CN101139402A - Method for extracting polysaccharide from jujube - Google Patents
Method for extracting polysaccharide from jujube Download PDFInfo
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- CN101139402A CN101139402A CNA200710018899XA CN200710018899A CN101139402A CN 101139402 A CN101139402 A CN 101139402A CN A200710018899X A CNA200710018899X A CN A200710018899XA CN 200710018899 A CN200710018899 A CN 200710018899A CN 101139402 A CN101139402 A CN 101139402A
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Abstract
The present invention discloses a method tobextract polysaccharide from the jujube. The extraction process is of the following steps: (A) jujube is mixed with water and is extracted for 1 to three times; then the jujube experiences the centrifugal separation and vacuum low-pressure concentration; (B) clarifying agent is added into the jujube mass which is statically placed for centrifugal separation after stirring; (C) the separated sypernatant is added with alcohol, which is more than 70 percent in volume; the alcohol in the sypernatant is deposited overnight, and then the sypernatant experiences centrifugal separation; (D) the solid obtained is dried to get jujube polysaccharides. The jujube polysaccharides obtained through the present invention is more than 40 percent of the original jujube. The whole extraction and separation process involves no toxic and hazardous organic solvents. The present invention is safe and non-toxic. The operation is simple and the consumed time is short. A full green extraction can be achieved through the present invention.
Description
Technical field
The present invention relates to a kind of method of from date, extracting polysaccharide, belong to the natural product extracting technique field.
Background technology
Date is the dry mature fruit of Rhamnaceae plant jujube, is tcm clinical practice tonifying Qi medicine and food commonly used, also is the industrial raw material of preparation date essence.Date is in the history of utilizing of existing more than 3,000 year of China, and distribution range is extremely wide, main product in Hebei, Shandong, Shanxi, Henan, Shan Xiwu economizes, five province's ultimate productions account for more than 90% of national jujube ultimate production.Date it distinguish the flavor of sweet good to eat, nutritious, its main component is carbohydrate, vitamin c, protein and crude fat, not only edible but also can be used as medicine, have enriching yin and nourishing kidney, improve the health, multiple medicinal function such as vessel softening, Appetizing spleen-tonifying, the laudatory title of " kings of all kinds of fruits " is arranged.
The method of extracting effective components from plant---vegetable polysaccharides has many, wherein most widely used is traditional method---water extraction and alcohol precipitation method, it can remove impurity such as protein in the extracting solution, phlegmatic temperament, fat-soluble pigment effectively, but find in the practical application that there are some defectives in it, for example target extract polysaccharide more easily loses in the alcohol precipitation process, and consumption alcohol amount is big, the cost height, the alcohol yield is big, and technical process is long etc.In addition, the extracting method of polysaccharide is also had soxhlet extraction, ultrasonic extraction, Enzymatic Extraction etc., but all there is complex process in these methods in varying degrees, the operational cycle is long, shortcomings such as production cost height.
In recent years, a kind of new medicine water extract process for purification---adsorption clarification is widely used in the field of refinement of Chinese traditional medicine water extract with characteristics such as it is simple to operate, consuming time less, cost is low, safety non-toxic, no remnants.Therefore, a large amount of natural polymer flocculate and clarify agent grow up gradually, as gelatin, honey, egg white, chitosan, ZTC1+1 finings, 101 fruit juice clarifiers or the like.Wherein, chitosan is simple with its preparation, and cost is low, has good biology solubility and biological degradability, and safety non-toxic is to be widely used in one of traditional Chinese medicine liquid and the important finings of preparation purified.In jujube polysaccharide water extract, chitosan-NH
2Protonated formation-NH
3 +After, form positively charged polyelectrolyte, can on a part, adsorb a plurality of contaminant particles (as protein, pigment material etc.), and form the very fast sedimentation of flco, thereby demonstrate excellent purification.
At present, the great majority report mainly is with traditional extraction and separation method extraction separation polysaccharide from date, perhaps extracts polysaccharide from other plant (as the Radix Astragali, matrimony vine), and does not see that the relevant report of from date extracting polysaccharide identical with this law arranged.
Summary of the invention
Main purpose of the present invention is to provide the method for extracting polysaccharide from date of a kind of mild condition, technology simple possible, safety non-toxic.
Another purpose of the present invention is to provide the polyoses extract that is obtained by aforesaid method.
The concrete extracting method of jujube polysaccharide of the present invention is as follows:
A kind of method of extracting polysaccharide from date comprises the steps:
(A) with jujube powder extracting in water 1-3 time, centrifugation, concentrating under reduced pressure;
(B) add finings, leave standstill centrifugation after stirring;
(C) add ethanol in the separating obtained supernatant liquor, the alcoholic acid volumetric concentration is reached more than 70%, centrifugation behind the alcohol precipitation overnight;
(D) the gained solid drying is promptly got jujube polysaccharide.
In extraction step, be to add water in 1: 10～1: 20 by jujube powder and water weight ratio, refluxing extraction at twice under 50～90 ℃ of conditions, centrifugation; United extraction liquid, being evaporated to jujube powder and extracting solution mass volume ratio is 1: 5～1: 10g/ml; The finings that adds leaves standstill centrifugation after stirring, and described finings is a chitosan-acetic acid solution, and chitosan-acetic acid solution is 1 gram chitosan to be dissolved in 100ml 1% acetic acid obtain.
Advantage of the present invention and positively effect: the present invention extracts polysaccharide and has adopted novel adsorption clarification technology from date, has simple to operately, and consuming time few, cost is low, safety non-toxic, advantages such as noresidue.Traditional water extraction and alcohol precipitation method gained polysaccharide content is about 30%, compares with traditional water extraction and alcohol precipitation method, and this method gained polysaccharide content increases, and reaches more than 40%.
Description of drawings
Fig. 1 is a process flow diagram of the present invention.
Embodiment
Embodiment 1:
As shown in Figure 1, take by weighing through stoning and be dried to the date 1Kg of constant weight, add distilled water 15L, in three refluxing extraction of 80 ℃ of branches, three times reflux extracting time is 2h, centrifugation; Merging the extracting solution of gained, is 1 by date and quality volume ratio: the 8g/ml concentrating under reduced pressure, in concentrated solution, add 1% chitosan-acetic acid solution 1L, and leave standstill certain hour after stirring, centrifugation; To add dehydrated alcohol in the supernatant liquor, make the alcoholic acid volumetric concentration reach 70%, centrifugation behind the alcohol precipitation overnight; The gained solid drying to constant weight, is promptly got the jujube polysaccharide product.The gained polysaccharide yield is 4.71%, content 46.5%.
Embodiment 2:
Take by weighing through stoning and be dried to the date 20g of constant weight, add distilled water 200ml, in 85 ℃ of refluxing extraction at twice, twice reflux extracting time is 2h, centrifugation; Merging the extracting solution of gained, is 1 by date and quality volume ratio: the 5g/ml concentrating under reduced pressure, in concentrated solution, add 1% chitosan-acetic acid solution 20ml, and leave standstill certain hour after stirring, centrifugation; To add dehydrated alcohol in the supernatant liquor, make the alcoholic acid volumetric concentration reach 70%, centrifugation behind the alcohol precipitation overnight; The gained solid drying to constant weight, is promptly got the jujube polysaccharide product.The gained polysaccharide yield is 4.82%, content 46.7%.
Claims (6)
1. a method of extracting polysaccharide from date comprises the steps:
(A) with date extracting in water 1-3 time, centrifugation, concentrating under reduced pressure;
(B) add finings, leave standstill centrifugation after stirring;
(C) add ethanol in the separating obtained supernatant liquor, the alcoholic acid volumetric concentration reached more than 70%,
Centrifugation behind the alcohol precipitation overnight;
(D) the gained solid drying is promptly got jujube polysaccharide.
2. according to the described method of extracting polysaccharide from date of claim 1, it is characterized in that: by date and water weight ratio is to add water in 1: 10～1: 20, refluxing extraction at twice under 50～90 ℃ of conditions, centrifugation.
3. according to the described method of extracting polysaccharide from date of claim 2, it is characterized in that: united extraction liquid, being evaporated to date and extracting solution mass volume ratio is 1: 5～1: 10g/ml.
4. according to the described method of extracting polysaccharide from date of claim 1, it is characterized in that: the finings of adding is a chitosan-acetic acid solution.
5. the method for extracting polysaccharide in the described date of claim 1 is made up of following step:
(A) be to add water in 1: 10～1: 20 by date and water weight ratio, in 85 ℃ of refluxing extraction at twice, twice reflux extracting time is 2h, centrifugation;
(B) extracting solution of merging gained, being evaporated to jujube powder and extracting solution mass volume ratio is 1: 5～1: 10g/ml;
(C) add 1% chitosan-acetic acid solution, leave standstill centrifugation after stirring;
(D) in separating obtained supernatant liquor, add dehydrated alcohol, make the alcoholic acid volumetric concentration reach 70%, centrifugation behind the alcohol precipitation overnight;
(E) with the gained solid drying to constant weight, promptly get the jujube polysaccharide product.
6. jujube polysaccharide extract of taking one of claim 1 to 5 any means to make.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA200710018899XA CN101139402A (en) | 2007-10-18 | 2007-10-18 | Method for extracting polysaccharide from jujube |
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| CNA200710018899XA CN101139402A (en) | 2007-10-18 | 2007-10-18 | Method for extracting polysaccharide from jujube |
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| CN101139402A true CN101139402A (en) | 2008-03-12 |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101358219B (en) * | 2008-07-14 | 2011-06-08 | 天津农学院 | Simultaneous sequential chemical extraction of jujube flavones and jujube polysaccharide by fermentation method |
| CN101288468B (en) * | 2008-05-23 | 2011-07-20 | 山西省农业科学院农产品综合利用研究所 | Method of sequential extraction of biological activity component from jujube |
| CN102302118A (en) * | 2011-08-18 | 2012-01-04 | 天津大学 | Astragalus and Chinese date instant granules and preparation method thereof |
| CN104277136A (en) * | 2014-09-24 | 2015-01-14 | 山东省千佛山医院 | Yellow river beach date polysaccharides, and extraction and refining method and application thereof |
| CN105399852A (en) * | 2015-12-14 | 2016-03-16 | 陈燕妮 | Technology for producing radix astragali polysaccharide by utilization of alkaline process extraction technology |
| CN105906738A (en) * | 2016-06-29 | 2016-08-31 | 欣格瑞（山东）环境科技有限公司 | Method for extracting and separating polysaccharides from Celosia cristata |
| CN106380525A (en) * | 2016-08-29 | 2017-02-08 | 安徽兆晨农业科技发展有限公司 | Chinese date sugar extraction method |
| CN109350687A (en) * | 2018-09-20 | 2019-02-19 | 江苏省中医药研究院 | A kind of composition of strengthen immunity and its preparation method and application |
| CN113456584A (en) * | 2021-07-12 | 2021-10-01 | 国药集团新疆制药有限公司 | Preparation method of six-ingredient saffron oral liquid |
-
2007
- 2007-10-18 CN CNA200710018899XA patent/CN101139402A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101288468B (en) * | 2008-05-23 | 2011-07-20 | 山西省农业科学院农产品综合利用研究所 | Method of sequential extraction of biological activity component from jujube |
| CN101358219B (en) * | 2008-07-14 | 2011-06-08 | 天津农学院 | Simultaneous sequential chemical extraction of jujube flavones and jujube polysaccharide by fermentation method |
| CN102302118A (en) * | 2011-08-18 | 2012-01-04 | 天津大学 | Astragalus and Chinese date instant granules and preparation method thereof |
| CN104277136A (en) * | 2014-09-24 | 2015-01-14 | 山东省千佛山医院 | Yellow river beach date polysaccharides, and extraction and refining method and application thereof |
| CN105399852A (en) * | 2015-12-14 | 2016-03-16 | 陈燕妮 | Technology for producing radix astragali polysaccharide by utilization of alkaline process extraction technology |
| CN105906738A (en) * | 2016-06-29 | 2016-08-31 | 欣格瑞（山东）环境科技有限公司 | Method for extracting and separating polysaccharides from Celosia cristata |
| CN105906738B (en) * | 2016-06-29 | 2019-03-22 | 欣格瑞（山东）环境科技有限公司 | The method of separating polyose is extracted in a kind of cockscomb |
| CN106380525A (en) * | 2016-08-29 | 2017-02-08 | 安徽兆晨农业科技发展有限公司 | Chinese date sugar extraction method |
| CN109350687A (en) * | 2018-09-20 | 2019-02-19 | 江苏省中医药研究院 | A kind of composition of strengthen immunity and its preparation method and application |
| CN113456584A (en) * | 2021-07-12 | 2021-10-01 | 国药集团新疆制药有限公司 | Preparation method of six-ingredient saffron oral liquid |
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