CN101732370A - Method for extracting an alpha-glucosidase active inhibitor from Limonium gmelinii(Willd.)Kuntze - Google Patents
Method for extracting an alpha-glucosidase active inhibitor from Limonium gmelinii(Willd.)Kuntze Download PDFInfo
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- CN101732370A CN101732370A CN200910249049A CN200910249049A CN101732370A CN 101732370 A CN101732370 A CN 101732370A CN 200910249049 A CN200910249049 A CN 200910249049A CN 200910249049 A CN200910249049 A CN 200910249049A CN 101732370 A CN101732370 A CN 101732370A
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Abstract
The invention discloses a method for extracting an alpha-glucosidase active inhibitor from Limonium gmelinii(Willd.)Kuntze that the Limonium gmelinii (Willd.)Kuntze is used as raw material; the product has the advantages of no toxic or side effect, high safety, low cost and industrial production. The method comprises the following steps of: cutting and crushing the Limonium gmelinii(Willd.)Kuntze, and putting into an extractor; adding an alcohol-water solution or an acetone-water solution, wherein the ratio of the volume of the alcohol-water solution or the acetone-water solution to mass ratio of the Limonium gmelinii(Willd.)Kuntze is 8-30 ml/g, and extracting by heating and refluxing, wherein the extracting temperature reaches to boiling temperature, the extracting is conducted once to three times, and each extracting lasts 1.0-3.0 h; collecting, and combining the cooled extracting solution, depressurizing to concentrate, freezing and drying to obtain the Limonium gmelinii(Willd.)Kuntze extract.
Description
Technical field:
The present invention relates to a kind of extracting method of alpha-glucosidase activity inhibitor, especially a kind of is raw material with the Limonium gmelinii (Wildl.) Kuntze herb, but the method for from Limonium gmelinii (Wildl.) Kuntze, extracting alpha-glucosidase activity inhibitor that product has no side effect, the cheap industrialization of safe, cost is produced.
Background technology:
Diabetes (Diabetes mellitus, DM) be a kind of metabolic disease group of multi-pathogenesis, mainly produce relevant unusually with effect with insulin, can cause sugar, fat, protein metabolism disorder, with blood glucose increase with glucose in urine be feature, and then cause the syndrome of a plurality of systems, a plurality of organ injuries, be the human main cause of suffering from cardiovascular and cerebrovascular disease, blinding, amputation and renal failure.Having developed multiple novel blood sugar lowing chemicals at present is applied to clinical, as improve glucose tolerance and insulin replies level insulin secretion stimulators (dipeptidyl peptidase enzyme DPP-IV inhibitor), improve glucose metabolism agent (biguanides), can effectively improve the metabolism of body polyalcohols, prevent and delay aldose reductase inhibitor (Pyrrolizidine lopps material) and the euglycemic agent that disease takes place.Wherein euglycemic agent has become the research and development focus of present orally-taken blood sugar reducing medicine, can increase the sensitivity of tissue to insulin, alpha-glucosidase inhibitor particularly, as acarbose, its mechanism of action is for suppressing alpha-glucosidase competitively, final step inhibition disaccharidase at carbohydrate digestion is degraded to monosaccharide, delay the digestion of carbohydrate at intestinal, delay is from disaccharidase, the glucose absorption of oligosaccharide and polysaccharide, effectively postponing and alleviate time and the process that after-dinner blood sugar of diabetes patients raises, is the medicine of the blood sugar control used always of diabetes patient.
Though existing anti-diabetic kind is more, can determined curative effect is effective blood sugar control, but be chemicals, it is single to exist action pathway, even has serious adverse effects, as cause hypoglycemia, lactic acidosis etc., take the possibility that reduces with drug effect for a long time.
Limonium gmelinii (Wildl.) Kuntze Limonium gmelinii (Wildl.) Kuntz is the Plumbaginaceae of Bai Danhua section, and Herba Limonii Gmelinii Limonium Mill. belongs to herbaceos perennial.The Statice plant contains the number of chemical composition, as aminoacid, vitamin, flavonoid, tannin, polysaccharide, alkaloid, organic acid etc., have enrich blood, hemostasis, anti-inflammation, loose silt, regulating menstruation, strengthening the spleen, multiple efficacies such as be good for the stomach, be mainly used in treatment cervical cancer and dysfunctional uterine hemorrhage disease at present clinically, up to now also not about from Limonium gmelinii (Wildl.) Kuntze, extracting the alpha-glucosidase activity inhibitor relevant report.
Summary of the invention:
The present invention is in order to solve the above-mentioned technical problem of existing in prior technology, providing a kind of is raw material with the Limonium gmelinii (Wildl.) Kuntze herb, but the method for from Limonium gmelinii (Wildl.) Kuntze, extracting alpha-glucosidase activity inhibitor that product has no side effect, the cheap industrialization of safe, cost is produced.
Technical solution of the present invention is: a kind of method of extracting alpha-glucosidase activity inhibitor from Limonium gmelinii (Wildl.) Kuntze is characterized in that carrying out as follows:
A. get the Limonium gmelinii (Wildl.) Kuntze herb, put into extractor after shredding grinding, adding volume and Limonium gmelinii (Wildl.) Kuntze herb mass ratio is alcohol-water or the acetone-water solution soaking of 8~30ml/g, heating and refluxing extraction, extract temperature for reaching to boiling temperature, extraction time is 1~3 time, and each extraction time is 1.0~3.0h;
B. collect the cooled extracting solution of merging, concentrating under reduced pressure, lyophilization gets the Limonium gmelinii (Wildl.) Kuntze extract.
The determining alcohol of the alcohol-water solution of described a step is 70%~100%, and alcohol wherein is methanol, ethanol or propanol, the acetone concentration 50%~100% in the described acetone-water solution.
The present invention is to be raw material with wide material sources, cheap Limonium gmelinii (Wildl.) Kuntze, utilize solvent extraction method that the Limonium gmelinii (Wildl.) Kuntze herb is extracted, resulting extract suppresses experimental results show that through alpha-glucosidase activity, have the effect of significant inhibition alpha-glucosidase activity and have no side effect, safe, cost is cheap, can be widely used in preparing in the medicine and health product that reduces diabetes.The present invention is simple to operate, and solvent for use is general chemistry reagent, easily repeats, and is suitable for large-scale production.
Description of drawings:
Fig. 1 is the embodiment of the invention 3 extract concentrations and the active corresponding curve chart of the inhibition of alpha-glucosidase.
The specific embodiment:
Embodiment 1:
A. accurately take by weighing Limonium gmelinii (Wildl.) Kuntze herb 10g, shred to grind in the acetone-water solution that immerses 100ml 100% in the back and spend the night, adopt the apparatus,Soxhlet's heating and refluxing extraction, extract temperature for reaching to boiling temperature, extraction time is 2 times, and each extraction time is 2h;
B. collect the cooled extracting solution of merging, rotate evaporation and concentration down at 50 ℃, lyophilization gets the Limonium gmelinii (Wildl.) Kuntze extract.
Get the embodiment of the invention 1 extract, the adding distil water standardize solution, compound concentration is the Limonium gmelinii (Wildl.) Kuntze extract solution of 3.125mg/mL, carry out alpha-glucosidase according to art methods and suppress activity experiment, recording suppression ratio is 76.04%, apparently higher than positive control--and acarbose (suppression ratio is 39.5% under equal concentrations).
Embodiment 2:
A. accurately take by weighing Limonium gmelinii (Wildl.) Kuntze herb 10g, shred to grind in the acetone-water solution that immerses 300ml 50% in the back and spend the night, adopt the apparatus,Soxhlet's heating and refluxing extraction, extract temperature for reaching to boiling temperature, extraction time is 3 times, and each extraction time is 1h;
B. collect the cooled extracting solution of merging, rotary evaporation concentrates, and lyophilization gets the Limonium gmelinii (Wildl.) Kuntze extract.
Get the embodiment of the invention 2 extracts, the adding distil water standardize solution, compound concentration is the Limonium gmelinii (Wildl.) Kuntze herb extract solution of 1.563mg/mL, carry out alpha-glucosidase and suppress activity experiment, recording suppression ratio is 56.3%, with concentration be the positive control of 6.25mg/mL--the suppression ratio (51.3%) of acarbose is suitable.
Embodiment 3:
A. accurately take by weighing Limonium gmelinii (Wildl.) Kuntze herb 10g, shred to grind in ethanol (methanol or the propanol) solution that immerses 100ml 70% in the back and spend the night, adopt the apparatus,Soxhlet's heating and refluxing extraction, extract temperature for reaching to boiling temperature, extraction time is 1 time, and each extraction time is 3h;
B. collect cooled extracting solution, rotate evaporation and concentration down at 50 ℃, lyophilization gets the Limonium gmelinii (Wildl.) Kuntze extract.
Get the embodiment of the invention 3 extracts, the adding distil water standardize solution, dilute successively that compound concentration is respectively 2,1,0.5, the Limonium gmelinii (Wildl.) Kuntze herb extract solution of 0.25mg/mL, carry out alpha-glucosidase according to the method for prior art and suppress activity experiment, record four extracts under the concentration alpha-glucosidase is all had the activity of inhibition, its activity exists dose-dependence (see figure 1), IC
50Value is the 1.77mg/mL (IC of positive control drug acarbose
50Value is 6.25mg/mL).
The extract that the embodiment of the invention 1~3 prepares can be used as treatment and prevent diabetes and complication medicine thereof various preparations, suppress the health food of diabetes and complication thereof and as the food additive of blood sugar lowering etc.
The medicine of treatment and prevent diabetes and complication thereof or health food can mix forming various forms of pills, powder, tablet, unguentum, powder, injection, water preparation, granule, capsule, injection etc. with adjuvant; The health food form can be liquid such as the picture refreshment drink, soda pop, flavoring agent; As additive, such as joining the wheaten food class, fruit jelly, ice cream, curing food, Petaso, point is in the heart.
Claims (2)
1. method of extracting alpha-glucosidase activity inhibitor from Limonium gmelinii (Wildl.) Kuntze is characterized in that carrying out as follows:
A. get the Limonium gmelinii (Wildl.) Kuntze herb, put into extractor after shredding grinding, adding volume and Limonium gmelinii (Wildl.) Kuntze herb mass ratio is alcohol-water or the acetone-water solution soaking of 8~30ml/g, heating and refluxing extraction, extract temperature for reaching to boiling temperature, extraction time is 1~3 time, and each extraction time is 1.0~3.0h;
B. collect the cooled extracting solution of merging, concentrating under reduced pressure, lyophilization gets the Limonium gmelinii (Wildl.) Kuntze extract.
2. the method for from Limonium gmelinii (Wildl.) Kuntze, extracting alpha-glucosidase activity inhibitor according to claim 1, it is characterized in that: the determining alcohol of the alcohol-water solution of described a step is 70%~100%, alcohol wherein is methanol, ethanol or propanol, the acetone concentration 50%~100% in the described acetone-water solution.
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN102920753A (en) * | 2012-11-05 | 2013-02-13 | 江苏省中国科学院植物研究所 | Dichromatism gmelin sealavender herb extractive and application thereof |
CN106666475A (en) * | 2016-12-15 | 2017-05-17 | 南陵县玉竹协会 | Mustard-flavor healthcare salmon can |
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JP4903751B2 (en) * | 2008-05-21 | 2012-03-28 | 株式会社琉球バイオリソース開発 | Antioxidant |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102920753A (en) * | 2012-11-05 | 2013-02-13 | 江苏省中国科学院植物研究所 | Dichromatism gmelin sealavender herb extractive and application thereof |
CN106666475A (en) * | 2016-12-15 | 2017-05-17 | 南陵县玉竹协会 | Mustard-flavor healthcare salmon can |
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