KR101831756B1 - Cosmetic composition for improving wrinkles and the patch for the skin around eyes using the same - Google Patents

Abstract

The present invention relates to a cosmetic composition for improving skin elasticity and improving wrinkles and improving moisture retention, and a patch for an eye patch containing the same. More particularly, the present invention relates to a cosmetic composition which contains a rose extract of Bulgaria and thereby enhances the biosynthesis of procollagen to provide an effect of improving wrinkles, improving the elasticity and improving the moisturizing effect, Lt; / RTI >

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition for improving wrinkles and a patch for an eye skin using the composition.

The present invention relates to a cosmetic composition for enhancing skin elasticity and improving wrinkles and moisturizing power by containing Bulgarian rose extract as an active ingredient, and a patch for an eye patch containing the same.

Roses have become an indispensable ingredient in cosmetics due to the rich nutrients of roses. Especially, the rose bud contains sugar, amino acid, vitamin C, tannin and citric acid, so it is excellent for skin moisturizing, astringent and cleansing, and also for deep moisturizing and anti-aging for dry skin and sensitive skin. The roses harvested in April and May are considered to be the best, and the roses harvested when the buds are in bloom are more effective than the ones blooming. In addition, the quality of roses varies according to the area and cultivation method, and most Bulgarians are recognized as the highest grade.

Other flowers have been loved by many women, but flowers that are deeply involved in the life and beauty of women are rare. In ancient Egypt, Cleopatra enjoyed a bath of roses, and in Europe it was used as a source of lotion or as a treatment for burns and burns. In addition, even when perfume is produced, fragrance to which roses are not added is almost invisible. A variety of cosmetics based on natural ingredients have emerged in the well-being and naturalistic craze, but among them, cosmetics containing rosin is the most popular.

The present inventors have found that a rose of the present invention has an antioxidative and moisturizing effect and has an effect of increasing the biosynthesis of procollagen at a specific concentration or higher, and is applied to a composition capable of improving the wrinkles of the skin, Respectively.

Accordingly, it is an object of the present invention to provide a cosmetic composition which is effective for improving skin wrinkles.

It is another object of the present invention to provide an effective patch for eye care by impregnating the composition.

In order to achieve the above object, the present invention provides an anti-aging cosmetic composition comprising a Bulgarian rose extract in an amount of 0.1 to 10.0% by weight based on the total weight of the composition.

The present invention also relates to Patch 1, a melting film containing acetylglucosamine and adenosine; And a patch 2 impregnated with a composition containing a Bulgarian rose extract.

The composition of the present invention is effective for improving wrinkles and elasticity and improving moisturizing power. In particular, it can improve the skin problem of the eye area when applied to the eye area. In addition, when using the patch containing it, the skin of the eye can be conveniently managed.

Figure 1 shows an exemplary form of a patch according to the invention consisting of Patch 1 and Patch 2.
Fig. 2 shows the results of measuring antioxidant efficacy of Bulgarian rose extract.
Figure 3 shows the effect of hyaluronic acid synthesis of Bulgarian rose extract, measured by ELISA analysis based on the specific binding of hyaluronic acid to hyaluronic acid (HA) binding protein.
Fig. 4 shows the results of measuring the effect of increasing the prolactin biosynthesis of Bulgarian rose extract.
5 shows the application of the patches 1 and 2 to the face.
6 shows a visual evaluation result of the wrinkle improvement degree by a specialist (0w: initial, 0w ': immediately after use, 3w: 3 weeks, 6w: 6 weeks).
FIG. 7 shows the result of measuring the recovery force of the eye wrinkles using the patch of the present invention caused by wrinkles of the eyes. (0w: initial, 0w ': immediately after use, 3w: 3 weeks, 6w: 6 weeks).
FIG. 8 shows the results of evaluating the degree of wrinkle improvement perceived by subjects while using the patch of the present invention (0 w: initial, 3 w: 3 weeks, 6 w: 6 weeks).
FIG. 9 shows the measurement results of the amount of skin moisture in the eyes according to the use of the patch of the present invention (0w: first, 0w ': immediately after use, 3w: 3 weeks).
Fig. 10 shows changes in the wrinkles of the skin before and after use of the patch of the present invention.
11 shows the degree of improvement in eye elasticity, degree of improvement in eye wrinkles and satisfaction (3 w: 3 wks, 6 w: 6 wks) after 3 weeks and 6 weeks after using the patch of the present invention.

The present invention provides a composition capable of improving the skin condition by containing a Bulgarian rose extract as an active ingredient. In particular, the composition improves skin elasticity to improve wrinkles and improve moisturizing power.

The Bulgarian rose extract used in the present invention is not limited to this, but preferably it is extracted from a flower bud having a calyx and petal buds in a state just before flowering. The Bulgarian rose extract of the present invention can be prepared from Bulgarian roses using an extraction method commonly used in the art. Preferably, ultra high pressure technology can be used among the non-heat treatment methods. Extra-high pressure extraction method extracts a useful ingredient by instantaneously exploding a cell membrane by treating thousands of bar pressure without using gas or heat at all, so that the extraction efficiency is high and natural nutrients which are not processed at all can be obtained.

The cosmetic composition of the present invention contains the Bulgarian rose extract in an amount of 0.1 to 10.0% by weight based on the total weight of the composition. If the content is less than 0.1% by weight, the effect is insufficient. If the content is more than 10.0% by weight, cytotoxicity to the melan-a cell line is exhibited.

The composition of the present invention is not particularly limited in its formulation depending on the body part to be applied. Specifically, it may be, for example, a cosmetic composition having a formulation of a softening agent, a nutritional lotion, a massage cream, a nutritional cream, an eye cream, a pack, a gel or a skin adhesive type cosmetic composition and may also be a lotion, ointment, gel, cream, And may be a transdermal dosage form such as a spray.

In addition, in the cosmetic composition according to each formulation, other components other than the above-described Bulgarian rose extract of the present invention may be mixed and selected without difficulty by those skilled in the art depending on the purpose of formulation or use, etc. In this case, A synergistic effect can occur.

In addition, the present invention provides a patch that facilitates skin care for the eyes.

Specifically, the patch of the present invention comprises patch 1, which is a melting film, and patch 2, which promotes the absorption of components contained in patch 1. An exemplary form thereof is shown in Fig.

Patch 1:

Patch 1 is a boomerang dish-shaped melting film that is applied to maximize the improvement of wrinkles and elasticity. The shape is designed to effectively deliver the components contained in patch 1.

Patch 1 contains acetylglucosamine and adenosine as potent components. Acetylglucosamine is contained in an amount of 0.1 to 5.0% by weight based on the total weight of the patch 1, and adenosine is contained in an amount of 0.01 to 5.0% by weight based on the total weight.

In order to form a film, pullulan is contained in an amount of 1.0 to 60.0% by weight based on the total weight of the patch 1, and the carob bean gum and carrageenan are contained in an amount of 0.01 to 1.0% by weight as a thickening agent.

Patch 2:

Patch 2 promotes the absorption of the components of patch 1, and promotes wrinkle, elasticity and moisturization by managing the entire skin area of the eyes. Patch 2 becomes a panda shape when applied to facial skin.

The composition impregnated in Patch 2 contains a Bulgarian rose extract as an effective ingredient and the Bulgarian rose extract is contained in an amount of 0.1 to 10.0% by weight based on the total weight of the composition impregnated in Patch 2. If the content is less than 0.1% by weight, the effect is insufficient. If the content is more than 10.0% by weight, cytotoxicity to the melan-a cell line is exhibited.

The patch 2 can be manufactured by using a material generally used for a mask pack sheet, and for example, natural materials, net roots, hydrogels, silicone gels and the like can be used. Patch 2 is applied by impregnating a water-soluble cosmetic composition, and the composition of an exemplary water-soluble cosmetic composition that can be used at this time is shown in Table 2.

Since the patches for patches 1 and 2 of the present invention are different from each other in the patches for patches 1 and 2 of the present invention, they can be more effectively used for skin care. Specifically, the patch of the present invention can be applied in such a manner that the patch 1 is attached to the subcutaneous skin and the patch 1 is covered with the patch 2.

Also, in order to obtain a higher level of improvement, apply the above-mentioned eye cream, apply patch 1 to the under-eye area desired for intensive care, lightly tap on the patch 1, and hold the patch 1. After about 1 to 2 minutes, 2 can be used for 20 minutes by wetting the eyes sufficiently.

The present invention also provides eye cream formulations so as to obtain a higher eye wrinkle-improving effect of the patch for the skin of the eyes.

The eye cream is used in combination with a patch to provide a synergistic effect of wrinkle improvement. Although effective ingredients that can be effective for improving wrinkles are not particularly limited, use of EGCG or peptiskin as an active ingredient Can be used.

Epigallocatechin gallate (EGCG) is a substance that inhibits oxidation by active oxygen in vivo when applied to the skin, inhibits the production of peroxide to prevent aging of the biological membrane, and has an effect of promoting collagen biosynthesis Which is not only irritating to the skin but also has an effect of preventing skin aging and improving skin elasticity. In addition, peptiskin is an effective substance for enhancing alveolar enhancement, which promotes biosynthesis of collagen upon application to the skin and inhibits collagen degradation and cross linking of collagen.

The eye cream provided for use with the patch of the present invention is not limited thereto, but preferably has the following composition. The eye cream may contain 0.001 to 10% by weight of EGCG and 0.001 to 10% by weight of peptiskin, based on the total weight of the eye cream composition.

The eye cream is a branched polymer having a lipophilic alkyl chain as a side chain, and may be an acrylate / C10-30 alkyl acrylate crosspolymer, an acrylate / C12-22 alkyl methacrylate copolymer (Acrylates / C12-22 Alkyl Methacrylate Copolymer (Acrylates / Beheneth-25 Methacrylate Copolymer) or an acrylate / Ceteth-20 Methacrylate Copolymer (acrylate / Ceteth-20 Methacrylate Copolymer) Most preferably an acrylate / C10-30 alkyl acrylate crosspolymer is used. Silicone oils such as dimethicone or cyclomethicone which are generally used in the production of cosmetics; Ester-based oils such as cetyl ethyl hexanoate or C12-15 alkyl benzoate; Hydrocarbonated oils such as Hydrogenated Polydecene or Squalane, and mixtures thereof may be contained in an amount of 2 to 30% by weight based on the total weight of the composition, but are not limited thereto.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the following examples are intended to illustrate the present invention, but the scope of the present invention is not limited thereto.

[Reference Example 1] Preparation of Bulgarian rose extract

Bulgarian rose extract was prepared by directly importing Bulgarian Damascena Rose Bud from a cultivated farm in the Kazanlak region, famous for its Bulgarian rose valley. The Bulgarian rose bud used in the present invention was in a state in which the calyx and flower petals were gathered together just before the flowering.

Bulgarian rose buds were homogenized directly into a motorized homogenizer. Occasionally bead beaters were used to dissolve hard plant cells. This was incubated in cold water for 24 hours. Water-soluble fractions were then extracted for 1 hour at room temperature while vortexing. After centrifugation at 15,000 xg for 1 hour at room temperature, the insoluble material was discarded and the soluble portion was used to make cosmetic ingredients.

[Reference Example 2] Preparation of cosmetic ingredients

The Bulgarian rose bud extract obtained in Reference Example 1 was diluted to an appropriate concentration with 30% (v / v) propanediol. The diluted product was then incubated with stirring for 6 hours and filtered through a membrane filter with a diameter of 0.2 mu m.

[Test Example 1] Antioxidant efficacy of Bulgarian rose extract

DPPH assay was used to measure the antioxidant capacity of Bulgarian rose extract. 1,1-Diphenyl-2-picrylhydrazyl (DPPH) is a very stable free radical, a violet compound that exhibits characteristic light absorption at 520 nm. Radicals are very stable in organic solvents such as alcohols, and especially because they are decolorized by proton-radical scavengers among various antioxidant mechanisms, the antioxidant activity can be easily observed with the naked eye.

First, 1 mM DPPH solution was prepared. Then, a sample of the Bulgarian rose extract of Reference Example 1 was dissolved in ethanol by concentration, and the DPPH solution and the sample were mixed at a ratio of 4: 1. After incubation for about 30 minutes, the absorbance was measured at 520 nm. As a positive control, ascorbic acid, an excellent antioxidant, was used. The measurement results are shown in Fig.

2, the Bulgarian rose extract showed an antioxidative activity of about 90% against ascorbic acid, which is an excellent antioxidant, at an EC50 of about 1.5% and a concentration of 5% or more. Able to know.

[Test Example 2] Measurement of moisturizing effect of Bulgarian rose extract

The moisturizing power of Bulgarian rose extract was evaluated by the amount of hyaluronic acid secreted by human keratinocytes.

Concretely, human keratinocyte was inoculated into a 48-well plate at 1 * 10 ⁴ cells / well and cultured. Samples were then treated with 0.0%, 0.1%, 0.5% and 1.0% of Bulgarian rose extract of Reference Example 1. After culturing for 72 hours, the amount of hyaluronic acid, which is a moisture enhancing substance secreted to the medium, was measured by ELISA analysis. The measurement results are shown in Fig.

3 shows that the Bulgarian rose extract increases the biosynthesis of hyaluronic acid present in the culture supernatant by about 20% at a concentration of 1%, thereby improving the moisturizing power of the Bulgarian rose extract when applied to the skin It can be judged to be possible.

[Test Example 3] Measurement of the increase effect of procollagen biosynthesis of Bulgarian rose extract

The skin elasticity enhancement effect of Bulgarian rose extract was evaluated by the amount of flow collagen secreted by human fibroblast.

Specifically, human fibroblast HDF cells were inoculated into a 48-well plate at 1 × 10 ⁴ cells / well and cultured. Samples were treated with 0.01%, 0.1% and 1.0% of Bulgarian rose extract of Reference Example 1. After 72 hours of culture, the amount of procollagen secreted into the medium was measured by ELISA analysis. The measurement results are shown in Fig.

4, it can be concluded that Bulgarian rose extract has a significant effect of increasing the production of procollagen biosynthesis at a concentration of 1% or more, which is effective for improving skin wrinkles.

[Reference Example 3] Production of eye patch

Manufacture of Patch 1

Patch 1 was prepared with the composition shown in Table 1 below.

Patch 1 Component (content,% by weight) Example Pullulan 52 Trehalose 17 Jojoba seed oil 13 Propylene glycol 1.6 glycerin 1.3 Polysorbate 60 1.1 Hydrogenated starch hydrolyzate 0.9 Acetylglucosamine 0.53 Spices 0.3 Sorbitan stearate 0.3 Serratonia silyqua gum 0.2 Carrageenan 0.2 Hydrolyzed collagen 0.2 Xanthan gum 0.1 Adenosine 0.04 water To 100

A concrete production method of the patch 1 is as follows.

The components shown in Table 1 were mixed while stirring at room temperature in a heating vessel. First, pullulan was added to ionized water, and the temperature of the solution was raised to 55 ° C or higher, and the remaining materials were added while maintaining the temperature of the solution at 55 ° C or higher. The thickener and the potentiator were added later to prepare Patch 1 according to the present invention.

Manufacture of patch 2

A composition to be used for patch 2 was prepared using the composition of the following Table 2, using a conventional method for producing a cosmetic composition.

2 Component (content,% by weight) Example water To 100 Betaine 2.0 glycerin 3.0 Cellulose sword 0.1 Bulgarian rose extract (Reference Example 2) 1.0 PEG.PPG-17.6 copolymer 1.0 antiseptic 0.05 to 0.3 Spices 0.005

The above composition was impregnated into a nonwoven fabric prepared as shown in Fig. 1 to prepare a patch 2.

[Reference Example 4] Production of Eye Cream

Eye cream was prepared with the composition shown in Table 3 below.

division ingredient Example Awards Purified water To 100 Disodium iodide 0.05 glycerin 2.0 Butylene glycol 10.0 antiseptic 0.2 Paid Glyceryl stearate 1.0 Polyglyceryl-3 methylglucose distearate 2.5 Dimethicone 3.0 Cyclomethicone 5.0 Additional ingredient Epigallocatechin gallate 0.1 Peptiskin 0.1 Spices 0.001

The production method is as follows.

1) The oil component was heated to 70 캜.

2) The aqueous phase components were dissolved in an agitation mixer while being heated to 75 ° C.

3) The oil component of 1) was mixed with the water component of the above 2), and the cream was prepared using a homogenizer at 8000 rpm for 5 minutes.

4) After the addition, additional components such as an increasing agent were added while stirring, and homogenizer was stirred for 3 minutes at 8000 rpm.

5) Add EGCG, peptiskin, fragrance at 45 ℃ or lower.

6) After thoroughly stirring, it was degassed and then cooled to 30 ° C.

[Test Example 4] Measurement of eye wrinkle improvement effect

The wrinkle improving effect of the eye patch prepared in Reference Example 3 was measured. Measurements were made on 30 Chinese women aged 25 to 39 years, and they were used twice a week for a total of 6 weeks. The degree of wrinkles, elasticity and water content were measured immediately after use, after 3 weeks, and 6 weeks .

The above patch was used in the following manner. After applying the eye cream of Reference Example 4 sufficiently, patch 1 was attached to the under-eye area where intensive care was desired, and lightly tapping on the patch was allowed to take place. I then covered my eyes with patch 2 for 20 minutes. The appearance after application of patch 1 and patch 2 is shown in Fig.

1. Visual evaluation of wrinkles by a specialist

After two eyes of the subjects who visited at first (0w), immediately after use (0w '), 3weeks (3w), and 6weeks (6w) It means that wrinkles decrease toward 1 point.

As shown in FIG. 6, the wrinkle was improved by 2% immediately after use of the product, and the wrinkles were improved by 12% and 19% after 3 weeks and 6 weeks, respectively. have.

2. Evaluation of recovery force after eye wrinkle induction

The skin elasticity was evaluated by evaluating the degree of recovery after deep wrinkling caused by artificially wrinkling the skin of the eye. The eyes were squeezed up and down by 1.5 cm, the tape was stuck, and 5 minutes were awaited, and artificial wrinkles were made in this manner. The tape was then removed and the length of the wrinkles was measured. The total length was set at 3 cm, and the degree of restoring force after use of the product was measured, and the measured length was regarded as skin elasticity. This means that the longer the length, the better the resilience, and 3cm means that the skin has recovered to its initial state.

FIG. 7 shows that the skin length of the eye was significantly increased to 2.85 cm and 2.86 cm after 3 and 6 weeks, respectively, as compared with before the patch use, and artificially induced wrinkles were effectively improved by the increase of the elasticity of the eye by the product can confirm.

3. Self-evaluation of wrinkles of subjects

The degree of wrinkle improvement perceived by the subjects was evaluated using the patch according to the present invention for 6 weeks. The subjects themselves were assessed by using a 5-point scale. The subjects were directly assessed for changes before and after the use of the product and evaluated for cognitive efficacy.

8, the degree of wrinkle improvement was 12% after 3 weeks of use, and 19% after 6 weeks of use, indicating that the patch of the present invention gradually improved wrinkles.

4. Evaluation of skin moisture

The moisture content before and after the use of the product was measured with a Corneometer® (CM825, C + K, Germany) to evaluate how much moisture increased. The values are expressed as relative values measured before and 3 weeks after use, and the unit is AU.

FIG. 9 shows that the moisture content is increased by about 30% immediately after use of the product, and the water supply effect can be immediately confirmed. Also, when the product is not applied after 3 weeks, the water content is increased by 10% And the moisture resistance of the skin of the eyes is increased.

5. Evaluation of the degree of wrinkle improvement through photography

FIG. 10 shows the state of the eye before and after application of the patch of the present invention, and the degree of improvement of the eye wrinkles according to the present invention can be easily evaluated.

The state after use of the product shown in Fig. 10 and after use for 6 weeks can be visually confirmed that the wrinkle reduction, depth, and length are reduced in all of the radial wrinkles under the eye part and the eye part after 6 weeks use.

6. Subject Questionnaire on Efficacy and Quality

As a result of the above experiment, the participants who participated in the experiment were surveyed as follows.

[Question Contents]

Have you experienced improvement in eye elasticity after 3 weeks and 6 weeks?

After 3 weeks, after 6 weeks, have you experienced eye wrinkle improvement?

How satisfied are you with Patch 1 and Patch 2 after three weeks and six weeks?

The subjects perceived improvement in eye elasticity by 77% after 3 weeks and 93% after 6 weeks. They recognized improvement of eye wrinkles by 87% after 3 weeks and 90% after 6 weeks, and 93% And the results are satisfactory.

Claims (9)

delete delete delete Patch 1, a melting film containing acetylglucosamine, adenosine, Pullulan, carob bean gum and carrageenan; And
Patch 2, which is a sheet impregnated with a composition containing a water-soluble extract of a Bulgarian rose bud obtained by homogenizing a Bulgarian rose bud, extracting it with cold water, vortexing, and removing insoluble matter by centrifugation;
Lt; / RTI >
Wherein the acetyl glucosamine is contained in an amount of 0.1 to 5.0 wt% based on the total weight of the patch 1, the adenosine is contained in an amount of 0.01 to 5.0 wt%
Wherein the water-soluble extract of the Bulgarian rose bud is contained in an amount of 0.1 to 10.0% by weight based on the total weight of the composition impregnated in the patch 2,
Patch for improving the moisturizing power of the skin of the eyes. A cosmetic method using the patch of claim 4,
Wherein the patch 1 is attached to the subcutaneous skin and the upper surface of the patch 1 is covered with the patch 2. The cosmetic method for improving the moisturizing power of the skin of the eye area. delete delete delete delete

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