CN102920753A - Dichromatism gmelin sealavender herb extractive and application thereof - Google Patents
Dichromatism gmelin sealavender herb extractive and application thereof Download PDFInfo
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- CN102920753A CN102920753A CN2012104340412A CN201210434041A CN102920753A CN 102920753 A CN102920753 A CN 102920753A CN 2012104340412 A CN2012104340412 A CN 2012104340412A CN 201210434041 A CN201210434041 A CN 201210434041A CN 102920753 A CN102920753 A CN 102920753A
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- herba limoii
- limoii bicoloris
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Abstract
The invention discloses a dichromatism gmelin sealavender herb extractive and application thereof. The dichromatism gmelin sealavender herb serves as a raw material, water and ethanol serve as solvents for extraction, filtering is performed, a water extracting solution or an ethanol extracting solution are obtained, drying is performed to obtain the extractive; and the concentration of the ethanol is 70-95%. The dichromatism gmelin sealavender herb extractive has high Alpha-glucosidase inhibitory activity.
Description
Technical field
The present invention relates to a kind of Herba Limoii Bicoloris extract, be specifically related to a kind ofly have
αThe Herba Limoii Bicoloris extract of-Glucosidase inhibitor activity.
Background technology
Herba Limoii Bicoloris is the herbaceos perennial of Plumbaginaceae Statice, calls Herba Chenopodii Hybridi, CANGYINGHUA, fly grass, burns the eyebrow Artemisia.Be distributed in the ground such as Hebei, Henan, Shanxi, Gansu, Shaanxi.Be born in beach sebkha, the sand dune, meadow of height above sea level 500～2 000 m.All herbal medicine, the sweet little puckery hardship of nature and flavor, nontoxic.Major function: blood yiqi, hemostasis dissipating blood stasis.Cure mainly the diseases such as dysfunctional uterine hemorrhage, cervical cancer, menoxenia, pyelonephritis, gastric ulcer.Herba Limoii Bicoloris enjoys people's concern with its definite medical value and high ecological value, in recent years to the development and use of this medical material also gradually deeply.
α-glucosidase (EC 3.2.1.20) claims again
α-glucoside hydrolytic enzyme, maltase, it can cut from the non-reducing end of oligosaccharides substrate
α-Isosorbide-5-Nitrae glycosidic bond discharges glucose, plays a crucial role in the multiple metabolic function of body.It and many diseases that causes because of metabolism disorder imbalance such as diabetes, cancer, viral infection etc. are closely related.For a long time, people attempt to seek suitable inhibitor, in the hope of diseases related is prevented and treated.
Summary of the invention
To the objective of the invention is the defective that exists in the prior art in order solving, to provide a kind of and have
αThe Herba Limoii Bicoloris extract of-Glucosidase inhibitor activity.
In order to achieve the above object, the invention provides a kind of Herba Limoii Bicoloris extract, take Herba Limoii Bicoloris as raw material, take water or ethanol as solvent extraction, filter, obtain aqueous extract or the ethanol extract drying makes; Wherein concentration of alcohol is 70-95%.
When extracting solvent and be water, Herba Limoii Bicoloris through the ethanol extraction of 70-95%, filters first with before the water extraction, and the Herba Limoii Bicoloris of getting after the filtration extracts with the said extracted aqueous solvent again.
Preferred version is: aqueous extract first through macroporous resin column chromatography, adopts water and ethanol elution successively before dry, get ethanol elution, is drying to obtain the Herba Limoii Bicoloris extract.Wherein ethanol is as eluant, and preferred concentration is 30-50%.
Preferred plan is: above-mentioned ethanol elution first through polyamide column chromatography, adopts water and ethanol elution successively before dry, get ethanol and partly be drying to obtain described Herba Limoii Bicoloris extract.Wherein the type of elution of polyamide column is preferably gradient elution, adopts successively water, 30%, 50%, 70% and 95% ethanol elution, gets respectively each section ethanol elution, drying.
When the extraction solvent is ethanol; The ethanol extract that obtains passes through first petroleum ether extraction before drying, the extracting solution drying after extraction makes the Herba Limoii Bicoloris extract.
Preferred version is: after the ethanol extract drying, and the water suspendible, through petroleum ether extraction, the water drying after the extraction makes described Herba Limoii Bicoloris extract.
Preferred plan is: above-mentioned ethanol extract or water behind petroleum ether extraction also need pass through ethyl acetate extraction before drying, then drying makes above-mentioned Herba Limoii Bicoloris extract.
Its Raw adopts under ground portion or the aerial parts of Herba Limoii Bicoloris.
The present invention also provides above-mentioned Herba Limoii Bicoloris extract in preparation
αThe application of-glucosidase inhibitor aspect.
The present invention has the following advantages compared to existing technology: the Herba Limoii Bicoloris extract of the present invention's preparation is compared the positive control acarbose and is all had higher
α-Glucosidase inhibitor is active.Utilize simultaneously the different position of solvent extraction of opposed polarity, and by macroporous resin and polyamide column chromatography, screen, remove the non-activity part, obtain higher
αThe extract of-Glucosidase inhibitor activity.The Herba Limoii Bicoloris extract of the present invention's preparation can effectively substitute existing
α-glucosidase inhibitor, and the medicine that can be used as diabetes to type Ⅱdiabetes mellitus treatment further investigate.
The specific embodiment
The present invention is described in detail below in conjunction with specific embodiment.
Herba Limoii Bicoloris (
Limonium bicolorKuntze) adopt in Mount Taibai, Mei County, Shaanxi,
α-glucosidase (E.C.232-604-7) is purchased from Sigma company, and (NO. G-5003, Lot. 081K4715), the PNPG substrate is purchased from Amresco, and the microplate reader model is Tecan Infinite F50.
α-Glucosidase inhibitor activity is measured in 96 orifice plates, and 10
μL
α-glucoside enzymatic solution (PBS with 0.067mol/L PH6.8 is mixed with 1U/mL)+20
μThe PBS+2 of L0.067mol/L PH6.8
μL testing sample solution (10mg/ml), behind 37 ℃ of incubation 10min+30
μL PNPG (PBS with 0.067mol/L PH6.8 is mixed with 10mmol/L) measures absorbance at 405nm behind 37 ℃ of incubation 15 min, with acarbose as positive control.Suppression ratio=[(A enzyme-A enzyme is empty)-(A sample-A sample is empty)]/(A enzyme-A enzyme is empty) * 100%
Product embodiments one
Get dry behind the Herba Limoii Bicoloris under ground portion wash clean, pulverize after with 95% ethanol (10 times of amounts) reflux, extract, 2 hours, filter to get filtrate and filtering residue; Get the dry extract Fr.1 of getting of filtrate decompression.
Product embodiments two
After the filtering residue that obtains in the product embodiments one volatilized ethanol, through 10 times of decocting in water 2 hours, after the water extraction liquid drying extract Fr.2.
Product embodiments three
To use petroleum ether extraction behind the extract Fr.1 water suspendible that obtain in the product embodiments one, extraction obtains water and petroleum ether mutually; Get petroleum ether phase drying under reduced pressure, get the extract Fr.1.1 of petroleum ether part.
Product embodiments four
The water that obtains after the extraction in the product embodiments three through ethyl acetate extraction, is obtained water and ethyl acetate mutually after the extraction; Get ethyl acetate phase drying under reduced pressure, get the extract Fr.1.2 of ethyl acetate extract.
Product embodiments five
With the water that behind ethyl acetate extraction, obtains in the product embodiments four, again through n-butanol extraction, obtain water and n-butyl alcohol mutually, drying under reduced pressure gets the extract Fr.1.3 of n-butanol portion.
Product embodiments six
With the water drying under reduced pressure that behind n-butanol extraction, obtains in the product embodiments five, the extract Fr.1.4 at De Shui position.
Product embodiments seven
Get dry behind the Herba Limoii Bicoloris under ground portion wash clean, pulverize after with 70% ethanol (10 times of amounts) reflux, extract, 2 hours, filter to get filtrate and filtering residue; Get the dry extract Fr.3 of getting of filtrate decompression.
Product embodiments eight
After the filtering residue that obtains in the product embodiments seven volatilized ethanol, through 10 times of decocting in water 2 hours, after the water extraction liquid drying extract Fr.4.
Product embodiments nine
To use petroleum ether extraction behind the extract Fr.3 water suspendible that obtain in the product embodiments seven, extraction obtains water and petroleum ether mutually; Get petroleum ether phase drying under reduced pressure, get the extract Fr.3.1 of petroleum ether part.
Product embodiments ten
The water that obtains after the extraction in the product embodiments nine through ethyl acetate extraction, is obtained water and ethyl acetate mutually after the extraction; Get ethyl acetate phase drying under reduced pressure, get the extract Fr.3.2 of ethyl acetate extract.
Product embodiments 11
With the water that behind ethyl acetate extraction, obtains in the product embodiments ten, again through n-butanol extraction, obtain water and n-butyl alcohol mutually, drying under reduced pressure gets the extract Fr.3.3 of n-butanol portion.
Product embodiments 12
With the water drying under reduced pressure that behind n-butanol extraction, obtains in the product embodiments 11, the extract Fr.3.4 at De Shui position.
Product embodiments 13
Get dry behind the Herba Limoii Bicoloris under ground portion wash clean, pulverize after with 80% ethanol (10 times of amounts) reflux, extract, 2 hours, filter to get filtrate and filtering residue; Get the dry extract Fr.5 of getting of filtrate decompression.
Product embodiments 14
After the filtering residue that obtains in the product embodiments 13 volatilized ethanol, through 10 times of decocting in water 2 hours, after the water extraction liquid drying extract Fr.6.
Product embodiments 15
To use petroleum ether extraction behind the extract Fr.5 water suspendible that obtain in the product embodiments 13, extraction obtains water and petroleum ether mutually; Get petroleum ether phase drying under reduced pressure, get the extract Fr.5.1 of petroleum ether part.
Product embodiments 16
The water that obtains after the extraction in the product embodiments 15 through ethyl acetate extraction, is obtained water and ethyl acetate mutually after the extraction; Get ethyl acetate phase drying under reduced pressure, get the extract Fr.5.2 of ethyl acetate extract.
Product embodiments 17
With the water that behind ethyl acetate extraction, obtains in the product embodiments 16, again through n-butanol extraction, obtain water and n-butyl alcohol mutually, drying under reduced pressure gets the extract Fr.5.3 of n-butanol portion.
Product embodiments 18
With the water drying under reduced pressure that behind n-butanol extraction, obtains in the product embodiments 17, the extract Fr.5.4 at De Shui position.
Product embodiments 19
The rear pulverizing (21kg) of drying in the shade of the aerial parts of getting Herba Limoii Bicoloris, with the ethanol merceration of 75L80% one month, filtrate was concentrated, and filtering residue continues with the ethanol merceration of 75L80% one month, and filtrate merges the concentrated extract Fr.7 of getting.
Product embodiments 20
The upper D101 macroporous resin of extract Fr.7 of getting in the product embodiments 19 carries out column chromatography, difference water, 30%, 50%, 70%, 95% ethanol elution, after the split reduction drying, get respectively water elution position a, the extract Fr.7.1 of 30% alcohol elution, extract Fr.7.2, the 70% alcohol elution b of 50% alcohol elution, the extract Fr.7.3 of 95% alcohol elution.
Product embodiments 21
Get polyamide column in the extract Fr.7.1 continuation of macroporous resin 30% alcohol elution in the product embodiments 20, water, 30%, 50%, 70%, 95% ethanol elution get c, Fr.7.1.1, Fr.7.1.2, Fr.7.1.3, Fr.7.1.4 successively after the split reduction drying respectively.
Product embodiments 22
Get polyamide column in the extract Fr.7.2 continuation of macroporous resin 50% alcohol elution in the product embodiments 20, water, 30%, 50%, 70%, 95% ethanol elution get d, Fr.7.2.1, Fr.7.2.2, Fr.7.2.3, Fr.7.2.4 successively after the split reduction drying respectively.
Application Example
Extract Fr.1, the Fr.2, Fr.1.1, Fr.1.2, Fr.1.3, Fr.1.4, Fr.3, Fr.4, Fr.3.1, Fr.3.2, Fr.3.3, Fr.3.4, Fr.5, Fr.6, Fr.5.1, Fr.5.2, Fr.5.3, Fr.5.4, Fr.7, Fr.7.1, Fr.7.3, Fr.7.2, Fr.7.1.1, Fr.7.1.2, Fr.7.1.3, Fr.7.1.4, Fr.7.2.1, Fr.7.2.2, Fr.7.2.3 and the Fr.7.2.4 that get product embodiments preparation of the present invention carry out
αThe determination of activity of-Glucosidase inhibitor, the result is as shown in the table.
Each extract of table 1
α-Glucosidase inhibitor determination of activity result
As can be seen from the above table, the extract of the present invention preparation is compared the positive control acarbose and is had higher
α-Glucosidase inhibitor is active.Wherein with extract Fr.3.3(70% alcohol reflux aerial parts, n-butanol portion), extract Fr.7.1.3(75% ethanol merceration extracts under ground portion, polyamide 70% ethanol elution part behind macroporous resin 30% ethanol elution) and extract Fr.7.2.1(75% ethanol merceration extract under ground portion, kymene 0% ethanol elution part behind macroporous resin 50% ethanol elution)
α-Glucosidase inhibitor is active best.
Claims (10)
1. Herba Limoii Bicoloris extract is characterized in that: described Herba Limoii Bicoloris extract is take Herba Limoii Bicoloris as raw material, take water or ethanol as solvent extraction, filters, and obtains aqueous extract or the ethanol extract drying makes; Described concentration of alcohol is 70-95%.
2. Herba Limoii Bicoloris extract according to claim 1, it is characterized in that: described extraction solvent is water; Described Herba Limoii Bicoloris through the ethanol extraction of 70-95%, filters first with before the water extraction, and the Herba Limoii Bicoloris of getting after the filtration extracts with described extraction aqueous solvent.
3. Herba Limoii Bicoloris extract according to claim 1 is characterized in that: described aqueous extract is first before dry through water and ethanol elution, gets ethanol elution successively through macroporous resin column chromatography, is drying to obtain described Herba Limoii Bicoloris extract; The concentration of described eluent ethanol is 30-50%.
4. Herba Limoii Bicoloris extract according to claim 3 is characterized in that: described ethanol elution is first before dry to adopt water and ethanol elution successively through polyamide column chromatography, gets ethanol and partly is drying to obtain described Herba Limoii Bicoloris extract.
5. Herba Limoii Bicoloris extract according to claim 1, it is characterized in that: described extraction solvent is ethanol; Described ethanol extract passes through first petroleum ether extraction before drying, the extracting solution drying after extraction makes described Herba Limoii Bicoloris extract.
6. Herba Limoii Bicoloris extract according to claim 5 is characterized in that: after the described ethanol extract drying, and the water suspendible, through petroleum ether extraction, the water drying after the extraction makes described Herba Limoii Bicoloris extract.
7. Herba Limoii Bicoloris extract according to claim 5, it is characterized in that: described ethanol extract behind petroleum ether extraction also need pass through ethyl acetate extraction before drying, then drying makes described Herba Limoii Bicoloris extract.
8. Herba Limoii Bicoloris extract according to claim 6, it is characterized in that: described water behind petroleum ether extraction also need pass through ethyl acetate extraction before drying, then drying makes described Herba Limoii Bicoloris extract.
9. Herba Limoii Bicoloris extract according to claim 1 is characterized in that: under ground portion or the aerial parts of described raw material employing Herba Limoii Bicoloris.
10. the arbitrary described Herba Limoii Bicoloris extract of claim 1 to 9 is in preparation
αThe application of-glucosidase inhibitor aspect.
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| CN106038624A (en) * | 2016-06-01 | 2016-10-26 | 鲁东大学 | Limonium franchetii antineoplastic active constituent extract and preparation method and application thereof |
| CN106137811A (en) * | 2015-04-03 | 2016-11-23 | 伽蓝（集团）股份有限公司 | A kind of composition of plant extracts and application thereof |
| CN106138122A (en) * | 2015-04-03 | 2016-11-23 | 伽蓝（集团）股份有限公司 | Herba Limonii Gmelinii extract and its production and use |
| CN110548071A (en) * | 2019-09-29 | 2019-12-10 | 四川梓橦宫药业股份有限公司 | application of paliurus ramosissimus or extract thereof in preparation of anti-tumor metastasis medicaments |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106137811A (en) * | 2015-04-03 | 2016-11-23 | 伽蓝（集团）股份有限公司 | A kind of composition of plant extracts and application thereof |
| CN106138122A (en) * | 2015-04-03 | 2016-11-23 | 伽蓝（集团）股份有限公司 | Herba Limonii Gmelinii extract and its production and use |
| CN106138122B (en) * | 2015-04-03 | 2020-08-25 | 伽蓝（集团）股份有限公司 | Limonium sinense (DC.) kuntze extract and preparation method and application thereof |
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| CN105362314B (en) * | 2015-12-09 | 2019-10-29 | 江苏省中国科学院植物研究所 | A kind of new application of twocolor sealavander herb ethyl acetate extract |
| CN106038624A (en) * | 2016-06-01 | 2016-10-26 | 鲁东大学 | Limonium franchetii antineoplastic active constituent extract and preparation method and application thereof |
| CN106038624B (en) * | 2016-06-01 | 2019-11-29 | 鲁东大学 | Yantai herba limonii gmelinii anti-cancer effective component extract and its preparation method and application |
| CN110548071A (en) * | 2019-09-29 | 2019-12-10 | 四川梓橦宫药业股份有限公司 | application of paliurus ramosissimus or extract thereof in preparation of anti-tumor metastasis medicaments |
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Application publication date: 20130213 |