CN106137812A - Radix Gentianae extract and its production and use - Google Patents
Radix Gentianae extract and its production and use Download PDFInfo
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Abstract
The invention discloses Radix Gentianae extract and its production and use.The preparation method of this Radix Gentianae extract comprises the steps: that Radix Gentianae is mixed by (1) with solvent, extracts, and filters, removed under reduced pressure solvent, i.e. can get Radix Gentianae crude extract；(2) after Radix Gentianae crude extract being dissolved in water, it is loaded to resin packed column, successively with water and ethanol solution eluting, collects eluent respectively, removed under reduced pressure second alcohol and water,.The preparation method of the Radix Gentianae extract of the present invention is simple, and the Radix Gentianae extract prepared can be as tyrosinase inhibitor, elastase inhibitor, antioxidant activity agent and the application of anti-aging actives；The further preferably application in preparing skin care item or cosmetics.
Description
Technical field
The present invention relates to field of plant extraction, be specifically related to a kind of Radix Gentianae extract and preparation method thereof and use
On the way.
Background technology
Within 1956, Harman proposes free radical causes of senescence [Harman, D.Aging:A theory based
On free radical and radiation chemistry.J.Gerontol.1956,11:289-300.], it is thought
Too much free radical is the major reason causing biological aging.Theoretical according to this, the activity of internal excess
Oxygen-derived free radicals and unsaturated fatty acid effect produce the materials such as malonaldehyde, malonaldehyde by with the egg on cell membrane
The effect such as white matter produces brown pigment, is deposited in skin and becomes as various mottles.The free radical of excess can also make
Collagen fiber in skin, elastic fiber crosslink with degeneration, become fragile, follow the string, work as skin beauty water
When dividing not enough, easily make elastic fibers break, the skin ageing phenomenons such as dark stricture of vagina, microgroove, wrinkle occur.
Additionally, the ionizing radiation in environment and the environmental contaminants of such as air pollution and chemical substance etc., also
Free radical is constantly produced in making organism.Such as ultraviolet in environment can make the fibre in dermis of skin
Dimension blast cell and grain wire body are upset, and then discharge superoxide anion, and the superoxide anion of excess
Then can be converted into other destructive higher free radical.It is able to maintain that oxidation and antioxygen although having in human body
The Antioxidative Defense System of poised state between change, in order to slow down the generation of active oxygen and free radical, if but
Be the most excessive to tan by the sun in the sun, then in human body, a large amount of free radicals produced can cause the antioxygen of skin
Change defence capability to reduce, cause the injury of skin, such as photoaging, creasy surface, skin immunization power to lose
Adjust.Antioxidant is the material having and catching free radical ability, can remove the living radical in skin,
Alleviate the skin oxidative stress damage that free radical causes, thus it is old to improve the skin caused by oxidative stress etc.
Change.The removing free radical type antioxidant being currently known in the organism such as vitamin E, vitamin C,
The most also (the patent documentations such as the antioxidant such as Flos Nelumbinis of plant origin, prunus mume (sieb.) sieb.et zucc. berry extract are reported
CN201110158540.9 contains Flos Nelumbinis extract and Fructus Mume extract and has antioxygen as effective ingredient
Change the Dermatologic preparation composition of activity).
About the formation of dermal melanin, current study show that mainly due to the bioid in melanocyte
Reaction causes, and i.e. tyrosine is under the effect as the tryrosinase of catalyst, generates DOPA quinone, then
Melanin is formed by the catalytic action of enzyme or the Oxidation of non-enzymatic.Generate about suppressing above-mentioned melanin
Whitening active thing, generally with suppression tyrosinase activity as major target class.Derive from plant extract
Tyrosinase inhibitor, as potential whitening active thing, has again safety and stimulation low to skin simultaneously
Property expection, the at present report of existing various exploitations, such as patent documentation CN201310411195.4 is white
The detection method of liana extract white-skinned face function；CN201310382406.6 Lignum Aquilariae Resinatum peel extract is in system
Application in standby skin-lightening cosmetic.
It is aging that the reason of skin aging can be divided into that photoaging and endogenous cause of ill cause, and UV exposes the light caused
Aging meeting causes the activity increase of dermal elastin enzyme.Elastoser (Elastase) is distributed in multiple
In tissue and cell, elastase activity increase may result in the hydrolysis of elastic fibers of skin, thus accelerates
Intradermal collagen protein and the decomposition of elastin laminin, make skin follow the string, the skin aginges such as wrinkle occur
Phenomenon.The anti-aging active thing being currently known plant origin has Elastase Inhibitory, such as knotweed
Blue (patent documentation CN200680023570.6 skin preparations for extenal use), sweet pea (patent documentation
CN200680009020.9 contains the cosmetic preparation of sweet pea extract) etc..
Gentiana (Gentiana (Tourn.) L.) plant in China's Mainland, Russia, Japan, Korea etc.
All there is distribution, be grown on height above sea level 400 meters to 1, the area of 700 meters.In state-owned Gentiana kind more than 240,
Originating in alpine region, southwest, Beijing periphery only has a few, with Cuculus polioephalus, heralding spring is collectively referred to as the world three more
Big Alpine Wild Ornamental Plants.Radix Gentianae is also important medicinal plants, its root and rhizome be used as medicine have heat clearing away, eliminating pathogen in the liver,
Effect of arresting convulsion.Gentiana lutea contains the compounds such as gentiopicrin, erythricine, triterpenes.Have no that Radix Gentianae is made
Answering for tyrosinase inhibitor, elastase inhibitor, antioxidant activity agent and anti-aging actives
Report.
Summary of the invention
The technical problem to be solved there is provided a kind of Radix Gentianae extract and preparation method thereof and
Purposes.The preparation method of the Radix Gentianae extract of the present invention is simple, and the Radix Gentianae extract prepared can be as cheese
Propylhomoserin enzyme inhibitor, elastase inhibitor, antioxidant activity agent and the application of anti-aging actives；
The further preferably application in preparing skin care item or cosmetics.
The invention provides the preparation method of a kind of Radix Gentianae extract, it is any one that it uses in following method
Kind,
Method one, comprising the steps: to mix Radix Gentianae with solvent, extract, filter, removed under reduced pressure is molten
Agent,；
Method two, comprise the steps:
(1) Radix Gentianae is mixed with solvent, extract, filter, removed under reduced pressure solvent, i.e. can get Radix Gentianae
Crude extract；
(2), after Radix Gentianae crude extract being dissolved in water, it is loaded to resin packed column, successively with water and ethanol
Eluant solution, collects eluent respectively, removed under reduced pressure second alcohol and water,.
Wherein, described Radix Gentianae is generally Gentiana (Gentiana (Tourn.) L.) plant, preferably
Alpine gentian (Gentiana algida Pall.), Gentiana veitchiorum (Gentiana Veitchiorum Hemsl),
Changbai Mountain Radix Gentianae (Gentiana jamesii Hemsl.), Flos Caryophylli Radix Gentianae (Gentiana szechenyii Kanitz),
Dali Radix Gentianae (Gentiana taliensis Balf.f.&Forrest), spend more Radix Gentianae (Gentiana striolata T.
N.Ho), noble Radix Gentianae (Gentiana gentilis Franch), red Radix Gentianae (Gentiana rhodantha
Franch.ex Hemsl.), Hemerocallis citrina Baroni Radix Gentianae (Gentiana flavomaculata Hayata), leaf of Herba hylotelephii erythrosticti Radix Gentianae
(Gentiana crassula H.Smith), Radix Gentianae sarcorrhizae (Gentiana sarcorrhiza Ling&Ma ex T.N.
Ho), Linzhi's Radix Gentianae (Gentiana nyingchiensis T.N.Ho), fringed gentian (Gentiana panthaica
Prain&Burk.), beautiful Radix Gentianae (Gentiana formosa H.Smith), Radix Gentianae Macrophyllae (Gentiana
Macrophylla Pall.), Qinghai-Tibet Radix Gentianae (Gentiana futtereri Diels&Gilg), silk calyx Radix Gentianae
(Gentiana filisepala T.N.Ho), Tianshan Mountains Radix Gentianae Macrophyllae (Gentiana tianschanica Rupr.), Western Regions dragon
Gallbladder (Gentiana clarkei Kusnez.), Sikkim gentian (Gentiana sikkimensis C.B.Clarke), happiness
The refined Radix Gentianae of horse traction (Gentiana venusta (G.Don) Wall.ex Griseb.), little Radix Gentianae (Gentiana
Parvula H.Smith), Xinjiang Radix Gentianae (Gentiana karelinii Griseb.), Xinjiang Radix Gentianae Macrophyllae (Gentiana
Walujewii Regel&Schmalh.), beautiful Radix Gentianae (Gentiana bella Franch.ex Hemsl.), silver
Calyx Radix Gentianae (Gentiana albicalyx Burk.), Yushan Radix Gentianae (Gentiana scabrida Hayata), Yunnan
Radix Gentianae (Gentiana yunnanensis Franch.), China Radix Gentianae (Gentiana chinensis Kusnez.),
In Mount Everest Radix Gentianae (Gentiana stellata Turrill) and Gentiana (Gentiana syringea T.N.Ho)
One or more, be more preferably alpine gentian (Gentiana algida Pall.).
Wherein, in step (1), described extraction is preferably supersound extraction, stirring is extracted or adverse current
Circulation is extracted, and the time of described supersound extraction is preferably 0.5～1.5 hour, and described stirring is extracted
Time be preferably 1.5～2 hours, it is little that the time that described circulated in countercurrent is extracted is preferably 0.3～1
Time.
Wherein, in step (1), described preferred solvents ground is ethanol that percent by volume is 50～95%
Aqueous solution, is more preferably the ethanol water of 70% for percent by volume.
Wherein, the volume mass ratio preferably 15～30mL/g of described Radix Gentianae and solvent.
Wherein, described water is preferably deionized water.
Wherein, in step (2), described ethanol solution eluting is preferably successively by 10%～50% second
Alcohol-water solution, 51%～90% ethanol water and 91%～99% ethanol water carry out Concentraton gradient eluting,
More preferably for carry out dense with 30% ethanol water, 70% ethanol water and 95% ethanol water successively
Degree gradient elution；Percentage ratio is percent by volume.
Wherein, described resin can be macroporous resin commonly used in the art, preferably D101 tree
Fat or AB-8 resin.
Present invention also offers the Radix Gentianae extract obtained by above-mentioned preparation method, described Radix Gentianae extract
The extract that preferably 51%～90% ethanol water eluting obtains；It is more preferably that 70% ethanol is water-soluble
The extract that liquid affords；Percentage ratio is percent by volume.
Present invention also offers above-mentioned Radix Gentianae extract as tyrosinase inhibitor, elastin laminin enzyme level
Agent, antioxidant activity agent or the application of anti-aging actives；Further preferably preparing skin care item or cosmetic
Application in product.
On the basis of meeting common sense in the field, above-mentioned each optimum condition, can combination in any, i.e. get Ben Fa
Bright each preferred embodiments.
Agents useful for same of the present invention and raw material are the most commercially.
The most progressive effect of the present invention is: the preparation method of the Radix Gentianae extract of the present invention is simple, system
The Radix Gentianae extract obtained can be as non-diseases diagnosis and/or the tyrosinase inhibitor of therapeutic use, elasticity
Protease inhibitor, antioxidant activity agent and the application of anti-aging actives, can join Radix Gentianae extract
It is combined in external preparation, as preventing skin ageing, maintaining the age resister of the skin state of young healthy to make
With.
Detailed description of the invention
Further illustrate the present invention below by the mode of embodiment, but the most therefore limit the present invention to
Among described scope of embodiments.The experimental technique of unreceipted actual conditions in the following example, according to often
Rule method and condition, or select according to catalogue.
Embodiment 1
The preparation of alpine gentian extract
By 50g alpine gentian (Gentiana algida Pall.) crushed after being dried, with 1000mL 70%
Ethanol solution (v/v, in the present invention, all ethanol solution are volumetric concentration) mixing is soaked, and ultrasonic wave added carries
Taking 1 hour, filter, filtrate, after removed under reduced pressure solvent, obtains alpine gentian crude extract 7.0g.
Take alpine gentian crude extract, after deionized water dissolving, be loaded to AB-8 resin packed column, depend on
(percentage ratio is volume hundred for secondary deionized water, 30% ethanol, 70% ethanol and 95% ethanol gradient elution
Proportion by subtraction), collect eluent, removed under reduced pressure second alcohol and water respectively, weigh, respectively obtain water elution position
4.7g, 30% alcohol elution 0.6g, 70% alcohol elution 1.0g and 95% alcohol elution
The alpine gentian extract of 0.7g.
Embodiment 2
The mensuration of general flavone content in alpine gentian extract
Alpine gentian extract embodiment 1 prepared, according to Pharmacopoeia of People's Republic of China (2010
Version) method, with rutin as standard substance, measure general flavone content in alpine gentian extract.
Determination step is: 1) standard curve making: precision measure standard solution 1ml, 2ml, 3ml,
4ml, 5ml, 6ml, put in 25ml measuring bottle respectively, respectively adds 30% ethanol to 6.0ml, adds 5% nitrous
Acid sodium solution (mass percent concentration) 1ml.Mixing, places 6 minutes, then it is molten to add 10% aluminum nitrate
Liquid (mass percent concentration) 1ml, shakes up, and places 6 minutes.Hydro-oxidation sodium test solution 10ml, then
Add 30% ethanol to scale, shake up, place 15 minutes, with corresponding reagent as blank, use ultraviolet-visible
Spectrophotography, measures absorbance at the wavelength of 510nm, and with absorbance as vertical coordinate, concentration is horizontal
Coordinate, draws standard curve.
2) the alpine gentian extract at each eluting position that Example 1 prepares is completely dissolved in deionized water,
It is configured to 1mg/mL aqueous solution.Being placed in 25ml volumetric flask by a certain amount of sample, other is according to standard
Curve preparation process, from " adding 5% sodium nitrite solution 1ml ", operates same step 1), surveys successively
Determining absorbance, take sample solution 3ml simultaneously, in addition to being not added with sodium hydroxide test solution, remaining ibid operates,
As blank, calculate general flavone content (in terms of rutin) in alpine gentian extract, as shown in table 1 below.
Table 1 alpine gentian extractive total flavone content
From table 1, the crude extract obtained after alpine gentian is extracted contains more flavone compound,
Flavone is the main active of alpine gentian, and after adsorbent resin separates, alpine gentian 70% ethanol is washed
The flavone of de-position enrichment is most, improves a lot than flavones content in crude extract, its biological activity also phase
Should strengthen.
Embodiment 3
The detection of tyrosinase inhibitory activity
The alpine gentian extract at each eluting position embodiment 1 prepared is completely dissolved in deionized water, joins
Make 1.0mg/mL aqueous solution, i.e. sample solution, be used for measuring tyrosinase inhibitory activity, with Folium Vaccinii vitis-idaeae
Glycosides is object of reference.
Determination step is: phosphate buffered solution (pH6.8,30mM) 70 μ L, 1.66mM tyrosine
Solution 80 μ L, sample solution 80 μ L mix homogeneously, incubation more than 5 minutes in 37 DEG C of thermostatic water bath,
Add tyrosinase solution (125U/ml) 10 μ L, after 37 DEG C of constant temperature 10min, measure by microplate reader
The absorbance A of 475nm wavelength475.Sample aqueous solution is replaced with deionized water, same as reference solution
Measure absorbance, the results are shown in Table 2.
Inhibitory activity against tyrosinase computing formula is: suppression ratio (%)=(A0-(A475-B))/A0
× 100%, wherein A0It is the absorbance of reference solution, A475Being the absorbance of sample solution, B is sample
The absorbance of product blank solution.
The measurement result of table 2 tyrosinase inhibitory activity
From table 2, the crude extract obtained after alpine gentian is extracted has and presses down tyrosinase activity
Making use, after adsorbent resin separates, the alcohol elution of gained all can realize tyrosinase activity
Suppression, wherein alpine gentian 70% alcohol elution to tyrosinase activity than crude extract suppression make
With being greatly enhanced.
Embodiment 4
The detection of antioxidant activity
The alpine gentian crude extract prepared by embodiment 1 method and extract, with DPPH (1,1-
Diphenyl-2-trinitrophenyl-hydrazine) method measure its remove free radical antioxidant activity.Determination step is: by height
Eumeces Chinensis gall extract is configured to 0.01mg/mL aqueous solution with deionized water respectively, pipettes 2mL in 10mL
In tool plug test tube, add 2mL DPPH ethanol solution (2 × 10-4Mol/L), fully mixing, room temperature is quiet
The absorbance A of spectrophotometric determination 517nm wavelength is used after putting 30min517；Measure 2mL to carry simultaneously
Take thing solution and the mixed absorbance A of 2mL ethanol0, 2mL water mixes with 2mL DPPH ethanol solution
Absorbance C after conjunction and 2mL water and 2mL ethanol solution mixed absorbance C0.Parallel assay
Three times, averaging, calculate free radical scavenging activity according to below equation, clearance rate is the biggest, illustrates to extract
The oxidation resistance of thing is the strongest.
Free radical scavenging activity (%)=[1-(A517-A0)/(C-C0)] × 100%
The testing result of table 3 DPPH method free-radical scavenging activity
From table 3, the crude extract obtained after alpine gentian is extracted, can realize DPPH free radical
Scavenging action, after adsorbent resin separates, gained alcohol elution all can realize DPPH freedom
The removing of base, wherein alpine gentian 70% alcohol elution is to the scavenging action of free radical and crude extract phase
Relatively, improve a lot.
Embodiment 5
The alpine gentian crude extract prepared by embodiment 1 method and extract, be dissolved in deionized water,
It is configured to 1.0mg/mL aqueous solution, is sample solution, be used for measuring elastase inhibitory activity.
Assay method enters according to document (Am.J.Pharmacol.Toxicol., 2009,4,127-129) method
OK, determination step is: add 10 μ L sample solution in 96 orifice plates and 130 μ L contain 1.015mM
The 0.1M Tris-HCl buffer solution of reaction substrate Succ-Ala-Ala-Ala-p-nitroanilide
(pH8.0), incubation 5 minutes at 25 DEG C, add 15 μ L elastin laminin enzymatic solution (0.5U/mL),
Continue incubation 30min at 25 DEG C, then measure the absorbance A of 410nm wavelength by microplate reader410.With
Deionized water replaces sample aqueous solution, measures absorbance as reference solution equally, the results are shown in Table 4.
Elastase activity suppression ratio computing formula is: suppression ratio (%)=(A0-(A410-B))
/A0× 100%, wherein A0It is the absorbance of reference solution, A410Being the absorbance of sample solution, B is
The absorbance of sample blank solution.
The measurement result of table 4 elastase activity suppression
As shown in Table 4, the alpine gentian extract used in the present invention is respectively provided with suppression elastoser
Effect, wherein alpine gentian extract 70% alcohol elution suppression elastoser effect is the strongest, because of
Alpine gentian extract can be coordinated in external preparation by this, as preventing skin ageing, maintaining year spry and light
The age resister of the skin state of health uses.
Claims (10)
1. a preparation method for Radix Gentianae extract, its use in following method any one,
Method one, comprising the steps: to mix Radix Gentianae with solvent, extract, filter, removed under reduced pressure is molten
Agent,；
Method two, comprise the steps:
(1) Radix Gentianae is mixed with solvent, extract, filter, removed under reduced pressure solvent, i.e. can get Radix Gentianae
Crude extract；
(2), after Radix Gentianae crude extract being dissolved in water, it is loaded to resin packed column, successively with water and ethanol
Eluant solution, collects eluent respectively, removed under reduced pressure second alcohol and water,.
2. preparation method as claimed in claim 1, it is characterised in that described Radix Gentianae is Gentiana
(Gentiana (Tourn.) L.) plant, preferably alpine gentian (Gentiana algida Pall.), indigo plant
Flos Hostae Plantagineae Radix Gentianae (Gentiana Veitchiorum Hemsl), Changbai Mountain Radix Gentianae (Gentiana jamesii
Hemsl.), Flos Caryophylli Radix Gentianae (Gentiana szechenyii Kanitz), Dali Radix Gentianae (Gentiana taliensis
Balf.f.&Forrest) Radix Gentianae (Gentiana striolata T.N.Ho), noble Radix Gentianae (Gentiana, are spent more
Gentilis Franch), red Radix Gentianae (Gentiana rhodantha Franch.ex Hemsl.), Hemerocallis citrina Baroni Radix Gentianae
(Gentiana flavomaculata Hayata), leaf of Herba hylotelephii erythrosticti Radix Gentianae (Gentiana crassula H.Smith),
Radix Gentianae sarcorrhizae (Gentiana sarcorrhiza Ling&Ma ex T.N.Ho), Linzhi Radix Gentianae (Gentiana
Nyingchiensis T.N.Ho), fringed gentian (Gentiana panthaica Prain&Burk.), beautiful dragon
Gallbladder (Gentiana formosa H.Smith), Radix Gentianae Macrophyllae (Gentiana macrophylla Pall.), Qinghai-Tibet Radix Gentianae
(Gentiana futtereri Diels&Gilg), silk calyx Radix Gentianae (Gentiana filisepala T.N.Ho), Tianshan Mountains
Radix Gentianae Macrophyllae (Gentiana tianschanica Rupr.), Western Regions Radix Gentianae (Gentiana clarkei Kusnez.), Sillim
Radix Gentianae (Gentiana sikkimensis C.B.Clarke), Himalaya Radix Gentianae (Gentiana venusta (G.
Don) Wall.ex Griseb.), little Radix Gentianae (Gentiana parvula H.Smith), Xinjiang Radix Gentianae (Gentiana
Karelinii Griseb.), Xinjiang Radix Gentianae Macrophyllae (Gentiana walujewii Regel&Schmalh.), beautiful Radix Gentianae
(Gentiana bella Franch.ex Hemsl.), silver calyx Radix Gentianae (Gentiana albicalyx Burk.), Yushan
Radix Gentianae (Gentiana scabrida Hayata), Yunnan Radix Gentianae (Gentiana yunnanensis Franch.),
China's Radix Gentianae (Gentiana chinensis Kusnez.), Mount Everest Radix Gentianae (Gentiana stellata Turrill) and purple
One or more in flower Radix Gentianae (Gentiana syringea T.N.Ho)；It it is more preferably alpine gentian
(Gentiana algida Pall.)。
3. preparation method as claimed in claim 1, it is characterised in that method one and the step of method two
Suddenly, in (1), described be extracted as supersound extraction, stirring extraction or circulated in countercurrent are extracted；Described is super
The time that sound extracts is preferably 0.5～1.5 hour, and the time that described stirring is extracted is preferably 1.5～2
Hour, the time that described circulated in countercurrent is extracted is preferably 0.3～1 hour.
4. preparation method as claimed in claim 1, it is characterised in that method one and the step of method two
Suddenly in (1), described solvent be percent by volume be the ethanol water of 50～95%, preferably body
Long-pending percentage ratio is the ethanol water of 70%；
And/or, described Radix Gentianae is 15～30mL/g with the volume mass ratio of solvent；
And/or, described water is deionized water；
And/or, described resin is D101 resin or AB-8 resin.
5. preparation method as claimed in claim 1, it is characterised in that in the step (2) of method two,
Described ethanol solution eluting is successively with 10%～50% ethanol water, 51%～90% ethanol water
Carrying out Concentraton gradient eluting with 91%～99% ethanol water, percentage ratio is percent by volume.
6. preparation method as claimed in claim 5, it is characterised in that in the step (2) of method two,
Described ethanol solution eluting is successively with 30% ethanol water, 70% ethanol water and 95% ethanol
Aqueous solution carries out Concentraton gradient eluting；Percentage ratio is percent by volume.
7. the Radix Gentianae extract that the preparation method as according to any one of claim 1～6 prepares.
8. Radix Gentianae extract as claimed in claim 7, it is characterised in that described Radix Gentianae extract
It it is the extract that obtains of 51%～90% ethanol water eluting；Percentage ratio is percent by volume；It is preferred that
Described Radix Gentianae extract is the extract that 70% ethanol water eluting obtains；Percentage ratio is volume basis
Ratio.
9. Radix Gentianae extract as claimed in claim 7 or 8 is as tyrosinase inhibitor, elastic egg
White enzyme inhibitor, antioxidant activity agent or the application of anti-aging actives.
Apply the most as claimed in claim 9, it is characterised in that described Radix Gentianae extract is in preparation
Application in skin care item or cosmetics.
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| CN114931533B (en) * | 2022-06-27 | 2023-08-25 | 广州市中通生化制品有限公司 | Gentian multicomponent composition with multiple skin care effects and preparation method and application thereof |
| WO2024023440A1 (en) | 2022-07-26 | 2024-02-01 | Basf Beauty Care Solutions France Sas | Novel cosmetic or dermatological use of a gentiana crassicaulis extract |
| FR3138615A1 (en) | 2022-08-02 | 2024-02-09 | Basf Beauty Care Solutions France Sas | New cosmetic or dermatological use of an extract of Gentiana crassicaulis |
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