WO2023282427A1 - Cosmetic composition including silkworm cocoon-derived sericin and aloe barbadensis leaf extract - Google Patents
Cosmetic composition including silkworm cocoon-derived sericin and aloe barbadensis leaf extract Download PDFInfo
- Publication number
- WO2023282427A1 WO2023282427A1 PCT/KR2022/002970 KR2022002970W WO2023282427A1 WO 2023282427 A1 WO2023282427 A1 WO 2023282427A1 KR 2022002970 W KR2022002970 W KR 2022002970W WO 2023282427 A1 WO2023282427 A1 WO 2023282427A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- extract
- skin
- sericin
- cosmetic composition
- moisturizing
- Prior art date
Links
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Classifications
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- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/987—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Definitions
- the present invention relates to a cosmetic composition comprising silkworm cocoon-derived sericin and an aloe vera leaf extract, and particularly to a cosmetic composition for skin moisturizing, skin soothing, whitening and wrinkle improvement.
- Sericin is a protein constituting silkworm cocoon fibers together with fibroin, and its amino acid composition is characterized by a polar amino acid ratio such as Ser, Asp, and Thr occupying more than 60%.
- This amino acid composition is similar to the protein distribution constituting human skin, and in fact, sericin is known to have various skin effects such as whitening, anti-inflammatory, and collagen synthesis ability.
- golden silkworm cocoon is a yellow cocoon built by "Golden Silk", a breed cultivated by crossing Japanese silkworm 311 (JS169) and Chinese silkworm 312 (CS186) by the Korean Rural Development Administration from 2005 to 2007. It is known to contain more sericin than cocoons.
- Korean Patent Publication No. 10-2017-0065959 (published on June 14, 2017) contains mangosteen extract and sericin extracted from golden silkworm cocoons. It discloses a cosmetic composition for a mask pack that can delay skin damage and aging and alleviate the symptoms of atopic dermatitis, and Korean Patent Registration No. 10-2035590 (registered on October 17, 2019) is a combination of ginsenoside F5 and gold Disclosed is a cosmetic composition capable of protecting the scalp and improving hair condition, including golden sericin derived from silkworm cocoons.
- aloe vera (scientific name: Aloe barbadensis) is a succulent perennial herb that resembles a cactus in appearance, but actually belongs to the aloe genus of the lily family. .
- the leaves of aloe vera are long, lancet-shaped, pointed at the end, with slightly sharp thorns on the edge of the leaf, fleshy, without petioles, and staggered from the stem. It is known to have an excellent effect on skin moisturizing.
- Korean Patent Registration No. 10-2040245 (registered on October 29, 2019) contains an extract extracted by adding mineral water to aloe vera leaves to moisturize the skin or prevent skin aging. It discloses a cosmetic composition effective for.
- the present inventors focused on the fact that a cosmetic composition effective for skin moisturizing, whitening and wrinkle improvement can be developed by mixing sericin effective for skin whitening and wrinkle improvement and aloe vera leaf extract effective for skin moisturizing, and arrived at the present invention.
- the activity of each of the mixed natural extracts is not necessarily increased in a complementary manner. That is, when a plurality of natural extracts are mixed, a problem of mutually inhibiting the activity of the functions of each natural extract may occur.
- the present invention is to provide a cosmetic composition having effects such as moisturizing, skin soothing, whitening, and wrinkle improvement by mixing cocoon-derived sericin and aloe vera leaf extract.
- the present invention provides a cosmetic composition capable of enhancing moisturizing and skin soothing functions and advantageously supplying raw materials by adding a natural extract for moisturizing and skin soothing to the cosmetic composition to enhance the moisturizing and skin soothing functions. is to provide
- the present invention is to provide a cosmetic composition that can replace existing chemical preservatives and facilitate the supply and demand of raw materials by mixing a natural extract for preservative consisting of a plurality of natural extracts with the cosmetic composition.
- the cosmetic composition according to the present invention includes cocoon-derived Sericin and Aloe Barbadensis Leaf Extract, and is characterized in that it exhibits skin moisturizing, whitening and wrinkle improvement effects.
- the sericin of the cosmetic composition according to the present invention is characterized in that it is extracted from the golden silkworm cocoon.
- the cosmetic composition according to the present invention further comprises a natural extract for soothing and moisturizing the skin, wherein the natural extract for soothing and moisturizing the skin is made of lactobacillus/fermented soybean extract, white willow bark extract and purslane extract. to be
- the cosmetic composition according to the present invention further includes a natural extract for preservatives, and the natural extract for preservatives is characterized in that it consists of cinnamon bark extract, oregano leaf extract, cypress leaf extract and golden extract.
- the cosmetic composition according to the present invention based on the total weight, the sericin is 0.01 to 3 parts by weight, the aloe vera leaf extract is 0.1 to 3 parts by weight, the moisturizing and skin soothing natural extract is 0.02 to 3 parts by weight, the It is characterized by containing 0.02 to 5 parts by weight of natural extracts for preservatives, respectively.
- cocoon-derived sericin and aloe vera leaf extract by mixing cocoon-derived sericin and aloe vera leaf extract, there are effects such as moisturizing and skin soothing, whitening, and wrinkle improvement.
- the present invention can strengthen the moisturizing and skin soothing function and facilitate the supply and demand of raw materials by further adding a natural extract for moisturizing and skin soothing.
- the present invention can replace existing chemical preservatives by mixing natural extracts for preservatives made of a plurality of natural extracts, and can advantageously supply and demand raw materials.
- the cosmetic composition according to the present invention includes Sericin derived from a silkworm cocoon and Aloe Barbadensis Leaf Extract.
- the sericin can be extracted from silkworm cocoons or golden silkworm cocoons by refining at 80 to 120 ° C. for 1 to 3 hours in a completely immersed state with a water ratio of cocoon raw sand and distilled water of 1:15 to 25.
- sericin extracted from silkworm cocoons is a high molecular substance and has low solubility in water
- Alcalase enzyme, flavozyme enzyme, and protamex enzyme are each 1.5% with respect to the extracted sericin.
- Sericin was hydrolyzed at 60 ° C. for 36 h by adding at a (w / w) ratio, and after the reaction was completely completed, it was cut off for 1 hour to inactivate the enzyme.
- the sericin was prepared as a 2% solution using distilled water, and then the 2% solution was centrifuged (4000 rpm / 0.5 hr) to separate the supernatant Then, using a membrane filter (76mm disk filter 10Kda, 1.0Kda /Millipore), fractionation was filtered while stirring under a filtration pressure of 1.0 to 2.0 bar (N 2 gas), and powdered through freeze-drying.
- a membrane filter 76mm disk filter 10Kda, 1.0Kda /Millipore
- sericin extracted from common silkworm cocoons or golden silkworm cocoons may be preferably used, and sericin extracted from golden silkworm cocoons is more preferred.
- the aloe vera leaf extract after sufficiently washing the adult aloe vera leaves, 200 g of dried and cut aloe vera leaves were put into 2 L of methanol and extracted three times at room temperature for 7 days, and the extracted aloe vera The leaf extract was filtered and then concentrated under reduced pressure at 45° C. using a vacuum rotary evaporator to obtain an extracted powder.
- the cosmetic composition according to the present invention further includes a natural extract for soothing and moisturizing the skin
- a natural extract for soothing and moisturizing the skin consists of a lactobacillus/fermented soybean extract, a white willow bark extract, and a purslane extract.
- the Lactobacillus/Soybean Ferment Extract is an extract of a product obtained by fermenting soybeans with Lactobacillus, and the polymeric mucus material produced from fermented soybeans has excellent moisturizing power when applied to the skin. It is known to help moisturize the skin.
- the white willow bark extract (Salix Alba (Willow) Bark Extract) is an extract extracted from the bark of the white willow tree and contains tannin and salicylic acid (BHA), which gently softens the dead skin cells of the skin to soothe problem skin, It is known to have the effect of preventing the evaporation of moisture in the skin and maintaining the moisturizing power of the skin.
- the purslane extract (Portulaca Oleracea Extract) is an extract of purslane, also called portulaca oleracea or five elements, and is known to contain omega 3, tannin, saponin, beta-carotene glotin, vitamins, etc., and to help soothe skin and relieve irritation. there is.
- the cosmetic composition according to the present invention further includes a natural extract for preservative use, and the natural extract for preservative is composed of cinnamon bark extract, oregano leaf extract, cypress leaf extract, and golden extract.
- the Cinnamomum Cassia Bark Extract is an extract extracted from the dried bark of a cinnamon tree belonging to the Lauraceae family, and contains cinnamic aldehyde, camphene, cineol, linalool, Eugenol and the like are known to have antibacterial and other effects.
- the oregano leaf extract (Origanum Vulgare Leaf Extract) is an extract extracted from the mouth of oregano, contains a lot of phenolic compounds, especially rosmarinic acid, and is known to have anti-inflammatory, antioxidant, antibacterial, antifungal and antiviral properties
- the cypress tree leaf extract (Chamaecyparis Obtusa Leaf Extract) is an extract extracted from the leaves of a cypress tree, also called cypress and hinoki, and is known to contain a natural antibacterial substance called phytoncide.
- the golden extract (Scutellaria Baicalensis Root Extract) is an extract of peeled and dried roots of golden grass, a perennial herb, and contains various kinds of flavonoids and is known to have antibacterial and anti-inflammatory effects.
- the cosmetic composition contains 0.02 to 3% by weight of the sericin, 0.02 to 3% by weight of the natural extract for moisturizing and skin soothing, and 0.2 to 8% by weight of the natural extract for preservatives, based on the total weight of the cosmetic composition.
- Example 1 Preparation of mixed samples of sericin and aloe vera leaf extract
- Sericin was extracted from the golden cocoon by refining at 120 ° C for 3 hours in a state where the golden silkworm cocoon was completely immersed in a water ratio of 1:15 to distilled water, and then, Alcalase enzyme, flavozyme enzyme, and Protamex Sericin was hydrolyzed at 60 ° C. for 36 h by adding enzymes at a rate of 1.5% (w / w), and after the reaction was completely finished, it was cut off for 1 hour to inactivate the enzyme.
- the hydrolyzed sericin peptide powder was prepared as a 2% solution using distilled water. /Millipore), fractionation was filtered while stirring at a filtration pressure of 1.0 to 2.0 bar (N 2 gas), and powdered through freeze-drying.
- a sample (hereinafter referred to as "mixed sample") was prepared by mixing the sericin powder and the aloe vera leaf extract powder obtained by ⁇ 1-1> and ⁇ 1-2> in the same mass ratio.
- Test Example 1 Antioxidant effect of mixed sample
- the antioxidant effect of the mixed sample was evaluated by measuring DPPH radical scavenging activity and ABTS radical scavenging activity.
- DPPH is used to measure antioxidant activity by the principle of discoloration from purple to yellow as anion radicals are scavenged by reacting with antioxidants.
- ABTS radical scavenging activity is a method widely used to measure antioxidant activity along with the method using DPPH. ABTS reacts with antioxidants to scavenge cation radicals and is used to measure antioxidant activity on the principle of discoloration from bluish green to colorless. .
- the DPPH radical scavenging activity and the electron donating ability of the mixed sample were measured by the Bondet (1997) method.
- Mixed samples of various concentrations were added to 760 ⁇ l of 300 ⁇ M DPPH/EtOH, left at 37° C. for 30 minutes, and absorbance was measured at 515 nm using a UV/Visible spectrophotometer (Berkman, USA). Activity was measured by the difference between
- ABTS radical scavenging activity was measured by the method of Roberty et al. (1999).
- the ABTS concentration was prepared to be 1.500 at 734 nm, and the activity was measured by the difference in absorbance with the control after reacting with the mixed sample with different concentrations at room temperature for 10 minutes.
- the whitening effect of the mixed samples was evaluated by the inhibitory activity of tyrosinase, an oxidizing enzyme that regulates the production of melanin.
- Tyrosinase inhibitory activity was measured according to the method of Yagi et al. In the reaction zone, 0.2mL of mushroom tyrosinase (110U/mL) was added to a mixture of 0.2mL of substrate solution in which 10mM L-DOPA was dissolved in 0.5mL of 1/15M sodium phosphate buffer (pH 6.8) and 0.1mL of sample solution, followed by reaction at 25°C for 2 minutes. The DOPA chrome generated in the reaction solution was measured at 475 nm, and the Tyrosinase inhibitory activity is expressed as the absorbance decrease rate of the sample solution added and unadded.
- Elastase inhibitory activity was measured by adding 0.05 ml of elastase (0.6 units/ml) in 50 mM Tris-HCl buffer (pH 8.6) solution to 0.1 ml of a mixed sample of various concentrations, and then measuring N-succinyl-(L-Ala)3-p-nitroanilide ( 1mg/ml) in 50mM Tris-HCl buffer (pH 8.6) 0.1ml was added, reacted for 30 minutes, and absorbance was measured at 410nm. As a result of comparison with the activity of retinoic acid as a control, as shown in [Table 3] below appear.
- the Elastase inhibitory activity of the hop sample was found to increase in a concentration-dependent manner, and at the same concentration (200 ⁇ g/ml), 88.9% of the Elastase inhibitory activity against the control retinoic acid has been shown to be effective.
- soybean extract was obtained by secondary filtration, and the soybean extract was sterilized and lyophilized to powder.
- the fermented Lactobacillus/soybean fermented extract was firstly filtered by centrifugal separation and a filter of 0.4 to 0.5 ⁇ m level, and after removing cultured bacteria and debris, it was secondarily filtered using a filtration membrane of 0.3 ⁇ m or less, and freeze-dried. It was then powdered.
- Samples were prepared by mixing each of the lactobacillus/soybean fermented extract, white willow bark extract, and purslane extract obtained by ⁇ 2-1> and ⁇ 2-2> in the same mass ratio.
- Test Example 4 Activity inhibition of mixed samples of natural extracts for skin soothing and moisturizing
- the additional ratio (mass ratio) of the natural extract sample for skin soothing and moisturizing is different from the amount of the mixed sample, so that the natural extract sample for soothing and moisturizing the skin was mixed, and the concentration of the sample mixed with the mixed sample and the natural extract sample for skin soothing and moisturizing was 200 ⁇ g / ml, DPPH radical scavenging activity according to the additional ratio of the natural extract for skin soothing and moisturizing, ABTS Radical scavenging activity, tyrosinase inhibitory activity, and elastase inhibitory activity were measured.
- Test Example 5 Skin moisturizing effect of natural extracts for skin soothing and moisturizing
- test subjects 10 adult women who felt their skin was dry, and the natural extract for soothing and moisturizing the skin (concentration: 200 After application of ⁇ g/ml), the change in skin moisture level over time was measured and evaluated with a Corneometer (CM 825).
- the back of the hand was divided into an applied area and a non-application area, and immediately before, immediately after application, and after 30 minutes after application of the natural extract sample for skin soothing and moisturizing on the application area, 60 After lapse of minutes, after 90 minutes, and after 120 minutes, the skin moisture level at the application site was measured, and at the same time, the skin moisture level at the application site was measured and the skin moisture level at the non-application site was also measured.
- [Table 5] below is the average value of 10 test subjects, and the unit is A.U. (arbitary unit) of the manufacturer.
- Natural extract samples for preservatives were prepared by mixing the cinnamon bark extract, oregano leaf extract, cypress leaf extract, and golden extract in the same mass ratio.
- Test Example 6 Preservative effect of each natural extract and natural extract for preservative
- Staphylococcus aureus In the evaluation of the preservative power of each sample (Samples 1 to 5), Staphylococcus aureus, Escherichia coli, and Paeudomonas aeruginosa were 3 bacteria in each sample. Inoculate the mixed bacterial solution of eggplant so that 1.6X10 6 per 1g of each sample is inoculated, and take 1g of each sample at intervals of 1st, 3rd, 5th, 7th, 14th, 21st and 28th days from the date of inoculation The number of viable cells was measured, and the results are shown in [Table 5] below.
- the number of bacteria should be reduced by 99.9% or more within 7 days of inoculation, and there should be no proliferation during the test period.
- Sample type inoculum count Viable cell count over time (unit/g) 1 day 3 days 5 days 7 days 14 days 21st 28 days
- Sample 1 1.6X10 6 5.6X10 4 4.7X10 3 ⁇ 10 ⁇ 10 ⁇ 10 ⁇ 10 sample 2
- 1.6X10 6 5.3X10 4 3.6X10 3 ⁇ 10 ⁇ 10 ⁇ 10 ⁇ 10 sample 3
- 1.6X10 6 3.6X10 4 2.8X10 3 ⁇ 10 ⁇ 10 ⁇ 10 ⁇ 10 ⁇ 10 sample 4
- 1.6X10 6 4.3X10 4 2.1X10 3 ⁇ 10 ⁇ 10 ⁇ 10 ⁇ 10 ⁇ 10 sample 5
- 1.6X10 6 3.1X10 4 1.9X10 3 ⁇ 10 ⁇ 10 ⁇ 10 ⁇ 10 ⁇ 10 ⁇ 10 ⁇ 10 ⁇ 10 ⁇ 10 ⁇ 10 ⁇ 10
- the present invention is a cosmetic composition containing cocoon-derived sericin and aloe vera leaf extract, which is effective for skin moisturizing and soothing, whitening, and wrinkle improvement, and has industrial applicability.
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Abstract
The present invention is characterized by comprising silkworm cocoon-derived sericin and an Aloe barbadensis leaf extract and exhibiting skin moisturization, skin whitening, and wrinkle reduction effects.
Description
본 발명은 누에고치 유래 세리신과 알로에베라잎추출물을 포함하는 화장료 조성물에 관한 것으로, 특히 피부보습, 피부진정, 미백 및 주름개선용 화장료 조성물에 관한 것이다.The present invention relates to a cosmetic composition comprising silkworm cocoon-derived sericin and an aloe vera leaf extract, and particularly to a cosmetic composition for skin moisturizing, skin soothing, whitening and wrinkle improvement.
최근 천연물의 추출물을 함유하는 화장료에 대한 관심이 증대되어 왔으며, 이러한 천연물 중의 하나로서 누에고치로부터 추출된 세리신(Sericin)이 화장료의 원료로 사용되고 있다.Recently, interest in cosmetics containing extracts of natural substances has increased, and as one of these natural substances, sericin extracted from silkworm cocoons is used as a raw material for cosmetics.
세리신은 피브로인과 함께 누에고치 섬유를 구성하는 단백질로서, 아미노산 조성은 Ser, Asp, Thr 등의 극성 아미노산 비율이 60% 이상을 차지하는 것이 특징이다. 이러한 아미노산 구성은 인체 피부를 구성하는 단백질 분포와 유사하며 실제로 세리신은 미백, 항염, 콜라겐 합성능 등의 다양한 피부 효능을 가진다고 알려져 있다. Sericin is a protein constituting silkworm cocoon fibers together with fibroin, and its amino acid composition is characterized by a polar amino acid ratio such as Ser, Asp, and Thr occupying more than 60%. This amino acid composition is similar to the protein distribution constituting human skin, and in fact, sericin is known to have various skin effects such as whitening, anti-inflammatory, and collagen synthesis ability.
또한, 황금누에고치는 한국 농촌진흥청이 2005년부터 2007년까지 일본종계 잠311(JS169)과 중국종계 잠312(CS186)을 교잡하여 육성한 품종인 "골든실크"에 의해 지어진 황색 고치로서, 일반 누에고치보다 세리신을 더 많이 포함하고 있는 것으로 알려져 있다. In addition, the golden silkworm cocoon is a yellow cocoon built by "Golden Silk", a breed cultivated by crossing Japanese silkworm 311 (JS169) and Chinese silkworm 312 (CS186) by the Korean Rural Development Administration from 2005 to 2007. It is known to contain more sericin than cocoons.
따라서, 최근에는 일반누에고치 또는 황금누에고치로부터 추출된 세리신을 포함하는 다양한 화장료가 개발되고 있는데, 예를 들면, 한국공개특허 제10-2021-0033399호(2021.03.26. 공개)는 고분자 세리신을 용해시켜 발림성 등의 사용감을 향상시킨 화장료 조성물에 대해 개시하고 있으며, 한국등록특허 제10-1704617호(2017.02.02. 등록)는 황금누에고치를 가수분해하여 얻어진 세리신 펩타이드를 페길레이션하여 제조된 화합물을 유효성분으로 함유하여 우수한 미백 효과를 발휘하는 피부미백용 화장료 조성물을 개시하고 있다.Therefore, recently, various cosmetics containing sericin extracted from common silkworm cocoons or golden silkworm cocoons have been developed. It discloses a cosmetic composition that improves the feeling of use by dissolving, and Korean Patent Registration No. 10-1704617 (registered on February 2, 2017) is a compound prepared by pegylating sericin peptides obtained by hydrolyzing golden silkworm cocoons Disclosed is a cosmetic composition for skin whitening that contains as an active ingredient and exhibits an excellent whitening effect.
또한, 세리신을 다른 천연추출물과 혼합한 화장료도 개발되고 있는데, 예를 들면, 한국공개특허 제10-2017-0065959호(2017.06.14. 공개)는 망고스틴 추출물과 황금누에고치에서 추출한 세리신을 포함하여 피부의 손상 및 노화를 지연시키고, 아토피성 피부염의 증상을 완화시킬수 있는 마스크 팩용 화장료 조성물을 개시하고 있고, 한국등록특허 제10-2035590호(2019.10.17. 등록)는 진세노사이드 Ｆ5와 황금누에고치 유래의 황금세리신을 포함하여 두피를 보호하고 모발 상태를 개선할 수 있는 화장료 조성물을 개시하고 있다.In addition, cosmetics in which sericin is mixed with other natural extracts are being developed. For example, Korean Patent Publication No. 10-2017-0065959 (published on June 14, 2017) contains mangosteen extract and sericin extracted from golden silkworm cocoons. It discloses a cosmetic composition for a mask pack that can delay skin damage and aging and alleviate the symptoms of atopic dermatitis, and Korean Patent Registration No. 10-2035590 (registered on October 17, 2019) is a combination of ginsenoside F5 and gold Disclosed is a cosmetic composition capable of protecting the scalp and improving hair condition, including golden sericin derived from silkworm cocoons.
한편, 알로에베라(aloe vera, 학명: Aloe barbadensis)는 다즙(多汁)의 다년생 초본(草本)으로 외형상 선인장과 비슷하나 실제로는 백합과(lily family) 알로에속(aloe genus)에 속하는 식물이다. 알로에베라의 잎은 긴 바소꼴로 끝이 뾰족하고, 잎 가장자리에 약간 날카로운 가시가 있으며, 다육질로 잎자루는 없고 줄기에서 어긋나 있으며, 알로에베라잎의 다당체는 면역기능 강화 역할과 거칠고 지친 피부를 부드럽게 하고, 피부 보습에 우수한 효과가 있는 것으로 알려져 있는데, 예를 들면, 한국등록특허 제10-2040245호(2019.10.29. 등록)는 알로에베라잎에 광천수를 첨가하여 추출한 추출물을 함유하여 피부보습 또는 피부노화방지에 효과가 있는 화장료 조성물에 대해 개시하고 있다.On the other hand, aloe vera (scientific name: Aloe barbadensis) is a succulent perennial herb that resembles a cactus in appearance, but actually belongs to the aloe genus of the lily family. . The leaves of aloe vera are long, lancet-shaped, pointed at the end, with slightly sharp thorns on the edge of the leaf, fleshy, without petioles, and staggered from the stem. It is known to have an excellent effect on skin moisturizing. For example, Korean Patent Registration No. 10-2040245 (registered on October 29, 2019) contains an extract extracted by adding mineral water to aloe vera leaves to moisturize the skin or prevent skin aging. It discloses a cosmetic composition effective for.
따라서, 본 발명자는 피부 미백 및 주름개선에 효과적인 세리신과 피부보습에 효과적인 알로에베라잎추출물을 혼합함으로써, 피부보습, 미백 및 주름개선 등에 효과적인 화장료 조성물을 개발할 수 있다는 점에 착안하여 본 발명에 이르렀다.Therefore, the present inventors focused on the fact that a cosmetic composition effective for skin moisturizing, whitening and wrinkle improvement can be developed by mixing sericin effective for skin whitening and wrinkle improvement and aloe vera leaf extract effective for skin moisturizing, and arrived at the present invention.
다만, 복수의 천연추출물을 혼합하는 경우, 혼합된 천연추출물들 각각이 가지는 활성을 반드시 상보적으로 상승시키는 것은 아니다. 즉, 복수의 천연추출물을 혼합하는 경우, 각각의 천연추출물이 가지는 기능의 활성을 오히려 상호간에 저해하는 문제가 발생할 수도 있다. However, when a plurality of natural extracts are mixed, the activity of each of the mixed natural extracts is not necessarily increased in a complementary manner. That is, when a plurality of natural extracts are mixed, a problem of mutually inhibiting the activity of the functions of each natural extract may occur.
본 발명은 누에고치 유래의 세리신과 알로에베라잎추출물을 혼합하여, 보습 및 피부진정, 미백, 주름개선 등의 효과가 있는 화장료 조성물을 제공하기 위한 것이다.The present invention is to provide a cosmetic composition having effects such as moisturizing, skin soothing, whitening, and wrinkle improvement by mixing cocoon-derived sericin and aloe vera leaf extract.
또한, 본 발명은 상기 화장료 조성물에 보습 및 피부진정 기능을 강화할 수 있는 보습 및 피부진정용 천연추출물을 더 추가함으로써, 보습 및 피부진정 기능을 강화하고 원료의 수급을 유리하게 할 수 있는 화장료 조성물을 제공하기 위한 것이다.In addition, the present invention provides a cosmetic composition capable of enhancing moisturizing and skin soothing functions and advantageously supplying raw materials by adding a natural extract for moisturizing and skin soothing to the cosmetic composition to enhance the moisturizing and skin soothing functions. is to provide
또한, 본 발명은 상기 화장료 조성물에 복수의 천연추출물로 이루어진 방부용 천연추출물을 혼합함으로써, 기존의 화학적 방부제를 대체할 수 있고, 원료의 수급을 유리하게 할 수 있는 화장료 조성물을 제공하기 위한 것이다.In addition, the present invention is to provide a cosmetic composition that can replace existing chemical preservatives and facilitate the supply and demand of raw materials by mixing a natural extract for preservative consisting of a plurality of natural extracts with the cosmetic composition.
본 발명에 의한 화장료 조성물은 누에고치 유래 세리신(Sericin)과 알로에베라잎추출물(Aloe Barbadensis Leaf Extract)을 포함하되, 피부보습, 미백 및 주름개선효과를 나타내는 것을 특징으로 한다.The cosmetic composition according to the present invention includes cocoon-derived Sericin and Aloe Barbadensis Leaf Extract, and is characterized in that it exhibits skin moisturizing, whitening and wrinkle improvement effects.
또한, 본 발명에 의한 화장료 조성물의 상기 세리신은 황금누에고치로부터 추출된 것을 특징으로 한다.In addition, the sericin of the cosmetic composition according to the present invention is characterized in that it is extracted from the golden silkworm cocoon.
또한, 본 발명에 의한 화장료 조성물은, 피부진정 및 보습용 천연추출물을 더 포함하되, 상기 피부진정 및 보습용 천연추출물은 락토바실러스/콩발효추출물, 흰버드나무껍질추출물 및 쇠비름추출물로 이루어진 것을 특징으로 한다.In addition, the cosmetic composition according to the present invention further comprises a natural extract for soothing and moisturizing the skin, wherein the natural extract for soothing and moisturizing the skin is made of lactobacillus/fermented soybean extract, white willow bark extract and purslane extract. to be
또한, 본 발명에 의한 화장료 조성물은 방부용 천연추출물을 더 포함하되, 상기 방부용 천연추출물은, 육계나무껍질추출물, 오레가노잎추출물, 편백나무잎추출물 및 황금추출물로 이루어진 것을 특징으로 한다.In addition, the cosmetic composition according to the present invention further includes a natural extract for preservatives, and the natural extract for preservatives is characterized in that it consists of cinnamon bark extract, oregano leaf extract, cypress leaf extract and golden extract.
또한, 본 발명에 의한 화장료 조성물은 전체 중량에 대해, 상기 세리신은 0.01 내지 3 중량부, 상기 알로에베라잎추출물은 0.1 내지 3 중량부, 상기 보습 및 피부진정용 천연추출물 0.02 내지 3 중량부, 상기 방부용 천연추출물 0.02 내지 5 중량부를 각각 포함하는 것을 특징으로 한다.In addition, the cosmetic composition according to the present invention, based on the total weight, the sericin is 0.01 to 3 parts by weight, the aloe vera leaf extract is 0.1 to 3 parts by weight, the moisturizing and skin soothing natural extract is 0.02 to 3 parts by weight, the It is characterized by containing 0.02 to 5 parts by weight of natural extracts for preservatives, respectively.
본 발명은 누에고치 유래의 세리신과 알로에베라잎추출물을 혼합함으로써, 보습 및 피부진정, 미백, 주름개선 등의 효과가 있다.In the present invention, by mixing cocoon-derived sericin and aloe vera leaf extract, there are effects such as moisturizing and skin soothing, whitening, and wrinkle improvement.
또한 본 발명은 보습 및 피부진정용 천연추출물을 더 추가함으로써, 보습 및 피부진정 기능을 강화하고 원료의 수급을 유리하게 할 수 있다.In addition, the present invention can strengthen the moisturizing and skin soothing function and facilitate the supply and demand of raw materials by further adding a natural extract for moisturizing and skin soothing.
또한, 본 발명은 복수의 천연추출물로 이루어진 방부용 천연추출물을 혼합함으로써, 기존의 화학적 방부제를 대체할 수 있으며, 원료의 수급을 유리하게 할 수 있다.In addition, the present invention can replace existing chemical preservatives by mixing natural extracts for preservatives made of a plurality of natural extracts, and can advantageously supply and demand raw materials.
이하에서, 본 발명의 구체적인 실시예에 대해 상세하게 설명한다.Hereinafter, specific embodiments of the present invention will be described in detail.
본 발명에 의한 화장료 조성물은 누에고치 유래의 세리신(Sericin)과 알로에베라잎추출물(Aloe Barbadensis Leaf Extract)을 포함한다.The cosmetic composition according to the present invention includes Sericin derived from a silkworm cocoon and Aloe Barbadensis Leaf Extract.
상기 세리신은 누에고치 생사와 증류수의 욕비를 1:15~25로 하여 완전히 침지시킨 상태에서 80~120℃에서 1~3시간 동안 정련하여 누에고치 또는 황금누에고치로부터 세리신을 추출할 수 있다.The sericin can be extracted from silkworm cocoons or golden silkworm cocoons by refining at 80 to 120 ° C. for 1 to 3 hours in a completely immersed state with a water ratio of cocoon raw sand and distilled water of 1:15 to 25.
다만, 누에고치로부터 추출된 세리신은 고분자 물질로 물에 대한 용해도가 떨어지기 때문에, 본 발명에 있어서는, 추출된 세리신에 대해 알칼라아제 효소, 플라보자임 효소, 및 프로타멕스 효소를 각 1.5%(w/w)비율로 첨가하여 60℃에서 36h 동안 세리신을 가수분해시켰으며, 반응이 완전히 종료된 후, 효소를 불활성화시키기 위하여 1시간 동안 끊였다. However, since sericin extracted from silkworm cocoons is a high molecular substance and has low solubility in water, in the present invention, Alcalase enzyme, flavozyme enzyme, and protamex enzyme are each 1.5% with respect to the extracted sericin. Sericin was hydrolyzed at 60 ° C. for 36 h by adding at a (w / w) ratio, and after the reaction was completely completed, it was cut off for 1 hour to inactivate the enzyme.
또한, 가수분해를 거친 세리신에 남아 있는 불용성의 고분자 세리신를 제거하기 위하여, 상기 세리신을 증류수를 이용하여 2%용액으로 제조한 후, 2%용액을 원심분리(4000rpm/0.5hr)로 상등액을 분리한 후, Membrain filter(76mm디스크필터 10Kda, 1.0Kda /Millipore 사)을 사용하여, 여과 압력1.0~2.0 bar(N2 gas) 조건에서 교반하면서 분획 여과하였으며, 동결건조를 거쳐 분말화하였다.In addition, in order to remove the insoluble polymeric sericin remaining in the hydrolyzed sericin, the sericin was prepared as a 2% solution using distilled water, and then the 2% solution was centrifuged (4000 rpm / 0.5 hr) to separate the supernatant Then, using a membrane filter (76mm disk filter 10Kda, 1.0Kda /Millipore), fractionation was filtered while stirring under a filtration pressure of 1.0 to 2.0 bar (N 2 gas), and powdered through freeze-drying.
또한, 상기 세리신은 일반누에고치 또는 황금누에고치로부터 추출된 세리신이 바람직하게 이용될 수 있으며, 황금누에고치로부터 추출된 세리신이 더 바람직하다.In addition, sericin extracted from common silkworm cocoons or golden silkworm cocoons may be preferably used, and sericin extracted from golden silkworm cocoons is more preferred.
한편, 상기 알로에베라잎추출물은, 성체 알로에베라의 잎을 충분히 세척한 후, 건조하여 세절한 알로에베라잎 200g을 메탄올 2L에 넣고 3회에 걸쳐 실온에서 7일 동안 추출을 하였으며, 추출된 알로에베라잎추출물은 여과 후 진공회전 농축기로 45℃에서 감압 농축하여 추출 분말을 얻었다.On the other hand, for the aloe vera leaf extract, after sufficiently washing the adult aloe vera leaves, 200 g of dried and cut aloe vera leaves were put into 2 L of methanol and extracted three times at room temperature for 7 days, and the extracted aloe vera The leaf extract was filtered and then concentrated under reduced pressure at 45° C. using a vacuum rotary evaporator to obtain an extracted powder.
또한, 본 발명에 의한 화장료 조성물은 피부진정 및 보습용 천연추출물을 더 포함하되, 상기 피부진정 및 보습용 천연추출물은 락토바실러스/콩발효추출물, 흰버드나무껍질추출물 및 쇠비름추출물로 이루어진다.In addition, the cosmetic composition according to the present invention further includes a natural extract for soothing and moisturizing the skin, and the natural extract for soothing and moisturizing the skin consists of a lactobacillus/fermented soybean extract, a white willow bark extract, and a purslane extract.
상기 락토바실러스/콩발효추출물(Lactobacillus/Soybean Ferment Extract)은, 락토바실러스(Lactobacillus)로 콩을 발효하여 얻는 생성물을 추출한 것으로, 발효된 콩에서 생성되는 고분자 점액 물질이 우수한 보습력을 가져서 피부에 적용시에 피부 보습에 도움을 준다고 알려져 있다.The Lactobacillus/Soybean Ferment Extract is an extract of a product obtained by fermenting soybeans with Lactobacillus, and the polymeric mucus material produced from fermented soybeans has excellent moisturizing power when applied to the skin. It is known to help moisturize the skin.
상기 흰버드나무껍질추출물(Salix Alba(Willow) Bark Extract)은, 흰버드나무의 껍질에서 추출한 추출물로 탄닌과 살리실산(바하,BHA)가 함유되어 있어 피부의 각질 부드럽게 연화시켜 문제성 피부 진정를 진정시키며, 피부 속 수분 증발을 막아 피부의 보습력을 유지하는 효과가 있는 것으로 알려져 있다.The white willow bark extract (Salix Alba (Willow) Bark Extract) is an extract extracted from the bark of the white willow tree and contains tannin and salicylic acid (BHA), which gently softens the dead skin cells of the skin to soothe problem skin, It is known to have the effect of preventing the evaporation of moisture in the skin and maintaining the moisturizing power of the skin.
상기 쇠비름추출물(Portulaca Oleracea Extract)은 마치현 또는 오행초라고도 불리는 쇠비름의 추출물로서, 오메가 3, 탄닌, 사포닌, 베타카로틴 글로틴, 비타민류 등을 함유하고, 피부 진정과 자극 완화에 도움을 주는 것으로 알려져 있다. The purslane extract (Portulaca Oleracea Extract) is an extract of purslane, also called portulaca oleracea or five elements, and is known to contain omega 3, tannin, saponin, beta-carotene glotin, vitamins, etc., and to help soothe skin and relieve irritation. there is.
또한, 본 발명에 의한 화장료 조성물은 방부용 천연추출물을 더 포함하되, 상기 방부용 천연추출물은 육계나무껍질추출물, 오레가노잎추출물, 편백나무잎추출물 및 황금추출물로 이루어진다.In addition, the cosmetic composition according to the present invention further includes a natural extract for preservative use, and the natural extract for preservative is composed of cinnamon bark extract, oregano leaf extract, cypress leaf extract, and golden extract.
상기 육계나무껍질추출물(Cinnamomum Cassia Bark Extract)은 녹나무과에 속하는 육계나무의 건조한 수피를 추출한 추출물로서, 신남알데하이드(Cinnamic aldehyde), 캄펜(Camphene), 치네올(Cineol), 리날로올(Linalool), 유게놀(Eugenol) 등이 있으며, 항균 등의 효능이 있는 것으로 알려져 있다.The Cinnamomum Cassia Bark Extract is an extract extracted from the dried bark of a cinnamon tree belonging to the Lauraceae family, and contains cinnamic aldehyde, camphene, cineol, linalool, Eugenol and the like are known to have antibacterial and other effects.
상기 오레가노잎추출물(Origanum Vulgare Leaf Extract)은 오레가노의 입에서 추출한 추출물로서, 페놀 화합물, 특히 rosmarinic acid를 많이 함유하고 있으며, anti-inflammatory, antioxidant, antibacterial, antifungal 및 antiviral 특성을 지니고 있는 것으로 알려져 있다The oregano leaf extract (Origanum Vulgare Leaf Extract) is an extract extracted from the mouth of oregano, contains a lot of phenolic compounds, especially rosmarinic acid, and is known to have anti-inflammatory, antioxidant, antibacterial, antifungal and antiviral properties
상기 편백나무잎추출물(Chamaecyparis Obtusa Leaf Extract)은 노송나무, 히노끼라고도 불리는 편백나무의 잎에서 추출한 추출물로, 피톤치드라는 천연 항균물질을 함유하고 있는 것으로 알려져 있다.The cypress tree leaf extract (Chamaecyparis Obtusa Leaf Extract) is an extract extracted from the leaves of a cypress tree, also called cypress and hinoki, and is known to contain a natural antibacterial substance called phytoncide.
상기 황금추출물(Scutellaria Baicalensis Root Extract)은 꿀풀과 다년생 본초인 황금의 뿌리를 거피하여 건조한 것의 추출물로, 다양한 종류의 플라보노이드를 함유하고 항균 작용, 항염증 작용이 있는 것으로 알려져 있다.The golden extract (Scutellaria Baicalensis Root Extract) is an extract of peeled and dried roots of golden grass, a perennial herb, and contains various kinds of flavonoids and is known to have antibacterial and anti-inflammatory effects.
또한, 상기 화장료 조성물은, 상기 화장료 조성물의 전체 중량에 대해 상기 세리신 0.02 내지 3 중량%와, 상기 보습 및 피부진정용 천연추출물 0.02 내지 3 중량부%, 상기 방부용 천연추출물 0.2 내지 8 중량%를 포함한다.In addition, the cosmetic composition contains 0.02 to 3% by weight of the sericin, 0.02 to 3% by weight of the natural extract for moisturizing and skin soothing, and 0.2 to 8% by weight of the natural extract for preservatives, based on the total weight of the cosmetic composition. include
실시예 1: 세리신과 알로에베라잎추출물의 혼합 시료의 제조Example 1: Preparation of mixed samples of sericin and aloe vera leaf extract
<1-1> 세리신의 추출<1-1> Extraction of sericin
황금누에고치 생사와 증류수의 욕비를 1:15로 하여 완전히 침지시킨 상태에서 120℃에서 3시간 동안 정련하여 황금누에고치로부터 세리신을 추출한 후, 알칼라아제 효소, 플라보자임 효소, 및 프로타멕스 효소를 각 1.5%(w/w)비율로 첨가하여 60℃에서 36h 동안 세리신을 가수분해시켰으며, 반응이 완전히 종료된 후, 효소를 불활성화시키기 위하여 1시간 동안 끊였다. 또한, 가수분해를 거친 세리신 펩타이드 분말은 증류수를 이용하여 2%용액으로 제조하였으며, 2%용액을 원심분리(4000rpm/0.5hr)로 상등액을 분리한 후, Membrain filter(76mm디스크필터 10Kda, 1.0Kda /Millipore 사)을 사용하여, 여과 압력1.0~2.0 bar(N2 gas) 조건에서 교반하면서 분획 여과하였으며, 동결건조를 거쳐 분말화하였다.Sericin was extracted from the golden cocoon by refining at 120 ° C for 3 hours in a state where the golden silkworm cocoon was completely immersed in a water ratio of 1:15 to distilled water, and then, Alcalase enzyme, flavozyme enzyme, and Protamex Sericin was hydrolyzed at 60 ° C. for 36 h by adding enzymes at a rate of 1.5% (w / w), and after the reaction was completely finished, it was cut off for 1 hour to inactivate the enzyme. In addition, the hydrolyzed sericin peptide powder was prepared as a 2% solution using distilled water. /Millipore), fractionation was filtered while stirring at a filtration pressure of 1.0 to 2.0 bar (N 2 gas), and powdered through freeze-drying.
<1-2> 알로에베라잎추출물의 추출<1-2> Extraction of aloe vera leaf extract
성체 알로에베라의 잎을 충분히 세척한 후, 건조하여 세절한 알로에베라잎 200g을 메탄올 2L에 넣고 3회에 걸쳐 실온에서 7일 동안 추출을 하였다. 알로에베라잎추출물은 여과 후 진공회전 농축기로 45℃에서 감압 농축하여 추출 분말을 얻었다.After sufficiently washing the adult aloe vera leaves, 200 g of dried and cut aloe vera leaves were put into 2 L of methanol and extracted three times at room temperature for 7 days. After filtration, the aloe vera leaf extract was concentrated under reduced pressure at 45° C. using a vacuum rotary evaporator to obtain an extracted powder.
<1-3> 혼합시료의 제조<1-3> Preparation of mixed sample
상기 <1-1>과 <1-2>에 의하여 수득한 세리신 분말과 알로에베라잎추출물 분말을 동일 질량비로 혼합하여 시료(이하, "혼합시료"함)를 제조하였다.A sample (hereinafter referred to as "mixed sample") was prepared by mixing the sericin powder and the aloe vera leaf extract powder obtained by <1-1> and <1-2> in the same mass ratio.
시험예 1: 혼합시료의 항산화 효과Test Example 1: Antioxidant effect of mixed sample
혼합시료의 항산화 효과는 DPPH 라디칼 소거활성 및 ABTS 라디칼 소거활성을 측정하여 평가하였다. The antioxidant effect of the mixed sample was evaluated by measuring DPPH radical scavenging activity and ABTS radical scavenging activity.
DPPH는 항산화물질과 반응하여 음이온 라디칼이 소거되면서 보라색에서 노란색으로 탈색되는 원리로 항산화활성을 측정하는데 사용된다. 또한, ABTS 라디칼 소거활성도 DPPH을 이용한 방법과 함께 항산화활성을 측정하는데 많이 이용되는 방법인데, ABTS는 항산화물질과 반응하여 양이온 라디칼이 소거되면서 청록색에서 무색으로 탈색되는 원리로 항산화활성을 측정하는데 사용된다.DPPH is used to measure antioxidant activity by the principle of discoloration from purple to yellow as anion radicals are scavenged by reacting with antioxidants. In addition, ABTS radical scavenging activity is a method widely used to measure antioxidant activity along with the method using DPPH. ABTS reacts with antioxidants to scavenge cation radicals and is used to measure antioxidant activity on the principle of discoloration from bluish green to colorless. .
먼저, DPPH 라디칼 소거활성은 혼합시료에 대한 전자 공여능은 Bondet(1997)방법에 의해 측정하였다. 여러 농도의 혼합시료를 300μM DPPH/EtOH 760㎕에 첨가한 후 30분간 37℃에서 방치한 후 UV/Visible spectrophotometer(Berkman, USA)을 이용하여 515㎚에서 흡광도를 측정하였으며, 시료를 첨가하지 않은 대조군과의 차이에 의하여 활성을 측정하였다.First, the DPPH radical scavenging activity and the electron donating ability of the mixed sample were measured by the Bondet (1997) method. Mixed samples of various concentrations were added to 760 μl of 300 μM DPPH/EtOH, left at 37° C. for 30 minutes, and absorbance was measured at 515 nm using a UV/Visible spectrophotometer (Berkman, USA). Activity was measured by the difference between
또한, ABTS 라디칼 소거활성은 Roberty 등(1999)의 방법에 의해 측정하였다. ABTS 농도는 734㎚에서 1.500이 되도록 조제하였고, 농도를 달리한 혼합시료와 실온에서 10분간 반응 후 대조구와의 흡광도 차이에 의하여 활성을 측정하였다.In addition, ABTS radical scavenging activity was measured by the method of Roberty et al. (1999). The ABTS concentration was prepared to be 1.500 at 734 nm, and the activity was measured by the difference in absorbance with the control after reacting with the mixed sample with different concentrations at room temperature for 10 minutes.
또한, 혼합시료의 DPPH 라디칼 소거활성 및 ABTS 라디칼 소거활성에 대해, 대조구로서 아스코르빈산(L-ascorbicacid)의 활성과 비교한 결과, 아래의 [표 1]과 같이 나타났다.In addition, as a result of comparing the DPPH radical scavenging activity and ABTS radical scavenging activity of the mixed sample with the activity of ascorbic acid (L-ascorbicacid) as a control, the following [Table 1] was shown.
| 활성별by activity | 혼합시료의 농도(㎍/㎖)Concentration of mixed sample (μg/ml) | |||||
| 22 | 44 | 1010 | 4040 | 200200 | 대조구*control* | |
| DPPH(%)DPPH (%) | 36.236.2 | 48.748.7 | 60.160.1 | 87.287.2 | 97.397.3 | 98.898.8 |
| ABTS(%)ABTS (%) | 38.438.4 | 50.250.2 | 67.267.2 | 94.394.3 | 99.199.1 | 99.699.6 |
* 대조구: 아스코르빈산(L-ascorbicacid)의 200㎍/㎖일 때의 활성* Control: activity at 200 μg/ml of ascorbic acid (L-ascorbicacid)
위의 [표 1]에서 보는 바와 같이, 세리신과 알로에베라잎추출물을 동일 질량비로 혼합한 경우, DPPH 라디칼 소거활성 및 ABTS 라디칼 소거활성능은 농도 의존적으로 증가하는 것으로 나타났으며, 또한 동일농도(200㎍/㎖)에서 대조구인 아스코르빈산(L-ascorbicacid)에 대해 DPPH 라디칼 소거활성은 98.5%, ABTS 라디칼 소거활성은 99.5%의 활성효과가 나타났다. 즉 세리신과 알로에베라잎추출물을 동일 질량비로 혼합한 경우 항산화능이 우수한 소재로 활용할 수 있다. As shown in [Table 1] above, when sericin and aloe vera leaf extract were mixed at the same mass ratio, the DPPH radical scavenging activity and the ABTS radical scavenging activity were found to increase in a concentration-dependent manner, and also at the same concentration ( 200 μg/ml), the DPPH radical scavenging activity was 98.5% and the ABTS radical scavenging activity was 99.5% for the control ascorbic acid (L-ascorbicacid). That is, when sericin and aloe vera leaf extract are mixed in the same mass ratio, it can be used as a material with excellent antioxidant activity.
시험예 2: 혼합시료의 미백 효과Test Example 2: Whitening effect of mixed sample
혼합시료의 미백효능은 멜라닌의 생성을 조절하는 산화 효소인 tyrosinase의 저해활성으로 평가하였다. The whitening effect of the mixed samples was evaluated by the inhibitory activity of tyrosinase, an oxidizing enzyme that regulates the production of melanin.
Tyrosinase 저해활성 측정은 Yagi 등의 방법에 따라 측정하였다. 반응구는 1/15M sodium phosphate buffer (pH 6.8) 0.5mL에 10mM L-DOPA을 녹인 기질액 0.2mL 및 시료용액 0.1mL의 혼합액에 mushroom tyrosinase (110U/mL) 0.2mL 첨가하여 25℃에서 2분간 반응시켜 반응액 중에 생성된 DOPA chrome을 475㎚에서 측정하였으며, Tyrosinase 저해활성은 시료용액의 첨가구와 무첨가구의 흡광도 감소율로 나타낸다.Tyrosinase inhibitory activity was measured according to the method of Yagi et al. In the reaction zone, 0.2mL of mushroom tyrosinase (110U/mL) was added to a mixture of 0.2mL of substrate solution in which 10mM L-DOPA was dissolved in 0.5mL of 1/15M sodium phosphate buffer (pH 6.8) and 0.1mL of sample solution, followed by reaction at 25℃ for 2 minutes. The DOPA chrome generated in the reaction solution was measured at 475 nm, and the Tyrosinase inhibitory activity is expressed as the absorbance decrease rate of the sample solution added and unadded.
여러 농도의 혼합시료에 대해 측정한 Tyrosinase 저해활성을 대조구로서 알부틴의 활성과 비교한 결과, 아래의 [표 2]와 같이 나타났다.As a result of comparing the Tyrosinase inhibitory activity measured for mixed samples of various concentrations with the activity of arbutin as a control, it was shown in [Table 2] below.
| 활성별by activity | 혼합시료의 농도(㎍/㎖)Concentration of mixed sample (μg/ml) | |||||
| 22 | 44 | 1010 | 4040 | 200200 | 대조구*control* | |
| Tyrosinase(%)Tyrosinase (%) | 11.311.3 | 19.419.4 | 26.826.8 | 40.240.2 | 60.760.7 | 68.568.5 |
* 대조구: 알부틴의 200㎍/㎖일 때의 활성* Control: activity at 200 μg/ml of arbutin
위의 [표 2]에서 보는 바와 같이, 세리신과 알로에베라잎추출물을 동일 질량비로 혼합한 혼합시료의 경우 멜라닌의 생성을 조절하는 산화 효소인 Tyrosinase의 저해활성이 농도 의존적으로 증가하는 것으로 나타났으며, 또한 동일농도(200㎍/㎖)에서 대조구인 알부틴에 대해 88.6%의 Tyrosinase 저해활성의 효과가 있는 것으로 나타났다.As shown in [Table 2] above, in the case of a mixed sample in which sericin and aloe vera leaf extract were mixed at the same mass ratio, the inhibitory activity of Tyrosinase, an oxidizing enzyme that controls melanin production, was found to increase in a concentration-dependent manner. , Also, at the same concentration (200 μg/ml), it was found to have an 88.6% Tyrosinase inhibitory activity against arbutin, a control group.
시험예 3: 혼합시료의 주름개선 효과Test Example 3: Wrinkle improvement effect of mixed sample
피부주름 개선에는 콜라겐(collagen)뿐만 아니라 탄력섬유인 엘라스틴(elastin) 등도 기여하는 것으로 보고되고 있는데, 특히 자외선 조사 후 엘라스틴을 분해하는 엘라스타제(elastase)의 활성이 증가하기 때문에 엘라스타제의 활성증가는 자외선에 의한 피부 탄성도의 감소 및 주름 생성의 주요원인으로 생각되고 있다. 따라서, 혼합시료의 주름개선 효능은 Elastase 저해활성으로 평가하였다. It is reported that not only collagen but also elastin, an elastic fiber, contributes to the improvement of skin wrinkles. The increase is considered to be the main cause of the reduction of skin elasticity and wrinkles caused by ultraviolet rays. Therefore, the anti-wrinkle efficacy of the mixed sample was evaluated by Elastase inhibitory activity.
Elastase 저해활성은 여러 농도의 혼합시료 0.1㎖에 elastase(0.6 units/㎖) in 50mM Tris-HCl buffer(pH 8.6) 용액 0.05㎖를 가한 후 N-succinyl-(L-Ala)3-p-nitroanilide(1mg/㎖) in 50mM Tris-HCl buffer (pH 8.6) 0.1㎖를 첨가하여 30분간 반응 후 410㎚에서 흡광도를 측정하였으며, 대조구로서 레티놀산의 활성과 비교한 결과, 아래의 [표 3]과 같이 나타났다.Elastase inhibitory activity was measured by adding 0.05 ml of elastase (0.6 units/ml) in 50 mM Tris-HCl buffer (pH 8.6) solution to 0.1 ml of a mixed sample of various concentrations, and then measuring N-succinyl-(L-Ala)3-p-nitroanilide ( 1mg/ml) in 50mM Tris-HCl buffer (pH 8.6) 0.1ml was added, reacted for 30 minutes, and absorbance was measured at 410nm. As a result of comparison with the activity of retinoic acid as a control, as shown in [Table 3] below appear.
| 활성별by activity | 혼합시료의 농도(㎍/㎖)Concentration of mixed sample (μg/ml) | |||||
| 22 | 44 | 1010 | 4040 | 200200 | 대조구*control* | |
| Elastase(%)Elastase (%) | 5.85.8 | 14.414.4 | 27.627.6 | 41.741.7 | 65.465.4 | 73.573.5 |
* 대조구: 레티놀산의 200㎍/㎖일 때의 활성* Control: activity at 200 μg/ml of retinoic acid
위의 [표 3]에서 보는 바와 같이, 홉합시료의 Elastase 저해활성은 농도 의존적으로 증가하는 것으로 나타났으며, 또한 동일농도(200㎍/㎖)에서 대조구인 레티놀산에 대해 88.9%의 Elastase 저해활성의 효과가 있는 것으로 나타났다.As shown in [Table 3] above, the Elastase inhibitory activity of the hop sample was found to increase in a concentration-dependent manner, and at the same concentration (200μg/ml), 88.9% of the Elastase inhibitory activity against the control retinoic acid has been shown to be effective.
실시예 2: 피부진정 및 보습용 천연추출물의 제조Example 2: Preparation of natural extracts for skin soothing and moisturizing
<2-1> 락토바실러스/콩발효추출물의 제조<2-1> Preparation of Lactobacillus/Soybean Fermented Extract
대두콩을 세척 및 건조하여 분말화 한 콩 분말에 정제수를 1:7의 중량비로 가하고 90℃에서 10시간 동안 환류추출한 후, 여과포로 1차 여과하고, 다시 0.4~0.5㎛수준의 여과필터로 2차 여과하여 콩추출액을 얻었으며, 상기 콩추출액은 멸균 후 동결건조를 거쳐 분말화하였다.After washing and drying soybeans, purified water was added in a weight ratio of 1:7 to powdered soybean powder, and reflux extraction was performed at 90 ° C for 10 hours. The soybean extract was obtained by secondary filtration, and the soybean extract was sterilized and lyophilized to powder.
증류수 100㎖에 건조시킨 콩추출물 분말을 3%(w/v), 글루코스 0.4%(v/v) 및 효모추출물 0.2%(w/v)를 첨가하여 멸균시킨 배지를 제조한 후, 활성화된 L. plantarum과 L. brevis를 각각 2%(v/v)씩을 동시에 접종하는 동시 배양법을 적용하였으며, 배양액을 37oC, 120 rpm에서 배양하면서 24시간 발효시켜 락토바실러스/콩발효추출물을 제조하였다.After preparing a sterilized medium by adding 3% (w / v) of dried soybean extract powder, 0.4% (v / v) of glucose and 0.2% (w / v) of yeast extract to 100 ml of distilled water, activated L A simultaneous culture method of inoculating 2% (v/v) of plantarum and L. brevis at the same time was applied, and the culture medium was fermented for 24 hours while culturing at 37 ° C and 120 rpm to prepare a Lactobacillus / soybean fermentation extract.
발효된 락토바실러스/콩발효추출물은 원심 분리 및 0.4~0.5㎛ 수준의 여과필터로 1차 여과하고, 배양균 및 찌꺼기를 제거한 후, 0.3㎛이하의 여과막을 이용하여 2차 여과하였으며, 동결건조를 거쳐 분말화하였다.The fermented Lactobacillus/soybean fermented extract was firstly filtered by centrifugal separation and a filter of 0.4 to 0.5㎛ level, and after removing cultured bacteria and debris, it was secondarily filtered using a filtration membrane of 0.3㎛ or less, and freeze-dried. It was then powdered.
<2-2> 흰버드나무껍질추출물 및 쇠비름추출물의 제조<2-2> Preparation of white willow bark extract and purslane extract
흰버드나무껍질 및 쇠비름 전초를 충분히 세척후 건조하여 분쇄한 후, 흰버드나무껍질 및 쇠비름 전초 각각 200g을 메탄올 2L에 넣고 3회에 걸쳐 실온에서 7일 동안 추출을 하였다. 각 추출물은 여과 후 진공회전 농축기로 45℃에서 감압 농축하여 각각의 추출 분말을 수득하였다.After thoroughly washing the white willow bark and purslane sheath, drying and pulverizing, 200 g of each of the white willow bark and purslane sheath were put into 2 L of methanol and extracted three times at room temperature for 7 days. Each extract was filtered and then concentrated under reduced pressure at 45° C. using a vacuum rotary evaporator to obtain each extracted powder.
<2-3> 피부진정 및 보습용 천연추출물의 시료의 제조<2-3> Preparation of samples of natural extracts for skin soothing and moisturizing
상기 <2-1> 및 <2-2>에 의해 수득한 상기 락토바실러스/콩발효추출물, 흰버드나무껍질추출물 및 쇠비름추출물 각각을 동일 질량비로 혼합하여 시료를 제조하였다.Samples were prepared by mixing each of the lactobacillus/soybean fermented extract, white willow bark extract, and purslane extract obtained by <2-1> and <2-2> in the same mass ratio.
시험예 4: 피부진정 및 보습용 천연추출물의 혼합시료에 대한 활성저해 여부Test Example 4: Activity inhibition of mixed samples of natural extracts for skin soothing and moisturizing
세리신과 알로에베라잎추출물을 동일 질량비로 혼합한 혼합시료에 피부진정 및 보습용 천연추출물 시료를 더 포함하는 경우, 더 포함된 피부진정 및 보습용 천연추출물 시료에 의해서 혼합시료의 DPPH 라디컬 소거활성, ABTS 라디칼 소거활성 및 tyrosinase억제활성이 저해되는지 여부를 검토하였다.When a sample of natural extract for skin soothing and moisturizing is further included in a mixed sample of sericin and aloe vera leaf extract mixed in the same mass ratio, DPPH radical scavenging activity of the mixed sample by the further included sample of natural extract for soothing and moisturizing skin , it was examined whether ABTS radical scavenging activity and tyrosinase inhibitory activity were inhibited.
이때, 상기 혼합시료의 양(질량)을 일정하게 한 상태에서, 상기 혼합시료의 양 대비 상기 피부진정 및 보습용 천연추출물 시료의 추가비율(질량비)을 달리하여 상기 피부진정 및 보습용 천연추출물 시료를 혼합하였으며, 상기 혼합시료와 피부진정과 보습용 천연추출물 시료가 혼합된 시료의 농도를 200㎍/㎖로 하여, 상기 피부진정 및 보습용 천연추출물의 추가비율에 따른 DPPH 라디컬 소거활성, ABTS 라디칼 소거활성, tyrosinase 억제활성 및 Elastase 억제활성에 대해 측정하였다,At this time, in a state where the amount (mass) of the mixed sample is kept constant, the additional ratio (mass ratio) of the natural extract sample for skin soothing and moisturizing is different from the amount of the mixed sample, so that the natural extract sample for soothing and moisturizing the skin was mixed, and the concentration of the sample mixed with the mixed sample and the natural extract sample for skin soothing and moisturizing was 200 μg / ml, DPPH radical scavenging activity according to the additional ratio of the natural extract for skin soothing and moisturizing, ABTS Radical scavenging activity, tyrosinase inhibitory activity, and elastase inhibitory activity were measured.
|
혼합비율 (A:B)*mixing ratio (A:B)* |
DPPH radical 소거활성(%)DPPH radical Clearing activity (%) |
ABTS radical 소거활성(%)ABTS radical Clearing activity (%) |
Tyrosinase 억제활성(%)Tyrosinase Inhibitory activity (%) |
Elastase 억제활성(%)Elastase Inhibitory activity (%) |
| 1:01:0 | 97.397.3 | 99.199.1 | 60.760.7 | 65.465.4 |
| 30:130:1 | 97.397.3 | 99.199.1 | 60.960.9 | 65.465.4 |
| 20:120:1 | 97.497.4 | 99.199.1 | 61.161.1 | 65.565.5 |
| 10:110:1 | 97.697.6 | 99.399.3 | 61.261.2 | 65.865.8 |
| 5:15:1 | 97.697.6 | 99.299.2 | 61.461.4 | 65.965.9 |
| 3:13:1 | 97.897.8 | 99.399.3 | 61.461.4 | 66.366.3 |
| 1:11:1 | 98.098.0 | 99.399.3 | 61.761.7 | 66.566.5 |
*: A(혼합시료)와 B(피부진정 및 보습용 천연추출물 시료)의 혼합비*: Mixing ratio of A (mixed sample) and B (natural extract sample for skin soothing and moisturizing)
위의 [표 4]에서 볼 수 있는 바와 같이, 락토바실러스/콩발효추출물, 흰버드나무껍질추출물 및 쇠비름추출물이 동일 질량비로 혼합된 피부진정 및 보습용 천연추출물 시료를 상기 세리신과 알로에베라잎추출물을 동일 질량비로 혼합한 혼합시료에 더 추가하더라도 상기 혼합시료의 DPPH 라디컬 소거활성, ABTS 라디칼 소거활성, tyrosinase 억제활성 및 Elastase 억제활성이 저해되지 않는 것으로 나타났다.As can be seen in [Table 4] above, the sericin and aloe vera leaf extract samples of natural extracts for skin soothing and moisturizing, in which lactobacillus/soybean fermented extract, white willow bark extract, and purslane extract were mixed in the same mass ratio, It was found that DPPH radical scavenging activity, ABTS radical scavenging activity, tyrosinase inhibitory activity, and Elastase inhibitory activity of the mixed sample were not inhibited even when further added to the mixed sample mixed in the same mass ratio.
시험예 5: 피부진정 및 보습용 천연추출물의 피부보습 효과Test Example 5: Skin moisturizing effect of natural extracts for skin soothing and moisturizing
상기 피부진정 및 보습용 천연추출물의 피부보습 효과는, 피부가 건조하다고 느끼고 있는 성인 여성 10명(이하, "시험 대상자"라 함)을 대상으로 상기 피부진정 및 보습용 천연추출물 시료(농도:200㎍/㎖)를 도포한 후 시간 경과에 따른 피부 수분도 변화를 Corneometer(CM 825)로 측정하여 평가하였다.The skin moisturizing effect of the natural extract for soothing and moisturizing the skin was measured in 10 adult women (hereinafter referred to as "test subjects") who felt their skin was dry, and the natural extract for soothing and moisturizing the skin (concentration: 200 After application of ㎍/㎖), the change in skin moisture level over time was measured and evaluated with a Corneometer (CM 825).
구체적으로 시험 대상자들의 손을 세정 및 건조한 후, 손등을 도포부위와 비도포부위로 나누고, 도포부위에 상기 피부진정 및 보습용 천연추출물 시료를 도포하기 직전, 도포직후, 도포후 30분 경과후, 60분 경과후, 90분 경과후, 120분 경과후에 도포부위의 피부 수분도를 측정하였으며, 도포부위의 피부 수분도를 측정과 동시에 비도포부위의 피부수분도도 측정하였다. 아래의 [표 5]는 시험 대상자 10명의 평균 값이며, 단위는 제조사의 A.U.(arbitary unit)이다.Specifically, after washing and drying the hands of the test subjects, the back of the hand was divided into an applied area and a non-application area, and immediately before, immediately after application, and after 30 minutes after application of the natural extract sample for skin soothing and moisturizing on the application area, 60 After lapse of minutes, after 90 minutes, and after 120 minutes, the skin moisture level at the application site was measured, and at the same time, the skin moisture level at the application site was measured and the skin moisture level at the non-application site was also measured. [Table 5] below is the average value of 10 test subjects, and the unit is A.U. (arbitary unit) of the manufacturer.
|
도포 직전apply eve |
도포 후 경과된 시간(분)Elapsed time (minutes) after application | |||||
| 00 | 3030 | 6060 | 9090 | 120120 | ||
| 도포부위application area | 31.431.4 | 58.758.7 | 50.250.2 | 46.346.3 | 42.442.4 | 37.537.5 |
| 비도포부위non-applied area | 31.331.3 | 31.331.3 | 29.329.3 | 28.528.5 | 27.927.9 | 27.927.9 |
제조사의 기준에 의하면, 30 A.U.이하: Very dry skin, 30-40 A.U.: Dry skin, 40 A.U.이상: Normal skin으로 정하고 있다. 제조사의 기준에 따라 위의 [표 5]를 살펴보면, 시험 대상자들의 평균 피부(손등) 상태는 매우 건조한 상태(Very dry skin)임을 알 수 있고, 상기 피부진정 및 보습용 천연추출물 시료(200㎍/㎖)를 도포함으로써 도포 후 90분 정도까지는 보통상태(Normal skin)을 유지할 수 있는 것으로 나타났다.According to the manufacturer's standards, less than 30 A.U.: Very dry skin, 30-40 A.U.: Dry skin, and more than 40 A.U.: Normal skin. Looking at [Table 5] above according to the manufacturer's standards, it can be seen that the average skin (back of the hand) condition of the test subjects was very dry skin, and the natural extract sample for skin soothing and moisturizing (200 μg / ㎖), it was found that normal skin could be maintained up to about 90 minutes after application.
실시예 3: 방부용 천연추출물의 제조Example 3: Preparation of natural extracts for preservatives
<3-1> 육계나무껍질, 오레가노잎, 편백나무잎, 황금의 추출물 제조<3-1> Manufacture of cinnamon bark, oregano leaves, cypress leaves, and golden extracts
육계나무의 껍질, 오레가노의 잎, 편백나무의 잎, 황금(뿌리) 각각을 충분히 세척후 건조하여 분쇄한 후, 분쇄된 육계나무껍질, 오레가노잎, 편백나무잎, 황금 각각 200g을 메탄올 2L에 넣고 3회에 걸쳐 실온에서 7일 동안 추출을 하였다. 각 추출물은 여과 후 진공회전 농축기로 45℃에서 감압 농축하여 각각의 추출 분말을 수득하였다.Thoroughly wash each of the cinnamon bark, oregano leaf, cypress leaf, and gold (root), dry and pulverize, and then add 200g of each of the crushed cinnamon bark, oregano leaf, cypress leaf, and gold to 2L of methanol. Extraction was performed three times at room temperature for 7 days. Each extract was filtered and then concentrated under reduced pressure at 45° C. using a vacuum rotary evaporator to obtain each extracted powder.
<3-2> 방부용 천연추출물 시료의 제조<3-2> Preparation of natural extract samples for preservation
상기 육계나무껍질추출물, 오레가노잎추출물, 편백나무잎추출물 및 황금추출물을 동일 질량비로 혼합하여 방부용 천연추출물 시료를 제조하였다.Natural extract samples for preservatives were prepared by mixing the cinnamon bark extract, oregano leaf extract, cypress leaf extract, and golden extract in the same mass ratio.
시험예 6: 각 천연추출물과 방부용 천연추출물의 방부효과Test Example 6: Preservative effect of each natural extract and natural extract for preservative
육계나무껍질추출물 시료(시료 1), 오레가노잎추출물 시료(시료 2), 편백나무잎추출물 시료(시료 3). 황금추출물 시료(시료 4) 및 방부용 천연추출물 시료(시료 5) 각각의 세균에 대한 방부력를 평가하였다.Cassia bark extract sample (Sample 1), oregano leaf extract sample (Sample 2), cypress leaf extract sample (Sample 3). The gold extract sample (Sample 4) and the preservative natural extract sample (Sample 5) were evaluated for their preservative power against bacteria.
각 시료(시료 1~5)의 방부력 평가는, 각 시료에 세균으로 스타필로코커스 아우레우스(Staphylococcus aureus), 에스케리치아 콜리(Escherichia coli), 및 슈도모나스 아에루기노사(Paeudomonas aeruginosa) 3가지의 혼합균액을 각 시료 1g 당 1.6X106개가 되도록 접종을 하고, 접종한 날부터 1일, 3일, 5일, 7일, 14일, 21일, 28일 간격으로 각 시료를 1g씩 취하여 생균 수를 측정하였으며, 그 결과는 아래의 [표 5]와 같다. In the evaluation of the preservative power of each sample (Samples 1 to 5), Staphylococcus aureus, Escherichia coli, and Paeudomonas aeruginosa were 3 bacteria in each sample. Inoculate the mixed bacterial solution of eggplant so that 1.6X10 6 per 1g of each sample is inoculated, and take 1g of each sample at intervals of 1st, 3rd, 5th, 7th, 14th, 21st and 28th days from the date of inoculation The number of viable cells was measured, and the results are shown in [Table 5] below.
방부력 유효성으로, 세균은 접종 7일 이내에 99.9% 이상 균수가 감소해야 하며, 시험기간 동안 증식이 없어야 한다. For antiseptic efficacy, the number of bacteria should be reduced by 99.9% or more within 7 days of inoculation, and there should be no proliferation during the test period.
| 시료의 종류Sample type |
접종균수 (개/g)inoculum count (pcs/g) |
기간의 경과에 따른 생균 수(개/g)Viable cell count over time (unit/g) | ||||||
| 1일1 day | 3일3 days | 5일5 days | 7일7 days | 14일14 days | 21일21st | 28일28 days | ||
| 시료 1Sample 1 | 1.6X106 1.6X10 6 | 5.6X104 5.6X10 4 | 4.7X103 4.7X10 3 | <10<10 | <10<10 | <10<10 | <10<10 | <10<10 |
| 시료 2sample 2 | 1.6X106 1.6X10 6 | 5.3X104 5.3X10 4 | 3.6X103 3.6X10 3 | <10<10 | <10<10 | <10<10 | <10<10 | <10<10 |
| 시료 3sample 3 | 1.6X106 1.6X10 6 | 3.6X104 3.6X10 4 | 2.8X103 2.8X10 3 | <10<10 | <10<10 | <10<10 | <10<10 | <10<10 |
| 시료 4sample 4 | 1.6X106 1.6X10 6 | 4.3X104 4.3X10 4 | 2.1X103 2.1X10 3 | <10<10 | <10<10 | <10<10 | <10<10 | <10<10 |
| 시료 5sample 5 | 1.6X106 1.6X10 6 | 3.1X104 3.1X10 4 | 1.9X103 1.9X10 3 | <10<10 | <10<10 | <10<10 | <10<10 | <10<10 |
위의 [표 6]에서 보는 바와 같이, 본 발명의 방부용 천연추출물에 사용되는 각 천연추출물 및 각 천연추출물을 혼합한 방부용 천연추출물의 경우, 모두 세균 접종일로부터 5일이내에 생균수가 99.9% 이상 감소하였고, 향후 증식이 일어나지 않았다는 점에서, 모두 유효한 방부효과를 나타내고 있음을 확인하였다. 즉, 본 발명의 방부용 천연추출물 내에서 원료의 수급에 따라 각 천연추출물들을 적절하게 조절하여 사용할 수 있음을 확인하였다.As shown in [Table 6] above, in the case of each natural extract used in the natural extract for preservatives of the present invention and the natural extract for preservatives in which each natural extract was mixed, the viable cell count was 99.9% within 5 days from the date of bacterial inoculation. It was confirmed that all of them exhibited an effective preservative effect in that they were reduced and no future proliferation occurred. That is, it was confirmed that each natural extract can be appropriately adjusted and used according to the supply and demand of raw materials within the natural extract for preservatives of the present invention.
본 발명은 누에고치 유래 세리신과 알로에베라잎추출물을 포함하는 화장료 조성물로서 피부보습 및 진정, 미백, 주름개선 등에 효과가 있는 것으로 산업상 이용가능성이 있다.The present invention is a cosmetic composition containing cocoon-derived sericin and aloe vera leaf extract, which is effective for skin moisturizing and soothing, whitening, and wrinkle improvement, and has industrial applicability.
Claims (5)
- 누에고치 유래 세리신(Sericin)과 알로에베라잎추출물(Aloe Barbadensis Leaf Extract)을 포함하되, 피부보습, 미백 및 주름개선효과를 나타내는 것을 특징으로 하는 화장료 조성물.A cosmetic composition comprising cocoon-derived Sericin and Aloe Barbadensis Leaf Extract, characterized in that it exhibits skin moisturizing, whitening and wrinkle-reducing effects.
- 제1항에 있어서,According to claim 1,상기 세리신은 황금누에고치로부터 추출된 것을 특징으로 하는 화장료 조성물.The sericin is a cosmetic composition, characterized in that extracted from the golden silkworm cocoon.
- 제1항 또는 제2항에 있어서,According to claim 1 or 2,피부진정 및 보습용 천연추출물을 더 포함하되, 상기 피부진정 및 보습용 천연추출물은 락토바실러스/콩발효추출물, 흰버드나무껍질추출물 및 쇠비름추출물로 이루어진 것을 특징으로 하는 화장료 조성물.A cosmetic composition further comprising a natural extract for soothing and moisturizing the skin, wherein the natural extract for soothing and moisturizing the skin is composed of a lactobacillus/soybean fermented extract, a white willow bark extract, and a purslane extract.
- 제3항에 있어서,According to claim 3,방부용 천연추출물을 더 포함하되, Further comprising a natural extract for preservatives,상기 방부용 천연추출물은, 육계나무껍질추출물, 오레가노잎추출물, 편백나무잎추출물 및 황금추출물로 이루어진 것을 특징으로 하는 화장료 조성물.The natural extract for preservatives is a cosmetic composition, characterized in that consisting of cinnamon bark extract, oregano leaf extract, cypress leaf extract and golden extract.
- 제4항에 있어서,According to claim 4,전체 중량에 대해, 상기 세리신은 0.01 내지 3 중량%, 상기 알로에베라잎추출물은 0.1 내지 3 중량%, 상기 피부진정 및 보습용 천연추출물 0.02 내지 3 중량%, 상기 방부용 천연추출물 0.02 내지 5 중량%를 각각 포함하는 것을 특징으로 하는 화장료 조성물.With respect to the total weight, the sericin is 0.01 to 3% by weight, the aloe vera leaf extract is 0.1 to 3% by weight, the skin soothing and moisturizing natural extract is 0.02 to 3% by weight, the preservative natural extract is 0.02 to 5% by weight A cosmetic composition characterized in that each comprises a.
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| KR10-2021-0090152 | 2021-07-09 | ||
| KR1020210090152A KR102629375B1 (en) | 2021-07-09 | 2021-07-09 | Cosmetic Composition Containing Sericin Derive From Silkworm and Aloe Barbadensis Leaf Extract |
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| KR20170065959A (en) | 2015-12-04 | 2017-06-14 | (주)화니핀코리아 | Cosmetic Composition For Mask Pack Comprising Extract Of Mangosteen And Sericin Extracted From Golden Silk Silkworm Cocoon |
| KR102035590B1 (en) | 2017-06-26 | 2019-10-23 | (주)두영티앤에스 | Cosmetic composition for scalp protection and hair improvement containing ginsenoside F5 derive from aerial part of hydroponic ginseng and gold sericin derive from golden silkworm |
| KR102409404B1 (en) | 2019-09-18 | 2022-06-15 | 주식회사 엘지생활건강 | Cosmetic Composition Comprising High Molecular Weight Sericin |
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| KR20050015177A (en) * | 2003-08-04 | 2005-02-21 | 한국식품연구원 | Face-pack Composition Comprising Sericin Protein Extracted from Cocoon |
| KR20130108805A (en) * | 2012-03-26 | 2013-10-07 | (주)더페이스샵 | Cosmetic composition comprising golden silkworm(bombyx moril) derived materials as active ingredient |
| KR20160139324A (en) * | 2015-05-27 | 2016-12-07 | 여명바이오켐 주식회사 | Cosmetic composition for skin whitening with peptide from golden silkworm |
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