CN106943447A - A kind of preparation method of Fructus Monordicae extract - Google Patents
A kind of preparation method of Fructus Monordicae extract Download PDFInfo
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- CN106943447A CN106943447A CN201710212466.1A CN201710212466A CN106943447A CN 106943447 A CN106943447 A CN 106943447A CN 201710212466 A CN201710212466 A CN 201710212466A CN 106943447 A CN106943447 A CN 106943447A
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- Prior art keywords
- momordica grosvenori
- extract
- resin
- fructus monordicae
- monordicae extract
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- 239000000284 extract Substances 0.000 title claims abstract description 41
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- 241001409321 Siraitia grosvenorii Species 0.000 claims abstract description 46
- 235000011171 Thladiantha grosvenorii Nutrition 0.000 claims abstract description 40
- 239000000463 material Substances 0.000 claims abstract description 32
- 238000000034 method Methods 0.000 claims abstract description 30
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 18
- 238000000605 extraction Methods 0.000 claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 14
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- 238000001694 spray drying Methods 0.000 claims abstract description 6
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- 238000000926 separation method Methods 0.000 claims description 18
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- TVJXHJAWHUMLLG-UHFFFAOYSA-N mogroside V Natural products CC(CCC(OC1OC(COC2OC(CO)C(O)C(O)C2OC3OC(CO)C(O)C(O)C3O)C(O)C(O)C1O)C(C)(C)O)C4CCC5(C)C6CC=C7C(CCC(OC8OC(COC9OC(CO)C(O)C(O)C9O)C(O)C(O)C8O)C7(C)C)C6(C)C(O)CC45C TVJXHJAWHUMLLG-UHFFFAOYSA-N 0.000 abstract description 10
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- 229930182470 glycoside Natural products 0.000 description 4
- 150000002338 glycosides Chemical class 0.000 description 4
- 239000003960 organic solvent Substances 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
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- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 2
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- 239000001397 quillaja saponaria molina bark Substances 0.000 description 2
- 229930182490 saponin Natural products 0.000 description 2
- 150000007949 saponins Chemical class 0.000 description 2
- 239000000741 silica gel Substances 0.000 description 2
- 229910002027 silica gel Inorganic materials 0.000 description 2
- 229960001866 silicon dioxide Drugs 0.000 description 2
- 210000000952 spleen Anatomy 0.000 description 2
- DKVBOUDTNWVDEP-NJCHZNEYSA-N teicoplanin aglycone Chemical compound N([C@H](C(N[C@@H](C1=CC(O)=CC(O)=C1C=1C(O)=CC=C2C=1)C(O)=O)=O)[C@H](O)C1=CC=C(C(=C1)Cl)OC=1C=C3C=C(C=1O)OC1=CC=C(C=C1Cl)C[C@H](C(=O)N1)NC([C@H](N)C=4C=C(O5)C(O)=CC=4)=O)C(=O)[C@@H]2NC(=O)[C@@H]3NC(=O)[C@@H]1C1=CC5=CC(O)=C1 DKVBOUDTNWVDEP-NJCHZNEYSA-N 0.000 description 2
- TWCMVXMQHSVIOJ-UHFFFAOYSA-N Aglycone of yadanzioside D Natural products COC(=O)C12OCC34C(CC5C(=CC(O)C(O)C5(C)C3C(O)C1O)C)OC(=O)C(OC(=O)C)C24 TWCMVXMQHSVIOJ-UHFFFAOYSA-N 0.000 description 1
- PLMKQQMDOMTZGG-UHFFFAOYSA-N Astrantiagenin E-methylester Natural products CC12CCC(O)C(C)(CO)C1CCC1(C)C2CC=C2C3CC(C)(C)CCC3(C(=O)OC)CCC21C PLMKQQMDOMTZGG-UHFFFAOYSA-N 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
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- 239000000470 constituent Substances 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- ZYZJWAJOTPNVPI-ZVBSCDOUSA-N cucurbitane Chemical compound C([C@H]1[C@]2(C)CC[C@@H]([C@]2(CC[C@]11C)C)[C@H](C)CCCC(C)C)CC2[C@H]1CCCC2(C)C ZYZJWAJOTPNVPI-ZVBSCDOUSA-N 0.000 description 1
- 230000035622 drinking Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000003599 food sweetener Nutrition 0.000 description 1
- 235000021022 fresh fruits Nutrition 0.000 description 1
- 229940068517 fruit extracts Drugs 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 125000003147 glycosyl group Chemical group 0.000 description 1
- 235000008216 herbs Nutrition 0.000 description 1
- PFOARMALXZGCHY-UHFFFAOYSA-N homoegonol Natural products C1=C(OC)C(OC)=CC=C1C1=CC2=CC(CCCO)=CC(OC)=C2O1 PFOARMALXZGCHY-UHFFFAOYSA-N 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 239000004005 microsphere Substances 0.000 description 1
- 239000002798 polar solvent Substances 0.000 description 1
- 238000004886 process control Methods 0.000 description 1
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- 239000000377 silicon dioxide Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000003765 sweetening agent Substances 0.000 description 1
- 230000035922 thirst Effects 0.000 description 1
- 150000003648 triterpenes Chemical group 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/42—Cucurbitaceae (Cucumber family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation or decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a kind of method that Continuous Countercurrent Extraction prepares Fructus Monordicae extract, this method is inputted after directly Momordica grosvenori is crushed and extracted in Continuous Countercurrent Extraction equipment, then by filtering and impurity removing, except color, absorption exquisiteness, concentrated spray drying, finally give Fructus Monordicae extract, wherein Mogroside V content >=40% in Fructus Monordicae extract.The preparation method that the present invention is provided, whole process is simply used as Hydrolysis kinetics solvent, safety non-toxic with pure water or hydrous ethanol.Method used herein is simplified, can continuous operation, saved a large amount of manpower and materials, required Preparation equipment and site requirements is low, be adapted to industrialized production.
Description
Technical field
Separation preparation field is extracted the invention belongs to active ingredient of natural plant, is related to a kind of preparation of Fructus Monordicae extract
Method, more particularly to a kind of Mogroside V content are more than the preparation method of 40% Fructus Monordicae extract.
Background technology
Momordica grosvenori is the fruit that Momordica grosvenori belongs to cucurbitaceous plant, is a kind of multipurpose integration of drinking and medicinal herbs medicinal material.Arhat fruity pole
It is sweet, cool in nature, nontoxic, enter spleen and lung warp, with removing heat from the lung to relieve cough, relax bowel, clearing heat and detoxicating, beneficial liver spleen, calmness are quenched the thirst, promote stomach
A variety of pharmacological effects such as enteron aisle function, antiviral, antitumor, hypoglycemic step-down.Numerous studies data shows that Momordica grosvenori not only contains
A variety of pharmacological components, and can be used as the sweetener of low sugar contents, therefore it is widely used in food, health care at present
Product, medicine, beverage, cosmetic industry.
Contain a variety of saponin(e active components in Momordica grosvenori, wherein momordica grosvenori glycoside V is one of leading indicator composition, arhat
Fruit saponin(e aglycon has typical cucurbitane type tetracyclic triterpene structure.Momordica grosvenori glycoside V can be used as evaluation Fructus Monordicae extract matter
Measure good and bad index composition because its aglycon combines glycosyl and soluble in water, ethanol polar solvent, be insoluble in acetone,
The organic solvents such as ethyl acetate, end-product is white amorphous powder.
The research prepared on Fructus Monordicae extract is concentrated mainly on the country, and Patents documents report is more, but really
The less of production can be applied to, the following aspects is mainly reflected in：（1）Process is cumbersome, and yield is low；（2）Using a large amount of
Organic solvent, high to equipment requirement, input is big；（3）Technical process energy consumption is big, high cost；（4）Other materials are introduced in process,
Influence product quality.As CN101029071B discloses the method that high-purity Momordia grosvenori aglycone V is prepared from Momordica grosvenori, this method
It is that Momordica grosvenori dry fruit is subjected to alcohol extracting, concentration, through ethyl acetate, extracting n-butyl alcohol, then using silicagel column, C18 reverse phase silica gels
The sweet glycosides V products of high-purity are obtained after column chromatography, drying, this method major defect is technique is cumbersome, solvent loss is big, yield is low, no
Beneficial to scale industrial production；CN100572552C discloses Fructus Monordicae extract and its preparation of momordica grosvenori glycoside V >=40%
Method, this method is, by the water extraction of Momordica grosvenori fresh fruit, concentration, enzymolysis, macroporous resin adsorption, ion-exchange resin decolorization, most to pass through afterwards
Concentrate drying obtains product, and this method causes the subsequent treatment cost of resin larger, be unfavorable for due to not filtered before upper prop
Continuous production；CN101407535B discloses a kind of preparation method of high-purity Momordica grosvenori mogroside V, and this method is to use one
The Fructus Monordicae extract for determining purity is raw material, is dissolved through methanol, acetone extract, purification on normal-phase silica gel are adsorbed, separated, crystallized and recrystallization
Obtain final products, this method technique is simple, but during used substantial amounts of organic solvent, the requirement to Workplace compared with
Height, the product obtained in addition also has certain organic solvent residual, influences quality；CN101863946B discloses a kind of dry sieve
The method that Chinese fruit extracts high content Momordica grosvenori mogroside V, this method be by Momordica grosvenori dry fruit it is broken after, through alcohol extracting, concentration, centrifugation,
Macroporous resin adsorption separation, ion-exchange resin decolorization, last concentrated, drying, obtain finished product, the method is due to filtered place
Reason so that big molecular impurity is more in upper prop liquid, influences the absorption property of resin, utilization rate is low and subsequent treatment difficulty relatively
Greatly, product cost is higher.
The content of the invention
It is an object of the invention to provide a kind of Fructus Monordicae extract preparation method suitable for industrialized production, this method
The defect of above-mentioned existing technology of preparing is can solve the problem that, being capable of continuous production and with low cost, low energy consumption, high income, safety
Efficiently the features such as.
A kind of preparation method of Fructus Monordicae extract, comprises the following steps：
（1）Medicinal material pre-treatment：Momordica grosvenori is suitably crushed, the material after crushing is not broken for degree with arhat fruit seed；
（2）Continuous Countercurrent Extraction：By in the Momordica grosvenori input continuous flow upstream tubular type extractor after break process, inversely input pure
Water, its reclaimed water is 5-8 with quality of medicinal material ratio:1, and it is 45-85 DEG C to keep Continuous Countercurrent Extraction pipe temperature, extracts 30-60min,
Obtain Momordica grosvenori extract solution；
（3）Centrifugal filtration：Obtained Momordica grosvenori extract solution is inputted into liquid-slag separation device, expression separation is carried out by 40-100kg/h
Bulky grain material residue is removed, high speed centrifugation device is then inputted, with 2000-6000r/min centrifugation 20-60min, obtains sieve
Chinese berry extract centrifugate；
（4）Except color：Centrifugate is imported into strong alkalinity anion macropore decolorizing resin, being eluted to efflux without sweet taste with purified water is
Only, Fructus Monordicae extract destainer is obtained, wherein decolorizing resin consumption is 0.01-0.1 times of centrifugate volume, it is therefore preferable to
0.05-0.8 times；
（5）Fluoropolymer resin separation and purification：Destainer is imported into fluoropolymer resin adsorption, 20%-80% ethanol waters are used
Untill efflux is eluted to without sweet taste, final Momordica grosvenori pregnant solution is obtained, wherein fluoropolymer resin consumption is the 0.02- of destainer
0.08 times, it is therefore preferable to 0.04-0.06 times；
（6）Concentrated spray is dried：Density will be concentrated to below 85 DEG C of pregnant solution vacuum decompression for 1.12-1.25g/ml, Ran Houzai
Spray drying obtains final finished, and finished product is white amorphous powder or off-white color material, wherein Mogroside V content >=
40%.This finished product can further be refining to obtain the higher Fructus Monordicae extract of Mogroside V content.
Further, step（4）Described in decolorizing resin from D151, D303, S-8, D-309, LX-700, D290 or
XDA-7 resins, wherein it is preferred that D303, D290 or LX-700 resin；The decolorizing resin post blade diameter length ratio is 1:2-1:10, its
In be preferably 1:3-1:5.
Further, step（5）Described in fluoropolymer resin refer to styrene, methacrylate or styrene and first
Base acrylate copolymer be matrix polymer filler, preferred substrate be styrene and methacrylate copolymer, it is selected
Packing material size is 5 μm-300 μm, it is therefore preferable to 50 μm-150 μm, and selected filler aperture is 100-1000, it is therefore preferable to
300-500Å。
Advantage of the present invention：
1. the present invention will directly take the mode of Continuous Countercurrent Extraction to be extracted after Momordica grosvenori break process, letter, which is saved, significantly extracts
Step, and can continuously produce, one side sample introduction is while slag tap.The whole pure water or aqueous harmless using low toxicity in preparation process
Ethanol produces highly effective and safe as Hydrolysis kinetics solvent, and the requirement for production site, equipment is low, is adapted to industrialization extensive
Production.
2. the present invention use strong alkalinity anion macroreticular resin carry out preliminary decolouring and removal of impurities handle after be introduced directly into
Styrene and methacrylate copolymer carry out further separation exquisiteness, final concentrated spray for the microspheric filler of matrix
End-product is dried to obtain, whole process is carried out continuously, resin regenerating easily recycling used greatly improves preparation effect
Rate, saves the time, simplifies process, be conducive to process control and quality control, be especially suitable for industrialized production.
3. end-product active constituent content is high, impurity content is low, Mogroside V content in gained Fructus Monordicae extract >=
40%。
Embodiment
Following examples are intended to further illustrate the present invention, rather than limitation of the present invention, and all are based on institute of the present invention
The improvement made is all within the scope of the present invention.
Embodiment 1
（1）Medicinal material pre-treatment：Momordica grosvenori 1000kg is suitably crushed, the material after crushing is not broken for degree with arhat fruit seed；
（2）Continuous Countercurrent Extraction：Momordica grosvenori after break process is inputted in continuous flow upstream tubular type extractor, by solvent medicinal material matter
Amount compares 8:1 inversely inputs the pure water of 8 times of quality of medicinal materials, and it is 65 DEG C to keep Continuous Countercurrent Extraction pipe temperature, is extracted
45min, obtains Momordica grosvenori extract solution；
（3）Centrifugal filtration：Obtained Momordica grosvenori extract solution is inputted into liquid-slag separation device, expression separation removing is carried out by 80kg/h
Bulky grain material residue, then input high speed centrifugation device, with 4500r/min centrifugation 30min, obtain Fructus Monordicae extract from
Heart liquid；
（4）Except color：Centrifugate is imported into D303 decolorizing resins, untill being eluted to efflux without sweet taste with purified water, arhat is obtained
Berry extract destainer, wherein decolorizing resin consumption are 0.03 times of centrifugate volume；
（5）Fluoropolymer resin separation and purification：Adsorption during destainer is imported using styrene as the polymer filler of matrix, is used
Untill 50% edible ethanol is eluted to efflux without sweet taste, final Momordica grosvenori pregnant solution, wherein styrenic polymer resins are obtained
Consumption is 0.04 times of destainer；
（6）Concentrated spray is dried：Density will be concentrated to below 85 DEG C of pregnant solution vacuum decompression for 1.12-1.25g/ml, Ran Houzai
Spray drying obtains final finished, and finished product is white amorphous powder or off-white color material, and wherein Mogroside V content is
45.23%。
Embodiment 2
（1）Medicinal material pre-treatment：500kg Momordica grosvenoris are suitably crushed, the material after crushing is not broken for degree with arhat fruit seed；
（2）Continuous Countercurrent Extraction：Momordica grosvenori after break process is inputted in continuous flow upstream tubular type extractor, with solvent medicinal material matter
Amount compares 6:1 ratio inversely inputs the pure water of 6 times of quality of medicinal materials, and it is 55 DEG C to keep Continuous Countercurrent Extraction pipe temperature, is carried
30min is taken, Momordica grosvenori extract solution is obtained；
（3）Centrifugal filtration：Obtained Momordica grosvenori extract solution is inputted into liquid-slag separation device, expression separation removing is carried out by 60kg/h
Bulky grain material residue, then input high speed centrifugation device, with 2000r/min centrifugation 55min, obtain Fructus Monordicae extract from
Heart liquid；
（4）Except color：Centrifugate is imported into D290 decolorizing resins, untill being eluted to efflux without sweet taste with purified water, arhat is obtained
Berry extract destainer, wherein decolorizing resin consumption are 0.05 times of centrifugate volume；
（5）Fluoropolymer resin separation and purification：It is enriched with during destainer is imported using methacrylate as the polymer filler of matrix
It is refined, untill being eluted to efflux without sweet taste with 35% edible ethanol, final Momordica grosvenori pregnant solution is obtained, wherein styrene gathers
Polymer resin consumption is 0.06 times of destainer；
（6）Concentrated spray is dried：Density will be concentrated to below 85 DEG C of pregnant solution vacuum decompression for 1.12-1.25g/ml, Ran Houzai
Spray drying obtains final finished, and finished product is white amorphous powder or off-white color material, and wherein Mogroside V content is
49.56%。
Embodiment 3
（1）Medicinal material pre-treatment：Momordica grosvenori 800kg is suitably crushed, the material after crushing is not broken for degree with arhat fruit seed；
（2）Continuous Countercurrent Extraction：Momordica grosvenori after break process is inputted in continuous flow upstream tubular type extractor, with solvent medicinal material matter
Amount compares 6:1 reverse input purified water, and it is 45 DEG C to keep Continuous Countercurrent Extraction pipe temperature, extracts 30min, obtains Momordica grosvenori extraction
Liquid；
（3）Centrifugal filtration：Obtained Momordica grosvenori extract solution is inputted into liquid-slag separation device, expression separation removing is carried out by 88kg/h
Bulky grain material residue, then input high speed centrifugation device, with 6000r/min centrifugation 30min, obtain Fructus Monordicae extract from
Heart liquid；
（4）Except color：Centrifugate is imported into LX-700 decolorizing resins, untill being eluted to efflux without sweet taste with purified water, sieve is obtained
Chinese berry extract destainer, wherein decolorizing resin consumption are 0.05 times of centrifugate volume；
（5）Fluoropolymer resin separation and purification：Destainer is imported using styrene and methacrylate copolymer as the poly- of matrix
Adsorption in compound filler, untill being eluted to efflux without sweet taste with 65% edible ethanol, obtains final Momordica grosvenori enrichment
Liquid, wherein styrenic polymer resins consumption are 0.06 times of destainer；
（6）Concentrated spray is dried：Density will be concentrated to below 85 DEG C of pregnant solution vacuum decompression for 1.12-1.25g/ml, Ran Houzai
Spray drying obtains final finished, and finished product is white amorphous powder or off-white color material, and wherein Mogroside V content is
42.38%。
Claims (5)
1. a kind of preparation method of Fructus Monordicae extract, it is characterised in that the preparation method of the extract comprises the following steps：
（1）Medicinal material pre-treatment：Momordica grosvenori is suitably crushed, the material after crushing is not broken for degree with arhat fruit seed；
（2）Continuous Countercurrent Extraction：By in the Momordica grosvenori input continuous flow upstream tubular type extractor after break process, reverse input is purified
Water, wherein solvent quality of medicinal material ratio are 5-8:1, and it is 45-85 DEG C to keep Continuous Countercurrent Extraction pipe temperature, extracts 30-60min,
Obtain Momordica grosvenori extract solution；
（3）Centrifugal filtration：Obtained Momordica grosvenori extract solution is inputted into liquid-slag separation device, expression separation is carried out by 40-100kg/h
Bulky grain material residue is removed, high speed centrifugation device is then inputted, with 2000-6000r/min centrifugation 20-60min, obtains sieve
Chinese berry extract centrifugate；
（4）Except color：Centrifugate is imported into strong alkalinity anion macropore decolorizing resin, being eluted to efflux without sweet taste with purified water is
Only, Fructus Monordicae extract destainer is obtained；
（5）Fluoropolymer resin separation and purification：Destainer is imported into fluoropolymer resin adsorption, it is water-soluble with 20%-80% ethanol
Untill liquid is eluted to efflux without sweet taste, final Momordica grosvenori pregnant solution is obtained；
（6）Concentrated spray is dried：Density will be concentrated to below 85 DEG C of pregnant solution vacuum decompression for 1.12-1.25g/ml, Ran Houzai
Spray drying obtains final finished, and finished product is white amorphous powder or off-white color material.
2. such as step in claim 1（4）The decolorizing resin, its consumption is 0.01-0.1 times of centrifugate volume, preferably
For 0.05-0.8 times.
3. such as step in claim 1（4）Described in decolorizing resin, can from D151, D303, S-8, D-309, LX-700,
D290 or XDA-7 resins, wherein it is preferred that D303, D290 or LX-700 resin；The decolorizing resin post blade diameter length ratio is 1:2-
1:10, wherein preferably 1:3-1:5.
4. such as step in claim 1（5）Described fluoropolymer resin, its consumption is 0.02-0.08 times of destainer, preferably
For 0.04-0.06 times.
5. such as step in claim 1（5）Described in fluoropolymer resin, refer to styrene, methacrylate or styrene
With methacrylate copolymer be matrix polymer filler, preferred substrate be styrene and methacrylate copolymer,
Selected packing material size is 5 μm-300 μm, it is therefore preferable to 50 μm-150 μm, and selected filler aperture is 100-1000, preferably
Ground is 300-500.
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