CN103980335B - The preparation method of Momordica grosvenori mogroside V - Google Patents
The preparation method of Momordica grosvenori mogroside V Download PDFInfo
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Abstract
The invention belongs to Momordica grosvenori manufacture field, specifically a kind of preparation method of Momordica grosvenori mogroside V. It is initiation material that this preparation method utilizes ripe Momordica grosvenori fresh fruit, through broken, extract, clarification removal of impurities, filtration, macroporous absorbent resin absorption, wash-out, the absorption of secondary ion exchanger resin, concentrated, dry, the refining Momordica grosvenori mogroside V that obtains of ethanol, in extraction, adopt nontoxic ethanol to replace traditional methyl alcohol, in leaching process, introduce new type natural Chinese medicine fining agent and remove the impurity such as pectin, fiber, protein, in elution process, adopt two kinds of different polymeric adsorbent absorption. The present invention utilizes ethanol to replace traditional methyl alcohol, has reduced the extent of injury of solvent to health, and after fining agent removal of impurities, twice upper prop absorption are purified, product purity is increased to more than 97%.
Description
Technical field
The invention belongs to Momordica grosvenori manufacture field, specifically a kind of preparation method of Momordica grosvenori mogroside V.
Background technology
Momordica grosvenori is perennial climing plant, and Main Cultivation is at Chinese In Northern Guangxi. Its dry fruit is oval or circular, outerShell sepia or smoking color also have a large amount of tiny black fine, soft furs. Utilize its sweet taste and as Chinese medicine existing number centuries Chinese peopleHistorical. After processing, Momordica grosvenori can be used as the medicine for the treatment of flu and moistening lung. Modern Momordica grosvenori generally by as fruit drink orLow-calorie sweetener in beverage uses or is made into good to eat beverage. Mainly concentrate on for the research and development of Momordica grosvenori at presentOn sweet glucoside, wherein Momordica grosvenori mogroside V is main sweet ingredient in sweet glucoside. The sugariness of sweet glucoside be sugar 300 times and thermal content low.That diabetic is stable and without fermentable perfect additive. To replace artificial sweetening agent as a kind of general natural sweetenerAs Aspartame etc. obtains desirable substitute. At beverage, baked goods, nutraceutical, low-heat food or other needs are low or carbon-freeSweet maybe needs of hydrate taste can be given full play to its effect in low or empty calory edible food. Culinary art or baking can't affect its windTaste or sugariness.
At present, the method and the report that extract Momordica grosvenori mogroside V are a lot, but are all taking new fresh fructus momordicae as raw material, pass through water extractionThe techniques such as enzymolysis upper prop purifying, water extraction film concentration extraction are produced. Preparation method's research of Momordica grosvenori mogroside V, as patentCN200710050255.9, discloses a kind of preparation method of high-purity Momordica grosvenori mogroside V, and its step is as follows: 1) get Momordica grosvenori sweetGlucoside is dissolved in methyl alcohol, filters, and obtains filtrate; 2) in gained filtrate, add acetone to make it produce precipitation, filter, obtain sediment;3) taking precipitate adsorbs with normal phase silicagel column; 4) normal phase silicagel column having adsorbed, with the mixed solution of low carbon chain ester and ethanolFor eluant, eluent carries out wash-out, collect eluent; 5) reclaim the eluant, eluent in eluent, crystallization, obtains high-purity Momordica grosvenori mogrosideV, this invention need to adopt expensive normal phase silicagel column, and the wash-out of beet aglucone V need to rely on low carbon chain ester, makes thisExplained hereafter cost is high. As patent CN201010223923.5, disclose that a kind of from dried fructus momordicae, to extract high-load Momordica grosvenori sweetThe method of glucoside V, extraction process comprises the steps: to extract, concentrated, filtration, absorption, decolouring, concentrate drying must contain arhatFruit beet aglucone V product, it adopts ethanol in extraction step is solvent, but this invention is to carrying out removal of impurities in leaching process, obtainsTo product in there is the impurity of fiber, albumen, pectin one class, product purity is not high.
Summary of the invention
The object of the invention is to overcome existing Momordica grosvenori mogroside V extraction process and have extraction removal of impurities difficulty, leaching organic solution methyl alcoholPoisonous, the problem such as utilization rate of equipment and installations is low, product purity is low, production cost is high, provides a kind of preparation method of Momordica grosvenori mogroside V.
The solution of the present invention is by such realization: a kind of preparation method of Momordica grosvenori mogroside V, chooses that fresh nothing goes rottenRipe Momordica grosvenori, the method step comprises:
Step 1) extract: get ripe arhat fresh fruit and carry out fragmentation, with the Momordica grosvenori extracting after solvent counter-flow extraction or refluxing extraction fragmentation,Ratio=2～the 8L:1kg of extractant and Momordica grosvenori, extraction time is 3～8 hours, obtains extract.
Step 2) clarification removal of impurities: in step 1) add fining agent, the ratio of fining agent and extract in the extract that obtains=0.05～0.1kg:1L, after stirring, leaves standstill 25～40min, removes by filter precipitation, obtains the solution after removal of impurities.
Step 3) upper prop absorption: by step 2) solution that obtains after removal of impurities adsorbs by macroporous absorbent resin, resin filling is highSpend 1.5～2 meters, extract solution absorption flow velocity 1-4BV/hr, adsorbed the rear eluting solvent wash-out of using, eluant, eluent and extraction solutionRatio=0.3～0.6L:1L, collects eluent I.
Step 4) secondary upper prop absorption: by step 3) the eluent I that obtains adsorbs by ion exchange resin, after having adsorbedUse eluting solvent wash-out, eluant, eluent and extraction solution proportion=0.1～0.4L:1L, collect eluent II.
Step 5) concentrate drying is refining: by step 4) the eluent II that obtains adopts traditional vacuum concentrate drying, what vacuumizeIn situation, centrifugal speed is that 300～800rpm, temperature are that under the condition of 30～37 DEG C, concentrate drying is to beet aglucone V product water content≤ 10%, more refining through ethanol, obtain beet aglucone V product.
As further restriction of the present invention, while adopting refluxing extraction in described extraction step, refluxing extraction divides to enter for 2～3 timesOK, each 2 hours; Described extractant be in 40～80% ethanol water, pure water any.
As further restriction of the present invention, the fining agent in described clarification removal step is natural traditional Chinese medicine fining agentKBT-ZTC; Described step 3) and 4) in the eluant, eluent used be any in ethanol or the aqueous solution.
As further restriction of the present invention, described step 5) in ethanol refine as utilizing ethanol to dissolve completely after dryThe beet aglucone V arriving, removes by filter sediment, and concentrate drying is to beet aglucone V product water content≤10% again.
As complete scheme of the present invention, the method step comprises:
Step 1) extract: get ripe arhat fresh fruit and carry out fragmentation, with the arhat extracting after solvent 60% ethanol water refluxing extraction fragmentationFruit 3 times, the ratio=6L:1kg of extractant and Momordica grosvenori, each extraction time is 2.5 hours, obtains extract.
Step 2) clarification removal of impurities: in step 1) add fining agent, the ratio of fining agent and extract in the extract that obtains=0.05～0.1kg:1L, after stirring, leaves standstill 25～40min, removes by filter precipitation, obtains the solution after removal of impurities.
Step 3) upper prop absorption: by step 2) solution that obtains after removal of impurities adsorbs by macroporous absorbent resin, resin filling is highSpend 2 meters, extract solution absorption flow velocity 2.5BV/hr, adsorbed the rear eluting solvent wash-out of using, eluant, eluent and extraction solution proportion=0.5L:1L, collects eluent I.
Step 4) secondary upper prop absorption: by step 3) the eluent I that obtains adsorbs by ion exchange resin, after having adsorbedUse eluting solvent wash-out, eluant, eluent and extraction solution proportion=0.2L:1L, collect eluent II.
Step 5) concentrate drying is refining: by step 4) the eluent II that obtains adopts traditional vacuum concentrate drying, what vacuumizeIn situation, centrifugal speed is that 500rpm, temperature are under the condition of 37 DEG C, and concentrate drying is to beet aglucone V product water content≤10%,After dissolving beet aglucone V completely with 60% ethanol water again, remove by filter precipitation, then concentrate drying to beet aglucone V product water content≤10%, obtain beet aglucone V product.
The know-why that the present invention realizes is: utilizing ripe Momordica grosvenori fresh fruit is initiation material, removes through broken, extraction, clarificationAssorted, filtration, macroporous absorbent resin absorption, wash-out, the absorption of secondary ion exchanger resin, concentrated, dry, ethanol is refining obtainsMomordica grosvenori mogroside V adopts nontoxic ethanol to replace traditional methyl alcohol in extraction, reduces the injury of organic solvent to workman, and is carryingGet in process and introduce new type natural Chinese medicine fining agent, effectively remove the impurity such as pectin, fiber, protein, in elution process, adoptWith two kinds of different polymeric adsorbent absorption, improve the purity of product.
The present invention possesses following good result:
(1) the present invention adds new type natural Chinese medicine fining agent in extract, and flocculation sediment has been removed the pectin in extract, fibreThe impurity such as dimension, protein, make to extract purifying and have obtained purifying, have solved a difficult problem for this product extracting liquid filtering technology, have improvedCapacity utilization, has reduced equipment use cost, has shortened filtration time and make the most of impurity in extract obtain effectively simultaneouslyRemove.
(2) by the absorption of different polymeric adsorbents and twice different adsorpting column chromatography wash-out of different eluents, make productPurity be greatly improved, purity reaches more than 97%.
(3) the present invention adopts ethanol to replace methyl alcohol to refine, and has reduced the extent of injury of organic solvent to health.
Brief description of the drawings
Fig. 1 is preparation method's flow chart of the present invention.
Detailed description of the invention
The preparation method who describes a kind of Momordica grosvenori mogroside V of the present invention below in conjunction with embodiment and accompanying drawing, these descriptions are not rightContent of the present invention is further limited.
Embodiment 1
A preparation method for Momordica grosvenori mogroside V, the method step comprises:
Step 1) extract: get ripe arhat fresh fruit and carry out fragmentation, with the Momordica grosvenori extracting after solvent counter-flow extraction or refluxing extraction fragmentation,Ratio=the 8L:1kg of extractant and Momordica grosvenori, extraction time is 5 hours, obtains extract;
Step 2) clarification removal of impurities: in step 1) and add fining agent in the extract that obtains, the ratio=0.1kg:1L of fining agent and extract,After stirring, leave standstill 40min, remove by filter precipitation, obtain the solution after removal of impurities;
Step 3) upper prop absorption: by step 2) solution that obtains after removal of impurities adsorbs by macroporous absorbent resin, 1.8 meters of resin loading heights,Extract solution absorption flow velocity 4BV/hr, adsorbed the rear eluting solvent wash-out of using, eluant, eluent and extraction solution proportion=0.5L:1L,Collect eluent I;
Step 4) secondary upper prop absorption: by step 3) the eluent I that obtains adsorbs by ion exchange resin, after having adsorbed, uses wash-outSolvent elution, eluant, eluent and extraction solution proportion=0.3L:1L, collect eluent II;
Step 5) concentrate drying is refining: by step 4) the eluent II that obtains adopts traditional vacuum concentrate drying, in the situation that vacuumizing,Centrifugal speed is that 600rpm, temperature are under the condition of 37 DEG C, and concentrate drying is to beet aglucone V product water content≤10%, then passes throughEthanol is refining, obtains beet aglucone V product.
While adopting refluxing extraction in extraction step, refluxing extraction is divided and is carried out for 3 times, each 2 hours; Described extractant is 80%Ethanol water.
Fining agent in clarification removal step is natural traditional Chinese medicine fining agent KBT-ZTC; Described step 3) and 4) in use washDe-agent is ethanol.
Step 5) in ethanol refine as utilizing ethanol to dissolve the beet aglucone V obtaining after dry completely, remove by filter sediment, thenInferior concentrate drying is to beet aglucone V product water content≤10%.
The present embodiment obtains product purity and reaches 97.5%.
Embodiment 2
A preparation method for Momordica grosvenori mogroside V, the method step comprises:
Step 1) extract: get ripe arhat fresh fruit and carry out fragmentation, with the Momordica grosvenori extracting after solvent counter-flow extraction or refluxing extraction fragmentation,Ratio=the 2L:1kg of extractant and Momordica grosvenori, extraction time is 8 hours, obtains extract;
Step 2) clarification removal of impurities: in step 1) add fining agent, the ratio=0.05kg of fining agent and extract in the extract that obtains:1L, after stirring, leaves standstill 25min, removes by filter precipitation, obtains the solution after removal of impurities;
Step 3) upper prop absorption: by step 2) solution that obtains after removal of impurities adsorbs by macroporous absorbent resin, 2 meters of resin loading heights,Extract solution absorption flow velocity 3BV/hr, adsorbed the rear eluting solvent wash-out of using, eluant, eluent and extraction solution proportion=0.3L:1L,Collect eluent I;
Step 4) secondary upper prop absorption: by step 3) the eluent I that obtains adsorbs by ion exchange resin, after having adsorbed, uses wash-outSolvent elution, eluant, eluent and extraction solution proportion=0.1L:1L, collect eluent II;
Step 5) concentrate drying is refining: by step 4) the eluent II that obtains adopts traditional vacuum concentrate drying, in the situation that vacuumizing,Centrifugal speed is that 300rpm, temperature are under the condition of 30 DEG C, and concentrate drying is to beet aglucone V product water content≤10%, then passes throughEthanol is refining, obtains beet aglucone V product.
While adopting refluxing extraction in extraction step, refluxing extraction is divided and is carried out for 2 times, each 2 hours; Described extractant is 40%Ethanol water.
Fining agent in clarification removal step is natural traditional Chinese medicine fining agent KBT-ZTC; Described step 3) and 4) in use washDe-agent is ethanol.
Step 5) in ethanol refine as utilizing ethanol to dissolve the beet aglucone V obtaining after dry completely, remove by filter sediment, thenInferior concentrate drying is to beet aglucone V product water content≤10%.
The present embodiment obtains product purity and reaches 98.5%.
Embodiment 3
A preparation method for Momordica grosvenori mogroside V, the method step comprises:
Step 1) extract: get ripe arhat fresh fruit and carry out fragmentation, with the Momordica grosvenori extracting after solvent counter-flow extraction or refluxing extraction fragmentation,Ratio=the 6L:1kg of extractant and Momordica grosvenori, extraction time is 3 hours, obtains extract;
Step 2) clarification removal of impurities: in step 1) add fining agent, the ratio=0.08kg of fining agent and extract in the extract that obtains:1L, after stirring, leaves standstill 25min, removes by filter precipitation, obtains the solution after removal of impurities;
Step 3) upper prop absorption: by step 2) solution that obtains after removal of impurities adsorbs by macroporous absorbent resin, 1.5 meters of resin loading heights,Extract solution absorption flow velocity 1BV/hr, adsorbed the rear eluting solvent wash-out of using, eluant, eluent and extraction solution proportion=0.3L:1L,Collect eluent I;
Step 4) secondary upper prop absorption: by step 3) the eluent I that obtains adsorbs by ion exchange resin, after having adsorbed, uses wash-outSolvent elution, eluant, eluent and extraction solution proportion=0.1L:1L, collect eluent II;
Step 5) concentrate drying is refining: by step 4) the eluent II that obtains adopts traditional vacuum concentrate drying, in the situation that vacuumizing,Centrifugal speed is that 300rpm, temperature are under the condition of 35 DEG C, and concentrate drying is to beet aglucone V product water content≤10%, then passes throughEthanol is refining, obtains beet aglucone V product.
While adopting refluxing extraction in extraction step, refluxing extraction is divided and is carried out for 3 times, each 2 hours; Described extractant is 60%Ethanol water.
Fining agent in clarification removal step is natural traditional Chinese medicine fining agent KBT-ZTC; Described step 3) and 4) in use washDe-agent is the aqueous solution.
Step 5) in ethanol refine as utilizing ethanol to dissolve the beet aglucone V obtaining after dry completely, remove by filter sediment, thenInferior concentrate drying is to beet aglucone V product water content≤10%.
The present embodiment obtains product purity and reaches 98.2%.
Embodiment 4
A preparation method for Momordica grosvenori mogroside V, the method step comprises:
Step 1) extract: get ripe arhat fresh fruit and carry out fragmentation, with the Momordica grosvenori extracting after solvent counter-flow extraction or refluxing extraction fragmentation,Ratio=the 4L:1kg of extractant and Momordica grosvenori, extraction time is 5 hours, obtains extract;
Step 2) clarification removal of impurities: in step 1) add fining agent, the ratio=0.05kg of fining agent and extract in the extract that obtains:1L, after stirring, leaves standstill 25～40min, removes by filter precipitation, obtains the solution after removal of impurities;
Step 3) upper prop absorption: by step 2) solution that obtains after removal of impurities adsorbs by macroporous absorbent resin, 1.6 meters of resin loading heights,Extract solution absorption flow velocity 2.5BV/hr, adsorbed the rear eluting solvent wash-out of using, eluant, eluent and extraction solution proportion=0.45L:1L,Collect eluent I;
Step 4) secondary upper prop absorption: by step 3) the eluent I that obtains adsorbs by ion exchange resin, after having adsorbed, uses wash-outSolvent elution, eluant, eluent and extraction solution proportion=0.25L:1L, collect eluent II;
Step 5) concentrate drying is refining: by step 4) the eluent II that obtains adopts traditional vacuum concentrate drying, in the situation that vacuumizing,Centrifugal speed is that 600rpm, temperature are under the condition of 30 DEG C, and concentrate drying is to beet aglucone V product water content≤10%, then passes throughEthanol is refining, obtains beet aglucone V product.
While adopting refluxing extraction in extraction step, refluxing extraction is divided and is carried out for 3 times, each 2 hours; Described extractant is pureWater.
Fining agent in clarification removal step is natural traditional Chinese medicine fining agent KBT-ZTC; Described step 3) and 4) in use washDe-agent is ethanol.
Step 5) in ethanol refine as utilizing ethanol to dissolve the beet aglucone V obtaining after dry completely, remove by filter sediment, thenInferior concentrate drying is to beet aglucone V product water content≤10%.
The present embodiment obtains product purity and reaches 97.8%.
Embodiment 5
A preparation method for Momordica grosvenori mogroside V, the method step comprises:
Step 1) extract: get ripe arhat fresh fruit and carry out fragmentation, with the arhat extracting after solvent 60% ethanol water refluxing extraction fragmentationFruit 3 times, the ratio=6L:1kg of extractant and Momordica grosvenori, each extraction time is 2.5 hours, obtains extract;
Step 2) clarification removal of impurities: in step 1) add fining agent, the ratio=0.05kg of fining agent and extract in the extract that obtains:1L, after stirring, leaves standstill 25min, removes by filter precipitation, obtains the solution after removal of impurities;
Step 3) upper prop absorption: by step 2) solution that obtains after removal of impurities adsorbs by macroporous absorbent resin, 2 meters of resin loading heights,Extract solution absorption flow velocity 2.5BV/hr, adsorbed the rear eluting solvent wash-out of using, eluant, eluent and extraction solution proportion=0.5L:1L,Collect eluent I;
Step 4) secondary upper prop absorption: by step 3) the eluent I that obtains adsorbs by ion exchange resin, after having adsorbed, uses wash-outSolvent elution, eluant, eluent and extraction solution proportion=0.2L:1L, collect eluent II;
Step 5) concentrate drying is refining: by step 4) the eluent II that obtains adopts traditional vacuum concentrate drying, in the situation that vacuumizing,Centrifugal speed is that 500rpm, temperature are under the condition of 37 DEG C, and concentrate drying is to beet aglucone V product water content≤10%, then uses 60%Ethanol water removes by filter precipitation after dissolving beet aglucone V completely, then concentrate drying is to beet aglucone V product water content≤10%, obtainsBeet aglucone V product.
The present embodiment obtains product purity and reaches 98.9%.
Embodiment 6
A preparation method for Momordica grosvenori mogroside V, the method step comprises:
Step 1) extract: get ripe arhat fresh fruit and carry out fragmentation, with the arhat extracting after solvent 60% ethanol water refluxing extraction fragmentationFruit 3 times, the ratio=6L:1kg of extractant and Momordica grosvenori, each extraction time is 2.5 hours, obtains extract;
Step 2) clarification removal of impurities: in step 1) and add fining agent in the extract that obtains, the ratio=0.1kg:1L of fining agent and extract,After stirring, leave standstill 40min, remove by filter precipitation, obtain the solution after removal of impurities;
Step 3) upper prop absorption: by step 2) solution that obtains after removal of impurities adsorbs by macroporous absorbent resin, 2 meters of resin loading heights,Extract solution absorption flow velocity 2.5BV/hr, adsorbed the rear eluting solvent wash-out of using, eluant, eluent and extraction solution proportion=0.5L:1L,Collect eluent I;
Step 4) secondary upper prop absorption: by step 3) the eluent I that obtains adsorbs by ion exchange resin, after having adsorbed, uses wash-outSolvent elution, eluant, eluent and extraction solution proportion=0.2L:1L, collect eluent II;
Step 5) concentrate drying is refining: by step 4) the eluent II that obtains adopts traditional vacuum concentrate drying, in the situation that vacuumizing,Centrifugal speed is that 500rpm, temperature are under the condition of 37 DEG C, and concentrate drying is to beet aglucone V product water content≤10%, then uses 60%Ethanol water removes by filter precipitation after dissolving beet aglucone V completely, then concentrate drying is to beet aglucone V product water content≤10%, obtainsBeet aglucone V product.
The above embodiment of the present invention scheme is only can not limit the present invention to explanation of the present invention, has pointed out this in claimThe scope of invention product constituent, component ratio, preparation method's parameter, and parameter of the present invention is not pointed out in above-mentioned explanationScope, therefore, any change in implication and the scope suitable with claims of the present invention, is all considered to be and comprisesIn the scope of claims.
The present invention is the researcher's long-term work experience accumulation through the preparation method of multidigit Momordica grosvenori mogroside V, and by woundThe property made work is created and is gone out, and product purity reaches more than 97%.
Claims (5)
1. a preparation method for Momordica grosvenori mogroside V, is characterized in that, the method step comprises:
Step 1) extract: get ripe arhat fresh fruit and carry out fragmentation, with the Momordica grosvenori extracting after solvent counter-flow extraction or refluxing extraction fragmentation, the ratio=2 ~ 8L:1kg of extractant and Momordica grosvenori, extraction time is 3 ~ 8 hours, obtains extract;
Step 2) clarification removal of impurities: in step 1) and add fining agent in the extract that obtains, the ratio=0.05 ~ 0.1kg:1L of fining agent and extract, after stirring, standing 25 ~ 40min, removes by filter precipitation, obtains the solution after removal of impurities;
Step 3) upper prop absorption: by step 2) solution that obtains after removal of impurities adsorbs by macroporous absorbent resin, 1.5 ~ 2 meters of resin loading heights, extract solution absorption flow velocity 1-4BV/hr, adsorb the rear eluting solvent wash-out of using, eluant, eluent and extraction solution proportion=0.3 ~ 0.6L:1L, collect eluent I;
Step 4) secondary upper prop absorption: by step 3) the eluent I that obtains adsorbs by ion exchange resin, adsorb the rear eluting solvent wash-out of use, eluant, eluent and extraction solution proportion=0.1 ~ 0.4L:1L, collection eluent II;
Step 5) concentrate drying is refining: by step 4) the eluent II that obtains adopts traditional vacuum concentrate drying, in the situation that vacuumizing, centrifugal speed is that 300 ~ 800rpm, temperature are under the condition of 30 ~ 37 DEG C, concentrate drying is to beet aglucone V product water content≤10%, refining through ethanol again, obtain beet aglucone V product;
Fining agent in described clarification removal step is natural traditional Chinese medicine fining agent KBT-ZTC.
2. the preparation method of Momordica grosvenori mogroside V according to claim 1, is characterized in that, while adopting refluxing extraction in described extraction step, refluxing extraction is divided and carried out for 2 ~ 3 times, each 2 hours; Described extractant be in 40 ~ 80% ethanol water, pure water any.
3. according to the preparation method of the Momordica grosvenori mogroside V described in claim 1 ~ 2 any one, it is characterized in that described step 3) and 4) in the eluant, eluent used be any in ethanol or water.
4. the preparation method of Momordica grosvenori mogroside V according to claim 3, it is characterized in that, described step 5) in ethanol refine as utilizing ethanol to dissolve the beet aglucone V obtaining after dry completely, remove by filter sediment, concentrate drying is to beet aglucone V product water content≤10% again.
5. the preparation method of Momordica grosvenori mogroside V according to claim 1, is characterized in that, the method step comprises:
Step 1) extract: get ripe arhat fresh fruit and carry out fragmentation, the Momordica grosvenori after the 60% ethanol water refluxing extraction fragmentation of use extraction solvent 3 times, the ratio=6L:1kg of extractant and Momordica grosvenori, each extraction time is 2.5 hours, obtains extract;
Step 2) clarification removal of impurities: in step 1) and add fining agent in the extract that obtains, the ratio=0.05 ~ 0.1kg:1L of fining agent and extract, after stirring, standing 25 ~ 40min, removes by filter precipitation, obtains the solution after removal of impurities;
Step 3) upper prop absorption: by step 2) solution that obtains after removal of impurities adsorbs by macroporous absorbent resin, 2 meters of resin loading heights, extract solution absorption flow velocity 2.5BV/hr, have adsorbed the rear eluting solvent wash-out of using, eluant, eluent and extraction solution proportion=0.5L:1L, collect eluent I;
Step 4) secondary upper prop absorption: by step 3) the eluent I that obtains adsorbs by ion exchange resin, adsorb the rear eluting solvent wash-out of use, eluant, eluent and extraction solution proportion=0.2L:1L, collection eluent II;
Step 5) concentrate drying is refining: by step 4) the eluent II that obtains adopts traditional vacuum concentrate drying, in the situation that vacuumizing, centrifugal speed is that 500rpm, temperature are under the condition of 37 DEG C, concentrate drying is to beet aglucone V product water content≤10%, after dissolving beet aglucone V completely with 60% ethanol water again, remove by filter precipitation, concentrate drying, to beet aglucone V product water content≤10%, obtains beet aglucone V product again.
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| CN108276465B (en) * | 2017-03-14 | 2020-01-07 | 桂林莱茵生物科技股份有限公司 | Method for separating and purifying mogroside V by subcritical water desorption technology |
| CN106943447A (en) * | 2017-04-01 | 2017-07-14 | 成都普睿法药物研发有限公司 | A kind of preparation method of Fructus Monordicae extract |
| CN109247561A (en) * | 2018-09-06 | 2019-01-22 | 湖南绿蔓生物科技股份有限公司 | A kind of method and its application preparing Siraitia grosvenorii sweetener composition from Siraitia grosvenorii |
| CN114539330B (en) * | 2022-02-16 | 2023-07-14 | 广西扶南东亚糖业有限公司 | Extraction method and application of mogroside |
| CN115873059B (en) * | 2023-01-05 | 2024-03-26 | 湖南华康生物资源有限公司 | Method for separating mogroside V from fructus Siraitiae Grosvenorii extraction waste liquid |
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| CN101386636A (en) * | 2007-09-14 | 2009-03-18 | 桂林市振达生物科技有限责任公司 | Mogroside extraction method from grosvenor momordica |
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