CN108570031A - A kind of extracting method of lycium ruthenicum anthocyanidin - Google Patents
A kind of extracting method of lycium ruthenicum anthocyanidin Download PDFInfo
- Publication number
- CN108570031A CN108570031A CN201710133587.7A CN201710133587A CN108570031A CN 108570031 A CN108570031 A CN 108570031A CN 201710133587 A CN201710133587 A CN 201710133587A CN 108570031 A CN108570031 A CN 108570031A
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- CN
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- Prior art keywords
- lycium ruthenicum
- anthocyanidin
- methanol
- extracting method
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/58—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4
- C07D311/60—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2
- C07D311/62—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2 with oxygen atoms directly attached in position 3, e.g. anthocyanidins
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B61/00—Dyes of natural origin prepared from natural sources, e.g. vegetable sources
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B67/00—Influencing the physical, e.g. the dyeing or printing properties of dyestuffs without chemical reactions, e.g. by treating with solvents grinding or grinding assistants, coating of pigments or dyes; Process features in the making of dyestuff preparations; Dyestuff preparations of a special physical nature, e.g. tablets, films
- C09B67/0096—Purification; Precipitation; Filtration
Abstract
The invention discloses a kind of extracting method of lycium ruthenicum anthocyanidin, belong to technical field of agricultural product process, the method includes the extraction of ultrasonic wave auxiliary reflux, resin column absorption, elution, extraction.The method of the present invention requires production equipment relatively low, and small to the destruction of anthocyanidin, recovery rate is high, agents useful for same pollution is small, can realize industrialized production.
Description
Technical field
The invention belongs to technical field of agricultural product process, and in particular to a kind of extracting method of lycium ruthenicum anthocyanidin.
Background technology
Lycium ruthenicum, wild Solanaceae Lycium, berry is spherical, thin skin, atropurpureus after skin is ripe, containing abundant inside fruit
Mauve pigment is highly soluble in water, belongs to natural water-soluble anthocyanin flavonoids.Tibetan medicine is for treating cardiopyretic disease, heart disease, reduction
Cholesterol, excited cerebral nerve, enhancing immune function, anti-curing cancers, anti-aging, beautifying face and moistering lotion, irregular menstruation, menopause etc. and medicine
Effect is notable.Civil to make strengthening by means of tonics kidney tonifying, decompression is medicinal.Recorded in《Uygur medicine will》,《Four doctor's allusion quotations》,《Jingzhubencao》
In equal Tibetan medicines classical works.Lycium ruthenicum is natural basic food, has the function of improving the acid-base property of human body.
Anthocyanidin, also known as anthocyanidin are the water-soluble natural pigments that nature one kind is widely present in plant, and it is yellow to belong to class
Ketone compound.Anthocyanidin is present in the vacuole of plant cell, can be transformed by chlorophyll.It is different in plant cell vacuole
Under pH value condition, make petal that multicoloured color be presented.Anthocyanidin is the nutritional supplement of pure natural anti-aging, research card
Bright is that mankind nowadays find that most effective antioxidant, its antioxygenic property are higher by 50 times than vitamin E, compare vitamin C
It is higher by 20 times.It is 100% to the biological effectiveness of human body, can be detected in blood within 20 minutes after taking.In addition
Anthocyanidin also has pre- anti-cancer, promotes eyesight, protection skin, improvement sleep and other effects.
103483304 A of Chinese patent CN disclose a kind of " process that anthocyanidin is extracted from lycium ruthenicum ", coagulate
Glue step is warming up to 78 DEG C -82 DEG C, however anthocyanidin is heat-sensitive substance, and temperature is more than 60 DEG C, and anthocyanidin can be destroyed.
105384716 A of Chinese patent CN disclose a kind of " method of enriching and purifying lycium ruthenicum anthocyanidin ", use inorganic acid water-soluble
Liquid extracts anthocyanidin, and recovery rate is low.
Invention content
The purpose of the present invention is to provide a kind of extracting methods of lycium ruthenicum anthocyanidin.This method produces simple, recovery rate
It is high, small to the destruction of anthocyanidin, solve the problems, such as in the prior art it is high to equipment requirement, recovery rate is low, anthocyanidin destructible.
A kind of extracting method of lycium ruthenicum anthocyanidin of the present invention, includes the following steps：
1) by lycium ruthenicum dry fruit coarse powder, 120 mesh sieve is crossed；
2) lycium ruthenicum after crushing is added into 60%-80% methanol, is extracted 3 times using ultrasonic wave auxiliary reflux, each 30-40
Minute, 50 DEG C of ultrasonic temperature, power 400kw；
3) extracting solution is adsorbed through macroporous absorbent resin, resin column blade diameter length ratio 1:8-1:12；
4) water-solubility impurity is removed, then washed with 70% methanol first with distillation 4-6 column volume of water elution under normal temperature and pressure
It is de-, collect eluent, thin layer chromatography detection；
5) eluent is concentrated under reduced pressure, ethyl acetate is added and extracts 3 times；
6) the lower mutually concentration of extraction, drying are to get lycium ruthenicum anthocyanidin finished product.
Compared with prior art, beneficial effects of the present invention：It is relatively low to production equipment requirement, small to the destruction of anthocyanidin,
Recovery rate is high, and agents useful for same pollution is small, can realize industrialized production.
Specific implementation mode
The present invention is elaborated further with reference to specific embodiment, but does not constitute and the scope of the present invention is limited
System.
Embodiment 1
A kind of extracting method of lycium ruthenicum anthocyanidin, includes the following steps：
1) 1kg lycium ruthenicum dry fruits are weighed, 120 mesh sieve is crossed after coarse powder；
2) lycium ruthenicum after crushing is added into 60% methanol, is extracted 3 times, 30 minutes every time, is surpassed using ultrasonic wave auxiliary reflux
Sound temperature 50 C, power 400kw；
3) extracting solution is adsorbed through macroporous absorbent resin, and resin column blade diameter length ratio is 1:12；
4) water-solubility impurity is removed, then eluted with 70% methanol first with distillation 4 column volumes of water elution under normal temperature and pressure,
Collect eluent, thin layer chromatography detection；
5) eluent is concentrated under reduced pressure, ethyl acetate is added and extracts 3 times；
6) the lower mutually concentration of extraction, drying are to get lycium ruthenicum anthocyanidin finished product 28.35g, content 82.93%.
Embodiment 2
A kind of extracting method of lycium ruthenicum anthocyanidin, includes the following steps：
1) 3kg lycium ruthenicum dry fruits are weighed, 120 mesh sieve is crossed after coarse powder；
2) lycium ruthenicum after crushing is added into 70% methanol, is extracted 3 times, 35 minutes every time, is surpassed using ultrasonic wave auxiliary reflux
Sound temperature 50 C, power 400kw；
3) extracting solution is adsorbed through macroporous absorbent resin, and resin column blade diameter length ratio is 1:10；
4) water-solubility impurity is removed, then eluted with 70% methanol first with distillation 5 column volumes of water elution under normal temperature and pressure,
Collect eluent, thin layer chromatography detection；
5) eluent is concentrated under reduced pressure, ethyl acetate is added and extracts 3 times；
6) the lower mutually concentration of extraction, drying are to get lycium ruthenicum anthocyanidin finished product 83.27g, content 85.42%.
Embodiment 3
A kind of extracting method of lycium ruthenicum anthocyanidin, includes the following steps：
1) 5kg lycium ruthenicum dry fruits are weighed, 120 mesh sieve is crossed after coarse powder；
2) lycium ruthenicum after crushing is added into 80% methanol, is extracted 3 times, 40 minutes every time, is surpassed using ultrasonic wave auxiliary reflux
Sound temperature 50 C, power 400kw；
3) extracting solution is adsorbed through macroporous absorbent resin, and resin column blade diameter length ratio is 1:8；
4) water-solubility impurity is removed, then eluted with 70% methanol first with distillation 6 column volumes of water elution under normal temperature and pressure,
Collect eluent, thin layer chromatography detection；
5) eluent is concentrated under reduced pressure, ethyl acetate is added and extracts 3 times；
6) the lower mutually concentration of extraction, drying are to get lycium ruthenicum anthocyanidin finished product 141.86g, content 83.05%.
Claims (1)
1. a kind of extracting method of lycium ruthenicum anthocyanidin, it is characterised in that include the following steps：
1) by lycium ruthenicum dry fruit coarse powder, 120 mesh sieve is crossed；
2) lycium ruthenicum after crushing is added into 60%-80% methanol, is extracted 3 times using ultrasonic wave auxiliary reflux, each 30-40 points
Clock, 50 DEG C of ultrasonic temperature, power 400kw；
3) extracting solution is adsorbed through macroporous absorbent resin, resin column blade diameter length ratio 1:8-1:12；
4) water-solubility impurity is removed, then eluted with 70% methanol first with distillation 4-6 column volume of water elution under normal temperature and pressure, received
Collect eluent, thin layer chromatography detection；
5) eluent is concentrated under reduced pressure, ethyl acetate is added and extracts 3 times；
6) the lower mutually concentration of extraction, drying are to get lycium ruthenicum anthocyanidin finished product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710133587.7A CN108570031A (en) | 2017-03-08 | 2017-03-08 | A kind of extracting method of lycium ruthenicum anthocyanidin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710133587.7A CN108570031A (en) | 2017-03-08 | 2017-03-08 | A kind of extracting method of lycium ruthenicum anthocyanidin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108570031A true CN108570031A (en) | 2018-09-25 |
Family
ID=63577776
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710133587.7A Pending CN108570031A (en) | 2017-03-08 | 2017-03-08 | A kind of extracting method of lycium ruthenicum anthocyanidin |
Country Status (1)
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| CN (1) | CN108570031A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109527177A (en) * | 2018-12-19 | 2019-03-29 | 九道通(北京)企业管理有限公司 | A kind of spot-eliminating beauty treatment tree peony lycium ruthenicum pressed candy |
| CN109527319A (en) * | 2018-12-19 | 2019-03-29 | 九道通(北京)企业管理有限公司 | Lycium ruthenicum Instant Drinks solid beverage with oxidation and removing free radicals |
| CN111777585A (en) * | 2020-07-24 | 2020-10-16 | 哈工大机器人南昌智能制造研究院 | Preparation method of high-purity lycium ruthenicum anthocyanin powder |
| CN111875573A (en) * | 2020-07-24 | 2020-11-03 | 哈工大机器人南昌智能制造研究院 | Method for continuously extracting anthocyanin from lycium ruthenicum murr |
-
2017
- 2017-03-08 CN CN201710133587.7A patent/CN108570031A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109527177A (en) * | 2018-12-19 | 2019-03-29 | 九道通(北京)企业管理有限公司 | A kind of spot-eliminating beauty treatment tree peony lycium ruthenicum pressed candy |
| CN109527319A (en) * | 2018-12-19 | 2019-03-29 | 九道通(北京)企业管理有限公司 | Lycium ruthenicum Instant Drinks solid beverage with oxidation and removing free radicals |
| CN111777585A (en) * | 2020-07-24 | 2020-10-16 | 哈工大机器人南昌智能制造研究院 | Preparation method of high-purity lycium ruthenicum anthocyanin powder |
| CN111875573A (en) * | 2020-07-24 | 2020-11-03 | 哈工大机器人南昌智能制造研究院 | Method for continuously extracting anthocyanin from lycium ruthenicum murr |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20180925 |