CN115873059B

CN115873059B - Method for separating mogroside V from fructus Siraitiae Grosvenorii extraction waste liquid

Info

Publication number: CN115873059B
Application number: CN202310022693.3A
Authority: CN
Inventors: 刘星勋; 欧阳勇; 王志成
Original assignee: Hunan Huakang Biological Resources Co ltd
Current assignee: Hunan Huakang Biological Resources Co ltd
Priority date: 2023-01-05
Filing date: 2023-01-05
Publication date: 2024-03-26
Anticipated expiration: 2043-01-05
Also published as: CN115873059A

Abstract

The invention provides a method for separating mogroside V from momordica grosvenori extraction waste liquid, which comprises the following steps: the front flowing liquid and the washing liquid are all collected when the momordica grosvenori is put on the column, and the effluent liquid is all collected when the resin is regenerated; concentrating the collected waste liquid; removing impurities from the obtained concentrated waste liquid; adsorbing clear feed liquid obtained after impurity removal by using a first resin, washing the adsorbed first resin with purified water, eluting with ethanol with a first concentration, eluting with ethanol with a second concentration, and collecting eluents respectively; decolorizing the eluent obtained by eluting with ethanol with a first concentration by using a second resin, and collecting effluent; concentrating the effluent liquid; concentrating the effluent liquid to obtain a concentrated solution, and drying the concentrated solution; the eluent obtained by eluting with ethanol with the second concentration is concentrated and dried separately. The invention separates the mogroside and the momordica flavone from the waste liquid flowing out when the momordica extract passes through the column and the resin regeneration waste liquid, thereby avoiding wasting resources, saving production cost and reducing environmental pollution.

Description

Method for separating mogroside V from fructus Siraitiae Grosvenorii extraction waste liquid

Technical Field

The invention relates to the technical field of extraction of plant components, in particular to a method for separating mogroside V from a momordica grosvenori extraction waste liquid.

Background

Momordica grosvenori, the fruit of perennial vine of Cucurbitaceae. The fruit is named as "the immortal fruit", its leaf-heart shape, hermaphroditic strain, flowering in summer and fruiting in autumn. The method is mainly produced in Guangxi Zhuang nationality autonomous region Gui Linshi Longjiang county, longsheng and Baishou, etc., yong Fu county and Longsheng county are villages of fructus momordicae, the planting history is long, wherein the Yong Fu planted fructus momordicae has a history of over 300 years, the Longsheng county planted fructus momordicae has a history of over 200 years, ninety percent of Chinese fructus momordicae is produced in Yong Fu county and Longsheng county, the fructus momordicae is a rare earth property of Guilin, and the fructus momordicae is one of the first approved medical and edible materials in China, and the main efficacy of the method is that cough relieving and phlegm resolving. The fruit has high nutritive value and contains rich vitamin C (400 mg-500 mg per 100 g fresh fruit), and glucoside, fructose, glucose, protein, lipid, etc. Momordica grosvenori is sweet and cool in taste, enters lung and large intestine meridians, has the effects of moistening lung to arrest cough, promoting fluid production to quench thirst, is suitable for cough due to lung heat or lung dryness, pertussis, thirst due to body fluid impairment due to summer heat and the like, and has the effect of relaxing bowel.

Momordica grosvenori contains mogrosides (esgoside), 300 times sweeter than sucrose; further contains fructose, amino acids, flavone, etc. The fructus Siraitiae Grosvenorii contains non-sugar sweet components mainly including triterpene glycoside: mogroside V and IV 1-3, with the sweetness of glycoside V being 256-344 times that of sucrose (srcose), and glycoside IV being 126 times that of sucrose, and without glycoside IV being sweet; the secondary is D-mannitol (D-mannitol), which has a sweetness of 0.55-0.65 times that of sucrose. Also contains a large amount of glucose (glucose), and fructose (fructose) is 14%. Contains 26 inorganic elements such as manganese, iron, nickel, selenium, tin, iodine, molybdenum, protein, vitamin C (vitamin C), etc. The kernel contains 41.07% of grease, and the fatty acid in the kernel comprises: linoleic acid (linoleic acid), oleic acid (oleic acid), palmitic acid (palmitic acid), stearic acid (stearic acid), palmitoleic acid (palmitoleic acid), myristic acid (mysteric acid), lauric acid (lauric acid), and capric acid (decanoic acid).

In the prior art, mogroside is mainly extracted and separated from momordica grosvenori, and is eluted through resin adsorption, ion resin decolorization (water is used for extraction after the shell of the momordica grosvenori is broken to obtain extracting solution, the extracting solution is centrifuged and filtered to obtain clear filtrate, the clear filtrate is adsorbed by a resin chromatographic column, the resin chromatographic column is eluted after adsorption is finished, and eluent is decolorized by ion resin), so that 10-30% of mogroside V is difficult to control in actual production operation, and most of momordica grosvenori flavone is wasted by pollution discharge.

Disclosure of Invention

The invention provides a method for separating mogroside V from momordica grosvenori extraction waste liquid, which is characterized in that mogroside V and momordica flavone are separated from waste liquid flowing out when the momordica grosvenori extraction liquid passes through a column and resin regeneration waste liquid, so that the resource waste is avoided, the production cost is saved, and the main product yield is improved, thereby solving the problems proposed by the background technology.

In order to solve the technical problems, the invention discloses a method for separating mogroside V from a momordica grosvenori extraction waste liquid, which comprises the following steps:

step S1: collecting waste liquid: the front flowing liquid and the washing liquid are all collected when the momordica grosvenori is put on the column, and the effluent liquid is all collected when the resin is regenerated;

step S2: concentrating the waste liquid collected in the step S1;

step S3: removing impurities from the concentrated waste liquid obtained in the step S2;

step S4: resin adsorption: adsorbing the clear feed liquid obtained after impurity removal in the step S3 by using a first resin, washing the adsorbed first resin with purified water, eluting with ethanol with a first concentration, eluting with ethanol with a second concentration, and collecting eluents respectively;

step S5: decolorizing the eluent obtained by eluting with ethanol with a first concentration by using a second resin, and collecting effluent;

Step S6: concentrating the effluent liquid obtained in the step S5;

step S7: drying the concentrated solution obtained in the step S6; the eluent obtained by eluting with ethanol with the second concentration is concentrated and dried separately.

Preferably, in the step S2: concentrating to 1/2 of the original volume by vacuum;

in the step S6: and (5) concentrating the effluent obtained in the step (S5) and recycling the solvent to 20 Baume degrees by vacuum concentration.

Preferably, the vacuum degree is-0.07 MPa to-0.088 MPa during vacuum concentration, and the concentration temperature is less than 60 ℃.

Preferably, in the step S4, the first concentration of ethanol is 60% ethanol, and the second concentration of ethanol is 75% ethanol.

Preferably, the first resin is a macroporous resin, and the second resin is an ionic resin.

Preferably, the step S3 adopts high-speed centrifugation to remove impurities or flocculation precipitation to remove impurities, the step S7 adopts spray drying or freeze drying to dry, and the steps of crushing, sieving, packaging and warehousing are sequentially carried out after the drying.

Preferably, during spray drying, the air inlet temperature is less than or equal to 180 ℃ and the air outlet temperature is 80-100 ℃;

and the vacuum degree is-0.00 MPa to-0.088 MPa during freeze drying.

Preferably, the step S7 is performed by a multifunctional device, which includes:

The spray drying device comprises a bottom plate and a drying box, wherein the upper end of the bottom plate is fixedly connected with a first bracket, the lower end of the drying box is fixedly connected with the upper end of the first bracket, the lower end of the drying box is provided with a discharging channel, and the drying box is connected with a spraying mechanism;

the crushing and screening box is fixedly connected to the upper end of the bottom plate, a feed inlet of the crushing and screening box is communicated with the discharge channel, a second support is fixedly connected to the upper end of the crushing and screening box, the second support is fixedly connected with the side wall of the drying box, a crushing chamber and a screening chamber are sequentially arranged in the crushing and screening box from top to bottom, a crushing device is arranged in the crushing chamber, and a screening net is arranged in the screening chamber;

the fixed end of the second horizontal telescopic rod is fixedly connected with the side wall of the drying oven through a fifth bracket, the telescopic end of the second horizontal telescopic rod is connected with a horizontal sealing plate, and the horizontal sealing plate penetrates through the right side of the upper part of the discharging channel;

the liquid storage tank, the liquid storage tank sets up in the drying cabinet upper end, the liquid outlet of liquid storage tank is connected with the inlet of delivery pump through first pipeline, the liquid outlet of delivery pump is connected with the second pipeline, second pipeline intercommunication spraying mechanism.

Preferably, a plurality of spraying mechanisms are embedded in the side wall of the drying oven, the spraying mechanism is a multifunctional spraying mechanism, and the multifunctional spraying mechanism comprises:

the box body is embedded in the side wall of the drying box, and a first opening is formed in one side of the box body, which is positioned on the inner side of the drying box;

the U-shaped frame is fixedly connected with a first sliding block at the lower end of the U-shaped frame, the first sliding block is in sliding connection with the inner wall of the lower end of the box body along the left-right direction, one side of the U-shaped frame, which is far away from the first opening, is provided with a horizontal extension section, and the height of one side of the U-shaped frame, which is close to the first opening, is higher than the height of one side of the U-shaped frame, which is far away from the first opening;

the middle part of the lower end of the liquid storage seat is rotationally connected with the upper end of one side, close to the first opening, of the U-shaped frame, the liquid storage seat penetrates through the first opening, a first elastic sealing piece is connected between the part, located in the first opening, of the liquid storage seat and the inner wall of the first opening, a liquid storage chamber is arranged in the liquid storage seat, and the liquid storage chamber is connected with the second pipeline through a hose;

third, third includes: the first vertical section penetrates through the horizontal extension section in a sliding manner along the up-down direction, a first spring is fixedly connected between the first vertical section and the horizontal extension section, and the lower end of the first vertical section is connected with a first matching wheel; the horizontal section is fixedly connected to one side, close to the first opening, of the first vertical section, and the lower end of the horizontal section is connected with a matching block in a sliding manner;

Fourth, the support fourth includes: the upper end of the second vertical section is rotationally connected with the liquid storage seat, the lower end of the second vertical section is fixedly connected with the lower end of the inclined section, the upper end of the inclined section is rotationally connected with the matching block, and the bracket IV is positioned on one side of the U-shaped frame far away from the first opening;

the first sealing plate is hermetically and slidingly connected in the liquid storage chamber along the direction parallel to the axis of the liquid storage chamber, a first connecting rod is vertically and fixedly connected to the first sealing plate, the first connecting rod penetrates through the upper end of the liquid storage seat in a sliding manner, and a second matching wheel is fixedly connected to the upper end of the first connecting rod;

the arc-shaped seat is fixedly connected to the inner wall of one side, far away from the first opening, of the box body, the circle center of the arc-shaped seat is positioned below the arc-shaped seat, and the second matching wheel is used for being in contact with the arc-shaped surface of the arc-shaped seat;

the atomizing nozzle is fixedly connected to one end of the liquid storage seat in the drying box, a liquid flow channel is arranged in the liquid storage seat, and the liquid flow channel is communicated with a liquid inlet of the atomizing nozzle and a liquid outlet of the liquid storage chamber;

the fixed end of the first horizontal electric telescopic rod is fixedly connected to the inner wall of the lower end of the box body, and the telescopic end of the first horizontal electric telescopic rod is fixedly connected with the first sliding block;

The fixed block is fixedly connected to the inner wall of the lower end of the box body, one side, close to the first opening, of the fixed block is provided with a matching inclined plane, and the matching inclined plane is in contact with the first matching wheel;

the support six is fixedly connected with the lower end of the first sliding block, the support six penetrates through one end of the box body, located in the drying box, and the support six is provided with a temperature sensor.

Preferably, the screening net is connected in the screening chamber in a vertical sliding way, the left end of the screening net penetrates through the left side of the screening chamber, the inner side wall of the screening chamber is fixedly provided with a supporting block, the lower ends of the screening net and the supporting block are fixedly connected with a second spring, and the crushing chamber is communicated with the screening chamber through a communication channel; the crushing device comprises:

the crushing roller set is arranged in the crushing chamber and is positioned at a feed inlet of the crushing and screening box;

the left end of the horizontal screw penetrates through the left side of the communication channel, the left end of the horizontal screw is fixedly connected with an output shaft of a second motor, and the second motor is fixedly connected to the side wall of the crushing and screening box;

The two thread sliders are respectively in threaded connection with the two thread segments;

the left end of the left supporting plate is rotationally connected with the left side of the lower end of the crushing chamber, and the right end of the right supporting plate is rotationally connected with the right side of the lower end of the crushing chamber;

the lower ends of the two support rods are respectively and rotatably connected with the upper ends of the two threaded sliding blocks, and the upper ends of the two support rods are respectively and rotatably connected with the left support plate and the right support plate;

the horizontal shaft is rotationally connected with the left side wall of the crushing and screening box, a cam is fixedly connected to the horizontal shaft, the cam is contacted with the upper end of the left part of the screening net, and the horizontal shaft and the horizontal screw are driven by a gear transmission group;

the upper end of the L-shaped guide rod is fixedly connected with the upper end of the crushing and screening box, and the right end of the L-shaped guide rod is fixedly connected with the inner wall of the right side of the crushing and screening box;

the left and right spaced sliding sleeves of the two second connecting rods are sleeved on the horizontal part of the L-shaped guide rod;

the grinding plates are fixedly connected to the lower ends of the two second connecting rods;

the vertical connecting rod penetrates through the upper end of the crushing and screening box in a sliding mode, a second opening is formed in the upper end of the crushing and screening box, the vertical connecting rod is connected in the second opening in a sliding mode, and a second elastic sealing piece is connected between the vertical connecting rod and the second opening;

The fixed end fixed connection of the third horizontal electric telescopic rod is arranged at the upper end of the crushing and screening box, and the telescopic end of the third horizontal electric telescopic rod is fixedly connected with the upper end of the vertical connecting rod.

The beneficial effects of the invention are as follows: the invention separates the mogroside and the momordica flavone from the waste liquid flowing out when the momordica extract passes through the column and the resin regeneration waste liquid, belongs to waste recycling, avoids wasting resources, saves production cost, improves main product yield, and can bring certain economic benefit and social benefit to enterprises and reduce environmental pollution.

The technical scheme of the invention is further described in detail through the drawings and the embodiments.

Drawings

The accompanying drawings are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate the invention and together with the embodiments of the invention, serve to explain the invention. In the drawings:

FIG. 1 is a flow chart of the method of the present invention.

Fig. 2 is a schematic structural diagram of the multifunctional device of the present invention.

Fig. 3 is an enlarged schematic view of the structure at a in fig. 2.

Fig. 4 is an enlarged schematic view of the structure at B in fig. 2.

In the figure: 1. a bottom plate; 2. a drying box; 3. a first bracket; 4. a discharge channel; 5. a spraying mechanism; 51. a case; 52. a first opening; 53. a U-shaped frame; 531. a horizontal extension; 54. a liquid storage seat; 541. a liquid storage chamber; 551. a first vertical section; 552. a first mating wheel; 553. a horizontal section; 554. a mating block; 561. a second vertical section; 562. an inclined section; 57. a first sealing plate; 58. a first connecting rod; 59. a second mating wheel; 510. an arc-shaped seat; 5101. an arc surface; 511. an atomizing nozzle; 512. a fluid passage; 513. a first horizontal electric telescopic rod; 514. a fixed block; 5141. matching with the inclined plane; 515. a sixth bracket; 516. a second opening; 517. a first slider; 6. crushing and screening boxes; 61. a pulverizing chamber; 62. a screening chamber; 7. a second bracket; 8. a pulverizing device; 81. a crushing roller group; 82. a horizontal screw; 83. a second motor; 84. a thread slider; 85. a left support plate; 86. a right support plate; 87. a support rod; 88. a horizontal axis; 89. a cam; 810. an L-shaped guide bar; 811. a second connecting rod; 812. a grinding plate; 813. a vertical connecting rod; 814. a third horizontal electric telescopic rod; 9. screening net; 91. a support block; 10. a second horizontal telescopic rod; 11. a horizontal sealing plate; 12. a communication passage; 13. and a fifth bracket.

Detailed Description

The preferred embodiments of the present invention will be described below with reference to the accompanying drawings, it being understood that the preferred embodiments described herein are for illustration and explanation of the present invention only, and are not intended to limit the present invention.

In addition, the descriptions of the "first," "second," and the like, herein are for descriptive purposes only and are not intended to be specifically construed as order or sequence, nor are they intended to limit the invention solely for distinguishing between components or operations described in the same technical term, but are not to be construed as indicating or implying any relative importance or order of such features. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include at least one such feature. In addition, technical solutions and technical features between the embodiments may be combined with each other, but it is necessary to base that a person skilled in the art can implement the combination of technical solutions, when the combination of technical solutions contradicts or cannot be implemented, should be considered that the combination of technical solutions does not exist, and is not within the scope of protection claimed by the present invention.

The embodiment of the invention provides a method for separating mogroside V from momordica grosvenori extraction waste liquid, which comprises the following steps:

Step S1: collecting waste liquid: the front flowing liquid and the washing liquid are all collected when the momordica grosvenori is put on the column, and the effluent liquid is all collected when the resin is regenerated; (all of the above substances were conventionally discarded in the form of sewage)

Step S2: concentrating the waste liquid collected in the step S1;

step S6: concentrating the effluent liquid obtained in the step S5;

when freeze drying, the vacuum degree is-0.00 MPa to-0.088 MPa, and the temperature is less than-30 ℃ to 30 ℃.

The method of the invention uses the waste liquid generated during the extraction and separation of the momordica grosvenori, separates the mogroside V and the momordica flavone, changes waste into valuable and reduces pollution discharge.

The invention solves the problems of the prior art: in the prior art, mogroside is mainly extracted and separated from momordica grosvenori, is eluted through resin adsorption, is decolorized by ion resin, is difficult to control in actual production operation, and often has the problem that 10-30% of mogroside V can be lost by pollution discharge and most of momordica grosvenori flavone can be wasted by pollution discharge.

The invention specifically includes the following embodiments

Example 1:

a method for separating mogroside V from fructus Siraitiae Grosvenorii extraction waste liquid comprises the following steps:

s1, collecting waste liquid: collecting all effluent liquid before the fructus momordicae is put on the column, washing liquid and resin regeneration; 10KG

S2, concentrating: concentrating in vacuum to about 1/2 of the original volume, about 5KG;

s3, removing impurities: centrifuging the feed liquid at a high speed to remove impurities or flocculating and precipitating to remove impurities;

s4, resin adsorption: about 4.8KG of the clarified feed was adsorbed on macroporous resin (resin model LX 1500), washed with purified water, and eluted with 60% ethanol and 75% ethanol, respectively. Collecting the eluate about 4KG and 2KG respectively; ( And (3) injection: separately concentrating the 75% ethanol eluate and separately collecting the separately dried product )

S5, decoloring: the eluate was decolorized by ion resin and the effluent was collected at about 4.3KG.

S6, concentrating: vacuum concentrating the decolorized solution to recover solvent to about 20 Baume degrees;

s6, drying: spray drying or freeze drying, pulverizing, sieving, packaging, and warehousing.

The content of mogroside V in the product is about 48.2%, the extraction rate of the total mogroside V is 25.6%, and the content of flavone in the momordica grosvenori flavone product is as follows: 32.6%.

Example 2:

S1, collecting waste liquid: collecting all effluent liquid before the fructus momordicae is put on the column, washing liquid and resin regeneration; 5KG

S2, concentrating: concentrating in vacuum to about 1/2 of the original volume, about 2.5KG;

s4, resin adsorption: about 2.4KG of the clarified feed was adsorbed by macroporous resin (resin model LX 1500), washed with purified water, and eluted with 60% ethanol and 75% ethanol, respectively. The eluent was collected at about 2.2KG and 1.1KG (note: 75% ethanol elution collection was collected separately from the concentrated separate dry product)

S5, decoloring: the eluate was decolorized by ion resin and the effluent was collected at about 2.3KG.

The content of mogroside V in the product is about 49.1%, the extraction rate of the total mogroside V is 26.8%, and the content of flavone in the momordica grosvenori flavone product is: 31.2%.

Example 3:

s1, collecting waste liquid: collecting all effluent liquid before the fructus momordicae is put on the column, washing liquid and resin regeneration; 2.5KG;

S2, concentrating: concentrating in vacuum to about 1/2 of the original volume, about 1.25KG;

s4, resin adsorption: about 1.2KG of the clarified feed was adsorbed on macroporous resin (resin model LX 1500), washed with purified water, and eluted with 60% ethanol and 75% ethanol, respectively. Collecting about 1KG and 0.5KG of the eluent respectively; ( And (3) injection: separately concentrating the 75% ethanol eluate and separately collecting the separately dried product )

S5, decoloring: the eluate was decolorized by ion resin and the effluent was collected at about 1KG.

S6, concentrating: vacuum concentrating the decolorized solution to recover solvent to about 20 Baume degree,

The content of mogroside V in the product is about 47.5%, the extraction rate of the total mogroside V is 25.3%, and the flavone content of the momordica grosvenori flavone product is as follows: 30.8%.

Example 4:

s1, collecting waste liquid: collecting all effluent liquid before the fructus momordicae is put on the column, washing liquid and resin regeneration; 20KG

S2, concentrating: concentrating in vacuum to about 10KG of original volume;

s4, resin adsorption: about 9.5KG of the clarified feed was adsorbed on macroporous resin (resin model LX 1500), washed with purified water, and eluted with 60% ethanol and 75% ethanol, respectively. The eluate was collected at about 8.3KG,

4.1KG; ( And (3) injection: separately concentrating the 75% ethanol eluate and separately collecting the separately dried product )

S5, decoloring: the eluate was decolorized by ion resin and the effluent was collected at about 8.5KG.

S6, concentrating: vacuum concentrating the decolorized solution to recover solvent to Baume degree of about 20

The content of mogroside V in the product is about 49.6%, the extraction rate of the total mogroside V is 27.7%, and the content of flavone in the momordica grosvenori flavone product is as follows: 33.4%.

Example 5:

s1, collecting waste liquid: collecting all effluent liquid before the fructus momordicae is put on the column, washing liquid and resin regeneration; 50KG

S2, concentrating: concentrating under vacuum to about 25KG;

s4, resin adsorption: about 24.2KG of the clarified feed was adsorbed on macroporous resin (resin model LX 1500), washed with purified water, and eluted with 60% ethanol and 75% ethanol, respectively. Collecting the eluate about 23KG and 11KG respectively; ( And (3) injection: separately concentrating the 75% ethanol eluate and separately collecting the separately dried product )

S5, decoloring: the eluate was decolorized by ion resin and the effluent was collected at about 24KG.

The content of mogroside V in the product is about 50.4%, the extraction rate of the total mogroside V is 29.3%, and the flavone content of the momordica grosvenori flavone product is as follows: 34.7%.

Example 6:

s1, collecting waste liquid: collecting all effluent liquid before the fructus momordicae is put on the column, washing liquid and resin regeneration; 2KG

S2, concentrating: concentrating under vacuum to about 1KG;

s4, resin adsorption: about 0.96KG of the clarified feed was adsorbed on macroporous resin (resin model LX 1500), washed with purified water, and eluted with 60% ethanol and 75% ethanol, respectively. The eluate was collected at about 0.92KG,

0.45KG; ( And (3) injection: separately concentrating the 75% ethanol eluate and separately collecting the separately dried product )

S5, decoloring: the eluate was decolorized by ion resin and the effluent was collected at about 0.95KG.

The content of mogroside V in the product is about 46.4%, the extraction rate of the total mogroside V is 25.1%, and the content of flavone in the momordica grosvenori flavone product is: 27.6%.

Example 7:

s1, collecting waste liquid: collecting all effluent liquid before the fructus momordicae is put on the column, washing liquid and resin regeneration; 30KG;

s2, concentrating: concentrating under vacuum to about 15KG;

s4, resin adsorption: about 14.5KG of the clarified feed was adsorbed on macroporous resin (resin model LX 1500), washed with purified water, and eluted with 60% ethanol and 75% ethanol, respectively. The eluent is respectively collected at about 16KG and 8.2KG; ( And (3) injection: separately concentrating the 75% ethanol eluate and separately collecting the separately dried product )

S5, decoloring: the eluate was decolorized by ion resin and the effluent was collected at about 15.5KG.

The content of mogroside V in the product is about 49.7%, the extraction rate of the total mogroside V is 28.5%, and the content of flavone in the momordica grosvenori flavone product is as follows: 33.5%.

Example 8:

s1, collecting waste liquid: collecting all effluent liquid before the fructus momordicae is put on the column, washing liquid and resin regeneration; 3KG;

s2, concentrating: concentrating under vacuum to about 1.5KG;

s4, resin adsorption: about 1.4KG of the clarified feed was adsorbed on macroporous resin (resin model LX 1500), washed with purified water, and eluted with 60% ethanol and 75% ethanol, respectively. The eluates were collected at about 1.6KG,

0.8KG; ( And (3) injection: separately concentrating the 75% ethanol eluate and separately collecting the separately dried product )

S5, decoloring: the eluate was decolorized by ion resin and the effluent was collected at about 1.5KG.

The content of mogroside V in the product is about 44.8%, the extraction rate of the total mogroside V is 24.3%, and the content of flavone in the momordica grosvenori flavone product is as follows: 28.4%.

The sewage waste liquid of the mogroside V extracted and separated from the momordica grosvenori mentioned in the above document refers to the waste liquid discharged in the adsorption and elution process in the process of mass production, and the difficulty is how to recycle the waste, thereby obtaining valuable components, improving the use value thereof and making the waste better benefit for mankind.

Device embodiment 1, on the basis of any one of the above embodiments 1 to 9, the step S7 is performed with a multifunctional device including:

the drying device comprises a bottom plate 1 and a drying box 2, wherein the upper end of the bottom plate 1 is fixedly connected with a first bracket 3, the lower end of the drying box 2 is fixedly connected with the upper end of the first bracket 3, a discharging channel 4 is arranged at the lower end of the drying box 2, and the drying box 2 is connected with a spraying mechanism 5;

the crushing and screening box 6 is fixedly connected to the upper end of the bottom plate 1, a feed inlet of the crushing and screening box 6 is communicated with the discharge channel 4, a second support 7 is fixedly connected to the upper end of the crushing and screening box 6, the second support 7 is fixedly connected with the side wall of the drying box 2, a crushing chamber 61 and a screening chamber 62 are sequentially arranged in the crushing and screening box 6 from top to bottom, a crushing device 8 is arranged in the crushing chamber 61, and a screening net 9 is arranged in the screening chamber 62;

The fixed end of the second horizontal telescopic rod 10 is fixedly connected with the side wall of the drying oven 2 through a fifth bracket 13, the telescopic end of the second horizontal telescopic rod 10 is connected with a horizontal sealing plate 11, and the horizontal sealing plate 11 penetrates through the right side of the upper part of the discharging channel 4;

the liquid storage tank, the liquid storage tank sets up in drying cabinet 2 upper end, the liquid outlet of liquid storage tank is connected with the inlet of delivery pump through first pipeline, the liquid outlet of delivery pump is connected with the second pipeline, second pipeline intercommunication spraying mechanism 5.

In this embodiment, the drying box is connected to an existing drying device (such as a hot air drying device), for example, refer to CN 208366042U, and includes: the upper part of the air filter is communicated with a blower, the upper part of the blower is communicated with a steam heater, the upper part of the steam heater is communicated with an electric heater, the upper end of the electric heater is communicated with a high-efficiency filter, and the high-efficiency filter is communicated with the drying oven 2 through an air distributor.

The working principle and beneficial effects of the technical scheme are as follows: before drying, the second horizontal telescopic rod 10 stretches to drive the horizontal sealing plate 11 to move leftwards into the discharging channel 4 until the horizontal sealing plate 11 seals the discharging channel 4; during drying, the concentrated solution obtained in the step S6 is atomized and sprayed into the drying box 2 through the spraying mechanism 5, a drying device such as a hot air drying device is connected in the drying box 2, the atomized concentrated solution and hot air enter the drying box 2 at the same time, and the atomized concentrated solution and the atomized hot air are fully mixed, and the aim of evaporating and drying is fulfilled in a short time due to large heat and mass exchange area, so that the atomized concentrated solution can be dried rapidly. Wherein, in order to avoid the concentrate after the part atomizing not evaporating drying, this part concentrate falls into drying cabinet 2, owing to the closure of above-mentioned horizontal closing plate 11, this part concentrate still remains in drying cabinet 2, be convenient for fully dry, after the final concentrate all finishes drying (also can just open horizontal closing plate 11 once at every turn dry concentrate of a certain amount), open horizontal closing plate 11 for the material after the drying falls into crushing and screening case 6, smash the great material of physical examination through reducing mechanism 8, the material is sieved through screening net 9 after the crushing is accomplished. According to the technical scheme, automatic spray drying, automatic crushing and automatic screening can be realized through one device, and the production efficiency of the invention is improved.

On the basis of the embodiment 1 of the foregoing apparatus, a plurality of spraying mechanisms 5 are embedded in the side wall of the drying oven 2, the spraying mechanisms 5 are multifunctional spraying mechanisms 5, and the multifunctional spraying mechanisms 5 include:

the box body 51 is embedded in the side wall of the drying box 2, and a first opening 52 is formed in one side of the box body 51, which is positioned on the inner side of the drying box 2;

the U-shaped frame 53, the lower end of the U-shaped frame 53 is fixedly connected with a first sliding block, the first sliding block is in sliding connection with the inner wall of the lower end of the box body 51 along the left-right direction, one side of the U-shaped frame 53 far away from the first opening 52 is provided with a horizontal extension section 531, and the height of one side of the U-shaped frame 53 near the first opening 52 is higher than the height of one side of the U-shaped frame 53 far away from the first opening 52;

the middle part of the lower end of the liquid storage seat 54 is rotatably connected with the upper end of one side, close to the first opening 52, of the U-shaped frame 53, the liquid storage seat 54 penetrates through the first opening 52, a first elastic sealing element is connected between the part, located in the first opening 52, of the liquid storage seat 54 and the inner wall of the first opening 52, a liquid storage chamber 541 is arranged in the liquid storage seat 54, and the liquid storage chamber 541 is connected with the second pipeline through a hose;

third, third includes: the first vertical section 551, the first vertical section 551 runs through the horizontal extension section 531 along the up-down direction in a sliding manner, a first spring is fixedly connected between the first vertical section 551 and the horizontal extension section 531, and the lower end of the first vertical section 551 is connected with a first matching wheel 552; the horizontal section 553 is fixedly connected to one side of the first vertical section 551, which is close to the first opening 52, and the lower end of the horizontal section 553 is connected with a matching block 554 in a sliding manner;

Fourth, the support fourth includes: the upper end of the second vertical section 561 is rotationally connected with the liquid storage seat 54, the lower end of the second vertical section 561 is fixedly connected with the lower end of the inclined section 562, the upper end of the inclined section 562 is rotationally connected with the matching block 554, and the bracket four is positioned on one side of the U-shaped frame 53 far away from the first opening 52;

the first sealing plate 57, the first sealing plate 57 is in sealed sliding connection in the liquid storage chamber 541 along the direction parallel to the axis of the liquid storage chamber 541, a first connecting rod 58 is vertically and fixedly connected to the first sealing plate 57, the first connecting rod 58 penetrates through the upper end of the liquid storage seat 54 in a sliding manner, and a second matching wheel 59 is fixedly connected to the upper end of the first connecting rod 58;

the arc-shaped seat 510 is fixedly connected to the inner wall of one side, far away from the first opening 52, of the box body 51, the center of the arc-shaped seat 510 is located below the arc-shaped seat 510, and the second matching wheel 59 is used for contacting with an arc-shaped surface 5101 of the arc-shaped seat 510;

the atomizing nozzle 511, the fixed connection of the said atomizing nozzle 511 is in one end that the stock solution seat 54 locates in dry box 2, set up the fluid channel 512 in the said stock solution seat 54, the said fluid channel 512 communicates the liquid inlet of the said atomizing nozzle 511 and liquid outlet of the said stock solution chamber 541;

The fixed end of the first horizontal electric telescopic rod 513 is fixedly connected to the inner wall of the lower end of the box body 51, and the telescopic end of the first horizontal electric telescopic rod 513 is fixedly connected with the first sliding block 517;

the fixed block 514, the fixed block 514 is fixedly connected to the inner wall of the lower end of the box body 51, one side of the fixed block 514 close to the first opening 52 is provided with a matching inclined surface 5141, and the matching inclined surface 5141 is contacted with the first matching wheel 552;

the support six 515 is fixedly connected with the lower end of the first sliding block 517, the support six 515 penetrates through one end of the box body 51, located in the drying box 2, and a temperature sensor is installed on the support six 515.

The working principle and beneficial effects of the technical scheme are as follows:

in the spraying mechanism 5, during spraying, the concentrated solution obtained in step S6 is input into the liquid storage chamber 541 through the conveying pump and the second pipeline, taking the left spraying mechanism 5 as an example, then the first horizontal electric telescopic rod 513 is controlled to retract to the left, so as to drive the first slider 517 to move to the left (fig. 4 is a diagram moving to the left limit), on the one hand, the first slider 517 moves to the left, the U-shaped frame 53 is driven to move to the left, the atomizing nozzle 511 is driven to move along the left and right directions, the horizontal position of the atomizing nozzle 511 is adjusted, the first matching wheel 552 moves to the left on the matching inclined surface 5141, the first matching wheel 552 and the first vertical section 551 are driven to move upwards, the first vertical section 551 moves upwards, through the second vertical section 561 and the inclined section 562, the left liquid storage seat 54 is driven to rotate around the left U-shaped frame 53, the longitudinal direction of the atomizing nozzle 511 is adjusted, the U-shaped frame 53 drives the liquid storage seat 54 to move to the left until the second matching wheel 59 contacts the arc surface 5101, the arc surface 5101 is driven to squeeze the second matching wheel 59, the second matching wheel 59 drives the first connecting plate 59 to move to the first connecting rod 57, and the sealing plate 541 is pressurized; and the first slider 517 moves left and drives the six supports 515 to move left, so as to drive the temperature sensor to move left to adjust the detection range of the temperature sensor, so that the detection is more reliable, and the temperature of the spraying mechanism 5 or the hot air drying device can be conveniently adjusted according to the detection value of the temperature sensor. According to the technical scheme, the horizontal movement and longitudinal rotation adjusting function of the atomizing nozzle 511 can be realized by controlling the first horizontal electric telescopic rod 513 to be a driving piece, the action range of the atomizing nozzle 511 is adjusted, the condition that the drying efficiency is low due to the fact that the atomizing nozzle 511 acts on only one range is avoided, the pressurizing function of the liquid storage chamber 541 is controlled, the drying efficiency is improved, the detecting range function of the temperature sensor is adjusted, reliable detection is guaranteed, reliable drying is convenient to guarantee, and the drying reliability and the drying efficiency of the invention are improved.

On the basis of the embodiment 1 of the device, the screening net 9 is vertically and slidably connected in the screening chamber 62, the left end of the screening net 9 penetrates through the left side of the screening chamber 62, a supporting block 91 is fixedly arranged on the inner side wall of the screening chamber 62, the screening net 9 is fixedly connected with a second spring at the lower end of the supporting block 91, and the crushing chamber 61 is communicated with the screening chamber 62 through a communication channel 12; the pulverizing device 8 includes:

a crushing roller group 81, wherein the crushing roller group 81 is arranged in the crushing chamber 61 and is positioned at a feed inlet of the crushing and screening box 6;

the horizontal screw 82 is rotationally connected with the left side and the right side of the communication channel 12, the left part and the right part of the horizontal screw 82 positioned in the communication channel 12 are provided with screw thread sections with opposite rotation directions, the left end of the horizontal screw 82 penetrates through the left side of the communication channel 12, the left end of the horizontal screw 82 is fixedly connected with the output shaft of the second motor 83, and the second motor 83 is fixedly connected with the side wall of the crushing and screening box 6;

two thread blocks 84, the two thread blocks 84 are respectively in threaded connection with the two thread segments;

the left end of the left supporting plate 85 is rotationally connected with the left side of the lower end of the crushing chamber 61, and the right end of the right supporting plate 86 is rotationally connected with the right side of the lower end of the crushing chamber 61;

The lower ends of the two support rods 87 are respectively and rotatably connected with the upper ends of the two threaded sliding blocks 84, and the upper ends of the two support rods 87 are respectively and rotatably connected with the left support plate 85 and the right support plate 86;

the horizontal shaft 88 is rotatably connected with the left side wall of the crushing and screening box 6, a cam 89 is fixedly connected to the horizontal shaft 88, the cam 89 is contacted with the upper left part of the screening net 9, and the horizontal shaft 88 and the horizontal screw 82 are driven by a gear transmission group; the gear transmission group is in the prior art and can comprise a driving gear and a driven gear, wherein the driving gear is fixedly sleeved on the horizontal screw 82, the driven gear is fixedly sleeved on the horizontal shaft, and the driving gear is meshed with the driven gear;

the upper end of the L-shaped guide rod 810 is fixedly connected with the upper end of the crushing and screening box 6, and the right end of the L-shaped guide rod 810 is fixedly connected with the right inner wall of the crushing and screening box 6;

two second connecting rods 811 are slidably coupled to the horizontal portion of the L-shaped guide bar 810 at left and right intervals;

the grinding plates 812 are fixedly connected to the lower ends of the two second connecting rods 811;

the vertical connecting rod 813 penetrates through the upper end of the crushing and screening box 6 in a sliding mode, a second opening 516 is formed in the upper end of the crushing and screening box 6, the vertical connecting rod 813 is connected in the second opening 516 in a sliding mode, and a second elastic sealing piece is connected between the vertical connecting rod 813 and the second opening 516;

The third horizontal electric telescopic rod 814, the stiff end fixed connection of third horizontal electric telescopic rod 814 is in smashing and screening case 6 upper end, the flexible end of third horizontal electric telescopic rod 814 and vertical connecting rod 813 upper end fixed connection.

Wherein, the crushing roller set 81 is of the prior art, and will not be described herein, and the driving motor in the crushing roller set 81 is located outside the crushing and screening box 6.

Wherein, optionally, a plurality of grinding protrusions can be arranged at the lower end of the grinding plate 812;

in the initial state, the right side of the left support plate 85 and the left side of the right support plate 86 contact seals (the left support plate 85 and the right support plate 86 are in the horizontal position); the dried materials in the drying box 2 are discharged to the feed inlet of the crushing and screening box 6 through the discharge channel 4, the crushed materials fall to the upper ends of the left support plate 85 and the right support plate 86 through the crushing roller set 81 for one time, the second connecting rod 811 and the grinding plate 812 can be driven to move through the vertical connecting rod 813 by controlling the shrinkage of the third horizontal electric telescopic rod 814, the materials on the left support plate 85 and the right support plate 86 are ground through the grinding plate 812, after the final grinding is finished, the second motor 83 is controlled to work, the second motor 83 drives the horizontal screw 82 to rotate, so that the left and right threaded sliding blocks 84 are mutually far away, the right side of the left support plate 85 and the left side of the right support plate 86 are opened, the ground materials fall to the screening net 9 for screening, the horizontal screw 82 rotates to drive the horizontal shaft 88 through the gear transmission set, the cam 89 on the horizontal shaft 88 rotates, the cam 89 can drive the screening net 9 to move downwards, meanwhile, the screening net 9 can be moved up and down through cooperation with the second spring, and the screening net 9 can be accelerated in a up-down mode, and the working efficiency of the screening machine is improved; and the second motor 83 is continuously controlled to periodically rotate, so that the right side of the left supporting plate 85 and the left side of the right supporting plate 86 are opened for a period of time each time, so that part of materials fall to the screening net 9, and meanwhile, the screening of the part of materials is quickened, and the phenomenon that the materials are accumulated on the screening net 9 once is avoided, so that the screening efficiency and quality are influenced. In the invention, the third horizontal electric telescopic rod 814 is arranged outside the crushing and screening box 6, so that the crushing and screening box is convenient to control, and the powder of materials is prevented from easily influencing the working performance of the third horizontal electric telescopic rod 814. In the invention, the materials screened by the final screening net 9 can be collected manually, and then packaged and put in storage.

It will be apparent to those skilled in the art that various modifications and variations can be made to the present invention without departing from the spirit or scope of the invention. Thus, it is intended that the present invention also include such modifications and alterations insofar as they come within the scope of the appended claims or the equivalents thereof.

Claims

1. A method for separating mogroside V from a waste liquid of extraction of momordica grosvenori, comprising:

step S2: concentrating the waste liquid collected in the step S1;

step S4: resin adsorption: adsorbing the clear feed liquid obtained after impurity removal in the step S3 by using a first resin, washing the adsorbed first resin with purified water, eluting with ethanol with a first concentration, eluting with ethanol with a second concentration, and collecting eluents respectively; in the step S4, the first concentration of ethanol is 60% ethanol, and the second concentration of ethanol is 75% ethanol;

Step S6: concentrating the effluent liquid obtained in the step S5;

step S7: drying the concentrated solution obtained in the step S6; concentrating and drying the eluent obtained by eluting with ethanol with the second concentration;

the first resin is macroporous resin, and the second resin is ionic resin;

the step S7 is performed by a multifunctional apparatus including:

the drying device comprises a bottom plate (1) and a drying box (2), wherein the upper end of the bottom plate (1) is fixedly connected with a first bracket (3), the lower end of the drying box (2) is fixedly connected with the upper end of the first bracket (3), a discharging channel (4) is arranged at the lower end of the drying box (2), and the drying box (2) is connected with a spraying mechanism (5);

crushing and screening case (6), crushing and screening case (6) fixed connection is in bottom plate (1) upper end, crushing and the feed inlet intercommunication of screening case (6) discharge channel (4), crushing and screening case (6) upper end fixedly connected with support two (7), support two (7) with drying cabinet (2) lateral wall fixed connection, crushing and screening case (6) are interior from the top down set gradually crushing room (61) and screening room (62), set up reducing mechanism (8) in crushing room (61), set up screening net (9) in screening room (62);

the fixed end of the second horizontal telescopic rod (10) is fixedly connected with the side wall of the drying oven (2) through a fifth bracket (13), the telescopic end of the second horizontal telescopic rod (10) is connected with a horizontal sealing plate (11), and the horizontal sealing plate (11) penetrates through the right side of the upper part of the discharging channel (4);

The liquid storage tank is arranged at the upper end of the drying box (2), a liquid outlet of the liquid storage tank is connected with a liquid inlet of a conveying pump through a first pipeline, a liquid outlet of the conveying pump is connected with a second pipeline, and the second pipeline is communicated with the spraying mechanism (5);

the lateral wall of drying cabinet (2) is inlayed and is equipped with a plurality of spraying mechanism (5), spraying mechanism (5) are multi-functional spraying mechanism (5), multi-functional spraying mechanism (5) include:

the box body (51) is embedded in the side wall of the drying box (2), and a first opening (52) is formed in one side of the box body (51) positioned at the inner side of the drying box (2);

the U-shaped frame (53), the first slider of fixedly connected with of lower extreme of U-shaped frame (53), first slider and box (51) lower extreme inner wall are along controlling the direction sliding connection, one side that U-shaped frame (53) kept away from first opening (52) sets up horizontal extension section (531), the height of one side that U-shaped frame (53) is close to first opening (52) is higher than the height of one side that U-shaped frame (53) kept away from first opening (52);

the middle part of the lower end of the liquid storage seat (54) is rotationally connected with the upper end of one side, close to the first opening (52), of the U-shaped frame (53), the liquid storage seat (54) penetrates through the first opening (52), a first elastic sealing piece is connected between the part, located in the first opening (52), of the liquid storage seat (54) and the inner wall of the first opening (52), a liquid storage chamber (541) is arranged in the liquid storage seat (54), and the liquid storage chamber (541) is connected with the second pipeline through a hose;

Third, third includes: the first vertical section (551), the first vertical section (551) runs through the horizontal extension section (531) in a sliding manner along the up-down direction, a first spring is fixedly connected between the first vertical section (551) and the horizontal extension section (531), and the lower end of the first vertical section (551) is connected with a first matching wheel (552); the horizontal section (553), the horizontal section (553) is fixedly connected to one side of the first vertical section (551) close to the first opening (52), and the lower end of the horizontal section (553) is connected with a matching block (554) in a sliding manner;

fourth, the support fourth includes: the upper end of the second vertical section (561) is rotationally connected with the liquid storage seat (54), the lower end of the second vertical section (561) is fixedly connected with the lower end of the inclined section (562), the upper end of the inclined section (562) is rotationally connected with the matching block (554), and the bracket is four on one side, far away from the first opening (52), of the U-shaped frame (53);

the first sealing plate (57), the first sealing plate (57) is connected in the liquid storage chamber (541) in a sealing sliding manner along the direction parallel to the axis of the liquid storage chamber (541), a first connecting rod (58) is vertically and fixedly connected to the first sealing plate (57), the first connecting rod (58) penetrates through the upper end of the liquid storage seat (54) in a sliding manner, and a second matching wheel (59) is fixedly connected to the upper end of the first connecting rod (58);

The arc-shaped seat (510), the arc-shaped seat (510) is fixedly connected to the inner wall of one side, far away from the first opening (52), of the box body (51), the circle center of the arc-shaped seat (510) is located below the arc-shaped seat, and the second matching wheel (59) is used for being in contact with an arc-shaped surface (5101) of the arc-shaped seat (510);

the atomization nozzle (511), the atomization nozzle (511) is fixedly connected to one end of the liquid storage seat (54) located in the drying box (2), a liquid flow channel (512) is arranged in the liquid storage seat (54), and the liquid flow channel (512) is communicated with a liquid inlet of the atomization nozzle (511) and a liquid outlet of the liquid storage chamber (541);

the fixed end of the first horizontal electric telescopic rod (513) is fixedly connected to the inner wall of the lower end of the box body (51), and the telescopic end of the first horizontal electric telescopic rod (513) is fixedly connected with the first sliding block (517);

the fixing block (514), the fixing block (514) is fixedly connected to the inner wall of the lower end of the box body (51), one side, close to the first opening (52), of the fixing block (514) is provided with a matching inclined surface (5141), and the matching inclined surface (5141) is in contact with the first matching wheel (552);

the bracket six (515) is fixedly connected with the lower end of the first sliding block (517), the bracket six (515) penetrates through one end of the box body (51) in the drying box (2) in a sliding mode, and a temperature sensor is arranged on the bracket six (515);

The screening net (9) is vertically and slidably connected in the screening chamber (62), the left end of the screening net (9) penetrates through the left side of the screening chamber (62), a supporting block (91) is fixedly arranged on the inner side wall of the screening chamber (62), the screening net (9) is fixedly connected with a second spring at the lower end of the supporting block (91), and the crushing chamber (61) is communicated with the screening chamber (62) through a communication channel (12); the crushing device (8) comprises:

a crushing roller set (81), wherein the crushing roller set (81) is arranged in the crushing chamber (61) and is positioned at a feed inlet of the crushing and screening box (6);

the horizontal screw rod (82), the left and right sides of the horizontal screw rod (82) and the communication channel (12) are rotationally connected, the left and right parts of the part of the horizontal screw rod (82) positioned in the communication channel (12) are provided with screw thread sections with opposite rotation directions, the left end of the horizontal screw rod (82) penetrates through the left side of the communication channel (12), the left end of the horizontal screw rod (82) is fixedly connected with the output shaft of a second motor (83), and the second motor (83) is fixedly connected to the side wall of the crushing and screening box (6);

the two thread sliders (84), the two thread sliders (84) are respectively connected with the two thread sections in a threaded manner;

the left end of the left supporting plate (85) is rotationally connected with the left side of the lower end of the crushing chamber (61), and the right end of the right supporting plate (86) is rotationally connected with the right side of the lower end of the crushing chamber (61);

The lower ends of the two support rods (87) are respectively and rotatably connected with the upper ends of the two threaded sliding blocks (84), and the upper ends of the two support rods (87) are respectively and rotatably connected with the left support plate (85) and the right support plate (86);

the horizontal shaft (88) is rotationally connected with the left side wall of the crushing and screening box (6), a cam (89) is fixedly connected to the horizontal shaft (88), the cam (89) is in contact with the upper left part of the screening net (9), and the horizontal shaft (88) and the horizontal screw (82) are driven by a gear transmission group;

the upper end of the L-shaped guide rod (810) is fixedly connected with the upper end of the crushing and screening box (6), and the right end of the L-shaped guide rod (810) is fixedly connected with the right inner wall of the crushing and screening box (6);

the two second connecting rods (811) are sleeved on the horizontal part of the L-shaped guide rod (810) in a sliding manner at left and right intervals;

the grinding plates (812), the grinding plates (812) are fixedly connected to the lower ends of the two second connecting rods (811);

the vertical connecting rod (813) penetrates through the upper end of the crushing and screening box (6) in a sliding mode, a second opening (516) is formed in the upper end of the crushing and screening box (6), the vertical connecting rod (813) is connected in the second opening (516) in a sliding mode, and a second elastic sealing piece is connected between the vertical connecting rod (813) and the second opening (516);

The third horizontal electric telescopic rod (814), the stiff end fixed connection of third horizontal electric telescopic rod (814) smashes and screens case (6) upper end, the flexible end and the vertical connecting rod (813) upper end fixed connection of third horizontal electric telescopic rod (814).

2. The method for separating mogroside V from a waste liquid of fructus momordicae extraction according to claim 1, wherein in the step S2: concentrating to 1/2 of the original volume by vacuum;

3. The method for separating mogroside V from a waste liquid of fructus momordicae extraction as claimed in claim 2, wherein the vacuum concentration is-0.07 MPa to-0.088 MPa, and the concentration temperature is less than 60 ℃.

4. The method for separating mogroside V from waste liquid of fructus momordicae extraction as claimed in claim 2, wherein the step S3 is to remove impurities by high-speed centrifugation or flocculation precipitation, the step S7 is to dry by spray drying or freeze drying, and the steps of pulverizing, sieving, packaging and warehousing are sequentially performed after the drying.

5. The method for separating mogroside V from a waste liquid of Siraitia grosvenorii extraction of claim 4,

When in spray drying, the air inlet temperature is less than or equal to 180 ℃ and the air outlet temperature is 80-100 ℃;

and the vacuum degree is-0.00 MPa to-0.088 MPa during freeze drying.