KR20050015177A - Face-pack Composition Comprising Sericin Protein Extracted from Cocoon - Google Patents

Face-pack Composition Comprising Sericin Protein Extracted from Cocoon

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Publication number
KR20050015177A
KR20050015177A KR1020030053873A KR20030053873A KR20050015177A KR 20050015177 A KR20050015177 A KR 20050015177A KR 1020030053873 A KR1020030053873 A KR 1020030053873A KR 20030053873 A KR20030053873 A KR 20030053873A KR 20050015177 A KR20050015177 A KR 20050015177A
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South Korea
Prior art keywords
powder
sericin
cocoon
sericin protein
pack
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KR1020030053873A
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Korean (ko)
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KR100542266B1 (en
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임성일
김성수
이장은
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한국식품연구원
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
    • A61K8/987—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
    • A61K8/0212—Face masks
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/74—Biological properties of particular ingredients
    • A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
    • A61K2800/782—Enzyme inhibitors; Enzyme antagonists
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/84—Products or compounds obtained by lyophilisation, freeze-drying

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Birds (AREA)
  • Epidemiology (AREA)
  • Zoology (AREA)
  • Dermatology (AREA)
  • Cosmetics (AREA)

Abstract

PURPOSE: Provided is a facacil pack composition comprising, as an active ingredient, sericin protein extracted from the cocoon. The sericin protein has hihg water absorbability and viscosity, and inhibits the activity of tyrosinase. Therefore, the composition added with the sericin protein has excellent skin whitening effect. CONSTITUTION: The facial pack composition is characterized by containing sericin protein extracted from the cocoon as an active ingredient, wherein the extract is added in the form of freeze-dried powder. It further contains at least one selected from the group consisting of grape seed powder, apricot seed powder, job's tear powder, potato flour, green tea powder, chestnut skin powder, pine mushroom powder, lemon juice, vitamin C and le mon flavor.

Description

누에고치로부터 추출된 세리신 단백질을 함유하는 미용팩 조성물{Face-pack Composition Comprising Sericin Protein Extracted from Cocoon}Face pack composition containing sericin protein extracted from cocoon {Face-pack Composition Comprising Sericin Protein Extracted from Cocoon}

본 발명은 누에고치로부터 추출된 세리신 단백질을 함유하는 미용팩에 관한 것으로서, 보다 상세하게는 누에고치로부터 열수 추출한 세리신 단백질이 수분흡수력 및 점성을 가지며 티로시나아제 저해활성을 가지는 특성을 이용하여 이를 기능성이 가미된 미용팩의 활성성분으로 하는 조성물에 관한 것이다.The present invention relates to a cosmetic pack containing sericin protein extracted from silkworm cocoon, and more specifically, sericin protein hydrothermally extracted from silkworm cocoon using water-absorbing and viscous properties and having tyrosinase inhibitory activity. It relates to a composition comprising the active ingredient of the cosmetic packs added.

세리신은 견교(絹膠)·실크글루(silk glue)라고도 하며, 누에고치 섬유는 2가닥의 피브로인이 3층의 세리신으로 덮여 있다. 1865년 E.크래머가 누에고치 섬유에서 분리하여 비단을 뜻하는 라틴어 sercum 및 그리스어 serikon을 따서 이를 세리신으로 명명하였다. 생사(生絲)의 거칠고 딱딱한 느낌은 세리신이 부착되어 있기 때문인데, 정련하면 세리신이 녹아 없어져서 견사(絹絲) 특유의 감촉이 된다. 세리신은 아미노산 조성(組成) 중 세린이 37몰％ 정도로서 뚜렷하게 많으며, 뜨거운 물에 녹고 식으면 겔화하는 특징이 있다. 또한, 본 발명자의 연구에 의하면 티로시나아제 저해활성과 수분흡수성 및 점도가 매우 우수한 것으로 밝혀졌다. Sericin is also called silk glue and silkworm cocoon fibers are covered with two layers of fibroin with three layers of sericin. In 1865, E. Kramer separated it from the cocoon fibers and named it sericin after the Latin sercum, which means silk, and the Greek serikon. The rough and hard feeling of raw silk is due to the attachment of sericin. When refined, sericin melts away and becomes a silky texture. Sericin has a significant amount of serine in the amino acid composition of about 37 mol%, and is characterized by gelling when dissolved in hot water and cooled. In addition, studies by the inventors revealed that the tyrosinase inhibitory activity, water absorption and viscosity are very excellent.

종래 미용팩에 사용되는 화장료의 성분은 대개 인공으로 합성한 조성물을 사용하는 경우가 대부분이어서 연약한 피부에 손상을 주거나 건강하고 윤택한 피부로 재생시키는 효과는 뒤떨어지는 실정이다. 또한, 인공합성 미용팩 조성물은 심한 경우 피부발진이나, 알레르기 등의 부작용을 일으킬 수 있는 매우 심각한 단점을 갖고 있다. 따라서, 최근에는 미용팩 제품에 보습효과가 있고 미백효과가 우수한 천연물을 이용하려는 시도가 증가하고 있다. 국내공개특허 제2003-0039389는 황백, 구기자, 녹두, 금은화, 백봉령, 약쑥, 당귀, 백감장, 율무, 감초, 고삼, 상백피, 황금, 은행잎, 백금, 하수오, 백렴, 송진, 영지 버섯, 로얄제리, 꿀, 율피(栗皮), 나팔꽃 씨, 우방자를 각각 동일한 무게비율로 배합하고 끓여서 얻은 추출액과 국화와 배를 배합하고 이를 끓여서 얻은 추출액을 1:1로 혼합하여서 얻은 50중량％의 추출액과, 콜라겐산, 히아룬산, 알긴산 혼합액 20중량％와, 제얄제리 및 꿀 혼합물 20중량％와, 송진 10중량％를 함유하는 한방 및 천연추출물을 포함하는 미용팩용 화장료 조성물을 개시하고 있다.The components of the cosmetics used in the conventional cosmetic packs are mostly made of artificially synthesized compositions, so that the effects of damaging or regenerating healthy skin are inferior. In addition, artificial synthetic cosmetic pack composition has a very serious disadvantage that can cause side effects such as skin rashes, allergies in severe cases. Therefore, in recent years, attempts to use natural products having a moisturizing effect and an excellent whitening effect on cosmetic pack products have been increasing. Korean Patent Laid-Open No. 2003-0039389 discloses baekbaek, gojija, mung bean, gold and silver, baekbongyeong, wormwood, donkey, baekgamjang, yulmu, licorice, red ginseng, baekbaekpi, golden, ginkgo biloba, platinum, sewage, baekryeom, pine needles, ganoderma lucidum 50% by weight of an extract obtained by mixing honey, yulpi, morning glory seeds, and allies in the same weight ratio, by mixing boiled extracts with chrysanthemums and pears and mixing them in a 1: 1 ratio, A cosmetic composition for cosmetic packs comprising 20% by weight of a mixture of collagen, hyaluronic acid, and alginic acid, 20% by weight of zeyal jelly and honey mixture, and 10% by weight of rosin is disclosed.

그러나, 현재 상기와 같은 천연물들은 어느 정도의 피부미백효과는 기대할 수 있지만, 수분흡수력 및 점도의 측면에서는 그리 만족할 만한 특성을 발휘하지 못하고 있는 실정이어서 이에 대한 강화가 요구되고 있다. However, at present, such natural products can be expected to a certain degree of skin whitening effect, but in terms of water absorption capacity and viscosity does not exhibit such a satisfactory characteristic is required to strengthen it.

본 발명은 상기 종래기술이 가지는 문제를 해결하기 위해 안출된 것으로, 그 목적은 티로시나아제 저해활성과 수분흡수성과 같은 우수한 특성을 가진 세리신 단백질에 각종 천연물을 첨가하여 피부미용팩의 기능을 강화시킨 미용팩 조성물을 제공하는데 있다.The present invention has been made to solve the problems of the prior art, its purpose is to enhance the function of the skin care pack by adding various natural products to sericin protein having excellent properties such as tyrosinase inhibitory activity and water absorption To provide a composition.

상기 목적을 달성하기 위하여 본 발명은 누에고치로부터 추출한 세리신을 활성성분으로 함유하는 피부미용팩 조성물을 제공한다. In order to achieve the above object, the present invention provides a skin care pack composition containing sericin extracted from cocoon as an active ingredient.

바람직하게는 세리신은 누에고치의 열수추출물을 동결건조한 분말상으로 첨가하는 것이 좋다.Preferably, sericin is preferably added to the lyophilized powder of hot water extract of cocoon.

또한 본 발명의 피부미용팩 조성물은 포도씨분말, 살구씨분말, 율무분말, 감자분말, 녹차분말, 밤껍질분말, 송이버섯, 살구씨분말, 레몬즙, 레몬즙, 비타민 C, 레몬향의 군으로부터 선택되는 적어도 하나 이상의 성분을 더 포함할 수 있다.In addition, the skin care pack composition of the present invention is selected from the group of grape seed powder, apricot seed powder, yulmu powder, potato powder, green tea powder, chestnut powder, pine mushroom, apricot seed powder, lemon juice, lemon juice, vitamin C, lemon flavor It may further comprise at least one or more components.

이하, 본 발명의 내용을 구체적인 제조예를 들어 보다 상세하게 설명하고자 한다.Hereinafter, the present invention will be described in more detail with reference to specific production examples.

누에고치로부터 세리신을 추출하는 과정은 공지의 다양한 추출방법을 통해 달성되어질 수 있어 특별히 제한되지는 않는다. 따라서, 세리신의 추출과정은 열수추출 및 용매추출법 등 다양한 방법이 사용될 수 있다. 이하에서는, 세리신의 추출과정으로서 열수추출방법을 개시한다. 다만, 당업자라면 본 발명의 권리범위는 여기에 제한되어지지 않음을 용이하게 이해할 수 있을 것이다. The process of extracting sericin from the cocoon can be achieved through various known extraction methods, and is not particularly limited. Therefore, the extraction process of sericin can be used a variety of methods, such as hot water extraction and solvent extraction. Hereinafter, a method of extracting hot water as an extraction process of sericin is disclosed. However, those skilled in the art will readily understand that the scope of the present invention is not limited thereto.

세리신의 추출과정은 도 1에 도시된 바와 같다. 먼저, 누에고치 출각현을 음용수에 세척하고 이것을 바람직하게는 80∼120℃, 보다 바람직하게는 100℃ 정도로 가열한 열수에 특별히 제한하는 것은 아니며, 대략 2∼4중량％ 정도가 되도록 첨가하여 30분 정도로 끓여 제조한다. 상기 과정을 통해 얻은 추출물은 거즈로 여과하고 이로부터 세리신 단백질을 추출한 후 이를 동결건조하여 분말로 제조한다.Extraction process of sericin is as shown in FIG. First, the cocoon spinach is washed with drinking water, and preferably it is not particularly limited to hot water heated to 80 to 120 ° C, more preferably to 100 ° C, and added to be about 2 to 4% by weight for 30 minutes. Boil to the extent of manufacture. The extract obtained through the above process is filtered with gauze and extracted from the sericin protein and then lyophilized to prepare a powder.

상기 과정을 거쳐 얻어진 세리신은 수분흡수력과 점도 특성이 좋으며, 티로시나아제 저해활성이 우수하여 단독으로 미용팩 조성물로 첨가될 수 있는 것은 물론이며, 기존에 피부미백효과가 있다고 알려진 다양한 천연추출물 등과도 혼합하여 첨가될 수 있다. 후자의 경우에는 기존에 알려진 각 천연추출물의 피부미백작용과 상승적으로 작용하여 더욱 우수한 피부미백효과를 발휘할 수 있다. 함께 사용가능한 천연추출물의 종류는 특별한 한정을 요하지는 아니한다. 따라서, 인체에 안전하게 사용할 수 있으며, 피부미백효과를 가지는 어떠한 천연 추출물도 함께 첨가될 수 있다. 이러한, 천연추출물의 예로는, 황백, 구기자, 녹두, 금은화, 백봉령, 약쑥, 당귀, 백감장, 율무, 감초, 고삼, 상백피, 황금, 은행잎, 백금, 하수오, 백렴, 송진, 영지 버섯, 로얄제리, 꿀, 율피(栗皮), 나팔꽃 씨, 우방자 등의 추출물도 있지만, 바람직하게는 포도씨분말, 살구씨분말, 율무분말, 감자분말, 녹차분말, 밤껍질분말, 송이버섯, 살구씨분말, 레몬즙, 레몬즙, 비타민 C, 레몬향의 군으로부터 선택되는 적어도 하나 이상의 성분을 사용하는 것이 보다 좋다.The sericin obtained through the above process has good water absorption and viscosity characteristics, and has excellent tyrosinase inhibitory activity, so that it can be added alone as a cosmetic pack composition, and also mixed with various natural extracts known to have skin whitening effects. Can be added. In the latter case, it is possible to exert a more excellent skin whitening effect by synergistically acting with the skin whitening action of each known natural extract. The types of natural extracts that can be used together do not require special limitations. Therefore, it can be safely used in the human body, and any natural extract having a skin whitening effect can be added together. Examples of such natural extracts, yellow white, wolfberry, mung bean, gold and silver, baekbongryung, wormwood, donkey, baekgamjang, yulmu, licorice, red ginseng, baekbaekpi, golden, ginkgo biloba, platinum, sewage, baekryeom, rosin, ganoderma lucidum, royal jelly Also extracts such as honey, yulpi, morning glory seeds, allies, but preferably grape seed powder, apricot seed powder, yulmu powder, potato powder, green tea powder, chestnut powder, matsutake mushroom, apricot seed powder, lemon It is better to use at least one component selected from the group consisting of juice, lemon juice, vitamin C, lemon scent.

본 발명에 따른 바람직한 실시예로서 미용팩 제품은 정제수 58.15중량％에 세리신 단백질 분말 2.0중량％와 한천 0.15중량％를 녹여 80도에서 10분간 가열하고 식힌 후, 녹차분말 0.7중량％, 밤껍질분말 6중량％, 살구씨분말 5중량％, 율무분말 10중량％, 송이버섯 1중량％, 레몬즙 15중량％, 비타민 C 1중량％, 레몬향 1중량％를 첨가하고 잘 혼합하여 제조할 수 있다.As a preferred embodiment of the present invention, the cosmetic pack product was dissolved in 2.0.% By weight of sericin protein powder and 0.15% by weight of agar in 58.15% by weight of purified water, heated at 80 ° C. for 10 minutes, and cooled. %, 5% by weight of apricot seed powder, 10% by weight of radish powder, 1% by weight of pine mushroom, 15% by weight of lemon juice, 1% by weight of vitamin C, and 1% by weight of lemon flavor can be added and mixed well.

이하 미용팩의 실시예를 들어 보다 구체적으로 설명하지만 본 발명이 이들 예로만 한정되는 것은 아니다.Hereinafter, the present invention will be described in more detail with reference to examples of cosmetic packs, but the present invention is not limited only to these examples.

<실시예 1, 2> 세리신 추출물의 제조Example 1, 2 Preparation of Sericin Extract

세리신 추출물은 하기 표 1의 조성에 준하여 도 1과 같은 공정에 따라 누에고치로부터 세리신 단백질을 추출한 후 동결건조하여 분말화하여 제조하였다.Sericin extract was prepared by extracting sericin protein from silkworm cocoon according to the composition of Table 1 and then lyophilized and powdered.

<표 1>TABLE 1

성분ingredient 조성(중량％)Composition (% by weight) 실시예 1Example 1 누에고치 출각현음용수Cocoon appearance 298298 실시예 2Example 2 누에고치 출각현음용수Cocoon appearance 496496

<시험예 1><Test Example 1>

세리신 단백질의 티로시나아제 저해활성을 측정한 결과 하기 표 2에서와 같이 누에고치는 세리신 무첨가구에 비해 첨가구에서 약 2배의 티로시나아제 활성이 저해되는 것으로 나타났다. 이러한 결과로부터 세리신은 멜라닌 색소형성을 억제하는 미백효과가 상당히 뛰어난 것으로 판단되며 피부미백 화장제품으로서의 사용도 가능할 것으로 판단된다. As a result of measuring the tyrosinase inhibitory activity of the sericin protein, silkworm cocoon was shown to inhibit about 2 times tyrosinase activity in the added group as compared to the sericin-free group as shown in Table 2 below. From these results, sericin is considered to have a very good whitening effect of inhibiting melanin pigmentation and may be used as a skin whitening cosmetic product.

<표 2>TABLE 2

세리신 첨가량 (％)Sericin added amount (%) 티로시나아제 활성(흡광도/분)Tyrosinase activity (absorbance / min) 0.00.0 0.0850.085 0.50.5 0.0650.065 1.01.0 0.0420.042

<시험예 2><Test Example 2>

세리신 단백질의 수분흡수력을 조사하기 위해 누에고치의 50배 분량의 물에 고치를 넣고 100℃에서 30분간 가열하여 세리신을 추출한 용액 (약 70℃)에 각종 섬유를 침지시킨 다음 50℃의 송풍건조기를 이용하여 48시간 완전히 건조시킨 후, low temperature humidity chamber KHC-OC(500×600 mm)를 이용하여 송풍량 85W, 상대습도 80, 온도 30℃에서 3시간, 6시간 경과 후의 각종 섬유의 무게를 측정하여 보습정도를 측정하였다. 그 결과 하기 표 3에서와 같이 수부흡수력은 A > E > B > C = D 인 것으로 나타나, 평균 2.33중량％의 수분이 흡수되는 것으로 나타났다. 따라서, 수분흡수력을 인공섬유를 대상을 측정한 결과이지만 세리신 추출물이 섬유의 수분흡수율을 증가시키는데 중요한 역할을 한다는 것으로 나타나, 수분보유력을 필요로 하는 미용팩에의 적용성은 매우 높은 것으로 판단된다.To investigate the water absorption of sericin protein, put cocoons in 50 times the amount of silkworm cocoons and heat them at 100 ° C for 30 minutes to immerse various fibers in a solution (about 70 ° C) extracted from sericin, and then blow air at 50 ° C. After drying completely for 48 hours using a low temperature humidity chamber KHC-OC (500 × 600 mm), the weight of various fibers after 3 hours and 6 hours at 85W, relative humidity 80 and temperature 30 ℃ was measured. Moisturizing degree was measured. As a result, as shown in Table 3, the water absorption was found to be A> E> B> C = D, which was found to absorb 2.33% by weight of water on average. Therefore, the results of measuring the water absorption of artificial fibers, but the sericin extract appears to play an important role in increasing the water absorption of the fiber, it is judged that the applicability to the beauty pack that requires water retention capacity is very high.

<표 3>TABLE 3

섬 유fiber 섬유무게(g)Fiber weight (g) 끓인 물Boiled water 세리신 추출물Sericin Extract 3시간(증가율％)3 hours (% increase) 6시간(증가율％)6 hours (% increase) 3시간(증가율％)3 hours (% increase) 6시간(증가율％)6 hours (% increase) A, 폴리에스테르 ＋스판A, polyester + span 2.01552.0155 2.0567(102.0)2.0567 (102.0) 2.0979(104.1)2.0979 (104.1) 2.1052(104.5)2.1052 (104.5) 2.1305(105.7)2.1305 (105.7) B, 아세테이트50%＋ 레이온50%B, 50% of acetate + 50% of rayon 2.22892.2289 2.2451(100.7)2.2451 (100.7) 2.2613(101.5)2.2613 (101.5) 2.2787(102.2)2.2787 (102.2) 2.3051(103.4)2.3051 (103.4) C, 면＋스판C, cotton + span 2.34252.3425 2.3515(100.4)2.3515 (100.4) 2.3603(100.8)2.3603 (100.8) 2.4055(102.7)2.4055 (102.7) 2.4158(103.1)2.4158 (103.1) D, 면＋폴리우레탄D, cotton + polyurethane 2.41642.4164 2.4242(100.3)2.4242 (100.3) 2.4322(100.7)2.4322 (100.7) 2.4474(101.3)2.4474 (101.3) 2.4905(103.1)2.4905 (103.1) E, 삼베E, burlap 3.29143.2914 3.3156(100.7)3.3156 (100.7) 3.3399(101.5)3.3399 (101.5) 3.3165(100.8)3.3165 (100.8) 3.4186(103.9)3.4186 (103.9)

증가율 : 섬유무게를 100으로 하였을 때의 환산치 Increase rate: Conversion value when the fiber weight is 100

<시험예 3><Test Example 3>

세리신 단백질 추출물이 점성을 가지는 성질이 어느 정도인지를 조사하기 위하여 실시예 1과 2와 같은 조성으로 추출물을 제조한 후, 4℃에서 50℃의 범위에서 점도를 측정하였다. 그 결과 도 2에서와 같이 누에고치 4％ 추출물의 경우 2％ 추출물 보다 약 2배 정도의 점도가 있었으며 또한 온도가 낮아질수록 점성이 비례적으로 높아지는 경향을 확인할 수 있었다. 이러한 성질로부터 세리신 단백질은 미용팩 제조시 점성부여에 크게 기여할 수 있을 것으로 판단된다. In order to investigate how much the sericin protein extract has a viscous property, the extract was prepared in the same composition as in Examples 1 and 2, and the viscosity was measured in the range of 4 ° C to 50 ° C. As a result, as shown in FIG. 2, the silkworm cocoon 4% extract had a viscosity about 2 times higher than the 2% extract, and the viscosity was increased in proportion to the temperature. From these properties, sericin protein is considered to be a great contributor to the viscosity in the manufacture of cosmetic packs.

<실시예 3> 미용팩의 제조Example 3 Preparation of Cosmetic Pack

미용팩은 하기 표 4의 실시예 3과 같은 조성으로 정제수에 세리신 분말과 한천을 녹여 80℃에서 10분간 가열하여 식힌 후, 녹차분말, 밤껍질분말, 살구씨분말, 율무분말, 송이버섯, 레몬즙, 비타민 C, 레몬향을 첨가혼합하는 과정을 통해 제조되었다.The cosmetic pack is dissolved in sericin powder and agar in purified water in the same composition as in Example 3 of Table 4, and then cooled by heating at 80 ° C. for 10 minutes, followed by green tea powder, chestnut powder, apricot seed powder, yulmu powder, pine mushroom, and lemon juice. It is prepared by adding and mixing vitamin C and lemon scent.

<표 4>TABLE 4

성분ingredient 조성(중량％)Composition (% by weight) 실시예 3Example 3 세리신 동결건조분말한천녹차분말밤껍질분말살구씨분말율무분말송이버섯레몬즙비타민 C레몬향정제수Sericin Freeze-Dried Powder Agar Green Tea Powder Chestnut Peel Powder Apricot Seed Powder Rate Powder Powder Mushroom Mushroom Lemon Juice Vitamin C Lemon 2.00.150.76.05.010.01.015.01.02.556.752.00.150.76.05.010.01.015.01.02.556.75

<시험예 4> 품질특성평가Test Example 4 Evaluation of Quality Characteristics

실시예 3의 조성비로 미용팩을 제조하여 20∼40대 여성 20명을 대상으로 임상시험을 실시한 후 설문조사를 실시하였다. 설문조사의 항목은 1. 본 팩제품의 향, 2. 팩을 얼굴에 발랐을 때의 발라잠성, 3. 팩제품을 사용한 후의 느낌, 4. 팩 제품을 사용하고 난 후의 피부상태의 4가지로 하였다. A cosmetic pack was prepared at the composition ratio of Example 3, and a questionnaire was conducted after conducting a clinical trial of 20 women in their 20s to 40s. The questionnaire was divided into four categories: 1. The fragrance of the pack product, 2. The balsamism when the pack was applied to the face, 3. The feeling after using the pack product, and 4. The skin condition after using the pack product. .

그 결과, 하기 표 5에서와 같이 향의 경우 보통 이상이라고 평가한 여성이 약 50％였으며 팩의 발라짐성은 평가 여성의 75％가 좋고, 8.3가 매우 좋다고 평가하였으며 팩 사용후의 느낌에 대해서는 58.3％가 좋았고, 8.3％가 매우 좋은 것으로 평가하였다. 사용 후 피부상태에 대한 답변에서는 50％가 좋아졌고, 16％가 매우 좋아졌다고 평가하여 본 제품의 미용팩으로서의 효용성이 상당히 높은 것으로 평가되었다. 이러한 임상시험 결과로부터 세리신 단백질의 피부미용팩의 원료로서 적합한 것으로 판단된다.As a result, as shown in Table 5, about 50% of women evaluated normal fragrance in terms of fragrance, and the baldness of the pack was evaluated as 75% of the evaluated women, 8.3 was very good, and 58.3% of the feeling after using the pack. Was good and 8.3% evaluated it as very good. In response to skin condition after use, 50% was improved and 16% was very good, indicating that the efficacy of this product as a beauty pack was considerably high. From these clinical trial results, it is judged to be suitable as a raw material of skin beauty pack of sericin protein.

<표 5>TABLE 5

향(％)incense(%) 발라짐성(％)Spreadability (%) 느낌(％)feeling(%) 사용후 피부상태 (％)Skin condition after use (%) 매우 좋다very good 00 8.38.3 8.38.3 16.416.4 좋다good 8.38.3 75.075.0 58.358.3 50.050.0 그저 그렇다so so 41.741.7 16.716.7 16.416.4 33.333.3 나쁘다bad 25.025.0 00 16.716.7 00 매우 나쁘다Very bad 25.025.0 00 00 00

본 발명은 티로시나아제 저해활성 효과가 있는 누에고치 세리신 추출분말을 활성성분으로 하고 여기에 한천과 티로시나아제 저해활성 또는 항산화활성이 있는 각종 첨가물로서 녹차분말, 밤껍질분말, 살구씨분말, 율무분말, 송이버섯, 레몬즙, 비타민 C, 레몬향 등의 첨가물을 혼합하여 피부미백효과가 있는 미용팩을 제조할 수 있다. 이에 따라, 누에고치의 활용성를 증진시켜 누에 부산물인 고치의 부가가치를 향상시켜 양잠농가의 소득증진과 누에 종자보존사업에 기여하고 제품에 기능성을 부여하여 국민건강에 크게 기여할 것으로 예상된다.The present invention is a cocoon sericin extract powder having a tyrosinase inhibitory activity as an active ingredient and agar and tyrosinase inhibitory activity or antioxidant additives as various additives green tea powder, chestnut powder, apricot seed powder, yulmu Powder, Matsutake mushroom, lemon juice, vitamin C, lemon flavorings can be mixed to produce a beauty pack with skin whitening effect. Accordingly, it is expected that the utilization of silkworm cocoon will be improved, thereby improving the added value of cocoon, a by-product of silkworm, thereby contributing to the increase of income for sheep farmers and the silkworm seed preservation project, and contributing functionality to the product.

이상에서 본 발명은 기재된 실시예에 대해서만 상세히 설명되었지만 본 발명의 기술사상 범위 내에서 다양한 변형 및 수정이 가능함은 당업자에게 있어서 명백한 것이며, 이러한 변형 및 수정은 첨부된 특허청구범위에 속함은 당연한 것이다.Although the present invention has been described in detail only with respect to the embodiments described, it will be apparent to those skilled in the art that various modifications and variations are possible within the technical scope of the present invention, and such modifications and modifications are within the scope of the appended claims.

도 1은 누에고치로부터 세리신 단백질을 추출하는 과정의 공정흐름도 1 is a process flow diagram of the process of extracting sericin protein from cocoon

도 2는 본 발명에 따라 추출한 세리신 단백질의 점성 측정 결과그래프Figure 2 is a graph of the viscosity measurement results of sericin protein extracted according to the present invention

Claims (3)

누에고치로부터 추출한 세리신을 활성성분으로 함유하는 피부미용팩Skin care pack containing sericin extracted from cocoon as active ingredient 제1항에 있어서, 세리신은 누에고치의 열수추출물을 동결건조한 분말상으로 첨가됨을 특징으로 하는 피부미용팩The skin care pack according to claim 1, wherein sericin is added to the lyophilized powder of hot water extract of cocoon. 제1항 또는 2항에 있어서, 포도씨분말, 살구씨분말, 율무분말, 감자분말, 녹차분말, 밤껍질분말, 송이버섯, 살구씨분말, 레몬즙, 레몬즙, 비타민 C, 레몬향의 군으로부터 선택되는 적어도 하나 이상의 성분을 포함함을 특징으로 하는 피부미용팩The method according to claim 1 or 2, wherein from the group of grape seed powder, apricot seed powder, yulmu powder, potato powder, green tea powder, chestnut powder, matsutake, apricot seed powder, lemon juice, lemon juice, vitamin C, and lemon flavor Skin care pack, characterized in that it comprises at least one component selected
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US9359401B2 (en) 2011-05-23 2016-06-07 Incospharm Corporation Peptide analogues with an excellent moisturizing effect and use thereof
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CN108354881A (en) * 2018-04-16 2018-08-03 浙江省农业科学院 A kind of thermophilic curry facial mask and preparation method thereof of Phellinus and sericin combination
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