CN101612181A - Cerbera manghas L. and crude extract thereof the new purposes in the preparation antitumor drug - Google Patents
Cerbera manghas L. and crude extract thereof the new purposes in the preparation antitumor drug Download PDFInfo
- Publication number
- CN101612181A CN101612181A CN200910145538A CN200910145538A CN101612181A CN 101612181 A CN101612181 A CN 101612181A CN 200910145538 A CN200910145538 A CN 200910145538A CN 200910145538 A CN200910145538 A CN 200910145538A CN 101612181 A CN101612181 A CN 101612181A
- Authority
- CN
- China
- Prior art keywords
- extract
- cerbera
- manghas
- cerbera manghas
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses Cerbera manghas L. and extract thereof the new purposes in the preparation antitumor drug, belong to Chinese medicine, natural medicine field.Cerbera manghas L. passes through ethanol extraction, extract with petroleum ether, remaining water liquid is gone up macroporous adsorbent resin (D101) column chromatography after filtering, priority water and methanol-eluted fractions, collect meoh eluate, obtain the methanol position through concentrating, the experiment proved that, extract the effect that resulting methanol position has antitumor cell.
Description
Technical field
The present invention relates to a kind of medicinal plants new medical application, especially Cerbera manghas L. (Cerbera manghas Linnaeus) and extract thereof the new purposes in the preparation antitumor drug, belong to Chinese medicine, natural medicine field.
Background technology
Cerbera manghas L. (Cerbera manghas Linnaeus) claim extra large Fructus Mangifera Indicae again, another name mountain appearance, monkey joyful (Taiwan), gold eggplant (Qionghai), cattle gold eggplant, Cor Bovis seu Bubali litchi, gold are transferred (Wenchang), and (Apocynaceae) Cerbera manghas L. belongs to (Cerbera Linnaeus) plant for Apocynaceae.About 9 kinds of Cerbera manghas L. platymiscium is distributed in Asia and Australian torrid areas; China produces a kind, and Ji Cerbera manghas L. C.manghas is distributed in ground such as Guangdong, Guangxi, Taiwan.Its bark and milk are medicinal, and effect emetic, that drop is arranged.Cure mainly the acute case of heart failure, may be better than ouabain.Also be used as analgesia, convulsion, heart tonifying and hypotensor.
At present, Cong the Cerbera manghas L. plant, isolated 90 surplus kind of chemical compound, wherein effective ingredient is mainly cardiac glycoside compounds.Chang L C. (2000) etc. assigns to 5 chemical compounds Cong the Cerbera manghas L. root, wherein 2 new cardiac glycosidees have obvious anti-tumor activity.(Chang Leng Chee, Gills Joell J, Bhat Krishna P L, et al.Bioorgganic﹠amp; Medicinal ChemistryLetters, 2000,10 (21): 2431～2434), " a class cerberine derivant, Preparation Method And The Use " (patent C07J 71/00, A61K 31/704, A61P 35/00) relates to a class and extract, separate the new extra large Fructus Mangifera Indicae analog derivative Preparation method and use with remarkable anti-tumor activity of acquisition etc. from the Fructus Mangifera Indicae of mangrove plant sea.
Cancer is one group of common name that can influence more than the 100 kind of disease at any position of health.Other term that uses is malignant tumor and tumor.Cancer is the major causes of death in the whole world.Die ten thousand deaths in the whole world 5800 in 2005 and to die in the sum, cancer accounts for 7,600,000 (or 13%) of all death.Cause the main cancer kind of cancer general mortality rate to be: lung (130 die ten thousand deaths dies/year), stomach (about 100 die ten thousand deaths dies/year), liver (662 000 death/year), colon (655 000 death/year), mammary gland (502 000 death/year).Present treatment means is that clinical application carries out the transition to the multiple medicines combined chemotherapy from the single medicine treatment, but, most treatments only can prolong life and can not eradicate disease, many toxic and side effects such as liver failure and the diabetes etc. of following treatment to produce make patient's immune system suffer damage.From natural drug, seek the heat subject that antitumor drug is research at present.The extract of some Chinese herbal medicine such as enmein (" Yunnan plant research ", 2006,28:95), apigenin-8-C-glucoside (" tropical and subtropical zone Botany Gazette ", 2008,16:51), linoleic acid (" shanghai Medicine ", 1994,17:635) grade has the antineoplastic activity, and its chemical constitution comprises diterpene, flavonoid glycoside, organic acid etc.Bulbus Allii, mushroom, Ganoderma and Radix Ginseng etc. are used for treatment of cancer by many countries.
Cerbera manghas L. of the present invention and extract thereof the new purposes in the preparation antitumor drug is not seen bibliographical information and patent disclosure so far as yet.
Summary of the invention
The purpose of this invention is to provide the new medical application of Cerbera manghas L., Ji Cerbera manghas L. and extract thereof the purposes in the preparation antitumor drug.
Content Shi Cerbera manghas L. of the present invention and extract thereof the purposes in the preparation antitumor drug, described purposes is characterised in that: 1, the Cerbera manghas L. extract can obtain: Cerbera manghas L. dry root by following step, suitably pulverize the back and doubly measure 95% ethanol soaking at room temperature extraction 3 times with 8-11, also but percolation extracts, and the extracting solution decompression recycling ethanol is not distinguished the flavor of to there being alcohol.Ethanol extraction is scattered in becomes suspension in the water, obtain the petroleum ether extractum A through petroleum ether extraction, remaining water liquid is gone up macroporous adsorbent resin (D-101) column chromatography, successively water and methanol-eluted fractions after filtering, collect meoh eluate, obtain methanol extractum B through concentrating.Extractum A, B are drying to obtain Cerbera manghas L. extract A, Cerbera manghas L. extract B; 2, Cerbera manghas L. extract B and/or Cerbera manghas L. 95% ethanol extraction add suitable pharmaceutic adjuvant, and preparation process routinely promptly can be made into oral drug preparation and injectable drug preparations such as tablet, capsule, granule, drop pill, oral liquid.
The Cerbera manghas L. extract B is used the cardiac glycoside chromogenic reagent through thin layer chromatography, shows that the Cerbera manghas L. extract B contains cardiac glycoside compounds.
Cerbera manghas L. extract B of the present invention, through the external activity screening, the statistical disposition result shows better antitumor activity.For further checking Cerbera manghas L. and extract thereof are in the effect aspect the treatment tumor, Dui Cerbera manghas L. extract B has been carried out the preliminary clinical observation on the therapeutic effect of 100 routine patients, and the result shows that the Cerbera manghas L. extract B is effective.The external activity screening and the preliminary clinical observation on the therapeutic effect result of patient of You Cerbera manghas L. extract B, can rationally derive Cerbera manghas L. and extract thereof can be used for preparing antitumor drug, Ji Cerbera manghas L. and extract thereof the purposes aspect the preparation antitumor drug.
Beneficial effect of the present invention, provide Cerbera manghas L. among the people be generally used for preparing treatment emetic, drop and conventionally be used to ease pain, new medical application Ji Cerbera manghas L. beyond the medicines such as convulsion, heart tonifying and blood pressure lowering and extract thereof be in the purposes aspect the preparation antitumor drug, and adopt different solvent extractions to obtain Cerbera manghas L. extract B Cerbera manghas L. extract B and add suitable pharmaceutic adjuvant, preparation process routinely promptly can be made into oral and the injectable drug preparation.
Preliminary clinical observation on the therapeutic effect test of the external activity screening of following Shi Cerbera manghas L. extract B and patient and result thereof.
One, external activity screening
Test method:
Adopt mtt assay to test the external antitumor activity (the results are shown in subordinate list 1) of Cerbera manghas L. extract B to human oral cavity epidermal carcinoma cell KB, breast carcinoma BC and pulmonary carcinoma NCI-H187, human colon cancer (Col2), carcinoma of endometrium cell strains such as (Ishikawa).Activity test method is as follows: the testing sample of negative control group (water), DMSO solvent control group, positive controls (ametycin) and 8 variable concentrations (0.8,1.6,3.2,6.3,12.5,25,50,100) is established in experiment, each concentration establish 2 parallel.Collect the exponential phase cell, the blood counting chamber counting is inoculated in the flat Tissue Culture Plate in 96 holes by 4500 the cancerous cell amounts in every hole, places 5%CO
2, humidity more than 90%, cultivate in 37 ℃ of incubators.Take out behind the 24h and add a certain amount of testing sample, taking-up places microscopically to observe every porocyte form after continuing to cultivate 3d, record cellular morphology situation of change, follow MTT solution (being dissolved in balanced salt solution PBS) the 50 μ L that every hole adds 5mg/mL, behind 37 ℃ of reaction 4h, with the cell culture fluid sucking-off, every hole adds 100 μ L DMSO Formazane is fully dissolved, Tissue Culture Plate is placed on the MK3 microplate reader, survey the absorbance (A) in each hole with the 570nm wavelength, by the following formula long suppression ratio of seeking survival.
With the sample concentration is abscissa, is vertical coordinate with the suppression ratio, maps and obtains the concentration (IC that suppression ratio is 50% o'clock sample
50), the active result of sample is promptly with half-inhibition concentration (IC
50) expression.
Experimental result:
Subordinate list 1 sample is to the toxic action of tumor cell lines such as SGC-7901, K562
Annotate: toxicity value IC
50, promptly the medicine pair cell is killed 50% o'clock concentration value.IC
50It is big more to be worth more little then toxicity.
Two, preliminary clinical observation on the therapeutic effect
Object of study: tumor patient, wherein male 34 examples, women 66 examples, age reckling 34 years old, the maximum 65 years old.
Diagnostic criteria: 1, do not have other any ill symptomses; 2, do not take any other antitumor drug.
Research method: this test is an object of study with 100 tumor patients, is tried thing and takes for patent medicine of the present invention (No. 0 capsule that contains 0.15 Cerbera manghas L. extract B approximately), and three times on the one, each two, boiled water is taken after mixing it with water.Per 40 days is a course of treatment, and drug withdrawal is had a rest a week after continuous two courses of treatment, observes change of illness state inspection in detail.Carry out the 3rd course of therapy then, withdraw other medicine at period in a medicine.
Clinical cure: the positive sign of symptom and objective determination disappears, and does not have the recidivist continuously half a year.
Clinical significantly: symptom disappears substantially, and cancer dwindles over half, and other objective determination takes an evident turn for the better, and observes more than half a year no longer development person.
Clinical effective: symptom makes moderate progress, and focus is basicly stable, observes person more than month.
Clinical invalid: symptom and objective determination do not have improvement, or only have short-term to improve, and deterioration person rapidly.
Efficacy result:
Advantage of the present invention is:
1, the natural drug selected for use of the present invention is a raw material, low toxicity, and side effect is little.
2, the present invention need not decoct, and treatment is convenient, and effective percentage reaches 91%.
3, the present invention can the control cancer cell hypertrophy, prevents the cancerous cell diffusion, makes the withered disappearance that comes off of cancerous cell.
4, the invention provides low dosage (0.5～2mg/kg) can produce anti-tumor activity, thus long-term low dose ((5～10mg/kg) antitumor action is stronger than high dose as 0.6～1.25mg/kg).
The specific embodiment
Embodiment 1
Qu Cerbera manghas L. dry product 2.0kg divides 3 (5,3,3 days) lixiviates with 10 times of amount 95% ethanol, decompression recycling ethanol, and concentrate drying obtains extract powder, and it is an amount of that extract powder adds starch, is mixed, granulates, dry, granulate, incapsulates, and makes 1000 and get final product.
Embodiment 2
Qu Cerbera manghas L. dry product 10.0kg, with 9 times of amount 90% ethanol percolations, the concentrating under reduced pressure percolate to do not have alcohol distinguish the flavor of concentrated solution, concentrated solution is scattered in and becomes suspension in the water, obtains the petroleum ether extractum A through petroleum ether extraction, and remaining water liquid is gone up macroporous adsorbent resin (D-101) column chromatography after filtering, priority water and methanol-eluted fractions, collect meoh eluate, obtain methanol extractum B through concentrating, extractum B is drying to obtain the Cerbera manghas L. extract B.Qu Cerbera manghas L. extract B 100g adds appropriate amount of auxiliary materials such as starch, is mixed, granulates, drying, is pressed into 1000 and gets final product.
Embodiment 3
Qu Cerbera manghas L. dry product 5.0kg, with 8 times of amount 80% ethanol percolations, the concentrating under reduced pressure percolate to do not have alcohol distinguish the flavor of concentrated solution, concentrated solution is scattered in and becomes suspension in the water, obtains petroleum ether extractum through petroleum ether extraction, and remaining water liquid is gone up macroporous adsorbent resin (D-101) column chromatography after filtering, priority water and methanol-eluted fractions, collect meoh eluate, obtain methanol extractum through concentrating, the methanol extract dry is to De the Cerbera manghas L. extract B; Qu Cerbera manghas L. extract B 25g adds an amount of water for injection dissolving, and hydro-acupuncture preparation technology routinely transfers pH value, filtration, embedding, sterilization to make 1000ml and get final product; Perhaps add an amount of additives such as mannitol, dissolving stirs evenly, and filtration, lyophilizing, packing are made 500 bottles and got final product.
Claims (4)
1,, Cerbera manghas L. is in the new purposes of preparation in the antitumor drug, it is characterized in that described Cerbera manghas L. (Cerbera manghas Linnaeus) claims extra large Fructus Mangifera Indicae again, (Apocynaceae) Cerbera manghas L. belongs to (Cerbera Linnaeus) plant, medicinal Herb for Apocynaceae.
2,, the Cerbera manghas L. extract is in the new purposes of preparation in the antitumor drug, it is characterized in that the Cerbera manghas L. extract can be by following step acquisition:
The Cerbera manghas L. dry root suitably pulverizes the back and doubly measure 95% ethanol soaking at room temperature extraction 3 times with 8-11, but also percolation extracts, and the extracting solution decompression recycling ethanol is not distinguished the flavor of to there being alcohol.Ethanol extraction is scattered in becomes suspension in the water, obtain the petroleum ether extractum A through petroleum ether extraction, remaining water liquid is gone up macroporous adsorbent resin (D-101) column chromatography, successively water and methanol-eluted fractions after filtering, collect meoh eluate, obtain methanol extractum B through concentrating.Extractum A, B are drying to obtain Cerbera manghas L. extract A, Cerbera manghas L. extract B.
3,, it is characterized in that described Cerbera manghas L. extract B contains cardiac glycoside compounds according to the described Cerbera manghas L. extract of claim 2.
4, according to claim 1,2 described purposes, it is characterized in that: is Jiang Cerbera manghas L. extract B and/or Cerbera manghas L. 95% ethanol extraction, add suitable pharmaceutic adjuvant, preparation process routinely promptly can be made into oral drug preparation and injectable drug preparations such as freeze-dried powder, liquid drugs injection such as tablet, capsule, granule, drop pill, oral liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910145538A CN101612181A (en) | 2009-05-26 | 2009-05-26 | Cerbera manghas L. and crude extract thereof the new purposes in the preparation antitumor drug |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910145538A CN101612181A (en) | 2009-05-26 | 2009-05-26 | Cerbera manghas L. and crude extract thereof the new purposes in the preparation antitumor drug |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101612181A true CN101612181A (en) | 2009-12-30 |
Family
ID=41492189
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200910145538A Pending CN101612181A (en) | 2009-05-26 | 2009-05-26 | Cerbera manghas L. and crude extract thereof the new purposes in the preparation antitumor drug |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101612181A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103784357A (en) * | 2012-10-29 | 2014-05-14 | 伽蓝（集团）股份有限公司 | Mango active part and applications of the mango active part in preparation of whitening cosmetics |
| US8986750B2 (en) | 2010-09-17 | 2015-03-24 | Jiangzhong Pharmaceutical Co., Ltd. | Use of a traditional chinese medicine composition for manufacturing a health food or medicament for preventing and alleviating physical fatigue |
| CN107478649A (en) * | 2017-09-04 | 2017-12-15 | 中国热带农业科学院热带作物品种资源研究所 | Phenol compound is as the purposes of A type cardiac glycoside developer and the assay method of A type cardiac glycoside content |
-
2009
- 2009-05-26 CN CN200910145538A patent/CN101612181A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8986750B2 (en) | 2010-09-17 | 2015-03-24 | Jiangzhong Pharmaceutical Co., Ltd. | Use of a traditional chinese medicine composition for manufacturing a health food or medicament for preventing and alleviating physical fatigue |
| CN103784357A (en) * | 2012-10-29 | 2014-05-14 | 伽蓝（集团）股份有限公司 | Mango active part and applications of the mango active part in preparation of whitening cosmetics |
| CN103784357B (en) * | 2012-10-29 | 2016-05-18 | 伽蓝（集团）股份有限公司 | A kind of mango active site is in the application of preparing in skin-lightening cosmetic |
| CN107478649A (en) * | 2017-09-04 | 2017-12-15 | 中国热带农业科学院热带作物品种资源研究所 | Phenol compound is as the purposes of A type cardiac glycoside developer and the assay method of A type cardiac glycoside content |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103127190A (en) | Preparation method of domestic Brazilian ginseng extract, and application thereof in aspects of medicine and health care | |
| CN102370695B (en) | Qinglongyi active extract, its preparation method and its application | |
| CN101352468A (en) | Novel uses of Calotropis gigantea and extract thereof in preparing anti-tumor medicament | |
| CN101502579A (en) | Chinese medicinal composition for treating digestive tumor and preparation method thereof | |
| CN102617698B (en) | Method for preparing fine dioscin and application of fine dioscin | |
| CN104013636A (en) | Anti-tumor pharmaceutical use of pentacyclic triterpene saponin compounds of szechuan melandium root | |
| CN102068584B (en) | A kind of Dioscorea zingiberensis hydrophobicity steroidal saponin extract and its production and use | |
| CN103735653B (en) | A kind of Chinese medicine extract with anti-tumor activity and its production and use | |
| CN101612181A (en) | Cerbera manghas L. and crude extract thereof the new purposes in the preparation antitumor drug | |
| CN1861104B (en) | Inula flower extractive used to breat diabets mellitus and hyperlipidemia | |
| KR0160108B1 (en) | Anticancer agent of raw ingredient extracted from the tree named gleditschia officinalis | |
| CN101961366A (en) | Preparation method of malan straw extract, malan straw product, pharmaceutical composition and application thereof | |
| CN101229212A (en) | Lonicera fulvotnetosa total secondary saponin position, preparing method and antitumor uses thereof | |
| CN103893412B (en) | A kind of antitumor beautyberry extract and its production and use | |
| CN107753531B (en) | Wenwang-herba violae extract and application thereof in preparing anti-cancer drugs | |
| CN103142934B (en) | Traditional Chinese medicinal composition for treating lung cancer and liver cancer | |
| CN101077873B (en) | Novel NEO-clerodane type diterpene compound and application thereof | |
| CN103142935A (en) | Traditional Chinese medicinal composition for treating lung cancer and liver cancer | |
| CN101433575B (en) | Chinese sapium bark seed extract and preparation method thereof | |
| CN101948473A (en) | New NEO-clerodane diterpenoid compound and application thereof | |
| CN101007052A (en) | An antitumor medicine and its preparation method | |
| CN102988525A (en) | Preparation method for total lignans in hawthorn seeds, and novel application | |
| CN102293796A (en) | Active part of lysimachia trientaloides Hemsl., and extraction method and application of active part | |
| CN101732419A (en) | Application and preparation method of extract from fructus polygoni orientalis | |
| CN101690738A (en) | New application of harrisonia perforata and extractive thereof in preparing antitumor medicine |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20091230 |