KR100473531B1 - Composition containing an extract of truncated sopungsungi-won crude drug complex for preventing and treating diabetes - Google Patents

Abstract

The present invention relates to a composition for preventing and treating diabetes comprising an extract consisting of a peritropic excipient physiological herbal compound, that is, rhubarb, charcoal, earth and sand, fruit room, windproof, and poisonous activity, the composition of the present invention inhibits weight gain and lowers blood sugar. It has a therapeutic effect such as lowering of triglyceride and free fatty acid in blood, so it can be used as a medicine and health supplement food useful for the prevention and treatment of diabetes and various complications.

Description

COMPOSITION CONTAINING AN EXTRACT OF TRUNCATED SOPUNGSUNGI-WON CRUDE DRUG COMPLEX FOR PREVENTING AND TREATING DIABETES}

The present invention relates to a composition for the prevention and treatment of diabetes, including the extract of herbal excipient sedative raw herbs extract.

Type 2 diabetes, also known as insulin-independent diabetes, is a disease that occurs in middle-aged adults, and in Western society, about 2 to 6% of adults suffer from this disease. Insufficiency of insulin secretion due to insulin resistance and disrupted pancreas leads to metabolic diseases causing hyperglycemia. It is reported that such diabetes can inhibit the onset and progression of diabetic disease through thorough urine glucose control and control (Diabetes Controlled Clinical Research Association, N. Eng. J. Med., 329 , p977-986, 1993). Hyperglycemia can also be caused by various pathologies such as hyperosmolarity, oxidant formation, abnormal metabolism of sorbitol and myoinositol, and activation of diacylglycerol (DAG) -protein kinase C (PKC). It has been reported in Brownlee et al . (Brownlee et al: J. Engl. J. Med. 318 , p1315-1321, 1998), and diabetic nephropathy is a late stage. Renal disease (ESRF) is a typical etiology and the early stages of diabetic nephropathy are characterized by glomerular epilepsy, accumulation of extracellular matrix, and increased urinary albumin excretion. Obvious nephropathy clinically causes proteinuria, hypertension and progressive renal failure.

High levels of blood glucose pose a risk to nonenzymatic and enzymatic mechanisms, including major signal transduction processes and regulation of hypertrophic and prefibrous cytokine transforming growth factors (TGF-β), according to Sharma et al. Sharma K and Ziyadeh FN: Semin.Neprol. 17 , p 80-92, 1997). Concerning the cellular signaling process, high concentrations of glucose increase DAG neosynthesis with PKC activation, according to Craven PA and De Rubertis FR: J. Cln. Invest. 83 , p1667. -1675, 1989), the activation of PKC is an important intracellular mediator of diabetic complications, and the use of nonspecific and specific inhibitors for PKC isozyme has been shown to result in high concentrations in in vitro and in vivo tests of diabetic kidney disease. Inhibiting the transformation of glucose-induced cellular function has been reported in Studer et al. (Studer RK, et al: Diabetes 42 , p118-126, 1993).

More recently, extracellular signal-regulated kinase-1 / 2 (ERK 1/2 or p42 / 44 MAPK), stress-activated C-Jun N-amino terminal kinase (JNK / Stress-activated protein kinase or SAPK) and p38 Three groups of mitogen-activated protein kinases (MAPKs), such as MAPK, have been identified and isolated, and these enzymes can be activated by a variety of external stimuli, including growth factors and environmental stresses. It has been reported in Segar et al. (Segar R and Krebs EG: FASEB J. Jun, 9 (9) , p726-35, 1995), which plays a key role in the signaling system that drives transformation.

In relation to diabetes, ERK is known to be activated not only in glomerular stromal cells cultured in hyperglycemic state but also in glomeruli of diabetic rats, and ERK activation in these cells is generated through PKC-dependent mechanisms (Haneda et al. Haneda M et al: Diabetes 46 (5) p847-53, 1997), Igarashi et al. , Igarashi M et al. , Found that P38 MAPK is activated by relatively high blood sugar levels in various cells, including glomerular interstitial cells. et al: J. Clin. Invest. 103 (2) p185-95, 1999). In contrast to these two kinases, JNK is not activated in the glomeruli of diabetic rats, Since it is not stimulated, the ERK activation process may be involved in intracellular signaling systems that cause cellular dysfunction in diabetes mellitus, for example ERK is a three-dimensional complex One of them is Janknecht, R et al: EMBO J , which activates Elk-1 and induces c-fos transcription to induce subsequent production of activator protein-1 (AP-1) transfer factor complexes. . Dec (15), it has been reported in 12 (13), p5097-104, 1993 ).

In order to examine the mechanism of drug efficacy, the pancreas, insulin-dependent tissues (muscles, fat, liver, etc.), small intestine, kidneys, etc. were extracted from the experimental animals to check the targets of the cause of diabetes one by one, and then insulin secretion and glucose into the tissues. The effects on influx, carbohydrate metabolism or uptake can be identified using biochemical and molecular biological techniques at the tissue or cellular level.

Various types of conventional diabetes treatments have a function of inhibiting hyperglycemia to some extent, but many patients with type 2 diabetes have difficulty controlling blood sugar and are known to progress to serious complications. Common uses for the treatment of diabetes include oral hypoglycemic agents and insulin administration.

The present invention seeks to find a natural herbal complex composition effective for treating diabetes and the complications as described above.

Excursion Soon Ki-won (疏風 順 氣 元) is stated in Huh Jun's consent and use as a laxative to soften the stool, mainly in the elderly (see agreement, Huh Jun, stool in the inner diameter), the composition of the prescription is rhubarb, Document of the secondary electron, Wook Reinforced, betel, etc. as soon as the, tosaja, wooseul, Yam, Cornus officinalis, jisil, wind, consists of 12 herbal of Aralia cordata, jeongboseop (jeongboseop, sinmingyo low, hyangyak Dictionary, Younglim Inc., 1998 According to)

Rhubarb refers to the roots of Rheum undulatum L., R. officinale B., R. coreanum Nakai, and R. palmatum L. , Antibacterial effect, anti-tumor effect, etc., and its components are known as anthraquinone derivatives such as emodin (emodin), aloe emodin (aloeemodin) (p327-328),

Chason refers to seeds of Plantago asiatica D., P. camtschatica C., P. depressa W. and P. japonica F. It is known to have a therapeutic effect on diuresis, arthritis, etc., and its components are known as aucubin, plantaginin, and ursolic acid (p1004-1006). ,

As a seed of Prunus japonica Thunb var Nakai, P. triloba var truncata Kom, P. tomentosa Thunb, and P. ishidoyana Nakai , Constipation treatment, and its components are known components such as amygdalin (amygdalin), fatty oil, oleic acid (p627-628),

Betel nut is a seed of betel nut ( Areca catechu L.), and its insecticidal effect is known, and its ingredients are known as alkaloids such as arecoline, guvacine and arecolidine. ,

Mazarin (麻子 仁) is a seed of hemp ( Cannabis sativa L.), and it is known to treat constipation, and its components include fatty oil, oleic acid, and linolenic acid (p548-550).

Earthworms are the seeds of Cuscuta japonica Choisy, C. australis R. and C. chinensis Lam, which are known as potents and tonics, and include resin glycosides and vitamin A. Components are known (p882-883),

Root is the root of Achyranthus japonica Nakai and A. bidentata Bl. It is known to have diuretic and analgesic effects. Its components include ecdysone and ecdys Components such as ecdysterone and triterpenoid saponin are known (p340-341),

Sanya is a tuber of hemp ( Dioscorea batatas Decne) and Yam ( D. japonica Thunb) and is known as a tonic and tonic tablet. Abscisin) are known (p147-149),

Cornus of the Cornus officinalis Sieb is used as a tonic and tonic, and its constituents include cornin, tannin and vitamin A (p448-449).

Fruits are young fruits of Citrus aurantium L., and are known for their effects on gastrointestinal diseases and uterine contractions. Flavonoid glycosides and vitamin C are known as ingredients (p779-). 780),

Windproof (防風) is the root of Windproof ( Ledebouriella seseloides Wolf.) And Root Sprouts ( Pusedanum japonicum Thunb), known for its antipyretic and analgesic effects. (p428-429),

Toxic activity uses the root and muscle of Aralia cordata Thunb, and is known to be effective in sweating, fever, diuresis, and pain, and its components include limonene and sabinene. Essential oil components such as these are known (p435-436).

However, there have been no reports of the therapeutic effect of diabetes mellitus, which is a combination of the herbal medicines, until now.

The inventors of the present invention, namely, rhubarb, charcoal, ukriin, betel, mazain, earthenware, hyssop, pesticides, cornus, larvae, windproof, poisonous nutrients, The extract of the complex herbal medicine consisting of rhubarb, tea, earth and sand, fruit room, windproof, poisonous extract extract was found to have an excellent therapeutic effect on diabetes and its complications and completed the present invention.

It is an object of the present invention to provide a pharmaceutical composition and dietary supplement showing the effect of preventing and treating diabetes and its complications.

In accordance with the above object, the present invention provides a composition for the prevention and treatment of diabetes mellitus and its complications, including the excursion excursion net origin, ie rhubarb, chajeonja, earth and sand, fruit room, windproof, poisonous extract.

The composition for treating diabetes of the present invention comprises 0.5-50% by weight of the excipient excipient net origin extract based on the total weight of the composition.

Each herbal medicine of the present invention includes all the herbal medicines derived from the same plant, and the composition ratio of the individual compositions is not limited in the present invention, but preferably, two herbal medicines of rhubarb and tea, earthenware, fruit, windproof, The weight ratio of the three medicinal herbs of poisonous activity is about 1 to 10: 1 to 5: 1, more preferably about 3 to 7: 2 to 4: 1, still more preferably about 5: 2.5: 1.

The combined herbal extracts of the present invention are all dried and then crushed in the case of seeds, chopped in the case of root or rhizome, and then water of about 1 to 15 times, preferably about 5 to 10 times the volume of the herbal medicine, Lower alcohol or a mixed solvent having a mixing ratio of about 1: 0.1 to 1:10, preferably 1: 1 to 1: 3, at a extraction temperature of 20 to 100 ° C, preferably 70 to 100 ° C, for about 1 hour to 2 days, preferably about 2 hours to 1 day can be obtained by the extraction method, such as ultrasonic extraction, reflux extraction.

The present invention provides a composition for the prevention and treatment of diabetes and its complications, including water extracts obtained in the extraction process, lower alcohol extracts, and soluble extracts according to a mixed solvent thereof.

The composition comprising the complex herbal extract of the present invention may further comprise a suitable carrier, excipient and diluent according to conventional methods.

Carriers, excipients and diluents that may be included in the composition comprising the complex herbal extract of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin , Calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil.

The composition comprising the herbal extract extract according to the present invention is in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols and the like, oral preparations, suppositories, and sterile injectable solutions, respectively, according to a conventional method. Can be formulated and used.

The amount of the combined herbal extract of the present invention may vary depending on the age, sex, and weight of the patient, but may be administered once to several times daily in an amount of 0.1 to 500 mg / kg. In addition, the dosage of the extract can be increased or decreased depending on the route of administration, the degree of disease, sex, weight, age and the like. Therefore, the above dosage does not limit the scope of the present invention in any aspect.

The composition comprising the complex herbal extract of the present invention may be used in various forms such as pharmaceuticals, foods and beverages for the prevention and treatment of diabetes and its complications. Examples of the food to which the herbal extract can be added include various foods, beverages, gums, teas, vitamin complexes, and health supplements.

The herbal extracts of the present invention are medicaments that can be used with confidence even when taken for long periods of time, as they have little toxicity and side effects.

The complex herbal extract of the present invention may be added to food or beverage for the purpose of preventing diabetes and its complications. At this time, the amount of the complex herbal extract in the food or beverage is generally added to the health food composition of the present invention 0.1 to 15% by weight, preferably 1 to 10% by weight of the total food weight, the food health beverage composition is It can be added in the ratio of 1-30 g, Preferably it is 3-10 g based on 100m <1>.

The health beverage composition of the present invention has no special limitations in the liquid component except for containing the complex herbal extract as an essential ingredient in the indicated ratio, and may contain various flavors or natural carbohydrates as additional ingredients, such as ordinary drinks. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And conventional sugars such as polysaccharides such as dextrin, cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (tauumatin, stevia extract (e.g., rebaudioside A, glycyrrhizin, etc.), and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The ratio of said natural carbohydrate is generally about 1-20g, preferably about 5-12g per 100m1 of the composition of the present invention.

In addition to the above, the composition of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, coloring and neutralizing agents (such as cheese and chocolate), pectic acid and salts thereof, alginic acid and salts thereof. , Organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. The compositions of the present invention may also contain pulp for the production of natural fruit juices and fruit juice beverages and vegetable beverages. These components can be used independently or in combination. The proportion of such additives is not so critical but is generally selected from the range of 0 to about 20 parts by weight per 100 parts by weight of the composition of the present invention.

As a result of the preliminary experiments, the composition of the present invention has been observed that the excursions of the excursions are excellent in hypoglycemic action and triglyceride reduction effect. Based on this, 9 weeks of dry powder administration of extracts of medicinal herbs from the excursion excursions using type 2 diabetic animal model (C57BL / 6J ob / ob mice) resulted in dose-dependent hypoglycemic activity and triglyceride (triglyceride). ) And cholesterol reducing activity was excellent.

The present invention is described in more detail based on the following examples, although the present invention is not limited thereto.

Example 1 Preparation of Extract of Peripheral Picnic Soon Ki Won Complex Herbal Medicine

107.5 volumes of dried or shredded rhubarb rhubarb purchased from the reserve building in Gyeongdong market, tea and pottery 375g each, ground, windproof, and poisonous each 187.5g were added, and 2 at a temperature of 80-100 ° C. The extract obtained by heating under reflux for a time was lyophilized in a freeze-dryer (FN-5N, EYELA, Japan) to use 100 g of the dried excipient net source compound herbal dried product obtained as a sample.

Reference Example 1. Analysis and Statistical Processing

Blood Index Analysis Method

 Blood glucose levels were measured weekly using a Tinder solution (Sigma Diagnostics, USA) using the glucose oxidase method. Blood glycated hemoglobin concentration was measured using glycated hemoglobin HbA1 kit (Sigma) by cation exchange method in blood collected from mice bred for 9 weeks. Serum insulin concentrations and C-peptides were measured using kits by insulin radioimmunoassay and competitive RIA assays prior to administration and in blood at 8 weeks of administration. Blood triglyceride levels were measured using the GPO-Trinder kit (Sigma) and free fatty acid concentrations were measured using the NFAZYME-S kit using the acyl-CoA oxidase method. Blood cholesterol was measured using Sigma's Cholesterol Kit.

Statistical processing method

All experimental results were expressed as mean ± SE, and statistical processing was performed by the Student t-test to determine the significance based on P <0.05.

Experimental Example 1: Weight change and hematuria index change experiment

This experiment was adapted for 5 weeks of diabetic mouse C57BL / 6J ob / ob (Jackson Lab. USA) mice and their normal mouse species C57BL / 6J for 1 week, under constant temperature (25 ± 2 ℃) and constant humidity (50%). Each cage was fed with water and feed (Samyang) and used for the experiment. Six-week-old mice were divided into six groups, the sample-free group and the unilateral excipient net origin treatment group: the untreated group was supplied with powdered general feed for mice (Samyang, South Korea), and the lateral excursion physiological administration group per kg body weight of the mouse. The dry extract of Example 1 was mixed in the feed and reared for 9 weeks to consume 200 mg.

Changes in body weight were measured weekly, and blood obtained by orbital blood collection with heparinized capillaries was centrifuged to obtain plasma, and glucose, triglyceride, and free fatty acid concentrations were measured, and blood collection was always performed at 3 pm. It was. In the blood obtained at the last week of administration, the concentrations of glycated hemoglobin, insulin, C-peptide, cholesterol, and HDL were measured to obtain the following experimental results.

Diet and Water Intake and Weight Changes

In the case of dietary intake, as shown in Table 1, the untreated group increased, but the sample treated group showed a reduction effect of 23% compared to the untreated group, and * P was * P <0.01 for the untreated group.

Characteristic No treatment group Treatment group  Feed (g / per mouse / day) 4.7 ± 0.2 3.6 ± 0.1 *  Water (ml / per mouse / day) 7.9 ± 0.2 4.9 ± 0.2 *

In weight change, as shown in FIG. 1, there was a significant weight loss of 20.0 g in the untreated group and 14.6 g in the sample treated group for 9 weeks, which showed a significant weight loss in the sample treated group consistent with the dietary intake.

Blood glucose and glycated hemoglobin change results

Figure 2 shows the results of measuring the change in blood glucose of the sample untreated group (○), treated group (●) once a week, the sample untreated group fluctuated between 245 and 280 mg / dl during the experimental period However, the sample treated group showed a significant decrease in blood glucose levels from 2 weeks after the treatment compared to the untreated group. At the end of the treatment, the sample treated group showed a 38% reduction in blood glucose compared to the untreated group.

Figure 3 is a result of comparing the blood glycated hemoglobin (HbA _1c ) between groups at the end of the experiment. The mean value of glycated hemoglobin was 4.66% and 3.89% in the untreated group and the sample treated group, respectively. The change in glucose and glycated hemoglobin level in the blood was confirmed that the excursions from the excursion improved hyperglycemia.

Consequences of Changes in Insulin and C-peptides in Blood

Blood insulin and C-peptide were measured at the end of the experiment. As shown in FIG. 4, plasma insulin levels of the untreated and sample-treated groups were 75.6 μU / ml and 16.3 μU / ml, respectively. Experimental results showed a reduction effect of plasma insulin in%.

As with the change in the blood insulin level, the plasma C-peptide values of the untreated group and the sample treated group were 0.71 ng / ml and 0.24 ng / ml, respectively, as shown in FIG. 5. This is a result showing a significant decrease in the concentration of C-peptide in the sample treatment group.

The above experimental data is an experimental result confirming that the excursion excursions in ob / ob mice improves hyperinsulinemia.

Results of lipid change between groups

Table 2 shows the results of experiments on the effects of triglycerides, free fatty acids and cholesterol in blood of the untreated and sample-treated groups. At the end of sample treatment, the sample-treated group had about 24% more blood than the untreated group. Triglyceride levels were decreased, and the concentration of free fatty acids in blood was significantly lower in the sample treated group (1.63 ± 0.06 mEq / L) than in the untreated group (1.96 ± 0.07 mEq / L). . Compared with the untreated group, P values were indicated by * below 0.05, below 0.01, and below 0.001 ***.

Characteristic No treatment group Treatment group   Total cholesterol (mg / dl) 257.5 ± 8.3 176.1 ± 6.3 ^***   HDL (% of total cholesterol) 47.7 ± 1.5 53.2 ± 1.5 ^*   LDL (% of total cholesterol) 44.2 ± 1.9 37.8 ± 1.9 ^* Triglycerides (mg / dl) 104.9 ± 5.3 80.0 ± 4.2 ^**   Free fatty acid (mEq / L) 1.96 ± 0.07 1.63 ± 0.06 ^**

As shown in FIG. 6, the total cholesterol levels in blood of the no treatment group (○) and the sample treatment group (●) at the end of the experiment were 257.5 ± 8.3 mg / dL and 176.1 ± 6.3 mg / dL, respectively. About 32% of the blood cholesterol was reduced.

On the other hand, the sample treatment group was found to have an effect of increasing the blood HDL concentration and reducing the blood LDL level.

Hereinafter, the preparation examples of the pharmaceutical composition will be described, but the present invention is not intended to be limited thereto, but is intended to be described in detail.

Formulation Example 1 Preparation of Powder

According to the preparation method of powder in the pharmacopeia formulation, it is prepared in the following ingredient content per one packet.

Example 1 Dried Powder 300 mg

Lactose · ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ 100 mg

Talc 10 mg

Formulation Example 2 Preparation of Tablet

According to the preparation method of tablets in the pharmacopeia formulation, it is prepared in the following component content per tablet.

Example 1 Dry Powders 300 mg

Corn starch ㆍ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ 100 mg

Lactose · ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ 100 mg

Magnesium Stearate 2 mg

Formulation Example 3 Preparation of Capsule

According to the preparation method of capsules in the pharmacopeia formulation, it is prepared in the following component content per capsule.

Example 1 Dry Powder 300 mg

Corn starch ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ ・ 100 mg

Lactose ····················· 100 mg

Magnesium stearate 2 mg

Formulation Example 4 Preparation of Injection

According to the preparation method of injection in the pharmacopeia formulation, it is prepared in the following component content per ampoules (2 ml).

Example 1 Dry Powder 300 mg

Sterile distilled water for injection ㆍ ·············

pH regulator ㆍ ··················

Formulation Example 5 Preparation of Liquid

According to the preparation method of the liquid formulation in the Pharmacopoeia General Formulation, it is prepared in the following component content per 100 ml of the liquid formulation.

Example 1 Dry Powder... 1 g

Heterosaccharides ················· 10 g

Mannitol ···················· 5 g

Purified water ㆍ ···················

In addition, the health beverage is prepared as follows.

5-10% of sugar, 0.05-0.3% citric acid, 0.005-0.02% caramel, 0.1-1% of vitamin C are mixed, and 79-94% of purified water is mixed to make syrup, and the syrup is 85-98 ° C. Sterilized at 20 ~ 180 seconds in a mixture of cooling water and a ratio of 1: 4, and then prepared a carbonated beverage containing a marginal excursion net source dry water extract by injecting carbon dioxide gas 0.5 ~ 0.82%.

Instant sterilization by homogeneously mixing subsidiary materials such as liquid fructose (0.5%), oligosaccharide (2%), sugar (2%), salt (0.5%), water (75%) and dry excipients After that, it was packaged in small packaging containers such as glass bottles and plastic bottles to prepare a healthy beverage.

Although the composition ratio is a composition suitable for a preferred beverage in a preferred embodiment, the compounding ratio may be arbitrarily modified according to regional and ethnic preferences such as demand hierarchy, demand country, and use purpose.

The composition containing the excipient excipient net extract, the prevention of diabetes mellitus and improve the concentration of total cholesterol, HDL, triglycerides due to diabetes has the activity of preventing and treating diabetes and its complications, thus preventing diabetes And as therapeutic agents.

1 is a result showing the effect on the weight change of the excursion excursion net origin, (○) is a non-treated group, (●) is a sample treatment group. Compared with the untreated group, P values were indicated by * below 0.05, below 0.01, and below 0.001 ***.

Figure 2 is a result showing the effect on the plasma glucose concentration of the excursion excursion source, and the meaning of (○), (●) and significance display is the same as the meaning in FIG.

Figure 3 is a result of measuring the effect on the glycated hemoglobin of the marginal excursion swangi source (○), (●) and the meaning of the significance display is the same as the meaning in FIG.

Figure 4 is a result of measuring the effect on the plasma insulin change of the excursions of the marginal excursions (○), (●) and the meaning of the significance display is the same as the meaning in FIG.

5 is a result of measuring the effect on the C-peptide of the excursion excursion source (○), (●) and the significance of the meaning is the same as the meaning in FIG.

Figure 6 is a result of measuring the effect on the total cholesterol concentration of the excursion excursion net source (○), (●) and the significance of the meaning is the same as the meaning in FIG.

Claims (8)

A pharmaceutical composition for the treatment of diabetes mellitus and its complications, comprising a combination herbal extract consisting of rhubarb, tea, earth and sand, fruit, wind, and poison as an active ingredient and mixed with a conventional pharmaceutically acceptable carrier. The method of claim 1, Complications caused by diabetes are diseases caused by elevated blood levels of triglycerides, cholesterol, and low density lipids. The method of claim 1, Composition comprising 0.5 to 50% by weight of the composite herbal extract based on the total weight of the composition. The method of claim 1, A composition comprising the weight ratios of two herbal medicines of rhubarb, tea and earthenware, and three herbal medicines of fruit room, windproof, and poisonous activity of about 1 to 10: 1 to 5: 1. The method of claim 3, wherein The composition of two herbal medicines of rhubarb, tea and earthenware, and three herbal medicines of fruit room, windproof, and poisonous activity, is about 3-7: 2-4: 1 The method of claim 4, wherein The composition of the two herbal medicines of rhubarb, tea and earthenware and three herbal medicines of fruit room, windproof, and poisonous activity is about 5: 2.5: 1. The method of claim 1, A pharmaceutical composition for treating diabetes, wherein the pharmaceutical form is pharmaceutically selected from powders, granules, tablets, capsules, liquids, and injections. delete