CN104725519A - Method for extracting and purifying banana peel polysaccharide - Google Patents
Method for extracting and purifying banana peel polysaccharide Download PDFInfo
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- CN104725519A CN104725519A CN201510101049.0A CN201510101049A CN104725519A CN 104725519 A CN104725519 A CN 104725519A CN 201510101049 A CN201510101049 A CN 201510101049A CN 104725519 A CN104725519 A CN 104725519A
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Abstract
The invention relates to the technical field of extraction and particularly relates to a method for extracting and purifying banana peel polysaccharide. The method comprises the following steps: adding purified water into a banana peel sample; shaking uniformly; soaking for 30 to 90 minutes at the temperature of 80 DEG C; ultrasonically extracting for 30 to 60 minutes at 60W to 100W; centrifuging; taking a liquid supernatant; repeatedly extracting filter residues; combining the liquid supernatants; decompressing and concentrating; adding 5wt% trichloroacetic acid into a concentrated solution for extraction; vibrating and centrifuging; taking the liquid supernatant; repeating the extraction operation; combining the liquid supernatants; adding 0.3 time volume of 10wt% H2O2; vibrating and standing; filtering; taking filtrate and repeating the operations to obtain banana peel filtrate; adding 0.3 times volume of 95wt% ethanol; precipitating at the temperature of 4 DEG C; centrifuging for 10min at a speed of 9000r/min; taking the precipitate to obtain the banana peel polysaccharide. By extracting the banana peel polysaccharide in an ultrasonic manner, the method disclosed by the invention is high in extraction rate, simple and convenient to operate and good in safety.
Description
Technical field
The present invention relates to Component Extraction field, particularly a kind of banana skin polysaccharide extracts and purification process.
Background technology
Banana, as one of topmost cash crop of China, plants with a long history and annual production is large.People pay attention to pulp mostly, have ignored the value that Pericarpium Musae brings people.Pericarpium Musae accounts for greatly about 30% of its fruit, and at present, most Pericarpium Musae, directly as offal treatment, not only wastes resource but also contaminate environment.If the various functional ingredient in Pericarpium Musae can be developed, by being the new way solving Pericarpium Musae process, the economic worth of Pericarpium Musae can be improved again.Recent study shows, containing compounds such as flavonoid, phenols, organic acid and vegetable polysaccharidess in Pericarpium Musae.
Research shows, banana polysaccharide not only has therapeutic action but also have health value, as: there are the effects such as antiviral, anticancer, hypotensive, beauty treatment, emulsification clinically.Effectively can promote body immunity simultaneously, be again the integral part of cell and crganelle structure, be that human body cell or organoid maintain the indispensable main component of normal function, and have obvious restraining effect to cellular constituents of human milk.
Summary of the invention
Primary and foremost purpose of the present invention is to overcome the shortcoming that exists in prior art with not enough, provides a kind of banana skin polysaccharide to extract and purification process.
Another object of the present invention is to provide the banana skin polysaccharide obtained by said extracted and purification process thereof.
Object of the present invention is achieved through the following technical solutions: a kind of banana skin polysaccharide extracts and purification process (ultrasonic-microwave works in coordination with extraction method), comprise the steps: to get Pericarpium Musae sample, shake up after adding pure water, after 80 DEG C of immersion 30-90min, supersonic switch is opened, microwave irradiation power 60-100W, and ultrasonic-microwave is collaborative extracts 30-60min, centrifugal, get supernatant liquor; Filter residue is repeated extract, merge supernatant liquor, concentrating under reduced pressure; Get concentrated solution, add 5wt% trichoroacetic acid(TCA) and extract, centrifugal after vibration, get supernatant liquor, re-extract operates, until without obvious sediment, is merged by supernatant liquor, adds 0.3 times of volume 10wt%H
2o
2, leave standstill after vibration, filter, get filtrate, repetitive operation obtains Pericarpium Musae filtrate to filtrate is faint yellow to colourless, add the 95wt% ethanol of 3 times of volumes, in the centrifugal 10min of 9000r/min after 4 DEG C of precipitations, gets precipitation, obtains banana skin polysaccharide; 1 gram of Pericarpium Musae sample adds 10-20 milliliter pure water.
Described Pericarpium Musae sample is preferably adopted and is processed with the following method: cleaned up by Pericarpium Musae, dries to permanent quality, naturally dry in the shade after taking out in 60 DEG C, pulverize, cross 80 mesh sieves, use sherwood oil, 80wt% Ethanol Treatment successively, be dried to constant weight, obtain Pericarpium Musae sample.
Described centrifugal be in the centrifugal 15min of 4000r/min.
The described number of times repeating to extract is preferably 1-2 time.
After described vibration, centrifugal preferably adopting operates with the following method: after vibration 20min, the centrifugal 30min of 3000r/min.
The number of times of described re-extract operation is preferably 2-3 time.
The number of times of described repetitive operation is preferably 2-3 time.
Preferably, a kind of banana skin polysaccharide extracts and purification process (ultrasonic-microwave works in coordination with extraction method), comprise the steps: to get Pericarpium Musae sample, shake up after adding pure water, after 80 DEG C of immersion 60min, supersonic switch is opened, in 100W microwave radiation extraction 30min, centrifugal, get supernatant liquor; Filter residue is repeated extract, merge supernatant liquor, concentrating under reduced pressure; Get concentrated solution, add 5wt% trichoroacetic acid(TCA) and extract, centrifugal after vibration, get supernatant liquor, re-extract operates, until without obvious sediment, is merged by supernatant liquor, adds 0.3 times of volume 10wt%H
2o
2, leave standstill after vibration, filter, get filtrate, repetitive operation obtains Pericarpium Musae filtrate to filtrate is faint yellow to colourless, add the 95wt% ethanol of 3 times of volumes, in the centrifugal 10min of 9000r/min after 4 DEG C of precipitations, gets precipitation, obtains banana skin polysaccharide; 1 gram of Pericarpium Musae sample adds 20 milliliters of pure water.
Described Pericarpium Musae is preferably past the Pericarpium Musae of degreasing, pulverizing.
A kind of banana skin polysaccharide, is obtained by said extracted and purification process thereof.
The present invention has following advantage and effect relative to prior art: the present invention adopts ultrasonic-microwave to work in coordination with the polysaccharide extracted in Pericarpium Musae, its extraction yield is apparently higher than conventional backflow extraction method and be used alone supersonic method or be used alone microwave method, and ultrasonic-microwave works in coordination with extraction method, and to have selectivity good, efficiency is high, extraction time is short, reagent dosage is few, heat transfer time is fast, energy-conservation, easy and simple to handle, and security is good, and process such as easily to control at the advantage.Except cavitation effect, heat effect and mechanical effect that ultrasonic wave produces, microwave also has the characteristic of fluctuation, high frequency, enhance transmittance process, heat is converted to by microwave and is there is not the problem of transmission, thus realize the homogeneous heating of high strength, and the transmission of quality is strengthened due to the high speed swinging of molecule and the rising of temperature.The polar molecule that in plant, dielectric coefficient is larger simultaneously absorbs microwave and produces heat, there is vaporization or expand, interior osmotic pressure increases and destroys the cellularstructure of plant, diffusion duct is made to become large, shorten the broken time, strengthen the release of intracellular matter, diffusion and dissolving, thus significantly improve extraction efficiency.
Accompanying drawing explanation
Fig. 1 is the canonical plotting of glucose sugar.
Embodiment
Below in conjunction with embodiment and accompanying drawing, the present invention is described in further detail, but embodiments of the present invention are not limited thereto.
Experiment material and reagent:
Market is bought the banana of fresh mature, strip the clean Pericarpium Musae without rotting, Pericarpium Musae is cleaned, dry in 60 DEG C of thermostatic drying chambers to constant weight, taking-up is dried naturally, pulverizes, and crosses 80 mesh sieves, successively with sherwood oil process 2 times, removing lipid, then use 80wt% Ethanol Treatment 2 times, the materials such as removing monose, be dried to constant-quality, for subsequent use.
Glucose mark product (chromatographically pure, Pu Zhen bio tech ltd, Shanghai), methyl alcohol (AR), dehydrated alcohol (AR), propyl carbinol (AR), chloroform (AR), the vitriol oil (AR), phenol (AR), H
2o
2(AR), trichoroacetic acid(TCA) (AR).Above reagent is domestic product.
Laboratory apparatus:
XH-300A microwave catalysis synthesis/abstraction instrument (Beijing XiangHu Science and Technology Development Co., Ltd.), UV-2450 type ultraviolet-visible pectrophotometer (Japanese Shimadzu), 12E-5299 type thermostat water bath (Ya Xingtai mechanical & electronic equipment corporation, Ltd), SH-III type vacuum pump using circulatory water (Ying Yu high-tech instrument plant of Gongyi City), 80-3 table-type low-speed whizzer, FW-177 type crusher for Chinese herbal medicine (Tianjin Stettlen), RE-52A Rotary Evaporators (Shanghai Yarong Biochemical Instrument Plant), L-S electronic balance (0.1mg, plum Teller-Tuo benefit instrument company).
Embodiment 1
(1) banana skin polysaccharide extracts and a purification process, comprises the steps: to get Pericarpium Musae sample, shakes up after adding pure water, after 80 DEG C of immersion 60min, ultrasonic wave is opened, in 100W microwave extraction 30min, in the centrifugal 15min of 4000r/min, get supernatant liquor; Filter residue is repeated extraction 1 time, merge supernatant liquor, concentrating under reduced pressure; Get concentrated solution, add 5wt% trichoroacetic acid(TCA) and extract, after vibration 20min, the centrifugal 30min of 3000r/min, gets supernatant liquor, and re-extract operates 2 times, until without obvious sediment, is merged by supernatant liquor, adds 0.3 times of volume 10wt%H
2o
2, leave standstill after vibration, filter, get filtrate, repetitive operation 2 times, filtrate is faint yellow to colourless, obtains Pericarpium Musae filtrate, add the 95wt% ethanol of 3 times of volumes, in the centrifugal 10min of 9000r/min after 4 DEG C of precipitations, get precipitation (obtaining banana skin polysaccharide), by 80 DEG C of dissolved in purified water, solution is all transferred to constant volume after in 100mL volumetric flask, as test liquid sample; 1 gram of Pericarpium Musae sample adds 20 milliliters of pure water.The yield of banana skin polysaccharide is 96.7%.
(2) assay of banana skin polysaccharide:
The content of banana skin polysaccharide is detected with phend-sulphuric acid.Accurately pipette test liquid sample 1.00mL in 25mL tool plug scale test tube, mend to 2.0mL with pure water, order adds 5% phenol solution 1.0mL and vitriol oil 5.0mL, jolting is even, leave standstill 20min, be the interscan of 300-600nm scope with UV-2450 type ultraviolet-visible pectrophotometer at wavelength, find there is maximum absorption band at 490nm place, prove that polysaccharide exists.
(1) foundation of typical curve
Get glucose mark product to dry to constant-quality in baking oven (105 DEG C), accurately take 100mg, add dissolved in purified water and be settled to 10mL, be configured to the standardized solution that mass concentration is 10mg/mL.Accurately pipette respectively glucose standard 0.00,0.20,0.40,0.60,0.80,1.00mL is in 25mL tool plug scale test tube, respectively mend to 2.0mL with pure water, order adds 5% phenol solution 1.0mL and vitriol oil 5.0mL, jolting is even, leave standstill 20min, be mixed with successively mass concentration be respectively 0.00,0.08,0.16,0.24,0.32,0.40mgmL
-1serial solution, take reagent blank as reference, under 490nm wavelength, measures absorbancy, take absorbancy as X-coordinate (A), corresponding glucose content is ordinate zou (C), and drawing standard curve as shown in Figure 1, typical curve equation is: A=0.4061C-0.002, R
2=0.9987, show that dextrose standard sample is at 0.01 ~ 0.40ngmL
-1between, with absorbancy, there is linear relationship well.
(2) measurement of the polysaccharide content
Accurately pipette test liquid sample 0.1mL in 25mL volumetric flask, prepare sample solution according to the method for production standard curve solution and be settled to 25mL, with UV-2450 type ultra-violet and visible spectrophotometer, measure the absorbancy of wavelength 490nm place sample; As calculated, the content of banana skin polysaccharide is 7.61% (n=3).
Extracted amount (the gmL of polysaccharide
-1)=polysaccharide mass concentration (mgmL
-1) × 10
-3× extension rate
Polysaccharide content (%)=Polyose extraction amount × 100mL/ Pericarpium Musae sample quality
Embodiment 2
(1) banana skin polysaccharide extracts and a purification process, comprises the steps: to get Pericarpium Musae sample, shakes up after adding pure water, after 80 DEG C of immersion 30min, ultrasonic wave is opened, in 60W microwave extraction 40min, in the centrifugal 15min of 4000r/min, get supernatant liquor; Filter residue is repeated extraction 2 times, merge supernatant liquor, concentrating under reduced pressure; Get concentrated solution, add 5wt% trichoroacetic acid(TCA) and extract, after vibration 20min, the centrifugal 30min of 3000r/min, gets supernatant liquor, and re-extract operates 2 times, until without obvious sediment, is merged by supernatant liquor, adds 0.3 times of volume 10wt%H
2o
2, leave standstill after vibration, filter, get filtrate, repetitive operation 3 times, filtrate is faint yellow to colourless, obtains Pericarpium Musae filtrate, add the 95wt% ethanol of 3 times of volumes, in the centrifugal 10min of 9000r/min after 4 DEG C of precipitations, get precipitation (obtaining banana skin polysaccharide), by 80 DEG C of dissolved in purified water, solution is all transferred to constant volume after in 100mL volumetric flask, as test liquid sample; 1 gram of Pericarpium Musae sample adds 10 milliliters of pure water.The yield of banana skin polysaccharide is 93.6%.
(2) assay of banana skin polysaccharide:
The content of banana skin polysaccharide is detected with phend-sulphuric acid.Accurately pipette test liquid sample 1.00mL in 25mL tool plug scale test tube, mend to 2.0mL with pure water, order adds 5% phenol solution 1.0mL and vitriol oil 5.0mL, jolting is even, leave standstill 20min, be the interscan of 300-600nm scope with UV-2450 type ultraviolet-visible pectrophotometer at wavelength, find there is maximum absorption band at 490nm place, prove that polysaccharide exists.
(1) foundation of typical curve
Get glucose mark product to dry to constant-quality in baking oven (105 DEG C), accurately take 100mg, add dissolved in purified water and be settled to 10mL, be configured to the standardized solution that mass concentration is 10mg/mL.Accurately pipette respectively glucose standard 0.00,0.20,0.40,0.60,0.80,1.00mL is in 25mL tool plug scale test tube, respectively mend to 2.0mL with pure water, order adds 5% phenol solution 1.0mL and vitriol oil 5.0mL, jolting is even, leave standstill 20min, be mixed with successively mass concentration be respectively 0.00,0.08,0.16,0.24,0.32,0.40mgmL
-1serial solution, take reagent blank as reference, under 490nm wavelength, measures absorbancy, take absorbancy as X-coordinate (A), corresponding glucose content is ordinate zou (C), and drawing standard curve as shown in Figure 1, typical curve equation is: A=0.4061C-0.002, R
2=0.9987, show that dextrose standard sample is at 0.01 ~ 0.40mgmL
-1between, with absorbancy, there is linear relationship well.
(2) measurement of the polysaccharide content
Accurately pipette test liquid sample 0.1mL in 25mL volumetric flask, prepare sample solution according to the method for production standard curve solution and be settled to 25mL, with UV-2450 type ultra-violet and visible spectrophotometer, measure the absorbancy of wavelength 490nm place sample; As calculated, the content of banana skin polysaccharide is 7.18% (n=3).
Extracted amount (the gmL of polysaccharide
-1)=polysaccharide mass concentration (mgmL
-1) × 10
-3× extension rate
Polysaccharide content (%)=Polyose extraction amount × 100mL/ Pericarpium Musae sample quality
Embodiment 3
(1) banana skin polysaccharide extracts and a purification process, comprises the steps: to get Pericarpium Musae sample, shakes up after adding pure water, after 80 DEG C of immersion 90min, ultrasonic wave is opened, in 80W microwave extraction 60min, in the centrifugal 15min of 4000r/min, get supernatant liquor; Filter residue is repeated extraction 1 time, merge supernatant liquor, concentrating under reduced pressure; Get concentrated solution, add 5wt% trichoroacetic acid(TCA) and extract, after vibration 20min, the centrifugal 30min of 3000r/min, gets supernatant liquor, and re-extract operates 3 times, until without obvious sediment, is merged by supernatant liquor, adds 0.3 times of volume 10wt%H
2o
2, leave standstill after vibration, filter, get filtrate, repetitive operation 2 times, filtrate is faint yellow to colourless, obtains Pericarpium Musae filtrate, add the 95wt% ethanol of 3 times of volumes, in the centrifugal 10min of 9000r/min after 4 DEG C of precipitations, get precipitation (obtaining banana skin polysaccharide), by 80 DEG C of dissolved in purified water, solution is all transferred to constant volume after in 100mL volumetric flask, as test liquid sample; 1 gram of Pericarpium Musae sample adds 15 milliliters of pure water.The yield of banana skin polysaccharide is 89.4%.
(2) assay of banana skin polysaccharide:
The content of banana skin polysaccharide is detected with phend-sulphuric acid.Accurately pipette test liquid sample 1.00mL in 25mL tool plug scale test tube, mend to 2.0mL with pure water, order adds 5% phenol solution 1.0mL and vitriol oil 5.0mL, jolting is even, leave standstill 20min, be the interscan of 300-600nm scope with UV-2450 type ultraviolet-visible pectrophotometer at wavelength, find there is maximum absorption band at 490nm place, prove that polysaccharide exists.
(1) foundation of typical curve
Get glucose mark product to dry to constant-quality in baking oven (105 DEG C), accurately take 100mg, add dissolved in purified water and be settled to 10mL, be configured to the standardized solution that mass concentration is 10mg/mL.Accurately pipette respectively glucose standard 0.00,0.20,0.40,0.60,0.80,1.00mL is in 25mL tool plug scale test tube, respectively mend to 2.0mL with pure water, order adds 5% phenol solution 1.0mL and vitriol oil 5.0mL, jolting is even, leave standstill 20min, be mixed with successively mass concentration be respectively 0.00,0.08,0.16,0.24,0.32,0.40mgmL
-1serial solution, take reagent blank as reference, under 490nm wavelength, measures absorbancy, take absorbancy as X-coordinate (A), corresponding glucose content is ordinate zou (C), and drawing standard curve as shown in Figure 1, typical curve equation is: A=0.4061C-0.002, R
2=0.9987, show that dextrose standard sample is at 0.01 ~ 0.40mgmL
-1between, with absorbancy, there is linear relationship well.
(2) measurement of the polysaccharide content
Accurately pipette test liquid sample 0.1mL in 25mL volumetric flask, prepare sample solution according to the method for production standard curve solution and be settled to 25mL, with UV-2450 type ultra-violet and visible spectrophotometer, measure the absorbancy of wavelength 490nm place sample; As calculated, the content of banana skin polysaccharide is 6.92% (n=3).
Extracted amount (the gmL of polysaccharide
-1)=polysaccharide mass concentration (mgmL
-1) × 10
-3× extension rate
Polysaccharide content (%)=Polyose extraction amount × 100mL/ Pericarpium Musae sample quality
Comparative example
Adopt ultrasonic pass extraction method and ultrasonic extraction to carry out extracting the polysaccharide in Pericarpium Musae respectively, and measure the content of banana skin polysaccharide respectively.
(1) ultrasonic wave is closed extraction method and is extracted banana skin polysaccharide: a kind of banana skin polysaccharide extracts and purification process, comprise the steps: to get Pericarpium Musae sample, shake up after adding pure water, after 80 DEG C of immersion 60min, in 80W microwave radiation 30min, in the centrifugal 15min of 4000r/min, get supernatant liquor; Filter residue is repeated extraction 1 time, merge supernatant liquor, concentrating under reduced pressure; Get concentrated solution, add 5wt% trichoroacetic acid(TCA) and extract, after vibration 20min, the centrifugal 30min of 3000r/min, gets supernatant liquor, and re-extract operates 2 times, until without obvious sediment, is merged by supernatant liquor, adds 0.3 times of volume 10wt%H
2o
2, leave standstill after vibration, filter, get filtrate, repetitive operation 2 times, filtrate is faint yellow to colourless, obtains Pericarpium Musae filtrate, add the 95wt% ethanol of 3 times of volumes, in the centrifugal 10min of 9000r/min after 4 DEG C of precipitations, get precipitation (obtaining banana skin polysaccharide), by 80 DEG C of dissolved in purified water, solution is all transferred to constant volume after in 100mL volumetric flask, as test liquid sample; 1 gram of Pericarpium Musae sample adds 20 milliliters of pure water.The yield of banana skin polysaccharide is 67.1%.
(2) method of the present invention is adopted to extract banana skin polysaccharide: a kind of banana skin polysaccharide extracts and purification process, comprise the steps: to get Pericarpium Musae sample, shake up after adding pure water, after 80 DEG C of immersion 60min, ultrasonic wave is opened, in 100W microwave extraction 30min, in the centrifugal 15min of 4000r/min, get supernatant liquor; Filter residue is repeated extraction 1 time, merge supernatant liquor, concentrating under reduced pressure; Get concentrated solution, add 5wt% trichoroacetic acid(TCA) and extract, after vibration 20min, the centrifugal 30min of 3000r/min, gets supernatant liquor, and re-extract operates 2 times, until without obvious sediment, is merged by supernatant liquor, adds 0.3 times of volume 10wt%H
2o
2, leave standstill after vibration, filter, get filtrate, repetitive operation 2 times, filtrate is faint yellow to colourless, obtains Pericarpium Musae filtrate, add the 95wt% ethanol of 3 times of volumes, in the centrifugal 10min of 9000r/min after 4 DEG C of precipitations, get precipitation (obtaining banana skin polysaccharide), by 80 DEG C of dissolved in purified water, solution is all transferred to constant volume after in 100mL volumetric flask, as test liquid sample; 1 gram of Pericarpium Musae sample adds 20 milliliters of pure water.The content of banana skin polysaccharide is 7.61%.
Visible, the extraction yield of the banana skin polysaccharide adopting extracting method of the present invention to obtain is apparently higher than conventional backflow extraction method and be used alone supersonic method or be used alone microwave method, and ultrasonic-microwave works in coordination with extraction method, and to have selectivity good, efficiency is high, extraction time is short, reagent dosage is few, heat transfer time is fast, energy-conservation, easy and simple to handle, and security is good, and process such as easily to control at the advantage.And ultrasonic-microwave cooperating extracts except cavitation effect, heat effect and the mechanical effect that the polysaccharide in Pericarpium Musae produces except ultrasonic wave, microwave also has the characteristic of fluctuation, high frequency, enhance transmittance process, heat is converted to by microwave and is there is not the problem of transmission, thus realize the homogeneous heating of high strength, and the transmission of quality is strengthened due to the high speed swinging of molecule and the rising of temperature.The polar molecule that in plant, dielectric coefficient is larger simultaneously absorbs microwave and produces heat, vaporization occurs or expands, interior osmotic pressure increases and destroys the cellularstructure of plant, makes diffusion duct become large, strengthen the release of intracellular matter, diffusion and dissolving, thus significantly improve extraction efficiency.
Above-described embodiment is the present invention's preferably embodiment; but embodiments of the present invention are not restricted to the described embodiments; change, the modification done under other any does not deviate from spirit of the present invention and principle, substitute, combine, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (8)
1. a banana skin polysaccharide extracts and purification process, it is characterized in that, comprise the steps: to get Pericarpium Musae sample, shake up after adding pure water, after 80 DEG C of immersion 30-90min, supersonic switch is opened, microwave irradiation power 60-100W, ultrasonic-microwave is collaborative extracts 30-60min, centrifugal, gets supernatant liquor; Filter residue is repeated extract, merge supernatant liquor, concentrating under reduced pressure; Get concentrated solution, add 5wt% trichoroacetic acid(TCA) and extract, centrifugal after vibration, get supernatant liquor, re-extract operates, until without obvious sediment, is merged by supernatant liquor, adds 0.3 times of volume 10wt%H
2o
2, leave standstill after vibration, filter, get filtrate, repetitive operation obtains Pericarpium Musae filtrate to filtrate is faint yellow to colourless, add the 95wt% ethanol of 3 times of volumes, in the centrifugal 10min of 9000r/min after 4 DEG C of precipitations, gets precipitation, obtains banana skin polysaccharide; 1 gram of Pericarpium Musae sample adds 10-20 milliliter pure water.
2. banana skin polysaccharide according to claim 1 extracts and purification process, it is characterized in that, described Pericarpium Musae sample is adopted and is processed with the following method: cleaned up by Pericarpium Musae, dry to permanent quality in 60 DEG C, naturally dry in the shade after taking out, pulverize, cross 80 mesh sieves, use sherwood oil, 80wt% Ethanol Treatment successively, be dried to constant weight, obtain Pericarpium Musae sample.
3. banana skin polysaccharide according to claim 1 extracts and purification process, it is characterized in that, described centrifugal be in the centrifugal 15min of 4000r/min.
4. banana skin polysaccharide according to claim 1 extracts and purification process, it is characterized in that, the described number of times repeating to extract is 1-2 time.
5. banana skin polysaccharide according to claim 1 extracts and purification process, and it is characterized in that, after described vibration, centrifugal adopting operates with the following method: after vibration 20min, the centrifugal 30min of 3000r/min.
6. banana skin polysaccharide according to claim 1 extracts and purification process, it is characterized in that, the number of times of described re-extract operation is 2-3 time; The number of times of described repetitive operation is 2-3 time.
7. banana skin polysaccharide according to claim 1 extracts and purification process, it is characterized in that, a kind of extracting and purifying method of banana skin polysaccharide, comprise the steps: to get Pericarpium Musae sample, shake up after adding pure water, after 80 DEG C of immersion 60min, ultrasonic wave is opened, in 100W microwave extraction 30min, centrifugal, get supernatant liquor; Filter residue is repeated extract, merge supernatant liquor, concentrating under reduced pressure; Get concentrated solution, add 5wt% trichoroacetic acid(TCA) and extract, centrifugal after vibration, get supernatant liquor, re-extract operates, until without obvious sediment, is merged by supernatant liquor, adds 0.3 times of volume 10wt%H
2o
2, leave standstill after vibration, filter, get filtrate, repetitive operation obtains Pericarpium Musae filtrate to filtrate is faint yellow to colourless, add the 95wt% ethanol of 3 times of volumes, in the centrifugal 10min of 9000r/min after 4 DEG C of precipitations, gets precipitation, obtains banana skin polysaccharide; 1 gram of Pericarpium Musae sample adds 20 milliliters of pure water.
8. a banana skin polysaccharide, is obtained by the extraction described in any one of claim 1-7 and purification process thereof.
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| CN108578531A (en) * | 2018-03-26 | 2018-09-28 | 广西玉阳绿州生物科技有限公司 | Intestinal cancer healing liquid and its production technology |
| CN114230682A (en) * | 2022-02-08 | 2022-03-25 | 中国热带农业科学院分析测试中心 | Functional banana polysaccharide BPF2 and preparation and application thereof |
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