same as Example 1, difference is the blade diameter length ratio of macroreticular resin being adjusted to 1:2.
Comparative example 9
Method be the same as Example 1, difference is the blade diameter length ratio of macroreticular resin being adjusted to 1:6.
Comparative example 10
Method be the same as Example 1, difference is the blade diameter length ratio of macroreticular resin being adjusted to 1:9.
Embodiment 1, comparative example 8-10 macroreticular resins are determined to flavones, dehydroepiandros-sterone, chlorogenic acid small molecule nutritional ingredient Total adsorption rate and desorption efficiency, the results are shown in Table 4;
Table 4
As a result show, when resin blade diameter length ratio is 1:When 9, adsorbance and desorption efficiency are best.But it is actual with reference to industrial production, with The resin blade diameter length ratio 1 that embodiment 1 is used:5 be optimum condition.
Comparative example 11
Method be the same as Example 1, difference is without using compound protease.
Comparative example 12
Method be the same as Example 1, difference is that compound protease is papain and trypsase, and the two mass ratio is 1:1.
Comparative example 13
Method be the same as Example 1, difference is that compound protease is Alcalase alkali proteases and trypsase, the two matter Amount is than being 1:1.
Comparative example 14
Method be the same as Example 1, difference is compound protease for Alcalase alkali proteases and papain, the two Mass ratio is 1:1.
Comparative example 15
Method be the same as Example 1, difference is that hydrogen peroxide, which is used only, carries out oxidative decoloration.
The sweet potato SNSP index of correlation that embodiment 1 and comparative example 11-15 are obtained is measured, specific determine refers to It is designated as, using UV scanning spectrophotometer, is scanned, as a result shows in 200~300nm, only embodiment 1 is prepared Polysaccharide solution at 260nm and 280nm without absworption peak, show embodiment 1 without the impurity component such as nucleic acid and protein;
Hydroxyl radical free radical ability is removed as index using the sweet potato SNSP prepared, to embodiment 1 and comparative example The SNSP that 11-15 is obtained is measured, as a result as shown in table 5.
Table 5
The preferred embodiment of the application is the foregoing is only, the application is not limited to, for the skill of this area For art personnel, the application can have various modifications and variations.It is all within spirit herein and principle, made any repair Change, equivalent substitution, improvement etc., should be included within the protection domain of the application.

Claims (10)

1. a kind of method that extraction prepares SNSP and small molecule nutrient molecule in waste water from sweet potato, it is characterised in that bag Include following steps:
(1) sweet potato waste water centrifugal is separated, obtains sweet potato supernatant fluid;
(2) the sweet potato supernatant fluid obtained in step (1) is used into the membrane filtration that aperture is 0.45nm;
(3) pH is adjusted to be alkalescence the filtered solution in step (2), loading macroreticular resin is enriched with, blade diameter length ratio 1:3~1:5, loading Measure as 10BV~50BV, 10~20BV/h of flow velocity, use volumetric concentration to be eluted for 60% ethanol solution, the consumption of ethanol solution For 1BV~3BV;Dry to eluent suction filtration flavones, dehydroepiandros-sterone, chlorogenic acid small molecule nutritional ingredient crude product.
(4) adjust pH to be alkalescence the Liquid Residue in step (2), 1.0~4.0% (Tot Prots) are added into Liquid Residue and are combined egg White enzyme enzymolysis processing;
(5) after the completion of enzymolysis processing, into Liquid Residue, (volume ratio is 5 to addition chloroform-n-butanol:1) extracted, so that egg White precipitation, obtains SNSP solution;
(6) suction filtration after decolorization is carried out to SNSP solution and is drying to obtain SNSP crude product.
2. the method as described in claim 1, it is characterised in that centrifugal treating condition is in the step (1):Rotating speed is 1000 ~3000rpm, the time is 1~5min.
3. the method as described in claim 1, it is characterised in that pH is adjusted to 8~9 in the step (3), and macroreticular resin is non- Polarity or in polar macroporous resin.
4. method as claimed in claim 3, it is characterised in that the macroreticular resin is X-5 macroreticular resins.
5. the method as described in claim 1, it is characterised in that pH is adjusted to 8~9 in the step (4).
6. the method as described in claim 1, it is characterised in that compound protease is Alcalase alkalescence in the step (4) The mixture of protease, papain and trypsase.
7. method as claimed in claim 6, it is characterised in that ferment treatment actual conditions is:Treatment temperature is 40~50 DEG C, is turned Speed is 100~120rpm, and processing time is 2~4h.
8. method as claimed in claim 6, it is characterised in that the Alcalase alkali proteases, papain and pancreas The enzyme activity of protease is 50000U/g, and the mass ratio of three is 1-3:3:0.5, preferably 1:3:0.5.
9. method as claimed in claim 1, it is characterised in that decolorization concretely comprises the following steps in the step (6):Adopt successively Decolourized with hydrogen peroxide and ethanol so that hydrogen peroxide and ethanol mass fraction in SNSP solution are respectively 10% He 20%.
10. the flavones that any one of claim 1-9 methods described is prepared, dehydroepiandros-sterone, the nutrition of chlorogenic acid small molecule into Divide product and sweet potato SNSP product.
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