KR20120118222A - Cosmetic composition for deoderizing odl men smell by using orental natural materials - Google Patents

Abstract

PURPOSE: A natural oriental medicine cosmetic composition for removing old person's body ordor is provided to enhance anti-oxidant activity and anti-biotic and anti-septic effects. CONSTITUTION: A natural oriental medicine cosmetic composition for removing old person's body ordor comprises Phellinus linteus extracts, Sophora flavescens Solander ex Aiton extracts, Scutellaria baicalensis GEORGE extracts, Zanthoxylum Piperitum extracts, Pulsatilla Koreana extracts and Usnea Barbata(Lichen) extracts. 0.5-1.5 wt% of the phellinus linteus extract is included for the total weight of the cosmetic composition based on the dry weight standard. 0.2-1 wt% of Sophora flavescenes extracts, Scutellariae radix extracts, zanthoxylum piperitum extracts and Usnea barba extracts are included based on the dry weight standard. Each extract is obtained by supercritical fluid extraction, hydrothermal extraction or ethanol extraction method. The cosmetic composition is phase of liquid, cream, paste or solid.

Description

Herbal cosmetic composition for deodorizing the elderly {Cosmetic composition for deoderizing odl men smell by using orental natural materials}

The present invention relates to a natural herbal cosmetic composition for removing the elderly odor, and more particularly, it contains a natural herbal ingredient excellent in antioxidant activity and antibacterial / antiseptic effect, which can remove the odor generated in the elderly, that is, the elderly odor. It relates to a natural herbal cosmetic composition.

Recently, anti-aging cosmetics for the purpose of preventing aging are expanding the area from recovery to recovery through more advanced technology. However, in Korea, it is overflowing with functional cosmetics for delaying aging and wrinkle improvement, but it is hard to find a product exclusively for 'old people'.

Beauty and personal health care products for the elderly and those who suffer from the peculiar smell of aging caused by aging are constantly being demanded. Due to such high demand, the smell of the elderly is known as Many products are released one after another. In the US, the age-friendly cosmetics market is $ 3.64 billion (2007), and in Japan, there are more than 300 kinds of aged-friendly cosmetic products (2007). As such, advanced countries such as North America and Japan are actively developing aged-friendly cosmetics, while in Korea, the development of such aged-friendly cosmetics is low. In addition, the elderly deodorant products are small enough to be seen in the domestic industry. Domestic cosmetics companies are delaying the development of age-friendly cosmetics due to marketability.

Applicants' survey data show that 88.5% of households between the ages of 55 and 75 use cosmetics and prefer wrinkle-reducing products and body deodorant products. However, the deodorant products on the market are not focused on the elderly, but are aimed at the 2,30s, the main customer, and are not tested for safety for the elderly. Mostly. In addition, products that are listed as exclusive products for the elderly also cause another problem due to many side effects by using aromatic compounds to mask the strong fragrance component of the elderly body odor.

In particular, in old age, cis-9hexadecenoic acid (C ₁₆ H ₃₀ O ₂ ), a fatty acid contained in sebum, is oxidized and decomposed to produce a characteristic elderly smell. It is a substance called nonenaldehyde aldehyde (C ₉ H ₁₆ O), which is produced mainly in the elderly, but occurs in patients suffering from chronic diseases, especially in patients with high mobility. In other words, the metabolism is not smooth and more waste is left on the skin, and more nonenaldehyde, which is produced by the breakdown of unsaturated fatty acids in these sebaceous glands, is made and the smell is released. Therefore, antioxidation of cis-9hexadecenoic acid, which is a reactant discharged through sebaceous glands in the body, is expected to suppress the elderly odor.

Accordingly, the present invention contains antioxidants derived from natural products that inhibit the oxidation of waste products such as cis-9hexadecenoic acid, which occur in the skin of skin, especially the elderly, and also inhibits the action of bacteria caused by lipid peroxide of the skin. An object of the present invention is to provide a natural herbal cosmetic composition for removing elderly odors containing antibacterial / preservatives derived from natural products.

According to the present invention, there is provided a natural herbal cosmetic composition for removing the elderly odor, characterized in that it comprises a situation mushroom extract, a ginseng extract, golden extract, sancho extract, Baidu extract and moss extract.

The cosmetic composition of the present invention can be usefully used as a cosmetic composition for removing the odor, that is, the elderly odor generated in the elderly due to the excellent antioxidant activity and antibacterial / antiseptic effect.

In addition, since the cosmetic composition of the present invention uses a natural herbal material, the replacement effect of the existing synthetic preservatives can be expected sufficiently.

In the present invention, the situation mushroom extract is used as an antioxidant derived from natural products that inhibits waste products such as cis-9hexadecenoic acid, which occurs in the skin of the elderly, in particular, the skin of the bacteria caused by lipid peroxide of the skin. When ginseng extract, golden extract, sancho extract, baek hong extract and moss extract were used as antibacterial / preservatives derived from natural products that inhibit the action, the present invention was completed by expressing that the elderly odor, aka odor, was significantly removed.

Hereinafter, the present invention will be described in more detail.

Situation mushroom used in the present invention is the name Pelinus linteus ( Phellinus linteus ), also known as the moss-mushroom, in the synonym is written on the liquid juice pieces under the name Sangmokyi (桑 木耳). The shade is 6-12cm in diameter and 2-10cm thick, and has various shapes such as semicircle shape, flat shape, round mountain shape, and horseshoe shape. Dark brown hairs on the surface are short and dense, and then disappear and cut off as they grow. There is a blackish brown ring groove and the back is split horizontally and vertically. Edge is bright yellow, bottom is yellowish brown, and flesh is yellowish brown. No sack, spores pale yellowish brown. It is a perennial tree that grows on mulberry trees and is a wood fungus. In the early days, mud lumps seemed to be agglomerate, and after they are grown, they are sometimes called snow solstice because the tongue is placed on a stump. It is known for its anti-cancer effect, and is a valuable herb and is grown in large quantities in Korea. For medicinal use, the moon appears clear as yellow or light yellow and has no taste and aroma. The taste is mild and it is good to eat. It is native to Korea, Japan, Australia and North America.

The ginseng used in the present invention belongs to the legumes as Sophora flavescens Solander ex Aiton , and it is a perennial plant that grows in the mountains and fields of Korea and is 80 ~ 120 cm tall. There are short hairs of yellow. The roots are hypertrophy, very bitter, the stems stand straight, the leaves are regenerated, the pinnate leafs, the small leaves are 10 pairs, 2-4cm, the edges are flat, the chin leaves are thread-shaped, 5-8cm long, petiole 15 ~ 25cm long, light yellow, 15 ~ 18mm long, many flowers forming a gunshot inflorescence, attached to the end of stem, and fruit is in the shape of a beads by inserting it narrowly between stalk, round barrel, seed and seed. June-August, fruiting season is September-October, root is used for medicinal purposes.

The gold used in the present invention is the scientific name Scutellaria baicalensis GEORGE, a perennial herb belonging to the Lamiaceae family, native to the East Asian continent, native to the mountains of Korea, often cultivated in fields, and height 60 It's about a centimeter. Hairs all over, stems are dense and square, with many branches. Leaves are opposite, few petioles, 4.5 cm long, 8 mm wide, without sawtooth at edges. Flowers attach on inflorescences, purple, end of main stem and branch. Calyx is bell-shaped, closed after flowering, corolla is lip-shaped, corolla is about 2.5 centimeters long. Fruits are inside calyx, rounded. Young sprouts are edible and the roots are used for diuretics, anti-inflammatory antipyretic, and hemorrhagic inflammation.

Sancho used in the present invention is scientific name Zanthoxylum Piperitum ( Zanthoxylum Piperitum ), has a peculiar smell, taste is spicy, warm properties and poisonous. Appearance is a capsule consisting of 2-3 divisions, each branch is spherical and divided into 2 segments. The outer surface of the fruit peel is dark yellowish red or dark red with many concave small dots due to the loss, and the inner side is light yellowish white. In addition, recently, essential oils extracted from the seeds of the Japanese herb have been reported to have local anesthesia and analgesic action, and antibacterial action has an inhibitory effect on Escherichia coli, erythrocytes, cocci, diphtheria bacteria, Staphylococcus aureus, dermatophytes and the like. Sancho is a type of visceral stimulant and is also used as a dry suit. Spicy acid has a strong local anesthetic effect and has an insecticidal effect, eliminating bugs and fish poisons. It is said that it is effective for skin diseases for medical use, and it is known that when lacquer rises, it is better to wash the skin by putting sancho leaves and bathe in bath water.

Baekduhong used in the present invention is a scientific name Pulsatilla Koreana ( Pulsatilla Koreana ), the root has anemonin and tannin. Fresh roots are in the form of protoanemonine, and in the air, two molecules polymerize into anemoneins. In addition, four saponins containing hederagenin as a non-sugar fraction were isolated. Root decoction has the effect of lowering blood pressure, killing amoeba protozoa, and outpost decoction is known to enhance peripheral vasodilation and heart contraction.

The moss used in the present invention is a scientific name Usnea Barbata (Lichen), which is the first plant that has risen from the water to the ground, and lives on the ground, but is gathered in a moist and shady place like a fungus. It has chloroplasts, photosynthesis and breeds as seeds. Water and nutrients are absorbed throughout the body. Its color is green and its shape is gathered in groups of pine needles and umbrella shapes. Research on extracting anti-aging substances from polarized lichens and using them in cosmetics is underway.

Each of the extracts contained in the cosmetic composition of the present invention can be obtained by a conventional extraction method generally known. For example, it can be obtained by at least one extraction method of supercritical extraction, hot water extraction and ethanol extraction. Each ingredient can be used after purchase in a medicinal herb after it is grown or harvested and dried in the sun or shade. In one embodiment, each material was washed, powdered, and cooled for 5 days in an extract solvent of about 10 times, respectively, and then the extract powder was dried to obtain an extract of each material by filtration, concentration, and lyophilization. . Extraction solvents used here include purified water, methanol, ethanol, propanol, butanol, glycerol, propylene glycol, 1,3-butylene glycol, methyl acetate, ethyl acetate, benzene, hexane, diethyl ether and dichloromethane. One or more selected solvents may be mixed and used.

It is possible to prepare a cosmetic composition through a conventional cosmetic preparation method generally known using the situation mushroom extract, goose extract, golden extract, sancho extract, Baekduhong extract and moss extract obtained as described above. For example, a cosmetic such as body lotion or body cleanser may be prepared by mixing cosmetic ingredients generally used such as glycerin, purified water, and glycol distearate with each extract obtained as described above.

The situation mushroom extract obtained as described above was confirmed to exhibit a strong antioxidant activity, and the extract of red ginseng, golden, Sancho, Baekduhong and moss was confirmed to have excellent antibacterial and antiseptic effect (see Examples below). In addition, when the cosmetics such as body cleanser and body lotion containing the situation mushroom extract obtained as described above was excellent in the antioxidant effect and antibacterial / preservative effect, no side effects do not occur situation mushroom, red ginseng, golden, Sancho , Baekduhong and moss extract was found to be used as a safe and effective cosmetic composition.

The amount of the situation mushroom extract is not particularly limited thereto, but may be contained in an amount of 0.5 to 1.5% by weight based on the total weight of the composition on a dry weight basis. This is because the antioxidant effect can not be expected at less than 0.5% by weight, there is no noticeable increase in effect with the increase in content over 1.5% by weight and there is a difficulty in the safety or formulation formulation.

Each of the ginseng, golden, sancho, Baekduhong and moss extract is not particularly limited thereto, but may be contained in an amount of 0.2-1% by weight based on the total weight of the composition on a dry weight basis. This is because less than 0.2% by weight antibacterial and antiseptic effect can not be expected, if it exceeds 1% by weight there is no apparent increase in effect due to the increase in content, there is a difficulty in the safety or formulation formulation.

The cosmetic composition according to the present invention is preferably synergistic effect on the main effect within the range that does not impair the main effect of the present invention, in addition to the above situation mushroom extract, ginseng extract, golden extract, sancho extract, Baidu extract and moss extract It may also contain other cosmetic additive components that can give.

Natural herbal cosmetic composition for removing the elderly odor according to the present invention can be prepared in a variety of cosmetics, including liquid, paste, solid, cream, etc., there is no particular limitation on the formulation as long as the function is not impaired.

Hereinafter, the present invention will be described in more detail by way of examples.

<Examples>

1. Materials and Methods

A. Antioxidant Test on Situation Mushroom Extract

1) experimental material

The situation mushrooms used in this experiment were supplied from Ryu Chung-Hyun Medicinal Mushroom Farm and used as samples after removing moisture using Freeze-dry (Samwon Freezing engineering Co., SFDTS 10K)). In addition, each extraction was used to separate the extract of high concentration and high purity using a supercritical extraction method and hot water / ethanol extraction at the same time. Sampling conditions are as follows.

Sample number Temperature (℃) Pressure (bar) extract Residue One 13 40 150 2 14 50 3 15 40 200 4 16 50 5 17 40 250 6 18 50 7 19 60 8 20 40 300 9 21 50 10 22 60 11 Ethanol extraction (after filtration concentration, normalized to 100 ml) 12 Water extraction (after filtration concentration, apply to 100 ml)

※ Supercritical extraction of samples [1 ~ 10, 13 ~ 22]

2) antioxidant test

① Determination of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity

The antioxidant activity of each extract was measured by slightly modifying the DPPH radical-scavenging activity assay presented by Abe et al. (Biosci. Biotech. Bioch., 64: 306-333, 2000). That is, 100 μL of the sample solution diluted in 900 μL of DPPH (Sigma Chemical Co., St. Louis, MO, USA) ethanol solution (100 μM) was added, mixed lightly with a vortex mixer, and then reacted in a cow for 20 minutes. Absorbance was measured at 517 nm.

② 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS +) radical-measurement of scavenging activity

Antioxidant activity of each extract was determined by ABTS + radical-scavenging. That is, the ABTS + radical solution is 2.5 mM dissolved in 1.0 mM 2,2'-azobis (2-amidino-propane) dihydrochloride (Sigma Chemical Co., USA) in phosphate buffered saline (PBS).

Mixing with ABTS and reacted for 30 minutes in a 70 ℃ thermostat was used for the experiment. PBS used a solvent containing 150 mM NaCl as 100 mM potassium phosphate buffer (pH 7.4).

The concentration of blue-green ABTS + radicals was adjusted to 0.7 ± 0.02 (mean ± SD) at 734 nm using a spectrophotometer. 10 μL of each diluted sample solution was added to the 980 μL ABTS radical solution, reacted at 37 ° C. for 10 minutes, and the absorbance was measured at 734 nm to investigate the ABTS + radical-scavenging activity of the sample.

③ Measurement of SOD (Superoxide radical scavenging) activity

Superoxide radical scavenging activity of each extract was characterized by ultra weak chemiluminescence analyzer (UWLA, BPCL-2-KIC, Institue of Biophysics, Academy) presented by Jeong et al. (Radiat. Phys. Chem., 78: 575-577, 2009). of Science, Beijing, China). That is, 2,1 mL of reaction solution (1.0 mL of 2.0 mM lucigenin,

1.0 mL of PBS (pH 7.4), 0.05 mL of 1.0 M arginine, and 0.05 mL of 1.4 μM methylglyoxal were added to a quartz round sea cuvette to induce radical generation for 13 minutes, followed by the addition of the diluted sample solution to ultra weak chemiluminescence

Measured by an analyzer.

B. Antimicrobial Tests on Red Ginseng / Golden / Seaweed / Baekhong / Mossy Extracts

1) experimental material

Red ginseng, gold, sancho, Baekduhong and moss used in this experiment were purchased from Eurocostech Co., Ltd., in particular, moss was used as Irish Moss. In order to use the experiment Freeze-dry (Samwon Freezing engineering Co., SFDTS 10K) was used as a sample after removing moisture. In addition, each extraction was used to separate the extract of high concentration and high purity using a supercritical extraction method and hot water / ethanol extraction at the same time.

Sampling Conditions

Ginseng, Golden, Sancho. Baekduhong, moss was lyophilized with Freeze dry (Samwon Freezing engineering Co., SFDTS 10K) and used as a sample after grinding. In addition, 3% (v / v) ethanol was added to extract and extract the functional material of each sample.

2) antibacterial test

Antimicrobial testing was performed on 12 Gram-negative bacteria / 12 Gram-positive bacteria / 5 yeasts / 6 fungi / 33 fungi other than cosmetics.

Antibacterial and antifungal activity of the solvent extract was measured using a solid medium dilution method. [{Experimental Basis} * Experimental Comprehensive Microbiology, University History p.116 (1998)]

Each extract was dissolved in 10% DMSO saline, and the concentration of the extract was performed in a 2-fold dilution step from the highest concentration of 2,000μg / ml to the lowest concentration of 62.5μg / ml. Take 2.0 ml of each diluted sample into Petri dish, mix 18.0 ml of the medium, harden the medium, and incubate the cultured bacteria with platinum for about 5mm, so that bacteria can grow in 37 ℃ incubator for 24 hours and fungi in 22 ℃ incubator. Incubated for ˜7 days. At the end of each incubation, the colony was used to measure the minimum inhibitory concentration of antibiotics that inhibited the growth of microbial strains. [Experimental Basis] * Diagnostic Hospital Microbiology, Korea Medical Science p .589 (1996)]

* Experiment Overview

1) Make a liquid medium using Mueller Hinton broth.

2) Prepare 10 tubes and fill one medium with 4ml and the other 2ml. (Make a badge of 10SET).

3) Make a medium for diluting bacteria.

4) After autoclaving, add antibiotic to the tubes in 2).

5) Transfer 2 ml each from the tube and dilute twice.

6) Dilute the concentration of Escherichia coli and transplant the bacteria from a low concentration tube.

7) Incubate the bacteria in the incubator and observe.

8) Measure the minimum concentration at which bacteria do not grow.

C. Application of Cosmetic Formulations Using Sacral Mushroom, Ginseng, Gold, Sancho, Baekduhong and Moss Extracts

Situation mushrooms, red ginseng, golden, sancho, Baekduhong and moss are all suitable raw materials for cosmetics listed in INCI. Based on the results of experiments, body cleanser based on the level of preservative effect that can replace the existing preservatives of parabens Tested by application to body lotion.

1) Test Content

Situation mushrooms, red ginseng, golden. Sancho, Baekduhong and moss extracts were applied to each body cleanser and body lotion to determine the antimicrobial effect. First, in the solubilizing formulation body cleanser and emulsifying lotion, the control did not add any preservatives, and the test group added 0.5% of each condition mushroom, red ginseng, golden, sancho, baekduhong and moss extracts, and the positive control was a body cleanser / body To the lotion was added 0.1% methyl paraben and 0.1% butyl paraben.

2) test method

The experiment included gram negative bacteria Escherichia coil , Pseudomonas aeruginos , gram positive bacteria Staphylococcus aureus , and fungi Candida albicans . Was used. Tryptic soy was inoculated with Escherichia coil , Pseudomonas aeruginos and Staphylococcus aureus , and siburaud dextrose agar was inoculated. Candida albicans was inoculated. After incubation for 48 hours in a 37 ℃ incubator and suspended in 0.8% physiological saline to 10 ⁸ cells / ml bacteria, 10 ⁶ cells / ml fungi.

10 ml of body cleanser and body lotion to be tested for antimicrobial effect were added and mixed with physiological saline. 1 ml of this solution was taken and mixed in 9 ml of Letheen Broth to make a 10 ^-1 dilution, which was diluted stepwise to make 10 ^-2 , 10 ^-3 , 10 ^-4 dilutions. 10 ^-2 , 10 ^-3 , 10 ^-4 dilutions were inoculated into 1 ml each of Letheen agar plates and incubated in a 37 ° C. incubator for 72 hours to determine the bacterial count.

Body cleanser / body lotion samples inoculated with microorganisms were stored in a 37 ° C. incubator to measure bacterial changes for 4 weeks. The method of measuring the antiseptic power of cosmetics was based on the CTFA method, which is a long-term measurement method of inoculating microorganisms into a product to observe the increase and decrease of the inoculated microorganisms for 28 days.

After the inoculation of bacteria, 0, 1 ~ 3, 7, 14, 21, 28 days after the sample is taken to measure the number of bacteria. There should be no growth. In addition, yeast and mold should be reduced by at least 90% of bacteria within 7 days of inoculation and should not grow during the test period.

Body cleanser prescription ingredient Test group (% by weight) Positive control (% by weight) Situation Mushroom + Ginseng + Golden + Sancho
+ Baekduhong + moss water to 100 to 100 Ammonium Laureth Sulfate 25.00 25.00 Cocamidopropyl Betaine 10.00 10.00 TEA Lauryl Sulfate 10.00 10.00 glycerin 8.00 8.00 Lauramid DEA 3.00 3.00 Steartrimonium Chloride 2.00 2.00 Glycol Distearate 2.00 2.00 PolyQuaternium-10 1.00 1.00 Sodium chloride 1.00 1.00 Shellinus Linteus Extract 1.00 1.00 Pulsatilla Koreana Extract 0.50 - Usnea Barbata (Lichen) Extract 0.50 - Zanthoxylum Piperitum Fruit Extract 0.50 - Golden Extract (ScutellariaBaicalensisRootExtract) 0.50 - Sophora Angustifolia Root Extract 0.50 - Diospyros kaki leaf Extract 0.50 0.50 Spices (Naucos, Inc. contains galacsolide, hedion, linalool) 0.20 0.20 Citric Acid 0.10 0.10 Disodium EDTA 0.03 0.03 Methyl paraben - 0.10 Butyl paraben - 0.10

 Prescription of body lotion ingredient Test group (% by weight) Positive control Situation Mushroom + Ginseng + Golden + Sancho
+ Baekduhong + moss water to 100 to 100 Dipropylene glycol 10.00 10.00 Mineral oil 5.00 5.00 Hexyl laurate 3.00 3.00 Stearic acid 2.50 2.50 Polysorbate 60 2.00 2.00 PEG / PPG-17 / 6 Copolymer 2.00 2.00 Stearyl alcohol 2.00 2.00 Dimethicone 1.50 1.50 Sorbitan sesquioleate 1.50 1.50 Glyceryl stearate 1.50 1.50 PEG-100 Stearate 1.00 1.00 Shellinus Linteus Extract 1.00 1.00 Pulsatilla Koreana Extract 0.50 - Usnea Barbata (Lichen) Extract 0.50 - Zanthoxylum Piperitum Fruit Extract 0.50 - Golden Extract (ScutellariaBaicalensisRootExtract) 0.50 - Sophora Angustifolia Root Extract 0.50 - Diospyros kaki leaf Extract 0.50 0.50 Spices (Naucos, Inc. contains galacsolide, hedion, linalool) 0.15 0.15 Triethanolamine 0.15 0.15 Carbomer 0.13 0.13 Allantoin 0.10 0.10 Xanthan gum 0.05 0.05 Disodium EDTA 0.03 0.03 Methyl paraben - 0.1 Butyl paraben - 0.1

2. Results

A. Antioxidant Activity Test Results

1) DPPH (1,1-Diphenyl-2-picrylhydrazyl radical scavenging) activity measurement result

DPPH radicals are relatively stable free radicals and are widely used to search for antioxidant activity of natural resources. According to the extraction conditions, 20 μl of the extracts were added to measure the activity, and the hot water and ethanol extracts had higher activity than the supercritical ones. It was relatively higher than the supercritical extraction.

DPPH radical scavenging activity Sample No. DPPH radical scavenging activity (%) Sample No. DPPH radical scavenging activity (%) One 25.8 13 36.9 2 34.6 14 22.7 3 25.1 15 31.6 4 32.3 16 43.7 5 43.5 17 31.9 6 38.8 18 31.9 7 44.6 19 33.5 8 44.1 20 25.4 9 30.5 21 34.8 10 53.8 22 36.4 11 91.3 12 83.4

2) ABTS radical scavenging activity measurement results

Determination of antioxidant activity using ABTS radicals was performed by measuring the ABTS free radicals produced by the reaction with potassium persulfate by the antioxidants in the extracts, which resulted in decolorization of the index-specific turquoise blue color. , As shown in Table 5, 227.4 ~ 458.4 µg / ml for each extract. The supercritical extraction condition showed the highest radical activity at 300bar and 60 ℃ and the lowest activity at 150bar and 40 ℃ with the lowest pressure and temperature.

ABTS radical scavenging activity Sample No. ABTS radical scavenging activity (%) Sample No. ABTS radical scavenging activity (%) One 227.4 13 421.4 2 281.6 14 244.9 3 234.0 15 348.7 4 283.0 16 458.5 5 373.1 17 356.1 6 349.1 18 349.1 7 368.0 19 370.2 8 394.2 20 279.0 9 243.2 21 384.6 10 475.9 22 425.4 11 778.1 12 779.4

3) Superoxide radical scavenging (SOD) activity measurement result

Superoxide (O ₂ ), one of the noxious oxygen species that occurs during biometabolism, is eliminated by superoxide dismutase (SOD). SOD activity was positively correlated with the concentration of 2, 4, 6, 8 μl of supercritical mushroom extract. Particularly, when the extract concentration was 8 μl, the SOD activity was remarkably high at 7, 8, and 9 times, and the extraction condition was significantly affected. SOD activity was determined as 15.8? It showed high activity of 59.1%. Sample no. In supercritical extract It showed relatively high activity at 7, 8 and 9. In particular, the 250bar / 60 ℃ conditions showed the highest activity during the supercritical extraction, 300 bar was higher activity at 40 ℃ than 50 ℃ was higher effect at lower temperatures at high pressure.

SOD activity according to the concentration of extract Sample No. Concentration (μl) One 2 3 4 5 6 7 8 9 10 11 0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 2 4.5 7.0 6.7 6.6 15.9 15.6 24.2 17.5 19.7 9.8 7.9 4 8.4 12.5 11.5 13.7 23.8 18.5 41.7 32.5 34.3 18.6 13.9 6 11.2 17.6 15.7 17.5 29.5 24.5 50.8 44.5 41.7 23.1 20.1 8 15.8 22.9 20.5 22.2 37.6 31.4 59.2 53.9 48.0 27.3 28.4

4) Consideration of the results of the situation mushroom antioxidant activity test

As described above, the situation mushroom extract is judged to have an excellent antioxidant activity compared to the antioxidant activity of DPPH 50 ~ 60% of commonly known green tea. In addition, by examining the compounds with various physiological activities of the situation mushroom, it is considered that the cosmetics are of sufficient value as an antioxidant material.

B. Antimicrobial and Antiseptic Test Results of Red Ginseng, Golden, Sancho, Baidu and Moss Extracts

Each extract of Gosam, Golden, Sancho, Baekduhong, and Moss was dissolved in 10% DMSO saline, and the concentration of the extract was doubled from the highest concentration of 2,000μg / ml to the lowest concentration of 62.5μg / ml. Take 2.0 ml of each diluted sample into Petri dish, mix 18.0 ml of the medium, harden the medium, and incubate the cultured bacteria with platinum for about 5mm, so that bacteria can grow in 37 ℃ incubator for 24 hours and fungi in 22 ℃ incubator. Incubated for ˜7 days.

1) Gram negative bacteria / gram positive bacteria minimum inhibitory concentration (MIC) test results

Gram negative bacteria MIC
(ppm)  Gram-positive bacteria MIC
(ppm)  Escherichia coli 260  Bacillus subtilis 370  Pseudomonas aeruginosa 340  Bacillus cereus 350  Salm. typhimurium 360  Clostridium botulinum 360  Salm. pullorum 300  Lactobacillus arabinosus 520  Aerobacter aerogenes 270  Staphylococcus aureas 410  Proteus vulgaris 320  Listeria monocytogenes 410  Vibrio cholerae 350  Corynebacterium acnes 480  Cloaca cloacae 340  Diplococcus pneumoniae 440  Klebsiella edwardsii 280  Mycobacterium smegmatis 380  Legionella pneumoniae 360  Sarcina lutea 370  Moraxella duplex 310  Streptococcus agalactiae 430  Shigella flexneri 320  Clostridium tetani 370

2) MIC test result of yeast / fungus

 East MIC
(ppm) Fungus MIC
(ppm)  Candida albicans 480  Aspergillus niger 890  Saccharomyces cerevisiae 450  Aspergillus fumigatus 810  Rhodotorula minuta 400  Penicillium citrinum 830  Rhodotorula mucilaginosa 430  Fusarium monliforme 720  Rhodotorula texensis 430  Aureobasidium pullulans 870  Chaetomuim globosum 730

3) MIC test results of other microorganisms

Bacteria (300-600 ppm) Yeast and fungus
(500-1000ppm) Aerobacter spp. Proteus vulgaris Aspergilus flavus Bacillus mycoikes Shigella dysenteriae Aspergilus oryzae Brucella abortus Salmonella anatum Epidermophyton floccosum Brevibacterium ammoniagenes Salmonella choleraesuis Epidermo phyton Corynebacterium
acnesanaerobic Salmonella typhi Poria spp. Clostridium spp Salmonella schorttmuelleria Pulluaria pullulans Erwinia Staphylococcus pyogenes Trichophyton rubrum Hycobacterium Staphylococcus
saprophylicus Trichophyton interdigtal Listeria multocida Staphylococcus
epidermidis Trichophyton gypseum Lipophilic diptheroid strain Staphylococcus pyogenes Keratinomyces ajelloi Mima polymorpha Staphylococcus faecalis Monilia albicans

4) Review of Antimicrobial and Antiseptic Test Results of Red Ginseng, Golden, Sancho, Baidu and Moss Extracts

When using raw materials of Sancho / Baekduhong / Moss, the growth and suppression of the bacteria are less than the number of proper bacteria notified by the KFDA. Therefore, the replacement effect of the existing parabens synthetic preservatives and the commercialization of the product can be expected. It is considered to be.

C. Application of Cosmetic Formulations Using Sacral Mushroom, Gosam, Golden, Sancho, Baekduhong and Moss Extracts

1) Antibacterial activity against Escherichia coli

The antimicrobial activity of S. mushroom / Ginseng / Golden / Seaweed / Baekhong-hong / Mossy extract against the Gram-negative bacterium Escherichia coli is as follows.

(A) Body Cleanser Number of cells (cell / ml) 3 days 1 week 2 weeks 3 weeks 4 weeks control 2,310,000 3,000 1,000 100 100 Test group 1,800,000 0 0 0 0 Positive control 1,200,000 0 0 0 0

(B) body lotion Number of cells (cell / ml) 3 days 1 week 2 weeks 3 weeks 4 weeks control 2,280,000 9,600 5,200 100 100 Test group 710,000 0 0 0 0 Positive control 520,000 0 0 0 0

(A) Escherichia coli was most present on the third day in the control group without preservatives in the body cleanser, and survived up to 4 weeks. In the positive control group with 0.1% methyl paraben and 0.1% butyl paraben, almost half of the fungi were killed on day 3, and 1% by weight of mushroom extract and 0.5% by weight of red ginseng, golden, sancho, Baekduhong and moss extracts were added. In one test group, the bacteria remained about the same as the number of bacteria in the positive control group on the third day of addition.

(B) Escherichia coli was the most common on day 3 of the control group without preservative in body lotion and survived until 4 weeks. In the positive control group, the bacteria began to decrease on the third day and the bacteria were completely killed from the first week. In contrast, the number of bacteria in the test group was similar to that of the positive control group from the 3rd day. From week 1, all bacteria were killed as in the positive control group.

The test group added 1% by weight of situation mushroom extract and 0.5% by weight of red ginseng, golden, sancho, Baekduhong and moss extracts in both solubilized and emulsified formulations. It can be seen that the antibacterial activity is similar to that of the positive control group added.

2) Antimicrobial Activity against Pseudomonas aeruginos

The antimicrobial activity of Pseudomonas aeruginos , a Gram-negative bacterium, of Pseudomonas aeruginos was measured as follows.

(A) Body Cleanser Cell count (cell / ml) 3 days 1 week 2 weeks 3 weeks 4 weeks control 220,000 34,000 9,000 3000 0 Test group 60,000 0 0 0 0 Positive control 76,000 0 0 0 0

(B) body lotion Cell count (cell / ml) 3 days 1 week 2 weeks 3 weeks 4 weeks control 480,000 190,000 36,000 1,000 1,000 Test group 2,000 0 0 0 0 Positive control 7,000 0 0 0 0

As can be seen from the above experimental results, Pseudomonas Pseudomonas The results of the antimicrobial activity against aeruginos ) were almost the same as those of the positive control group, but the antimicrobial activity was higher in the test group containing 1% by weight of S. mushroom extract and 0.5% by weight of red ginseng, golden, sancho, Baekduhong and moss extracts. It was found to be high.

3) Antibacterial activity against Staphylococcus aureus

The antimicrobial activity of S. mushroom / Ginseng / Golden / Seaweed / Baekhong / Moss extract against Gram-positive bacteria Staphylococcus aureus was as follows.

(A) Body Cleanser Cell count (cell / ml) 3 days 1 week 2 weeks 3 weeks 4 weeks control 270,000 3,100 500 100 100 Test group 6,000 0 0 0 0 Positive control 4,000 0 0 0 0

(B) body lotion Cell count (cell / ml) 3 days 1 week 2 weeks 3 weeks 4 weeks control 2,200,000 14,000 1,100 100 100 Test group 20,000 0 0 0 0 Positive control 30,000 0 0 0 0

As can be seen from the experimental results, in the body cleanser, Staphylococcus aureus was most present on the third day in the control group without the addition of preservatives, and even up to the fourth week. On the other hand, in the test group and the positive control group, the bacteria started to be markedly killed from the third day and completely killed from the first week.

In addition, the body lotion was found to have the most staphylococcus aureus on the third day in the control group without preservatives, even up to 4 weeks. As with the body cleanser, in the body lotion, bacteria were significantly killed from the 3rd day in the test group and the positive control group, and completely killed from the 1st week.

4) Antimicrobial activity against Candida albicans

The antimicrobial activity of the extract of red ginseng / gold / sancho / baekdu / moss against the fungus Candida albicans was as follows.

(A) Body Cleanser Cell count (cell / ml) 3 days 1 week 2 weeks 3 weeks 4 weeks control 190,000 5,300 600 400 200 Test group 73,000 0 0 0 0 Positive control 89,000 0 0 0 0

(B) body lotion Cell count (cell / ml) 3 days 1 week 2 weeks 3 weeks 4 weeks control 210,000 4,400 800 100 0 Test group 62,000 0 0 0 0 Positive control 58,000 0 0 0 0

As can be seen from the experimental results, the control group did not kill bacteria until 4 weeks in the body cleanser and body lotion, whereas all Candida albicans were killed in 1 week in the test group and the positive control group. From the above results, it was confirmed that the test group had excellent antibacterial activity against Candida albicans similar to the positive control group.

3. Conclusion

This experiment was conducted to develop a safer and less dangerous natural antibacterial agent for removing odor from cosmetics used by the elderly. As a result, according to the present invention, the cosmetic composition containing the situation mushroom, red ginseng, yellow root, Sancho, Baekduhong, and moss extract showed an antimicrobial / preservative effect similar to the cosmetics containing parabens. It is expected to be fully expected.

Claims (5)

Natural herbal cosmetic composition for removing the elderly odor, characterized by containing a situation mushroom extract, red ginseng extract, golden extract, sancho extract, Baidu extract and moss extract.
According to claim 1, The extract of the situation mushroom natural herbal cosmetic composition for removing the elderly odor, characterized in that it comprises 0.5-1.5% by weight relative to the total weight of the cosmetic composition on a dry weight basis.
According to claim 1, The ginseng extract, golden extract, Sancho extract, Baekduhong extract and moss extract is 0.2-1% by weight based on the dry weight of the total weight of the cosmetic composition, characterized in that the natural odor removal for the elderly Herbal cosmetic composition.
According to claim 1, wherein each extract is a natural herbal cosmetic composition for removing the elderly odor, characterized in that obtained by at least one extraction method of supercritical extraction method, hot water extraction method and ethanol extraction method.
According to claim 1, wherein the cosmetic composition is a natural herbal cosmetic composition for removing odors, characterized in that the liquid, cream, paste or solid phase.