WO2011108907A2 - Composition for relieving pruritus and atopy, containing medicinal herb extracts as active ingredient - Google Patents

Abstract

The present invention relates to a composition for relieving pruritus, containing extracts of Rehmannia glutinosa Libschitz var. purpurea Makino, Acorus gramineus Solander, Phellodendron amurense RUPR., Sophora Flavescens, bamboo leaves, Lithospermum erythrorhizon SIEB., Polygonum aviculare L., Hibiscus syriacus L., Tussilago farfara L., Astrocyindropntia subulata and Aristolochia contorta BUNGE. as an active ingredient, wherein the composition induces the control of skin immunity, thereby relieving pruritus. In addition, the present invention relates to a composition for treating atopy, containing extracts of fresh Rehmannia glutinosa Libschitz var. purpurea Makino, Acorus gramineus Solander, Sophora Flavescens, Lithospermum erythrorhizon SIEB. and Phellodendron amurense RUPR. as an active ingredient. The composition containing the extracts can be variously used as a cosmetic composition, a pharmaceutical composition and a dietary supplement.

Description

Composition for improving pruritus and atopy containing herbal extracts as an active ingredient

The present invention relates to a composition for improving pruritus and atopy containing herbal extracts as an active ingredient.

Due to the rapid changes in the environment and the complexity of society, the occurrence of skin diseases is increasing rapidly. In response to socio-economic development, the improvement of quality of life and the growing interest in skin diseases have increased the demand for new functional cosmetics. I'm making it. In addition, there is no universal treatment for pruritus, so there is a need for a safe and effective product. Until now, the treatment of pruritus mainly used steroids, but because of the limited use of side effects it is necessary to develop a safe pruritus improver.

Itching refers to the condition of the skin that causes discomfort to be scratched. Itching can also be caused by stimuli such as light contact, temperature changes, or stress, or by chemical, physical or electrical stimuli. It is also a symptom associated with skin diseases such as atopic dermatitis, psoriasis, and contact dermatitis or with sensitive skin owners. This unique sensation of pruritus, or itch, or itch, which causes the urge to scratch, is a systemic disease that occurs only on the skin. It shows a pathology similar to eczema As with insect bites, most itching is obvious and its symptoms disappear easily within a short time, but in chronic pruritus, itching can be a cause of mental disorders that go beyond normal life. Itching (pruritus) is known to suffer from about 5% of the world's population, with the most severe between 20 and 30 years of age, and more common in men than women (Shimer et al ., 2000). ). 25% of pruritus have been reported in jaundice and 50% in people undergoing renal dialysis. In addition, pruritus is a chronic disease in which itching is the main symptom, including erythema, edema, and exudate (Hong Chang-eui, 1994).

Because atopic dermatitis and psoriasis with pruritus are accompanied by various inflammatory reactions and immune disorders, natural substances that have anti-inflammatory effects while controlling the immune system may have therapeutic effects, rather than substances that are superior to either immunoregulation or anti-inflammatory. This is considered to be very large. In the case of oriental medicine, research on the vast amount of prescriptions written in many Chinese medicines and studies to prove the effects of herbal medicine are being conducted, but the prevalence of dermatitis patients is gradually increasing, and it has been reported that positive treatment effect can be obtained by taking herbal medicines 2000; Shin Sang-Ho et al ., 2007; Koh et al ., 2006). However, due to the characteristics of the Chinese medicine, the economic burden is relatively large, and due to the peculiar taste and aroma, it may be difficult to take it. As a way of supplementing this part, active use of external preparations can be considered as a priority.

The present invention investigated and reviewed previous studies on external treatment and atopy of pruritus, and summarized the effects of various prescriptions and herbs to develop herbal water using herbal medicines that are expected to improve pruritus and atopy and investigated its function. . In addition, the present invention is a specific plant extract obtained from the natural skin safer than the sulfuric acid, Seokchangpo, baekbaek, red ginseng, bamboo leaf, licorice, defecation, neck root skin, Kwandonghwa, Janggunpi and Mokwa in the conventional skin external composition The present invention relates to a composition containing herbal medicines with the effect of improving pruritus and atopic dermatitis by studying the effect of improving skin immunity of symptoms such as severe itching (pruritus) and inflammation and rash common to skin diseases. The purpose of this study was to verify the effectiveness of the product.

The present invention overcomes the problems of steroids and antihistamines, excellent in human safety and effective in suppressing and alleviating itching, ginseng, Kwanhwa, neck and neck, bark skin, Seokpo, turmeric, bamboo leaf, vinegar, janggunpi, defecation and An object of the present invention is to provide a cosmetic composition for improving pruritus, a pharmaceutical composition, and a health functional food containing the white extract as an active ingredient.

In addition, it is an object to provide a cosmetic composition, a pharmaceutical composition and health functional food as a composition for the treatment of atopic dermatitis containing extracts of raw jihwang, Seokchangpo, Gosam, Japonica, and sulfur white as active ingredients.

In order to achieve the above object in one embodiment provides a cosmetic composition for pruritus improvement containing ginseng, Kwandonghwa, neck and neck, bark skin, Seokchangpo, jihwang, bamboo leaf, jaecho, Jangpipi, pyeonyak and baekbaek extract as an active ingredient.

In one embodiment provides a pharmaceutical composition for improving pruritus, containing ginseng, Kwandonghwa, neck, neck roots, Seokchangpo, Chihwang, bamboo leaf, licorice, Janggunpi, shrunk and baekbaek extract as an active ingredient.

In one embodiment provides a functional food for improving pruritus containing ginseng, Kwandonghwa, neck and neck, bark skin, Seokchangpo, Chihwang, bamboo leaf, vinegar, Janggunpi, shrunk and baekbaek extract as an active ingredient.

In one embodiment there is provided a cosmetic composition comprising a composition containing raw jihwang, Seokchangpo, red ginseng, licorice, yellow white extract as an active ingredient.

In one embodiment there is provided a pharmaceutical composition comprising a composition containing raw jihwang, Seokchang pho, Gosam, Jacho, yellow white extract as an active ingredient.

In one embodiment provides a dietary supplement comprising a composition containing raw jihwang, Seokchangpo, red ginseng, licorice, baekbaek extract as an active ingredient.

The composition containing the herbal extract according to the present invention as an active ingredient is a specific plant extract mixture obtained from the natural plants safe for human body, such as jihwang, Seokchangpo, baekbaek, ginseng, bamboo leaf, vinegar, pinch, mokeunpi, kantohwa, janggunpi and mokwa It is an herbal cosmetic formulated with medicinal herb and has an effective effect on improving the skin immune condition, skin moisturizing and wrinkle improvement of symptoms such as itching (pruritus) and inflammation and rash.

In addition, the composition for the treatment of atopic dermatitis containing raw jihwang, Seokchangpo, red ginseng, vinegar, yellow white extract as an active ingredient can be used in a variety of cosmetic compositions, pharmaceutical compositions and health functional food.

1 is a graph showing the ability to inhibit COX-2 activity according to pruritus improved herbal lotion concentration.

Figure 2 is a graph showing the ability to inhibit LOX-5 enzyme activity according to pruritus improved herbal lotion concentration.

Figure 3 is a graph showing the flavonoid (flavonoid) content according to the pruritus improved herbal lotion concentration.

Figure 4 is a graph showing the convergence effect of pruritus improved herbal lotion concentration.

5 is a graph showing the elastase inhibitory activity of pruritus improved herbal lotion.

Figure 6 is a graph showing the frequency of pruritus over time of the LTB ₄ induced itch model.

7 is a graph showing the total number of pruritus of the LTB ₄ induced itch model.

8 is a graph showing the effect on the IL-6 concentration of the skin culture medium of the LTB ₄ induced itch model.

9 is a graph showing the effect on the plasma INF-γ concentration of the LTB ₄ induced itch model.

10 is a graph showing the frequency of pruritus over time of the compound48 / 80 induced itch model.

11 is a graph showing the total number of cultivation of the compound 48/80 induced itch model.

12 is a graph showing the plasma IL-4 concentration of the compound48 / 80 induced itch model.

13 is a graph showing the effect on the plasma INF-γ concentration of the compound48 / 80 induced itch model.

Figure 14 is a graph showing the scratch frequency with or without the prescription of herbal creams (including atopy solution) of the atopy-induced animal model (mean ± SEM: P <0.05).

Figure 15 is a graph showing the scratch strength with or without the prescription of herbal creams (including atopy solution) of the atopic dermal animal model (mean ± SEM: a> b).

FIG. 16 is a graph of the total IgE levels measured by ELISA in plasma (mean ± SEM: a> b).

17 is a graph showing the IL-6 production amount of the skin cell culture fluid.

Hereinafter, the present invention will be described in detail by the following examples. However, the following examples are merely to illustrate the invention, but the content of the present invention is not limited by the following examples.

EXAMPLE

Example 1 Preparation of Pruritus Improvement Lotion

1-1. Sample Preparation

A total of 11 kinds of herbal materials related to pruritus and skin health were selected by collecting data such as ancient books and literatures (Table 1). The medicinal herbs used in the experiments were purchased in a dry state from the herbal medicine Love and Namdo Herbal Agricultural Cooperative Corporation, and stored and frozen. The organic solvents used in this study were grade 1 and special grade, and all reagents used for functional verification were those of biochemical grade or higher.

Table 1

Herbal name	Scientific name
High hemp	Sophora flanescens
Kanto	Tussilago farfara L.
Neck and	Chaenomeles sinensis Koehne .
Neck Root	Gossampinus malabarica
Seokchangpo	Acori Graminei Rhizoma ( Acorus gramineus Sol.ex Aiton)
Yellow	Rehmanniae Radix (Rehmannia glutinosa (Gaertner) Liboschitz)
Bamboo leaves	Bamboo leaf
Candle	Lithospermum erythrirhizon Sieb. et Zucc.
General Blood	Rhus chinensis MILL ( Rhus javanica L.)
Single axis	Polygonum aviculare L.
Sulfur bag	Phellodendri Cortex

            

1-2. Extraction of samples

The herbs selected through the literature were extracted repeatedly at room temperature with distilled water and 70% alcohol. That is, in the case of bamboo leaf and turmeric, distilled water was added 100 times per gram of the weight of herbs, and extracted twice by dipping at room temperature for 2 days.The remaining herbs except bamboo leaf and turmeric were 4 times 70% alcohol per gram of the weight of each medicine. The extract was repeatedly extracted three times by immersion at room temperature for 2 days. Each extract was filtered under reduced pressure using a pore size 5㎛ paper filter paper (Advantec, No. 5), and the 70% alcohol extract was concentrated with a reduced pressure concentrator (Eyela, Japan) and dried thoroughly. The extract was freeze-dried and stored frozen.

1-3. Preparation of herbal lotion

Herbal cosmetics prepared for the present invention is 11 kinds of selected herbal medicines having the effect of improving pruritus on the basis of the flexible cosmetics in the same manner as described above and then mixed these extracts by using a pore size 0.2㎛ filter paper Filtration to prepare herbal cosmetics.

Example 2 Preparation of Atopic Improvement Cream

2-1. Sample Preparation

The medicinal herbs used in the experiments were purchased in a dry state from the herbal medicine Love and Namdo Herbal Agricultural Cooperative Corporation (Table 2). The organic solvents used in this study were grade 1 and special grade, and all reagents used for functional verification were those of biochemical grade or higher.

TABLE 2

Herbal name	Scientific name
Living	Rehmanniae Radix (Rehmannia glutinosa (Gaertner) Liboschitz)
Seokchangpo	Acori Graminei Rhizoma ( Acorus gramineus Sol.ex Aiton)
High hemp	Sophora flanescens
Candle	Lithospermum erythrirhizon Sieb. et Zucc.
Sulfur bag	Phellodendri Cortex

2-2. Extraction of samples

Each medicine was extracted repeatedly at room temperature with 70% alcohol. In other words, each of herbs such as raw jihwang, Seokchangpo, red ginseng, vinegar and yellow baek was repeatedly extracted three times by adding three times 70% alcohol solution per gram weight and soaking at room temperature for 3 days. Extracts of each medicine were used under reduced pressure filtration using a pore size 5㎛ paper filter paper (Advantec, No. 5).

2-3. Preparation of Herbal Cream

For later experiments, the atopy solution was prepared by mixing the ratios of sulfuric acid: yellowish white: Seokchangpo: red ginseng: licorice in a ratio of 3: 2: 1: 1: 1. In addition, the herbal extract was prepared to be 10% of the total capacity of the mixed extract for animal experiments.

Example 3 Functional Confirmation of Pruritus Improvement Lotion

3-1. Pruritus effect

The pruritic effect is related to the anti-inflammatory effect, and the anti-inflammatory effect can be explained in part by the excellent antioxidant effect of herbal toiletries. In the present invention, the effects of inhibiting COX-2 activity and inhibiting the activity of 5-lipoxygenase to determine the anti-inflammatory mechanism of herbal cosmetics other than the antioxidant action was measured.

(1) COX-2 inhibitory ability

In order to effectively manage skin diseases with inflammation and some immune system abnormalities, it is essential to use a therapeutic agent that exhibits high anti-inflammatory and immunomodulatory effects and minimal side effects. Cyclooxygenase (CYCLO-OXYGENASE-2; COX-2) production inhibitory activity, moisturizing effect experiment was used as a method for searching for a substance having these characteristics. Herbal cosmetics are expected to be anti-inflammatory effect by showing the ability to inhibit COX-2 activity in a concentration-dependent manner (Fig. 1).

(2) LOX-5 inhibitory ability

IL-8 is known to cause neutrophil increase by activating the lipid mediator 5-lipoxygenase to produce leukotriene B ₄ (LTB ₄ ) (Frank et al. , 2005). Therefore, it was confirmed that the ability to inhibit LTB ₄ synthesis, which is an itch-inducing substance, by LOX-5 activity inhibitory ability, was confirmed to be inhibited in a concentration dependent manner (FIG.

3-2. Moisturizing effect

People with pruritus usually have dry skin, and itching is the most important symptom and itching leads to rashes and causes itching again. The skin is chronically dry, has a strong pruritus and recurs repeatedly, and dermatitis is easily caused by various stimuli. If not properly managed, it progresses to subacute lesions of erythematous rash with exudation and thickened, electoral, chronic lesions such as leather (Anseong-gu, 2007).

(1) Flavonoid Content Determination

Flavonoid content positively correlated with anti-inflammatory response, astringent effect and antioxidant capacity was measured in oriental cosmetic water. The intracellular anti-inflammatory mechanism of flavonoids is known to be inhibited by cyclo-oxygenase (COX) and lipoxygenase (LOX), which are eicosanoid synthase. (Ferrandiz et al., 1990; Middleton et al., 1992).

Flavonoid content of herbal cosmetics contains 60ug / ml (quercentin equivalent) (Fig. 3). Flavonoids are considered to be an important part of skin health function by having positive relationship with COX-2 inhibitory effect, convergent moisturizing effect and antioxidant power.

(2) convergence effect

In the experiment of astringent effect, the degree of astringent effect is judged according to the degree of binding of hemoglobin protein to the extract. The astringent experiment using herbal cosmetics can be measured according to the method of Lee et al., Using a blood protein (Hemoglobin from Bovine, Sigma Co., USA) that is similar to skin protein and can be easily obtained, After mixing each herbal cosmetics and hemoglobin solution in a 1: 1 shake and centrifugation, the absorbance was measured at 576 nm to measure the convergence effect. The convergence effect measured by hemoglobin precipitation method of herbal cosmetics was shown in a concentration-dependent manner (Fig. 4).

3-3.Elastase Inhibition Activity Evaluation

To investigate the different functionalities of herbal cosmetics, 25 ° C was added by adding 1 unit elastase and 0.8 mM N- suc- (ala) ₃ -p-nitroanilide to 0.2M Tris-HCl buffer (pH 8.0). After reacting for 30 minutes at, the absorbance was measured at 410 nm to quantify the amount of p-nitroanilide generated from the substrate. The comparison group used ursolic acid.

Herbal cosmetics showed an elastase inhibitory activity of 52% at a concentration of 1 mg / ㎖, showing a similar activity to the uronic acid showing 63.8% of activity (FIG. 5).

Example 4 Pruritosis Inhibitory Effect in Animal Models

4-1. LTB ₄ Induced Itching Model

(1) preparation of materials

Male ICR mice, 5-6 weeks old, were bred under the conditions of illumination at 25 ± 2 ° C and 08: 00-20: 00 hours. Water and feed were free to eat. After epilating the back of the mouse clean, left for 24 hours to heal the fine wounds of the skin. And 100 μL of leukotriene B4 (LTB ₄ ) 0.03nmole / site was injected into the back area, and the negative control group ONO-4057 (5- [2- (2-carboxyethyl) -3- (6 (p-methoxyphenyl) -5E) -hexenyl) oxypheoxyl] valeric acid) was injected with 100 μL of 0.03 nmole / site. 100 μL of distilled water was treated as a positive control group of external water, and 100 μL of herbal cosmetic water was treated as an experimental group.

(2) Effect on the number of cultivation with time

In the LTB ₄ induced itch model, the frequency of pruritus over time was significantly reduced in the experimental group after 40 minutes of itch induction (Fig. 6).

(3) Effect of oriental cosmetics on the number of cultivation

In the LTB ₄ induced itch model, the total number of cultivation was significantly decreased in the negative control group compared to the positive control group, and the experimental group was significantly reduced than the negative control group (NC), which significantly reduced the total cultivation frequency ( 7).

(4) Effect of Oriental Cosmetics on Immunological Factors

In the LTB ₄ induced itch model, IL-6 concentration of the skin culture was highest in the positive control group (PC) and the experimental group was significantly reduced to the negative control (NC) level (Fig. 8). In addition, plasma INF-γ concentration was significantly increased in the experimental group (Fig. 9). In general, IFN-γ is considered to be a Th1-related cytokine that is reduced in inflammation and itching, so oriental cosmetics may be interpreted to have an effect on itching.

4-2. Histamine-induced itching model

Induction of histamine release using Compound48 / 80 as a degranulation agent for mast cells, followed by itch-curing animal model that stimulates histamine-reacted C-fiber (itch-specific fiber) to induce itching , Pruritus (severity of itching), immunological factors (INF-γ involved in cellular immunity: Th2-related cytokine involved in humoral immunity; IL-4) were measured, respectively.

(1) preparation of materials

11 weeks of age, BALB / c (20-26 g) mice were depilated cleanly, and left for 24 hours to heal fine wounds on the skin. After injection of 100 μL of compound 48/80 solution (μg / site) into the back, 100 μL of the antagonist Diphenhydramine hydrochloeide (St. Louis, MO, USA) was orally administered as a negative control. In the positive treatment group of external water, 100 μL of distilled water was treated, and the experimental group was treated with 100 μL of herbal cosmetic water.

(2) Effect of Oriental Cosmetics on Total Frequency

As a result of investigating the frequency of cultivation over time in the compound48 / 80-induced itch model, the cultivation frequency of oriental cosmetic treatment group decreased significantly with the frequency of negative control (NC) level ( p <0.05) (FIG. 10).

(3) Effect of oriental cosmetics on the total number of cultivation

In the compound 48/80 induced itch model, the total number of cultivation was examined, and the oriental cosmetic treatment group decreased to the negative control (NC) level ( p <0.05) (FIG. 11).

(4) Effect of Oriental Cosmetics on Immunological Factors

In the compound48 / 80-induced itch model, the effects of Th2-related cytokine on the IL-4 concentration of plasma were investigated, and the concentration of negative control group (NC) was decreased compared to the positive control group (PC) and decreased in the herbal treatment group. (FIG. 12).

In addition, as a result of examining the effect on plasma INF-γ concentration in the compound48 / 80 induced itch model, the concentration of the negative control (NC) was significantly reduced compared to the positive control (PC) (Fig. 13).

Example 5 Atopic Inhibitory Effects in Animal Models

5-1. Preparation of the ingredients

After removing the back of the 11-week-old NC / Nga mice, they were left for 24 hours to heal fine wounds on the skin. Then 200 μL of 1% DNCB solution (acetone: olive oil = 3: 1) was applied to the back area, and after 4 days, 150 μL of DNCB solution was applied to the back area 2-3 times a week (from 12 to 16 weeks). . After 4 weeks of treatment, dermatitis was sufficiently induced, and all of the back skin was peeled off, and new dermatitis was formed on the site, so that the scratching action was intensified. DNCB treatment was stopped, and sensory evaluation was performed. 11-week old BALB / c mice were used as a normal group.

The dermatitis-induced mice were divided into two control groups (without atopy solution) and experimental group (20% atopy solution), and the two creams were applied for 5 weeks (from 12 to 17 weeks). After the sensory evaluation before and after drug treatment, blood was taken, and skin and lymph from the back and left ear were excised and stored in 10% formalin solution.

5-2. Histological evaluation

The skin tissue was examined to see how the herbal cream containing atopy solution affected the skin tissue. Skin tissues were observed mainly on the overall condition of the skin, infiltration and degranulation of mast cells, and eosinophil infiltration. One of the characteristics of atopic dermatitis is that immune cells such as T cells, mast cells and eosinophils are infiltrated into the skin, and the epidermis and dermis become thick and bleeding occurs. Therefore, tissue staining was performed to confirm whether the atopy solution alleviated the symptoms of atopic dermatitis by preventing the infiltration of immune cells into the skin tissue.

Skin tissue immobilized in 10% formalin was embedded in paraffin, cut into 4um thickness, and stained with hematoxylin-eosin. In order to examine the infiltration of inflammatory cells, mast cells were stained by toluidine blue staining, and tissues were stained using congo red staining to confirm eosinophils. The magnification of was observed.

As a result, in the hematoxylin-eosin staining, the regular arrangement of tissue cells such as epithelial cells of the control group was destroyed, but the regular arrangement of tissue cells such as epithelial cells of the experimental group was observed. Toluidine blue staining, and Congo red staining, which stained mast cells, inhibited the degranulation of mast cells and their cells infiltrating skin tissue by atopic induction.

5-3. Pruritus Behavioral Effects

In the case of atopic dermatitis, which is considered an immune disease, many studies have reported an increase in serum IgE levels. Atopic patients also have severe itching (pruritus). Analysis and comparison results to confirm the degree of pruritus, which is one of the general clinical symptoms of atopic dermatitis, are shown in Table 3, FIGS. 14 and 15.

TABLE 3

	Total number of scratches 1)	Scratch strength figures 2)
Control	169.89 ± 20.93	95.71 ± 10.63
Experimental group	106.53 ± 16.65 *	60.30 ± 16.44 *
Average scratching of mice for 5 weeks. (/ 60 min) n = 8 Scratching behaviors of mice in all groups were taken with a video camera for 60 minutes. 1) This result calculated one period at a time. Was measured by the standard shown previously.

As the atopy is induced in the present invention, the number of scratching was increased, and when comparing the results of Weeks 2 and 3, the number and frequency of scratching were significantly lower in the experimental group of the present invention than in the control group.

On the other hand, Takahashi's study showed that scratching caused by pruritus increased in NC / Nga mice, an animal model of atopic dermatitis, and that the number of scratches in skin lesions in NC mice increased more than in skin-free lesions in NC mice. Reported.

5-4. Effect of IgE in Plasma

IgE is an antibody that plays an important role in promoting atopic dermatitis by activating mast cells and is generally increased in atopic patients.

At 12, 15, 18, and 21 weeks of application, approximately 100 μL of blood was collected from the mice's eyes using capillaries, followed by centrifugation at 6,500 rpm for 20 minutes, and 30 μL of serum was isolated. . Serum was taken and stored frozen at −70 ° C. until used for experiments. Serum IgE was measured by ELISA kit (K & D system). Antibodies were diluted in coating buffer and coated in microwells and then placed at 4 ° C. overnight. Each well was washed three times with a wash buffer solution and then 100 μL of serum (100-fold dilution) was dispensed. This was allowed to stand at room temperature for 1 hour, washed twice with a wash buffer solution, and then treated with 100 μL of antibody avidin-HRP conjugated. The TMB substrate was dispensed by 100 μL and left in the dark for 30 minutes, and then treated with 50 μL of stop solution, and the absorbance was measured at 450 nm of ELISA reader. After 21-week-old NC / Nga mice were anesthetized with ketamine hydrochloride, blood was drawn by cardiac puncture to separate serum and enzymes were analyzed for IL-4, 5, INF-γ, IgM, and IgG1 in serum. Quantification was performed with an immunoassay kit (ELISA kit).

Plasma IgE was significantly decreased by 20% in 117.25 ± 6.47ng / ml in normal group, 133.92 ± 7.59ng / ml in control group and 106.58 ± 8.11ng / ml in experimental group (P <0.05). IgE is an antibody that plays an important role in promoting atopic dermatitis by activating mast cells, and is generally increased in atopic patients. In the present invention, the total IgE content in plasma was increased in the atopic dermatitis control group compared to the normal group. However, the experimental group significantly decreased to the level of the normal group. It was found that the experimental group reduced the IgE content in the plasma. In other words, it is 20% lower than that of the control group, and the above results suggest that the experimental group is effective in lowering the level of IgE, an immune factor, but is not direct but may help to alleviate the symptoms of atopy (Fig. 16). ).

5-5. Effect of Skin Cell Culture Solution on IL-6

IL-6 is a cytokine produced from several types of epithelial cells, such as activated macrophages, endothelial cells, and fibroblasts, which act on both innate and adaptive immunity, is involved in the growth of B lymphocytes, and mediates an overactive immune response.

After epilating the back of NC / Nga mice, 1 g of skin tissue was removed, washed with DMEM culture medium, chopped into fine pieces, and replaced with 10% FBS-DMEM. After separating the culture supernatant for 7 days, the IL-6 secretion of the culture was measured by an enzyme immunoassay kit.

In the invention of IL-6 production of skin cell culture fluid, the DNCB-induced atopic dermatitis was increased in the control group compared with the normal group. In the experimental group, the IL-6 production was decreased compared to the control group, showing the inhibitory effect of IL-6 production. (FIG. 17).

While the invention has been described with reference to exemplary embodiments so far, those skilled in the art will appreciate that various changes can be made therein and equivalents may be substituted for elements thereof without departing from the scope of the invention. You will know. In addition, many modifications may be made to adapt a particular situation and material to the teachings of the invention without departing from the essential scope thereof. Therefore, it is intended that the present invention not be limited to the particular embodiments disclosed as the best mode contemplated for carrying out the invention, but that the invention will include all embodiments falling within the scope of the appended claims.

Claims (6)

1. A cosmetic composition for improving pruritus, which contains red ginseng, Kwandonghwa, neck and neck, neck root, Seokchangpo, Chihwang, bamboo leaf, Korean herbaceous, Janggunpi, Prunus, and yellowish white extract as active ingredients.
2. Pharmaceutical composition for the prevention and treatment of pruritus, which contains red ginseng, Kwandonghwa, neck and neck, neck root, Seokchangpo, Chihwang, Bamboo Leaf, Licorice, Janggunpi, Skeletal and Yellowish White Extract as active ingredients.
3. Health functional food for improving pruritus, which contains red ginseng, Kwandonghwa, neck, neck root, Seokchangpo, Chihwang, Bamboo Leaf, Licorice, Janggunpi, Skeletal and Yellow White Extract as active ingredients.
4. A composition for improving atopic dermatitis containing raw jihwang, Seokchangpo, red ginseng, licorice, and yellowish white extract as active ingredients.
5. A pharmaceutical composition for the treatment of atopic dermatitis, containing raw jihwang, Seokchangpo, Gosam, Jacho and yellow white extract as active ingredients.
6. Health functional food for improving atopic dermatitis containing raw kojihwang, Seokchangpo, red ginseng, vinegar and yellow white extract as active ingredients.

Images