CN106038624A - Limonium franchetii antineoplastic active constituent extract and preparation method and application thereof - Google Patents
Limonium franchetii antineoplastic active constituent extract and preparation method and application thereof Download PDFInfo
- Publication number
- CN106038624A CN106038624A CN201610376979.1A CN201610376979A CN106038624A CN 106038624 A CN106038624 A CN 106038624A CN 201610376979 A CN201610376979 A CN 201610376979A CN 106038624 A CN106038624 A CN 106038624A
- Authority
- CN
- China
- Prior art keywords
- limonium
- franchetii
- extract
- tumor
- effective component
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses limonium franchetii antineoplastic active constituent extract and a preparation method and application thereof. According to the limonium franchetii antineoplastic active constituent extract, after alcohol extraction is carried out on limonium franchetii, four kinds of active extract including cyclohexane, ethyl acetate, n-butyl alcohol and water are obtained. The active extract of the limonium franchetii can restrain proliferation of various cancer cell strains, the restraining effect is remarkable especially for leukemia cells HL-60 and K562, breast cancer cells MCF-7, lung cancer cells A549, cervical cancer cells Hela, liver cancer cells HepG-2 and other tumor cell strains, the antineoplastic activity of the ethyl acetate extract is most obvious, the anticancer spectrum is wide, the limonium franchetii active extract has the effect of inducing differentiation of the leukemia cells HL-60, and therefore the limonium franchetii antineoplastic active constituent extract can be used for preparation and development of antineoplastic drugs and has important significance especially for treatment researches of leukemia.
Description
Technical field
The present invention relates to natural drug extract and application, the Limonium franchetii specifically referring to have anti-tumor activity carries
Take thing and its production and use.
Background technology
Cancer Mortality constantly raises, and has become as the mankind at present because of the main cause of disease death.Wherein, lung
Cancer, hepatocarcinoma and gastric cancer are the main entity tumor causes of death, and three accounts for the 60% of total mortality of malignant tumors, and leukemia
It it is the main malignant disease of harm blood system.It is radiotherapy, chemotherapy currently for the treatment means that malignant tumor is main, but putting
Treat side effect clinically and be easily generated greatly drug resistance so that clinical efficacy lowers.Therefore, new low toxicity, efficient, safe medicine are developed
Thing become treatment tumor in the urgent need to.The traditional medicine of China is with a long history, and a lot of Chinese medicines itself have anti-tumor activity, and
And regulation immunological function repression tumor can be played, there is the effect of wholistic therapy.High-efficiency low-toxicity is found from natural drug
The lead compound of anti-tumor activity, the focus of the most domestic and international cancer therapy drug research, and effective activated monomer and in
The anticancer compound preparation of medical herbs effective site all has important clinical value.
Limonium franchetiiLimonium franchetii(Debx.) Kuntze belongs to Plumbaginaceae
(Plumbaginaceae, also known as lumbaginaceae) Statice (LimoniumMiller), for Chinese unique wheat;Because of its corolla
Lavender (top is colourless sometimes), limb is faded to white by lilac red, is also called pale reddish brown Herba Limonii Gmelinii.This kind is rare
Kind, only it is distributed in Liaoning, the Shandong Peninsula to northeast of Jiangsu Province, is listed in country's second batch rare extinction plants.Enrich blood in the whole world
Grass platymiscium resource there are about 300 kinds, and China has 17 kinds, is distributed north and south each province.This platymiscium is containing flavone, alkaloid, polysaccharide, organic
The compositions such as acid, tannin, volatile oil, have enriching blood, stop blooding, the effect such as dissipating blood stasis, anti-inflammation and antitumor, and up-to-date research table
Bright Limonnium also have protect the liver, the pharmacologically active such as antiviral, Inhibiting α-glucosidase.Limonnium chemistry
The research of composition is focus in recent years, mainly has the research of limonium sinenseKuntze chemical composition, Herba Limonii Gmelinii with yellow flower volatile
Composition Study, Herba Limoii Bicoloris compounds content measure and the research etc. of compound extraction process.At present, there is no relevant Yantai
The document report of Herba Limonii Gmelinii chemical composition and pharmacologically active thereof, also has no and the Limonium franchetii extract preparation side in the present invention
The report that method, purposes are relevant.
Summary of the invention
Inventing the above-mentioned weak point that aims to overcome that of technical problem to be solved, research design Limonium franchetii carries
Take thing and extraction separation method thereof and the application in preparing antitumor drug.
It is an object of the invention to provide a kind of effective portion extracting from Limonium franchetii and there is antitumor action
Position.
The two of the purpose of the present invention are to provide a kind of simple preparation method extraction and obtain having anti-swelling in Limonium franchetii
The extract of tumor effect.
The three of the purpose of the present invention are to provide a kind of pharmaceutical composition with described position as effective ingredient.
The four of the purpose of the present invention are to provide the application in preparing antitumor and related drugs of the described effective site.
Technical scheme is as follows:
Limonium franchetii anti-cancer effective component extract, it is Limonium franchetii herb crushed after being dried, adds 95% ethanol leaching
Carrying three times, united extraction liquid, concentrating under reduced pressure, after filtrate boils off ethanol, add distilled water and be stirred into suspension, water layer is according to pole
Property ascending order, successively with hexamethylene, ethyl acetate, n-butanol extraction.By hexamethylene, ethyl acetate, n-butyl alcohol and
After water extract concentrating under reduced pressure recycling design respectively, obtain four position Limonium franchetii anti-cancer effective component extracts.
A kind of method preparing above-mentioned Limonium franchetii anti-cancer effective component extract, it comprises the following steps:
(1) Limonium franchetii is raw material, medical material Limonium franchetii crushed after being dried, adds 95% ethanol uniform stirring, stands 24h,
In triplicate, filter, merge and obtain extracting solution.
(2), after the extracting solution of step (1) concentrates and removes ethanol, obtain total extractum, add 2-5 times of volume of water and stir into suspension
Liquid, extracts Aqueous extracts with hexamethylene volume ratio 1:1, extracts three times, merges hexamethylene extract.Reclaim hexamethylene,
40 DEG C of vacuum driers are dried, and obtain hexamethylene position.
(3) water layer after being extracted by hexamethylene extracts with ethyl acetate volume ratio 1:1, extracts three times, merges extraction
Liquid.Reclaiming ethyl acetate, 30 DEG C of vacuum driers are dried, and obtain ethyl acetate extract.
(4) will extract with n-butyl alcohol volume ratio 1:1 through hexamethylene and ethyl acetate stripped water layer solution,
Extract three times, combining extraction liquid.Reclaiming n-butyl alcohol, 60 DEG C of vacuum driers are dried, and obtain n-butanol portion.
(5) water extract is used cryodesiccated method concentrate drying, obtain water position.Above four polar fractions are i.e.
For Limonium franchetii active component extract.
Invention further provides Limonium franchetii anti-tumor effective component extract in preparing antitumor drug
Application.With Human leukemia cell line HL-60 and K562, HepG2 cell lines, human A459 lung cancer cell line, people
Breast cancer cell MCF-7, human cervical carcinoma cell strain Hela are detection tumor strain, to suppress tumor cell proliferation ability as activity
Index carries out anti-tumor activity experiment.Test result indicate that, in vitro in cellulotoxic experiment, four positions can suppress tumor thin
The propagation of born of the same parents, has cytotoxicity, the anti-tumor activity that ethyl acetate extract performs better than.Further, hexamethylene position and water
Position shows as the activity of induction differentiation good to Human leukemia cell line HL-60, can be used for preparing antineoplaston medicine.
Present invention also offers the pharmaceutical composition of the effective site prepared in this way.Described pharmaceutical composition be with
The effective site of the present invention is effective ingredient, is formed with pharmaceutically acceptable carrier, and to be suitable for medicinal dosage form
Existing, these dosage forms can be tablet, sugar coated tablet, film coated tablet, enteric coated tablet, slow releasing tablet, capsule, hard
Capsule, soft capsule, slow releasing capsule, oral liquid, mixture, suck agent, granule, electuary, pill, powder, unguentum, pellet
Agent, suspensoid, solution, injection, injectable powder, lyophilized injectable powder, suppository, ointment, plaster, cream, spray, drip
Agent, drop pill and patch etc..The medicine of above-mentioned various dosage form all can be prepared according to the conventional method of pharmaceutical field.
Beneficial effect
(1) advantages of the present invention and effect are to use the conventional method such as solvent extraction, extraction, find Limonium franchetii ring first
Hexane position, ethyl acetate extract, n-butanol portion and water position have anti-tumor activity, and this active component the experiment proved that tool
There is the activity that extracorporeal suppression tumor cell grows.
(2) present invention firstly discovers that hexamethylene position and the water position of Limonium franchetii have induction human leukemia cell
The activity of HL-60 cell differentiation, suitably develops into Chinese medicine for treating leukemia new drug.
(3) present invention has expanded the raw material channel of antitumor drug, expands the purposes of Limonium franchetii, makes Yantai enrich blood
Grass develops into the new raw material of antitumor drug, is remarkably improved the medical value of Limonium franchetii.
Accompanying drawing explanation
Fig. 1 is the schematic diagram of Limonium franchetii extract anti-tumor activity experiment IC50 value (g/mL).
Fig. 2 is that Wright's staining observes the Limonium franchetii extract schematic diagram on the differentiation impact of HL-60 cell.
Detailed description of the invention
Set forth below being implemented with helps those skilled in the art and is more fully understood that the present invention, but limits this never in any form
Invention.
Embodiment 1: the preparation of Limonium franchetii anti-tumor effective component extract
By Limonium franchetii herb drying and crushing, take mixture 1500g, add 95% soak with ethanol 2-3 days of 8-10 times amount, extraction
Taking Alcohol soluble composition, in triplicate, merge 3 extracting solution, 30 DEG C ~ 40 DEG C decompression recycling ethanols, to without alcohol taste, obtain total extractum,
Add distilled water and be stirred into suspension, load in separatory funnel, add isopyknic hexamethylene and extract three times, merge hexamethylene extraction
Taking liquid, recovered under reduced pressure, 40 DEG C of vacuum driers are dried to constant weight, obtain hexamethylene position.The decompression of remaining water liquid removes residual
Hexamethylene after, with isopyknic ethyl acetate extract 3 times, combined ethyl acetate extract, recovered under reduced pressure, 30 DEG C of vacuum drying
Machine is dried to constant weight, obtains ethyl acetate extract.After remaining water layer solution decompression removes the ethyl acetate of residual, use equal-volume
N-butanol extraction 3 times, merge butanol extraction liquid, recovered under reduced pressure, 60 DEG C of vacuum driers are dried to constant weight, obtain n-butyl alcohol
Position.Finally water extract is used cryodesiccated method concentrate drying, obtains water position.
Embodiment 2: the research of Limonium franchetii extract anti-tumor activity
The Limonium franchetii anti-tumor effective component extract obtained with embodiment 1, as test medicine, takes and is in exponential phase
Kinds of tumor cells (tumor cell line of employing has the most several: Human leukemia cell line HL-60 and K562, people's hepatocarcinoma are thin
Born of the same parents strain HepG2, human A459 lung cancer cell line, human breast cancer cell line Bcap-37, human cervical carcinoma cell strain Hela), with containing 10%
The RPMI-1640 complete culture solution regulation cell concentration of calf serum, 8000, every hole cell 180 μ L is inoculated in 96
In porocyte culture plate, put 37 DEG C, 5%CO2 In incubator overnight, dosing next day.The sample solution of the different extracted parts is joined
Making the mother solution that initial concentration is 1mg/mL, two-fold dilution, every hole adds the medicine 20 μ L diluted, with PBS as feminine gender
Comparison.After sample-adding cultivates 72h, every hole adds the MTT 20 μ L of 5mg/mL, continues to cultivate 4 h, and then quickly turnover panel method is abandoned
Removing the DMSO that liquid in hole, every hole add 100 μ L, vibrate 10min, detects each hole in microplate reader at 570nm wavelength
OD value, and use the IC50 value of the GraphPad each extract part of Prism computed in software.The results are shown in Table 1.Can by result
Knowing, four positions of Limonium franchetii extract have good tumor inhibitory effect, with ethyl acetate extract antitumous effect
Substantially.
Embodiment 3: the impact that acute myeloid leukemia HL-60 cell induction is broken up by Limonium franchetii extract
The Limonium franchetii anti-tumor effective component extract obtained with embodiment 1 is as test medicine, with final concentration 25 μ g/mL
Different parts extract-treated be in the HL-60 cell of exponential phase, with 5 × 104Individual/mL cell concentration is laid on 24
Orifice plate, puts 37 DEG C, 5%CO2Cultivating 5d in incubator, collect each group of cell, smear, Wright's staining, basis of microscopic observation is thin
Born of the same parents' form is also taken a picture, the feature such as observation of cell size, karyoplasmic ratio, nuclear shapes, endochylema dyeing.Result shows, enriches blood in Yantai
After the extract-treated leukemia HL-60 cell 5d at four positions of grass, observation of cell morphology generation significant change under light microscopic
(see figure 1).Showing as nuclear chromatin condensation, caryoplasm ratio reduces, and core is partial to side, has depression and leaflet, and cell is to ripe rank
Section differentiation, especially obvious with hexamethylene position and ethyl acetate extract, illustrate that Limonium franchetii extract is thin to HL-60
Born of the same parents all have the ability of certain induction differentiation.Therefore, Limonium franchetii extract may be used for preparing anti-tumor drug, especially
Can be as differentiation-inducing agents for leukemic treatment.
Claims (9)
1. Limonium franchetii anti-cancer effective component extract, is characterized in that: Limonium franchetii herb crushed after being dried, adds
95% ethanol extraction, filtrate obtains extractum, water dissolution after boiling off ethanol, use hexamethylene, ethyl acetate, n-butanol extraction successively, dense
Contracting obtains position, Limonium franchetii hexamethylene position, ethyl acetate extract, n-butanol portion and position after drying, mends for Yantai
Blood grass anti-cancer effective component extract.
A kind of Chinese medicine extract with anti-tumor activity the most according to claim 1, it is characterised in that be extracted described in:
Limonium franchetii be through soak with ethanol and be dried herb, including root, stem, Ye Hehua.
Application the most according to claim 1, it is characterised in that described Limonium franchetii active component extract is Yantai
Cyclohexane thing, ethyl acetate extract, n-butanol extract or the water extract of Herba Limonii Gmelinii.
4. the method preparing Limonium franchetii anti-tumor effective component extract described in claim 3, is characterized in that it
Comprise the steps:
Step 1: Limonium franchetii herb is pulverized, with 95% alcohol steep three times, united extraction liquid, concentrating under reduced pressure removes ethanol,
Obtain total extractum；
Step 2: total extractum step 1 obtained, adds distilled water and is stirred into suspension, load in separatory funnel, and water layer is pressed
According to the order that polarity is ascending, extract with hexamethylene, ethyl acetate, butanol solution successively.By hexamethylene, acetic acid second
After ester, n-butyl alcohol and water extract rotary evaporation concentrating under reduced pressure respectively, obtain Limonium franchetii anti-tumor effective component extract.
5. the application in preparing antitumor drug of the Limonium franchetii anti-tumor effective component extract described in claim 1.
Limonium franchetii anti-tumor effective component extract the most according to claim 5 answering in preparing antitumor drug
With, it is characterized in that: described antineoplastic tumor cell is leukemia HL-60 and K562 cell, MCF-7 Breast Cancer Cell, cervix uteri
Cancer Hela cell and HepG-2 cell line, Limonium franchetii extract can suppress Proliferation of Tumor Cells In Vitro.
Limonium franchetii anti-tumor effective component extract the most according to claim 5 answering in preparing antitumor drug
With, it is characterised in that described Limonium franchetii extract suppresses leukemia HL-60 and K562 cell proliferation in vitro, also may be used
With inducing leukemia cell HL-60 and K562 directed differentiation.
Limonium franchetii anti-tumor effective component extract the most according to claim 5 answering in preparing antitumor drug
With, it is characterized in that: described antitumor drug is that Limonium franchetii anti-tumor effective component extract is with pharmaceutically acceptable
Carrier or adjuvant composition.
Limonium franchetii anti-tumor effective component extract the most according to claim 5 answering in preparing antitumor drug
With, it is characterised in that: described pharmaceutical composition is with the effective site of the present invention as effective ingredient, with pharmaceutically acceptable load
Body is formed, and exists to be suitable for medicinal dosage form, and these dosage forms can be tablet, sugar coated tablet, Film coated tablets
Agent, enteric coated tablet, slow releasing tablet, capsule, hard capsule, soft capsule, slow releasing capsule, oral liquid, mixture, suck
Agent, granule, electuary, pill, powder, unguentum, sublimed preparation, suspensoid, solution, injection, injectable powder, lyophilized injectable powder, bolt
Agent, ointment, plaster, cream, spray, drop, drop pill and patch etc..
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610376979.1A CN106038624B (en) | 2016-06-01 | 2016-06-01 | Yantai herba limonii gmelinii anti-cancer effective component extract and its preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610376979.1A CN106038624B (en) | 2016-06-01 | 2016-06-01 | Yantai herba limonii gmelinii anti-cancer effective component extract and its preparation method and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106038624A true CN106038624A (en) | 2016-10-26 |
| CN106038624B CN106038624B (en) | 2019-11-29 |
Family
ID=57172346
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610376979.1A Active CN106038624B (en) | 2016-06-01 | 2016-06-01 | Yantai herba limonii gmelinii anti-cancer effective component extract and its preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106038624B (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102920753A (en) * | 2012-11-05 | 2013-02-13 | 江苏省中国科学院植物研究所 | Dichromatism gmelin sealavender herb extractive and application thereof |
| CN105362314A (en) * | 2015-12-09 | 2016-03-02 | 江苏省中国科学院植物研究所 | Novel application of Limonium bicolor ethyl acetate extract |
-
2016
- 2016-06-01 CN CN201610376979.1A patent/CN106038624B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102920753A (en) * | 2012-11-05 | 2013-02-13 | 江苏省中国科学院植物研究所 | Dichromatism gmelin sealavender herb extractive and application thereof |
| CN105362314A (en) * | 2015-12-09 | 2016-03-02 | 江苏省中国科学院植物研究所 | Novel application of Limonium bicolor ethyl acetate extract |
Non-Patent Citations (3)
| Title |
|---|
| 丁鸽等: "补血草属植物野生资源多样性及药理学研究进展", 《时珍国医国药》 * |
| 杨志强等: "《春曲：中国农业科学院兰州畜牧与兽药研究所第二届中青年科技论文暨盛彤笙杯演讲比赛论文集》", 31 July 2009, 中国农业科学技术出版社 * |
| 许继宏: "《药用植物组织培养技术》", 31 May 2003, 中国农业科学技术出版社 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106038624B (en) | 2019-11-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103316096A (en) | General flavone extract of seeds of nigella damascena l., nigella sativa l. or nigella glandulifera freyn et sint., and preparation method and use thereof | |
| CN112472729B (en) | Application of caulis sinomenii in preparing medicine for treating human glioma | |
| CN105153084A (en) | Novel diterpene compound as well as preparation method and medicinal application thereof | |
| CN106176716B (en) | The new application of daphane diterpene compound pimelotide C | |
| CN101157717B (en) | Preparation method of Ardisia mamillata B and uses thereof | |
| CN108434399A (en) | A kind of Chinese medicine composition and preparation method of anti-curing oncoma | |
| CN100381434C (en) | Emblic leafflower fruit extract possessing anticancer, antibiotic actions and its manufacturing method of traditional Chinese medicine formulation | |
| CN101849950A (en) | Application of rotundic acid in preparing blood lipid regulating medicines | |
| CN107753531B (en) | Wenwang-herba violae extract and application thereof in preparing anti-cancer drugs | |
| CN103408528B (en) | Chroman compound, as well as preparation method and application thereof | |
| CN102988529A (en) | Preparation method and novel application of total phenolic acid in cherry stones | |
| CN103893412B (en) | A kind of antitumor beautyberry extract and its production and use | |
| CN101085804A (en) | Method for preparing lonicera macranthoides hypo-saponin B and application of the same in curing liver cancer, breast carcinoma and cervical cancer | |
| CN101721434B (en) | Active ingredients of fomes officinalis, preparation method thereof and use thereof | |
| CN106038624A (en) | Limonium franchetii antineoplastic active constituent extract and preparation method and application thereof | |
| CN103142934A (en) | Traditional Chinese medicinal composition for treating lung cancer and liver cancer | |
| CN102232988A (en) | Total saponins of psammosilene tunicoids (TSPT) with antitumor activity, and preparation thereof | |
| CN101735189A (en) | Preparation method, preparation and application of tricin | |
| CN101612181A (en) | Cerbera manghas L. and crude extract thereof the new purposes in the preparation antitumor drug | |
| CN104529968B (en) | Anti-tumor diterpenoid compound, and pharmaceutical composition, preparation method and application thereof | |
| CN103450142B (en) | Chroman compound as well as extracting method and application thereof | |
| CN103751269A (en) | Application of herba rabdosiae rubescentis extract to alpha-glucosidase inhibitor | |
| CN104147565B (en) | Extraction method for mioga ginger antineoplastic active component | |
| CN108853308A (en) | A kind of Chinese paris rhizome total saponin-semen momordicae pharmaceutical composition and preparation method | |
| CN102068537B (en) | The preparation of Pericarpium Citri Reticulatae Radix Glycyrrhizae prevents and treats the health food of nasopharyngeal carcinoma and the production method of medicine |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20221021 Address after: 262400 Nine-level Village South of Zhuliu Street, Changle County, Weifang City, Shandong Province Patentee after: SHANDONG GUANGYUAN PHARMACEUTICAL TECHNOLOGY Co.,Ltd. Address before: 264025 No. 186 Hongqi Middle Road, Zhifu District, Shandong, Yantai Patentee before: LUDONG University |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20221214 Address after: 271100 50m north of Huitong West Road, Kouzhen sub district office, Laiwu District, Jinan City, Shandong Province Patentee after: SHANDONG JINZHUJI PHARMACEUTICALS Co.,Ltd. Patentee after: SHANDONG GUANGYUAN PHARMACEUTICAL TECHNOLOGY Co.,Ltd. Address before: 262400 Nine-level Village South of Zhuliu Street, Changle County, Weifang City, Shandong Province Patentee before: SHANDONG GUANGYUAN PHARMACEUTICAL TECHNOLOGY Co.,Ltd. |