CN101732370B - Method for extracting an alpha-glucosidase active inhibitor from Limonium gmelinii(Willd.)Kuntze - Google Patents
Method for extracting an alpha-glucosidase active inhibitor from Limonium gmelinii(Willd.)Kuntze Download PDFInfo
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- CN101732370B CN101732370B CN200910249049XA CN200910249049A CN101732370B CN 101732370 B CN101732370 B CN 101732370B CN 200910249049X A CN200910249049X A CN 200910249049XA CN 200910249049 A CN200910249049 A CN 200910249049A CN 101732370 B CN101732370 B CN 101732370B
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Abstract
The invention discloses a method for extracting an alpha-glucosidase active inhibitor from Limonium gmelinii(Willd.)Kuntze that the Limonium gmelinii (Willd.)Kuntze is used as raw material; the product has the advantages of no toxic or side effect, high safety, low cost and industrial production. The method comprises the following steps of: cutting and crushing the Limonium gmelinii(Willd.)Kuntze, and putting into an extractor; adding an alcohol-water solution or an acetone-water solution, wherein the ratio of the volume of the alcohol-water solution or the acetone-water solution to mass ratio of the Limonium gmelinii(Willd.)Kuntze is 8-30 ml/g, and extracting by heating and refluxing, wherein the extracting temperature reaches to boiling temperature, the extracting is conducted once to three times, and each extracting lasts 1.0-3.0 h; collecting, and combining the cooled extracting solution, depressurizing to concentrate, freezing and drying to obtain the Limonium gmelinii(Willd.)Kuntze extract.
Description
Technical field:
The present invention relates to a kind of extracting method of alpha-glucosidase activity inhibitor, especially a kind of take the Limonium gmelinii (Wildl.) Kuntze herb as raw material, but the method for alpha-glucosidase activity inhibitor of extracting from Limonium gmelinii (Wildl.) Kuntze that product has no side effect, safe, the cheap industrialization of cost is produced.
Background technology:
Diabetes (Diabetes mellitus, DM) be a kind of metabolic disease group of multi-pathogenesis, mainly produce extremely relevant with effect with insulin, can cause sugar, fat, protein metabolism disorder, increase with glucose in urine as feature take blood glucose, and then cause the syndrome of a plurality of systems, a plurality of organ injuries, be the main cause that the mankind suffer from cardiovascular and cerebrovascular disease, blinding, amputation and renal failure.Having developed at present various new blood sugar lowering chemicals is applied to clinical, as improve glucose tolerance and insulin replies level insulin secretion stimulators (dipeptidyl peptidase enzyme DPP-IV inhibitor), improve glucose metabolism agent (biguanides), can effectively improve the metabolism of body polyalcohols, prevent and delay aldose reductase inhibitor (Pyrrolizidine lopps material) and the euglycemic agent that disease occurs.wherein euglycemic agent has become the research and development focus of present orally-taken blood sugar reducing medicine, can increase tissue to the sensitivity of insulin, alpha-glucosidase inhibitor particularly, as acarbose, its mechanism of action is Inhibiting α-glucosidase competitively, final step inhibition disaccharidase at carbohydrate digestion is degraded to monosaccharide, delay carbohydrate in the digestion of intestinal, delay is from disaccharidase, the glucose absorption of oligosaccharide and polysaccharide, effectively postpone and alleviate time and the process of after-dinner blood sugar of diabetes patients rising, it is the medicine of the control blood glucose commonly used of diabetes patient.
Although existing anti-diabetic kind is more, determined curative effect is can effectively control blood glucose, but be chemicals, exist action pathway single, even have serious untoward reaction, as causing hypoglycemia, lactic acidosis etc., long-term taking may with the drug effect reduction.
Limonium gmelinii (Wildl.) Kuntze Limonium gmelinii (Wildl.) Kuntz is the Bai Danhua Plumbaginaceae of section, and Herba Limonii Gmelinii Limonium Mill. belongs to herbaceos perennial.Limonnium contains the number of chemical composition, as aminoacid, vitamin, flavonoid, tannin, polysaccharide, alkaloid, organic acid etc., have enrich blood, hemostasis, anti-inflammation, the multiple efficacies such as scattered silt, regulating menstruation, strengthening the spleen, stomach invigorating, be mainly used in clinically at present treating cervical cancer and dysfunctional uterine hemorrhage disease, up to now also not about extract the alpha-glucosidase activity inhibitor relevant report from Limonium gmelinii (Wildl.) Kuntze.
Summary of the invention:
The present invention is in order to solve the existing above-mentioned technical problem of prior art, provide a kind of take the Limonium gmelinii (Wildl.) Kuntze herb as raw material, but the method for alpha-glucosidase activity inhibitor of extracting from Limonium gmelinii (Wildl.) Kuntze that product has no side effect, safe, the cheap industrialization of cost is produced.
Technical solution of the present invention is: a kind of method of extracting alpha-glucosidase activity inhibitor from Limonium gmelinii (Wildl.) Kuntze is characterized in that carrying out as follows:
A. get the Limonium gmelinii (Wildl.) Kuntze herb, put into extractor after shredding grinding, adding volume and Limonium gmelinii (Wildl.) Kuntze herb mass ratio is alcohol-water or the acetone-water solution soaking of 8~30ml/g, heating and refluxing extraction, extract temperature for reaching to boiling temperature, extraction time is 1~3 time, and each extraction time is 1.0~3.0h;
B. collect the cooled extracting solution of merging, concentrating under reduced pressure, lyophilization gets the Limonium gmelinii (Wildl.) Kuntze extract.
The determining alcohol of the alcohol-water solution of described a step is 70%~100%, and alcohol wherein is methanol, ethanol or propanol, the acetone concentration 50%~100% in described acetone-water solution.
The present invention is take wide material sources, cheap Limonium gmelinii (Wildl.) Kuntze as raw material, utilize solvent extraction method that the Limonium gmelinii (Wildl.) Kuntze herb is extracted, resulting extract suppresses experimental results show that through alpha-glucosidase activity, have the effect of significant Inhibiting α-glucosidase activity and have no side effect, safe, cost is cheap, can be widely used in preparing in the medicine and health product that reduces diabetes.The present invention is simple to operate, and solvent for use is general chemistry reagent, easily repeats, and is suitable for large-scale production.
Description of drawings:
Fig. 1 is the embodiment of the present invention 3 extract concentrations and the active corresponding curve chart of the inhibition of alpha-glucosidase.
The specific embodiment:
Embodiment 1:
A. accurately take Limonium gmelinii (Wildl.) Kuntze herb 10g, shred in the acetone-water solution that immerses 100ml 100% after grinding and spend the night, adopt the apparatus,Soxhlet's heating and refluxing extraction, extract temperature for reaching to boiling temperature, extraction time is 2 times, and each extraction time is 2h;
B. collect the cooled extracting solution of merging, rotate evaporation and concentration under 50 ℃, lyophilization gets the Limonium gmelinii (Wildl.) Kuntze extract.
Get the embodiment of the present invention 1 extract, the adding distil water standardize solution, compound concentration is the Limonium gmelinii (Wildl.) Kuntze extract solution of 3.125mg/mL, carry out alpha-glucosidase according to art methods and suppress activity experiment, recording suppression ratio is 76.04%, apparently higher than positive control--and acarbose (suppression ratio is 39.5% under equal concentrations).
Embodiment 2:
A. accurately take Limonium gmelinii (Wildl.) Kuntze herb 10g, shred in the acetone-water solution that immerses 300ml 50% after grinding and spend the night, adopt the apparatus,Soxhlet's heating and refluxing extraction, extract temperature for reaching to boiling temperature, extraction time is 3 times, and each extraction time is 1h;
B. collect the cooled extracting solution of merging, rotary evaporation is concentrated, and lyophilization gets the Limonium gmelinii (Wildl.) Kuntze extract.
Get the embodiment of the present invention 2 extracts, the adding distil water standardize solution, compound concentration is the Limonium gmelinii (Wildl.) Kuntze herb extract solution of 1.563mg/mL, carry out alpha-glucosidase and suppress activity experiment, recording suppression ratio is 56.3%, with concentration be the positive control of 6.25mg/mL--the suppression ratio (51.3%) of acarbose is suitable.
Embodiment 3:
A. accurately take Limonium gmelinii (Wildl.) Kuntze herb 10g, shred in ethanol (methanol or the propanol) solution that immerses 100ml 70% after grinding and spend the night, adopt the apparatus,Soxhlet's heating and refluxing extraction, extract temperature for reaching to boiling temperature, extraction time is 1 time, and each extraction time is 3h;
B. collect cooled extracting solution, rotate evaporation and concentration under 50 ℃, lyophilization gets the Limonium gmelinii (Wildl.) Kuntze extract.
Get the embodiment of the present invention 3 extracts, the adding distil water standardize solution, dilute successively that compound concentration is respectively 2,1,0.5, the Limonium gmelinii (Wildl.) Kuntze herb extract solution of 0.25mg/mL, carry out alpha-glucosidase according to the method for prior art and suppress activity experiment, record four extracts under concentration alpha-glucosidase is all had the activity of inhibition, its activity exists dose-dependence (seeing Fig. 1), IC
50Value is the 1.77mg/mL (IC of positive control drug acarbose
50Value is 6.25mg/mL).
The extract that the embodiment of the present invention 1~3 prepares can be used as the various preparations for the treatment of and prevent diabetes and complication medicine thereof, the health food that suppresses diabetes and complication thereof and conduct and falls hypoglycemic food additive etc.
The medicine for the treatment of and prevent diabetes and complication thereof or health food can mix with adjuvant forming various forms of pills, powder, tablet, unguentum, powder, injection, water preparation, granule, capsule, injection etc.; The health food form can be liquid such as the picture refreshment drink, soda pop, flavoring agent; As additive, such as joining the wheaten food class, fruit jelly, ice cream, curing food, Petaso, point is in the heart.
Claims (1)
1. method of extracting alpha-glucosidase activity inhibitor from Limonium gmelinii (Wildl.) Kuntze is characterized in that carrying out as follows:
A. get the Limonium gmelinii (Wildl.) Kuntze herb, put into extractor after shredding grinding, adding volume and Limonium gmelinii (Wildl.) Kuntze herb mass ratio is alcohol-water or the acetone-water solution soaking of 8~30ml/g, heating and refluxing extraction, extract temperature for reaching to boiling temperature, extraction time is 1~3 time, and each extraction time is 1.0~3.0h; The determining alcohol of described alcohol-water solution is 70%~100%, and alcohol wherein is methanol, ethanol or propanol, the acetone concentration 50%~100% in described acetone-water solution;
B. collect the cooled extracting solution of merging, concentrating under reduced pressure, lyophilization gets the Limonium gmelinii (Wildl.) Kuntze extract.
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