CN102382205A - Method for extracting pectin from banana peels - Google Patents
Method for extracting pectin from banana peels Download PDFInfo
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- CN102382205A CN102382205A CN 201110309749 CN201110309749A CN102382205A CN 102382205 A CN102382205 A CN 102382205A CN 201110309749 CN201110309749 CN 201110309749 CN 201110309749 A CN201110309749 A CN 201110309749A CN 102382205 A CN102382205 A CN 102382205A
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Abstract
The invention relates to a method for extracting pectin from banana peels, comprising the following steps of: (1) acid regulation of raw material: cleaning and drying the banana peels, smashing and slagging for use and preparing a material liquid in the weight proportion of material to liquid 1: (5-30); (2) surfactant solubilization: adding a surfactant into the material liquid subject to the acid regulation and stirring; (3) ultrasonic treatment: carrying out pectin extraction on the material liquid subject to the surfactant treatment by utilizing ultrasonic radiation; (4) enzyme treatment: adding enzyme into the material liquid subject to the ultrasonic treatment so as to carry out enzymatic extraction; (5) alcohol precipitation: carrying out filtering and deslagging on the material liquid subject to the enzyme treatment, adding alcohol into the obtained filtrate and precipitating; and (6) drying: putting precipitate in a constant-temperature drying oven, drying, smashing and obtaining pectin end products. The method provided by the invention has the advantages of high extracted pectin yield, obvious decolorizing effect, short extracting time, high pectin purity and stable activity, therefore, a mass of resources can be saved and obvious economic benefit is provided.
Description
Technical field
The present invention relates to a kind of method of from Pericarpium Musae, extracting pectin.
Background technology
Can suppress the absorption of enteron aisle with the aluminium salt of pectin, can be used as the assisting therapy of cardiovascular disorder SUV and triglyceride.
Pectin mainly extracts from plant tissue at present, and common method has acid system, salting-out process, enzyme process, microwave-assisted and ultrasonic auxiliary law.The land bud spring, (patent No.: mention the method for extracting pectin in the Pericarpium Musae with microwave-assisted 200710168106.2), though this method can improve the yield of pectin, percent of decolourization was lower, and destroys the physiologically active of pectin easily in its patent; Liu Minyao its patent (patent No.: mention 200610013391.6) with zymin to apple residue in starchy material carry out the method that enzymolysis is produced apple pectin, though this method technology is simple, improve the purity of pectin, the yield of pectin is lower; The method of pectin during Li Haoxian mentions with the ultrasonic-assisted extraction pineapple peel in its patent (200910040946.x); Though this method can improve the yield of pectin; Shorten extraction time; But because its extraction temperature is higher, percent of decolourization is relatively poor, and destroys the purity and the physiologically active of pectin easily.
Summary of the invention
The purpose of this invention is to provide a kind of new method of from Pericarpium Musae, extracting pectin.
The present invention includes following steps:
(1) raw material acid adjustment: Pericarpium Musae cleaned dry, pulverize slagging and use, itself and water are mixed with feed liquid by the weight ratio than 1:5 ~ 30;
(2) show the promoting agent solubilising: stir after in the feed liquid that mixes up acid, adding tensio-active agent;
(3) supersound process: process is shown that the feed liquid that promoting agent was handled adopts ultrasonic radiation to carry out the pectin extraction;
(4) enzyme is handled: carrying out the enzymatic extraction through adding enzyme in the feed liquid of supersound process;
(5) alcohol is analysed: the feed liquid to after handling through enzyme is carried out filter cleaner, and gained filtrating adds ethanol and precipitates;
(6) drying: throw out is put in carries out drying in the thermostatic drying chamber, promptly make the pectin finished product through pulverizing again.
Be to improve the dissolution rate of Pericarpium Musae, and then improve the extraction yield of pectin, above-mentioned steps 1) in Pericarpium Musae cleaned be ground into 100 ~ 200 purpose slags after drying.For reducing impurity as far as possible
,Water preferably adopts aquae destillata.
Because tensio-active agent can reduce the IT of solid-liquid phase, ability is oozed out in the dissolving of increase larger molecular organics matter, therefore in leaching process, can shorten infiltrating time, improve extraction rate.So the present invention is in step 2) in adopt tensio-active agent to carry out solubilising, aforementioned surfactants is selected from polyoxyethylenated alcohol sodium sulfate (AES), tween 80 (span-80), AEO (AEO
9) or sodium laurylsulfonate (SDS), its addition is 0.5 ~ 1% of a feed liquid weight
,Churning time behind the adding tensio-active agent is 10 ~ 20 minutes.
Above-mentioned steps 3) in the said supersound process process, ultrasonic power is 400 ~ 600W, and the treatment time is 10 ~ 15 minutes.
Above-mentioned steps 4) described enzyme preferably selects polygalacturonase, neutral protease, cellulase, and its addition is for through 0.05 ~ 0.1% of the feed liquid weight of supersound process.Through after adding enzyme in the feed liquid of supersound process, feed liquid heated is incubated 8 ~ 15 minutes after 30-60 ℃.
Above-mentioned steps 5) used alcoholic acid weight concentration is 95% in, and its consumption is at least a times through the material liquid volume after the enzyme processing.
Above-mentioned steps 6) drying temperature in is 45 ~ 60 ℃, and being dried to like water cut is below 5%.
Advantage of the present invention is:
(1) percent of decolourization high (can reach more than 76%); The pectin Stability Analysis of Structures do not influence the physiologically active of pectin, and purity is higher: because in whole extraction process; Do not relate to too high temperature; Coloring matter is difficult for stripping in the Pericarpium Musae, and tensio-active agent, supersound process, enzymically treat have specific single-minded functionality, belongs to soft extraction.
(2) the pectin extraction yield saves time up to about 20%: the present invention can make Pericarpium Musae pectin extracted fully, and be effect in 30 minutes action time (whole abstraction reaction time).
(3) environmental protection: the pollution that has overcome traditional method in the whole extraction process is big, the phenomenon of serious waste of resources.
Embodiment
Embodiment 1
(1) Pericarpium Musae cleaned dried, be crushed to 100 orders, itself and zero(ppm) water are mixed with feed liquid by the weight ratio than 1:20 after, using the pH value of flavouring liquid is 1.8;
(2) in the feed liquid that mixes up acid, stir behind the adding tensio-active agent sodium laurylsulfonate (SDS), the addition of sodium laurylsulfonate (SDS) is 0.8% of a feed liquid weight, and the churning time after the adding is 15 minutes;
(3) process is shown that the feed liquid that promoting agent was handled adopts ultrasonic radiation to carry out the pectin extraction, ultrasonic power 500W, the extraction time is 12 minutes;
(4) carrying out the enzymatic extraction in 10 minutes through insulation after adding cellulase in the feed liquid of supersound process and heating to 45 ℃, the addition of cellulase is 0.06% of a feed liquid weight;
(5) feed liquid after handling through enzyme is carried out filter cleaner, gained isopyknic adding weight concentration of filtrating is that 95% ethanol precipitates;
(6) throw out is put under 45 ~ 60 ℃ condition, carries out drying in the thermostatic drying chamber, promptly make the pectin finished product through pulverizing again.
The extraction yield of present embodiment pectin is 20.26%.
Embodiment 2
(1) Pericarpium Musae cleaned dried, be crushed to 100 orders, itself and zero(ppm) water are mixed with feed liquid by the weight ratio than 1:15 after, using the pH value of flavouring liquid is 1.6;
(2) in the feed liquid that mixes up acid, stir behind the adding tensio-active agent polyoxyethylenated alcohol sodium sulfate (AES), the addition of polyoxyethylenated alcohol sodium sulfate (AES) is 1% of a feed liquid weight, and the churning time after the adding is 15 minutes;
(3) process is shown that the feed liquid that promoting agent was handled adopts ultrasonic radiation to carry out the pectin extraction, ultrasonic power 500W, the extraction time is 12 minutes;
(4) carrying out the enzymatic extraction in 8 minutes through insulation after adding polygalacturonase in the feed liquid of supersound process and heating to 50 ℃, the addition of cellulase is 0.08% of a feed liquid weight;
(5) feed liquid after handling through enzyme is carried out filter cleaner, gained isopyknic adding weight concentration of filtrating is that 95% ethanol precipitates;
(6) throw out is put under 45 ~ 60 ℃ condition, carries out drying in the thermostatic drying chamber, promptly make the pectin finished product through pulverizing again.
The extraction yield of present embodiment pectin is 19.8%.
Embodiment 3
(1) Pericarpium Musae cleaned dried, be crushed to 200 orders, itself and zero(ppm) water are mixed with feed liquid by the weight ratio than 1:10 after, using the pH value of flavouring liquid is 1.8;
(2) in the feed liquid that mixes up acid, stir behind the adding tensio-active agent tween 80 (span-80), the addition of tween 80 (span-80) is 0.7% of a feed liquid weight, and the churning time after the adding is 20 minutes;
(3) process is shown that the feed liquid that promoting agent was handled adopts ultrasonic radiation to carry out the pectin extraction, ultrasonic power 500W, the extraction time is 15 minutes;
(4) carrying out the enzymatic extraction in 10 minutes through insulation after adding cellulase in the feed liquid of supersound process and heating to 55 ℃, the addition of cellulase is 0.08% of a feed liquid weight;
(5) feed liquid after handling through enzyme is carried out filter cleaner, gained isopyknic adding weight concentration of filtrating is that 95% ethanol precipitates;
(6) throw out is put under 45 ~ 60 ℃ condition, carries out drying in the thermostatic drying chamber, promptly make the pectin finished product through pulverizing again.
The extraction yield of present embodiment pectin can reach 19.65%.
Embodiment 4
(1) Pericarpium Musae cleaned dried, be crushed to 200 orders, itself and zero(ppm) water are mixed with feed liquid by the weight ratio than 1:25 after, using the pH value of flavouring liquid is 1.6;
(2) in the feed liquid that mixes up acid, add tensio-active agent AEO (AEO
9) after stir AEO (AEO
9) addition be 0.8% of feed liquid weight, the churning time after the adding is 20 minutes;
(3) process is shown that the feed liquid that promoting agent was handled adopts ultrasonic radiation to carry out the pectin extraction, ultrasonic power 400W, the extraction time is 15 minutes;
(4) carrying out the enzymatic extraction in 15 minutes through insulation after adding neutral protease in the feed liquid of supersound process and heating to 45 ℃, the addition of cellulase is 0.05% of a feed liquid weight;
(5) feed liquid after handling through enzyme is carried out filter cleaner, gained isopyknic adding weight concentration of filtrating is that 95% ethanol precipitates;
(6) throw out is put under 45 ~ 60 ℃ condition, carries out drying in the thermostatic drying chamber, promptly make the pectin finished product through pulverizing again.
The extraction yield of present embodiment pectin can reach 19.34%.
Claims (10)
1. a method of extracting Pericarpium Musae pectin is characterized in that comprising the steps:
(1) raw material acid adjustment: Pericarpium Musae cleaned dries, pulverize slagging and use, itself and water are mixed with feed liquid by the weight ratio than 1:5 ~ 30 after, using the pH value of flavouring liquid is 1.5 ~ 1.8;
(2) show the promoting agent solubilising: stir after in the feed liquid that mixes up acid, adding tensio-active agent;
(3) supersound process: process is shown that the feed liquid that promoting agent was handled adopts ultrasonic radiation to carry out the pectin extraction;
(4) enzyme is handled: carrying out the enzymatic extraction through adding enzyme in the feed liquid of supersound process;
(5) alcohol is analysed: the feed liquid to after handling through enzyme is carried out filter cleaner, and gained filtrating adds ethanol and precipitates;
(6) drying: throw out is put in carries out drying in the thermostatic drying chamber, promptly make the pectin finished product through pulverizing again.
2. method according to claim 1 is characterized in that in the step 1) adopting the pH value of hydrochloric acid flavouring liquid.
3. method according to claim 1 and 2 is characterized in that in the step 1) Pericarpium Musae cleaned and is ground into 100 ~ 200 purpose slags after drying.
4. method according to claim 1 is characterized in that step 2) said tensio-active agent is selected from polyoxyethylenated alcohol sodium sulfate (AES), tween 80 (span-80), AEO (AEO
9) or sodium laurylsulfonate (SDS).
5. according to claim 1 or 4 described methods, it is characterized in that step 2) in aforementionedly show that the addition of promoting agent is 0.5 ~ 1% of a feed liquid weight
,Churning time behind the adding tensio-active agent is 10 ~ 20 minutes.
6. method according to claim 1 is characterized in that in the step 3) supersound process process, ultrasonic power is 400 ~ 600W, and the treatment time is 10 ~ 15 minutes.
7. method according to claim 1 is characterized in that the described enzyme of step 4) is selected from polygalacturonase, neutral protease, cellulase, and its addition is through 0.05 ~ 0.1% of the feed liquid weight of supersound process.
8. according to claim 1 or 5 described methods, it is characterized in that in the step 4) that through after adding enzyme in the feed liquid of supersound process feed liquid is heated is incubated 8 ~ 15 minutes after 30-60 ℃.
9. method according to claim 1 is characterized in that the alcoholic acid weight concentration 95% that step 5) is used, and its consumption is at least a times through the material liquid volume after the enzyme processing.
10. method according to claim 1 is characterized in that the drying temperature in the step 6) is 45 ~ 60 ℃, and being dried to water cut is below 5%.
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103073657A (en) * | 2013-01-15 | 2013-05-01 | 中北大学 | Method for manufacturing sunflower low-fat pectins |
| CN103641930A (en) * | 2013-11-27 | 2014-03-19 | 威海市桢昊生物技术有限公司 | Method for extraction of pectin from pineapple peels and residue by enzymatic process |
| CN103767969A (en) * | 2014-01-14 | 2014-05-07 | 上海海洋大学 | Hand cream prepared from effective components in banana peels and preparation method of hand cream |
| CN103947968A (en) * | 2014-04-30 | 2014-07-30 | 桂林军供生化技术开发有限公司 | Banana extract, preparation method and application of banana extract |
| CN104725519A (en) * | 2015-03-09 | 2015-06-24 | 梁永锋 | Method for extracting and purifying banana peel polysaccharide |
| CN105237653A (en) * | 2015-11-13 | 2016-01-13 | 谢镜国 | Longan pectin extraction method |
| CN105481995A (en) * | 2016-01-28 | 2016-04-13 | 蒋丽红 | Method for extracting banana peel pectin |
| CN107771185A (en) * | 2015-06-17 | 2018-03-06 | 杜邦营养生物科学有限公司 | method for extracting pectin |
| CN108402252A (en) * | 2018-05-17 | 2018-08-17 | 大连民族大学 | A kind of Schisandra chinensis multiple elements design tea beverage |
| CN106008735B (en) * | 2016-06-27 | 2019-04-30 | 西北农林科技大学 | A kind of method that ultrasonic synergistic surfactant extracts polysaccharides |
| CN110527703A (en) * | 2019-07-12 | 2019-12-03 | 浙江工业大学 | A kind of sunflower pectin oligosaccharide extract and the preparation method and application thereof |
| CN111500656A (en) * | 2020-04-14 | 2020-08-07 | 广西农业职业技术学院 | Method for extracting low-molecular-weight pectin from banana peel |
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| CN101148482A (en) * | 2007-11-05 | 2008-03-26 | 桂林工学院 | Method for extracting pectin from banana peel |
| CN101160327A (en) * | 2005-03-09 | 2008-04-09 | 卡吉尔股份有限公司 | Process for obtaining pectin |
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| CN101160327A (en) * | 2005-03-09 | 2008-04-09 | 卡吉尔股份有限公司 | Process for obtaining pectin |
| CN101148482A (en) * | 2007-11-05 | 2008-03-26 | 桂林工学院 | Method for extracting pectin from banana peel |
Non-Patent Citations (1)
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| 《农业机械》 20110630 岳贤田 表面活性剂增效超声波辅助提取香蕉皮中果胶的研究 145-147 1-10 , 第17期 * |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103073657B (en) * | 2013-01-15 | 2015-08-19 | 中北大学 | A kind of Sunflower Receptacle low-fat pectin production method |
| CN103073657A (en) * | 2013-01-15 | 2013-05-01 | 中北大学 | Method for manufacturing sunflower low-fat pectins |
| CN103641930A (en) * | 2013-11-27 | 2014-03-19 | 威海市桢昊生物技术有限公司 | Method for extraction of pectin from pineapple peels and residue by enzymatic process |
| CN103767969B (en) * | 2014-01-14 | 2016-06-01 | 上海海洋大学 | A kind of hand lotion obtained by effective constituent in Pericarpium Musae and its preparation method |
| CN103767969A (en) * | 2014-01-14 | 2014-05-07 | 上海海洋大学 | Hand cream prepared from effective components in banana peels and preparation method of hand cream |
| CN103947968A (en) * | 2014-04-30 | 2014-07-30 | 桂林军供生化技术开发有限公司 | Banana extract, preparation method and application of banana extract |
| CN104725519A (en) * | 2015-03-09 | 2015-06-24 | 梁永锋 | Method for extracting and purifying banana peel polysaccharide |
| CN107771185A (en) * | 2015-06-17 | 2018-03-06 | 杜邦营养生物科学有限公司 | method for extracting pectin |
| CN105237653A (en) * | 2015-11-13 | 2016-01-13 | 谢镜国 | Longan pectin extraction method |
| CN105481995A (en) * | 2016-01-28 | 2016-04-13 | 蒋丽红 | Method for extracting banana peel pectin |
| CN106008735B (en) * | 2016-06-27 | 2019-04-30 | 西北农林科技大学 | A kind of method that ultrasonic synergistic surfactant extracts polysaccharides |
| CN108402252A (en) * | 2018-05-17 | 2018-08-17 | 大连民族大学 | A kind of Schisandra chinensis multiple elements design tea beverage |
| CN110527703A (en) * | 2019-07-12 | 2019-12-03 | 浙江工业大学 | A kind of sunflower pectin oligosaccharide extract and the preparation method and application thereof |
| CN111500656A (en) * | 2020-04-14 | 2020-08-07 | 广西农业职业技术学院 | Method for extracting low-molecular-weight pectin from banana peel |
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Application publication date: 20120321 |