CN104940049B - Extracting method, extract and the application of yacon plant extracts - Google Patents
Extracting method, extract and the application of yacon plant extracts Download PDFInfo
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- CN104940049B CN104940049B CN201410124593.2A CN201410124593A CN104940049B CN 104940049 B CN104940049 B CN 104940049B CN 201410124593 A CN201410124593 A CN 201410124593A CN 104940049 B CN104940049 B CN 104940049B
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- yacon
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Abstract
The invention discloses a kind of extracting method of yacon plant extracts, including:In leaf or root tuber to pretreated yacon, ethanol or ethanol water are added as extractant, ultrasound, filtering, filtrate concentration;Concentrate is separated by macroporous resin column chromatography, column chromatography uses gradient elution, is first eluted with deionized water, and then with mass percent concentration, gradually increased ethanol water carries out gradient elution respectively successively, and post processing obtains extract.The invention also discloses the yacon plant extracts obtained by the above method and the application process of the extract.The present invention uses leaf or the root tuber of yacon as raw material, by simple extraction process, it can obtain the extract component with whitening function and anti-aging effect of different activities, can be according to being actually needed carry out categorised collection, and it is further prepared into required product, it is practical, there are wide market prospects.
Description
Technical field
The invention belongs to technical field of plant extraction, be specifically related to a kind of extracting method of yacon plant extracts,
Extract and as whitening active thing, anti-aging active thing and the application for including these whitening active things, anti-aging active thing.
Background technology
In the determinant of the colour of skin of people, the activity that human body produces the melanocyte of melanin occupies most important position
Put.Research shows that human melanocyte produces melanin by the activity of various enzymes (for example, tyrosinase), melanin
Forming process also by inherent cause, hormone secretion, with stress be relevant physiologic factor and environmental factor (for example, ultraviolet
Irradiation) influence.Melanin, which has, blocks solar ultraviolet, protects the skin organ below corium, removes and produced in skin histology
Raw free free radical, protect the effect of the protein and gene in skin.But because melanin is a kind of thing of stabilization
Matter, do not allow to be readily removable after stress disappear, until could remove from skin as the aging of horn cell comes off yet.Cause
This, if the hyperpigmentation for damaging beauty, example will occur because environmental factor generates a large amount of unnecessary melanin
Such as:Blackening, freckle and spot.With liking the crowd of outdoor activities to increase, it is excessively heavy by the radiation-induced melanin of UV to prevent
The demand also is increasing.In order to meet this demand, such as Vitamin C has been used in the skin preparations for extenal use such as cosmetics
Acid, kojic acid, ursin etc. have the material of tyrosinase inhibitory activity.But because whitening effect is undesirable and other asks
Topic (for example, above-mentioned whitening active thing is added to skin irritatin problem caused in cosmetics, formulation stability problems etc.),
So that the use of these compositions is restricted.Tyrosinase inhibitor from plant extracts is lived as potential whitening
Property thing, while there is security and the expection to skin low irritant again, at present report (the patent document of existing various exploitations
CN201310411195:The detection method of whitening efficacy of melothria heterophylla root extracts;CN201310382406.6:Suspension culture of Aquilaria sinensis pericarp extract
Application in skin-lightening cosmetic is prepared).
The reason for skin aging, can be divided into aging caused by light aging and internal cause, and light aging caused by UV exposures can cause
The activity increase of dermal elastin enzyme.Elastoser (Elastase) is distributed in Various Tissues and cell, elastin laminin
Enzymatic activity increase can cause the hydrolysis of elastic fibers of skin, so as to accelerate the decomposition of intradermal collagen and elastin laminin, make
Skin follows the string, and the skin ageing phenomenons such as wrinkle occurs.The anti-aging active thing for being currently known plant origin is flexible
Albumen enzyme inhibition, such as indigo plant (patent document CN200680023570.6:Skin preparations for extenal use), sweet pea (patent document
CN200680009020.9:Cosmetic preparation containing sweet pea extract) etc..
Yacon (Smallanthus sonchifolius (Poepp.&Endl.) H.Robinson) belongs to composite family sunflower
Platymiscium, South America andes region is originated in, introducing the ground such as Yunnan Province of China by Japan at the beginning of 21 century largely cultivates.Yacon original name
Ya Gong (yacon), Ya Gong, chrysanthemum potato, saussurea involucrata potato are called, are the traditional food of South America American Indian, have alleviation diabetes etc. more
The South America medical and edible dual purpose plant of kind effect.
Yacon root tuber contains substantial amounts of FOS, there is the appellation of " king of FOS ".Also contain in yacon root tuber
There are a variety of phenolic acid, terpene and flavone compound.Currently reported yacon leaves extract has anti-oxidant, removing free radical
The effect of.
The content of the invention
The invention provides a kind of extracting method of yacon plant extracts, the extracting method is simple to operate, suitable for work
Industry metaplasia is produced, and the extract for extracting to obtain by this method has good skin care and whitening effect.
Present invention also offers a kind of yacon plant extracts for extracting to obtain by the above method, the extract has good
Good skin care and whitening effect.
Present invention also offers a kind of application process of above-mentioned yacon plant extracts, it can be used as whitening and anti-aging skin
The active component of skin external preparation.
A kind of extracting method of yacon plant extracts, including:
(1) in the leaf to pretreated yacon or root tuber, ethanol or ethanol water are added as extractant,
Ultrasound, filtering, filtrate concentration;
(2) concentrate for obtaining step (1) carries out further separating-purifying by macroreticular resin, preferably using macropore
Resin column chromatography simultaneously uses gradient elution, is first eluted with deionized water, then successively respectively with mass percent concentration by
Gradually increased ethanol water carries out gradient elution, and post processing obtains extract.
In step (1), to improve extraction effect, described pretreatment mainly includes the steps such as cleaning, crushing.As excellent
Choosing, extractant use ethanol water, and the mass percent concentration of the ethanol water is 50-90%, more preferably
60-80%, it is still more preferably 70%.To ensure that active component as much as possible is extracted.
In step (1), the amount of the extractant once added be generally yacon leaf or 5-15 times of root tuber volume.It is super
The sonication time can determine that generally 0.5-2 hours, further is 1-2 hours according to being actually needed.Extraction time is general
It can be determined according to being actually needed, preferably, extraction is arrived three times twice, extraction every time is all ultrasonically treated.Extracting temperature can
Selection is carried out at room temperature, also can suitably be heated up.
In step (2), during macroporous resin column chromatography, preferably, described macroreticular resin is AB-8 macroporous absorptions
Resin.AB-8 macroporous absorbent resins are eluted with deionized water first, mainly remove some water-solubility impurities.Step (2)
In, preferably, it is 20-40% ethanol water, matter that the eluant, eluent of the gradient elution, which is followed successively by mass percent concentration,
Measure percent concentration be 60-80% ethanol water, mass percent concentration be 90-100% ethanol water, the same to time-division
Ethanol eluate is not collected, and three kinds of extracts are prepared after concentration respectively.As further preferred, described eluant, eluent successively
Ethanol water that ethanol water that respectively mass percent concentration is 30%, mass percent concentration are 70%, quality
Percent concentration is 95% ethanol water.Using the eluant, eluent of concentration stepped change, it is ensured that various in extract solution to have
Effect composition is separated, and the active component activity separated is different, during practical application, according to active different, to three kinds
The amount of composition is adjusted and selected, to meet the needs of different occasions.
Present invention also offers a kind of yacon plant extracts for extracting to obtain by the above method.The yacon plant carries
Thing is taken to be used in different occasions according to being actually needed.
Present invention also offers application process of several above-mentioned yacon plant extracts as skin preparations for extenal use.
The skin preparations for extenal use that a kind of yacon plant extracts acts in preparation with anti-oxidant, radicals scavenging is answered
With wherein the yacon plant extracts is prepared by above-mentioned various methods.
When the above-mentioned occasion of application, preferably, when the yacon plant extracts is the Ye Weiyuan using yacon
The eluted products for the ethanol water that mass percent concentration is 20-100% are collected during material, during gradient elution.When the saussurea involucrata
Fruit plant extracts is when using the root tuber of yacon as raw material, and it is 20-80% to collect mass percent concentration during gradient elution
Ethanol water eluted products.As still more preferably, the yacon plant extracts use the leaf of yacon for
Raw material, the eluted products for the ethanol water that mass percent concentration is 70-95% are collected during gradient elution.
A kind of yacon plant extracts is preparing the application of the skin preparations for extenal use with anti-aging effect, wherein the snow
Lotus fruit plant extracts is prepared by above-mentioned various methods.
When the above-mentioned occasion of application, preferably, collecting the ethanol that mass percent concentration is 60-80% during gradient elution
The eluted products of the aqueous solution.As further preferred, the root tuber of yacon is used as raw material.As still more preferably, gradient
The eluted products for the ethanol water that mass percent concentration is 70% are collected during elution;The root tuber of yacon is used simultaneously as original
Material.
A kind of yacon plant extracts is preparing the application of the skin preparations for extenal use with whitening function, wherein the saussurea involucrata
Fruit plant extracts is prepared by above-mentioned various methods.
When the above-mentioned occasion of application, preferably, collecting the second that mass percent concentration is 60-100% during gradient elution
The eluted products of alcohol solution.As further preferred, the leaf of yacon is used as raw material, collects quality percentage during gradient elution
Specific concentration is the eluted products of 70-95% ethanol water.Still more preferably it is, using the root tuber or Ye Weiyuan of yacon
Material, the eluted products for the ethanol water that mass percent concentration is 70% are collected during gradient elution.
As to the preferred of above-mentioned several schemes, described skin preparations for extenal use is cosmetics, particularly Essence cosmetics,
The percentage by weight that Smallanthus sonchifolius fruit extract accounts in Essence is 1-10%, more preferably 1-7%.
Composite family Helianthus plant, preferably yacon (Smallanthus are used in the present invention
sonchifolius(Poepp.&Endl.)H.Robinson)。
Compared with prior art, beneficial effects of the present invention are embodied in:
The present invention uses leaf or the root tuber of yacon as raw material, by simple extraction process, you can obtain different work
Property the extract component with whitening function and anti-aging effect, can be according to being actually needed carry out categorised collection, and enter one
Step is prepared into required product, practical, has wide market prospects.
Embodiment
Embodiment 1:The preparation of Smallanthus sonchifolius fruit extract
The yacon leaves or root tuber produced in the present embodiment using Yunnan, but not limited to this.In addition, carried in embodiment
The percentage composition arrived, in the case of no specified otherwise, equal representation quality percent concentration.
(1) 70% ethanol 1000mL is added in the leaf of 100g yacons, extraction 2 time ultrasonic 1 hour in room temperature, then
Filtering.The solvent under reduced pressure of filtrate is concentrated into 50ml, upper AB-8 resin columns, successively with deionized water, 30% ethanol, 70% ethanol
With 95% ethanol elution (each 500ml), it is evaporated under reduced pressure after collecting respectively and removes solvent, obtained drying solid, weigh, obtain saussurea involucrata
30% ethanol position 2.95g of fruit leaf, 70% ethanol position 9.89g, 95% ethanol position 2.45g.
(2) 70% ethanol 1000mL is added in the root tuber of 100g yacons, extraction 2 time ultrasonic 1 hour in room temperature, so
After filter.The solvent under reduced pressure of filtrate is concentrated into 50ml, upper AB-8 resin columns, successively with deionized water, 30% ethanol, 70% second
Alcohol and 95% ethanol elution (each 500ml), it is evaporated under reduced pressure after collecting respectively and removes solvent, obtained drying solid, weigh, avenged
30% ethanol position 3.0g of lotus fruit block root, 70% ethanol position 3.6g, 95% ethanol position 1.0g.
Embodiment 2:The detection of antioxidation activity
Smallanthus sonchifolius fruit extract is prepared according to embodiment 1, its free-radical scavenging activity is determined with DPPH methods.Determination step is:
Smallanthus sonchifolius fruit extract is configured to the aqueous solution (mass-volume concentration 0.05mg/mL) with deionized water respectively, pipette 2mL in
In 10mL tool plug test tubes, adding people 2mL DPPH (1,1- diphenyl -2- trinitrophenyl-hydrazines) ethanol solution, (molal volume concentration is 2*
10-4Mol/L), fully mix, be stored at room temperature, the absorbance T of spectrophotometric determination 517nm wavelength is used after 30min;Survey simultaneously
Determine 2mL DPPH solution and the mixed absorbance C of 2mL ethanol, 2mL deionized waters and the mixed absorbance C of 2L ethanol0, with
And 2mL extract solutions and the mixed absorbance T of 2mL ethanol solutions0.Parallel determination three times, is averaged, according to below equation meter
Calculate free radical scavenging activity.
Free radical scavenging activity=[1- (T-T0)/(C-C0)] × 100%.
The measurement result of table 1DPPH method free radical scavenging activities
As shown in Table 1, in the Smallanthus sonchifolius fruit extract used in the present invention, any solvent extractable matter be respectively provided with it is excellent from
It is useful as age resister by base scavenging action.During especially with yacon leaves as extraction material, eluant, eluent uses
During 70%-95% ethanol water, its free radical scavenging activity is best.Therefore, Smallanthus sonchifolius fruit extract can be coordinated in external application
In agent, used as the anti-aging active thing of skin ageing, the skin state of maintenance young healthy is prevented.
Embodiment 3:The detection of elastin laminin enzyme level
The Smallanthus sonchifolius fruit extract prepared respectively according to embodiment 1, is dissolved in deionized water, is configured to the aqueous solution (quality volume
Concentration is 0.5mg/ml), for determining elastase inhibitory activity.Determination step is:10 μ L samples are added in 96 orifice plates
Solution and 130 μ L contain 1.015mM reaction substrates N- succinyls-propionyl ammonia-propionyl ammonia-propionyl ammonia-paranitroanilinum (English name
Succ-Ala-Ala-Ala-pNA, No. CAS:0.1M Tris-Cl cushioning liquid (pH8.0) 52299-14-6), at 25 DEG C
Incubate 5 minutes, add 15 μ L elastin laminins enzyme solutions (0.5U/ml), continue to incubate 30min at 25 DEG C, then use ELIASA
Determine the absorbance A of 410nm wavelength410.Sample aqueous solution is replaced with deionized water, absorbance is equally determined as reference solution,
It the results are shown in Table 2.
Elastase activity inhibiting rate calculation formula is:Inhibiting rate (%)=(1- (A410-C0)/A0) × 100%, wherein
A0It is the absorbance of reference solution, A410It is the absorbance of sample solution, C0It is the absorbance of sample solution background.
The measurement result of the elastin laminin enzyme level of table 2
As shown in Table 2, in the Smallanthus sonchifolius fruit extract used in the present invention, the ethanol position of yacon root tuber 70% and saussurea involucrata
The ethanol position of fruit leaf 70%, it is respectively provided with and suppresses elastin laminin enzyme effect, yacon root tuber and yacon leaves extract can be matched somebody with somebody
Close in external preparation, as preventing skin ageing, maintain the age resister of skin shape of young healthy to use.
Embodiment 4:The detection that tyrosinase activity suppresses
The Smallanthus sonchifolius fruit extract prepared respectively according to embodiment 1, is dissolved in deionized water, is configured to the aqueous solution (quality volume
Concentration is 0.5mg/ml), for determining tyrosinase inhibitory activity, using ursin as object of reference.Determination step is:Phosphate delays
Rush μ L of solution (pH6.8,30mmol/L) 70, μ L of 1.66mmol/L tyrosine solutions 80, the μ L of sample solution 80 to be well mixed, 37
Incubated 5 minutes in DEG C Water Tank with Temp.-controlled, tyrosinase solution (1000U/ml) 10 μ L are added, after 37 DEG C of constant temperature 10min, with enzyme mark
Instrument determines the absorbance A of 475nm wavelength475.Sample aqueous solution is replaced with deionized water, extinction is equally determined as reference solution
Degree, the results are shown in Table 3.
Inhibitory activity against tyrosinase calculation formula is:Inhibiting rate (%)=(1- (A475-C0)/A0) × 100%, wherein A0
It is the absorbance of reference solution, A475It is the absorbance of sample solution, C0It is the absorbance of sample solution background.
The measurement result of the tyrosinase inhibitory activity of table 3
As shown in Table 3, each Smallanthus sonchifolius fruit extract used in the present invention all has tyrosinase activity inhibitory action.Its
In, the ethanol position of yacon root tuber 70% and the ethanol position of yacon leaves 70% suppression tyrosinase effect are stronger and all excellent
In the positive reference substance ursin of same concentrations, yacon root tuber and yacon leaves extract can be coordinated in external preparation,
Used as the pigmented whitening active thing of skin is prevented.
Embodiment 5:Skin preparations for extenal use containing Smallanthus sonchifolius fruit extract
70% ethanol 1000mL is added in the root tuber of 100g yacons, in room temperature ultrasound 1 hour, is extracted 2 times, then mistake
Filter.The solvent under reduced pressure of filtrate is concentrated into 50ml, upper AB-8 resin columns, successively with deionized water, 30% ethanol, 70% ethanol and
95% ethanol elution (each 500ml), removing solvent is evaporated under reduced pressure after collecting 70% alcohol elution, obtains yacon root tuber
70% ethanol position, the preparation for skin preparations for extenal use.Percentage by weight of the Smallanthus sonchifolius fruit extract in skin preparations for extenal use
It is 1-10%, preferably 1-7%.It is the embodiment of concrete application of the yacon root tuber in skin preparations for extenal use below.In table 4 below
"-" represents no added.
Embodiment 6:Essence containing yacon root tuber
Yacon root tuber extract is taken, for preparing the Essence with whitening function, the component that the Essence contains
As shown in table 4:
Table 4
Above is invention preferred embodiment, is not intended to limit the invention.
Claims (8)
1. a kind of extracting method of yacon plant extracts, including:
(1) in the leaf to pretreated yacon or root tuber, add ethanol or ethanol water is used as extractant, it is super
Sound, filtering, filtrate concentration;
(2) concentrate for obtaining step (1) carries out the further separating-purifying of column chromatography by macroreticular resin, and uses gradient
Elution, is first eluted, then with mass percent concentration, gradually increased ethanol water enters respectively successively with deionized water
Row gradient elution, post processing obtain extract;
Described macroreticular resin is AB-8 macroporous absorbent resins;
It is 20-40% ethanol water, mass percent that the eluant, eluent of the gradient elution, which is followed successively by mass percent concentration,
The ethanol water that ethanol water that concentration is 60-80%, mass percent concentration are 90-100%.
2. a kind of yacon plant extracts, it is characterised in that the yacon plant extracts is as the side described in claim 1
Method is extracted to obtain.
3. a kind of yacon plant extracts is preparing the application of skin preparations for extenal use, it is characterised in that the yacon plant carries
Thing is taken to be prepared as the method described in claim 1.
4. yacon plant extracts according to claim 3 is preparing the application of skin preparations for extenal use, it is characterised in that institute
The skin preparations for extenal use stated is cosmetics.
5. yacon plant extracts according to claim 4 is preparing the application of skin preparations for extenal use, it is characterised in that institute
The skin preparations for extenal use stated is Essence cosmetics, and the percentage by weight that Smallanthus sonchifolius fruit extract accounts in Essence is 1-10%.
6. yacon plant extracts according to claim 3 is preparing the application of skin preparations for extenal use, it is characterised in that when
The yacon plant extracts is when using the leaf of yacon as raw material, and collecting mass percent concentration during gradient elution is
The eluted products of 20-100% ethanol water;When the yacon plant extracts be use yacon root tuber for raw material
When, the eluted products of the ethanol water that mass percent concentration is 20-80% are collected during gradient elution.
7. yacon plant extracts according to claim 3 is preparing the application of skin preparations for extenal use, it is characterised in that adopts
It is raw material with the root tuber of yacon;The elution for the ethanol water that mass percent concentration is 60-80% is collected during gradient elution
Product.
8. yacon plant extracts according to claim 3 is preparing the application of skin preparations for extenal use, it is characterised in that adopts
It is raw material with the root tuber or leaf of yacon, the ethanol water that mass percent concentration is 70-95% is collected during gradient elution
Eluted products.
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EA201891183A1 (en) * | 2015-11-18 | 2018-12-28 | СИБЕРГ КОНСАЛТИНГ, ЭлЭлСи | COMPOSITIONS CONTAINING NATURAL EXTRACTS AND THEIR APPLICATION FOR SKIN AND HAIR |
CN107137655A (en) * | 2017-06-09 | 2017-09-08 | 刘丽华 | It is a kind of for pharmaceutical composition of dysentery and preparation method thereof |
CN107468576A (en) * | 2017-08-30 | 2017-12-15 | 上海应用技术大学 | A kind of composition containing burdock extract and its application in cosmetics |
US11020340B2 (en) | 2017-09-18 | 2021-06-01 | Seiberg Consulting, LLC | Compositions containing natural products and use thereof for skin and hair |
CN109806195A (en) * | 2017-11-22 | 2019-05-28 | 广州慕可生物科技有限公司 | A kind of composite plant extract liquid moisture-keeping composition and preparation method thereof |
CN107998033B (en) * | 2017-12-28 | 2020-06-30 | 珠海伊斯佳科技股份有限公司 | Composition with anti-aging function and application of composition in cosmetics |
CN108553498A (en) * | 2018-04-24 | 2018-09-21 | 烟台金蕊女性用品有限公司 | A kind of saussurea involucrata essence extraction process |
CN109275778A (en) * | 2018-08-30 | 2019-01-29 | 苏州佩普肽康生物科技有限公司 | A kind of women helps pregnant food formula of special diet and preparation method thereof |
CN115028751B (en) * | 2022-06-15 | 2023-05-16 | 中国科学院新疆理化技术研究所 | Preparation method and application of saussurea involucrata polysaccharide |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1826989A (en) * | 2006-04-04 | 2006-09-06 | 昆明瑞鹏生态农业科技有限公司 | Functional drink with yacon and preparation process thereof |
FR2907338A1 (en) * | 2006-10-20 | 2008-04-25 | Greentech | Use of ferulic acid, its salts and/or derivatives as lipolytic agent to prepare a cosmetic and/or pharmaceutical composition to prevent and/or treat the adipose overloads and/or cellulitis, by systemic and/or local administration |
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-
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Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1826989A (en) * | 2006-04-04 | 2006-09-06 | 昆明瑞鹏生态农业科技有限公司 | Functional drink with yacon and preparation process thereof |
FR2907338A1 (en) * | 2006-10-20 | 2008-04-25 | Greentech | Use of ferulic acid, its salts and/or derivatives as lipolytic agent to prepare a cosmetic and/or pharmaceutical composition to prevent and/or treat the adipose overloads and/or cellulitis, by systemic and/or local administration |
Non-Patent Citations (1)
Title |
---|
雪莲果叶中酚酸的分离鉴定及其生物活性研究;陈红惠;《中国优秀硕士学位论文全文数据库医药卫生科技辑》;20100715(第7期);第15、16、18、30、32、40、50、63-64页 * |
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