CN106138122A - Herba Limonii Gmelinii extract and its production and use - Google Patents
Herba Limonii Gmelinii extract and its production and use Download PDFInfo
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- CN106138122A CN106138122A CN201510158305.XA CN201510158305A CN106138122A CN 106138122 A CN106138122 A CN 106138122A CN 201510158305 A CN201510158305 A CN 201510158305A CN 106138122 A CN106138122 A CN 106138122A
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Abstract
The invention discloses Herba Limonii Gmelinii extract and its production and use.The preparation method of this Herba Limonii Gmelinii extract comprises the steps: that Herba Limonii Gmelinii is mixed by (1) with solvent, extracts, and filters, removed under reduced pressure solvent, i.e. can get Herba Limonii Gmelinii crude extract；(2) after Herba Limonii Gmelinii crude extract being dissolved in water, it is loaded to resin packed column, successively with water and ethanol solution eluting, collects eluent respectively, removed under reduced pressure second alcohol and water,.The preparation method of the Herba Limonii Gmelinii extract of the present invention is simple, and the Herba Limonii Gmelinii extract prepared can be as tyrosinase inhibitor, elastase inhibitor, antioxidant activity agent and the application of anti-aging actives；The further preferably application in preparing skin care item or cosmetics.
Description
Technical field
The present invention relates to field of plant extraction, be specifically related to a kind of Herba Limonii Gmelinii extract and preparation method thereof and
Purposes.
Background technology
Within 1956, Harman proposes free radical causes of senescence [Harman, D.Aging:A theory based
On free radical and radiation chemistry.J.Gerontol.1956,11:289-300.], it is thought
Too much free radical is the major reason causing biological aging.Theoretical according to this, the activity of internal excess
Oxygen-derived free radicals and unsaturated fatty acid effect produce the materials such as malonaldehyde, malonaldehyde by with the egg on cell membrane
The effect such as white matter produces brown pigment, is deposited in skin and becomes as various mottles.The free radical of excess can also make
Collagen fiber in skin, elastic fiber crosslink with degeneration, become fragile, follow the string, work as skin beauty water
When dividing not enough, easily make elastic fibers break, the skin ageing phenomenons such as dark stricture of vagina, microgroove, wrinkle occur.
Additionally, the ionizing radiation in environment and the environmental contaminants of such as air pollution and chemical substance etc., also
Free radical is constantly produced in making organism.Such as ultraviolet in environment can make the fibre in dermis of skin
Dimension blast cell and grain wire body are upset, and then discharge superoxide anion, and the superoxide anion of excess
Then can be converted into other destructive higher free radical.It is able to maintain that oxidation and antioxygen although having in human body
The Antioxidative Defense System of poised state between change, in order to slow down the generation of active oxygen and free radical, if but
Be the most excessive to tan by the sun in the sun, then in human body, a large amount of free radicals produced can cause the antioxygen of skin
Change defence capability to reduce, cause the injury of skin, such as photoaging, creasy surface, skin immunization power to lose
Adjust.Antioxidant is the material having and catching free radical ability, can remove the living radical in skin,
Alleviate the skin oxidative stress damage that free radical causes, thus it is old to improve the skin caused by oxidative stress etc.
Change.The removing free radical type antioxidant being currently known in the organism such as vitamin E, vitamin C,
The most also (the patent documentations such as the antioxidant such as Flos Nelumbinis of plant origin, prunus mume (sieb.) sieb.et zucc. berry extract are reported
CN201110158540.9 contains Flos Nelumbinis extract and Fructus Mume extract and has antioxygen as effective ingredient
Change the Dermatologic preparation composition of activity).
About the formation of dermal melanin, current study show that mainly due to the bioid in melanocyte
Reaction causes, and i.e. tyrosine is under the effect as the tryrosinase of catalyst, generates DOPA quinone, then
Melanin is formed by the catalytic action of enzyme or the Oxidation of non-enzymatic.Generate about suppressing above-mentioned melanin
Whitening active thing, generally with suppression tyrosinase activity as major target class.Derive from plant extract
Tyrosinase inhibitor, as potential whitening active thing, has again safety and stimulation low to skin simultaneously
Property expection, the at present report of existing various exploitations, such as patent documentation CN201310411195.4 is white
The detection method of liana extract white-skinned face function；CN201310382406.6 Lignum Aquilariae Resinatum peel extract is in system
Application in standby skin-lightening cosmetic.
It is aging that the reason of skin aging can be divided into that photoaging and endogenous cause of ill cause, and UV exposes the light caused
Aging meeting causes the activity increase of dermal elastin enzyme.Elastoser (Elastase) is distributed in multiple
In tissue and cell, elastase activity increase may result in the hydrolysis of elastic fibers of skin, thus accelerates
Intradermal collagen protein and the decomposition of elastin laminin, make skin follow the string, the skin aginges such as wrinkle occur
Phenomenon.The anti-aging active thing being currently known plant origin has Elastase Inhibitory, such as knotweed
Blue (patent documentation CN200680023570.6 skin preparations for extenal use), sweet pea (patent documentation
CN200680009020.9 contains the cosmetic preparation of sweet pea extract) etc..
Herba Limonii Gmelinii (Limonium sinense (Girard) Kuntze) is Plumbaginaceae (Plumbaginaceae)
The flower of Statice (Limonium Mill.) Herba Limonii Gmelinii or herb.Herba Limonii Gmelinii another name cry again sweet heart careless,
One prunus mume (sieb.) sieb.et zucc., sea Radix Paeoniae Rubra, spend that beautiful money is fragrant, limonium sinenseKuntze (" Chinese Higher plant illustrated handbook ") in vain.Enrich blood
The main chemical compositions of grass includes tannin, flavone, anthocyanidin etc., such as Myricitrin, rutin, poplar
Prunus mume (Sieb.) Sieb. Et Zucc skin element, Quercetin, kaempferol etc..The main pharmacological of Herba Limonii Gmelinii be antiinflammatory, pain relieving,
Invigorate blood circulation, enrich blood, regulating menstruation, hemostasis etc., in time spending and just open, gather herb using fresh herb or dry use." Chinese Plants
Will " to describe Herba Limonii Gmelinii be Kazak traditional national medicine, the root of Herba Limonii Gmelinii or herb among the people for stopping blooding,
Convergence and diuretic.Xinjiang flora Chinese herbal medicine is recorded and sweet heart's grass seeds is spent as family at flowerpot.Herba Limonii Gmelinii because of
Its flower is tiny, dry film matter, and color is simple and elegant, and period of viewing and admiring is long, is important to join floral material.Herba Limonii Gmelinii
Plant is the highest, and spray is the longest, can not wither, have purple, pale purple, Flos Rosae Rugosae powder, indigo plant, red, white,
Yellow assorted kind, is referred to as " Myosotis sylvatica ", " Myosotis sylvatica " flower, dry flower and the scented tea on market,
It it is not the plant Myosotis sylvatica (Myosotis silvatica Ehrh.ex Hoffm.) of Boraginaceae myosotis
Flower, be essentially all Limonnium Herba Limonii Gmelinii (Limonium sinense (Girard) Kuntze) or
The flower of Herba Limoii Bicoloris (Limonium bicolor (Bag.) Kuntze).
Summary of the invention
The technical problem to be solved there is provided a kind of Herba Limonii Gmelinii extract and preparation method thereof
And purposes.The preparation method of the Herba Limonii Gmelinii extract of the present invention is simple, and the Herba Limonii Gmelinii extract prepared is permissible
As tyrosinase inhibitor, elastase inhibitor, antioxidant activity agent and anti-aging actives
Application；The further preferably application in preparing skin care item or cosmetics.
The invention provides the preparation method of a kind of Herba Limonii Gmelinii extract, it is any that it uses in following method
One,
Method one, comprise the steps: to mix Herba Limonii Gmelinii with solvent, extract, filter, removed under reduced pressure
Solvent,；
Method two, comprise the steps:
(1) Herba Limonii Gmelinii is mixed with solvent, extract, filter, removed under reduced pressure solvent, i.e. can be mended
Blood grass crude extract；
(2), after Herba Limonii Gmelinii crude extract being dissolved in water, it is loaded to resin packed column, successively by water and second
Alcoholic solution eluting, collects eluent respectively, removed under reduced pressure second alcohol and water,.
Wherein, described Herba Limonii Gmelinii is generally Statice (Limonium Mill.) plant, preferably
Statice Herba Limonii Gmelinii (Limonium sinense (Girard) Kuntze), Herba Limoii Bicoloris (Limonium
Bicolor (Bag.) Kuntze), L. kaschgarieum (Limonium kaschgaricum (Rupr.) Ik.-Gal.),
Limonium gmelinii (Wildl.) Kuntze (Limonium gmelinii (Willd.) Kuntze), L. drepanostachyum (Limonium
Drepanostachyum Ik.-Gal.), Limonium flexuosum (Limonium flexuosum (L.) Kuntze), wood
This Herba Limonii Gmelinii (Limonium suffruticosum (L.) Kuntze), Limonium roborowskii Ik.-Gal. (Limonium
Roborowskii Ik.-Gal.), Limonium franchetii (Limonium franchetii (Debx.) Kuntze), Corallium Japonicum Kishinouye
Herba Limonii Gmelinii (Limonium coralloides (Tausch) Lincz.), Limonium chrysocomum kuntze (Limonium
Chrysocomum (Kar.&Kir.) Kuntze), L. leptolobum (Limonium leptolobum (Regel)
Kuntze), Limonium myrianthum Kuntz (Limonium myrianthum (Schrenk)), Limonium wrightii Kuntz (Limonium
Wrightii (Hance) Kuntze), Limonium tenellum Kuntz (Limonium tenellum (Turcz.) Kuntze), ear
Leaf Herba Limonii Gmelinii (Limonium otolepis (Schrenk) Ktze.) and Herba Limonii Gmelinii with yellow flower (Limonium aureum
(L.) Hill) in one or more, be more preferably Statice Herba Limonii Gmelinii (Limonium sinense
(Girard)Kuntze)。
Wherein, in step (1), described extraction is preferably supersound extraction, stirring is extracted or adverse current
Circulation is extracted, and the time of described supersound extraction is preferably 0.5～1.5 hour, and described stirring is extracted
Time be preferably 1.5～2 hours, it is little that the time that described circulated in countercurrent is extracted is preferably 0.3～1
Time.
Wherein, in step (1), described preferred solvents ground is ethanol that percent by volume is 60～80%
Aqueous solution.
Wherein, the volume mass ratio preferably 10～40mL/g of described Herba Limonii Gmelinii and solvent.
Wherein, in step (2), described ethanol solution eluting is preferably successively by 10%～30% second
Alcohol-water solution, 31%～50% ethanol water, 51%～70% ethanol water, 71%～90% ethanol water
Solution and 91%～99% ethanol water carry out Concentraton gradient eluting, are more preferably to use 20% ethanol successively
Aqueous solution, 40% ethanol water, 60% ethanol water, 80% ethanol water and 95% ethanol water
Solution carries out Concentraton gradient eluting；Percentage ratio is percent by volume.
Wherein, described Herba Limonii Gmelinii can be Herba Limonii Gmelinii whole plant, including showy flowers of herbaceous plants and/or the Herba Limonii Gmelinii herb of enriching blood.
Wherein, described resin can be macroporous resin commonly used in the art, the most commercially available
D101 resin or AB-8 resin.
Wherein, described water is preferably deionized water.
Present invention also offers the Herba Limonii Gmelinii extract obtained by above-mentioned preparation method, described Herba Limonii Gmelinii carries
Take the extract that thing preferably 31%～50% ethanol water eluting obtains, be more preferably 40% ethanol
The extract that aqueous solution affords；Percentage ratio is percent by volume.
Present invention also offers above-mentioned Herba Limonii Gmelinii extract to press down as tyrosinase inhibitor, elastoser
Preparation, antioxidant activity agent or the application of anti-aging actives；Further preferably preparing skin care item or change
Application in cosmetic.
On the basis of meeting common sense in the field, above-mentioned each optimum condition, can combination in any, i.e. get Ben Fa
Bright each preferred embodiments.
Agents useful for same of the present invention and raw material are the most commercially.
The most progressive effect of the present invention is: the preparation method of the Herba Limonii Gmelinii extract of the present invention is simple,
Prepare Herba Limonii Gmelinii extract can as non-diseases diagnosis and/or the tyrosinase inhibitor of therapeutic use,
Elastase inhibitor, antioxidant activity agent and the application of anti-aging actives, can carry Herba Limonii Gmelinii
Take thing to coordinate in external preparation, as preventing skin ageing, maintaining skin state anti-ageing of young healthy
Agent uses.
Detailed description of the invention
Further illustrate the present invention below by the mode of embodiment, but the most therefore limit the present invention to
Among described scope of embodiments.The experimental technique of unreceipted actual conditions in the following example, according to often
Rule method and condition, or select according to catalogue.
Unless otherwise indicated, the percentage ratio in following embodiment is percent by volume.
Embodiment 1
The preparation of Herba Limonii Gmelinii extract
By Herba Limonii Gmelinii whole plant (Statice Herba Limonii Gmelinii (Limonium sinense (Girard) commercially available for 100g
Kuntze) flower, stem and leaf and root) crushed after being dried, with 2000mL 70% ethanol solution (v/v, this
In bright, all ethanol solution are volumetric concentration) mixing immersion, ultrasound assisted extraction 1 hour, filter,
Filtrate, after removed under reduced pressure solvent, obtains Herba Limonii Gmelinii crude extract 20g.
Take Herba Limonii Gmelinii crude extract, after deionized water dissolving, be loaded to commercially available AB-8 resin packed column,
Successively with deionized water, 20% ethanol, 40% ethanol, 60% ethanol, 80% ethanol and 95% ethanol ladder
Degree eluting (percentage ratio is percent by volume), collects eluent, removed under reduced pressure second alcohol and water respectively, claims
Weight, respectively obtains water elution position 11g, 20% alcohol elution 2.0g, 40% alcohol elution
2.2g, 60% alcohol elution 2.1g, 80% alcohol elution 1.1g and 95% alcohol elution
The Herba Limonii Gmelinii extract of 1.0g.
Embodiment 2
The mensuration of general flavone content in Herba Limonii Gmelinii extract
Herba Limonii Gmelinii extract embodiment 1 prepared, according to Pharmacopoeia of People's Republic of China (2010 editions)
Method, with rutin as standard substance, measures general flavone content in Herba Limonii Gmelinii extract.
Determination step is: 1) standard curve making: precision measure standard solution 1ml, 2ml, 3ml,
4ml, 5ml, 6ml, put in 25ml measuring bottle respectively, respectively adds 30% ethanol to 6.0ml, adds 5% nitrous
Acid sodium solution (mass percent concentration) 1ml.Mixing, places 6 minutes, then it is molten to add 10% aluminum nitrate
Liquid (mass percent concentration) 1ml, shakes up, and places 6 minutes.Hydro-oxidation sodium test solution 10ml, then
Add 30% ethanol to scale, shake up, place 15 minutes, with corresponding reagent as blank, use ultraviolet-visible
Spectrophotography, measures absorbance at the wavelength of 510nm, and with absorbance as vertical coordinate, concentration is horizontal
Coordinate, draws standard curve.
2) the Herba Limonii Gmelinii extract at each eluting position that Example 1 prepares is completely dissolved in deionized water,
It is configured to 1mg/mL aqueous solution.Being placed in 25ml volumetric flask by a certain amount of sample, other is according to standard
Curve preparation process, from " adding 5% sodium nitrite solution 1ml ", operates same step 1), surveys successively
Determining absorbance, take sample solution 3ml simultaneously, in addition to being not added with sodium hydroxide test solution, remaining ibid operates,
As blank, calculate general flavone content (in terms of rutin) in Herba Limonii Gmelinii extract, as shown in table 1 below.
Table 1 Herba Limonii Gmelinii extractive total flavone content
From table 1, the crude extract obtained after Herba Limonii Gmelinii is extracted contains more flavone compound,
Flavone is the main active of Herba Limonii Gmelinii, after adsorbent resin separates, and Herba Limonii Gmelinii 40% ethanol elution portion
The flavone of position enrichment is most, improves a lot than flavones content in crude extract, and its biological activity increases the most accordingly
By force.
Embodiment 3
The detection of tyrosinase inhibitory activity
The Herba Limonii Gmelinii extract at each eluting position embodiment 1 prepared is completely dissolved in deionized water, preparation
Become 1.0mg/mL aqueous solution, i.e. sample solution, be used for measuring tyrosinase inhibitory activity, with arbutin
For object of reference.
Determination step is: phosphate buffered solution (pH6.8,30mM) 70 μ L, 1.66mM tyrosine
Solution 80 μ L, sample solution 80 μ L mix homogeneously, incubation more than 5 minutes in 37 DEG C of thermostatic water bath,
Add tyrosinase solution (125U/ml) 10 μ L, after 37 DEG C of constant temperature 10min, measure by microplate reader
The absorbance A of 475nm wavelength475.Sample aqueous solution is replaced with deionized water, same as reference solution
Measure absorbance, the results are shown in Table 2.
Inhibitory activity against tyrosinase computing formula is: suppression ratio (%)=(A0-(A475-B))/A0
× 100%, wherein A0It is the absorbance of reference solution, A475Being the absorbance of sample solution, B is sample
The absorbance of product blank solution.
The measurement result of table 2 tyrosinase inhibitory activity
From table 2, the crude extract obtained after Herba Limonii Gmelinii is extracted has the suppression to tyrosinase activity
Effect, after adsorbent resin separates, the alcohol elution of gained all can realize tyrosinase activity
Suppression, and be greatly enhanced than the inhibitory action of crude extract.Wherein Herba Limonii Gmelinii 40% alcohol elution pair
The inhibitory action of tyrosinase activity is the strongest.
Embodiment 4
The detection of antioxidant activity
The Herba Limonii Gmelinii crude extract prepared by embodiment 1 method and extract, with DPPH (1,1-bis-
Phenyl-2-trinitrophenyl-hydrazine) method measure its remove free radical antioxidant activity.Determination step is: will enrich blood
Grass extract is configured to 0.01mg/mL aqueous solution with deionized water respectively, pipettes 2mL and has in 10mL
In plug test tube, add 2mL DPPH ethanol solution (2 × 10-4Mol/L), fully mixing, room temperature stands
By the absorbance A of spectrophotometric determination 517nm wavelength after 30min517；Measure 2mL to extract simultaneously
Thing solution and the mixed absorbance A of 2mL ethanol0, 2mL water mixes with 2mL DPPH ethanol solution
After absorbance C and 2mL water and 2mL ethanol solution mixed absorbance C0.Parallel assay three
Secondary, to average, calculate free radical scavenging activity according to below equation, clearance rate is the biggest, and extract is described
Oxidation resistance the strongest.
Free radical scavenging activity (%)=[1-(A517-A0)/(C-C0)] × 100%
The testing result of table 3 DPPH method free-radical scavenging activity
From table 3, the crude extract obtained after Herba Limonii Gmelinii is extracted, can realize DPPH free radical
Scavenging action, after adsorbent resin separates, gained alcohol elution all can realize DPPH free radical
Removing, wherein Herba Limonii Gmelinii 40% alcohol elution has the biggest compared with the scavenging action of crude extract
Improving, it removes concentration SC to the half of free radical50For 0.005mg/mL.
Embodiment 5
According to the Herba Limonii Gmelinii extract of embodiment 1 preparation, it is dissolved in deionized water, is configured to 1.0mg/mL
Aqueous solution, is sample solution, is used for measuring elastase inhibitory activity.
Assay method enters according to document (Am.J.Pharmacol.Toxicol., 2009,4,127-129) method
OK, determination step is: add 10 μ L sample solution in 96 orifice plates and 130 μ L contain 1.015mM
The 0.1M Tris-HCl buffer solution of reaction substrate Succ-Ala-Ala-Ala-p-nitroanilide
(pH8.0), incubation 5 minutes at 25 DEG C, add 15 μ L elastin laminin enzymatic solution (0.5U/mL),
Continue incubation 30min at 25 DEG C, then measure the absorbance A of 410nm wavelength by microplate reader410.With
Deionized water replaces sample aqueous solution, measures absorbance as reference solution equally, the results are shown in Table 4.
Elastase activity suppression ratio computing formula is: suppression ratio (%)=(A0-(A410-B))
/A0× 100%, wherein A0It is the absorbance of reference solution, A410Being the absorbance of sample solution, B is
The absorbance of sample blank solution.
The measurement result of table 4 elastase activity suppression
As shown in Table 4, the Herba Limonii Gmelinii extract used in the present invention is respectively provided with suppression elastoser and makees
With, wherein Herba Limonii Gmelinii 40% alcohol elution suppression elastoser effect is the strongest, therefore can will mend
Blood grass extract coordinates in external preparation, as preventing skin ageing, the skin state of maintenance young healthy
Age resister use.
Embodiment 6
Anti-aging active is tested
It is configured to quality hundred according to the Herba Limonii Gmelinii 40% alcohol elution deionized water of embodiment 1 preparation
Proportion by subtraction is 0.02% solution, joins in human fibroblasts culture fluid, with the deionized water without sample
For blank.After cultivating 48 hours, take a part of cell sample at random, after cell dyeing, use
Microplate reader measures absorbance at 475nm, assesses the proliferation function to human fibroblasts, blank
The rate of increase is 100%, if the rate of increase is higher than 100% for cell is had proliferation function.Take remaining cell sample
This, measure the generation of kit measurement collagen protein by I-type collagen, and the increment rate of blank is
100%, if increment rate promotes collagen protein nucleus formation more than 100% for having.
Table 5 anti-aging active is tested
As shown in Table 5, between blank and Herba Limonii Gmelinii extract, observed anti-aging active statistics
Learning significant difference, Herba Limonii Gmelinii 40% alcohol elution that display uses in the present invention is to human desmocyte
Cell has good proliferation function, has the activity promoting that I-type collagen generates.Therefore, it can
Herba Limonii Gmelinii extract is coordinated in skin preparations for extenal use, as preventing skin ageing, maintaining young healthy
The age resister of skin state uses.
Claims (10)
1. a preparation method for Herba Limonii Gmelinii extract, its use in following method any one,
Method one, comprise the steps: to mix Herba Limonii Gmelinii with solvent, extract, filter, removed under reduced pressure
Solvent,；
Method two, comprise the steps:
(1) Herba Limonii Gmelinii is mixed with solvent, extract, filter, removed under reduced pressure solvent, i.e. can be mended
Blood grass crude extract；
(2), after Herba Limonii Gmelinii crude extract being dissolved in water, it is loaded to resin packed column, successively by water and second
Alcoholic solution eluting, collects eluent respectively, removed under reduced pressure second alcohol and water,.
2. preparation method as claimed in claim 1, it is characterised in that described Herba Limonii Gmelinii is for enriching blood
Grass belongs to (Limonium Mill.) plant, preferably Statice Herba Limonii Gmelinii (Limonium sinense
(Girard) Kuntze), Herba Limoii Bicoloris (Limonium bicolor (Bag.) Kuntze), L. kaschgarieum
(Limonium kaschgaricum (Rupr.) Ik.-Gal.), Limonium gmelinii (Wildl.) Kuntze (Limonium gmelinii
(Willd.) Kuntze), L. drepanostachyum (Limonium drepanostachyum Ik.-Gal.), bent branch enrich blood
Grass (Limonium flexuosum (L.) Kuntze), Limonium suffruticosum Kuntz (Limonium suffruticosum (L.)
Kuntze), Limonium roborowskii Ik.-Gal. (Limonium roborowskii Ik.-Gal.), Limonium franchetii (Limonium
Franchetii (Debx.) Kuntze), Limonium coralloides Lincz (Limonium coralloides (Tausch) Lincz.),
Limonium chrysocomum kuntze (Limonium chrysocomum (Kar.&Kir.) Kuntze), L. leptolobum
(Limonium leptolobum (Regel) Kuntze), Limonium myrianthum Kuntz (Limonium myrianthum
(Schrenk)), Limonium wrightii Kuntz (Limonium wrightii (Hance) Kuntze), Limonium tenellum Kuntz (Limonium
Tenellum (Turcz.) Kuntze), Limonium otolepis Kuntz (Limonium otolepis (Schrenk) Ktze.) and Huang
One or more in flower Herba Limonii Gmelinii (Limonium aureum (L.) Hill)；More preferably mend for Statice
Blood grass (Limonium sinense (Girard) Kuntze).
3. preparation method as claimed in claim 1, it is characterised in that method one and the step of method two
Suddenly, in (1), described be extracted as supersound extraction, stirring extraction or circulated in countercurrent are extracted, and described is super
The time that sound extracts is preferably 0.5～1.5 hour, and the time that described stirring is extracted is preferably 1.5～2
Hour, the time that described circulated in countercurrent is extracted is preferably 0.3～1 hour.
4. preparation method as claimed in claim 1, it is characterised in that method one and the step of method two
Suddenly in (1), described solvent be percent by volume be the ethanol water of 60～80%；
And/or, described Herba Limonii Gmelinii is 10～40mL/g with the volume mass ratio of solvent；
And/or, described water is deionized water；
And/or, described resin is D101 resin or AB-8 resin.
5. preparation method as claimed in claim 1, it is characterised in that in the step (2) of method two,
Described ethanol solution eluting be successively with 10%～30% ethanol water, 31%～50% ethanol water,
51%～70% ethanol water, 71%～90% ethanol water and 91%～99% ethanol water carry out dense
Degree gradient elution, percentage ratio is percent by volume.
6. preparation method as claimed in claim 5, it is characterised in that in the step (2) of method two,
Described ethanol solution eluting is successively with 20% ethanol water, 40% ethanol water, 60% ethanol
Aqueous solution, 80% ethanol water and 95% ethanol water carry out Concentraton gradient eluting；Percentage ratio is body
Long-pending percentage ratio.
7. the Herba Limonii Gmelinii extract that the preparation method as according to any one of claim 1～6 prepares.
8. Herba Limonii Gmelinii extract as claimed in claim 7, it is characterised in that described Herba Limonii Gmelinii carries
Taking thing is the extract that 31%～50% ethanol water eluting obtains, and percentage ratio is percent by volume；Relatively
Goodly, described Herba Limonii Gmelinii extract is the extract that 40% ethanol water eluting obtains；Percentage ratio is
Percent by volume.
9. Herba Limonii Gmelinii extract as claimed in claim 7 or 8 is as tyrosinase inhibitor, elasticity
Protease inhibitor, antioxidant activity agent or the application of anti-aging actives.
Apply the most as claimed in claim 9, it is characterised in that described Herba Limonii Gmelinii extract is in system
Application in standby skin care item or cosmetics.
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| CN201510158305.XA CN106138122B (en) | 2015-04-03 | 2015-04-03 | Limonium sinense (DC.) kuntze extract and preparation method and application thereof |
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| CN201510158305.XA CN106138122B (en) | 2015-04-03 | 2015-04-03 | Limonium sinense (DC.) kuntze extract and preparation method and application thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021008265A1 (en) * | 2019-07-18 | 2021-01-21 | 枣庄学院 | Anti-aging soft capsule for menopausal women and preparation method therefor |
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| CN102920753A (en) * | 2012-11-05 | 2013-02-13 | 江苏省中国科学院植物研究所 | Dichromatism gmelin sealavender herb extractive and application thereof |
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- 2015-04-03 CN CN201510158305.XA patent/CN106138122B/en not_active Expired - Fee Related
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102920753A (en) * | 2012-11-05 | 2013-02-13 | 江苏省中国科学院植物研究所 | Dichromatism gmelin sealavender herb extractive and application thereof |
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| Title |
|---|
| JI-YOUNG MOON等: "Screening of elastase and tyrosinase inhibitory activity from Jeju Island plants", 《EURASIAN JOURNAL OF BIOSCIENCES》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021008265A1 (en) * | 2019-07-18 | 2021-01-21 | 枣庄学院 | Anti-aging soft capsule for menopausal women and preparation method therefor |
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| CN106138122B (en) | 2020-08-25 |
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