KR101813606B1 - Cosmetic composition for skin whitening containing extracts of Indian natural substances - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin whitening, and more particularly to a cosmetic composition for whitening skin containing an Indian Bethelium resin extract and a Gadja extract. Further, the present invention relates to a cosmetic composition which inhibits melanin production and provides a skin whitening effect by containing an Indian Bethelium resin extract, a Gadja extract, a Winter cherry root extract, a Tengshin extract and an Indian gooseberry extract.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cosmetic composition containing natural products of India,

The present invention relates to a cosmetic composition for skin whitening, and more particularly, to a cosmetic composition for whitening containing an Indian Bethelium resin extract and a Gossamer extract. Further, the present invention relates to a cosmetic composition which inhibits melanin production and provides a whitening effect to the skin by containing an Indian Bethelium resin extract, Gadja extract, Winter cherry root extract, Tessin extract and Indian gooseberry extract.

Several factors are involved in determining the skin color of a person, including the activity of the melanocyte that makes the melanin pigment, the distribution of blood vessels, the thickness of the skin, and the presence or absence of pigment in and out of the body, such as carotenoids and bilirubin It is important.

Especially, the most important factor is melanin, a melanin pigment produced by various enzymes such as tyrosinase in melanocytes in the human body. The formation of melanin pigment affects genetic factors, hormonal secretion, physiological factors such as stress, and environmental factors such as ultraviolet irradiation.

The melanin pigment produced in melanocytes of body skin is a phenolic polymer substance having a complex form of black pigment and protein. It protects the subcutaneous skin organs by blocking ultraviolet rays irradiated from the sun, and at the same time, free radicals And protects proteins and genes in the skin.

Melanin, which is produced by stress stimuli inside and outside the skin, is a stable substance that does not disappear until the skin is excreted through skin keratinization even if the stress disappears. However, when melanin is produced more than necessary, it induces hypercholesterolemia such as spots, freckles, and dots, resulting in poor cosmetic results.

Today, oriental women prefer white, clean skin like white whites, and this is an important criterion of beauty, so there has been a growing demand for treatment and cosmetic problems for hyperpigmentation.

In response to such demands, substances having ascorbic acid, kojic acid, arbutin, hydroquinone, glutathione, derivatives thereof, or tyrosinase inhibiting activity have been conventionally used in cosmetics or pharmaceuticals, The use thereof is limited due to a whitening effect, a safety problem to the skin, a problem of formulation and stability in combination with cosmetics, and the like.

The inventors of the present invention have repeatedly and variously experimented and found that the extracts of Indian Bethelium resin and Gadja extract, and furthermore the extracts of Indian Bethelium resin and Gadja, among the natural products of India, further contain Winter cherry root extract, Tepsi extract and Indian gooseberry extract The present inventors confirmed that the cosmetic composition of the present invention can provide an excellent whitening effect while being safe to the skin, and completed the present invention.

Accordingly, an object of the present invention is to provide a whitening cosmetic composition containing an Indian Bethelium resin extract and a Gadja extract.

In order to attain the above object, the present invention provides a cosmetic composition for whitening comprising an Indian Bethelium resin extract and Gadja extract.

The present invention also provides a cosmetic composition for whitening, which further comprises a winter cherry root extract, a tsunami extract, and an Indian gooseberry extract in an Indian Bethelium resin extract and a Gadja extract.

The composition of the present invention exhibits a skin whitening effect by containing an Indian Bethelium resin extract, a Gadja extract, a Winter cherry root extract, a Ganoderma extract and an Indian gooseberry extract, and thus can be used in various fields in the field of cosmetics.

FIG. 1 is a graph comparing the whitening effect of each of the Indian Bethelium resin extract, Gadja extract, Winter cherry root extract, Tepsi extract, and Indian gooseberry extract according to Test Example 1.
FIG. 2 is a graph showing the whitening effect and the whitening effect of the Indian Betelrium resin extract and the Gadja extract mixed at various ratios according to Test Example 1 and the Indian Bethelium resin extract, Gadja extract, Winter cherry root extract, This is a graph comparing the whitening effect of the extract.

The present invention provides a cosmetic composition for whitening skin comprising an Indian Bethelium resin extract and a Gadja extract as an active ingredient.

The present invention also provides a cosmetic composition for skin whitening comprising as an active ingredient an Indian Bethelium resin extract, a Gadja extract, a Winter cherry root extract, a Ganoderma extract, and an Indian gooseberry extract.

Hereinafter, the present invention will be described in more detail.

Among the natural products of India contained as an active ingredient in the present invention, the Commiphora Mukul Resin has been used for about 3,000 years in the Indian traditional Ayurvedic medicine system originating in India, Northeast Africa and Afghanistan. It produces natural resin called google gum, and the main active substance is steroid called gulgul sterone. It has been known to inhibit the synthesis of cholesterol in the liver by acting as a blocking agent of the fenezoid X receptor (FXR), and recently it has been known that cholesterol Studies have shown that the total amount does not decrease and raises the level of 'low density lipoprotein', a harmful cholesterol in many people.

Terminalia Chebula is known as a panacea in India's traditional Ayurvedic medicine system, originating in India, Sri Lanka, Southeast Asia and China, and is well known for curing blindness. Treatment of infectious diseases, chronic ulcers, treatment of skin fungal infections, anti-aging, immunity enhancement.

The winter cherry root (Withania Somnifera Root (Ashwaganda)) is native to India and has the Sanskrit word "smell of horse". Indian ginseng, called the King of Herbs, is one of the most important Ayurvedic herbs used in India for over 4,000 years. The main component of the steroidal alkaloid, ideoloid, is similar in function and structure to ginsenosides, the main component of ginseng. It is mainly used to help recovery after disease, or to treat tonic, tumors, inflammation and infectious diseases.

Andrographis paniculata is very cold and cold, which is the origin of India and Sri Lanka. The traditional Ayurvedic medicine system in India and Sri Lanka has the nickname "Wonder drug" and is usually used for laxatives, trauma treatment, antipyretic, anti-inflammation, digestion, and insect repellent. Andrographolide is a major component of Tenshin, and it is contained in a lot of leaves. It is known as a drug excellent in hepatotoxicity protection and has recently been studied as a pharmacologically active drug. It is also widely used in human cancer, immune cell treatment, cell activation process research, and drug targeting research. The LD50 of andrographolide is 11.46 gm / kg (ip).

Indian gooseberry (Phyllanthus Emblica) is native to India, western Afghanistan and Myanmar, also known as Indian gooseberry, and has the same name as tree and fruit. It is bitter and has a high content of vitamin C. The main ingredients are vitamin C, tannins, flavonoids, camphorol, ellagic acid, and gallic acid. It is thought that it will have antioxidant effect by vitamin C, but there is also opinion that it will have antioxidant effect by tannin content. It is mainly used for protection of hair, scalp and hair, removal of wrinkles, treatment of constipation, prevention of stroke and so on.

The extract of the indigenous natural product used in the present invention can be prepared by using a solvent under the conditions of temperature and pressure according to a conventional method as described below.

That is, the extract of the present invention is obtained by extracting from various organs or parts (for example, leaves, flowers, roots, stems and shells) of natural products. The extract of the present invention can be produced by a conventional method known in the art . That is, various extraction solvents such as water, anhydrous or lower alcohols having 1-4 carbon atoms (methanol, ethanol, propanol and butanol), acetone, ethyl acetate, chloroform, 1,3-butylene glycol and butyl acetate Can be obtained from natural products using an extraction solvent selected from the group consisting of.

It is apparent to those skilled in the art that the extract of the present invention can be obtained not only by the above-mentioned extraction solvent but also by using other extraction solvent, which exhibits substantially the same effect. In addition, the extract of the present invention includes not only the extract obtained by the above-mentioned extraction solvent, but also an extract obtained through a conventional purification process. For example, by separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatographies (made for separation by size, charge, hydrophobicity or affinity), and the like, The active fraction is also included in the extract of the present invention.

Meanwhile, the extract of the present invention can be preferably extracted in the same manner as the following Reference Examples 1 to 5.

The Indian Bethelium resin extract of the present invention is contained in an amount of 0.0001 to 20% by weight based on the total weight of the composition. If the content of the extract is less than 0.0001% by weight, the skin effect can not be sufficiently obtained. If the content of the extract is more than 20% by weight, the suitability for formulation may be decreased and the effect of increasing the content may not be increased.

The GAZa extract of the present invention is contained in an amount of 0.0001 to 20% by weight based on the total weight of the composition. If the content of the extract is less than 0.0001% by weight, the skin effect can not be sufficiently obtained. If the content of the extract is more than 20% by weight, the suitability for formulation may be decreased and the effect of increasing the content may not be increased.

The winter cherry root extract of the present invention is contained in an amount of 0.0001 to 20% by weight based on the total weight of the composition. If the content of the extract is less than 0.0001% by weight, the skin effect can not be sufficiently obtained. If the content of the extract is more than 20% by weight, the suitability for formulation may be decreased and the effect of increasing the content may not be increased.

The tsuyoshi extract of the present invention is contained in an amount of 0.0001 to 20% by weight based on the total weight of the composition. If the content of the extract is less than 0.0001% by weight, the skin effect can not be sufficiently obtained. If the content of the extract is more than 20% by weight, the suitability for formulation may be decreased and the effect of increasing the content may not be increased.

The Indian gooseberry extract of the present invention is contained in an amount of 0.0001 to 20% by weight based on the total weight of the composition. If the content of the extract is less than 0.0001% by weight, the skin effect can not be sufficiently obtained. If the content of the extract is more than 20% by weight, the suitability for formulation may be decreased and the effect of increasing the content may not be increased.

When the composition of the present invention is contained in the above range, it is not only suitable for exhibiting the intended effect of the present invention but also can satisfy both the stability and safety of the composition and may be suitably contained in the above range in terms of cost effectiveness .

The above-mentioned winter cherry root extract, tschimen extract and indian gooseberry extract have a limited effect on the concentration of natural products due to their physical and chemical properties, but they have an effect-enhancing effect even when mixed in small amounts.

It is preferable that the extracts of Indian Bethelium resin, Gadja extract, Winter cherry root extract, Tepsi extract and Indian gooseberry extract are contained in a weight ratio of 1 to 20: 1 to 20: 1 to 5: 1 to 5: 1 to 5 , More preferably in a weight ratio of 11: 11: 1: 1: 1.

Hereinafter, the present invention will be described in more detail with reference to Examples and Test Examples, but the scope of the present invention is not limited to these examples.

[Referential Example 1] Preparation of Indian Bethelium resin extract (C)

To 1 kg of the Indian Betelrium resin, 5 L of a 100% aqueous ethanol solution was added, and the mixture was refluxed three times and then immersed at 15 DEG C for 3 days. Thereafter, the residue and the filtrate were separated by filtration through a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain 10 g of an Indian Bethelium resin extract.

[Referential Example 2] Preparation of Gadja Extract (T)

To 1 kg of lettuce, 5 L of a 70% aqueous ethanol solution was added, and the mixture was refluxed three times and then immersed at 15 DEG C for 1 day. Thereafter, the residue and the filtrate were separated by filtration through a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain 50 g of a Ghad extract.

[Referential Example 3] Preparation of Tenshi extract (A)

5 kg of 70% ethanol aqueous solution was added to 1 kg of Toshi-In, and the mixture was refluxed three times and then immersed at 15 ° C for 1 day. Thereafter, the residue and filtrate were separated by filtration through a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain 25 g of a crude extract.

[Reference Example 4] Preparation of winter cherry root extract (W)

5 kg of a 70% aqueous ethanol solution was added to 1 kg of raw cherry root, refluxed three times, and then immersed at 15 ° C for 1 day. Thereafter, the residue and the filtrate were separated by filtration through a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain 30 g of a raw cherry root extract.

 [Reference Example 5] Preparation of Indian gooseberry extract (P)

To 1 kg of Indian gooseberry, 5 L of a 70% ethanol aqueous solution was added, and the mixture was refluxed three times and then immersed at 15 DEG C for 1 day. Thereafter, the residue and filtrate were separated by filtration through a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain 25 g of an Indian gooseberry extract.

[Test Example 1] Test for inhibiting tyrosinase activity

In order to confirm the whitening effect of the composition according to the present invention, the mushroom tyrosinase activity assay was used.

Enzyme tyrosinase was extracted from mushroom and was obtained from Sigma (SIGMA). First, tyrosine, a substrate, was dissolved in distilled water to prepare a 0.3 mg / ml solution. The solution was put into a 1.0 ml test tube, and 1.0 ml of a potassium-phosphate buffer solution (0.1 molar concentration, pH 6.8) and 0.7 ml of distilled water were added Solution.

(Comparative Example 3), Winter cherry root extract (Comparative Example 4), and Indian gooseberry extract (Comparative Example 5) as indigenous natural products and Indian Bethelium resin extract (Comparative Example 1) Each of the mixtures (Examples 1 to 4) was dissolved in an ethanol solution at a treatment concentration of 100 ug / ml, 10 ug / ml and 1 ug / ml, respectively, and 0.2 ml of the sample solutions were added to the mixed solution. . As a negative control, only 0.2 ml of the solvent was substituted for each extract sample, and kojic acid (KA), which is known as a tyrosinase inhibitor, was used as a positive control. 0.1 ml of a 2500 unit / ml tyrosinase solution was added to the reaction solution, and the mixture was reacted for 10 minutes in a 37 ° C thermostat. Next, the test tube containing the reaction solution was placed in ice water to quench the reaction, and absorbance at a wavelength of 475 nm was measured with a photoelectric spectrometer. The tyrosinase activity of each extract was calculated according to Equation (1), and the results are shown in Fig.

(Comparative Example 1), Gadja Extract (Comparative Example 2), Astragalus extract (Comparative Example 3), Winter cherry root extract (Comparative Example 4) and Indian gooseberry extract (Comparative Example 1) 5) were used alone, tyrosinase activity was not inhibited except tyrosinase activity of 100 ug / ml, and tyrosinase activity rate was similar to negative control. Therefore, it was confirmed that there was almost no whitening effect when each extract was used alone.

On the other hand, as shown in FIG. 1, when the Indian Bethelium resin extract having no inhibitory effect was mixed with the Gadja extract at various ratios (Examples 1 to 3), the inhibitory effect of tyrosinase activity Was found. In addition, it was found that the winter cherry root extract, Tessin's extract and Indian gooseberry extract were further added (Example 4) to further enhance the inhibitory effect and have excellent whitening efficacy.

Claims (5)

Commiphora Mukul Resin Extract and Terminalia Chebula Extract. The whitening cosmetic composition according to claim 1, further comprising: Winteria cherry root extract, Andrographis paniculata extract and Phyllanthus emblica extract. [Claim 2] The cosmetic composition for whitening as claimed in claim 1, wherein the Indian Bethelium resin extract and Gadja extract are contained in an amount of 0.0001 to 20 wt% based on the total weight of the composition. 3. The whitening cosmetic composition according to claim 2, wherein the winter cherry root extract, astringent seed extract and Indian gooseberry extract are contained in an amount of 0.0001 to 20% by weight based on the total weight of the composition. [3] The composition according to claim 2, wherein the indian benzyl alcohol extract has a weight ratio of 1: 20: 1 to 20: 1 to 5: 1 to 5: 1 to 5 ≪ / RTI > by weight of the composition.

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