CN106265175A - Rhizoma Fagopyri Dibotryis extract, preparation method, apply and containing its skin preparations for extenal use - Google Patents
Rhizoma Fagopyri Dibotryis extract, preparation method, apply and containing its skin preparations for extenal use Download PDFInfo
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Abstract
The invention discloses Rhizoma Fagopyri Dibotryis extract, preparation method, apply and containing its skin preparations for extenal use.The invention provides the preparation method of Rhizoma Fagopyri Dibotryis extract, method 1: the mixture formed by the ethanol water that Golden Buckwheat Rhizome and volumetric concentration are 50%～80%, extracts 0.5 hour～5 hours at 10 DEG C～35 DEG C, and solid-liquid separation obtains filtrate, filtrate desolvation, obtains Rhizoma Fagopyri Dibotryis crude extract；Golden Buckwheat Rhizome is 1:8～1:15g/mL with the mass volume ratio of ethanol water；Method 2: Rhizoma Fagopyri Dibotryis crude extract method 1 obtained, is loaded to macroporous resin packed column, successively with solvent I, solvent II and solvent III eluting, collects eluent respectively, and desolvation obtains solvent eluting position A, solvent eluting position B and solvent eluting position C.The Rhizoma Fagopyri Dibotryis extract of the present invention has preferable whitening, aging resistance and antioxidative effect, has good market-oriented prospect.
Description
Technical field
The present invention relates to Rhizoma Fagopyri Dibotryis extract, preparation method, apply and containing its skin preparations for extenal use.
Background technology
Within 1956, Harman proposes free radical causes of senescence [Harman, D.Aging:A theory based
On free radical and radiation chemistry.J.Gerontol.1956,11:289-300.], it is believed that mistake
Many free radicals are the major reasons causing biological aging.Theoretical according to this, the active oxygen of internal excess
Free radical and unsaturated fatty acid effect produce the materials such as malonaldehyde, malonaldehyde by with the albumen on cell membrane
The effect such as matter produces brown pigment, is deposited in skin and becomes as various mottles.The free radical of excess can also make skin
Collagen fiber in skin, elastic fiber crosslink with degeneration, become fragile, follow the string, work as moisture of skin
Time not enough, easily make elastic fibers break, the skin ageing phenomenons such as dark stricture of vagina, microgroove, wrinkle occur.This
Outward, the ionizing radiation in environment and the environmental contaminants of such as air pollution and chemical substance etc., also can
Free radical is constantly produced in making organism.Such as ultraviolet in environment can make the fiber in dermis of skin
Blast cell and mitochondrion are upset, and then discharge superoxide anion, and the superoxide anion of excess is then
Other destructive higher free radical can be converted into.Although having in human body and being able to maintain that oxidative and anti-oxidative
Between the Antioxidative Defense System of poised state, in order to slow down the generation of active oxygen and free radical, if but
The most excessive tans by the sun in the sun, then in human body, a large amount of free radicals produced can cause the antioxidation of skin
Defence capability reduces, and causes the injury of skin, such as photoaging, creasy surface, the imbalance of skin immunization power
Deng.Antioxidant is the material having and catching free radical ability, can remove the living radical in skin,
Alleviate the skin oxidative stress damage that free radical causes, thus it is old to improve the skin caused by oxidative stress etc.
Change.The removing free radical type antioxidant being currently known in the organism such as vitamin E, vitamin C,
The most also (the patent documentations such as the antioxidant such as Flos Nelumbinis of plant origin, prunus mume (sieb.) sieb.et zucc. berry extract are reported
CN201110158540 contains Flos Nelumbinis extract and Fructus Mume extract and has antioxidation as effective ingredient
The Dermatologic preparation composition of activity).
About the formation of dermal melanin, current study show that mainly due to the bioid in melanocyte
Reaction causes, and i.e. tyrosine is under the effect as the tryrosinase of catalyst, generates DOPA quinone, then
Melanin is formed by the catalytic action of enzyme or the Oxidation of non-enzymatic.Generate about suppressing above-mentioned melanin
Whitening active thing, generally with suppression tyrosinase activity as major target class.Derive from plant extract
Tyrosinase inhibitor, as potential whitening active thing, has again safety and stimulation low to skin simultaneously
Property expection, at present the report of existing various exploitations (extract by patent documentation CN201310411195 Radix Ampelopsis
The detection method of thing white-skinned face function；CN201310382406 Lignum Aquilariae Resinatum peel extract is in preparation whitening
Application in cosmetic).
Skin collagen accounts for the 75% of dry skin weight, is the important composition composition of Skin Cell epimatrix,
Play the supporter function of skin texture, and the critical function such as tension and elastic force that skin is provided.Normal skin
In skin, generation and the decomposition of collagen are in poised state under biological regulation effect, with age, with
And the environmental factors such as ultraviolet radiation causes biological regulation miopragia, the decomposition of collagen is more than generating
During effect, the decline of collagen content in skin, skin occurs the skins such as elastic force minimizing, wrinkle generation accordingly
Catabiosis.And by the generation of suitable plant extract regulation collagen protein, or suitably increase can
Fibroblastic quantity of the collagen protein to produce, it is possible to the phenomenon of skin aging can be had by expectation
Being improved, currently reported Chinese larch sprouting, Pericarpium Citri tangerinae sprouting etc. are old from the suppression skin of plant extract
Chemical conversion point has similar improvement skin aging mechanism, and (patent documentation CN200980106591.8 contains and plants
The compositions for suppressing skin aging of the sprouting extract of thing).
Rhizoma Fagopyri Dibotryis is the root of buckwheat plant Rhizoma Fagopyri Dibotryis Fagopyrum dibotrys (D.Don) Hara
Stem, excavates winter, removes stem and fibrous root, cleans, dries and form.Rhizoma Fagopyri Dibotryis is the wildest at height above sea level
The border of 500-3000m, bushes, the other and dark and damp mountain region in Tian Bian, road.It is that Chinese Buckwheat belongs to
Wild species is distributed the widest one, on the south Daba Mountain, all has distribution to SOUTHERN CHINA in China, safe
State, India, Nepal the most generally exist.Rhizoma Fagopyri Dibotryis is China's conventional a kind of Chinese herbal medicine among the people, has
Heat-clearing and toxic substances removing, the function of evacuation of pus blood stasis dispelling, all have good therapeutical effect to lung abscess, measles pneumonia etc..
The chemical constitution study of Rhizoma Fagopyri Dibotryis starts from the Zeng Zijin such as the 1950's, foreign scholar Imai
Getting rutin in buckwheat leaf, content reaches 4～8.5%.Takahashi etc. then get one in rhizome and are referred to as
The compound of Shkauchirin, generates P-coumaric acid and ferulic acid and glucose during basic hydrolysis.In recent years,
Due to this medicine in terms for the treatment of lung abscess respond well, and there is significant antitumaous effect, cause more
Carry out the most scholars it is furtherd investigate, and achieve certain progress.Predominantly former flower
Pigment condensation property tannin mixture as (-) epicatechin, (-) epicatechin element-3-epicatechol gallate, former arrow car
Centautin B-2, B-4, and the 3 of former cyanidin B-2,3'-epicatechol gallate, flavone compound such as Cortex querci dentatae
Element, Quercitroside, luteolin etc.；Organic acid such as p-coumaric acid, ferulic acid, chlorogenic acid, coffee
Catechin sour, former, gallic acid, P-hydroxybenzoic acid etc..It is antibacterial that modern study shows that Rhizoma Fagopyri Dibotryis has
Infection, antipyretic and anti-inflammatory, eliminating phlegm and relieving cough, antiplatelet aggregation, improves the phagocytic function etc. of macrophage
Various active, in addition with stronger anti-tumor biological, it is possible to suppresses the invasion and attack of tumor cell and turns
Move.
Comprehensive Rhizoma Fagopyri Dibotryis progress in recent years, does not finds that Rhizoma Fagopyri Dibotryis is at the purposes of cosmetic field and report
Road.Find safely and effectively, defying age and the good skin preparations for extenal use of whitening effect be the skill being badly in need of at present solving
Art problem.
Summary of the invention
The technical problem to be solved is to overcome cosmetics defying age and U.S. in prior art
The defects such as white effect is poor, safety is low and provide Rhizoma Fagopyri Dibotryis extract, preparation method, apply and contain
Its skin preparations for extenal use.The Rhizoma Fagopyri Dibotryis extract free radical scavenging activity of the present invention is high, tryrosinase suppression is made
With notable, Elastase Inhibitory, notable, antioxidation and aging effect show excellent substantially, simultaneously
Good whitening, and natural, safety, can be used as whitening active ingredients and the defying age of skin preparations for extenal use
Active component.The skin preparations for extenal use composition of the present invention is natural, safety, and has whitening, antioxidation and old
The effect changed, has good market application foreground.
The invention provides the preparation method of a kind of Rhizoma Fagopyri Dibotryis extract, it uses method 1 or method 2 to enter
OK:
Described method 1 comprises the steps: that by Golden Buckwheat Rhizome and volumetric concentration be 50%～80%
The mixture that ethanol water is formed, extracts 0.5 hour～5 hours at 10 DEG C～35 DEG C, and solid-liquid divides
From obtaining filtrate, filtrate desolvation, obtain Rhizoma Fagopyri Dibotryis crude extract；Wherein, described Golden Buckwheat Rhizome with
The mass volume ratio of described ethanol water is (1:8) g/mL～(1:15) g/mL；Described
Volumetric concentration refers to the volume of ethanol and the percentage ratio of ethanol water cumulative volume.
Described method 2 comprises the steps: Rhizoma Fagopyri Dibotryis crude extract method 1 obtained, and is loaded to big
Hole resin packed column, successively with solvent I, solvent II and solvent III eluting, collects eluent respectively, and
After desolvation, it is correspondingly made available solvent eluting position A, solvent eluting position B and solvent eluting position
C, described solvent I, described solvent II and described solvent II I respectively volumetric concentration be 20%～
The ethanol water of 30%, volumetric concentration be 40%～50% ethanol water and volumetric concentration be
The ethanol water of 60%～70%.Solvent I correspondence solvent eluting position A；Solvent II correspondence solvent is washed
De-position B；Solvent II I correspondence solvent eluting position C.
In method 1, described Rhizoma Fagopyri Dibotryis (Fagopyrum dibotrys (D.Don) Hara) is Polygonaceae
(Polygonaceae) Fagopyrum (Fagopyrum) plant.
In method 1, the granularity of described Golden Buckwheat Rhizome is preferably 20 mesh～60 mesh.
In method 1, described it is extracted as the conventional method of this generic operation in this area, the most ultrasonic
Extract.The power of described supersound extraction is preferably 500w～1000w.
In method 1, the temperature of described extraction is preferably 20 DEG C～25 DEG C.
In method 1, the time of described extraction is preferably 0.5 hour～1 hour.
In method 1, the number of times of described extraction is preferably 1～2 time.
In method 1, the mass volume ratio of described Golden Buckwheat Rhizome and described ethanol water is preferably
For (1:10) g/mL～(1:15) g/mL, it is more preferably (1:10) g/mL～(1:12) g/mL.
In method 1, the volumetric concentration of described ethanol is preferably 70%.
In method 1, the method for described solid-liquid separation is the conventional method of this generic operation in this area, relatively
Goodly for filtration, filter pressing or centrifugal.
In method 1 or in method 2, the method for described filtrate desolvation is this generic operation in this area
Conventional method, preferably vacuum rotary steam.The operating condition of described vacuum rotary steam is should in this area
The normal condition of generic operation.
" filtrate desolvation " described in method 1, can be to remove whole solvents or only remove second
Alcohol.When " the filtrate desolvation " described in method 1 is for removing whole solvent, described in method 2
" the Rhizoma Fagopyri Dibotryis crude extract that method 1 is obtained, be loaded to macroporous resin packed column " need method 1
After the Rhizoma Fagopyri Dibotryis crude extract water dissolution obtained, then it is loaded to macroporous resin packed column.When institute in method 1
When " the filtrate desolvation " stated is for only removing ethanol, containing water in the Rhizoma Fagopyri Dibotryis crude extract obtained, side
" Rhizoma Fagopyri Dibotryis crude extract method 1 obtained, be loaded to macroporous resin packed column " described in method 2 just
The Rhizoma Fagopyri Dibotryis crude extract loading that can directly method 1 be obtained, without golden buckwheat method 1 obtained
After wheat crude extract water dissolution, then it is loaded to macroporous resin packed column.
In method 2, described macroporous resin packed column is the macroporous resin packed column that this area is conventional, relatively
Goodly for D101, AB-8 or ADS-8 resin packed column, it it is more preferably AB-8 resin packed column.
In method 2, described solvent I preferably volumetric concentration is the ethanol of 30%.Described solvent
II preferably volumetric concentration is the ethanol of 50%.Described solvent III preferably volumetric concentration is 70%
Ethanol.
In method 2, the consumption of described solvent I, solvent II or solvent III respectively be preferably 500mL～
800mL.The consumption of described solvent I, solvent II or solvent III can be identical or different, preferably institute
The consumption of solvent I, solvent II or the solvent III stated is identical.
In method 2, the method for described desolvation is the conventional method of this generic operation in this area, relatively
Goodly for vacuum rotary steam.The operating condition of described vacuum rotary steam is the conventional strip of this generic operation in this area
Part.
Present invention also offers the Rhizoma Fagopyri Dibotryis extract that described preparation method prepares.Described Rhizoma Fagopyri Dibotryis carries
Take thing and include the prepared Rhizoma Fagopyri Dibotryis crude extract of method 1, the prepared solvent eluting position A of method 2, method
One or more in the solvent eluting position C that the 2 solvent eluting position B prepared and method 2 prepare.
Described Rhizoma Fagopyri Dibotryis extract is preferably solvent eluting position B that method 2 prepares and method 2 prepares
Solvent eluting position C, is more preferably the prepared solvent eluting position C of method 2.
Present invention also offers the application in preparing skin preparations for extenal use of the described Rhizoma Fagopyri Dibotryis extract.
Described Rhizoma Fagopyri Dibotryis extract is lived as whitening active ingredients, aging resistance when preparing skin preparations for extenal use
One or more in property composition and oxidation-resistant active ingredient.
In the present invention, described skin preparations for extenal use includes the medicine possessing disease treatment purposes that this area is conventional
Thing, or do not possess the cosmetics of disease treatment purposes.
Present invention also offers a kind of skin preparations for extenal use including described Rhizoma Fagopyri Dibotryis extract.
Without prejudice to the field on the basis of common sense, above-mentioned each optimum condition, can combination in any, obtain this
Invent each preferred embodiments.
Agents useful for same of the present invention and raw material are the most commercially.
In the present invention, described room temperature refers to that ambient temperature is 10 DEG C～35 DEG C.
The actively progressive effect of the present invention is: the Rhizoma Fagopyri Dibotryis extract of the present invention have preferable whitening,
Aging resistance and antioxidative effect, have good market-oriented prospect.
Detailed description of the invention
Further illustrate the present invention below by the mode of embodiment, but the most therefore limit the present invention to
Among described scope of embodiments.The experimental technique of unreceipted actual conditions in the following example, according to often
Rule method and condition, or select according to catalogue.
Embodiment 1 is with ethanol water that volumetric concentration is 50% as Extraction solvent (method 1)
Rhizoma Fagopyri Dibotryis dried powder 20g (granularity of Golden Buckwheat Rhizome is 20 mesh), under room temperature (25 DEG C) with
Volumetric concentration is the ethanol water 200ml supersound extraction 2 times of 50%, each 30min (power 500W),
Taking filtrate to merge, decompression (50 DEG C) recycling design, to dry, take extract appropriate, adds DMSO (diformazan
Sulfoxide) it is configured to the solution for standby of 0.05mg/ml, measure its free radical scavenging effect, its free radical is clear
Except rate is 7%.
Embodiment 2 is with ethanol water that volumetric concentration is 60% as Extraction solvent (method 1)
Rhizoma Fagopyri Dibotryis dried powder 20g (granularity of Golden Buckwheat Rhizome is 30 mesh), under room temperature (25 DEG C) with
Volumetric concentration is the ethanol water 200ml supersound extraction 2 times of 60%, each 30min (power 500W),
Taking filtrate to merge, decompression (50 DEG C) recycling design, to dry, take extract appropriate, adds DMSO (diformazan
Sulfoxide) it is configured to the solution for standby of 0.05mg/ml, measure its free radical scavenging effect, its free radical is clear
Except rate is 23%.
Embodiment 3 is with ethanol water that volumetric concentration is 70% as Extraction solvent (method 1)
Rhizoma Fagopyri Dibotryis dried powder 20g (granularity of Golden Buckwheat Rhizome is 40 mesh), under room temperature (25 DEG C) with
Volumetric concentration is the ethanol water 200ml supersound extraction 2 times of 70%, each 30min (power 500W),
Taking filtrate to merge, decompression (50 DEG C) recycling design, to dry, take extract appropriate, adds DMSO (diformazan
Sulfoxide) it is configured to the solution for standby of 0.05mg/ml, measure its free radical scavenging effect, its free radical is clear
Except rate is 51%.
Embodiment 4 is with ethanol water that volumetric concentration is 80% as Extraction solvent (method 1)
Rhizoma Fagopyri Dibotryis dried powder 20g (granularity of Golden Buckwheat Rhizome is 50 mesh), under room temperature (25 DEG C) with
Volumetric concentration is the ethanol water 200ml supersound extraction 2 times of 80%, each 30min (power 500W),
Taking filtrate to merge, decompression (50 DEG C) recycling design, to dry, take extract appropriate, adds DMSO (diformazan
Sulfoxide) it is configured to the solution for standby of 0.05mg/ml, measure its free radical scavenging effect, its free radical is clear
Except rate is 11%.
Embodiment 5 Rhizoma Fagopyri Dibotryis is carried out for (1:8) g/mL with the mass volume ratio of described ethanol water
Extract
Rhizoma Fagopyri Dibotryis dried powder 20g (granularity of Golden Buckwheat Rhizome is 60 mesh), under room temperature (25 DEG C) with
Volumetric concentration is ethanol water 160ml, the supersound extraction 2 times of 70%, each 30min (power 500W),
Taking filtrate to merge, decompression (50 DEG C) recycling design, to dry, take extract appropriate, adds DMSO (diformazan
Sulfoxide) it is configured to 0.05mg/ml solution for standby, measure its free radical scavenging effect, its free radical scavenging
Rate is 40%.
Embodiment 6 Rhizoma Fagopyri Dibotryis is carried out for (1:10) g/mL with the mass volume ratio of described ethanol water
Extract
Rhizoma Fagopyri Dibotryis dried powder 20g (granularity of Golden Buckwheat Rhizome is 60 mesh), under room temperature (25 DEG C) with
Volumetric concentration is ethanol water 200ml, the supersound extraction 2 times of 70%, each 30min (power 500W),
Taking filtrate to merge, decompression (50 DEG C) recycling design, to dry, take extract appropriate, adds DMSO (diformazan
Sulfoxide) it is configured to 0.05mg/ml solution for standby, measure its free radical scavenging effect, its free radical scavenging
Rate is 51%.
Embodiment 7 Rhizoma Fagopyri Dibotryis is carried out for (1:12) g/mL with the mass volume ratio of described ethanol water
Extract
Rhizoma Fagopyri Dibotryis dried powder 20g (granularity of Golden Buckwheat Rhizome is 60 mesh), under room temperature (25 DEG C) with
Volumetric concentration is 70% ethanol water 240ml, supersound extraction 2 times, each 30min (500W),
Taking filtrate to merge, decompression (50 DEG C) recycling design, to dry, take extract appropriate, adds DMSO (diformazan
Sulfoxide) it is configured to 0.05mg/ml solution for standby, measure its free radical scavenging effect, its free radical scavenging
Rate is 48%.
Embodiment 8 Rhizoma Fagopyri Dibotryis is carried out for (1:15) g/mL with the mass volume ratio of described ethanol water
Extract
Rhizoma Fagopyri Dibotryis dried powder 20g (granularity of Golden Buckwheat Rhizome is 60 mesh), under room temperature (25 DEG C) with
Volumetric concentration is ethanol water 300ml, the supersound extraction 2 times of 70%, each 30min (power 500W),
Taking filtrate to merge, decompression (50 DEG C) recycling design, to dry, take extract appropriate, adds DMSO (diformazan
Sulfoxide) it is configured to 0.05mg/ml solution for standby, measure its free radical scavenging effect, its free radical scavenging
Rate is 46%.
The result of embodiment 1-8 shows: choose with ethanol solution that volumetric concentration is 70% as Extraction solvent,
Rhizoma Fagopyri Dibotryis and the mass volume ratio of ethanol water are that 1:10g/mL extraction effect is optimal.
Embodiment 9
Depletion Semen Fagopyri Esculenti dried powder 50g (granularity of Golden Buckwheat Rhizome is 60 mesh), under room temperature (25 DEG C)
With ethanol water 500ml that volumetric concentration is 70%, supersound extraction 2 times, each 30min (power
500W), filtrate merges (about 900ml), and decompression (50 DEG C) recycling design is to (the most only removing without alcohol taste
Ethanol), cross AB-8 resin, successively with volumetric concentration for ethanol water that 500ml volumetric concentration is 30%
Solution, 500ml volumetric concentration be 50% ethanol water and 500ml volumetric concentration be the ethanol of 70%
Aqueous solution eluting, collects each eluent (the most about 400ml) respectively, is evaporated, respectively obtain eluting position A
(corresponding volume concentration be 30% the eluant of ethanol water, 1.85g), position B is (right for solvent eluting
The eluant of the ethanol water answering volumetric concentration to be 50%, 1.05g) and solvent eluting position C (correspondence
Volumetric concentration is the eluant of the ethanol water of 70%, 0.78g).
Effect example 1
What this effect example was investigated is the antioxidant activity of Rhizoma Fagopyri Dibotryis extract.According to embodiment 1-9 system
Standby Rhizoma Fagopyri Dibotryis extract, measures it and removes 1, and 1-diphenyl-2-trinitrophenyl-hydrazine (DPPH) is freely
Base ability.
Determination step is: with deionized water, Rhizoma Fagopyri Dibotryis extract is configured to aqueous solution respectively, pipettes 2mL
In 10mL tool plug test tube, add 2mL DPPH ethanol solution (2 × 10-4Mol/L), fully mix,
Room temperature stands, by the absorbance A of spectrophotometric determination 517nm wavelength after 30min517；Measure simultaneously
2mL extract solution and the mixed absorbance A of 2mL ethanol0, 2mL water and 2mL DPPH ethanol
Solution mixed absorbance C and 2mL water and 2mL ethanol solution mixed absorbance C0.Flat
Row measures three times, averages, and calculates free radical scavenging activity according to below equation, and clearance rate is the biggest, says
The oxidation resistance of bright extract is the strongest.
Free radical scavenging activity (%)=[1-(A517-A0)/(C-C0)] × 100%.
The measurement result of table 1 DPPH method free radical scavenging activity
From table 1, Rhizoma Fagopyri Dibotryis extract has the ability removing the most by force free radical, through macroporous absorption tree
The ethanol water eluting position that Rhizoma Fagopyri Dibotryis volumetric concentration is 50% obtained after fat separation and Rhizoma Fagopyri Dibotryis body
Volume concentrations is that the activity of the ethanol water eluting position removing free radical of 70% is relatively strong, and Rhizoma Fagopyri Dibotryis volume is dense
Degree be 70% ethanol water eluting position the strongest.
Effect example 2
What this effect example was investigated is the Rhizoma Fagopyri Dibotryis extract inhibitory activity for tryrosinase.According to reality
Execute the Rhizoma Fagopyri Dibotryis extract of example 9 preparation, be dissolved in deionized water, be configured to aqueous solution, be used for measuring cheese ammonia
Acid enzyme inhibition activity.Determination step is: phosphate buffered solution (pH6.8,30mM) 70 μ L,
1.66mM tyrosine solution 80 μ L, sample solution 80 μ L mix homogeneously, 37 DEG C of middle temperature of thermostatic water bath
Educate 5 minutes, add tyrosinase solution (125U/mL) 10 μ L, after 37 DEG C of constant temperature 10min,
The absorbance A of 475nm wavelength is measured by microplate reader475.Replace sample aqueous solution with deionized water, make
Measure absorbance for reference solution equally, the results are shown in Table 2.
Inhibitory activity against tyrosinase computing formula is: suppression ratio (%)=(A0-(A475-B))
/A0× 100%, wherein A0It is the absorbance of reference solution, A475Being the absorbance of sample solution, B is
The absorbance of sample blank aqueous solution.
The measurement result of table 2 tyrosinase inhibitory activity
As shown in Table 2, each Rhizoma Fagopyri Dibotryis extract used in the present invention all has tyrosinase activity to be pressed down
Make and use.Wherein, the ethanol that Rhizoma Fagopyri Dibotryis volumetric concentration is 70% obtained after macroporous adsorbent resin separates
The suppression tryrosinase effect of aqueous solution eluting position is relatively strong, can be coordinated in external preparation, as anti-
Only skin Pigmented whitening active thing uses.
Effect example 3
What this effect example was investigated is the anti-aging active of Rhizoma Fagopyri Dibotryis extract.Prepare according to embodiment 9
Rhizoma Fagopyri Dibotryis extract, be dissolved in deionized water, be configured to aqueous solution, be used for measuring elastoser and press down
System activity.Assay method is according to document (Am.J.Pharmacol.Toxicol., 2009,4,127-129)
Method is carried out, and determination step is: add 10 μ L sample solution in 96 orifice plates and 130 μ L contain
The 0.1M Tris-HCl of 1.015mM reaction substrate Succ-Ala-Ala-Ala-p-nitroanilide delays
Dissolved liquid (pH8.0), incubation 5 minutes at 25 DEG C, add 15 μ L elastin laminin enzymatic solution
(0.5U/mL), continue incubation 30min at 25 DEG C, then measure 410nm wavelength by microplate reader
Absorbance A410.Replace sample aqueous solution with deionized water, measure absorbance as reference solution equally,
The results are shown in Table 3.
Elastase activity suppression ratio computing formula is: suppression ratio (%)=(A0-(A410-B))
/A0× 100%, wherein A0It is the absorbance of reference solution, A410Being the absorbance of sample solution, B is
The absorbance of sample blank solution.
The measurement result of table 3 elastase activity suppression
As shown in Table 3, the Rhizoma Fagopyri Dibotryis extract used in the present invention is respectively provided with suppression elastoser and makees
With, the ethanol water that Rhizoma Fagopyri Dibotryis volumetric concentration is 70% wherein obtained after macroporous adsorbent resin separates
The suppression elastoser effect of eluting position is the strongest, can be coordinated in external preparation, as preventing flesh
Skin is aging, maintain the age resister of the skin state of young healthy to use.
Claims (10)
1. the preparation method of a Rhizoma Fagopyri Dibotryis extract, it is characterised in that it uses method 1 or method 2
Carry out:
Described method 1 comprises the steps: that by Golden Buckwheat Rhizome and volumetric concentration be 50%～80%
The mixture that ethanol water is formed, extracts 0.5 hour～5 hours at 10 DEG C～35 DEG C, and solid-liquid divides
From obtaining filtrate, filtrate desolvation, obtain Rhizoma Fagopyri Dibotryis crude extract；Wherein, described Golden Buckwheat Rhizome with
The mass volume ratio of described ethanol water is (1:8) g/mL～(1:15) g/mL, described
Volumetric concentration refers to the volume of ethanol and the percentage ratio of ethanol water cumulative volume；
Described method 2 comprises the steps: Rhizoma Fagopyri Dibotryis crude extract method 1 obtained, and is loaded to big
Hole resin packed column, successively with solvent I, solvent II and solvent III eluting, collects eluent respectively, and
After desolvation, it is correspondingly made available solvent eluting position A, solvent eluting position B and solvent eluting position
C, described solvent I, described solvent II and described solvent II I respectively volumetric concentration be 20%～
The ethanol water of 30%, volumetric concentration be 40%～50% ethanol water and volumetric concentration be
The ethanol water of 60%～70%, solvent I correspondence solvent eluting position A；Solvent II correspondence solvent is washed
De-position B；Solvent II I correspondence solvent eluting position C.
2. the preparation method of Rhizoma Fagopyri Dibotryis extract as claimed in claim 1, it is characterised in that:
In method 1, the granularity of described Golden Buckwheat Rhizome is 20 mesh～60 mesh；
And/or,
In method 1, described is extracted as supersound extraction；
And/or,
In method 1, the temperature of described extraction is 20 DEG C～25 DEG C；
And/or,
In method 1, the time of described extraction is 0.5 hour～1 hour；
And/or,
In method 1, the number of times of described extraction is 1～2 time；
And/or,
In method 1, described Golden Buckwheat Rhizome is (1:10) with the mass volume ratio of described ethanol water
G/mL～(1:15) mg/mL；
And/or,
In method 1, the volumetric concentration of described ethanol is 70%；
And/or,
In method 1, the method for described solid-liquid separation is filtration, filter pressing or centrifugal.
3. the preparation method of Rhizoma Fagopyri Dibotryis extract as claimed in claim 2, it is characterised in that:
In method 1, the power of described supersound extraction is 500w～1000w；
And/or,
In method 1, described Golden Buckwheat Rhizome is (1:10) with the mass volume ratio of described ethanol water
G/mL～(1:12) g/mL.
4. the preparation method of Rhizoma Fagopyri Dibotryis extract as claimed in claim 1, it is characterised in that:
In method 2, described macroporous resin packed column is D101, AB-8 or ADS-8 resin packed column；
And/or,
In method 2, described solvent I is the ethanol of 30% for volumetric concentration；
And/or,
In method 2, described solvent II is the ethanol of 50% for volumetric concentration；
And/or,
In method 2, described solvent III is the ethanol of 70% for volumetric concentration；
And/or,
In method 2, the consumption of described solvent I, solvent II or solvent III is respectively 500mL～800mL.
5. the golden buckwheat that the preparation method of the Rhizoma Fagopyri Dibotryis extract as described in any one of Claims 1 to 4 prepares
Wheat extract.
6. Rhizoma Fagopyri Dibotryis extract as claimed in claim 5, it is characterised in that: described Rhizoma Fagopyri Dibotryis carries
Take thing and include the prepared Rhizoma Fagopyri Dibotryis crude extract of method 1, the prepared solvent eluting position A of method 2, method
One or more in the solvent eluting position C that the 2 solvent eluting position B prepared and method 2 prepare.
7. Rhizoma Fagopyri Dibotryis extract application in preparing skin preparations for extenal use as claimed in claim 5.
Apply the most as claimed in claim 7, it is characterised in that: described Rhizoma Fagopyri Dibotryis extract is in system
As in whitening active ingredients, anti-aging actives and oxidation-resistant active ingredient during standby skin preparations for extenal use
One or more.
Apply the most as claimed in claim 7, it is characterised in that: described skin preparations for extenal use includes tool
The medicine of standby disease treatment purposes, or do not possess the cosmetics of disease treatment purposes.
10. a skin preparations for extenal use, it is characterised in that: include the Rhizoma Fagopyri Dibotryis described in claim 5 or 6
Extract.
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| CN110710682A (en) * | 2018-07-12 | 2020-01-21 | 大江生医股份有限公司 | Application of tartary buckwheat seed coat extract in improving mitochondrial activity, promoting anti-aging gene expression and inhibiting protein glycosylation |
| CN111743937A (en) * | 2020-06-10 | 2020-10-09 | 贵州省草业研究所 | A method for preparing rhizoma Fagopyri Dibotryis extract containing rutin and luteolin as active components |
| CN113133959A (en) * | 2021-05-10 | 2021-07-20 | 江苏省中国科学院植物研究所 | Wild buckwheat rhizome extract, preparation method and application thereof |
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| CN101544703A (en) * | 2009-04-30 | 2009-09-30 | 上海大学 | Method for extracting golden buckwheat high polymeric proanthocyanidin and preparing oligomeric proanthocyanidin by carrying out catalytic degradation on same |
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| CN102397342A (en) * | 2011-11-18 | 2012-04-04 | 华颐药业有限公司 | Golden buckwheat rhizome extract, pharmaceutical preparation containing golden buckwheat rhizome extract and preparation method thereof |
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| CN101037429A (en) * | 2007-04-25 | 2007-09-19 | 上海大学 | Extraction method of Chinese medicine of gold buckwheat raw anthocyanin |
| CN101632722A (en) * | 2008-07-23 | 2010-01-27 | 曲靖开发区格力康生物科技发展有限公司 | Wild buckwheat rhizome polyphenol extract and preparation method thereof |
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| CN110710682A (en) * | 2018-07-12 | 2020-01-21 | 大江生医股份有限公司 | Application of tartary buckwheat seed coat extract in improving mitochondrial activity, promoting anti-aging gene expression and inhibiting protein glycosylation |
| CN111743937A (en) * | 2020-06-10 | 2020-10-09 | 贵州省草业研究所 | A method for preparing rhizoma Fagopyri Dibotryis extract containing rutin and luteolin as active components |
| CN113133959A (en) * | 2021-05-10 | 2021-07-20 | 江苏省中国科学院植物研究所 | Wild buckwheat rhizome extract, preparation method and application thereof |
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