KR20210026036A - Cosmetic Composition For Improving Skin Photoaging Comprising the Abeliophyllum Extract Fermented by Lactobacteria, And Manufacturing Method Thereof - Google Patents

Abstract

The present invention is to provide a cosmetic composition for alleviating skin photoaging, comprising an Abeliophyllum extract fermented by lactic acid bacteria, which is effective in antioxidation, wrinkle alleviation, skin whitening, anti-inflammatory activity, and the like in response to photoaging of the skin by ultraviolet rays, while ensuring safety for use. The cosmetic composition for alleviating skin photoaging includes an Abeliophyllum extracted liquid fermented by lactic acid bacteria as an active ingredient, wherein the Abeliophyllum extracted liquid fermented by lactic acid bacteria is fermented by inoculating 2% (v/v) of Lactobacillus plantarum and Levilactobacillus brevis, respectively, into the Abeliophyllum extract at the same time.

Description

Cosmetic Composition For Improving Skin Photoaging Comprising the Abeliophyllum Extract Fermented by Lactobacteria, And Manufacturing Method Thereof}

The present invention relates to a cosmetic composition, and in particular, to a cosmetic composition that has been effective in improving the photoaging of the skin, including the extract fermented by lactic acid bacteria, and to a method for producing the same.

The skin is largely composed of three layers: epidermis, dermis, and hypodermis, and the stratum corneum, the outermost layer of the epidermis, acts as a skin barrier, inhibiting the loss of moisture and electrolytes from the skin. The dermal layer maintains the elasticity of the skin and supports the structure through the synthesis of collagen and elastin.

On the other hand, skin aging appears as intrinsic aging that occurs naturally with age and photoaging that occurs due to exposure to accumulated external environments such as ultraviolet rays.

Repetitive exposure to UV rays increases collagen degrading enzyme and causes denaturation and destruction of collagen fibers, reducing skin elasticity and promoting wrinkle formation. As a defense against damage, melanin is produced, which moves and accumulates in the epidermis of the skin, causing pigmentation of the skin, and the overproduction and accumulation of such melanin causes freckles, melasma, erythema, aging and skin cancer. Get involved.

Therefore, various researches and developments have been made on cosmetics that can improve skin damage caused by photoaging using natural materials. For example, Korean Patent Registration No. 10-1543461 (hereinafter, "Patent Document 1" ), discloses a functional cosmetic composition for improving skin photoaging containing the extract of Yagwanmun LPC, and Korean Patent No. 10-1539688 (hereinafter referred to as "Patent Document 2") discloses a red pepper capsicum resin. Disclosed is a cosmetic material that is effective for improving skin photoaging.

On the other hand, Abeliophylli distichi Folium is a shrub called Miseon (尾扇) tree because the shape of its fruit resembles a fan, and it is a Korean specialty plant that only grows in Korea, which has only one genus in the world. Various attempts are being made to utilize it.

For example, Korean Patent Registration No. 10-1694707 (hereinafter referred to as “Patent Document 3”) discloses a deodorant composition containing the extract of Misun tree that can be used as a natural deodorant for improving noneral deodorization. Registered Patent No. 10-1729209 (hereinafter, referred to as "Patent Document 4") discloses a skin care product composition utilizing the antioxidant effect of Misun tree extract.

In addition, Korean Patent Publication No. 10-2013-0074172 (hereinafter referred to as “Patent Document 5”), Korean Patent Publication No. 10-2016-0093427 (hereinafter referred to as “Patent Document 6”) and Korean Patent No. No. 10-1848881 (hereinafter referred to as "Patent Document 7") discloses a cosmetic composition comprising a fermented Misun tree extract as an active ingredient.

Patent Document 1: Korean Patent Registration No. 10-1543461 Patent Document 2: Korean Patent Registration No. 10-1539688 Publication Patent Document 3: Korean Patent Registration No. 10-1694707 Patent Document 4: Korean Patent Registration No. 10-1729209 Patent Document 5: Korean Laid-Open Patent Publication No. 10-2013-0074172 Patent Document 6: Korean Laid-Open Patent Publication No. 10-2016-0093427 Patent Document 7: Korean Patent Registration No. 10-1848881 Publication

The present invention, in consideration of the prior art described above, comprises a lactic acid bacterium fermented Misun tree extract that is effective in sulfation, wrinkle improvement, whitening, anti-inflammatory, etc. in response to photoaging of the skin due to ultraviolet rays, while securing safety for use. It is to provide a cosmetic composition for improving skin photoaging.

In addition, the present invention is to provide a manufacturing method capable of efficiently producing a cosmetic composition for improving skin photoaging comprising a lactic acid bacterium fermented Misun tree extract.

The cosmetic composition for improving skin photoaging of the present invention contains a lactic acid bacteria fermented Miseon tree extract as an active ingredient, wherein the lactic acid bacteria fermented Miseon tree extract contains 2% (v/v) each of L. plantarum and L. brevis in the Miseon tree extract. It is characterized by being inoculated and fermented at the same time.

In addition, the cosmetic composition for improving skin photoaging of the present invention further comprises a functional natural extract and a natural preservative, the functional natural extract, lactobacillus / soybean fermentation extract, purslane extract, white willow bark extract, oregano leaf extract, cypress It includes a leaf extract and a broiler bark extract, and the natural preservative is characterized in that it comprises a Guzo tree leaf extract, a coconut palm fruit extract, a lactobacillus fermentation product, a golden extract and a licorice root extract.

In addition, the manufacturing method of the cosmetic composition for improving skin photoaging of the present invention includes the extraction step of extracting the Miseon tree extract from the leaves and stems of Miseon trees, and the extracted Miseon tree extract inoculated with L. plantarum and L. brevis to ferment Miseon. Fermentation step of fermenting with tree extract. It characterized in that it comprises a formulation step of mixing the raw material containing the fermented Miseon tree extract fermented in purified water and formulated into a gel-type or liquid-type cosmetic.

In addition, in the extraction step of the method for preparing a cosmetic composition for improving skin photoaging of the present invention, the extract of Miseon tree was refluxed at 90 to 95° C. for 10 hours by adding 10 times the weight of water to the crushed Miseon tree leaves and stems, and then filtered. And concentrated under reduced pressure to adjust the solid content to 1 or 2 Brix, and then sterilized at 121°C for 15 minutes, and in the fermentation step, the fermented rice bran extract is 3% (w/v) ), glucose 0.4% (v/v) and yeast extract 0.2% (w/v) were added to prepare a sterilized medium, and then L. plantarum and L. brevis were each added 2% (v/v) at the same time. It characterized in that the inoculated culture was fermented for 24 hours while culturing at ^{37 o C and 120 rpm.}

According to the present invention, it is possible to provide a cosmetic composition for improving skin photoaging, which is effective in sulfation, wrinkle improvement, whitening, anti-inflammatory, etc. in response to photoaging of the skin due to ultraviolet rays by including a lactic acid bacterium fermented rice plant extract, while ensuring safety. I can.

In addition, according to the present invention, by further comprising a functional natural extract consisting of Lactobacillus / fermented soy extract, purslane extract, white willow bark extract, oregano leaf extract, cypress leaf extract and broiler bark extract, the above-described lactic acid bacteria fermented Misun tree Without inhibiting the activity of the extract, it is possible to provide a cosmetic composition for improving skin photoaging that can effectively function also for skin soothing or moisturizing.

In addition, according to the present invention, instead of chemical preservatives, natural preservatives consisting of Guzo tree leaf extract, coconut palm fruit extract, lactobacillus fermentation product, golden extract and licorice root extract are further included to improve skin photoaging that is effective for antiseptic and hypoallergenic. A cosmetic composition can be provided.

1 is a graph showing the growth characteristics of complex lactic acid bacteria according to the addition ratio of Miseon tree extract
Figure 2 is a graph showing the growth characteristics of complex lactic acid bacteria according to the addition of sugar and yeast
Figure 3 is a graph showing the cell survival rate for HaCaT of Misun tree extract
Figure 4 is a graph showing the cell survival rate for HaCaT of the lactic acid bacteria fermented Misun tree extract according to the present invention
Figure 5 is a graph showing the anti-inflammatory activity of the lactic acid bacteria fermented Miseon tree extract according to the present invention

Hereinafter, specific examples of the cosmetic composition for improving skin photoaging comprising the lactic acid bacteria fermented Misun tree extract of the present invention (hereinafter, referred to as "cosmetic composition") will be described in detail.

The cosmetic composition according to the present invention contains, as an active ingredient, a lactic acid bacterium fermented rice plant extract.

In addition, the cosmetic composition according to the present invention further comprises a functional natural extract, the functional natural extract, lactobacillus / soybean fermentation extract, purslane extract, white willow bark extract, oregano leaf extract, cypress leaf extract and broiler bark Contains extracts.

In addition, the cosmetic composition according to the present invention further comprises a natural preservative, wherein the natural preservatives include Guzo tree leaf extract, coconut palm fruit extract, lactobacillus fermented product, golden extract and licorice root extract.

[Lactobacillus fermented rice plant extract]

(1) Extraction of Miseon tree extract

After washing the leaves and stems of Misen trees, drying them to about 10% moisture content, crush and cut the stem into 1 to 2 mm and the leaves to 2 to 3 cm to obtain crushed material After 10 times the weight of water was added to the pulverized rice tree leaves and stems, the mixture was extracted under reflux at 90 ~ 95℃ for 10 hours, then filtered and concentrated under reduced pressure (60℃ or less) to adjust the solid content to 1 or 2 Brix. Then, it was sterilized at 121°C for 15 minutes.

(2) Fermentation of Lactic Acid Bacteria from Sunflower Extract

① Selection of lactic acid bacteria for complex fermentation

Microbial strains may be used alone, but when two or more strains are used for fermentation at the same time, fermentation ability and palatability can be improved through interactions between the strains.

Therefore, Lactobacillus plantarum (hereinafter referred to as "L. plantarum"), Lactobacillus brevis (hereinafter referred to as "L. brevis"), Lactobacillus sakei (hereinafter referred to as "L. sakei") and other lactic acid bacteria 3 isolated from plants 3 For the species, in order to select the most suitable lactic acid bacteria for fermentation of the S. serrata, the OD value change obtained after 12 hours cultivation under the same fermentation conditions and a liquid medium containing S. S. lactic acid as the main raw material for the above three candidate lactic acid bacteria The antioxidant activity and total polyphenol content were compared and analyzed.

<Table 1>

As shown in <Table 1> above, L. plantarum and L. brevis, which are relatively excellent in growth rate, DPPH scavenging ability, and total polyphenol content, were selected as the most suitable lactic acid bacteria for fermentation of rice serrata.

② Optimization of fermentation process for fermentation of complex lactic acid bacteria of Miseon trees

In order to derive the optimum raw material ratio for the extract, distilled water is fixed to 100 ml and then sterilized by adding 1%, 2%, 3%, 4%, 5% (w/v) of dried rice extract (raw material). After preparing the cultured medium, inoculate L. plantarum and L. brevis with 2% each, a total of 4% (v/v) ^{, cultivate at 37 o} C, 120 rpm, and collect the culture solution at regular time intervals. Turbidity was measured by absorbance (A600 _nm ).

As a result, as shown in [Fig. 1], up to 1 to 3% (w/v) in the content ratio of Miseon tree extract, as the content of Miseon tree extract was increased, the growth of bacteria was also increased, but the content ratio of Miseon tree extract was From 4% (w/v), the growth rate of the microbial cells slowed down as the content of the serrata was increased.

Therefore, it is preferable to set the content ratio of the Misun tree extract to 1 to 3% (w/v), and more preferably to 3% (w/v).

Next, the content ratio of the extract of Misun tree is 1%, 2%, and 3% (w/v), and for each, glucose as a carbon source for each of 0.4% (v/v) and yeast as a nitrogen source As a result of examining the growth rate of rice seedling fermentation according to the addition of nutrients by simultaneously adding 0.2% (w/v) of yeast extract, as shown in [Fig. 2], the fermentation of simple rice seedlings extract as a substrate In comparison, it was confirmed that the growth capacity of the cells was significantly higher.

(3) Efficacy evaluation of lactic acid bacteria fermented Misun tree extract

<Preparation of sample>

First, the Miseon tree extract extracted by the method of extracting the Miseon tree extract was sterilized at 121°C for 15 minutes and then cooled to prepare. The lactic acid bacteria used for fermentation of the Miseon tree extract of the present invention include L. plantarum in a frozen state and L. plantarum in a frozen state. L. brevis was pre-cultured in MRS broth medium to make it active, and then used for sample preparation.

In addition, a sterilized medium was prepared by adding 3% (w/v), glucose 0.4% (v/v), and 0.2% (w/v) yeast extract, dried in 100 ml of distilled water. After that, a simultaneous culture method was applied in which 2% (v/v) of activated L. plantarum and L. brevis were inoculated at the same time, and the culture was ^{fermented for 24 hours while culturing at 37 o} C and 120 rpm to ferment lactic acid bacteria fermented Miseon tree extract. A sample was prepared.

<Cytotoxicity evaluation>

Cell viability assay was performed to evaluate the basal cytotoxicity of Misun trees fermented with complex lactic acid bacteria. The viability of cells was analyzed by diluting the prepared Miseon tree extract and fermented Miseon tree extract, treating the cells, and then measuring the number of cells under a microscope.

HaCaT cells, a human keratinocyte line, ^{were inoculated into 96 well plates at a concentration of 4 x 10 3} cells/well at 100 μl each. After culturing for 24 hours, the medium was removed, washed once with PBS, and then the sample was treated. Miseon tree extract and fermented misun tree extract samples were treated at concentrations of 0, 0.3, 1.5, 10, 40, and 200 μg/ml, respectively, and then cultured for 24 hours. After cell culture, 100 µl of WST-1 solution was added to each well ^{and incubated at 37 o} C for 1 hour, and absorbance was measured at 450 nm using a macroplate reader.

As a result of measuring the number of cells after culturing for 24 hours after treating the Misun tree fermented with lactic acid bacteria and the Misun tree sample extracted with hot water to a concentration of 200 μg/ml, as shown in [Fig. 3] and [Fig. 4], hot water extracted Miseon In the case of the tree extract, toxicity of less than 20% was shown up to the concentration range of 0.3µg/ml, but toxicity of 40% or more was observed in a concentration-dependent manner from the concentration range of 1.5µg/ml or more, and the number of cells decreased.

On the other hand, in the case of the fermented Miseon tree extract fermented with lactic acid bacteria, toxicity of less than 15% was observed up to the concentration range of 1.5㎍/㎖, and toxicity was observed in a concentration-dependent manner starting from the concentration of 10㎍/mL or higher.

In other words, it was found that the fermented Miseon tree extract fermented with lactic acid bacteria was somewhat superior in terms of cytotoxicity compared to the hot water extracted Miseon tree extract.

<Sulfurization effect>

The antioxidant effect of the fermented Miseon tree extract was evaluated by measuring DPPH radical scavenging activity and ABTS radical scavenging activity.

DPPH radical scavenging activity was measured by Bondet (1997) method for electron donating ability to the fermented product. After adding fermented products of various concentrations to 760 μL of 300 μM DPPH/EtOH, they were allowed to stand at 37° C. for 30 minutes, and absorbance was measured at 515 nm using a UV/Visible spectrophotometer (Berkman, USA).

In addition, ABTS radical scavenging activity was measured by the method of Roberty et al. (1999). The ABTS concentration was prepared to be 1.500 at 734 nm, and the activity was measured by the difference in absorbance from the control after reacting with the fermented product at different concentrations for 10 minutes at room temperature.

In addition, the DPPH radical scavenging activity and ABTS radical scavenging activity of the fermented Miseon tree extract were compared with the activity of ascorbic acid (L-ascorbicacid) as a control, as shown in Table 2 below.

<Table 2>

As shown in <Table 2> above, DPPH radical scavenging activity and ABTS radical scavenging activity of fermented Miseon tree extract were found to increase in a concentration-dependent manner, and as a control, at the same concentration (200㎍/㎖). For corbic acid (L-ascorbicacid), DPPH radical scavenging activity was 96.7%, and ABTS radical scavenging activity was 99.2%.

<Whitening effect>

The whitening effect of the fermented Miseon tree extract samples was evaluated by tyrosinase inhibitory activity.

Tyrosinase inhibitory activity was measured according to the method of Yagi et al. In the reaction zone, 0.2 mL of a substrate solution in which 10 mM L-DOPA was dissolved in 0.5 mL of 1/15 M sodium phosphate buffer (pH 6.8) and 0.2 mL of mushroom tyrosinase (110 U/mL) were added to a mixture of 0.1 mL of the sample solution and reacted at 25°C for 2 minutes. Then, the DOPA chrome generated in the reaction solution was measured at 475 nm. The tyrosinase inhibitory activity is expressed as the rate of decrease in absorbance of the sample solution with and without addition.

As a result of comparing the Tyrosinase inhibitory activity measured for various concentrations of fermented Miseon tree extract samples with the activity of arbutin as a control, it was shown in Table 3 below.

<Table 3>

As shown in <Table 5> above, the Tyrosinase inhibitory activity of the fermented Miseon tree extract was found to increase in a concentration-dependent manner, and also 86.7% of Tyrosinase inhibition against arbutin, the control at the same concentration (200㎍/㎖). It has been shown to have an active effect.

<Wrinkle improvement efficacy>

The anti-wrinkle effect of the fermented Misun tree extract sample was evaluated by its Elastase inhibitory activity. Elastase inhibitory activity was obtained by adding 0.1 ml of fermented rice extract samples of various concentrations + 0.05 ml of elastase (0.6 units/ml) in 50mM Tris-HCl buffer (pH 8.6) solution, followed by N-succinyl-(L-Ala)3- After reacting for 30 minutes by adding 0.1 ml of p-nitroanilide (1mg/ml) in 50mM Tris-HCl buffer (pH 8.6), the absorbance was measured at 410nm. As a result of comparing the activity of retinoleic acid as a control, the following <Table 4 Appeared as >.

<Table 4>

As shown in <Table 4> above, the Elastase inhibitory activity of the fermented Miseon tree extract was found to increase in a concentration-dependent manner, and 84.5% of Elastase for the control retinoleic acid at the same concentration (200㎍/㎖). It has been shown to have an inhibitory effect.

<Anti-inflammatory effect>

The anti-inflammatory efficacy of fermented Miseon tree extract was evaluated by the amount of NO produced by LPS (Lipopolysaccharide) treatment using Raw 264.7 cells. In other words, for the measurement of NO production, Raw 264.7 cells were dispensed into a 96-well plate at ^{a density of 1.0×10 4} cells/well, cultured for 24 hours, and then treated by concentration, and LPS was treated 1 hour after sample treatment. The supernatant incubated for 24 hours was used to measure the amount of NO produced. The cell supernatant was treated with Griess reagent (1% sulfanilamide, 0.1% N-1-naphthylenediamine dihydrochloride, and 2.5% phosphoric acid), and the absorbance was measured at 540 nm to analyze the NO production inhibitory effect. Is the same as In <Figure 5>, DX represents dexamethasone as a control material.

As a result of confirming the anti-inflammatory effect by the amount of NO produced by LPS treatment in Raw 264.7 cells, as shown in <Fig. 5>, the concentration-dependent fermented rice extract of the present invention effectively inhibits NO production, thereby exhibiting anti-inflammatory activity. In addition, the extract showed an anti-inflammatory activity of about 56% compared to the same concentration of the control substance dexamethasone.

(3) As described above, the fermented Miseon tree extract according to the present invention is effective in sulfation, whitening, wrinkle improvement and anti-inflammatory, and safety as a raw material for cosmetics has been confirmed.

[Functional natural extract]

As described above, the Miseon tree extract fermented by the fermentation process established in the present invention is safe in terms of cytotoxicity compared to the Miseon tree extract before fermentation, and the sulfation efficacy is 98% or more, and the whitening effect is 87% or more compared to the control. , Wrinkle improvement effect was over 84%, anti-inflammatory effect was over 55%.

Therefore, in the cosmetic composition for improving skin photoaging of the present invention, it is preferable to further add a functional natural extract to compensate for the insufficient functions (eg, skin soothing, moisturizing, etc.) in the fermented Miseon tree extract.

The functional natural extract may preferably include lactobacillus/bean fermentation extract, purslane extract, white willow bark extract, oregano leaf extract, cypress leaf extract and broiler bark extract.

The Lactobacillus/Soybean Ferment Extract is an extract obtained by fermenting soybeans with Lactobacillus bacteria. It is a high-molecular mucus material that has excellent moisturizing power to prevent dryness of the skin and is effective in enhancing the immunity of the skin. have.

The Purslane Extract (Portulaca Oleracea Extract) is an extract obtained by extracting and extracting outposts from summer to autumn from the annual purslane, and essential fatty acids essential for human body maintenance such as omega 3 fatty acids, tannins and saponins, beta-carotene glutin, It contains vitamins C, D, E, etc., and contains abundant natural moisturizing factors, so it is effective for skin moisturizing and soothing.

The white willow bark extract (Salix Alba (Willow) Bark Extract) is an extract extracted from the bark of white willow tree and contains tannin and salicylic acid (BHA), so it softens the dead skin cells of the skin and soothes problem skin. It has the effect of maintaining the skin's moisturizing power by preventing evaporation of moisture in the skin.

The oregano leaf extract (Origanum Vulgare Leaf Extract) is an extract extracted from the leaves of oregano, a perennial plant of the Lamiaceae family, and many vitamins and minerals such as vitamins A, C, E, K, iron, magnesium, potassium, and calcium As it contains, it has the effect of improving skin immunity by interfering with or removing the activity of substances harmful to the skin, and soothing the skin comfortably.

The Chamaecyparis Obtusa Leaf Extract is an extract extracted from the leaves of the cypress tree, and the leaves of the cypress tree contain a lot of terpenes that produce a phytoncide effect, and are effective in soothing problem skin such as skin troubles. It is effective in enhancing skin immunity.

The Cinnamomum Cassia Bark Extract is an extract extracted from the dry bark of broiler chicken (a type of cinnamon) known as cinnamon, and is effective in moisturizing and soothing the skin, and is involved in shrinking and controlling the pores of the skin. It has the effect of protecting the skin by blocking foreign substances that are harmful to the skin.

That is, in the cosmetic composition for improving skin photoaging of the present invention, by further comprising functional natural extracts such as lactobacillus/bean fermentation extract, purslane extract, white willow bark extract, oregano leaf extract, cypress leaf extract and broiler bark extract. , Skin soothing and moisturizing can also function effectively.

However, when these functional natural extracts were further included, it was examined whether the DPPH scavenging activity, ABTS radical scavenging activity, and tyrosinase inhibitory activity of the fermented Misun tree extract were inhibited by the further included functional natural extract.

At this time, the fermented Miseon tree extract is a Miseon tree extract fermented by the fermentation process of the present invention, and the functional natural extract is a commercially available Lactobacillus/bean fermentation extract, purslane extract, white willow bark extract, oregano leaf extract, Cypress leaf extract and broiler bark extract were mixed in the same ratio.

In addition, in a state in which the amount of the fermented Miseon tree extract was kept constant, the amount of the functional natural extract was varied and mixed with respect to the amount of the Miseon tree extract, and the concentration of the sample in which the fermented Miseon tree extract and functional natural extract were mixed Was measured for PH scavenging activity, ABTS radical scavenging activity, and tyrosinase inhibitory activity according to the addition ratio of the functional natural extract.

<Table 5>

As can be seen in <Table 5> above, lactobacillus/bean fermentation extract, purslane extract, white willow bark extract, oregano leaf extract, cypress leaf extract and broiler bark extract are mixed with functional natural extracts. The PH scavenging activity, ABTS radical scavenging activity, and tyrosinase inhibitory activity of the fermented Miseon tree extract are not inhibited. Rather, the PH scavenging activity, ABTS radical scavenging activity and tyrosinase inhibitory activity are increased compared to the case of using the fermented Misun tree extract alone. Appeared.

Therefore, even if the functional natural extract is further included in the Miseon tree extract, additional functions such as skin soothing or moisturizing by the functional natural extract can be expected without inhibiting the sulfation effect and wrinkle improvement effect by the Miseon tree extract. You will be able to.

[Natural material]

The cosmetic composition for improving skin photoaging according to the present invention includes a natural preservative, and the natural preservative includes Guzo tree leaf extract, coconut palm fruit extract, lactobacillus fermentation product, golden extract and licorice root extract.

The Pinus Sylvestris Leaf Extract is an extract extracted from the leaves of the Guju tree. The leaves of Guzomiaceae trees emit 10 times more bactericidal substances than other plants, which plants emit to protect their body from other microorganisms.In particular, the terpene (terpene) component of the leaves of Guzomiaceae is bacteria or fungi in the air. Kills, and prevents pests and weeds from invading plants.

The coconut palm extract (Cocos Nucifera (Coconut) Fruit Extract) is an extract extracted from the fruit of a coconut palm, a plant in tropical and subtropical regions. The panthenol component of the coconut palm fruit moisturizes, and in particular, the cellulose component is known to have antibacterial and wound healing effects.

The Lactobacillus Ferment is a fermented product fermented by Lactobacillus, an anaerobic microorganism that generates energy by fermenting sugars, and is known to have antibacterial and antiseptic activities.

The golden extract (Scutellaria Baicalensis Root Extract) is an ingredient extracted from the root of Scutellaria baicalensis GEORGE, a perennial herbaceous plant belonging to the Lamiaceae family, and the active ingredient baicaline helps to moisturize and prevent oxidation of the skin. It is effective in whitening by inhibiting a component called tyrosinase, which is a melanin pigment fish enzyme, and is effective in whitening. The main components of the golden extract include baicalin, baicalein, and wogonin, and in particular, the high antibacterial activity of baicalin is known.

The licorice root extract (Glycyrrhiza Uralensis (Licorice) Root Extract) is an extract of licorice root belonging to a legume plant. Licorice is a medicinal plant widely used as a medicinal herb in Korea, China and Japan, and the most important active ingredient of licorice is glycyrrhizin, a triterpenoid saponin, which is contained in the roots of licorice by 3-5%, and has anti-allergic, antioxidant properties, and It has physiological activities such as anti-ulcer and anti-cancer properties, and flavonoids such as liquiritigenin and liquiritin, which are trace components, are known to have antibacterial effects.

In the present invention, the antiseptic power of the natural preservatives against bacteria, including the Guzo tree leaf extract, coconut palm fruit extract, lactobacillus fermented product, golden extract and licorice root extract, was evaluated.

In the evaluation of the antiseptic ability of the natural preservative against bacteria, Staphylococcus aureus as a bacterium in a sample containing the Guju tree leaf extract, coconut palm fruit extract, lactobacillus fermentation product, golden extract and licorice root extract in the same mass ratio. (Staphylococcus aureus), Escherichia coli, and Pseudomonas aeruginosa 3 kinds of mixed bacteria ^{were inoculated to be 1.6×10 6} per 1 g of sample, and 1 day from the day of inoculation, 3 The number of viable cells was measured by taking 1 g of each cosmetic at intervals of days, 5, 7, 14, 21, and 28 days, and the results are shown in <Table 6> below.

<Table 6>

For antiseptic efficacy, bacteria should decrease the number of bacteria by at least 99.9% within 7 days of inoculation, and there should be no growth during the test period.

In the case of the natural preservative according to the embodiment of the present invention, as shown in <Table 6> above, the number of viable cells decreased by 99.9% or more within 5 days from the date of inoculation of bacteria, and no future proliferation occurred. It was confirmed that it was shown.

[Composition of cosmetics]

The cosmetic composition of the present invention comprises 60 to 70 parts by weight of purified water, 0.3 to 3 parts by weight of fermented rice plant extract, 0.02 to 3 parts by weight of functional natural extract, and 0.3 to 3 parts by weight of natural antiseptic, gel type or liquid type It can be formulated as.

In addition, the cosmetic composition of the present invention may further contain additives such as emulsifiers, thickeners, and fragrances.

Claims (4)

Contains lactic acid bacteria fermented Miseon tree extract as an active ingredient,
The lactic acid bacteria fermented Miseonnamu extract is a cosmetic composition for improving skin photoaging, characterized in that inoculation of 2% (v/v) each of L. plantarum and L. brevis to Miseonnamu extract and fermenting them at the same time.
The method of claim 1,
The cosmetic composition for improving skin photoaging further comprises a functional natural extract and a natural preservative,
The functional natural extract includes lactobacillus/bean fermentation extract, purslane extract, white willow bark extract, oregano leaf extract, cypress leaf extract and broiler bark extract,
The natural preservative is a cosmetic composition for improving skin photoaging, characterized in that it comprises a Guzo tree leaf extract, a coconut palm fruit extract, a lactobacillus fermented product, a golden extract and a licorice root extract.
An extraction step of extracting the Misun tree extract from Misun tree leaves and stems,
A fermentation step of inoculating L. plantarum and L. brevis in the extracted Miseon tree extract medium to ferment it with fermented Miseon tree extract.
A method for producing a cosmetic composition for improving skin photoaging, comprising a formulation step of mixing a raw material containing the fermented rice seed extract fermented in purified water to form a gel-type or liquid-type cosmetic. The method of claim 3,
In the extraction step, the rice serrata extract is refluxed at 90 to 95°C for 10 hours by adding 10 times the weight of water to the crushed rice stalks and stems, then filtered and concentrated under reduced pressure to have a solid content of 1 or 2 Brix. And then sterilized at 121°C for 15 minutes,
In the fermentation step, the fermented Miseon tree extract was prepared by adding the Miseon tree extract 3% (w/v), glucose 0.4% (v/v) and yeast extract 0.2% (w/v) to prepare a sterilized medium. Thereafter, a method for producing a cosmetic composition for improving skin photoaging, characterized in that the culture solution obtained by simultaneously inoculating 2% (v/v) of L. plantarum and L. brevis was ^{fermented for 24 hours while culturing at 37 o C and 120 rpm.} .

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