CN105399852A - Technology for producing radix astragali polysaccharide by utilization of alkaline process extraction technology - Google Patents
Technology for producing radix astragali polysaccharide by utilization of alkaline process extraction technology Download PDFInfo
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- CN105399852A CN105399852A CN201510921893.8A CN201510921893A CN105399852A CN 105399852 A CN105399852 A CN 105399852A CN 201510921893 A CN201510921893 A CN 201510921893A CN 105399852 A CN105399852 A CN 105399852A
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
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Abstract
The invention relates to the radix astragali medicine extraction technology field, and concretely provides a technology for producing radix astragali polysaccharide by utilization of an alkaline process extraction technology. The method comprises steps: dried radix astragali roots are taken, cleaned, dried, crushed, added with an alkaline hydrolysis liquid, and soaked for 2-3h at a constant temperature, extraction is repeated for three times, the extracting solutions are merged and the mixture is allowed to stand for cooling, a natural plant extracting solution clarificant KBT-ZTC is added, the crude extracting solution is condensed through rotary evaporation after standing and an extract crude product is obtained; ethanol is added in the extract crude product, the extract is stirred and dissolved, the mixture is allowed to stand for precipitation, centrifugation is carried out, the supernatant is removed and sediments are obtained; ethanol is added again, stirring and centrifugation are carried out and a pure product radix astragali polysaccharide is prepared. Based on a normal water extraction alcohol precipitation method, the plant traditional Chinese medicine clarification technology is introduced to achieve extraction and purification of high-quality radix astragali polysaccharide. The extraction yield of active ingredients in radix astragali polysaccharide is raised, and the radix astragali polysaccharide extraction rate of the improved method is more than 10.6%, the highest being 13.5%.
Description
Technical field
The invention belongs to Radix Astragali extract drugs technical field, particularly relate to the technique utilizing alkalinity extraction technology to produce astragalus polysaccharides.
Background technology
The Radix Astragali is fabaceous, and taste is sweet, slightly warm in nature, returns lung, the spleen channel, there is tonifying Qi and lifting yang, beneficial taste shown admittedly, inducing diuresis to remove edema, promote the production of body fluid nourish blood, expelling pus and toxin by strengthening QI, the effect such as expelling pus and promoting granulation.Astragalus polysaccharides (APS) is the polysaccharide extracted from Chinese medicine astragalus, primarily of compositions such as fructose, rhamnosyl, pectinose, hexuronic acid, glucose, galacturonic acid and glucuronic acids.Because of its have strengthen function of immune system, antitumor, protect the liver, the effect such as hypoglycemic, antiviral, resisting oxidation and delaying senility.In veterinary clinic, astragalus polysaccharides has obvious effect at immunomodulatory, antiviral, antibiosis, is widely used, can be used to prevent grice diarrhoea, Porcine circovirus desease, porcine influenza, blue otopathy etc. with immunostimulant and immunological adjuvant.In addition, astragalus polysaccharides can also promote growth of animals or poultry, improves breeding performonce fo animals, shortens the rabbit culture-cycle etc.In recent years along with the expansion of astragalus polysaccharides clinical application, many scholars have carried out large quantity research to the extraction and separation process of astragalus polysaccharides, but still there is general lower, the problem such as extraction cost is high, leaching process is loaded down with trivial details, power consumption is high of astragalus polysaccharides extraction yield.
Astragalus polysaccharides water soluble, is insoluble to alcohol.Current water extraction is the traditional method extracting herbal polysaccharide, and water extraction method is simple, easy to operation, but energy consumption is comparatively large, and extracting method is unstable, extracts the purity of polysaccharide obtained not high, how containing impurity such as protein, nucleic acid, starch.The present invention is in order to the astragalus polysaccharides purity solving above-mentioned water extraction and extract is not high, extract the large problem of energy consumption, according to the physicochemical property of effective constituent most of in astragalus polysaccharides and in order to cost-saving, introduce finings dedoping step, impurity in effective removing pharmaceutical cpd, reduce the energy consumption of leaching process because needing pyroprocess to produce, and extractive technique technical process is tended towards stability, method is reproducible.
Summary of the invention
The object of the invention is to overcome existing medical glycyrrhiza and extract the shortcomings such as astragalus polysaccharides purity, extraction yield are low, a kind of technique utilizing water extraction technology to produce astragalus polysaccharides is provided.
Actualizing technology scheme of the present invention is, a kind of technique utilizing alkalinity extraction technology to produce astragalus polysaccharides, and be raw material with the Radix Astragali, it is characterized in that, the method step comprises:
1) crude extract acquisition: dry astragalus root raw material 1kg, after cleaning, drying, pulverizing, add 2 ~ 3 times of alkali solution liquids, under 40 ~ 50 DEG C of conditions, constant temperature soaked after 2 ~ 3 hours, repeated extraction 3 times, and merging alkali solution liquid liquid leaves standstill and cools, and obtains crude extract;
2) concentration and evaporation: add natural botanical extraction liquid finings KBT-ZTC in the crude extract that step 1) obtains, add-on is 5 ~ 10g/L, and after leaving standstill 20 ~ 30min, rotary evaporation concentrates crude extract, obtains medicinal extract crude product;
3) removal of impurities is dissolved: in step 2) add ethanol in the medicinal extract crude product that obtains, stir medicinal extract and dissolve, staticly settle, centrifugal segregation supernatant liquor is precipitated thing, throw out adds ethanol again to carry out leaving standstill after stirring, and centrifugal collecting precipitate, obtains sterling astragalus polysaccharides.
As further qualifications of the present invention, described cleaning is for using clean water 2 ~ 3 times; Described pulverizing is that astragalus membranaceus powder is broken to 50 ~ 100 orders.
As further qualifications of the present invention, it is the medicinal extract of 1.0 ~ 1.50 that crude extract is evaporated to water proportion d by described rotary evaporation simmer down to.
As further qualifications of the present invention, in described step 3), the add-on of ethanol is 2 ~ 5 times of volumes of medicinal extract.
As further qualifications of the present invention, described alkali solution liquid is the mixing solutions of sodium carbonate, sodium bisulfite, calcium chloride, and sodium carbonate, sodium bisulfite, the calcium chloride content in leaching solution is respectively 15 ~ 30%, 15 ~ 30%, 10 ~ 40%.
As further qualifications of the present invention, described natural botanical extraction liquid finings KBT-ZTC is the finings that You Ke Lier bio tech ltd produces.
The present invention achieves following substantial advance and outstanding effect:
Start with medicinal Astragalis, high-quality astragalus polysaccharides is extracted through series of processes, carry on the basis of alcohol deposition method adding alkali, introduced plant Chinese medicine clarification technique realizes the extraction purification of high-quality yellow astragalus polysaccharides, the clarification dedoping step introduced efficiently can remove the protein in astragalus polysaccharides, nucleic acid, the macromole impurity such as starch, and improve extracts active ingredients productive rate in astragalus polysaccharides, method astragalus polysaccharides extraction yield after improvement is more than 10.6%, reach as high as 13.5%, the astragalus polysaccharides obtained can be used for regulating animal immune in veterinary clinic, antiviral, the pharmacological agenies such as antibiosis.
Astragalus polysaccharides of the present invention is admixed in the feed for nursing of animal according to certain ratio, a meal is fed in three meals in a day, the immunizing power of animal can be significantly improved, sickness rate has been reduced to 20.5% from initial 32%, the health realizing animal is raised, reduce the treatment of microbiotic, hormonal medicaments, realize ecological raising, improve livestock-raising economic worth.
Embodiment
Following examples describe the present invention and utilize alkalinity extraction technology to produce the technique of astragalus polysaccharides, and these descriptions are not be further limited content of the present invention, and all reagent used in following examples are all bought by commercial means.
embodiment 1
The present embodiment is with the dry Radix Astragali for raw material, and preparation method's step comprises:
1) crude extract obtains: dry astragalus root raw material 1kg, cleaning, dry, pulverize after add 5 times of water (cleaning be for by clean water 3 times, pulverize as astragalus membranaceus powder is broken to 50 orders), add 2 times of alkali solution liquids, alkali solution liquid is leached after constant temperature soaks 2 hours under 50 DEG C of conditions, repeat extraction 3 times, united extraction liquid leaves standstill and cools, and obtains crude extract; Alkali solution liquid is the mixing solutions of sodium carbonate, sodium bisulfite, calcium chloride, and sodium carbonate, sodium bisulfite, the calcium chloride content in leaching solution is respectively 30%, 15%, 40%.
2) concentration and evaporation: add the natural botanical extraction liquid finings KBT-ZTC produced by Ke Lier bio tech ltd in the crude extract that step 1) obtains, add-on is 5g/L, after leaving standstill 30min, rotary evaporation concentrates crude extract, is the medicinal extract of 1.50, obtains medicinal extract crude product to water proportion d;
3) removal of impurities is dissolved: in step 2) add ethanol in the medicinal extract crude product that obtains, the add-on of ethanol is 5 times of volumes of medicinal extract, stir medicinal extract to dissolve, staticly settle, centrifugal segregation supernatant liquor is precipitated thing, throw out adds ethanol again to carry out leaving standstill after stirring, and centrifugal collecting precipitate, obtains sterling astragalus polysaccharides.
Originally be the extraction yield of embodiment astragalus polysaccharides be 12.8%.
embodiment 2
1) crude extract obtains: dry astragalus root raw material 1kg, cleaning, dry, pulverize after add 10 times of water (cleaning be for by clean water 2 times, pulverize as astragalus membranaceus powder is broken to 100 orders), add 3 times of alkali solution liquids, alkali solution liquid is leached after constant temperature soaks 3 hours under 40 DEG C of conditions, repeat extraction 3 times, united extraction liquid leaves standstill and cools, and obtains crude extract; Alkali solution liquid is the mixing solutions of sodium carbonate, sodium bisulfite, calcium chloride, and sodium carbonate, sodium bisulfite, the calcium chloride content in leaching solution is respectively 15%, 30%, 10%.
2) concentration and evaporation: add the natural botanical extraction liquid finings KBT-ZTC produced by Ke Lier bio tech ltd in the crude extract that step 1) obtains, add-on is 10g/L, after leaving standstill 20min, rotary evaporation concentrates crude extract, is the medicinal extract of 1.0, obtains medicinal extract crude product to water proportion d;
3) removal of impurities is dissolved: in step 2) add ethanol in the medicinal extract crude product that obtains, the add-on of ethanol is 2 times of volumes of medicinal extract, stir medicinal extract to dissolve, staticly settle, centrifugal segregation supernatant liquor is precipitated thing, throw out adds ethanol again to carry out leaving standstill after stirring, and centrifugal collecting precipitate, obtains sterling astragalus polysaccharides.
Originally be the extraction yield of embodiment astragalus polysaccharides be 12.5%.
embodiment 3
1) crude extract obtains: dry astragalus root raw material 1kg, cleaning, dry, pulverize after add 8 times of water (cleaning be for by clean water 3 times, pulverize as astragalus membranaceus powder is broken to 75 orders), add 3 times of alkali solution liquids, alkali solution liquid is leached after constant temperature soaks 3 hours under 45 DEG C of conditions, repeat extraction 3 times, united extraction liquid leaves standstill and cools, and obtains crude extract; Alkali solution liquid is the mixing solutions of sodium carbonate, sodium bisulfite, calcium chloride, and sodium carbonate, sodium bisulfite, the calcium chloride content in leaching solution is respectively 20%, 20%, 30%.
2) concentration and evaporation: add the natural botanical extraction liquid finings KBT-ZTC produced by Ke Lier bio tech ltd in the crude extract that step 1) obtains, add-on is 7.5g/L, after leaving standstill 25min, rotary evaporation concentrates crude extract, is the medicinal extract of 1.20, obtains medicinal extract crude product to water proportion d;
3) removal of impurities is dissolved: in step 2) add ethanol in the medicinal extract crude product that obtains, the add-on of ethanol is 3 times of volumes of medicinal extract, stir medicinal extract to dissolve, staticly settle, centrifugal segregation supernatant liquor is precipitated thing, throw out adds ethanol again to carry out leaving standstill after stirring, and centrifugal collecting precipitate, obtains sterling astragalus polysaccharides.
Originally be the extraction yield of embodiment astragalus polysaccharides be 13.5%.
application Example 1
Astragalus polysaccharides is admixed in the feed for nursing of pig according to certain ratio and (is added 50g astragalus polysaccharides in the feed of 1kg), a meal is fed in three meals in a day, the immunizing power of animal can be significantly improved, sickness rate has been reduced to 18.5% from initial 30%, the health realizing animal is raised, reduce the treatment of microbiotic, hormonal medicaments, realize ecological raising, improve livestock-raising economic worth.
application Example 2
Astragalus polysaccharides is admixed in the feed for nursing of ox according to certain ratio and (is added 65g astragalus polysaccharides in the feed of 1kg), a meal is fed in three meals in a day, the immunizing power of animal can be significantly improved, sickness rate has been reduced to 20.5% from initial 32%, the health realizing animal is raised, reduce the treatment of microbiotic, hormonal medicaments, realize ecological raising, improve livestock-raising economic worth.
The above embodiment of the present invention scheme is only can not limit the present invention to explanation of the present invention, scope of the present invention is indicated in claim, and scope of the present invention is not pointed out in above-mentioned explanation, therefore, any change in the implication suitable with claims of the present invention and scope, is all considered to be and is included in the scope of claims.
The present invention is through multidigit astragalus polysaccharides and extracts processing staff's long term operation experience accumulation, and created by creative work, it is in the leaching process at actual astragalus polysaccharides, it is more than 13.5% that the method can obtain astragalus polysaccharides extraction yield, the chemoprophylaxis treatments such as the astragalus polysaccharides obtained can be used for regulating animal immune in veterinary clinic, antiviral, antibiosis.
Claims (6)
1. utilize alkalinity extraction technology to produce a technique for astragalus polysaccharides, be raw material with the Radix Astragali, it is characterized in that, the method step comprises:
1) crude extract acquisition: dry astragalus root raw material 1kg, after cleaning, drying, pulverizing, adds 2 ~ 3 times of alkali solution liquids, and under 40 ~ 50 DEG C of conditions, constant temperature soaks after 2 ~ 3 hours and leaches alkali solution liquid, repeats extraction 3 times, and merging alkali solution liquid liquid leaves standstill and cools, and obtains crude extract;
2) concentration and evaporation: add natural botanical extraction liquid finings KBT-ZTC in the crude extract that step 1) obtains, add-on is 5 ~ 10g/L, and after leaving standstill 20 ~ 30min, rotary evaporation concentrates crude extract, obtains medicinal extract crude product;
3) removal of impurities is dissolved: in step 2) add ethanol in the medicinal extract crude product that obtains, stir medicinal extract and dissolve, staticly settle, centrifugal segregation supernatant liquor is precipitated thing, throw out adds ethanol again to carry out leaving standstill after stirring, and centrifugal collecting precipitate, obtains sterling astragalus polysaccharides.
2. the technique utilizing alkalinity extraction technology to produce astragalus polysaccharides according to claim 1, is characterized in that, described cleaning is for using clean water 2 ~ 3 times; Described pulverizing is that astragalus membranaceus powder is broken to 50 ~ 100 orders.
3. the technique utilizing alkalinity extraction technology to produce astragalus polysaccharides according to claim 1, it is characterized in that, it is the medicinal extract of 1.0 ~ 1.50 that crude extract is evaporated to water proportion d by described rotary evaporation simmer down to.
4. the technique utilizing alkalinity extraction technology to produce astragalus polysaccharides according to claim 1, it is characterized in that, in described step 3), the add-on of ethanol is 2 ~ 5 times of volumes of medicinal extract.
5. the technique utilizing alkalinity extraction technology to produce astragalus polysaccharides according to claim 1, it is characterized in that, described alkali solution liquid is the mixing solutions of sodium carbonate, sodium bisulfite, calcium chloride, and sodium carbonate, sodium bisulfite, the calcium chloride content in leaching solution is respectively 15 ~ 30%, 15 ~ 30%, 10 ~ 40%.
6. the technique utilizing alkalinity extraction technology to produce astragalus polysaccharides according to claim 1, it is characterized in that, described natural botanical extraction liquid finings KBT-ZTC is the finings that You Ke Lier bio tech ltd produces.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106632715A (en) * | 2016-12-19 | 2017-05-10 | 杨凌萃健生物工程技术有限公司 | Lyceum barbarum polysaccharide extracts and preparing method thereof |
| RU2706697C1 (en) * | 2018-12-28 | 2019-11-20 | Федеральное государственное бюджетное учреждение науки Федеральный исследовательский центр "КОМИ научный центр Уральского отделения Российской академии наук" | Extract of astragalus roots |
| CN114948792A (en) * | 2022-06-23 | 2022-08-30 | 伊深细胞生物技术(上海)有限公司 | Preparation method of wash-free smearing type stock solution for hair growth and hair loss prevention |
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| CN101139402A (en) * | 2007-10-18 | 2008-03-12 | 西北大学 | Method for extracting polysaccharide from jujube |
| CN104231102A (en) * | 2014-09-16 | 2014-12-24 | 贵州信邦制药股份有限公司 | Method for extracting polysaccharide ingredient from astragalus membranaceus and glossy privet fruit composition by alkaline extraction and alcohol deposition method and product produced by method |
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Patent Citations (2)
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| CN101139402A (en) * | 2007-10-18 | 2008-03-12 | 西北大学 | Method for extracting polysaccharide from jujube |
| CN104231102A (en) * | 2014-09-16 | 2014-12-24 | 贵州信邦制药股份有限公司 | Method for extracting polysaccharide ingredient from astragalus membranaceus and glossy privet fruit composition by alkaline extraction and alcohol deposition method and product produced by method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106632715A (en) * | 2016-12-19 | 2017-05-10 | 杨凌萃健生物工程技术有限公司 | Lyceum barbarum polysaccharide extracts and preparing method thereof |
| RU2706697C1 (en) * | 2018-12-28 | 2019-11-20 | Федеральное государственное бюджетное учреждение науки Федеральный исследовательский центр "КОМИ научный центр Уральского отделения Российской академии наук" | Extract of astragalus roots |
| CN114948792A (en) * | 2022-06-23 | 2022-08-30 | 伊深细胞生物技术(上海)有限公司 | Preparation method of wash-free smearing type stock solution for hair growth and hair loss prevention |
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